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1.
Artigo em Inglês | MEDLINE | ID: mdl-32134376

RESUMO

A coccobacilli-shaped proteobacterium, designated strain SYSU XM001T, was isolated from an activated sludge sample collected from JiMei sewage treatment plant, Xiamen, PR China. Cells were Gram-stain-negative, catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain SYSU XM001T shared less than 92 % sequence identities with members of the class Alphaproteobacteria, with highest sequence similarity to Aquidulcibacter paucihalophilus TH1-2T (91.6 %; family Hyphomonadaceae, order Rhodobacterales). The strain exhibited growth at 25-37 °C, pH 7.0-9.0 and in the presence of up to 1 % (w/v) NaCl. Its chemotaxonomic features included ubiquinone-10 as the respiratory isoprenologue, iso-C16 : 0, 10-methyl C16 : 0 TSBA and anteiso-C17 : 0 as major cellular fatty acids and monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids as the main polar lipids. The DNA G+C content was determined to be 62.9 % (draft genome). Analyses of the phylogenetic data and differences in the chemotaxonomic and biochemical features from related genera in the family Hyphomonadaceae indicated that strain SYSU XM001T merits representation of a novel species of a novel genus, for which the name Vitreimonas flagellata gen. nov., sp. nov. is proposed. The type strain of Vitreimonas flagellata is SYSU XM001T (=CGMCC 1.16661T=KCTC 62915T).

2.
Comp Immunol Microbiol Infect Dis ; 70: 101452, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32120143

RESUMO

Canine babesiosis is an important global tick-borne infectious disease of domestic dogs and wild canids. B. gibsoni and B. vogeli are the most widespread species mainly endemic in dog population in southern and eastern regions of China. In this study, 272 blood samples were collected from pet dogs in five districts of Shenzhen, China. Babesia DNA was detected in 30 samples with an overall prevalence of 11.0 % in pet dogs in Shenzhen. The difference in the positive rate between female and male pet dogs, among different breed, and among the five sampling sites was not significant, while the age and health status of pet dogs significantly influence the positive rate of Babesia spp. infection. Sequencing results showed the presence of only B. vogeli in all of the samples tested. Sequence comparison and phylogenetic analysis revealed that all 30 strains of Babesia identified in this study shared the highest identity with B. vogeli. In sum, all results of this study showed a considerable high infection rate of Babesia spp. in pet dogs, and B. vogeli was the only Babesia species infecting pet dogs in Shenzhen city. Such information is necessary to help to elucidate the epidemiology of canine babesiosis in Shenzhen, and it is necessary to actively monitor this disease in pet dogs.

3.
Cancer Res ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32193289

RESUMO

Multiple myeloma (MM) is an incurable refractory hematological malignancy arising from plasma cells in the bone marrow. Here we investigated miR-26a function in MM and tested single-wall carbon nanotube delivery of miR-26a in vitro and in vivo. miR-26a was downregulated in patient MM cells compared with plasma cells from healthy donors. miR-26a overexpression inhibited proliferation and migration and induced apoptosis in MM cell lines. To identify the targets of miR-26a, RPMI8226-V-miR-26-GFP and RPMI8226-V-GFP cells were cultured using Stable isotope labeling by amino acids in cell culture (SILAC) medium followed by mass spectrometry analysis. In MM cells overexpressing miR-26a, CD38 protein was downregulated and subsequently confirmed to be a direct target of miR-26a. Depletion of CD38 in MM cells duplicated the MM inhibition observed with exogenous expression of miR-26a, whereas restoration of CD38 overcame the inhibition of miR-26a in MM cells. In a human MM xenograft mouse model, overexpression of miR-26a inhibited CD38 expression, provoked cell apoptosis, and inhibited cell proliferation. Daratumumab is the first CD38 antibody drug for monotherapy and combination therapy for MM patients, but eventually resistance develops. In MM cells, CD38 remained at low level during daratumumab treatment, but a high quality response is sustained. In daratumumab-resistant MM cells, CD38 expression was completely restored but failed to correlate with daratumumab-induced cell death. Therefore, a therapeutic strategy to confer selection pressure to maintain low CD38 expression in MM cells may have clinical benefit.

4.
Prep Biochem Biotechnol ; : 1-8, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32125248

RESUMO

Echinocandin B, a kind of antimycotic with cyclic lipo-hexapeptides, was produced by fermentation with Aspergillus nidulans using fructose as main carbon source. The objective of this study was to screen a high-yield mutant capable of using cheap starch as main carbon source by atmospheric and room temperature plasma (ARTP) treatment in order to decrease the production cost of echinocandin B. A stable mutant A. nidulans ZJB19033, which can use starch as optimal carbon source instead of expensive fructose, was selected from two thousands isolates after several cycles of ARTP mutagenesis. To further increase the production of echinocandin B, the optimization of fermentation medium was performed by response surface methodology (RSM), employing Plackett-Burman design (PBD) followed by Box-Behnken design (BBD). The optimized fermentation medium provided the optimal yield of echinocandin B, 2425.9 ± 43.8 mg/L, 1.3-fold compared to unoptimized medium. The results indicated that the mutant could achieve high echinocandin B production using cheap starch as main carbon source, and the cost of carbon sources in fermentation medium reduced dramatically by about 45%.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32039746

RESUMO

A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2T, was isolated from oil-contaminated water which was collected from Xinjiang Province, north-west PR China. The 16S rRNA gene sequence analysis showed that strain 4-2T belonged to the genus Paracoccus. The species with highest similarity to strain 4-2T was Paracoccus saliphilus YIM 90738T (97.83 %), followed by 'Paracoccus siganidrum' M26 (97.83 %) and Paracoccus endophyticus SYSUP0003T (97.25 %). The average nucleotide identity values between 4-2T and three type strains were 84.69, 77.88 and 74.07 %, respectively. The genomic DNA G+C content of strain 4-2T was 61.4 mol%. Chemotaxonomical characteristic results showed that the respiratory quinone was ubiquinone Q-10 and the major fatty acids were summed feature 8 (C18 : 1 ω7c or C18 : 1 ω6c) and C19 : 0 cyclo ω8c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid and an unidentified polar lipid. The predominant polyamines were putrescine, cadaverine and spermidine. On the basis of phenotypic, chemotaxonomic and phylogenetic inferences, strain 4-2T represents a novel species of the genus Paracoccus, for which the name Paracoccus alkanivorans sp. nov. is proposed. The type strain is 4-2T (=CGMCC 1.13669T=LMG 30882T).

6.
Artigo em Inglês | MEDLINE | ID: mdl-32043955

RESUMO

A novel thermotolerant bacterial strain was isolated from a hot spring in a Tibetan geothermal field. Phylogenetic analysis of the 16S rRNA gene sequence of the novel strain showed that it belongs to a distinct lineage far from any known taxa. The new isolate shared the highest pairwise sequence identity with Phreatobacter cathodiphilus S-12T (92.8 % similarity) according to the 16S rRNA gene sequences. Cells were Gram-stain-negative, aerobic, rod-shaped and formed white round colonies. The strain grew at the ranges of 28-45 °C (optimum, 37 °C), pH 5.0-7.0 (optimum, pH 6.0) and 0-2 % NaCl. The strain was positive for catalase and oxidase. The major respiratory quinone was ubiquinone Q-10. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The DNA G+C content was 68.3 mol%. Based on these distinguishing properties, this strain is proposed to represent a new species of a new genus Rhabdaerophilum calidifontis gen. nov., sp. nov., within a new family Rhabdaerophilaceae fam. nov. The type strain of the type species of Rhabdaerophilum calidifontis is SYSU G02060T (=KCTC 72351T=CGMCC 1.17070T).

7.
Glob Chang Biol ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32037661

RESUMO

Active fires are considered to be the key contributor to, and critical consequence of, climate change. Quantifying the occurrence frequency and regional variations in global active fires is significant for assessing carbon cycling, atmospheric chemistry, and postfire ecological effects. Multiscale variations in fire occurrence frequencies have still never been fully investigated despite free access to global active fire products. We analyzed the occurrence frequencies of Visible Infrared Imaging Radiometer Suite (VIIRS) active fires at national, pan-regional (tropics and extratropics) to global scales and at hourly, monthly, and annual scales during 2012-2017. The results revealed that the accumulated occurrence frequencies of VIIRS global active fires were up to 12,193 × 104 , yet exhibiting slight fluctuations annually and with respect to the 2014-2016 El Niño event, especially during 2015. About 35.52% of VIIRS active fires occurred from July to September, particularly in August (13.06%), and typically between 10:00 and 13:00 Greenwich Mean Time (GMT; 42.96%) and especially at 11:00 GMT (17.65%). The total counts conform to a bimodal pattern with peaks in 5°-11°N (18.01%) and 5°-18°S (32.46%), respectively, alongside a unimodal distribution in terms of longitudes between 15°E and 30°E (32.34%). Tropical annual average of active fire (1,496.81 × 104 ) accounted for 75.83%. Nearly 30% were counted in Brazil, the Democratic Republic of the Congo, Indonesia, and Mainland Southeast Asia (MSEA). Fires typically occurred between June (or August) and October (or November) with far below-average rainfall in these countries, while those in MSEA primarily occurred between February and April during the dry season. They were primarily observed between 00:00 and 02:00 GMT, between 12:00 and 14:00 within each Zone Time. We believed that VIIRS global active fires products are useful for developing fire detection algorithms, discriminating occurrence types and ignition causes via correlation analyses with physical geographic elements, and assessment of their potential impacts.

8.
Nature ; 578(7795): 425-431, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32051592

RESUMO

Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of 735 kb, which is-to our knowledge-the largest phage genome to be described to date. Thirty-five genomes were manually curated to completion (circular and no gaps). Expanded genetic repertoires include diverse and previously undescribed CRISPR-Cas systems, transfer RNAs (tRNAs), tRNA synthetases, tRNA-modification enzymes, translation-initiation and elongation factors, and ribosomal proteins. The CRISPR-Cas systems of phages have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. In addition, some phages may repurpose bacterial CRISPR-Cas systems to eliminate competing phages. We phylogenetically define the major clades of huge phages from human and other animal microbiomes, as well as from oceans, lakes, sediments, soils and the built environment. We conclude that the large gene inventories of huge phages reflect a conserved biological strategy, and that the phages are distributed across a broad bacterial host range and across Earth's ecosystems.

9.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 24-28, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027248

RESUMO

OBJECTIVE: To investigate the clinical manifestations and laboratory features of B-ALL patients with EP300-ZNF384 fusion gene positive, so as to improve the understanding of this subtype disease. METHODS: The clinical data of 3 B-ALL patients with EP300-ZNF384 fusion gene positive admitted in Department of Hematology, the first medical center of Chinese PLA general hospital from February 2017 to February 2018 were collected and analyzed retrospectively. The clinical and laboratory characteristics as well as the therapentic outcome in B-ALL patients with EP300-ZNF384 fusion gene positive were analyzed. RESULTS: The fusion gene of EP300-ZNF384 was detected in 8.1%(3/37) of B-ALL patients. All cases showed the normal karyotype and aberrant CD13 and/or CD33 expression for immunophenotype. 3 patients were sensitive to traditional chemotherapy. CONCLUSION: The B-ALL with EEP300-ZNF384 fusion gene positive may be a subgroup of B-ALL with a uniqe clinical characteristis and laboatorial features. EP300-ZNF384 positive patients show a good response to conventional chemotherapy, suggesting a favorable prognosis.


Assuntos
Leucemia-Linfoma Linfoblástico de Células Precursoras , Proteína p300 Associada a E1A , Humanos , Proteínas de Fusão Oncogênica , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Estudos Retrospectivos , Transativadores , Fatores de Transcrição
10.
Clin Infect Dis ; 2020 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-31955202

RESUMO

BACKGROUND: Diabetes was identified as a tuberculosis (TB) risk factor mostly in retrospective studies with limited assessment of metabolic variables. The prospective Effects of Diabetes on Tuberculosis Severity study compared adults with pulmonary TB in Chennai, India, who were classified as having diabetes or normal glucose tolerance at enrollment. METHODS: Baseline TB severity, sputum conversion, and treatment outcomes (cure, failure, death, or lost) were compared between groups with respect to glycemic status and body mass index (BMI). RESULTS: The cohort of 389 participants included 256 with diabetes and 133 with normal glucose tolerance. Low BMI (<18.5 kg/m2) was present in 99 (74.4%) of non-diabetic participants and 85 (33.2%) of those with diabetes. Among participants with normal or high BMI, rates of cure, treatment failure, or death did not vary by glycemic status. Participants with low BMI had the highest radiographic severity of disease, the longest time to sputum culture conversion, and the highest rates of treatment failure and death. Among participants with low BMI, poorly controlled diabetes (HbA1c ≥8.0%) was unexpectedly associated with better TB treatment outcomes. High visceral adiposity index was associated with adverse outcomes and despite overall correlation with HbA1c, was elevated in some low BMI individuals with normal glucose tolerance. CONCLUSIONS: In this South Indian cohort, low BMI was significantly associated with increased risk for adverse TB treatment outcomes while comorbid poorly controlled diabetes lessened that risk. High visceral adiposity index with or without dysglycemia might reflect a novel TB susceptibility mechanism linked to adipose tissue dysfunction.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31980979

RESUMO

A novel proteobacterial strain designated SYSU H10001T was isolated from a soil sample collected from plateau meadow in Hongyuan county, Sichuan province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU H10001T was most closely related to Lysobacter soli KCTC 22011T (98.6%, sequence similarity) and Lysobacter panacisoli JCM 19212T (98.2%). The prediction result of secondary metabolites based on genome shown that the strain SYSU H10001T contained 3 clusters of bacteriocins, 1 cluster of non-ribosomal peptide synthetase, 1 cluster of type 1 polyketide synthase and 1 cluster of arylpolyene. In addition, the major isoprenoid quinone was Q-8 and the major fatty acids were identified as iso-C15:0, iso-C17:0 and Summed feature 9. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and three unidentified phospholipids. The genomic DNA G + C content of strain SYSU H10001T was 66.5% (genome). On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU H10001T represents a novel species of the genus Lysobacter, for which the name Lysobacter prati sp. nov. is proposed. The type strain is SYSU H10001T (= KCTC 72062T = CGMCC 1.16662T).

12.
Artigo em Inglês | MEDLINE | ID: mdl-31985395

RESUMO

China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated. SYSU G01001T and SY-13 were Gram-stain-positive, motile and spore-forming. Colonies were white, circular, raised and punctiform. SYSU G01001T and SY-13 grew at pH 6.0-9.0 (optimum pH 8.0) and at 23-37 °C (optimum 28 °C). The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species. On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species. The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L). The G+C content of the genomic DNA was 53.9 mol%. The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp. nov. is proposed. The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).

13.
Artigo em Inglês | MEDLINE | ID: mdl-31995462

RESUMO

A Gram-staining-positive, aerobic, non-motile, non-spore-forming and coccoid-shaped actinobacterial strain, designated YIM 730233T, was isolated from a sediment sample, collected from a hot spring in Tibet, China. Colonies were brownish, circular, smooth and convex. Strain YIM 730233T was able to grow in the temperature range of 20-50 °C, pH 6.5-8.0 and in the presence of up to 1.0 % (w/v) NaCl. A comparison of the 16S rRNA gene sequence of strain YIM 730233T with sequences of type strains of most closely related species of Marmoricola showed highest sequence similarities to Marmoricola bigeumensis MSL-05T (98.3%) and Marmoricola pocheonensis Gsoil 818T (98.1%). The draft genome of strain YIM 730233T had a size of 4 806 234 bp with a DNA G+C content of 72.1 mol%. The major fatty acids (>10 %) of strain YIM 730233T mainly consisted of iso-C16 : 0, anteiso-C17 : 0 and C18 : 1 ω9c, typical of the genus Marmoricola. Strain YIM 730233T had LL-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall. The predominant isoprenoid quinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and an unidentified lipid. DNA-DNA hybridizations between strain YIM 730233T and type strains of Marmoricola bigeumensis MSL-05T and Marmoricola pocheonensis Gsoil 818T resulted in similarity values of 21 and 19% respectively. Based on DNA-DNA hybridization results, together with the differentiating biochemical and chemotaxonomic features, showed that strain YIM 730233T represents a novel Marmoricola species, for which the name Marmoricola caldifontis sp. nov. (type strain YIM 730233T=KCTC 49192T=CGMCC 4.7521T), is proposed.

14.
Thorac Cancer ; 11(3): 561-569, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31971663

RESUMO

BACKGROUND: To investigate whether human papillomavirus (HPV) infection is associated with primary lung cancer among the Fujian population. METHODS: HPV infection was detected in 140 pairs of lung cancer tissues and matched paracancerous tissues by examining the 21 clinically relevant HPV types using a combination of viral highly conserved L1 region PCR amplification and specific probe reverse hybridization. Paired χ2 test was used to analyze differences in detection rates of HPV between lung cancer and paracancerous tissues. Differences in detection rates of HPV in lung cancer tissues were analyzed using χ2 test or the exact probability method. The rank sum test was used to analyze differences in the distributions of routine indices of blood and pulmonary function in lung cancer tissues between the HPV negative and positive groups. RESULTS: HPV infection was detected in 13 of the 140 tumor specimens and in 16 of the paired normal lung tissues. There was no significant correlation between HPV infection and lung cancer (P > 0.05). The diagnosed HPV infection rates did not differ significantly among lung cancer tissues with different stratification (P > 0.05). However, the platelet count, platelet pressure, residual gas volume, functional residual volume, and residual gas volume/lung total distribution may differ between HPV-negative and HPV-positive lung cancer tissues (0.000625 < P < 0.05). CONCLUSIONS: We concluded that HPV infection may not be associated with the risk of primary lung cancer in the Fujian population. However, HPV infection may affect platelet and residual lung function in primary lung cancer patients.

15.
JCI Insight ; 5(3)2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-31945014

RESUMO

Current models of B lymphocyte biology posit that B cells continuously recirculate between lymphoid organs, without accumulating in peripheral healthy tissues. Nevertheless, B lymphocytes are one of the most prevalent leukocyte populations in the naive murine heart. To investigate this apparent inconsistency in the literature, we conducted a systematic analysis of myocardial B cell ontogeny, trafficking dynamics, histology, and gene expression patterns. We found that myocardial B cells represent a subpopulation of circulating B cells that make close contact with the microvascular endothelium of the heart and arrest their transit as they pass through the heart. The vast majority (>95%) of myocardial B cells remain intravascular, whereas few (<5%) myocardial B cells cross the endothelium into myocardial tissue. Analyses of mice with B cell deficiency or depletion indicated that B cells modulate the myocardial leukocyte pool composition. Analysis of B cell-deficient animals suggested that B cells modulate myocardial growth and contractility. These results transform our current understanding of B cell recirculation in the naive state and reveal a previously unknown relationship between B cells and myocardial physiology. Further work will be needed to assess the relevance of these findings to other organs.

16.
Anal Biochem ; 592: 113566, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31904351

RESUMO

A sandwich-type immunosensor for detecting the concentration of carcinoembryonic antigen (CEA) was prepared. In this work, gold nanoparticles (Au NPs) were used as platform to attach more primary antibody (Ab1) due to excellent electrical conductivity and good biocompatibility. Molybdenum disulfide-Cerium oxide (CeO2-MoS2) nanohybrid was used as a carrier to absorb lead ions (Pb2+) and the second antibody (Ab2). CeO2-MoS2-Pb2+-Ab2 was used as a nanoprobe to detect CEA antigen. Under optimal conditions, square wave voltammetry (SWV) successfully displayed the electrical signal of Pb2+. The designed electrochemical immunosensor has excellent analytical performance. In addition, the detection range was 0.001-80 ng/mL and the minimum detection limit was 0.3 pg/mL (S/N = 3), which had good selectivity and stability. Finally, the proposed immunosensor successfully detected the concentration of CEA in the serum of the sample, which provided a feasible method for CEA testing.

17.
Sci Total Environ ; 713: 136556, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-31962243

RESUMO

The phenomenon of urban heat islands has been extensively investigated in recent decades. Due to the complexity of urban systems, this phenomenon may be scale-dependent, particularly for large megaregions where a cluster of cities gather together. Despite many studies focusing on urban heat islands at scales from single-site to regional, and further to global, there are few studies addressing multi-scale effects of large urban agglomeration on thermal environment. In this study, we used the Yangtze River Delta (YRD), one of China's most important megaregions, as a pilot case to examine the spatial-temporal variations of thermal environment and its driving forces at two spatial scales in 2000, 2005 and 2010. At regional scale, the effect of the entire megaregion on thermal environment was characterized by the distribution of the highest surface temperature zone (HTZ), which was closely related to the occurrence of continuously developed land. At city scale, the effect of individual city on thermal environment was characterized by the mean land surface temperature difference (LSTD) between urban and rural areas, which showed a significant positive correlation with the economic factors. In the YRD, the secondary industry output could explain approximately 58% and 39% of the variation of the LSTD in 2000 and 2005, respectively, while in 2010 the tertiary industry output became the important factor and accounted for 36% of the variation of LSTD. Finally, cities with fast urban economic growth rate and large size of urban areas were the priority for adopting more efficient strategies to urban thermal management.

18.
Nanotechnology ; 31(18): 185605, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31995543

RESUMO

Sandwich-type electrochemical immunosensor was one of the main methods for detecting carcinoembryonic antigen (CEA). In this work, using Ce-MoF as the skeleton precursor, hyaluronic acid (HA) was coated on the surface of Ce-metal organic framework (Ce-MoF), which loaded with silver nanoparticles (Ag NPs) and horseradish peroxidase (HRP) to catalyze H2O2 and double amplified the current signal. Thus, a sensitive sandwich-type electrochemical immunosensor (Ce-MoF@ HA/Ag-HRP) was designed to detect carcinoembryonic antigen (CEA). The designed immunosensor used Au NPs to enhance the ability of attach more the first antibody (Ab1). This was due to Au NPs had good electrical conductivity and biocompatibility to accelerate electron transfer on the surface of the electrode. HA was riched in -COOH, -OH and had excellent biocompatibility, which can carry more Ag NPs to catalyze H2O2. Finally, the prepared sandwich-type electrochemical immunosensor had excellent biocompatibility and great catalytic performance. The immunosensor can be tested within 30 min and the logarithm of the current signal and CEA concentration showed a broad linear response range of 1 pg ml-1-80 ng ml-1, and the detection limit of CEA was 0.2 pg ml-1. More importantly, the proposed immunosensor had good reproducibility, selectivity, stability and without matrix effect. This confirmed that the proposed immunosensor had broad prospects in early clinical trials.

19.
Nat Commun ; 11(1): 58, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31896757

RESUMO

Here we demonstrate a magnetic resonance coupling based wireless triboelectric nanogenerator (TENG) and fully self-powered wireless sensors. By integrating a microswitch and an inductor with the TENG, the pulsed voltage output is converted into a sinusoidal voltage signal with a fixed frequency. This can be transmitted wirelessly from the transmit coil to the resonant-coupled receiver coil with an efficiency of 73% for a 5 cm distance between the two coils (10 cm diameter). Analytic models of the oscillating and coupled voltage signals for the wireless energy transfer are developed, showing excellent agreement with the experimental results. A TENG of 40 × 50 mm2 can wirelessly light up 70 LEDs or charge up a 15 µF capacitor to 12.5 V in ~90 s. The system is further utilized for two types of fully self-powered wireless chipless sensors with no microelectronic components. The technologies demonstrate an innovative strategy for a wireless 'green' power source and sensing.

20.
Pharmacology ; : 1-11, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910411

RESUMO

BACKGROUND: Gastric cancer has become the second major cause of cancer death. The aim of the present study was to explore the relationship between miR-21 and the long noncoding RNA growth arrest-specific 5 (GAS5) in gastric cancer and the effect on gastric cancer cells. METHODS: The expression of miR-21 and GAS5 mRNA was analyzed by quantitative real-time-PCR. Overexpression of GAS5 was used to investigate the biological functions of GAS5 in cells. The cell proliferation was detected by cell counting kit-8 assay and the cell migration and invasion were detected by Transwell. Cell apoptosis was evaluated by Annexin V-FITC/PI staining and apoptosis-related proteins were detected by western blot. The mechanism of GAS5 in vivo was evaluated by the tumorigenesis of nude mice, and dual luciferase reporter was used to determine if miR-21 is a GAS5 target. The inhibition of miR-21 and the simultaneous overexpression of GAS5 and miR-21 were further performed, and the above indicators were detected again. RESULTS: GAS5 was low expression and miR-21 was high expression in gastric cancer tissues and cells. GAS5 overexpression reduced the proliferation, migration, and invasion of gastric cancer cells and increased the apoptosis of gastric cancer cells. The growth rate of GAS5 group slowed down and the volume of tumor decreased. miR-21 is a GAS5 target and GAS5 inhibits the proliferation of gastric cancer cells by targeting miR-21. CONCLUSION: Our research shown that overexpression of GAS5 can significantly inhibit the proliferation, migration, invasion and tumor formation of gastric cancer cells, and promote the apoptosis of gastric cancer cells, which may be related to the targeting inhibition of miR-21 expression by GAS5.

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