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1.
World J Gastroenterol ; 26(5): 535-549, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32089629

RESUMO

BACKGROUND: There are few effective tools to predict survival in patients with invasive intraductal papillary mucinous neoplasms of the pancreas. AIM: To develop comprehensive nomograms to individually estimate the survival outcome of patients with invasive intraductal papillary mucinous neoplasms of the pancreas. METHODS: Data of 1219 patients with invasive intraductal papillary mucinous neoplasms after resection were extracted from the Surveillance, Epidemiology, and End Results database, and randomly divided into the training (n = 853) and the validation (n = 366) cohorts. Based on the Cox regression model, nomograms were constructed to predict overall survival and cancer-specific survival for an individual patient. The performance of the nomograms was measured according to discrimination, calibration, and clinical utility. Moreover, we compared the predictive accuracy of the nomograms with that of the traditional staging system. RESULTS: In the training cohort, age, marital status, histological type, T stage, N stage, M stage, and chemotherapy were selected to construct nomograms. Compared with the American Joint Committee on Cancer 7th staging system, the nomograms were generally more discriminative. The nomograms passed the calibration steps by showing high consistency between actual probability and nomogram prediction. Categorial net classification improvements and integrated discrimination improvements suggested that the predictive accuracy of the nomograms exceeded that of the American Joint Committee on Cancer staging system. With respect to decision curve analyses, the nomograms exhibited more preferable net benefit gains than the staging system across a wide range of threshold probabilities. CONCLUSION: The nomograms show improved predictive accuracy, discrimination capability, and clinical utility, which can be used as reliable tools for risk classification and treatment recommendations.

2.
Arch Environ Contam Toxicol ; 78(4): 545-554, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31915850

RESUMO

Chemicals pollution in the environment has attracted attention all over the world, and the toxicity prediction of chemical pollutants has become quite important. In this paper, we introduce a simple approach to predict the toxicity of some chemical components, in which the Tchebichef image moment (TM) method was employed to extract useful chemical information from the images of molecular structures to establish quantitative structure-activity relationship (QSAR) prediction models. The proposed approach was applied to predict the toxicity of anilines and phenols for the aquatic organisms of P. subcapitata and V. fischeri, in which the obtained TMs were defined as the independent variables, while the biological toxicity (pEC50) was regarded to be the dependent variable. Then, the predictive models were established by stepwise regression, respectively. The obtained squared correlation coefficients of leave-one-out cross-validation (Q2) for training sets and the predictive squared correlation coefficients (Rp2) for test sets of the two groups of data were higher than 0.79 and 0.75, respectively, which indicated that the obtained models possessed satisfactory accuracy and reliability. Compared with several reported methods, the proposed approach was more convenient and has a higher predictive capability. Our study provides another perspective in QSAR research.


Assuntos
Compostos de Anilina/toxicidade , Organismos Aquáticos/efeitos dos fármacos , Modelos Teóricos , Fenóis/toxicidade , Poluentes Químicos da Água/toxicidade , Aliivibrio fischeri/efeitos dos fármacos , Compostos de Anilina/química , Clorófitas/efeitos dos fármacos , Fenóis/química , Valor Preditivo dos Testes , Relação Quantitativa Estrutura-Atividade , Reprodutibilidade dos Testes , Poluentes Químicos da Água/química
3.
Chem Biol Drug Des ; 95(2): 240-247, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31623027

RESUMO

Non-structural viral protein 5B (NS5B) is a viral protein in hepatitis C virus. Although various inhibitors against NS5B have been found, the activity prediction of similar untested inhibitors is still highly desirable. In this respect, the Tchebichef moments (TMs) calculated from the images of molecular structures were regarded as the independent variables while the inhibitory activity (pIC50 ) was the dependent variable, and the predictive model was established by means of stepwise regression. The R-squared of leave-one-out cross-validation (Q2 ) for the training set and the R-squared of prediction ( R p 2 ) for external independent test set were 0.919 and 0.927, respectively. The obtained model was also evaluated strictly. Compared with the multivariate curve resolution with alternating least squares (MCR-ALS) and the QSAR approaches derived from the literature, the proposed method is more accurate and reliable. This study not only provides an effective approach to predict the biological activity of RNA replication's inhibitors, but also extends the QSAR modeling technique.

4.
J Nanosci Nanotechnol ; 20(5): 2832-2839, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635619

RESUMO

Magnetic NiFe2O4 nanoparticles were successfully prepared via the coprecipitation process with ferric nitrate, nickel nitrate, and sodium hydroxide as raw materials, and they were characterized by the scanning electron microscopy (SEM), the transmission electron microscope (TEM), the X-ray diffraction (XRD), the vibrating sample magnetometer (VSM), the energy dispersive spectroscopy (EDS) and the Brunauer-Emmett-Teller (BET). The magnetic NiFe2O4 nanoparticles were employed to research the adsorption performance of reactive red 2BF (RR-2BF) onto them, and they revealed very large adsorption capacity of RR-2BF. The adsorption kinetics data were evaluated with the pseudo first-order, the pseudo second-order and the intraparticle diffusion models, and the results showed that the pseudo second-order kinetics model was best fitted for the adsorption of RR-2BF onto magnetic NiFe2O4 nanoparticles. While, the adsorption isotherm experiments were carried out at room temperature, and the experimental data conformed to the Langmuir model, which suggested that the adsorption of RR-2BF onto magnetic NiFe2O4 nanoparticles was the monolayer adsorption process. The magnetic NiFe2O4 nanoparticles revealed good reusability, and the adsorption capacity was maintained over 80% of the initial capacity after being used for five cycles.

5.
J Chem Inf Model ; 59(10): 4159-4166, 2019 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-31525291

RESUMO

To extract the features in first-order or second-order signals, the two kinds of discrete Shmaliy moment (DSM) methods were proposed and applied to the quantitative analysis of multitarget compounds in complexes based on the UV-vis and high-performance liquid chromatography with pulsed amperometric detector (HPLC-PAD) spectra of samples for the first time. All the statistical parameters demonstrated that the obtained models were accurate and the established analytical methods were reliable, even in the presence of a different degree of overlapping signals as well as various interferences. Compared with Tchebichef moment (TM) and other classical methods such as multivariate curve resolution-alternating least-squares (MCR-ALS), partial least-squares (PLS) regression, and N-way partial least-squares (N-PLS), the proposed methods are more convenient and efficient, which not only provides another suitable tool for the quantitative analysis of multitarget components in complex samples but also extends the application of moment invariants in chemical signal analyses.

6.
Dis Markers ; 2019: 6468729, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31275449

RESUMO

Objective: The prognostic role of Klotho in patients with chronic kidney disease is still controversial. Therefore, we performed this meta-analysis to assess the relationship between the low sKlotho level and the risk of adverse kidney outcomes. Materials and Methods: We systematically searched medical databases, such as PubMed, Embase, and the Cochrane Library, for eligible publications regarding the relationship between the low sKlotho level and risk of adverse kidney outcomes. The quality of included studies was assessed by using the Newcastle-Ottawa Scale. Combined hazard ratios (HRs) and its 95% confidence intervals (CIs) were calculated using a random- or fixed-effect model. Subgroup analysis was conducted with stratification by age, estimated glomerular filtration rate (eGFR), follow-up interval, region, and study quality. All data was analyzed by RevMan 5.3 analysis software. Results: Eight cohort studies with 3586 participants from 3818 studies were included in our final analysis. Levels of sKlotho were significantly correlated with the eGFR, with a summary correlation coefficient r and 95% CI of 0.469 (0.226, 0.658). Additionally, low sKlotho levels were strongly associated with increased adverse kidney outcomes, and the pooled HR and its 95% CI were 1.64 (1.19, 2.26; P = 0.002), despite publication bias and statistical heterogeneity (I 2 = 52%, P = 0.07). Furthermore, this positive correlation was still observed in all of the subgroup analyses. However, heterogeneity was present in subgroup analyses stratified by the eGFR and follow-up interval. Conclusion: Levels of sKlotho are positively correlated with the eGFR, and low sKlotho levels are significantly associated with an increased risk of poor kidney outcomes. Therefore, sKlotho could be used as a novel biomarker for early diagnosis and prognostic assessment for patients with chronic kidney disease. Studies with a larger sample size and longer follow-up period are warranted to validate our results.


Assuntos
Glucuronidase/sangue , Insuficiência Renal Crônica/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Taxa de Filtração Glomerular , Humanos , Pessoa de Meia-Idade , Insuficiência Renal Crônica/fisiopatologia
7.
Analyst ; 144(15): 4575-4581, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31233043

RESUMO

Although a large number of fluorescent probes have been developed, the simultaneous quantitative analysis of intracellular thiols is still difficult due to the spectral overlap and the complexity of the intracellular environment. In this study, a multi-signal fluorescent probe was employed for the simultaneous quantification of intracellular glutathione (GSH), cysteine (Cys) and homocysteine (Hcy). As the feature variables of the target components, the Tchebichef image moments (TMs) calculated from the grayscale images of the 3D fluorescence spectra were used to establish the quantitative linear models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 5.6% and 8.7%, respectively. The recoveries ranged from 97.0% to 105.9%. In addition, the proposed approach was applied to the simultaneous quantitative determination of Cys, GSH and Hcy in the MCF-10A cell (a type of normal cell) and MDA-MB-231 cancer cell. The obtained results indicated that the concentrations of the three thiols in the cancer cell were higher than those in the normal cell. This study not only provides an effective approach for the quantification of multi-target bio-molecules in complicated intracellular environments, but also further extends the applications of multi-signal fluorescent probes, which will promote the design of new multi-signal fluorescent probes.


Assuntos
Benzotiazóis/química , Cumarínicos/química , Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Linhagem Celular Tumoral , Humanos , Análise dos Mínimos Quadrados , Limite de Detecção , Modelos Químicos , Análise de Componente Principal , Espectrometria de Fluorescência/métodos
8.
Food Chem ; 290: 72-78, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31000058

RESUMO

Diverse interferences often affect the determination of pesticide residues in various kinds of food, and complex pretreatments are necessary. An effective approach for the simultaneous quantitative analysis of multi-target components in different substrates was proposed, and applied to the determination of ternary pesticides in cherry tomatoes and red grape samples. By means of HPLC-DAD, the spectra were obtained under isocratic elution. Utilizing the Tchebichef image moments, unified quantitative models were established for the three target components in the samples with two different substrates, respectively. The correlation coefficients of leave-one-out cross-validation (Rloo-cv2) of the obtained models were more than 0.9975. The predictive correlation coefficients (Rp2) were more than 0.9831. Compared with the N-PLS and MCR-ALS methods, the proposed approach is not only more accurate and reliable, but also can extract essential information and expected to be a potential tool to rapidly quantify multi-component in different substrates without complex pretreatments.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Lycopersicon esculentum/química , Resíduos de Praguicidas/análise , Vitis/química , Cromatografia Líquida de Alta Pressão/métodos , Fatores de Tempo
9.
Steroids ; 143: 1-5, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30543815

RESUMO

Three pairs of ginsenoside epimers, including three new compounds (2, 3 and 5), were isolated from the flower buds of Panax ginseng. The structures of the isolated compounds were elucidated on the basis of considerable spectroscopic analyses and comparison with the reported data. All six compounds were evaluated for their cytotoxicties against three human cancer cell lines, HL-60, MGC80-3 and Hep-G2. Compounds 1, 3, and 6 with S configurations at C-24 or C-20 showed moderate inhibitory activities with IC50 values of 25.32, 18.76, and 38.64 µM in HL-60 cells, respectively. Our findings showed that different configurations of these isolated ginsenosides had a significant impact on the antitumor activity, and S epimers were higher than R.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Flores/química , Ginsenosídeos/química , Ginsenosídeos/farmacologia , Panax/química , Linhagem Celular Tumoral , Humanos , Hidrólise , Estereoisomerismo
10.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1097-1098: 128-141, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30241074

RESUMO

Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by dysfunction of glycolipid metabolism. YLTZ is used to treat hyperlipidemia, yet its hypolipidemic and hypoglycemic mechanism on T2DM are unknown. Thus, UPLC/TOF/MS was applied in this study to identify the potential bio-markers, and deduce the possible metabolic pathways. According to bio-indexes, the increased blood lipid levels, including TC, TG, LDL and FA, and the decreased HDL, the elevated glucose, reduced insulin level and impaired OGTT were observed in diabetic rat model. While YLTZ can decrease the lipid levels and glucose content, as well as increased insulin standards and improve OGTT. After data from UPLC/TOF/MS processed, 17 metabolites were obtained, including phospholipids (LPCs, PCs and PGP (18:1)), beta-oxidation production (HAA, VAG and CNE) and precursors (THA), bile acid (CA, CDCA and IDCA), hydrolysate of TG (MG (22:4)), glycometabolism (G6P), cholesterol-driven synthetics (ADO) and production of arachidonate acid (THETA). As a result, YLTZ was able to reduce LPCs, PCs, PGP (18:1), HAA, VAG, CNE, CA, ADO and THETA, as well as enhance MG (22:4) and G6P. After analyzing results, several metabolic pathways were deduced, which containing, cholesterol synthesis and elimination, FA beta-oxidation, TG hydrolysis, phospholipids synthesis, glycolysis, gluconeogenesis and inflammation. Consequently, YLTZ performed to prohibit the FA beta-oxidation, synthesis of cholesterol and phospholipids, gluconeogenesis and inflammation level, as well as promote TG hydrolysis, glycolysis and blood circulation. Hence, applying metabonomics in TCM research can uncover its pharmacological edges, elucidating comprehensively that YLTZ has capacity of hypolipidemic, hypoglycemic and promoting blood circulation, matching the effect of removing blood stasis, eliminating phlegm and dampness.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Ginkgo biloba , Glicolipídeos/metabolismo , Gynostemma , Própole/farmacologia , Animais , Biomarcadores/sangue , Biomarcadores/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Glicolipídeos/sangue , Metabolismo dos Lipídeos/efeitos dos fármacos , Masculino , Espectrometria de Massas/métodos , Metaboloma/efeitos dos fármacos , Metabolômica/métodos , Fitoterapia/métodos , Própole/química , Ratos , Ratos Wistar
11.
Proteins ; 86(7): 751-758, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29675929

RESUMO

Circular dichroism (CD) spectroscopy is a widely used technique for the evaluation of protein secondary structures that has a significant impact for the understanding of molecular biology. However, the quantitative analysis of protein secondary structures based on CD spectra is still a hard work due to the serious overlap of the spectra corresponding to different structural motifs. Here, Tchebichef image moment (TM) approach is introduced for the first time, which can effectively extract the chemical features in CD spectra for the quantitative analysis of protein secondary structures. The proposed approach was applied to analyze reference set and the obtained results were evaluated by the strict statistical parameters such as correlation coefficient, cross-validation correlation coefficient and root mean squared error. Compared with several specialized prediction methods, TM approach provided satisfactory results, especially for turns and unordered structures. Our study indicates that TM approach can be regarded as a feasible tool for the analysis of the secondary structures of proteins based on CD spectra. An available TMs package is provided and can be used directly for secondary structures prediction.


Assuntos
Dicroísmo Circular , Estrutura Secundária de Proteína , Proteínas/química , Bases de Dados de Proteínas
12.
Biol Pharm Bull ; 41(4): 585-591, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29607931

RESUMO

Ginsenoside-Rg1 (G-Rg1) is an agent isolated from Panax ginseng that exerts anti-fibrotic effects; however, the mechanism is still unclear. Herein, we investigated whether G-Rg1 administration can mitigate or reverse unilateral ureteral obstruction (UUO)-induced renal fibrosis by regulating the Klotho/transforming growth factor (TGF)-ß1/Smad signaling pathway in rats. Sprague-Dawley male rats were subjected to UUO, and rats in the treatment group were administered G-Rg1 or G-Rg1 plus Klotho short hairpin RNA interference (shRNA), while rats in the control and model groups were administered vehicle for 14 d. Epithelial-mesenchymal transition (EMT) biomarkers and Klotho/TGF-ß1 signaling molecules were examined by immunohistochemistry, quantitative real-time PCR and Western blotting. Immunohistochemistry showed that UUO induced increased pro-fibrotic TGF-ß1 expression, overexpression of the mesenchymal marker, α-smooth muscle actin (α-SMA), and suppression of the epithelial marker, E-cadherin. Moreover, Western blotting analysis indicated that UUO promoted TGF-ß1 and phosphorylated Smad3 (p-Smad3) expression (p<0.01), but blocked Klotho and Smad7 expression (p<0.01). After G-Rg1 administration, the UUO-induced TGF-ß1 and p-Smad3 expression was suppressed (p<0.01), whereas the reduced Klotho and Smad7 expression was reversed (p<0.05), followed by amelioration of the EMT process. Intriguingly, the G-Rg1 effects were largely abrogated by Klotho knockdown. Furthermore, Klotho expression was upregulated by G-Rg1 treatment at the mRNA and protein levels. Our results suggest that G-Rg1 may be beneficial for ameliorating renal fibrosis by targeting Klotho/TGF-ß1/Smad signaling in UUO rats.


Assuntos
Ginsenosídeos/farmacologia , Nefropatias/metabolismo , Substâncias Protetoras/farmacologia , Animais , Fibrose , Ginsenosídeos/uso terapêutico , Glucuronidase/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Rim/patologia , Nefropatias/tratamento farmacológico , Masculino , Substâncias Protetoras/uso terapêutico , Ratos Sprague-Dawley , Transdução de Sinais , Proteínas Smad/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Obstrução Ureteral/complicações
13.
J Investig Med ; 66(3): 669-675, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29061648

RESUMO

To investigate whether the soluble Klotho (s-Klotho) level in patients with chronic kidney disease (CKD) is related to kidney function and whether a low s-Klotho level can predict adverse renal outcomes or CKD progression in patients with advanced CKD. 112 patients with CKD stages 3-5 and 30 healthy volunteers were enrolled. Blood samples were collected to measure serum creatinine, calcium, phosphorus, intact parathyroid hormone, and hemoglobin. s-Klotho and fibroblast growth factor 23 (FGF23) were determined by ELISA. We first conducted a cross-sectional study to investigate correlations between s-Klotho and estimated glomerular filtration rate (eGFR) and other parameters. Patients were then followed prospectively for 20.1±10.1 months according to s-Klotho median level until serum creatinine doubled, or initiation of renal replacement therapy, or death. s-Klotho levels inpatients with CKD were significantly lower than that in the control group. For patients with CKD, there were no differences in age distribution among subgroups. However, s-Klotho level differed significantly across CKD stages, and it was lower in the advanced CKD group compared with the moderate CKD group. Correlation analysis revealed that s-Klotho was positively associated with eGFR, but inversely associated with FGF23. During the follow-up of 20.1±10.1 months, patients with higher s-Klotho levels showed a reduced risk of kidney adverse outcomes, including serum creatinine doubling and initiation of renal replacement therapy. Cox regression analysis revealed that low s-Klotho was an independent risk factor for CKD progression. s-Klotho level was closely correlated with kidney function, further, low s-Klotho level could predict adverse kidney disease outcomes in patients with progressive CKD.


Assuntos
Glucuronidase/sangue , Rim/fisiopatologia , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/fisiopatologia , Estudos de Casos e Controles , Demografia , Feminino , Taxa de Filtração Glomerular , Humanos , Estimativa de Kaplan-Meier , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Modelos de Riscos Proporcionais , Solubilidade , Resultado do Tratamento
14.
Brain Behav ; 7(11): e00849, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-29201550

RESUMO

Background and Purpose: Dehydration was found to be involved in the poor prognosis of patients with acute ischemic stroke. It is unclear whether dehydration status before onset is related with prognosis of thrombolysed patients with acute ischemic stroke. If it is the case, quickly hydrating may improve the prognosis. The present study was designed to explore the issue. Methods: Eligible 294 patients with acute ischemic stroke after thrombolysis were enrolled in the present study according to inclusion/exclusion criteria. According to the modified Rankin scale (mRS) 90 days post stroke, the patients were divided into two groups: mRS 0-2 (n = 191) and mRS 3-6 (n = 103). In the present study, BUN/Cr ≥ 15 combined with USG > 1.010 or either of them were chosen as dehydration marker. Clinical data were analyzed between two groups. Univariate and multivariate statistical analyses were carried out. Results: Age, fibrinogen, blood glucose, BUN/Cr, NIHSS score at admission, the systolic blood pressure (SBP) before thrombolysis, dehydration status (BUN/Cr ≥ 15 plus USG > 1.010), hyperlipidemia, USG and D-dimer on admission day, and TOAST classification showed significant difference between two groups (p < .05). Further stratification analysis showed that BUN/Cr ≥ 15, NIHSS ≥ 6, blood glucose ≥8, and SBP > 150 were markedly associated with poor outcome (mRS 3-6, p < .05). After adjusting for age, fibrinogen, USG, D-dimer, dehydration status, NIHSS, blood glucose, SBP, hyperlipidemia, and BUN/Cr at admission, multivariate logistic regression showed that dehydration status, higher NIHSS, higher blood glucose, and higher SBP at admission were independent risk factors for predicting the long-term poor prognosis of thrombolysed patients. Conclusions: The present findings suggest that BUN/Cr ≥ 15 combined with USG > 1.010 as a marker of dehydration status was an independent risk factor for long-term poor prognosis of thrombolysed patients with acute ischemic stroke.


Assuntos
Infarto Cerebral/tratamento farmacológico , Infarto Cerebral/fisiopatologia , Desidratação/complicações , Desidratação/fisiopatologia , Terapia Trombolítica , Doença Aguda , Idoso , Glicemia/metabolismo , Pressão Sanguínea/fisiologia , Nitrogênio da Ureia Sanguínea , Creatina/sangue , Feminino , Seguimentos , Humanos , Masculino , Prognóstico , Estudos Prospectivos , Proteínas Recombinantes/uso terapêutico , Fatores de Risco , Ativador de Plasminogênio Tecidual/uso terapêutico
15.
Environ Sci Technol ; 51(23): 13816-13824, 2017 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-29121473

RESUMO

Efficient separation and preconcentration of trace nanoparticulate silver (NAg) from large-volume environmental waters is a prerequisite for reliable analysis and therefore understanding the environmental processes of silver nanoparticles (AgNPs). Herein, we report the novel use of polyvinylidene fluoride (PVDF) filter membrane for disk-based solid phase extraction (SPE) of NAg in 1 L of water samples with the disk-based SPE system, which consists of a syringe pump and a syringe filter holder to embed the filter membrane. While the PVDF membrane can selectively adsorb NAg in the presence of Ag+, aqueous solution of 2% (m/v) FL-70 is found to efficiently elute NAg. Analysis of NAg is performed following optimization of filter membrane and elution conditions with an enrichment factor of 1000. Additionally, transmission electron microscopy (TEM), UV-vis spectroscopy, and size-exclusion chromatography coupled with ICP-MS (SEC-ICP-MS) analysis showed that the extraction gives rise to no change in NAg size or shape, making this method attractive for practical applications. Furthermore, feasibility of the protocol is verified by applying it to extract NAg in four real waters with recoveries of 62.2-80.2% at 0.056-0.58 µg/L spiked levels. This work will facilitate robust studies of trace NAg transformation and their hazard assessments in the environment.


Assuntos
Nanopartículas Metálicas , Polivinil , Poluentes Químicos da Água , Prata , Extração em Fase Sólida
16.
Sheng Li Xue Bao ; 69(5): 693-702, 2017 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-29063117

RESUMO

In the daily life, we perceive the world around us by integrating multiple sensory cues (visual, auditory, olfactory, gustatory, tactile, vestibular and proprioceptive) to create a coherent, reliable representation that allows us to interact meaningfully with the environment. The integration of different sensory information is necessary for our perception, motor transformation, decision making, learning and memory. In the past decades, many interdisciplinary researchers have been attracted to the field of multisensory research, and tremendous advances have been made in this field. We review the researches on multisensory integration during self-motion perception in the past decades from the candidate areas, the integration principles and the neural correlation of the behaviors, with the intention to provide a comprehensive source for those interested in understanding the neural substrates for multisensory integration. Meanwhile, we also provide a prospect for the future research in this field.


Assuntos
Percepção de Movimento/fisiologia , Sensação/fisiologia , Humanos
17.
J Huazhong Univ Sci Technolog Med Sci ; 37(3): 462-468, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28585132

RESUMO

The prognostic value of phosphatidylinositol-4, 5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) in patients with esophageal squamous cell carcinoma (ESCC) is controversial. We aimed to investigate the prognostic significance of PIK3CA mutation in patients with ESCC. EMBASE, PubMed, and Web of Science databases were systematically searched from inception through Oct. 3, 2016. The hazard ratios (HRs) and 95% confidence intervals (CI) were calculated using a random effects model for overall survival (OS) and disease-free survival (DFS). Seven studies enrolling 1505 patients were eligible for inclusion of the current meta-analysis. Results revealed that PIK3CA mutation was not significantly associated with OS (HR: 0.90, 95% CI: 0.63-1.30, P=0.591), with a significant heterogeneity (I 2=65.7%, P=0.012). Additionally, subgroup analyses were further conducted according to various variables, such as types of specimen, the sample size, technique and statistical methodology. All results suggested that no significant relationship was found between PIK3CA mutation and OS in patients with ESCC. For DFS, there was no significant association between PIK3CA mutation and DFS in patients with ESCC (HR: 1.00, 95% CI=0.47-2.11, P=0.993, I 2=73.7%). Publication bias was not present and the results of sensitivity analysis were very stable in the current meta-analysis. Our findings suggest that PIK3CA mutation has no significant effects on OS and DFS in ESCC patients. More well-designed prospective studies with better methodology for PIK3CA assessment are required to clarify the prognostic significance of PIK3CA mutation in ESCC patients.


Assuntos
Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/genética , Classe I de Fosfatidilinositol 3-Quinases/genética , Neoplasias Esofágicas/diagnóstico , Neoplasias Esofágicas/genética , Mutação , Carcinoma de Células Escamosas/enzimologia , Carcinoma de Células Escamosas/mortalidade , Classe I de Fosfatidilinositol 3-Quinases/metabolismo , Neoplasias Esofágicas/enzimologia , Neoplasias Esofágicas/mortalidade , Carcinoma de Células Escamosas do Esôfago , Seguimentos , Expressão Gênica , Humanos , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Tamanho da Amostra
18.
Molecules ; 22(3)2017 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-28287442

RESUMO

The present study was designed to simultaneously isolate the less polar ginsenosides from the flower buds of Panax ginseng (FBPG). Five ginsenosides, including a pair of new 20-methoxyl isomers, were extracted from FBPG and purified through a five-step integrated strategy, by combining ultrasonic extraction, Diaion Hp-20 macroporous resin column enrichment, solid phase extraction (SPE), reversed-phase high-performance liquid chromatography (RP-HPLC) analysis and preparation, and nuclear magnetic resonance (NMR) analysis. The quantification of the five ginsenosides was also discussed by a developed method with validations within acceptable limits. Ginsenoside Rg5 showed content of about 1% in FBPG. The results indicated that FBPG might have many different ginsenosides with diverse chemical structures, and the less polar ginsenosides were also important to the quality control and standardization of FBPG.


Assuntos
Flores/química , Ginsenosídeos/química , Ginsenosídeos/isolamento & purificação , Panax/química , Cromatografia Líquida de Alta Pressão , Isomerismo , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Extração em Fase Sólida
19.
Int Urol Nephrol ; 49(2): 345-352, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27796696

RESUMO

PURPOSE: Klotho deficiency is implicated in various kidney diseases, including renal fibrosis. The aim of this study was to investigate the effect of Klotho administration on epithelial-mesenchymal transition (EMT) and renal fibrosis induced by cyclosporine A (CsA) in rats. METHODS: CsA-induced renal fibrosis was established by oral administration of CsA (30 mg/kg) to rats on a low-salt diet for 28 days. Klotho was administered to rats by intraperitoneal injection. Renal pathological changes were evaluated by hematoxylin and eosin and Masson's trichrome staining. The EMT response was assessed by measuring the level of TGF-ß1, E-cadherin and α-SMA by immunohistochemistry and Western blot. RESULTS: Administration of CsA for 28 days induced renal damage, decreased Klotho expression and activated the EMT response (demonstrated as increased TGF-ß1 and α-SMA expression accompanied by decreased in E-cadherin expression). Treatment with Klotho significantly ameliorated pathological lesions of the kidney by modulating the expression of EMT-associated proteins in the kidney. CONCLUSIONS: Klotho inhibits CsA-induced EMT and renal fibrosis in rats. Klotho may serve as a therapeutic agent to minimize CsA-induced renal fibrosis.


Assuntos
Transição Epitelial-Mesenquimal/fisiologia , Glucuronidase/metabolismo , Nefropatias , Animais , Caderinas/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Fibrose , Rim/patologia , Nefropatias/metabolismo , Nefropatias/patologia , Ratos , Fator de Crescimento Transformador beta1/metabolismo
20.
Artigo em Inglês | MEDLINE | ID: mdl-27798124

RESUMO

INTRODUCTION: The renal renin-angiotensin system (RAS) and the ultrasensitive energy sensor AMP-activated protein kinase (AMPK) have been implicated in normal and aberrant states of the kidney, but interaction between the RAS and AMPK remains unknown. METHODS: Ninety-six rats were stratified into four groups: sham, uninephrectomised, uninephrectomised rats treated with the angiotensin-converting enzyme inhibitor lisinopril or the angiotensin receptor blocker losartan. Histopathological examination at 9 months post-operation and biochemical measurements at 3, 6 and 9 months were performed for changes in renal structure and function. The expression of AMPK and angiotensin II at 9 months was detected by immunofluorescence microscopy and western blot. RESULTS: Compared with sham rats, uninephrectomised rats demonstrated progressive glomerulosclerosis, tubular atrophy with cast formation and chronic inflammatory infiltration, in parallel to elevated serum urea, creatinine, urine total protein to creatinine ratio and reduced serum albumin. Overexpression of angiotensin II coexisted with a 85.6% reduction of phosphorylated to total AMPK ratio in the remnant kidney of uninephrectomised rats. RAS blockade by the angiotensin-converting enzyme inhibitor or angiotensin receptor blocker substantially normalised AMPK expression, morphological and functional changes of the remnant kidney. CONCLUSIONS: Uninephrectomy-induced RAS activation and AMPK inhibition in the remnant kidney could be substantially corrected by RAS blockade, suggesting a cross-talk between AMPK and RAS components in uninephrectomised rats.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Nefrectomia , Sistema Renina-Angiotensina , Angiotensina I/metabolismo , Angiotensina II/metabolismo , Animais , Ativação Enzimática , Imunofluorescência , Rim/enzimologia , Rim/patologia , Rim/fisiopatologia , Masculino , Microscopia Confocal , Fosforilação , Ratos Sprague-Dawley
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