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1.
Artigo em Inglês | MEDLINE | ID: mdl-34651265

RESUMO

In this article, dynamic simulation experiments have studied the effects of three capping materials, quartz sand (QS), aluminum-based phosphorus-locking agent (Al-PIA), and lanthanum-modified bentonite (LMB) in reducing phosphorus load in eutrophic water bodies. The changes of various forms of phosphorus in Al-PIA and sediment before and after the test were analyzed, and the mechanism of phosphorus migration and transformation in different capping systems was described. The dynamic simulation test lasted 95 days. The results showed that when the initial concentration of total phosphorus (TP) was 3.55 mg/L, the capping strength was 2 kg/m2 and the hydraulic retention time of water circulation was 0.5 days, indicating that the average reduction rates of TP by LMB, Al-PIA and QS systems were 74.66%, 69.54%, and 3.64%, respectively, compared with the control system. The analysis of variance showed that there were significant differences (P < 0.05) in the TP concentration of the overlying water between the LMB, Al-PIA capping system, and the control system. Lanthanum ions in LMB can fix phosphorus. Al-PIA reduces the phosphorus concentration in water by means of ion exchange, adsorption, complexation, etc. LMB and Al-PIA promoted the migration of phosphorus in sediment. Among them, the phosphorus fixed by Al-PIA was mainly in the form of non-apatite inorganic phosphorus (NAIP) in inorganic phosphorus (IP), which can be seen; Al-PIA can effectively reduce the phosphorus load of eutrophic water.

2.
Biomed Pharmacother ; 143: 112209, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34649343

RESUMO

BACKGROUND: Alveolar hypercoagulation and fibrinolytic inhibition are important characteristics during acute respiratory distress syndrome (ARDS), and NF-κB p65 signaling pathway is involved to regulate these pathophysiologies. We hypothesize that targeting NF-κB signal pathway could ameliorate alveolar hypercoagulation and fibrinolyitc inhibition, thus attenuating lung injury in ARDS. PURPOSE: We explore the efficacy and the potential mechanism of andrographolide sulfonate (Andro-S) on alveolar hypercoagulation and fibrinolytic inhibition in LPS-induced ARDS in mice. METHODS: ARDS was made by lipopolysaccharide (LPS) inhalation in C57BLmice. Andrographolide sulfonate (2.5, 5 and 10 mg/kg) was intraperitoneally given to the mice (once a day for three consecutive days) before LPS administration. NEMO binding domain peptide (NBD), an inhibitor of NF-κB, was used as the positive control and it replaced Andro-S in mice of NBD group. Mice in normal control received saline instead of LPS. Lung tissues and bronchoalveolar lavage fluid (BALF) were collected for analysis of alveolar coagulation, fibrinolytic inhibition as well as of pulmonary inflammatory response after 8 h of LPS inhalation. NF-κB signal pathway in lung tissue was simultaneously determined. RESULTS: Andro-S dose-dependently inhibited tissue factor (TF) and plasminogen activator inhibitor (PAI)-1 expressions either in mRNA or in protein in lung tissue of ARDS mice, and it also decreased the concentrations of TF, PAI-1, thrombin-antithrombin complex (TAT), procollagen peptide type Ⅲ (PⅢP) while promoting the production of activated protein C (APC) in BALF. Meanwhile, Andro-S effectively inhibited inflammatory response (interleukin 1ß and myeloperoxidase) induced by LPS. LPS stimulation dramatically activated NF-κB signal pathway, indicated by increased expressions of phosphorylation of p65 (p-p65), p-IKKα/ß and p-IκBα and the higher p65-DNA binding activity, which were all dose-dependently reversed by Andro-S. Andro-S and NBD presented similar efficacies. CONCLUSIONS: Andro-S treatment improves alveolar hypercoagulation and fibrinolytic inhibition and attenuates pulmonary inflammation in LPS-induced ARDS in mice partly through NF-κB pathway inactivation. The drug is expected to be an effective choice for ARDS.

3.
Nanoscale ; 13(33): 14197-14206, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34477701

RESUMO

Surface modification by loading a water oxidation co-catalyst (WOC) is generally considered an efficient means to optimize the sluggish surface oxygen evolution reaction (OER) of a hematite photoanode for photoelectrochemical (PEC) water oxidation. However, the surface WOC usually exerts little impact on the bulk charge separation of hematite. Herein, an ultrathin citrate-Ni0.9Co0.1(OH)x [Cit-Ni0.9Co0.1(OH)x] is conformally coated on the fluorine-doped hematite (F-Fe2O3) photoanode for PEC water oxidation to simultaneously promote the internal hole extraction and surface hole injection of the target photoanode. Besides, the conformally coated Cit-Ni0.9Co0.1(OH)x overlayer passivates the redundant surface trap states of F-Fe2O3. These factors result in a superior photocurrent density of 2.52 mA cm-2 at 1.23 V versus a reversible hydrogen electrode (V vs. RHE) for the target photoanode. Detailed investigation manifests that the hole extraction property in Cit-Ni0.9Co0.1(OH)x is mainly derived from the Ni sites, while Co incorporation endows the overlayer with more catalytic active sites. This synergistic effect between Ni and Co contributes to a rapid and continuous hole migration pathway from the bulk to the interface of the target photoanode, and then to the electrolyte for water oxidation.

4.
Jpn J Nurs Sci ; : e12449, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34498402

RESUMO

AIM: Nurses are considered a major source of professional support for patients. However, little is known about what cancer patients need from nurses regarding professional support and the provision of services in China. The purpose of this study is to investigate professional nursing supportive care needs, perceived supply, and the possible gaps between the two for patients with cancer in mainland China. METHODS: A total of 390 inpatients with different types of cancer were recruited. The professional nursing supportive care scale was used to assess the needs for and perceived supply of various types of support. RESULTS: The information aspect was one of the most critical needs for supportive care, and the technical aspect was the one with the highest perceived level of supply. Significant mismatches between actual needs and perceived supply were identified. CONCLUSIONS: Significant gaps existed between actual needs and perceived supply. Nurses should pay more attention to the higher actual needs and lower perceived levels of supply regarding supportive care for cancer patients.

5.
Pediatrics ; 148(2)2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34312293

RESUMO

BACKGROUND AND OBJECTIVES: A leading brand of heated tobacco products (HTPs), IQOS, was authorized to be sold in the United States in 2019. Researchers have examined the awareness and use of HTPs among US adults. In this study, we examined high school students' awareness, use, and susceptibility pertaining to HTPs. METHODS: A large, cross-sectional population survey of randomly sampled 10th- and 12th-graders in California (N = 150 516) was conducted online during school hours from September 2019 to March 2020. RESULTS: Overall, 8.9% (95% confidence interval [CI], 8.7%-9.1%) of California high school students had heard of HTPs. Approximately 0.67% (95% CI, 0.61%-0.73%) had ever tried HTPs, and 0.20% (95% CI, 0.17%-0.23%) were current users (ie, ∼30% of ever users continued to use HTPs at the time of survey). Among those who never tried HTPs, 18.3% (95% CI, 17.9%-18.8%) were susceptible to future use. The susceptibility to HTP use was greater among users of cigarettes or e-cigarettes than among nonusers. CONCLUSIONS: The awareness of HTPs among adolescents was remarkable given the low availability of products at the time of survey. Only a small percentage of adolescents experimented with HTPs. However, almost a third of those who had experimented with HTPs continued to use them. This high ratio and the fact that almost 1 out of 5 never users were susceptible to future HTP use should put the public health community on high alert as more HTP products are coming into the market, with promotion of these products likely to increase.

6.
Biomed Pharmacother ; 139: 111569, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34243622

RESUMO

BACKGROUND: Alveolar hypercoagulation and fibrinolysis inhibition were associated with the refractory hypoxemia and the high mortality in patient with acute respiratory distress syndrome (ARDS), and NF-κB pathway was confirmed to contribute to the process. Triptolide (TP) significantly inhibited NF-κB pathway and thus depressed accessive inflammatory response in ARDS. We speculate that TP could improve alveolar hypercoagulation and fibrinolytic inhibition in LPS-induced ARDS via NF-κB inactivation. PURPOSE: The aim of this experiment was to explore the efficacy and potential mechanism of TP on alveolar hypercoagulation and fibrinolysis inhibition in LPS-induced ARDS in mice. METHODS: 50 µl of LPS (5 mg/ml) was inhalationally given to C57BL/6 mice to set up ARDS model. Male mice were randomly accepted with LPS, LPS + TP (1 µg/kg, 10 µg/kg, 50 µg/kg respectively), or with NEMO Binding domain peptide (NBD), an inhibitor of NF-κB. TP (1 µg/kg, 10 µg/kg, 50 µg/kg) were intraperitoneally injected or 10 µg/50 µl of NBD solution were inhaled 30 min before LPS inhalation. A same volume of normal saline (NS) substituted for TP in mice in control. The endpoint of experiment was at 8 hours after LPS stimulation. Pulmonary tissues were taken for hematoxylin-eosin (HE) staining, wet / dry ratio and for lung injury scores (LIS). Tissue factor (TF) and plasminogen activator inhibitor (PAI)-1 in lung tissue were detected by Western-blotting and by quantitative Real-time PCR(qPCR) respectively. Concentrations of TF, PAI-1, thrombin-antithrombin complex (TAT), procollagen peptide type Ⅲ (PⅢP) and activated protein C (APC) in bronchoalveolar lavage fluid (BALF) were measured by ELISA. NF-κB activation and p65-DNA binding activity in pulmonary tissue were simultaneously determined. RESULTS: LPS stimulation resulted in pulmonary edema, neutrophils infiltration, obvious alveolar collapse, interstitial congestion, with high LIS, which were all dose-dependently ameliorated by Triptolide. LPS also dramatically promoted the expressions of TF and PAI-1 either in mRNA or in protein in lung tissue, and significantly stimulated the secretions of TF, PAI-1, TAT, PⅢP but inhibited APC production in BALF, which were all reversed by triptolide treatment in dose-dependent manner. TP dose-dependently inhibited the activation of NF-κB pathway induced by LPS, indicated by the changes of phosphorylations of p65 (p-p65), p-IKKα/ß and p-IκBα, and weakened p65-DNA binding activity. TP and NBD had same efficacies either on alveolar hypercoagulation and fibrinolysis inhibition or on NF-κB signalling pathway in ARDS mice. CONCLUSIONS: TP dose-dependently improves alveolar hypercoagulation and fibrinolysis inhibition in ARDS mice through inhibiting NF-κB signaling pathway. Our data demonstrate that TP is expected to be an effective selection in ARDS.


Assuntos
Diterpenos/farmacologia , Fibrinólise/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Pulmão/efeitos dos fármacos , NF-kappa B/metabolismo , Fenantrenos/farmacologia , Trombofilia/induzido quimicamente , Trombofilia/tratamento farmacológico , Animais , Modelos Animais de Doenças , Compostos de Epóxi/farmacologia , Pulmão/metabolismo , Lesão Pulmonar/tratamento farmacológico , Lesão Pulmonar/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Síndrome do Desconforto Respiratório , Transdução de Sinais/efeitos dos fármacos , Trombofilia/metabolismo , Tromboplastina/metabolismo
7.
J Colloid Interface Sci ; 605: 44-53, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34303923

RESUMO

Palladium (Pd)-based materials have been widely used as catalysts for the methanol oxidation reaction (MOR). Unfortunately, the catalytic activity was limited by structure, carbon monoxide intermediates (COads) tolerance and stability. It was currently difficult to be used in large-scale commercial production. Herein, to further improve their electrocatalytic activity, a facile oxidation method to achieve in-situ oxidation of palladium-iridium (PdIr) alloy on nitrogen-doped graphene (NGS) is used, which is named as Pd-Ir-O/NGS. The new catalyst exhibits remarkable MOR activity (1374.8 mA mg-1), COads tolerance (the onset oxidation potential reach 0.725 V) and stability (the current density retention rate after 500 cycles of cyclic voltammetry is 44.9%). As a catalyst for MOR, the Pd-Ir-O/NGS has more outstanding electrocatalytic performance compared with commercial Pd/C and other counterparts. The mechanism study shows that the excellent catalytic performance is attributed to (1) the synergistic electronic effect of Pd-Ir-O due to the introduction of Ir and O, (2) the insertion of O into PdIr alloy that kinetically accelerated the oxidation of poisoning methoxy intermediates and (3) the vital roles of unique three-dimensional (3D) structure of NGS with abundant nitrogen atoms. Our findings herald a new paradigm for the modification of palladium-based materials for MOR and provide an alternative design principle for novel 3D carbon-based material for various application.

8.
J Colloid Interface Sci ; 604: 198-207, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34265680

RESUMO

Advanced photocatalyst is a key for photocatalytic water purification in the environmental pollutant remediation. In this study, graphitic carbon nitride (g-CN) modified by CuS and TiO2 ternary nanohybrid (CuS-TiO2-g-CN) with close interfacial contact among CuS, TiO2 and g-CN was fabricated through a facile and green method. Compared to the binary g-CN-based counterparts, the CuS-TiO2-g-CN possesses multiple photo-generated charge transfers owing to the synergistic action of CuS, TiO2 and g-CN. And hence the separation efficiency of photo-generated electron-hole pairs can be improved for the CuS-TiO2-g-CN. The optical and photoelectrochemical measurements prove that the CuS-TiO2-g-CN has narrower band gap energy and higher transient photocurrent density than those of g-CN and TiO2-g-CN. Therefore, the CuS-TiO2-g-CN shows notably higher photocatalytic activity and stability towards the degradation of Rhodamine B (RhB) than g-CN and TiO2-g-CN under visible-light irradiation. Moreover, a possible visible-light photocatalytic mechanism of CuS-TiO2-g-CN for degrading RhB was also proposed on the basis of the experimental results and literature reports.


Assuntos
Cobre , Poluentes Ambientais , Catálise , Grafite , Compostos de Nitrogênio , Fotólise , Sulfetos , Titânio
9.
Sci Rep ; 11(1): 12381, 2021 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-34117332

RESUMO

Zoysia japonica is a commonly used turfgrass species around the world. Seed germination is a crucial stage in the plant life cycle and is particularly important for turf establishment and management. Experiments have confirmed that melatonin can be a potential regulator signal in seeds. To determine the effect of exogenous melatonin administration and explore the its potential in regulating seed growth, we studied the concentrations of several hormones and performed a transcriptome analysis of zoysia seeds after the application of melatonin. The total antioxidant capacity determination results showed that melatonin treatment could significantly improve the antioxidant capacity of zoysia seeds. The transcriptome analysis indicated that several of the regulatory pathways were involved in antioxidant activity and hormone activity. The hormones concentrations determination results showed that melatonin treatment contributed to decreased levels of cytokinin, abscisic acid and gibberellin in seeds, but had no significant effect on the secretion of auxin in early stages. Melatonin is able to affect the expression of IAA (indoleacetic acid) response genes. In addition, melatonin influences the other hormones by its synergy with other hormones. Transcriptome research in zoysia is helpful for understanding the regulation of melatonin and mechanisms underlying melatonin-mediated developmental processes in zoysia seeds.

10.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 33(4): 410-415, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-34053482

RESUMO

OBJECTIVE: To investigate the effect of NEMO binding domain peptide (NBDP) on lung inflammation and apoptosis in mice with acute respiratory distress syndrome (ARDS) and its mechanism. METHODS: Thirty-six male BALB/c mice were divided into normal saline (NS) control group, ARDS model group, NBDP negative control group and 6, 12 and 18 µg NBDP pretreatment group by random number table method, with 6 mice in each group. ARDS mouse model was reproduced by aerosol inhalation lipopolysaccharide (LPS) 50 µL. An equivalent among of NS was inhaled in NS control group. The mice in NBDP negative control group were inhaled the materials similar to the non-functional NBDP 30 minutes before the aerosol inhalation LPS; 6, 12 and 18 µg of NBDP 50 µL were respectively inhaled in NBDP pretreatment groups. After inhalation of LPS for 6 hours, mice were sacrificed to get lung tissue and observe the degree of pathological injury and edema. Western blotting was used to detect the phosphorylation of nuclear factor-κB (NF-κB) pathway related proteins [NF-κB inhibitor (IκB) kinaseα/ß(IKKα/ß), IκBα and NF-κB p65; p-IKKα/ß, p-IκBα, p-p65] and the expression of caspase-3 in lung tissue. The bronchoalveolar lavage fluid (BALF) was collected and the levels of inflammatory markers such as myeloperoxidase (MPO), interleukins (IL-1ß, IL-8), and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: ARDS model group had severe edema and hemorrhage, alveolar structure destruction, pulmonary hemorrhage and hyaline membrane formation etc. under light microscope, consistent with the pathological characteristics of ARDS lung tissue, suggesting that the ARDS model was successfully reproduced. ELISA showed that MPO, IL-1ß, IL-8 and TNF-α levels of BALF in ARDS model group were obviously higher than those in NS control group. There were no significant differences in the above inflammatory indicators between NBDP negative control group and ARDS model group. The levels of MPO, IL-1ß, IL-8 and TNF-α in NBDP pretreatment groups were significantly lower than those in ARDS model group in a dose-dependent manner, especially in 18 µg NBDP, the differences were statistically significant as compared with ARDS model group [MPO (ng/L): 393.32±19.35 vs. 985.87±101.50, IL-1ß (ng/L): 43.05±5.11 vs. 97.68±10.88, IL-8 (ng/L): 84.64±2.32 vs. 204.00±17.37, TNF-α (ng/L): 229.13±17.03 vs. 546.73±62.72, all P < 0.05]. Western blotting showed that p-IKKα/ß, p-IκBα, p-p65 and caspase-3 protein expressions in ARDS model group were significantly higher than those in NS control group. There was no significant difference in above NF-κB pathway and apoptosis-related protein expression between the NBDP negative control group and ARDS model group. The p-IKKα/ß, p-IκBα, p-p65 and caspase-3 protein expression in NBDP pretreatment groups were significantly lower than those in ARDS model group in a dose-dependent manner, especially in 18 µg NBDP, the differences were statistically significant as compared with ARDS model group [p-IKKα/ß protein (p-IKKα/ß/ß-actin): 0.15±0.02 vs. 0.42±0.04, p-IκBα protein (p-IκBα/ß-actin): 0.10±0.01 vs. 0.93±0.30, p-p65 protein (p-p65/ß-actin): 0.22±0.05 vs. 1.37±0.21, all P < 0.05]. CONCLUSIONS: NBDP can inhibit inflammatory response and apoptosis in ARDS lung tissue in a dose-dependent manner, and its mechanism is associated with interference NF-κB signaling pathway transduction.


Assuntos
Pneumonia , Síndrome do Desconforto Respiratório , Animais , Apoptose , Peptídeos e Proteínas de Sinalização Intracelular , Pulmão , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peptídeos
11.
Nurse Educ Pract ; 53: 103055, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33951599

RESUMO

Evaluation and cultivation of clinical competence in undergraduate nursing students are essential in nursing education. Self-efficacy and good clinical learning environments are expected to have a positive influence on the clinical competence of undergraduate nursing students, but few studies have focused on the relationships among clinical competence, self-efficacy, and clinical learning environments. This study aimed to examine clinical competence and its association with self-efficacy and clinical learning environments among Chinese undergraduate nursing students. A cross-sectional questionnaire survey was conducted with 1518 undergraduate nursing students being recruited from five medical colleges in China. The nursing students' clinical competence, self-efficacy, and clinical learning environments were assessed using a set of self-reported questionnaires, including Chinese versions of the Holistic Clinical Assessment Tool; General Self-Efficacy Scale; and Clinical Learning Environment, Supervision, and Nurse Teacher Evaluation Scale. Factors significantly associated with the clinical competence of undergraduate nursing students were professional interest, self-efficacy, and clinical learning environments, accounting for 36.1% of the total variance. Self-efficacy played a mediating role between clinical learning environments and clinical competence. The results of this study can be applied as reference for the enhancement of a nursing education program to improve the clinical competence of Chinese undergraduate nursing students.


Assuntos
Bacharelado em Enfermagem , Estudantes de Enfermagem , China , Competência Clínica , Estudos Transversais , Humanos , Autoeficácia , Inquéritos e Questionários
12.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 33(2): 155-160, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33729132

RESUMO

OBJECTIVE: To determine the effect of andrographolide (AD) on the expression of procoagulant and fibrinolytic inhibitory factors in rat type II alveolar epithelial cells (AEC II) stimulated by lipopolysaccharide (LPS). METHODS: The AEC II cells RLE-6TN in the logarithmic growth phase were divided into 5 groups: the normal control (NC) group, the LPS group, and the 6.25, 12.5, and 25 mg/L AD groups (AD 6.25 group, AD 12.5 group, AD 25 group). The NC group was cultured with RPMI 1640 conventional medium. In the LPS group, 5 mg/L LPS was added to the RPMI 1640 conventional medium for stimulation. Cells in the AD groups were treated with 6.25, 12.5, and 25 mg/L AD in advance for 1 hour and then given LPS to stimulate the culture. The cells and cell culture supernatant were collected 24 hours after LPS stimulation. The protein and mRNA expressions of tissue factor (TF), tissue factor pathway inhibitor (TFPI), and plasminogen activator inhibition-1 (PAI-1) in cells were detected by Western blotting and real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). The levels of procollagen III peptide (PIIIP), thrombin-antithrombin complex (TAT), antithrombin III (AT-III) and activated protein C (APC) in the cell supernatant were detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Compared with the NC group, the protein and mRNA expressions of TF and PAI-1 in the LPS group were significantly increased, and the protein and mRNA expressions of TFPI were significantly reduced. At the same time, the levels of PIIIP and TAT in the cell supernatant were significantly increased, the levels of AT-III, APC were significantly reduced. Compared with the LPS group, the protein and mRNA expressions of TF and PAI-1 in AD 6.25 group, AD 12.5 group, AD 25 group were significantly reduced [TF/GAPDH: 0.86±0.08, 0.45±0.04, 0.44±0.04 vs. 1.32±0.10, TF mRNA (2-ΔΔCt): 2.59±0.25, 2.27±0.05, 1.95±0.04 vs. 4.60±0.26, PAI-1/GAPDH: 2.11±0.07, 1.45±0.04, 0.86±0.09 vs. 2.56±0.09, PAI-1 mRNA (2-ΔΔCt): 3.50±0.22, 2.23±0.29, 1.84±0.09 vs. 6.60±0.27, all P < 0.05], while the protein and mRNA expressions of TFPI were significantly increased [TFPI/GAPDH: 0.78±0.05, 0.81±0.03, 0.84±0.07 vs. 0.36±0.02, TFPI mRNA (2-ΔΔCt): 0.46±0.09, 0.69±0.07, 0.91±0.08 vs. 0.44±0.06, all P < 0.05]. Also the levels of PIIIP and TAT in the cell supernatant were significantly reduced, and the levels of AT-III and APC were significantly increased [PIIIP (µg/L): 13.59±0.23, 12.66±0.23, 10.59±0.30 vs. 15.82±0.29, TAT (ng/L): 211.57±6.41, 205.69±4.04, 200.56±9.85 vs. 288.67±9.84, AT-III (µg/L): 102.95±3.86, 123.92±2.63, 128.67±1.67 vs. 92.93±3.36, APC (µg/L): 1 188.95±14.99, 1 366.12±39.93, 1 451.15±29.69 vs. 1 145.55±21.07, all P < 0.05]. With the increase of the dose of AD, the above-mentioned promotion and inhibition effects became more obvious. In the AD 25 group, TF, PAI-1 protein and mRNA expressions decreased, TFPI mRNA expression increased, PIIIP level in the supernatant decreased and AT-III, APC levels increased compared with AD 6.25 group, the difference was statistically significant, and the decrease of PAI-1 protein expression and PIIIP level in the supernatant were also statistically significant compared with AD 12.5 group. CONCLUSIONS: Andrographolide in the dose range of 6.25-25 mg/L can dose-dependently inhibit the expression and secretion of procoagulant and fibrinolytic inhibitor-related factors in AEC II cells RLE-6TN stimulated by LPS, and promote the secretion of anticoagulant factors. 25 mg/L has the most obvious effect.


Assuntos
Células Epiteliais Alveolares , Lipopolissacarídeos , Animais , Diterpenos , Ratos , Terapia Trombolítica , Tromboplastina
13.
Support Care Cancer ; 29(9): 5091-5101, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33598735

RESUMO

PURPOSE: To examine a predictive theoretical model of psychological distress based on the following variables reflected on family caregivers of patients with cancer: the unmet supportive care needs, subjective caregiving burden, social support, and the positive aspects of caregiving. METHODS: A cross-sectional descriptive study was conducted on a sample of 484 dyads of patients and their family caregivers. The caregivers completed structured questionnaires designed to measure psychological distress, unmet supportive care needs, subjective caregiving burden, positive aspects of caregiving, and social support. Patients' demographic variables and medical data were collected from a medical record review. We used a structural equation modeling to test the predictive theoretical model. RESULTS: Path analysis results partially supported the proposed model with satisfactory fit indices. Specifically, family caregivers with an increasing number of unmet needs or a heavier caregiving burden were more likely to have more severe psychological distress. Bootstrapping results supported that the caregiving burden and social support were significant mediators. Greater unmet supportive care needs predicted higher psychological distress through increasing caregiving burden. Stronger social support predicted lower psychological distress through decreasing caregiving burden. Positive aspects of caregiving predicted lower caregiving burden through the increasing perceived social support, which in turn eliminated psychological distress. CONCLUSIONS: Unmet supportive care needs could cause psychological distress through increasing caregiving burden. The positive aspects of caregiving reduced caregiving burden through increasing social support, which subsequently alleviated psychological distress. Interventions that aim to satisfy supportive care needs, to reduce caregiving burden, and to strengthen social support ties may boost the mental health of family caregivers.


Assuntos
Cuidadores , Neoplasias , Angústia Psicológica , Efeitos Psicossociais da Doença , Estudos Transversais , Família , Humanos , Neoplasias/terapia , Apoio Social , Estresse Psicológico/epidemiologia , Inquéritos e Questionários
14.
Medicine (Baltimore) ; 100(4): e24533, 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33530282

RESUMO

BACKGROUND: Evidence suggesting a role for including calcineurin inhibitors(CNIs) in early therapy remains limited for low quality and mainly based on small observation cohort study. We will conduct a systematic reviews to explore the effect and adverse effect of calcineurin inhibitors compared with other interventions in the treatment of primary focal segmental glomerulosclerosis (FSGS). METHODS: A comprehensive literature search of MEDLINE (through PubMed), EMBASE, The Cochrane Library, Cochrane Central Register of Controlled Trials (CENTRAL) will be conducted. Two investigators will independently select studies, extract data and assess the quality of the included study. Extracted information will include study characteristics, the contents of included randomized controlled trials, outcomes, the quality of randomized controlled trials and etc. A risk of bias tool will be used to assess the methodological quality. Any disagreement will be resolved by the third investigator. There is no requirement of ethical approval and informed consent. RESULTS: This study will provide high-quality evidence for treatment of FSGS in terms of effectiveness and safety. CONCLUSION: This systematic review aims to provide evidence for treatment of FSGS in different CNIs. REGISTRATION: The systematic review and meta-analysis is registered in the OSF REGISTERS (10.17605/OSF.IO/3B7DE) international prospective register of systematic review.


Assuntos
Inibidores de Calcineurina/uso terapêutico , Glomerulosclerose Segmentar e Focal/tratamento farmacológico , Humanos , Metanálise como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Revisões Sistemáticas como Assunto
15.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 33(1): 53-58, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33565401

RESUMO

OBJECTIVE: To observe the effects of berberine on procoagulant and fibrinolytic inhibitory factors produced by rat type II alveolar epithelial cell (AEC II) induced by lipopolysaccharide (LPS). METHODS: AEC II cells (RLE-6TN cells) were cultured in vitro, and the cells in logarithmic growth phase were collected. The cytotoxicity text of berberine was detected by cell counting kit-8 (CCK-8) to determine the drug concentration range according to inhibition concentration of half cells (IC50). The RLE-6TN cells were divided into five groups, the cells in blank control group were cultured in DMEM; the cells in LPS group were stimulated with 5 mg/L LPS; and the cells in berberine pretreatment groups were pretreated with 20, 50 and 80 µmol/L berberine for 1 hour, and then were co-cultured with 5 mg/L LPS. The cells were collected after LPS induced for 24 hours. The protein and mRNA expression levels of tissue factor (TF), tissue factor pathway inhibitor (TFPI) and plasminogen activator inhibitor-1 (PAI-1) in the cells were detected by Western blotting and real-time fluorescence quantification reverse transcription-polymerase chain reaction (RT-qPCR). The levels of activated protein C (APC), precollagen III peptide (PIIIP), thrombin-antithrombin complex (TAT) and antithrombin III (AT III) in the cell supernatant were measured by enzyme linked immunosorbent assay (ELISA). RESULTS: According to the inhibition rate curve, the IC50 of berberine on RLE-6TN cells was 81.16 µmol/L. Therefore, 20, 50 and 80 µmol/L were selected as the intervention concentration of berberine. Compared with the blank control group, the expression and secretion of procoagulant and fibrinolytic inhibitory factors were abnormal in RLE-6TN cells after LPS induced for 24 hours. The protein and mRNA expression levels of TF and PAI-1 in the LPS group were significantly increased, but the protein and mRNA expression levels of TFPI were significantly decreased. Meanwhile, the levels of APC and AT III in the cell supernatant were significantly decreased, while the levels of PIIIP and TAT were significantly increased. After pretreatment with berberine, the abnormal expression and secretion of procoagulant and fibrinolytic inhibitory factors induced by LPS were corrected in a dose-dependent manner, especially in 80 µmol/L. Compared with the LPS group, the protein and mRNA expression levels of TF and PAI-1 in the berberine 80 µmol/L group were significantly decreased [TF protein (TF/GAPDH): 0.45±0.02 vs. 0.55±0.03, TF mRNA (2-ΔΔCt): 0.39±0.08 vs. 1.48±0.11, PAI-1 protein (PAI-1/GAPDH): 0.37±0.02 vs. 0.64±0.04, PAI-1 mRNA (2-ΔΔCt): 1.14±0.29 vs. 4.18±0.44, all P < 0.01] and those of TFPI were significantly increased [TFPI protein (TFPI/GAPDH): 0.53±0.02 vs. 0.45±0.02, TFPI mRNA (2-ΔΔCt): 0.94±0.08 vs. 0.40±0.05, both P < 0.01]. Meanwhile, the levels of APC and AT III in the cell supernatant were significantly increased [APC (µg/L): 1 358.5±26.0 vs. 994.2±23.1, AT III (µg/L): 118.0±7.4 vs. 84.4±2.7, both P < 0.01], while those of PIIIP and TAT were significantly decreased [PIIIP (µg/L): 11.2±0.4 vs. 18.6±0.9, TAT (ng/L): 222.1±2.8 vs. 287.6±7.0, both P < 0.01]. CONCLUSIONS: Berberine could inhibit the LPS-induced expressions of procoagulant and fibrinolytic inhibitory factors in rat AEC II cells and promote the expressions of anticoagulant factors in a dose-dependent manner. Berberine may be a new therapeutic target for alveolar hypercoagulability and fibrinolysis inhibition in acute respiratory distress syndrome (ARDS).


Assuntos
Berberina , Lipopolissacarídeos , Células Epiteliais Alveolares , Animais , Berberina/farmacologia , Fibrinólise , Ratos , Tromboplastina
16.
Environ Sci Pollut Res Int ; 28(14): 18062-18069, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33410060

RESUMO

It is well-known that the activated thin-layer capping covering by secondary capping of contaminated sediment poses a threat to the inactivation of activated material. In this study, the static simulation experiment was conducted to study the effect of secondary capping thickness by sediment on the control of TP release from the sediment by aluminum-based P-inactivation agent (Al-PIA), and to propose the phosphorus adsorption pathway of Al-PIA. The results showed that Al-PIA could effectively reduce the release of phosphorus pollutants from the sediment at the capping intensity of 2 kg/m2. When the secondary capping thickness of sediment were 0, 2, 4, 7, 10, and 15 mm, the average removal rates of TP were 87.57%, 76.39%, 61.22%, 51.32%, 41.93%, and 32.11%, respectively, indicating that the removal efficiency of phosphorus decreased with the increase of the secondary capping thickness of the sediment. The adsorbed phosphorus by Al-PIA was mainly non-apatite inorganic phosphorus (NAIP) in inorganic phosphorus. With the increase of the secondary capping thickness of sediment, the NAIP proportion of phosphorus adsorbed by Al-PIA increased. Meanwhile, the removal rate of phosphorus in the activated capping system showed a first increase and then decrease trend, and the removal rates of total phosphorus (TP), inorganic phosphorus (IP), and organic phosphorus (OP) were obvious except for that of organic phosphorus (OP).


Assuntos
Fósforo , Poluentes Químicos da Água , Adsorção , Alumínio , Sedimentos Geológicos , Poluentes Químicos da Água/análise
17.
Bioorg Med Chem ; 29: 115856, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-33199201

RESUMO

NIK is a critical regulatory protein of the non-classical NF-kB pathway, and its dysregulated activation has been proved to be one of the pathogenic factors in a variety of autoimmune diseases and inflammatory diseases. Nevertheless, its corresponding development of inhibitors faces many obstacles, including the lack of structure types of known inhibitors, immature activity evaluation methods of compounds in vitro. In this study, a series of quinoline derivatives were obtained through rational design and chemical synthesis. Among them, the representative compounds 17c and 24c have excellent inhibitory activities on LPS-induced macrophage (J774) nitric oxide release and anti-Con A-stimulated primary T cell proliferation. This evaluation method has good universality and overcomes the obstacles mentioned above, which are faced by the current inhibitor research to a certain extent. Besides, the compound's toxicity against the growth of T cells under non-stress conditions was evaluated, for the first time, as an indicator for the investigation to avoid potential safety risks. Pharmacokinetic properties evaluation of the less toxic compound 24c confirmed its good metabolic behavior (especially oral properties, F% = 21.7%), and subsequent development value.

18.
Int Immunopharmacol ; 88: 107020, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33182048

RESUMO

BACKGROUND: Alveolar hypercoagulation and pulmonary inflammation are important characteristics and they regulate each other in acute respiratory distress syndrome (ARDS). NF-κB pathway has been confirmed to be involved in regulation of this crosstalk. Emodin, a traditional Chinese herb, shows potent inhibitory effect on NF-κB pathway, but whether it is effective in alveolar hypercoagulation and pulmonary inflammation in ARDS remains to be elucidated. PURPOSE: The aim of this experiment was to evaluate the efficacy of emodin on LPS-provoked alveolar hypercoagulation and excessive pulmonary inflammation in ARDS, and its potential mechanism. METHODS: Mice ARDS was set up through LPS (40 µl, 4 mg/ml) inhalation. Male mice were randomly received with BPS, LPS only, LPS+ emodin (5 mg/kg, 10 mg/kg, 20 mg/kg, respectively) and BAY65-1942, an inhibitor of IKKß. After 48 h of LPS stimulation, pulmonary pathological injury, expressions of Tissue factor (TF), plasminogen activator inhibitor (PAI)-1, activated protein C (APC), collagen Ⅰ, collagen III, interleukin (IL) 8, IL-1ß and tumor necrosis factor (TNF)-α in lung tissues, as well as concentrations of antithrombin III (AT III), procollagen peptide type III (PIIIP), soluble thrombomodulin (sTM), thrombin antithrombin complex (TAT), myeloperoxidase (MPO) and the percentage of inflammatory cells in bronchoalveolar lavage fluid (BALF) were all determined. NF-κB pathway activation as well as NF-κB DNA binding activity in pulmonary tissue were simultaneously checked. RESULTS: LPS stimulation resulted in obvious lung injury, excessive inflammatory cells infiltration, which all were dose-dependently ameliorated by emodin. Expressions of TF, PAI-1, collagen Ⅰ and collagen III as well as IL-8, IL-1ß and TNF-α in pulmonary tissue were all elevated while APC decreased under LPS provocation, which were all reversed by emodin treatment in dose-dependent manner. LPS promoted the secretions of PIIIP, sTM, TAT and inhibited AT III production in BALF, and resulted in high levels of MPO and the percentage of inflammatory cells in BALF, all of which were significantly and dose-dependently attenuated while AT III production was increased by emodin. Meanwhile, emodin effectively inhibited NF-κB pathway activation and attenuated p65 DNA binding activity induced by LPS inhalation. Emodin and BAY-65-1942 had similar impacts in this experiment. CONCLUSIONS: Emodin improves alveolar hypercoagulation and fibrinolytic inhibition and depresses excessive pulmonary inflammation in ARDS mice in dose-dependent manner via NF-κB inactivation. Our data demonstrate that emodin is expected to be an effective drug in alveolar hypercoagulation and pulmonary inflammation in ARDS.


Assuntos
Emodina/uso terapêutico , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Lipopolissacarídeos/toxicidade , NF-kappa B/metabolismo , Síndrome do Desconforto Respiratório/induzido quimicamente , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Camundongos , NF-kappa B/genética , Alvéolos Pulmonares/efeitos dos fármacos , Alvéolos Pulmonares/patologia , Síndrome do Desconforto Respiratório/tratamento farmacológico , Trombofilia/induzido quimicamente , Trombofilia/tratamento farmacológico
19.
J Clin Nurs ; 29(23-24): 4469-4481, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32949056

RESUMO

OBJECTIVE: To systematically review male partners' caring experience and supportive care needs when caring for women with gynaecologic cancer. METHODS: The PRISMA guidelines were used to conduct this systematic review. We performed a comprehensive literature search in nine databases and qualitative studies published in English or Chinese from inception to January 2020. The included papers were appraised, using the Critical Appraisal Skills Program tool for qualitative research. An inductive thematic analysis method was adopted to synthesise major findings to construct core concepts and themes. RESULTS: Eight studies were included in this review, and four overarching themes emerged the following: the negative experience of disease, the need for supportive care to cope, adapting to a new life and post-traumatic growth. CONCLUSIONS: This study shows that male partners had both negative and positive experiences in the caring process, and they could adjust themselves to some extent. Their perceived supportive care needs were often neglected. RELEVANCE TO CLINICAL PRACTICE: Male partners of women with gynaecologic cancer are an under-recognised group. The couple-oriented or family-oriented supportive care programmes should be implemented to meet the supportive care needs of male partners to enhance their health and well-being.


Assuntos
Neoplasias dos Genitais Femininos , Adaptação Psicológica , Feminino , Humanos , Masculino , Pesquisa Qualitativa
20.
Nanoscale ; 12(28): 15193-15200, 2020 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-32638787

RESUMO

The photoelectrochemical (PEC) water reduction performance of CuBi2O4 (CBO)-based photocathodes is still far from their theoretical values due to low bulk and surface charge separation efficiencies. Herein, we propose a regrowth strategy to prepare a photocathode with CBO coating on Zn-doped CBO (CBO/Zn-CBO). Furthermore, NaBH4 treatment of CBO/Zn-CBO introduced oxygen vacancies (Ov) on CBO/Zn-CBO. It was found that Zn-doping not only increases the charge carrier concentration of CBO, but also leads to appropriate band alignment to form homojunctions. This homojunction can effectively promote the separation of electron-hole pairs, thus obtaining excellent photocurrent density (0.5 mA cm-2 at 0.3 V vs. RHE) and charge separation efficiency (1.5 times than CBO). The following surface treatment induced Ov on CBO/Zn-CBO, which significantly increased the active area of the surface catalytic reaction and further enhanced the photocurrent density (0.6 mA cm-2). In the absence of cocatalysts, the electron injection efficiency of Ov/CBO/Zn-CBO was 1.47 times improved than that of CBO. This work demonstrates a homojunction photocathode with Ov modulation, which provides a new view for future photoelectrochemical water splitting.

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