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1.
Zhongguo Zhen Jiu ; 42(3): 321-4, 2022 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-35272413

RESUMO

The paper introduced the experiences of quality management in the implementation of multi-center acupuncture clinical trials and the keys in training acupuncture operators. The process management was explained in view of the division of labor for researchers, protocol learning and the communication among sub-centers. Besides, specificity links of acupuncture research were summarized, i.e. meaning implementation brief of acupuncture operation training, control for quantity of stimulus in acupuncture and doctor-patient communication. It is anticipated to provide a valuable reference for the quality control and improvement of multi-center acupuncture clinical trial in future.


Assuntos
Terapia por Acupuntura , Ensaios Clínicos como Assunto , Estudos Multicêntricos como Assunto , Humanos , Controle de Qualidade
2.
Front Cardiovasc Med ; 8: 773314, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34957257

RESUMO

Various stresses, including pressure overload and myocardial stretch, can trigger cardiac remodeling and result in heart diseases. The disorders are associated with high risk of morbidity and mortality and are among the major health problems in the world. MicroRNAs, a class of ~22nt-long small non-coding RNAs, have been found to participate in regulating heart development and function. One of them, miR-208a, a cardiac-specific microRNA, plays key role(s) in modulating gene expression in the heart, and is involved in a broad array of processes in cardiac pathogenesis. Genetic deletion or pharmacological inhibition of miR-208a in rodents attenuated stress-induced cardiac hypertrophy and remodeling. Transgenic expression of miR-208a in the heart was sufficient to cause hypertrophic growth of cardiomyocytes. miR-208a is also a key regulator of cardiac conduction system, either deletion or transgenic expression of miR-208a disturbed heart electrophysiology and could induce arrhythmias. In addition, miR-208a appeared to assist in regulating the expression of fast- and slow-twitch myofiber genes in the heart. Notably, this heart-specific miRNA could also modulate the "endocrine" function of cardiac muscle and govern the systemic energy homeostasis in the whole body. Despite of the critical roles, the underlying regulatory networks involving miR-208a are still elusive. Here, we summarize the progress made in understanding the function and mechanisms of this important miRNA in the heart, and propose several topics to be resolved as well as the hypothetical answers. We speculate that miR-208a may play diverse and even opposite roles by being involved in distinct molecular networks depending on the contexts. A deeper understanding of the precise mechanisms of its action under the conditions of cardiac homeostasis and diseases is needed. The clinical implications of miR-208a are also discussed.

3.
Opt Express ; 29(16): 25189-25201, 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34614855

RESUMO

Recently, ultrathin localized spoof surface plasmon (LSSP) resonators are found to have intrinsic defects of relatively low quality factors (Q-factors) because of unavoidable material and radiation losses. In this paper, multilayer structures of planar-circular-grating resonators and their magnetic-coupling schemes are proposed to achieve effective excitation of high-Q LSSPs modes. By adopting the multilayer structures with air between the layers, the power dissipation effected by both material and radiation losses is significantly suppressed. Experimental results show that the Q-factors could reach more than 200 and the excitation efficiencies could reach more than 90%. Numerical simulations show the distribution of the electromagnetic field and illustrate the principle of magnetic coupling. Besides, the Q-factors of resonators with different structural parameters were measured and analyzed. This study aims to provide some inspirations on planar gyro-devices and to improve the performance of existing applications, such as sensors and filters.

4.
Bioorg Chem ; 115: 105178, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34303897

RESUMO

Four undescribed ent-kaurane diterpenoids, wilkaunoids A - D (1-4), and three undescribed abietane diterpenoids, wilabinoids A - C (13-15), along with thirteen known ones (5-12 and 16-20), were isolated from Tripterygium wilfordii. Their structures were elucidated by extensive spectroscopic methods, electroniccirculardichroism calculation, and X-ray diffraction analysis. Compounds 1 and 2 were a pair of C-19 epimers of ent-kaurane diterpenoids, featuring a rare 19,20-epoxy-19,20-dimethoxy-kaurane fragment. Compound 3 possessed a rare naturally occurring 1,3-dioxacyclohexane moiety. Compounds 13 and 15 represented the first example of abietane diterpenoids with an isovalerate substitution from the genus of Tripterygium. The possible biosynthetic pathways of 1-3 were postulated. The effect of 1-20 on nitric oxide production was examined in lipopolysaccharide-stimulated RAW 264.7 cells. Abietane diterpenoid quinones 7-13 (IC50: 1.9-10.2 µM) exhibited the significant activity to inhibit nitric oxide production versus positive control (NG-monomethyl-l-arginine acetate salt, IC50 = 24.9 µM). The structure activity relationship of 7-13 in inhibiting nitric oxide production was then discussed. The most potent 7 and 8 were found to significantly suppress the expression of cyclooxygenase-2 and inducible nitric oxide synthase proteins, showing a good anti-inflammatory potential. The findings provided some valuable insights for the discovery and structural modification of abietane diterpenoids towards anti-inflammatory lead compounds.


Assuntos
Abietanos/farmacologia , Anti-Inflamatórios/farmacologia , Diterpenos do Tipo Caurano/farmacologia , Tripterygium/química , Abietanos/química , Animais , Anti-Inflamatórios/química , Diterpenos do Tipo Caurano/química , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Células RAW 264.7
5.
Infect Dis Poverty ; 10(1): 62, 2021 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-33962683

RESUMO

BACKGROUND: A local coronavirus disease 2019 (COVID-19) case confirmed on June 11, 2020 triggered an outbreak in Beijing, China after 56 consecutive days without a newly confirmed case. Non-pharmaceutical interventions (NPIs) were used to contain the source in Xinfadi (XFD) market. To rapidly control the outbreak, both traditional and newly introduced NPIs including large-scale management of high-risk populations and expanded severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) PCR-based screening in the general population were conducted in Beijing. We aimed to assess the effectiveness of the response to the COVID-19 outbreak in Beijing's XFD market and inform future response efforts of resurgence across regions. METHODS: A modified susceptible-exposed-infectious-recovered (SEIR) model was developed and applied to evaluate a range of different scenarios from the public health perspective. Two outcomes were measured: magnitude of transmission (i.e., number of cases in the outbreak) and endpoint of transmission (i.e., date of containment). The outcomes of scenario evaluations were presented relative to the reality case (i.e., 368 cases in 34 days) with 95% Confidence Interval (CI). RESULTS: Our results indicated that a 3 to 14 day delay in the identification of XFD as the infection source and initiation of NPIs would have caused a 3 to 28-fold increase in total case number (31-77 day delay in containment). A failure to implement the quarantine scheme employed in the XFD outbreak for defined key population would have caused a fivefold greater number of cases (73 day delay in containment). Similarly, failure to implement the quarantine plan executed in the XFD outbreak for close contacts would have caused twofold greater transmission (44 day delay in containment). Finally, failure to implement expanded nucleic acid screening in the general population would have yielded 1.6-fold greater transmission and a 32 day delay to containment. CONCLUSIONS: This study informs new evidence that in form the selection of NPI to use as countermeasures in response to a COVID-19 outbreak and optimal timing of their implementation. The evidence provided by this study should inform responses to future outbreaks of COVID-19 and future infectious disease outbreak preparedness efforts in China and elsewhere.


Assuntos
COVID-19/epidemiologia , Pequim/epidemiologia , COVID-19/transmissão , Teste para COVID-19 , China/epidemiologia , Monitoramento Epidemiológico , Humanos , Modelos Estatísticos , Pandemias , Quarentena , SARS-CoV-2/isolamento & purificação
6.
Zhongguo Zhen Jiu ; 41(1): 94-8, 2021 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-33559451

RESUMO

The employment situation and advantages of interdisciplinary talents of English and acupuncture-moxibustion and tuina at home and abroad are analyzed. The employment rate and further education rate of interdisciplinary talents of English and acupunctue-moxibustion and tuina are higher than those of the normal undergraduates majoring in acupunctue-moxibustion and tuina. The interdisciplinary talents of English and acupuncture-moxibustion and tuina at home are characterized by solid professional foundation and good specialized English, and high comprehensive quality; and the interdisciplinary talents at abroad are characterized by solid professional foundation and good language ability. The interdisciplinary talents of English and acupuncture-moxibustion and tuina should grasp the advantages, cultivate international vision to adapt to international standards, improve the level of clinical practice, promote comprehensive ability, furthermore, improve the employment competitiveness and promote the internationalization of acupuncture- moxibustion and tuina.


Assuntos
Terapia por Acupuntura , Acupuntura , Moxibustão , Emprego , Idioma
7.
Gene ; 772: 145349, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33338511

RESUMO

Inverted cuttings of Populus yunnanensis are characterized by enlarged stems and dwarfed new shoots, and phytohormones play a crucial role in the response to inversion. The polar auxin transport (PAT) system is distinct from the transport systems of other hormones and is controlled by three major transporter gene families: pin-formed (PIN), auxin-resistant/like aux (AUX/LAX) and ATP-binding cassette transporters of the B class (ABCB). Here, we identified these three families in P. trichocarpa, P. euphratica and P. yunnanensis through a genome-wide analysis. The Populus PIN, AUX/LAX and ABCB gene families comprised 15, 8 and 31 members, respectively. Most PAT genes in Populus and Arabidopsis were identified as clear sister pairs, and some had unique motifs. Transcriptome profiling revealed that the expression of most PAT genes was unrelated to cutting inversion and that only several genes showed altered expression when cuttings were inverted. The auxin content difference at positions was opposite in upright and inverted cutting bodies during rooting, which obeyed the original plant polarity. However, during plant growth, the two direction types exhibited similar auxin movements in the cutting bodies, and the opposite auxin changes were observed in new shoots. Four PAT genes with a positive response to cutting inversion, PyuPIN10, PyuPIN11, PyuLAX6 and PyuABCB27, showed diverse expression patterns between upright and inverted cuttings during rooting and plant growth. Furthermore, PAT gene expression retained its polarity, which differs from the results found for auxin flow during plant growth. The inconformity indicated that a new downward auxin flow in addition to the old upward flow might be established during the growth of inverted cuttings. Some highly polar PAT genes were involved in the maintenance of original auxin polarity, which might cause the enlarged stems of inverted cuttings. This work lays a foundation for understanding the roles of auxin transport in plant responses to inversion.


Assuntos
Perfilação da Expressão Gênica/métodos , Ácidos Indolacéticos/metabolismo , Proteínas de Membrana Transportadoras/genética , Populus/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Proteínas de Membrana Transportadoras/metabolismo , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Populus/classificação , Populus/genética , Populus/metabolismo , Sequenciamento Completo do Genoma
8.
Expert Rev Anticancer Ther ; 20(6): 453-464, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32459519

RESUMO

INTRODUCTION: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains a curable strategy for acute lymphoblastic leukemia (ALL), especially for adult cases. However, leukemia relapse after allograft restricts the improvement of transplant outcomes. Measurable residual disease (MRD) has been the strongest predictor for relapse after allo-HSCT, allowing MRD-directed preemptive therapy. AREAS COVERED: This manuscript summarizes the detection of MRD in patients with ALL who undergo allo-HSCT, focusing the effects of positive pre-HSCT MRD and post-HSCT MRD on outcomes as well as MRD-directed interventions. EXPERT OPINION: Except for MFC and RQ-PCR, other strategies, such as next-generation sequencing and RNAseq, have been developed for MRD determination. Negative effects of positive MRD peri-transplantation on outcomes of ALL patients were observed both in human leukocyte antigen (HLA)-matched sibling donor transplantation and in alternative donor transplantation. Advances have been made in determining the need for transplant according to MRD evaluation after induction or consolidation therapy. A number of approaches, including CAR-T-cell therapy, antibodies (blinatumomab, etc), targeted therapy (imatinib, etc), transplant donor selection, as well as donor lymphocyte infusion and interferon-α, have been successfully used or are promising for peri-transplantation MRD interventions. This progress could lead to the significant improvement of transplant outcomes for ALL patients.


Assuntos
Transplante de Células-Tronco Hematopoéticas/métodos , Neoplasia Residual , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Adulto , Aloenxertos , Seleção do Doador , Humanos , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Recidiva , Transplante Homólogo , Resultado do Tratamento
9.
Front Oncol ; 10: 320, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32257948

RESUMO

Background: This study compared the effects of pre-transplantation measurable residual disease (pre-MRD) on outcomes in Philadelphia chromosome (Ph)-positive ALL patients who underwent human leukocyte antigen-matched sibling donor transplantation (MSDT) or who received unmanipulated haploidentical SCT (haplo-SCT). Methods: A retrospective study (n = 202) was performed. MRD was detected by RT-PCR and multiparameter flow cytometry. Results: In the total patient group, patients with positive pre-MRD had a higher 4-year cumulative incidence of relapse (CIR) than that in patients with negative pre-MRD (26.1% vs. 12.1%, P = 0.009); however, the cumulative incidence of non-relapse mortality (NRM) (7.4% vs. 15.9%, P = 0.148), probability of leukemia-free survival (LFS) (66.3% vs. 71.4%, P = 0.480), and overall survival (OS) (68.8% vs. 76.5%, P = 0.322) were comparable. In the MSDT group, patients with positive pre-MRD had increased 4-year CIR (56.4% vs. 13.8%, P < 0.001) and decreased 4-year LFS (35.9% vs. 71.0%, P = 0.024) and OS (35.9% vs. 77.6%, P = 0.011) compared with those with negative pre-MRD. In haplo-SCT settings, the 4-year CIR (14.8% vs. 10.7%, P = 0.297), NRM (7.3% vs. 16.3%, P = 0.187) and the 4-year probability of OS (77.7% vs. 72.3%, P = 0.804) and LFS (80.5% vs. 75.7%, P = 0.660) were comparable between pre-MRD positive and negative groups. In subgroup patients with positive pre-MRD, haplo-SCT had a lower 4-year CIR (14.8% vs. 56.4%, P = 0.021) and a higher 4-year LFS (77.7% vs. 35.9%, P = 0.036) and OS (80.5% vs. 35.9%, P = 0.027) than those of MSDT. Multivariate analysis showed that haplo-SCT was associated with lower CIR (HR, 0.288; P = 0.031), superior LFS (HR, 0.283; P = 0.019) and OS (HR, 0.252; P = 0.013) in cases with a positive pre-MRD subgroup. Conclusions: Our results indicate that the effects of positive pre-MRD on the outcomes of patients with Ph-positive ALL are different according to transplant modality. For Ph-positive cases with positive pre-MRD, haplo-SCT might have strong graft-vs.-leukemia (GVL) effects.

10.
Vet Med Sci ; 6(3): 491-499, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32124566

RESUMO

This study was conducted to evaluate the effects of dietary sodium butyrate (SB) supplementation on growth performance, the development of gastrointestinal tract and immune organs (thymus, spleen and bursa of fabricius), and serum antibody titer after Newcastle disease (ND) vaccination in broilers. The total of 288 1-day-old broilers were randomly allocated to four groups with six replications according to initial body weight. Four treatment groups were designed as follows and fed the indicated diets: CON, basal diet; T1, basal diet supplemented with 0.3 g/kg SB; T2, basal diet supplemented with 0.6 g/kg SB; T3, basal diet supplemented with 1.2 g/kg SB. During days 1-21, broilers fed the T2 diet had higher (p < .05) average daily gain (ADG) than broilers fed the CON diet. On day 21, dietary SB supplementation showed linear increase (p < .05) in relative weight of the duodenum, jejunum, ileum, small intestine (the sum weight of duodenum, jejunum and ileum), pancreas and thymus, and linear increase (p < .05) in relative length of the duodenum, jejunum, ileum, small intestine (the sum length of duodenum, jejunum and ileum) and caeca. Meanwhile, dietary SB supplementation showed linear increase in the antibody titer against ND on days 14, 21, 28 and 35. In conclusion, dietary SB supplementation improved the development of gastrointestinal by increasing the relative weight and length, as well as enhanced the immune response of ND vaccine.


Assuntos
Ácido Butírico/metabolismo , Galinhas/fisiologia , Trato Gastrointestinal/crescimento & desenvolvimento , Sistema Imunitário/crescimento & desenvolvimento , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal/efeitos dos fármacos , Animais , Ácido Butírico/administração & dosagem , Galinhas/crescimento & desenvolvimento , Dieta/veterinária , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Feminino , Trato Gastrointestinal/efeitos dos fármacos , Sistema Imunitário/efeitos dos fármacos , Masculino , Distribuição Aleatória
11.
Transplant Proc ; 52(1): 345-352, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31918969

RESUMO

BACKGROUND AND OBJECTIVE: The aim of this study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on the proliferation and apoptosis of bone marrow (BM) B cells from healthy donors and its mechanism. MATERIALS AND METHODS: The proliferation ability and apoptosis of BM cells from healthy donors before and after in vivo G-CSF application were determined by multiparameter flow cytometry. The gene expression of B cells was detected by RNA-Seq. In vitro experiments were performed to investigate the effects of G-CSF on the proliferation and apoptosis of BM B cells through which gene. RESULTS: Treating healthy donors with G-CSF significantly decreased proliferation and increased apoptosis of BM B cells. The proliferation of CD19+CD27- B cell subgroup and CD19+CD24hiCD38hi B cell subset were also decreased. G-CSF also significantly altered proapoptotic genes, cell cycle arrest genes, and DNA replication and cell cycle genes, especially significantly increased SOCS1 expression of BM B cells. In vitro experiments showed that SOCS1 overexpression did not affect B cell proliferation ability and apoptosis. CONCLUSIONS: Our results suggest that extensive effects of G-CSF on BM B cells, such as inhibiting proliferation, inducing apoptosis, and altering a series of gene expression.


Assuntos
Apoptose/efeitos dos fármacos , Linfócitos B/efeitos dos fármacos , Células da Medula Óssea/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fator Estimulador de Colônias de Granulócitos/farmacologia , Adolescente , Adulto , Idoso , Transplante de Medula Óssea/métodos , Feminino , Expressão Gênica/efeitos dos fármacos , Humanos , Masculino , Pessoa de Meia-Idade , Proteína 1 Supressora da Sinalização de Citocina/biossíntese , Proteína 1 Supressora da Sinalização de Citocina/efeitos dos fármacos , Doadores de Tecidos , Adulto Jovem
12.
Nanoscale Res Lett ; 14(1): 337, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686268

RESUMO

A suspended monolayer graphene has only about 2.3% absorption rate in visible and infrared band, which limits its optoelectronic applications. To significantly increase graphene's absorption efficiency, a tunable dual-band and polarization-insensitive coherent perfect absorber (CPA) is proposed in the mid-infrared regime, which contains the silicon array coupled in double-layers graphene waveguide. Based on the FDTD methods, dual-band perfect absorption peaks are achieved in 9611 nm and 9924 nm, respectively. Moreover, due to its center symmetric feature, the proposed absorber also demonstrates polarization-insensitive. Meanwhile, the coherent absorption peaks can be all-optically modulated by altering the relative phase between two reverse incident lights. Furthermore, by manipulating the Fermi energies of two graphene layers, two coherent absorption peaks can move over a wide spectrum range, and our designed CPA can also be changed from dual-band CPA to narrowband CPA. Thus, our results can find some potential applications in the field of developing nanophotonic devices with excellent performance working at the mid-infrared regime.

13.
Mol Cell ; 76(6): 981-997.e7, 2019 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-31757757

RESUMO

Visualizing the location and dynamics of RNAs in live cells is key to understanding their function. Here, we identify two endonuclease-deficient, single-component programmable RNA-guided and RNA-targeting Cas13 RNases (dCas13s) that allow robust real-time imaging and tracking of RNAs in live cells, even when using single 20- to 27-nt-long guide RNAs. Compared to the aptamer-based MS2-MCP strategy, an optimized dCas13 system is user friendly, does not require genetic manipulation, and achieves comparable RNA-labeling efficiency. We demonstrate that the dCas13 system is capable of labeling NEAT1, SatIII, MUC4, and GCN4 RNAs and allows the study of paraspeckle-associated NEAT1 dynamics. Applying orthogonal dCas13 proteins or combining dCas13 and MS2-MCP allows dual-color imaging of RNAs in single cells. Further combination of dCas13 and dCas9 systems allows simultaneous visualization of genomic DNA and RNA transcripts in living cells.


Assuntos
Imagem Molecular/métodos , RNA/fisiologia , Imagem Individual de Molécula/métodos , Sistemas CRISPR-Cas/genética , Linhagem Celular Tumoral , Corantes Fluorescentes/química , Humanos , Mucina-4 , Engenharia de Proteínas/métodos , RNA Guia/genética , RNA Longo não Codificante , Ribonucleases/genética , Ribonucleases/metabolismo , Coloração e Rotulagem/métodos
14.
PeerJ ; 7: e7740, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31592192

RESUMO

Inverted cuttings of Populus yunnanensis exhibit an interesting growth response to inversion. This response is characterized by enlargement of the stem above the shoot site, while the upright stem shows obvious outward growth below the shoot site. In this study, we examined transcriptome changes in bark tissue at four positions on upright and inverted cuttings of P. yunnanensis: position B, the upper portion of the stem; position C, the lower portion of the stem; position D, the bottom of new growth; and position E, the top of new growth. The results revealed major transcriptomic changes in the stem, especially at position B, but little alteration was observed in the bark tissue of the new shoot. The differentially expressed genes (DEGs) were mainly assigned to four pathways: plant hormone signal transduction, plant-pathogen interaction, mitogen-activated protein kinase (MAPK) signaling pathway-plant, and adenosine triphosphate-binding cassette (ABC) transporters. Most of these DEGs were involved in at least two pathways. The levels of many hormones, such as auxin (IAA), cytokinin (CTK), gibberellins (GAs), ethylene (ET), and brassinosteroids (BRs), underwent large changes in the inverted cuttings. A coexpression network showed that the top 20 hub unigenes at position B in the upright and inverted cutting groups were associated mainly with the BR and ET signaling pathways, respectively. Furthermore, brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) in the BR pathway and both ethylene response (ETR) and constitutive triple response 1 (CTR1) in the ET pathway were important hubs that interfaced with multiple pathways.

15.
Biol Trace Elem Res ; 189(2): 511-518, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30215190

RESUMO

This study aimed to determine the effect of excessive fluoride (F) on the morphological characteristics of the small intestine and the contents of serum cytokines in rats. A total of 48 3-week-old healthy female Sprague-Dawley rats were randomly divided into four groups (n = 12). The control group was given deionized distilled water, while the F treatment groups were treated with water containing 25, 50, and 100 mg F-/L. After 70 days of treatment, the duodenum, the jejunum, and the ileum were collected to measure the developmental parameters and the distribution of intestinal glycoproteins, goblet cells, and mast cells through Pannoramic Viewer, Periodic Acid-Schiff (PAS) staining, Alcian blue and periodic acid-Schiff (AB-PAS) staining, and toluidine blue staining, respectively. The contents of cytokines, namely, interleukin (IL)-1ß, IL-2, IL-6, and tumor necrosis factor (TNF)-α, in serum were detected via enzyme-linked immunosorbent assay (ELISA). Results showed that the villus height, crypt depth, villus height to crypt depth ratio, goblet cells, glycoproteins, and mast cells of the small intestine significantly decreased (P < 0.05 or P < 0.01) in the F treatment group. The contents of IL-1ß, IL-2, IL-6, and TNF-α were significantly lower in the F treatment group than in the control group (P < 0.05 or P < 0.01). In summary, excessive F intake impaired intestinal development and immune function by decreasing the developmental parameters and the distribution of immune cells, glycoproteins, and cytokines.


Assuntos
Citocinas/sangue , Fluoretos/toxicidade , Intestino Delgado/efeitos dos fármacos , Intestino Delgado/metabolismo , Animais , Duodeno/efeitos dos fármacos , Duodeno/metabolismo , Feminino , Íleo/efeitos dos fármacos , Íleo/metabolismo , Interleucina-10/sangue , Interleucina-2/sangue , Jejuno/efeitos dos fármacos , Jejuno/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa/sangue
16.
Diab Vasc Dis Res ; 15(4): 322-335, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29762054

RESUMO

Sarco(endo)plasmic reticulum calcium adenosine triphosphatase is responsible for transporting cytosolic calcium into the sarcoplasmic reticulum and endoplasmic reticulum to maintain calcium homeostasis. Sarco(endo)plasmic reticulum calcium adenosine triphosphatase is the dominant isoform expressed in cardiac tissue, which is regulated by endogenous protein inhibitors, post-translational modifications, hormones as well as microRNAs. Dysfunction of sarco(endo)plasmic reticulum calcium adenosine triphosphatase is associated with heart failure, which makes sarco(endo)plasmic reticulum calcium adenosine triphosphatase a promising target for heart failure therapy. This review summarizes current approaches to ameliorate sarco(endo)plasmic reticulum calcium adenosine triphosphatase function and focuses on phospholamban, an endogenous inhibitor of sarco(endo)plasmic reticulum calcium adenosine triphosphatase, pharmacological tools and gene therapies.


Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Inibidores Enzimáticos/uso terapêutico , Terapia Genética/métodos , Insuficiência Cardíaca/terapia , Miócitos Cardíacos/efeitos dos fármacos , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/antagonistas & inibidores , Animais , Sinalização do Cálcio/efeitos dos fármacos , Proteínas de Ligação ao Cálcio/genética , Inibidores Enzimáticos/efeitos adversos , Regulação Neoplásica da Expressão Gênica , Insuficiência Cardíaca/enzimologia , Insuficiência Cardíaca/genética , Insuficiência Cardíaca/fisiopatologia , Humanos , Isoenzimas , Terapia de Alvo Molecular , Contração Miocárdica/efeitos dos fármacos , Miócitos Cardíacos/enzimologia , Miócitos Cardíacos/patologia , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/genética , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo
17.
Sci Total Environ ; 609: 1126-1131, 2017 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-28787787

RESUMO

Eight low-ring PAHs were detected in 21 polystyrene (PS) food contact materials (FCMs) samples while high-ring PAHs (>4 rings) were not found. This is because the reaction pathway for formation of high-ring PAHs consists of more steps than it does for low-high PAHs. The concentrations of Σ8PAH were from 18.9±5.16ng/g for product colorless fruit fork to 476±52.0ng/g for foam instant noodle container. These data were far beyond levels of PAHs in other plastics. Of the eight PAHs detected, Phe had the highest average concentration, followed by Nap. These two PAHs collectively accounted for over 80% of the Σ8PAH concentrations in all PS FCMs. Levels of Σ8PAH in expanded PS FCMs were higher than those in extruded ones due to utilization of foaming agent. The concentrations of Σ8PAH were lower in colorless PS FCMs than in colored ones. Auxochromes and chromophores contributed to the change of short-chain hydrocarbons to aromatic hydrocarbon. Simulated migration values of PAHs from PS FCMs to food varied widely. The migration value of Σ8PAH with maximum probability was below 10ng/g, which the maximum tolerated migration level for substance according to the European Union standards. However, higher migration values were possible and the potential health risk should still be concerned because the simulated migration displayed a log-normal distribution. Furthermore, water was used as food simulant would always lead to an underestimate of PAHs migration to real daily food, and then lead to an underestimate of risk.


Assuntos
Contaminação de Alimentos/análise , Embalagem de Alimentos , Hidrocarbonetos Policíclicos Aromáticos/análise , Poliestirenos , Monitoramento Ambiental , Hidrocarbonetos , Plásticos , Água
18.
Asian Pac J Cancer Prev ; 18(2): 571-576, 2017 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-28345847

RESUMO

Background: Five-year survival is an important metric for progress in cancer control broadly used both in the cancer literature and by the public. In order to assess its validity and relation to other common metrics, we analyzed the relationship between 5-year survival, incidence and mortality using publicly available cancer registry data from England and Wales. Methods: Five-year survival, incidence and mortality data were obtained from the online database of a registered charity, Cancer Research UK. We extracted sex-specific age-standardized mortality, incidence, and 5-year survivalfor16 types of cancer over the period from 1976 to 1995. The relationships between 5-year survival, incidence and mortality were estimated using both Pearson and Spearman correlation coefficients. Results: All 16cancer types showed an increase in 5-year survival for both genders from 1976 to 1995, ranging from 0.2% (pancreas and lung cancer) to 16.6% (prostate cancer) for males and 0.2% (pancreas cancer) to 16.6% (leukemia) for females. From 1976 to 1995, there was no significant correlation between changes in 5-year survival and cancer mortality for either sex (males, Pearson r=0.16, Spearman r=-0.06; females, Pearson r=-0.33, Spearman r=-0.43). A positive relationship between 5-year survival and incidence was noted among males, but not among females (males, Pearson r=0.61, Spearman r=0.53; females, Pearson r=0.03, Spearman r=0.11). However, after excluding breast and prostate cancer, the positive association became weaker and became statistically non-significant for males (Pearson r=0.47; Spearman r=0.41). Conclusions: Our findings suggest that there are no reliable relationships between changes in 5-year survival and cancer incidence or mortality. Increases in 5-year survival might therefore represent poor indicators of progress in cancer control at the population level. In the absence of over-diagnosis, 5-year survival might only indicate improved diagnosis and treatment in clinical practice.

19.
Huan Jing Ke Xue ; 38(10): 4034-4043, 2017 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965185

RESUMO

Size-resolved atmospheric particles were collected monthly from February 2012 to January 2013 on the campus of Chengdu University of Technology using the Anderson multi-stage impactor sampler. The mass concentrations of these samples and the contents of nine water-soluble ion species were analyzed. The results showed that the annual average concentrations of PM2.1 and PM11 in Chengdu during the sampling period were (125.9±56.14) µg·m-3 and (224.5±83.64) µg·m-3, respectively. The highest mass concentration of particulate matter was observed in winter, followed by spring while the lowest was in autumn. The average content of water-soluble ions in particulate matter in Chengdu was about 37.15 µg·m-3 and the order of water-soluble ion mass concentrations was SO42- > NO3- > NH4+ > Ca2+ > Cl- > Mg2+ > K+ > Na+ > F-. Meanwhile, SO42-, NO3-, and NH4+ were the major water-soluble ionic components that accounted for 78% of total water-soluble ions. Unimodal distribution was observed for SO42-, NO3-, and NH4+ mainly in fine particles, while Ca2+ and F- were concentrated in coarse particles and their distribution was also unimodal. Similar size distributions were found for Cl- and K+, as well as Mg2+ and Na+, both of which were bimodal. The content of water-soluble ions in both fine and coarse particles during winter and spring was significantly higher than that in summer and autumn in Chengdu. Based on the correlation analysis for major ions, SO42-, NO3-, and NH4+ in fine particles are likely in the form of (NH4)2SO4, NH4HSO4, and NH4NO3. In coarse particles, the major water-soluble ions (i.e. SO42-, NO3-, and Ca2+) might exist in the forms of Ca(NO3)2 and CaSO4. The secondary formation processes, soil dust, and biomass burning are the major sources of water-soluble ion species in the particulate matter in Chengdu.

20.
J Gen Virol ; 98(3): 486-495, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28008822

RESUMO

Duck enteritis virus (DEV) is an acute, septic, sexually transmitted disease that occurs in ducks, geese and other poultry. Autophagy is an evolutionarily ancient pathway that is important in many viral infections. Despite extensive study, the interplay between DEV and autophagy of host cells is not clearly understood. In this study, we found that DEV infection triggers autophagy in duck embryo fibroblast (DEF) cells, as demonstrated by the appearance of autophagosome-like double- or single-membrane vesicles in the cytoplasm of host cells and the number of GFP-LC3 dots. In addition, increased conversion of the autophagy marker protein LC3-I and LC3-II and decreased p62/SQSTM1 indicated complete autophagy flux. Heat-inactivated DEV infection did not induce autophagy, suggesting that the trigger of autophagy in DEF cells depended on DEV replication. When autophagy was pharmacologically inhibited by LY294002 or wortmannin, DEV replication decreased. The DEV offspring yield decreased when small interference RNA was used to interfere with autophagy related to the genes Beclin-1 and ATG5. In contrast, after treating DEF cells with rapamycin, an inducer of autophagy, DEV replication increased. These results indicated that DEV infection induced autophagy in DEF cells and autophagy facilitated DEV replication.


Assuntos
Autofagia , Mardivirus/fisiologia , Doença de Marek/virologia , Replicação Viral , Androstadienos/farmacologia , Animais , Autofagia/efeitos dos fármacos , Autofagia/genética , Proteína 5 Relacionada à Autofagia/genética , Proteína Beclina-1/genética , Cromonas/farmacologia , Patos , Fibroblastos/virologia , Proteínas Associadas aos Microtúbulos/metabolismo , Morfolinas/farmacologia , Fagossomos/metabolismo , Fagossomos/virologia , RNA Interferente Pequeno , Sirolimo/farmacologia , Wortmanina
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