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1.
Eur J Pharmacol ; 887: 173559, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32949605

RESUMO

There is little literature showing the effect of urocortin (UCN) on macrophage apoptosis. The underlying mechanism is also unclear. This work was to investigate the involvement of UCN in the regulation of LPS-induced macrophage apoptosis and hence in the prevention from the atherosclerotic lesion development through targeting PLA2. Flow cytometry analysis showed that cell apoptosis was increased by more than 50% after LPS treatment in human THP-1 macrophage. Lp-PLA2 and cPLA2 were found to mediate LPS-induced macrophage apoptosis and NF-κB differentially influenced the expression of Lp-PLA2 and cPLA2. However, the reverse regulation of the expression of Lp-PLA2 and cPLA2 by NF-κB suggested that NF-κB may not be a key target for regulating macrophage apoptosis. Interestingly, we found that the approximate three folds upregulation of cPLA2 was in line with the induction of S1P formation and cell apoptosis by LPS. Inversely, LPS obviously decreased UCN expression by about 50% and secretion by about 25%. Both the enzyme inhibitor and knockdown expression of cPLA2 could completely abolish LPS-induced cell apoptosis. In addition, suppression of S1P synthesis by Sphk1 inhibitor PF-543 reduced the expression of cPLA2 and cell apoptosis but at the same time restored the normal level of UCN in cell culture supernatant. Furthermore, addition of exogenous UCN also reversed LPS-induced expression of cPLA2 and apoptosis. Taken together, UCN may be the reverse regulator of LPS-S1P-cPLA2-apoptosis pathway, thereby contributing to the prevention from the formation of unstable plaques.

2.
J Agric Food Chem ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-32907328

RESUMO

Approximately 50-80% of the world population are infected with H. pylori, which is categorized as a class I carcinogen. Antiadhesive therapy is emerging as a promising alternative to antibiotics against bacterial infection. This study demonstrated that defatted wheat germ protein hydrolysates (DWGPH) effectively inhibited H. pylori adhesion to gastric epithelial cells. DWGPH prepared by pronase possessed the best activity where its inhibitory percentage at 10 mg/mL was 51.7 ± 6.8% and the minimum antiadhesive concentration was 0.31 mg/mL. The antiadhesive activity is attributable to peptides acting as receptor analogs in binding to H. pylori. Peptides with potential H. pylori-binding ability (n = 267) were identified, and their structural characteristics were comprehensively analyzed, including net charge, Boman index, instability index, aliphatic index, molecular weight, isoelectric point, hydrophobicity, and Hmoment (α-helix and ß-sheet). This work provided an array of peptide sequences for further exploration as putative ligands of H. pylori adhesins and for elucidating molecular mechanisms.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32984074

RESUMO

Drug-resistant bacteria infections and drug residues have been increasing and causing antibiotic resistance and public health threats worldwide. Antimicrobial peptides (AMPs) are novel antimicrobial drugs with the potential to solve these problems. Here, a peptide based on our previously studied peptide PMAP-36PW was designed via N-terminal myristoylation and referred to as Myr-36PW. The fatty acid modification provided the as-prepared peptide with good stability and higher antimicrobial activity compared with PMAP-36PW in vitro. Moreover, Myr-36PW exhibited effective anti-biofilm activity against Gram-negative bacteria and may kill bacteria by improving the permeability of their membranes. In addition, the designed peptide Myr-36PW could inhibit the bacterial growth of Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa GIM 1.551 to target organs, decrease the inflammatory damage, show an impressive therapeutic effect on mouse pneumonia and peritonitis experiments, and promote abscess reduction and wound healing in infected mice. These results reveal that Myr-36PW is a promising antimicrobial agent against bacterial infections.

5.
Sci Adv ; 6(22): eaaz7677, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32766441

RESUMO

A persistent enigma is the rarity of polyploidy in animals, compared to its prevalence in plants. Although animal polyploids are thought to experience deleterious genomic chaos during initial polyploidization and subsequent rediploidization processes, this hypothesis has not been tested. We provide an improved reference-quality de novo genome for allotetraploid goldfish whose origin dates to ~15 million years ago. Comprehensive analyses identify changes in subgenomic evolution from asymmetrical oscillation in goldfish and common carp to diverse stabilization and balanced gene expression during continuous rediploidization. The homoeologs are coexpressed in most pathways, and their expression dominance shifts temporally during embryogenesis. Homoeolog expression correlates negatively with alternation of DNA methylation. The results show that allotetraploid cyprinids have a unique strategy for balancing subgenomic stabilization and diversification. Rediploidization process in these fishes provides intriguing insights into genome evolution and function in allopolyploid vertebrates.

6.
Chemosphere ; 263: 127944, 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32854006

RESUMO

Pharmaceutically active compounds (PhACs) have been frequently detected in aquatic environment and raised concerns because of their environmental persistence and potential ecological risk, especially carbamazepine (CBZ), erythromycin (ERY), atenolol (ATL) and clofibric acid (CA). The UV/H2O2 advanced oxidation process was considered as an effective process to remove pharmaceuticals in wastewater. Because of the diverse structure of pharmaceuticals and the various wastewater matrices, this study established two models to predict the degradation of 4 PhACs in wastewater by UV/H2O2. Besides, the degradation pathway and toxicity of 4 PhACs by UV/H2O2 were explored. The degradation of 4 PhACs by UV/H2O2 followed the pseudo first-order kinetics pattern. The degradation rate of pharmaceuticals decreased as CBZ > ATL > CA > ERY. A kinetic model combining the steady state concentrations of HO∙ successfully predicted the degradation process of pharmaceuticals in 14 secondary municipal wastewater effluents. Also, a water matrix prediction model by response surface methodology (RSM) was established to estimate the degradation of pharmaceuticals well. A detailed and systematic comparison of two models in the objectives of models, predicting target contaminants, types of wastewater and parameters of models was made. In addition, the tentative transformation pathways of 4 PhACs by UV/H2O2 were proposed. 4 PhACs after UV/H2O2 treatment enhanced the toxicity, and prolongation of treatment time can reduce the toxicity on the luminescence.

7.
Sci Total Environ ; 742: 140654, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-32721750

RESUMO

The characteristics of dissolved organic matter (DOM) such as chemical composition, molecular weight (MW) distribution and hydrophobic/hydrophilic distribution can affect wastewater treatment efficiency, effluent quality and ecological risk. Fluorescence spectroscopy could provide a quick estimate of DOM characteristics during the monitoring of wastewater treatment plants (WWTPs). In this study, the characteristic and quantitative correlation of DOM from 14 anaerobic-anoxic-oxic (AAO) processes of WWTPs located in different provinces (municipalities) of China were investigated. The results showed that DOM of MW <1 kDa was the largest group of DOM in influent and secondary effluent, and DOM removal increased as the MW increased. Hydrophilic (HPI) fraction and hydrophobic acid (HPO-A) comprised the major portion of DOM in influent and secondary effluent and exhibited the lowest rate of removal. In addition, DOM concentrations in the northern provinces were higher than in the southern provinces, which were related to the water quality, economy and population. There were positive correlations between specific fluorescence intensity (SFI) and the MW <1 kDa, 1-5 kDa and <10 kDa fractions. The smaller the molecular weight, the better the correlation. Strong positive correlations between regional fluorescence proportion (fi) and HPI were found. SFI and fi may be explored as potential indicators of the MW fractions and the hydrophobic/hydrophilic distribution of DOM in AAO processes WWTPs.

8.
Cell Death Dis ; 11(7): 571, 2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32709922

RESUMO

Oxysterol-binding protein like protein 3 (OSBPL3) has been shown involving in the development of several human cancers. However, the relationship between OSBPL3 and colorectal cancer (CRC), particularly the role of OSBPL3 in the proliferation, invasion and metastasis of CRC remains unclear. In this study, we investigated the role of OSBPL3 in CRC and found that its expression was significantly higher in CRC tissues than that in normal tissues. In addition, high expression of OSBPL3 was closely related to poor differentiation, advanced TNM stage and poor prognosis of CRC. Further experiments showed that over-expression of OSBPL3 promoted the proliferation, invasion and metastasis of CRC in vitro and in vivo models. Moreover, we revealed that OSBPL3 promoted CRC progression through activation of RAS signaling pathway. Furthermore, we demonstrated that hypoxia induced factor 1 (HIF-1A) can regulate the expression of OSBPL3 via binding to the hypoxia response element (HRE) in the promoter of OSBPL3. In summary, Upregulation of OSBPL3 by HIF1A promotes colorectal cancer progression through activation of RAS signaling pathway. This novel mechanism provides a comprehensive understanding of both OSBPL3 and the RAS signaling pathway in the progression of CRC and indicates that the HIF1A-OSBPL3-RAS axis is a potential target for early therapeutic intervention in CRC progression.

9.
Huan Jing Ke Xue ; 41(6): 2679-2687, 2020 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608783

RESUMO

Picophytoplankton (<3 µm), comprising picocyanobacteria (PCY) and photosynthetic picoeukaryotes (PPEs), are considerably important in the material circulation and energy flow of aquatic ecosystems. To explore the temporal and spatial variation patterns of picophytoplankton and their correlations with environmental factors in lotic Yangtze-connected lakes, field in-situ investigations were performed on a monthly basis during the wet season (May to August) in 2019 in East Lake Dongting, a Yangtze-connected lake. The results indicated that both the Chla biomass and abundances of picophytoplankton exhibited significant spatial and temporal variability (P<0.05). The picophytoplankton Chla biomass showed an average concentration of 8.52 µg·L-1 and accounted for 41.6% to total phytoplankton on an average. From May to August, Chla biomass of picophytoplankton kept increasing with increasing temperature, especially in the north and south of the lake, and it was the lowest in the east of the lake. PCY dominated picophytoplankton abundance in East Lake Dongting and was 3.4 times the abundance of PPEs on an average. Similar spatial and temporal variation patterns were observed between PCY and PPEs. The abundances of PCY and PPEs both increased first and then decreased during the wet season. Spatially, picophytoplankton showed a trend to migrate from the northern lake to the southern lake from May to July, and the abundance significantly declined in August and peaked mainly in the north of the lake. The analysis results showed that picophytoplankton in East Lake Dongting exhibited significant spatial and temporal variability during the wet season; the water level and N:P ratio were determined to be the most important factors explaining the variation of the abundance proportion of PCY and PPEs.


Assuntos
Ecossistema , Lagos , China , Monitoramento Ambiental , Fotossíntese , Fitoplâncton , Estações do Ano
10.
World J Surg Oncol ; 18(1): 150, 2020 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-32620130

RESUMO

BACKGROUND: Currently, nivolumab and ipilimumab are the most widely used immune checkpoint inhibitors. We performed a meta-analysis to evaluate the efficacy and treatment-related adverse events (TRAEs) of nivolumab plus ipilimumab therapy in cancer treatment. METHODS: We examined data from PubMed, Web of Science, EBSCO, and Cochrane Library. Eleven articles fulfilled our criteria, which we divided into 3 groups: nivolumab plus ipilimumab versus nivolumab (the dose used for monotherapy is 3 mg/kg), nivolumab plus ipilimumab versus ipilimumab (the dose used for monotherapy is 3 mg/kg), and nivolumab 1 mg/kg plus ipilimumab 3 mg/kg (N1I3) versus nivolumab 3 mg/kg plus ipilimumab 1 mg/kg (N3I1). We measured the complete response (CR), partial response (PR), objective response rate (ORR), and TRAEs in any grade and grade 3 or higher. RESULTS: The overall effect estimate favored the combined immunotherapy group in terms of the ORR (RR: 1.40, p < 0.001) and PR (RR: 1.50, p < 0.001) than nivolumab alone. Compared with ipilimumab alone, the combined immunotherapy group had better CR (RR: 4.89, p < 0.001), PR (RR: 2.75, p < 0.001), and ORR (RR: 3.31, p < 0.001). Finally, N1I3 showed better PR (RR: 1.35, p = 0.006) and ORR (RR: 1.21, p = 0.03) than N3I1. The incidence of any TRAEs was similar between both groups (RR: 1.05, p = 0.06). However, the incidence of serious adverse events (grade 3 or higher) was lower in group N3I1 than group N1I3 (RR: 1.51, p < 0.001). CONCLUSION: This meta-analysis showed that the curative effect of nivolumab plus ipilimumab was better than that of nivolumab or ipilimumab monotherapy. In the combined immunotherapy group, N1I3 was more effective than N3I1. Although the side effects were slightly increased in N1I3 group, overall safety was acceptable.

11.
J Ethnopharmacol ; 261: 113061, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-32525065

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: TG-decoction (Tiao Geng decoction) is the extract of a Chinese herb mixture that has been used for treating menopausal symptoms for over 30 years. We have previously reported anti-aging and anti-oxidative effects of the TG-decoction on hypothalamic neurons in ovariectomized (OVX) rats. AIM OF THE STUDY: The present study further investigates the effects of TG-decoction on the prevention of aging-related ultrastructural changes in menopausal hypothalamic neurons and the likely molecular mechanism. MATERIALS AND METHODS: A total of 120 four-month-old female SPF Sprague Dawley rats were divided into six groups. Five groups were ovariectomized (OVX) and one group served as a sham control. Three OVX groups received TG-decoction at three different doses. The remaining two OVX groups served as positive and negative controls by receiving estradiol valerate and saline solution. The sham group received saline. After one month, aging-related ultrastructural alterations in hypothalamic neurons were evaluated using transmission electron microscopy. Nissl staining was used to assess the pathomorphological changes of the hypothalamic neurons. Cell apoptosis was evaluated by TUNEL. Expression of Bcl-2 family genes was studied using qRT-PCR. Expression of the apoptosis-related proteins ASK1, MKK7, JNK, c-Jun, Bax, Casp3 and Bcl-2 was studied using western blotting. RESULTS: Ovariectomy of female rats led to visible damage and aging-like alterations in the mitochondria and endoplasmic reticulum as well as large deposits of lipofuscin in hypothalamic tissue. TG-decoction treatment prevented this visible damage and lipofuscin deposition, increased the number of nerve cells and normally-shaped Nissl bodies, and reduced the number of TUNEL-positive cells. Expression of Bcl-2 gene was increased, while Bax gene reduced. Expression of the proteins ASK1, MKK7, JNK, c-Jun, Bax and Casp3 was reduced, while that of Bcl-2 was increased. CONCLUSION: TG-decoction reduces aging-related ultrastructural changes in hypothalamic neurons, likely by suppressing ASK1/MKK7/JNK-mediated apoptosis in neuronal mitochondria or nuclei.

12.
Sheng Wu Gong Cheng Xue Bao ; 36(6): 1170-1180, 2020 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-32597066

RESUMO

HDA9, a member of the deacetylase family, plays a vital role in regulating plant flowering time through flowering integrator SOC1 and AGL24. However, it remains elusive how HDA9 interacts with SOC1 and AGL24 in flowering time control. Here, HDA9 was cloned in Brassica juncea and then its three active sites were separately replaced with Ala via overlap extension PCR. Thus, mutants of HDA9(D172A), HDA9(H174A) and HDA9(D261A) were constructed and fused into the pGADT7 vector. The yeast one-hybrid assays indicated that HDA9 mutants remained the interactions with the promoters of SOC1 and AGL24. Furthermore, the aforementioned results were confirmed in the dual luciferase assays. Interestingly, the DNA-protein interactions were weakened significantly due to the mutation in the three active sites of HDA9. It suggested that flowering signal integrator SOC1 and AGL24 were regulated by the key amino acid residues of 172th, 174th and 261th in HDA9. Our results provide valuable information for the in-depth study of the biological function and molecular regulation of HDA9 in Brassica juncea flowering time control.


Assuntos
Flores , Mostardeira , Proteínas de Plantas , Regiões Promotoras Genéticas , Flores/genética , Regulação da Expressão Gênica de Plantas/genética , Mostardeira/enzimologia , Mostardeira/genética , Mutação , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regiões Promotoras Genéticas/genética
13.
Genome Biol ; 21(1): 141, 2020 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-32546280

RESUMO

We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions in addition to base editing. Rice mutants are generated using the SWISS system with efficiencies of cytosine conversion of 25.5%, adenine conversion of 16.4%, indels of 52.7%, and simultaneous triple mutations of 7.3%. The SWISS system provides a powerful tool for multi-functional genome editing in plants.

14.
Sci China Life Sci ; 2020 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-32382982

RESUMO

CRISPR/Cas systems, especially CRISPR/Cas9, generally result in small insertions/deletions, which are unlikely to eliminate the functions of regulatory and other non-coding sequences. To generate larger genomic deletions usually requires the use of pairs of guide RNAs. Here we show that it is possible to create such deletions with a single guide RNA by fusing Cas9 or Cas12a with T5 exonuclease (T5exo). These fusion constructs were found to increase both the frequency and size of deletions at target loci in rice protoplasts and seedlings. Moreover, the genome editing efficiencies of Cas9 and Cas12a were also enhanced by fusion with T5 exonuclease. These T5exo-Cas fusions expand the CRISPR toolbox, and facilitate knockout of regulatory and non-coding DNA sequences. From a wider standpoint, our results suggest a general strategy for producing larger deletions using other Cas nucleases.

15.
Food Chem ; 324: 126887, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32339788

RESUMO

Epigenetic regulation and salt ions play essential roles in senescence control, but the underlying regulatory mechanism of senescence has not been thoroughly revealed in broccoli postharvest buds. Here, we found 200 mmol·L-1 NaCl, 400 mmol·L-1 KCl, 40 mmol·L-1 CaCl2 and 0.5 µmol·L-1 Trichostatin-A (TSA, a histone deacetylase inhibitor) delayed the bud senescence. They resulted in significantly inhibiting the malondialdehyde (MDA) content, and dramatically promoting the contents of superoxide dismutase (SOD), peroxidase (POD) and Chlorophyll. Furthermore, the expression of PHEOPHYTINASE (PPH) and NONYELLOWING (NYE1), but not SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), were remarkably repressed by salt ions and TSA. Interestingly, HISTONE DEACETYLASE 9 (HDA9) and CATION/Ca2+ EXCHANGER 1 (CCX1) were down-regulated by NaCl, CaCl2 and TSA. Further assays demonstrated that HDA9 could not interact with CCX1 promoter. It suggested that CCX1 along with HDA9 were involved in inhibiting the senescence of broccoli buds, and regulated aging by indirect interaction.


Assuntos
Antioxidantes/metabolismo , Brassica/metabolismo , Regulação para Baixo/efeitos dos fármacos , Histona Desacetilases/metabolismo , Proteínas de Plantas/metabolismo , Sais/farmacologia , Sequência de Aminoácidos , Antiporters/química , Antiporters/genética , Antiporters/metabolismo , Brassica/química , Brassica/classificação , Cloreto de Cálcio/química , Cloreto de Cálcio/farmacologia , Clorofila/metabolismo , Flores/química , Flores/metabolismo , Ácidos Hidroxâmicos/química , Ácidos Hidroxâmicos/farmacologia , Íons/química , Filogenia , Sais/química , Alinhamento de Sequência
16.
FASEB J ; 34(5): 7036-7057, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32246808

RESUMO

The purpose was to determine the role of AMPK activation in the renal metabolic response to sepsis, the development of sepsis-induced acute kidney injury (AKI) and on survival. In a prospective experimental study, 167 10- to 12-week-old C57BL/6 mice underwent cecal ligation and puncture (CLP) and human proximal tubule epithelial cells (TEC; HK2) were exposed to inflammatory mix (IM), a combination of lipopolysaccharide (LPS) and high mobility group box 1 (HMGB1). Renal/TEC metabolic fitness was assessed by monitoring the expression of drivers of oxidative phosphorylation (OXPHOS), the rates of utilization of OXPHOS/glycolysis in response to metabolic stress, and mitochondrial function by measuring O2 consumption rates (OCR) and the membrane potential (Δψm ). Sepsis/IM resulted in AKI, increased mortality, and in renal AMPK activation 6-24 hours after CLP/IM. Pharmacologic activation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) or metformin during sepsis improved the survival, while AMPK inhibition with Compound C increased mortality, impaired mitochondrial respiration, decreased OCR, and disrupted TEC metabolic fitness. AMPK-driven protection was associated with increased Sirt 3 expression and restoration of metabolic fitness. Renal AMPK activation in response to sepsis/IM is an adaptive mechanism that protects TEC, organs, and the host by preserving mitochondrial function and metabolic fitness likely through Sirt3 signaling.

17.
Clin Exp Ophthalmol ; 48(5): 682-688, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32173992

RESUMO

BACKGROUND: Geographic atrophy (GA) is a late-stage symptom of an age-related macular degeneration (AMD), characterized by the loss of retinal pigment epithelial (RPE) cells and photoreceptor functions. Despite being a major cause of blindness in individuals of 65 years of age and older, some forms of AMD, including GA, still lack targeted treatment. Our previous study demonstrated that mini-αA peptide, which contains the functional site of αA-crystallin, protected RPE cells from NaIO3 -induced apoptosis. METHODS: To further investigate the underlying mechanism, we applied next-generation sequencing analysis to identify miR-1246 as a putative mediator of mini-αA protective function. To investigate the role of miR-1246 in RPE cell apoptosis, a stable miR-1246-low-expression cell line was established by using miR-1246 inhibitor. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to investigate the proliferation of RPE cells, mRNA and miR-1246 expression were detected by the quantitative reverse transcriptase-polymerase chain reaction. RESULTS: We have further identified caspase-3 and caspase-14 as molecular targets of miR-1246 involved in regulation of apoptosis in NaIO3 -incubated cells. Interestingly, disruption of miR-1246 expression enhanced anti-apoptotic effect of mini-αA on RPE cells during oxidative stress. CONCLUSIONS: Our results provide a mechanistic basis for evaluation of miR-1246 as a new candidate target for the clinical treatment of AMD.

18.
Chemosphere ; 251: 126375, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32151811

RESUMO

Fluorescent dissolved organic matter (FDOM), having complex structures like aromatic structure and double bond structure, is able to represent relatively refractory parts of dissolved organic matter (DOM). This study investigated the distribution of FDOM in the influents and the removal in the secondary effluents of 15 municipal wastewater treatment plants (WWTPs) in 15 provincial capitals of China. Eight components have been identified using excitation emission matrix combined with parallel factor analysis (EEM-PARAFAC). Tryptophan-like (C1 or C4), terrestrial humic-like (C2) and microbial humic-like (C3) fluorescent components were major FDOM components in municipal wastewater, appearing in 11 WWTPs simultaneously. The removal of total fluorescence was generally about 30%-40%, while hydrophobic humic-like compounds (C5 and C8) were the most refractory components with 4%-16% removal and C3 was the second most refractory with -11%-41% removal. The compositions of FDOM in municipal wastewater were different in northeast/west and middle/east regions according to the self-organized map (SOM) analysis. Wastewater sources had more important influence on fluorescent characteristics of secondary effluents than biological treatment processes. Besides, this study found that humification index (HIX) was the most suitable index to describe the bulk fluorescent character of wastewater since it had a good correlation with abundance, removal and ratios of main fluorescent components either in the influents or in the secondary effluents.


Assuntos
Monitoramento Ambiental , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , China , Corantes , Análise Fatorial , Fluorescência , Substâncias Húmicas/análise , Oriente Médio , Espectrometria de Fluorescência , Águas Residuárias/análise
19.
J Ovarian Res ; 13(1): 20, 2020 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-32087757

RESUMO

BACKGROUND: Recent studies have suggested that estrogen (E2) plays an important role in epithelial ovarian cancer (EOC). However, the mechanism of E2 in ovarian cancers is unclear. The purpose of this study was to investigate the effect of E2 on ovarian cancers and illuminate the mechanism of E2 in promote ovarian cancers proliferation. RESULTS: We demonstrated that E2 stimulated the proliferation and invasion of ovarian cancer cells. In this study, ovarian cancer specimens were also analyzed for transient receptor potential channel C3 (TRPC3) expression; TRPC3 expression levels were higher in ovarian cancer samples than in normal ovarian tissue samples. Previous studies have shown that TRPC3 contributes to the progression of human ovarian cancer. In this study, we further investigated the interaction between E2 and TRPC3. We found that E2 stimulation enhanced the expression of TRPC3 at both the mRNA and protein levels. E2 stimulation enhanced the influx of Ca2+. Moreover, siRNA-mediated silencing of TRPC3 expression inhibited the ability of E2 to stimulate the influx of Ca2+. CONCLUSIONS: In conclusion, TRPC3 plays a significant role in the stimulatory activity of E2 and could be a therapeutic target for the treatment of EOC. Furthermore, this study elucidates the molecular mechanism by which E2 promotes the proliferation and migration of EOC cells.

20.
BMC Surg ; 20(1): 30, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32050953

RESUMO

BACKGROUND: Laparoscopic Roux-en-Y gastric bypass (LRYGB) and laparoscopic sleeve gastrectomy (LSG) are two representative bariatric surgeries. This study aimed to compare the effects of the LSG and LRYGB based on high-quality analysis and massive amount of data. METHODS: For this study databases of PubMed, Web of Science, EBSCO, Medline, and Cochrane Library were searched for articles published until January 2019 comparing the outcomes of LSG and LRYGB. RESULTS: This study included 28 articles. Overall, 9038 patients (4597, LSG group; 4441, LRYGB group) were included. The remission rate of type 2 diabetes mellitus (T2DM) in the LRYGB group was superior to that in the LSG group at the 3-years follow-up. Five-year follow-up results showed that LRYGB had an advantage over LSG for the percentage of excess weight loss and remission of T2DM, hypertension, dyslipidemia, and abnormally low-density lipoprotein. CONCLUSIONS: In terms of the long-term effects of bariatric surgery, the effect of LRYGB was better than of LSG.


Assuntos
Gastrectomia/métodos , Derivação Gástrica/métodos , Obesidade Mórbida/cirurgia , Cirurgia Bariátrica/métodos , Diabetes Mellitus Tipo 2/epidemiologia , Humanos , Hipertensão/epidemiologia , Laparoscopia/métodos , Resultado do Tratamento , Perda de Peso
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