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1.
Ther Apher Dial ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31574579

RESUMO

High body mass index (BMI) is the most common parameter to assess excess adiposity, and has been linked to glomerular hyperfiltration (GH). However, BMI may be misleading in the estimation of body fat content due to its inability to discriminate between body fat and lean mass. In recent years, the convenient biological impedance analysis (BIA) has made prediction of certain diseases somewhat feasible and accessible using body composition (BC). Accordingly, we conducted a cross-sectional study to explore the association between BC and GH among Chinese adult population. A total of 6902 adults (aged 38.6 ± 8.3 years, 70.1% males) who consecutively visited the Health Checkup Clinic were enrolled. BC including fat mass and lean body mass (LBM) was evaluated by BIA. The upper quartile of eGFR which exceeded 117.3 mL/min/1.73 m2 was defined as GH, in comparison with the lower three quartiles (control group). As a categorical outcome, GH subjects had higher fat/LBM than the control group, which was 34.7 ± 10.9 (%) vs. 34.0 ± 10.5 (%), P = 0.01; however, the BMI in GH group was lower than in the control group, which was 24.5 ± 3.7 (%) vs. 24.9 ± 3.6 (%), P<0.001. Fat/height and Fat/BSA were not significantly different between the two groups. Moreover, after adjusted for potential confounders, fat/LBM significantly correlated with GH (OR = 2.09, 95% CI, 1.11 to 3.93). The study revealed that fat/LBM significantly correlated with GH among Chinese adult population, which highlights that adiposity might be an important and potentially modifiable determinant of GH. This article is protected by copyright. All rights reserved.

2.
BMC Genomics ; 20(1): 648, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31412769

RESUMO

BACKGROUND: The hundred seed weight (HSW) is one of the yield components of soybean [Glycine max (L.) Merrill] and is especially critical for various soybean food types. In this study, a representative sample consisting of 185 accessions was selected from Northeast China and analysed in three tested environments to determine the quantitative trait nucleotide (QTN) of HSW through a genome-wide association study (GWAS). RESULT: A total of 24,180 single nucleotide polymorphisms (SNPs) with minor allele frequencies greater than 0.2 and missing data less than 3% were utilized to estimate linkage disequilibrium (LD) levels in the tested association panel. Thirty-four association signals were identified as associated with HSW via GWAS. Among them, nineteen QTNs were novel, and another fifteen QTNs were overlapped or located near the genomic regions of known HSW QTL. A total of 237 genes, derived from 31 QTNs and located near peak SNPs from the three tested environments in 2015 and 2016, were considered candidate genes, were related to plant growth regulation, hormone metabolism, cell, RNA, protein metabolism, development, starch accumulation, secondary metabolism, signalling, and the TCA cycle, some of which have been found to participate in the regulation of HSW. A total of 106 SNPs from 16 candidate genes were significantly associated with HSW in soybean. CONCLUSIONS: The identified loci with beneficial alleles and candidate genes might be valuable for the molecular network and MAS of HSW.

3.
Neuroscience ; 415: 147-160, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31369718

RESUMO

Stroke is a major life-threatening and disabling disease with a restricted therapeutic approach. Bone marrow stromal cells (BMSCs) possess proliferative ability and a multi-directional differentiation potential, and secrete a range of trophic/growth factors that can protect neurons after cerebral ischemia/reperfusion. Transient receptor potential canonical (TRPC) is a family of non-selective channels permeable to Ca2+, with several functions including neuronal survival. Over-expression of TRPC6, a subtype of the TRPC family, was shown to protect neurons against cerebral ischemia/reperfusion injury. However, it remains unclear whether over-expression of TRPC6 in BMSCs can further reduce brain injury after ischemia/reperfusion. In the present study, we report that over-expression of TRPC6 via a CRISPR-based synergistic activation mediator in BMSCs provided a greater reduction of brain injury in a rat model of ischemia/reperfusion. Further, the improved neurofunctional outcomes were associated with increased TRPC6 and brain derived neurotrophic factor expression levels. Overall, these data suggest that TRPC6 over-expressing BMSCs may be a promising therapeutic agent for ischemic stroke.

4.
PLoS One ; 14(8): e0221019, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31398217

RESUMO

As a new strategy to power forest wireless sensors in remote areas, an environmental microenergy collection device has been improved, and field experiments were carried out under natural conditions for the first time. The thermoelectric power generation devices used a gravity-assisted heat pipe to transmit heat from shallow soil to ground level, and a thermoelectric generator (TEG) was employed to generate electric power from the temperature difference between soil and air. Over the 6-month experimental period at two natural sites, approximately 128.74 J of energy could be harvested in a single day, and 5 209.92 J of energy could be harvested in a generation cycle. The results showed the feasibility of using this green energy to power wireless sensors in remote forests or other environments, This work is relevant to the current acute energy shortages and environmental pollution problems.

5.
Bioresour Technol ; 293: 122041, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31465897

RESUMO

Co-digesting liquid swine manure and brewery wastewater for hydrogen and methane production was studied using an integrated, two-phase system with different organic loading rates (OLR) under mesophilic conditions. The highest volumetric hydrogen and methane production rates achieved were 294.06 ±â€¯3.06 mL H2 L-1 d-1 and 497.94 ±â€¯10.01 mL CH4 L-1 d-1, respectively, at the OLR of 8613.6 mg COD L-1 d-1, together with the highest hydrogen-COD and -TVS yields (34.14 ±â€¯0.36 mL g-1 COD and 45.46 ±â€¯0.71 mL g-1 TVS) and the maximum methane-TVS yield (261.42 ±â€¯8.41 mL g-1 TVS). The highest hydrogen and methane concentrations in the biogas (31.86 ±â€¯0.68% and 82.66 ±â€¯0.33%, respectively) were also obtained at this OLR. The maximum methane-COD yield (270.3 ±â€¯3.93 mL g-1 COD), however, was from the lowest OLR (1148.6 mg COD L-1 d-1). The two-phase system removed 75.54 ±â€¯0.19% of COD from the influent.


Assuntos
Esterco , Águas Residuárias , Anaerobiose , Animais , Biocombustíveis , Reatores Biológicos , Hidrogênio , Metano , Suínos
6.
Hum Brain Mapp ; 40(16): 4801-4812, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31365184

RESUMO

Recently, graph theoretical approaches applied to neuroimaging data have advanced understanding of the human brain connectome and its abnormalities in psychiatric disorders. However, little is known about the topological organization of brain white matter networks in posttraumatic stress disorder (PTSD). Seventy-six patients with PTSD and 76 age, gender, and years of education-matched trauma-exposed controls were studied after the 2008 Sichuan earthquake using diffusion tensor imaging and graph theoretical approaches. Topological properties of brain networks including global and nodal measurements and modularity were analyzed. At the global level, patients showed lower clustering coefficient (p = .016) and normalized characteristic path length (p = .035) compared with controls. At the nodal level, increased nodal centralities in left middle frontal gyrus, superior and inferior temporal gyrus and right inferior occipital gyrus were observed (p < .05, corrected for false-discovery rate). Modularity analysis revealed that PTSD patients had significantly increased inter-modular connections in the fronto-parietal module, fronto-striato-temporal module, and visual and default mode modules. These findings indicate a PTSD-related shift of white matter network topology toward randomization. This pattern was characterized by an increased global network integration, reflected by increased inter-modular connections with increased nodal centralities involving fronto-temporo-occipital regions. This study suggests that extremely stressful life experiences, when they lead to PTSD, are associated with large-scale brain white matter network topological reconfiguration at global, nodal, and modular levels.

7.
Sensors (Basel) ; 19(14)2019 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-31330918

RESUMO

Mobile laser scanning (MLS) is widely used in the mapping of forest environments. It has become important for extracting the parameters of forest trees using the generated environmental map. In this study, a three-dimensional point cloud map of a forest area was generated by using the Velodyne VLP-16 LiDAR system, so as to extract the diameter at breast height (DBH) of individual trees. The Velodyne VLP-16 LiDAR system and inertial measurement units (IMU) were used to construct a mobile measurement platform for generating 3D point cloud maps for forest areas. The 3D point cloud map in the forest area was processed offline, and the ground point cloud was removed by the random sample consensus (RANSAC) algorithm. The trees in the experimental area were segmented by the European clustering algorithm, and the DBH component of the tree point cloud was extracted and projected onto a 2D plane, fitting the DBH of the trees using the RANSAC algorithm in the plane. A three-dimensional point cloud map of 71 trees was generated in the experimental area, and estimated the DBH. The mean and variance of the absolute error were 0.43 cm and 0.50, respectively. The relative error of the whole was 2.27%, the corresponding variance was 15.09, and the root mean square error (RMSE) was 0.70 cm. The experimental results were good and met the requirements of forestry mapping, and the application value and significance were presented.

8.
J Neurochem ; 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31314916

RESUMO

Glial glutamate transporter 1 (GLT-1) plays a vital role in the induction of brain ischemic tolerance (BIT) by ischemic preconditioning (IPC). However, the mechanism still needs to be further explained. The aim of this study was to investigate whether peroxisome proliferator-activated receptor gamma (PPARγ) participates in regulating GLT-1 during the acquisition of BIT induced by IPC. Initially, cerebral IPC induced BIT and enhanced PPARγ and GLT-1 expression in the CA1 hippocampus in rats. The ratio of nuclear/cytoplasmic PPARγ was also increased. At the same time, the up-regulation of PPARγ expression in astrocytes in the CA1 hippocampus was revealed by double immunofluorescence for PPARγ and glial fibrillary acidic protein. Then, the mechanism by which PPARγ regulates GLT-1 was studied in rat cortical astrocyte-neuron cocultures. We found that IPC [45 min of oxygen glucose deprivation (OGD)] protected neuronal survival after lethal OGD (4 h of OGD), which usually leads to neuronal death. The activation of PPARγ occurred earlier than the up-regulation of GLT-1 in astrocytes after IPC, as determined by western blot and immunofluorescence. Moreover, the preadministration of the PPARγ antagonist T0070907 or PPARγ siRNA significantly attenuated GLT-1 up-regulation and the neuroprotective effects induced by IPC in vitro. Finally, the effect of the PPARγ antagonist on GLT-1 expression and BIT was verified in vivo. We observed that the preadministration of T0070907 by intracerebroventricular injection dose-dependently attenuated the up-regulation of GLT-1 and BIT induced by cerebral IPC in rats. In conclusion, PPARγ participates in regulating GLT-1 during the acquisition of BIT induced by IPC. OPEN SCIENCE BADGES: This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/. Open Science: This manuscript was awarded with the Open Materials Badge For more information see: https://cos.io/our-services/open-science-badges/.

9.
Opt Express ; 27(12): 16833-16846, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31252903

RESUMO

The optimized design of multilayer-coated blazed gratings (MLBG) for high-flux tender X-ray monochromators was systematically studied by numerical simulations. The resulting correlation between the multilayer d-spacing and grating blaze angle significantly deviated from the one predicted by conventional equations. Three high line density gratings with different blaze angles were fabricated and coated by the same Cr/C multilayer. The MLBG with an optimal blaze angle of 1.0° showed a record efficiency reaching 60% at 3.1 keV and 4.1 keV. The measured efficiencies of all three gratings were consistent with calculated results proving the validity of the numerical simulation and indicating a more rigorous way to design the optimal MLBG structure.

10.
BMC Genomics ; 20(1): 519, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31234790

RESUMO

BACKGROUND: Banana wilt disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), is one of the most devastating diseases in banana (Musa spp.). Foc is a soil borne pathogen that causes rot of the roots or wilt of leaves by colonizing the xylem vessels. The dual RNA sequencing is used to simultaneously assess the transcriptomes of pathogen and host. This method greatly helps to understand the responses of pathogen and host to each other and discover the potential pathogenic mechanism. RESULTS: Plantlets of two economically important banana cultivars, Foc TR4 less susceptible cultivar NK and susceptible cultivar BX, were used to research the Foc-banana interaction mechanism. Notably, the infected NK had more significantly up-regulated genes on the respiration machinery including TCA cycle, glyoxylate, glycerol, and glycolysis compared to BX at 27 h post inoculation (hpi). In addition, genes involved in plant-pathogen interaction, starch, sucrose, linolenic acid and sphingolipid metabolisms were uniquely more greatly induced in BX than those in NK during the whole infection. Genes related to the biosynthesis and metabolism of SA and JA were greatly induced in the infected NK; while auxin and abscisic acid metabolisms related genes were strongly stimulated in the infected BX at 27 hpi. Furthermore, most of fungal genes were more highly expressed in the roots of BX than in those of NK. The fungal genes related to pathogenicity, pectin and chitin metabolism, reactive oxygen scavenging played the important roles during the infection of Foc. CCP1 (cytochrome c peroxidase 1) was verified to involve in cellulose utilization, oxidative stress response and pathogenicity of fungus. CONCLUSION: The transcriptome indicated that NK had much faster defense response against Foc TR4 than BX and the expression levels of fungal genes were higher in BX than those in NK. The metabolisms of carbon, nitrogen, and signal transduction molecular were differentially involved in pathogen infection in BX and NK. Additionally, the putative virulence associated fungal genes involved in colonization, nutrition acquirement and transport provided more insights into the infection process of Foc TR4 in banana roots.

11.
Chin Med J (Engl) ; 132(12): 1435-1440, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31205101

RESUMO

BACKGROUND: Previous studies have shown that endogenous T cells play an important role in the prolonged survival time of high-grade glioma (HGG) patients. Our objectives were to investigate the features of T-cell receptor (TCR) repertoires in HGG patients and to elucidate any potential therapeutic value. METHODS: During November 2011 and December 2018, tumor tissues and blood samples of 35 patients with HGG who underwent surgery at Beijing Tiantan Hospital or Beijing Shijitan Hospital were selected after surgery. After isolating DNA from samples, multiple rounds of PCR were performed to establish a DNA immune repertoire (IR). Then, the sequences and frequencies of the complementarity-determining 3 (CDR3) region in TCR beta chain (TRB) were identified by high-throughput sequencing and IR analysis. A survival follow-up was conducted monthly thereafter until December 2018. Finally, the t test and Mann-Whitney test were used to compare statistical differences between two sets of data. RESULTS: The Shannon diversity index (SHDI) of TRB sequences of HGG patients was significantly lower than that of healthy individuals (7.34 vs. 8.45, P = 0.001). The SHDI of TRB sequences of glioblastoma (GBM) patients with more than 16 months survival time was much higher than that of GBM patients with shorter survival times in both tumor tissues (3.48 ±â€Š0.31 vs. 6.21 ±â€Š0.33, t = -5.49, P = 0.002) and blood cells (6.02 ±â€Š0.66 vs. 7.44 ±â€Š0.32, t = -2.20, P = 0.036). In addition, patients achieved a distinctly higher proportion compared to that of healthy individuals in the proportion of TRBV9 and TRBV5 functional regions (9.83% vs. 6.83%, P = 0.001). Surgical tissue from patients who survived more than 16 months yielded a much higher proportion of TRBV4 and TRBV9 regions (7.14% vs. 3.28%, t = 3.18, P = 0.019). In surgical tissues from two GBM patients who survived for longer than 46 months, we found a potentially therapeutic TCR sequence. CONCLUSIONS: HGG patients have less species diversity of TCR repertoires compared with that of healthy individuals. TRBV9 regions in TCRs may be protective factors for long-term survival of GBM patients.

12.
Curr Pharm Des ; 25(10): 1041-1051, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31187705

RESUMO

The blood-brain barrier (BBB) is a barrier of the central nervous system (CNS), which can restrict the free exchange of substances, such as toxins and drugs, between cerebral interstitial fluid and blood, keeping the relative physiological stabilization. The brain capillary endothelial cells, one of the structures of the BBB, have a variety of ATP-binding cassette transporters (ABC transporters), among which the most widely investigated is Pglycoprotein (P-gp) that can efflux numerous substances out of the brain. The expression and activity of P-gp are regulated by various signal pathways, including tumor necrosis factor-α (TNF-α)/protein kinase C-ß (PKC- ß)/sphingosine-1-phosphate receptor 1 (S1P), vascular endothelial growth factor (VEGF)/Src kinase, etc. However, it remains unclear how hypoxic signaling pathways regulate the expression and activity of P-gp in brain microvascular endothelial cells. According to previous research, hypoxia affects the expression and activity of the transporter. If the transporter is up-regulated, some drugs enter the brain's endothelial cells and are pumped back into the blood by transporters such as P-gp before they enter the brain tissue, consequently influencing the drug delivery in CNS; if the transporter is down-regulated, the centrally toxic drug would enter the brain tissue and cause serious adverse reactions. Therefore, studying the mechanism of hypoxia-regulating P-gp can provide an important reference for the treatment of CNS diseases with a hypoxia/reoxygenation (H/R) component. This article summarized the mechanism of regulation of P-gp in BBB in normoxia and explored that of hypoxia.

13.
Cancer Med ; 8(10): 4527-4535, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31240876

RESUMO

BACKGROUND: Glioma-related epilepsy (GRE) is defined as symptomatic epileptic seizures secondary to gliomas, it brings both heavy financial and psychosocial burdens to patients with diffuse glioma and significantly decreases their quality of life. To date, there have been no clinical guidelines that provide recommendations for the optimal diagnostic and therapeutic procedures for GRE patients. METHODS: In March 2017, the Joint Task Force for GRE of China Association Against Epilepsy and Society for Neuro-Oncology of China launched the guideline committee for the diagnosis and treatment of GRE. The guideline committee conducted a comprehensive review of relevant domestic and international literatures that were evaluated and graded based on the Oxford Centre for Evidence-Based Medicine Levels of Evidence, and then held three consensus meetings to discuss relevant recommendations. The recommendations were eventually given according to those relevant literatures, together with the experiences in the diagnosis and treatment of over 3000 GRE cases from 24 tertiary level hospitals that specialize in clinical research of epilepsy, glioma, and GRE in China. RESULTS: The manuscript presented the current standard recommendations for the diagnostic and therapeutic procedures of GRE. CONCLUSIONS: The current work will provide a framework and assurance for the diagnosis and treatment strategy of GRE to reduce complications and costs caused by unnecessary treatment. Additionally, it can serve as a reference for all professionals involved in the management of patients with GRE.

14.
Zhongguo Zhong Yao Za Zhi ; 44(7): 1314-1320, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-31090286

RESUMO

Salvia miltiorrhiza is one of the commonly used bulk medicinal materials, which has significant effect on cardiovascular disease, and are heavy demanded in Asia, Europe, North America, Russia and Africa. Consequently, increasing the yield and quality of S. miltiorrhiza has become a major concern worldwide. With the current wild resources of S. miltiorrhiza gradually decreasing, cultivated products occupy most of the markets. However, the cultivation area is widely distributed and the cultivation techniques is different, which lead to the quality and yield of S. miltiorrhiza in consistent. This paper combined visiting survey with document analysis to carry out the cultivation situation of S. miltiorrhiza in main cultivation areas of Shandong, Henan, Sichuan, Shanxi and Hebei provinces. There exist big differences of the ecological environment, mode of cultivation, fertilization, pest control, harvesting processing among the producing areas. We should carry on the ecological suitability zoning analysis and suitable cultivation of each area study to form a pattern of high quality and high yield for the sustainable development of S. miltiorrhiza cultivation.


Assuntos
Agricultura/métodos , Salvia miltiorrhiza/crescimento & desenvolvimento , Europa (Continente) , Plantas Medicinais/crescimento & desenvolvimento
15.
AJR Am J Roentgenol ; 213(3): 667-671, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31063420

RESUMO

OBJECTIVE. The purpose of this study was to investigate the prevalence of white matter hyperintensity (WMH) without specific causes in young clinical outpatients. MATERIALS AND METHODS. A total of 1249 young clinical outpatients who underwent an unenhanced head MRI examination between January 1, 2016, and December 31, 2016, were included in the study. The chi-square test was used to analyze differences in the prevalence and characteristics of WMH by sex, age, and history of cardiovascular disease (CVD). The prevalence of WMH among clinical patients with neurologic symptoms was also compared with that among participants without neurologic symptoms. Logistic regression was used to identify the patient characteristics that were the best predictors of WMH. RESULTS. The overall prevalence of WMH was 25.94% (324/1249). Most patients with WMH (85.49% [277/324]) had mild WMH, mainly in frontal and parietal subcortical white matter. There was no significant difference in the prevalence of WMH by sex (p > 0.05), but the prevalence of WMH was higher among older patients (p < 0.001) and patients with a history of CVD (p < 0.001). Compared with participants without neurologic symptoms, clinical patients with dizziness (p = 0.029) and light-headedness (p = 0.001) were more likely to have WMH, which was attributed to older age and CVD. Logistic regression analysis showed that age and CVD were the best predictors of WMH. CONCLUSION. WMH is frequently found in young clinical patients. Most WMH is the mild type and mainly located in frontal and parietal subcortical white matter. Older age and CVD are risk factors for WMH.

16.
J Immunother ; 42(6): 215-220, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31145232

RESUMO

The effect of chemotherapy on programmed cell death-ligand 1 (PD-L1) expression has been previously studied in lung cancer, while the results remain controversial. The aim of this study was to investigate the variation of PD-L1 expression after neoadjuvant chemotherapy and explore the association between chemotherapy response, prognosis and the variation of PD-L1 expression in lung cancer patients. A total of 63 lung cancer patients who received platinum-based neoadjuvant chemotherapy and subsequently underwent surgical resection were selected. PD-L1 expression on tumor cells (TC) and tumor-infiltrating immune cells (IC) was assessed by immunohistochemistry using 22C3 monoclonal antibody in these 63 matched lung cancer specimens before and after neoadjuvant chemotherapy. The positivity of PD-L1 on TC changed from 17.5% to 39.7% after neoadjuvant chemotherapy and the positivity of PD-L1 on IC changed from 19.0% to 71.4% after neoadjuvant chemotherapy. The elevation of PD-L1 expression on TC after neoadjuvant chemotherapy was more frequently observed in patients achieving stable disease or progressive disease than in patients achieving partial response (P=0.026). Patients with elevated PD-L1 expression on TC after neoadjuvant chemotherapy showed a trend to have a shorter progression-free survival than patients without elevated PD-L1 expression on TC, although the difference was not statistically significant in multivariate analysis (hazard ratio=2.38, 95% confidence interval=0.99-5.73, P=0.053). PD-L1 expression can be elevated by chemotherapy in lung cancer. Furthermore, elevation of PD-L1 expression on TC after neoadjuvant chemotherapy was associated with reduced chemotherapy response and inferior progression-free survival in patients with lung cancer.

17.
Neurosci Lett ; 705: 206-211, 2019 07 13.
Artigo em Inglês | MEDLINE | ID: mdl-31005651

RESUMO

Development of a reliable biomarker for prognostic monitoring of cognitive impairment after traumatic brain injury (TBI) is of great importance. The aim of the study was to explore the value of early diffusional kurtosis imaging (DKI) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in evaluation of chronic cognitive function after TBI. MRI was performed on TBI and control rats at 7 days post-injury. MRI parameters were measured in bilateral cortex, hippocampus, thalamus and corpus callosum (CC). All the rats underwent Morris water maze (MWM) at 6 months after injury. Immunohistochemistry (IHC) analysis of neuron [NeuN], astroglia [GFAP], microglia [Iba-1], and myelin [MBP] was performed after the MWM test. Our study revealed that, TBI group showed higher volume transfer coefficient (Ktrans) value in ipsilateral cortex (P < 0.0001) and no detectable changes in other regions-of-interest (ROIs), compared with control group. DKI showed higher MK in all ipsilateral ROIs (P < 0.05), higher MD in ipsilateral cortex, hippocampus and CC (P < 0.05 for all) in TBI group. TBI group had worse performance in MWM test at 6 months post-injury(P < 0.05). IHC analysis showed lower NeuN, and higher GFAP and Iba-1 in all ipsilateral ROIs (P < 0.05) in TBI rats. NeuN, and GFAP and Iba-1 correlated significantly with MK value in ipsilateral regions of cortex. The MK value of ipsilateral cortex and CC and Ktrans value of ipsilateral cortex also correlated significantly with time in the target quadrant. Therefore, our study indicated that early DKI and DCE-MRI could be used to assess the microstructural changes associated with long-term cognitive outcome following TBI.

18.
Sheng Li Xue Bao ; 71(2): 336-342, 2019 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-31008494

RESUMO

Drug metabolism is significantly affected under hypoxia environment with changes of pharmacokinetics, expression and function of drug-metabolizing enzymes and transporters. Studies have shown that hypoxia increases the release of a series of inflammatory cytokines which can modulate drug metabolism. Besides, both hypoxia inducible factor 1α (HIF-1α) and microRNA-mediated pathways play a role in regulating drug metabolism. This article reviewed the impact and single-factor modulating mechanisms of drug metabolism under hypoxia, and put forward the speculation and prospects of multi-factor modulating mechanisms.


Assuntos
Subunidade alfa do Fator 1 Induzível por Hipóxia/fisiologia , Proteínas de Membrana Transportadoras/fisiologia , MicroRNAs/fisiologia , Preparações Farmacêuticas/metabolismo , Hipóxia Celular , Humanos , Hipóxia
19.
Sci Rep ; 9(1): 5990, 2019 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-30979945

RESUMO

As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin proteins containing late embryogenesis abundant (LEA2) domain. GmSYP24 was identified as a dehydration-responsive gene. Our study showed that the GmSYP24 protein was located on the cell membrane. The overexpression of GmSYP24 (GmSYP24ox) in soybean and heteroexpression of GmSYP24 (GmSYP24hx) in Arabidopsis exhibited insensitivity to osmotic/drought and high salinity. However, wild type soybean, Arabidopsis, and the mutant of GmSYP24 homologous gene of Arabidopsis were sensitive to the stresses. Under the abiotic stresses, transgenic soybean plants had greater water content and higher activities of POD, SOD compared with non-transgenic controls. And the leaf stomatal density and opening were reduced in transgenic Arabidopsis. The sensitivity to ABA was decreased during seed germination of GmSYP24ox and GmSYP24hx. GmSYP24hx induced up-regulation of ABA-responsive genes. GmSYP24ox alters the expression of some aquaporins under osmotic/drought, salt, or ABA treatment. These results demonstrated that GmSYP24 played an important role in osmotic/drought or salt tolerance in ABA signal pathway.

20.
Sensors (Basel) ; 19(5)2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30866505

RESUMO

To efficiently harvest environmental micro-energy from shallow soil, simulated analysis, theoretical arithmetic and experimental verification are performed to explore the spatiotemporal rules of heat transfer on a soil/finned tube interface. Simulations are carried out for 36 types of different working conditions, and the empirical formulas for temperature and heat flux are obtained. The temperature and heat flux can be calculated using the formulas if the soil temperature, soil moisture content and finned tube initial temperature are known. The simulations also show that the highest heat flux can reach approximately 0.30 mW/mm², and approximately 1507.96 mW of energy can be harvested through the finned tube. Theoretical arithmetic indicates that the heat transfer rate of the copper finned tube is 76.77% higher than that of the bare tube, the highest rate obtained in any study to date. Results also show that the finned tube should be placed where the soil moisture is greater than 30% to get more heat from the soil. A field experiment is carried out in the city of Harbin in Northeast China, where a thermoelectric power generation device has been installed and temperature data have been monitored for a certain time. The results are in good agreement with those obtained from the simulation analysis. The heat transfer processes and heat transfer steady state on the soil/finned tube interface are revealed in this work and are of great importance for the use of geothermal energy.

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