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1.
Int J Biol Macromol ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32061692

RESUMO

Polysaccharide is one of the essential biological macromolecules for all living things. In this study, polysaccharide from Smilacina japonica root was investigated by extraction, purification, characterization and antioxidant activity. Result showed that three kinds of pure polysaccharides labeled as SJP1-1, SJP2-1 and SJP3-1 were obtained with the characteristic of the specific optical rotation was -45°, -75°, -35°, the polysaccharide content was 97.91%, 99.05%, 89.22%, the relative average molecular weight was 1.87 × 105 Da, 1.04 × 105 Da, 1.36 × 104 Da, and the protein content was 3.78%, 4.64%, 9.32%,respectively. TLC, IR and NMR analysis showed that SJP1-1 was a heteropolysaccharide composed mainly of ß-glucose (1 → 3) residues, ß-glucose (1 → 4) residues, α-mannose (1 → 4) residues, and α-galactose (1 → 4) residues. SJP2-1 and SJP3-1 were glucose-based polysaccharides with a small amount of mannose. Scanning electron microscopy (SEM) showed that three purified polysaccharides SJP1-1, SJP2-1 and SJP3-1 presented uniform spherical shape with a diameter of 10-20 mm, whether it is uniformly dispersed particles or blocky structure surfaces, and surface smoothness. Antioxidant assay showed that these Smilacina japonica polysaccharides had strong ability to scavenge OH and DPPH, and the effect of purifying polysaccharides was greater than that of crude polysaccharides.

2.
Small ; : e1906005, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31971660

RESUMO

In this work, a phosphine-based covalent organic framework (Phos-COF-1) is successfully synthesized and employed as a template for the confined growth of broad-scope nanoparticles (NPs). Ascribed to the ordered distribution of phosphine coordination sites in the well-defined pores, various stable and well-dispersed ultrafine metal NPs including Pd, Pt, Au, and bimetallic PdAuNPs with narrow size distributions are successfully prepared as determined by transmission electron microscopy, X-ray photoelectron spectroscopy, inductively coupled plasma, and powder X-ray diffraction analyses. It is also demonstrated that the as-prepared Phos-COF-1-supported ultrafine NPs exhibit excellent catalytic activities and recyclability toward the Suzuki-Miyaura coupling reaction, reduction of nitro-phenol and 1-bromo-4-nitrobenzene, and even tandem coupling and reduction of p-nitroiodobenzene. This work will open many new possibilities for preparing COF-supported ultrafine NPs with good dispersity and stability for a broad range of applications.

3.
Vet Microbiol ; 241: 108573, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31928705

RESUMO

Caprine parainfluenza virus type 3 (CPIV3) is the one of most common causative agents of caprine respiratory infection, resulting in significant economic losses in the goat and sheep industries. However, the molecular mechanisms and host genes involved in the pathogenesis of and immunity against CPIV3 infection remain poorly understood. In this study, we used RNA-Seq to understand the responses of madin-darby bovine kidney (MDBK) cells to CPIV3 infection. A total of 261 differentially-expressed genes (DEGs) were identified in CPIV3-infected compared with mock-infected MDBK cells at 24 h post-infection (hpi). The DEGs were mainly involved in immune system processes, metabolic processes, and signal transduction. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that the most significantly enriched signaling pathways were MAPK, Wnt, PI3K-Akt, tumor necrosis factor, Toll-like receptor and ubiquitin-mediated proteolysis. STRING analysis revealed that seven interferon-stimulated genes (ISGs) were upregulated (IFI6, ISG15, OAS1Y, OAS1Z, MX1, MX2 and RSAD2) and may play a pivotal role during CPIV3 infection. Moreover, overexpression of these ISGs significantly reduced CPIV3 replication in vitro, while siRNA silencing markedly improved CPIV3 replication 24 and 48 hpi. Ours is the first study to profile the gene expression of CPIV3-infected MDBK cells. We identified seven ISGs that could be targeted in novel antiviral strategies against CPIV3.

4.
Biomaterials ; 232: 119719, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31901688

RESUMO

Tissue adhesives are commonly used in surgeries and regenerative engineering for the repair and regeneration of topical and internal wounds on tissues and organs such as skin, heart, blood vessels, and bone. However, achieving rapid crosslinking, strong wet adhesion and cohesion strengths, and minimal cytotoxicity remains a critical roadblock for clinical translation. Herein, in contrast to harsh and cytotoxic oxidants, magnesium oxide (MgO) particles were found to facilitate rapid crosslinking for injectable citrate-based mussel-inspired tissue bioadhesives synthesized by reacting citric acid, PEG-PPG-PEG diol and dopamine (iC-EPE). Our results confirmed the role of MgO particles as both crosslinkers and composite fillers to concurrently enhance bioadhesive cohesion and adhesion. iC-EPE crosslinked by MgO with/without sodium periodate (PI) exhibit enhanced mechanical strengths (1.0 Mpa < tensile strength ≤ 4.5 MPa) compared to that of iC-EPE crosslinked only by PI (~0.75 MPa), high adhesion strength (up to 125 kPa, 8 fold that of fibrin glue (~15 kPa)), tunable degradability (full degradation from <1 week to > 1 month), excellent in vitro and in vivo biocompatibility, encouraging anti-bacterial performance, and favorable wound closure efficacy. Thus, MgO crosslinked bioadhesives possess great potential for a wide range of applications in surgery and regenerative engineering.

5.
Eur J Radiol ; 124: 108840, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31981879

RESUMO

PURPOSE: To establish an accurate and reliable equation for kidney depth estimation in adult patients from different Chinese geographical regions. METHOD: This multicenter study enrolled Eastern Asian Chinese patients with abdominal PET/CT scans at 26 imaging centers from six macro-regions across China in 3 years. Age, gender, height, weight, primary disease and its extent on PET scans of the participants were collected as potential predictive factors. Kidney depth on CT, defined as the average of the vertical distances from the posterior skin to the farthest anterior and closest posterior surfaces of each kidney, was measured as the standard reference. The new kidney depth model was constructed using a multiple regression model, and its performance was compared to those of three established models by computing the absolute value of estimation errors in comparison with CT-measured kidney depth. RESULTS: A total of 2502 patients were enrolled and classified into training (n=1653) and testing (n = 849) subsets. In the training subset, two kidney depth models were constructed: Left (cm): 0.013×age+0.117×gender-0.044×height+0.087×weight+7.951, Right (cm): 0.005×age+0.013×gender-0.035×height+0.082×weight+7.266 (weight: kg, height: cm, gender = 0 if female, 1 if male). In the testing subset, one-way analysis of variance showed that the estimation errors of the new models did not significantly differ among the 6 regions. Bland-Altman analysis determined that new equations had lower estimated biases (left: 0.039 cm, right: 0.018 cm) compared with other existing models. CONCLUSION: The new equations were highly accurate for kidney depth estimation in adults from all over China, with lower estimation errors compared to other established models.

6.
Chemistry ; 26(4): 853-862, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31691394

RESUMO

The Fe-based transition metal oxides are promising anode candidates for lithium storage considering their high specific capacity, low cost, and environmental compatibility. However, the poor electron/ion conductivity and significant volume stress limit their cycle and rate performances. Furthermore, the phenomena of capacity rise and sudden decay for α-Fe2 O3 have appeared in most reports. Here, a uniform micro/nano α-Fe2 O3 nanoaggregate conformably enclosed in an ultrathin N-doped carbon network (denoted as M/N-α-Fe2 O3 @NC) is designed. The M/N porous balls combine the merits of secondary nanoparticles to shorten the Li+ transportation pathways as well as alleviating volume expansion, and primary microballs to stabilize the electrode/electrolyte interface. Furthermore, the ultrathin carbon shell favors fast electron transfer and protects the electrode from electrolyte corrosion. Therefore, the M/N-α-Fe2 O3 @NC electrode delivers an excellent reversible capacity of 901 mA h g-1 with capacity retention up to 94.0 % after 200 cycles at 0.2 A g-1 . Notably, the capacity rise does not happen during cycling. Moreover, the lithium storage mechanism is elucidated by ex situ XRD and HRTEM experiments. It is verified that the reversible phase transformation of α↔γ occurs during the first cycle, whereas only the α-Fe2 O3 phase is reversibly transformed during subsequent cycles. This study offers a simple and scalable strategy for the practical application of high-performance Fe2 O3 electrodes.

7.
ACS Appl Mater Interfaces ; 11(51): 47886-47893, 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31797668

RESUMO

The intrinsic charge-transfer property bears the primary responsibility for the sluggish redox kinetics of the common electrode materials, especially operated at low temperatures. Herein, we report the crafting of homogeneously confined Fe7Se8 nanoparticles with a well-defined graphitic carbon matrix that demonstrate a highly efficient charge-transfer system in a designed natural coral-like structure (cl-Fe7Se8@C). Notably, the intricate architecture as well as highly conductive peculiarity of C concurrently satisfy the demands of achieving fast ionic/electrical conductivities for both Li/Na-ion batteries in a wide temperature range. For example, when cl-Fe7Se8@C is employed as the anode material to assemble full batteries with the cathode of Na3V2(PO4)2O2F (NVPOF), decent capacities of 323.1 and 175.9 mA h g-1 can be acquired at temperatures of 25 and -25 °C, respectively. This work is significant for further developing potential anode materials for advanced energy storage and conversion under low-temperature conditions.

8.
Medicine (Baltimore) ; 98(51): e14222, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31860943

RESUMO

RATIONALE: Patients with gastrointestinal stromal tumors (GISTs) are often found to have liver metastases at their 1st presentation. Most patients need preoperative treatment to reduce the size of the liver metastases to increase the possibility of surgical resection. Currently, imatinib mesylate is the drug of 1st choice for preoperative treatment and sunitinib malate (SM) is seldom used. Here we report a case of GIST with liver metastases where SM was used as a preoperative treatment. PATIENT CONCERNS: A 56-year-old worker presented with intermittent abdominal pain and eating difficulties. DIAGNOSES: An enhanced computed tomography scan showed a 15 × 15 × 10 cm malignant mass in the upper abdomen, and 2 metastases (15.1 × 13.1 cm and 14.8 × 8.8 cm) in the liver. The postcaval and middle hepatic veins were compressed by the liver metastases, making radical resection very difficult. INTERVENTIONS: First the primary tumor in the jejunum was resected, and then SM was used as a preoperative treatment to reduce the size of the liver metastases to improve the possibility of surgical resection. OUTCOMES: Both liver metastases regressed considerably in size and it was then possible to perform a radical resection. LESSONS: The SM has the potential to be used as preoperative therapy for GIST with large liver metastases. This method provides a new option for the preoperative treatment of GIST with liver metastases.


Assuntos
Tumores do Estroma Gastrointestinal/tratamento farmacológico , Tumores do Estroma Gastrointestinal/patologia , Neoplasias do Jejuno/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/secundário , Sunitinibe/uso terapêutico , Dor Abdominal/diagnóstico , Dor Abdominal/etiologia , Seguimentos , Tumores do Estroma Gastrointestinal/cirurgia , Hepatectomia/métodos , Humanos , Neoplasias do Jejuno/patologia , Neoplasias do Jejuno/cirurgia , Neoplasias Hepáticas/cirurgia , Masculino , Pessoa de Meia-Idade , Terapia Neoadjuvante/métodos , Invasividade Neoplásica/patologia , Estadiamento de Neoplasias , Cuidados Pré-Operatórios/métodos , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento
9.
J Orthop Surg Res ; 14(1): 402, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31779638

RESUMO

BACKGROUND: Although supramalleolar osteotomy is the main joint-preserving method for the treatment of varus ankle osteoarthritis, it tends to be ineffective when ankle osteoarthritis presents in combination with an excessive talar tilt angle. The purpose of this study was to present a new surgical technique, supramalleolar osteotomy combined with lateral ligament reconstruction and talofibular immobilization, for the treatment of varus ankle osteoarthritis with an excessive talus tilt angle and to evaluate the clinical and radiological results. METHODS: From January 2013 to October 2016, a total of 17 patients with 17 cases of varus ankle arthritis with excessive talar tilt angles (larger than 7.3°) underwent surgical treatment using our new technique. The American Orthopaedic Foot and Ankle Society (AOFAS) clinical ankle-hindfoot scale and a visual analogue scale (VAS) were used to evaluate ankle function and pain before surgery and at the last follow-up. The medial distal tibial angle (MDTA), anterior distal tibial angle (ADTA), talar tilt angle (TTA), and hindfoot moment arm values (HMAVs) were evaluated on weight-bearing radiographs acquired preoperatively and at the last follow-up. RESULTS: The AOFAS score improved significantly from 45.8 ± 2.1 before surgery to 84.8 ± 1.8 after surgery (p < 0.001), and the VAS score decreased from 4.9 ± 0.4 to 1.1 ± 0.2 (p < 0.001). The MDTA, TTA, and HMAV changed from 80.9° ± 0.4° to 90.1° ± 0.4°, 11.7° ± 0.6° to 1.4° ± 0.3°, and 12.6 mm ± 0.8 mm to 4.2 mm ± 0.6 mm, respectively (each p < 0.001). The ADTA showed no obvious change (p = 0.370). The staging of 11 cases (65%) improved. Intramuscular vein thrombosis of the lower limbs occurred in 1 patient 1 week after surgery, and superficial infection occurred in 1 patient. CONCLUSIONS: Supramalleolar osteotomy combined with lateral ligament reconstruction and talofibular immobilization can correct the load of the weight-bearing ankle and effectively improve the ankle function. As the talar tilt angle can be significantly improved after surgery, this technique can be used for the treatment of varus ankle osteoarthritis with an excessive TTA.

10.
Arthropod Struct Dev ; 53: 100901, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31760197

RESUMO

The Asian corn borer Ostrinia furnacalis is one of the most destructive pests of maize throughout eastern Asia and the South Pacific. In the present study the fine structure of the compound eyes of adult O. furnacalis was investigated under light/dark adaptation using light and electron microscopy. The compound eyes of male and female O. furnacalis are superposition eyes with electron-lucent clear zones. The sexual differences of the compound eyes of O. furnacalis are mainly reflected in eye size rather than ommatidial ultrastructure. Each ommatidium of both sexes contains 12 retinula cells, one of which is the basal retinula cell. All the retinula cells form a centrally-fused, two-tiered rhabdom, whose distal layer passes through the clear zone and distally connects with the crystalline cone. The ultrastructural changes under light/dark conditions mainly involve the rhabdom occupation ratio to retinula cell volume in the proximal layer of the rhabdom as well as the dimensions of the subcorneal zone and the crystalline tract. Pigment movements occur within the retinula cells and primary pigment cells, but are undetectable within the secondary pigment cells. Regardless of light or dark adaptation, in other words, the pigments never migrate into the clear zone.

11.
Zootaxa ; 4608(1): zootaxa.4608.1.2, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31717159

RESUMO

Eight species of Trypetisoma Malloch, 1924 from Southwest China are described as new to science: T. (Trypaneoides) endosclerita sp. nov., T. (Tn.) jinpingensis sp. nov., T. (Tn.) menglunensis sp. nov., T. (Tn.) semicircularis sp. nov., T. (Tn.) trimaculata sp. nov., T. (Tn.) triprocessa sp. nov., T. (Tn.) xii sp. nov., T. (Trypetisoma) spinosa sp. nov., and T. (Tn.) fenestrata (de Meijere, 1910) is recorded for China for the first time. These species represent the first records for the subgenus Trypetisoma for the Oriental region. A key to the subgenera and species of genus Trypetisoma in the Oriental region is presented.


Assuntos
Dípteros , Distribuição Animal , Animais , China
12.
Sci Rep ; 9(1): 16910, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729423

RESUMO

Long non-coding RNAs (lncRNAs) act as competing endogenous RNAs (ceRNAs) to compete with microRNAs (miRNAs) in cancer occurrence and development. However, the differential expression of RNAs and their ceRNA network during the development of colon cancer (CC) remains unclear. This study was aimed at comprehensive analysis of the lncRNAs and their ceRNA networks associated with CC. Whole transcriptome sequencing was performed on colorectal and adjacent normal tissues at different pathological stages. Forty-nine lncRNAs were differently expressed between the CC tissues and their adjacent normal tissues at all stages. Aberrant expression of lncRNA CDKN2B-AS1 and lncRNA MIR4435-2HG was confirmed by TCGA database. Moreover, 14 lncRNAs were differentially expressed between early and advance stages of the tumor tissues, and 117 miRNAs were specifically expressed in stage III & IV. Weighted gene co-expression network analysis of 17105 differently expressed mRNAs revealed that the mRNAs shown in module pink, midnight blue, black, and light cyan were related to TNM and pathological stage, and that these mRNAs were enriched in cancer related functions and pathways. As DElncRNA showed a trend of change similar to that of the DEmRNA and opposite to that of DEmiRNA, ceRNA network was constructed with 3 DEmiRNAs, 5 DElncRNAs, and 130 DEmRNAs. Real time PCR revealed that expression of MEG3 was decreased in the tumor tissues belonging to stage III and IV as compared to that in stage I. Moreover, hsa-miR-324-5p was upregulated, while FGFR3, PLCB4, and IKBKB were downregulated in the tumor tissues as compared to that in the adjacent normal tissues. Thus, this study revealed differentially expressed lncRNA between different stages of CC as well as suggested that lncRNA CDKN2B-AS1, MIR4435-2HG, and MEG3 may act as diagnostic biomarkers for the development of CC.

13.
Sensors (Basel) ; 19(20)2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31614888

RESUMO

Large-scale urbanization has brought about severe ground subsidence in Kunming (China), threatening the stability of urban infrastructure. Mapping of the spatiotemporal variations of ground deformation is urgently needed, along with summarization of the causes of the subsidence over Kunming with the purpose of disaster prevention and mitigation. In this study, for the first time, a multi-temporal interferometric synthetic aperture radar (InSAR) technique with L-band Advanced Land Observation Satellite (ALOS-1) and X-band Constellation of Small Satellites for Mediterranean basin Observation (COSMO-SkyMed) data was applied to Kunming to derive the time series deformation from 2007 to 2016. The annual deformation velocity revealed two severe subsiding regions in Kunming, with a maximum subsidence of 35 mm/y. The comparison of the deformation between InSAR and leveling showed root-mean-square error (RMSE) values of about 4.5 mm for the L-band and 3.7 mm for the X-band, indicating that our results were reliable. We also found that the L-band illustrated a larger amount of subsidence than the X-band in the tested regions. This difference was mainly caused by the different synthetic aperture radar (SAR)-acquired times and imaging geometries between the L- and X-band SAR images. The vertical time series deformation over two severe subsiding regions presented an approximate linear variation with time, where the cumulative subsidence reached 209 mm during the period of 2007-2016. In view of relevant analyses, we found that the subsidence in Kunming was the result of soft soil consolidation, building load, and groundwater extraction. Our results may provide scientific evidence regarding the sound management of urban construction to mitigate potential damage to infrastructure and the environment.

14.
Cancer Biol Med ; 16(3): 606-617, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31565489

RESUMO

Objective: More than half of human glioblastomas show EGFR gene amplification and mutation, but EGFR inhibitors have not been effective in treating EGFR-positive glioblastoma patients. The mechanism behind this type of primary resistance is not well understood. The aim of this study was to investigate gefitinib resistance in glioblastoma, and explore ways to circumvent this significant clinical problem. Methods: MTT method was used to test the cell viability after EGFR-positive glioblastoma cells were treated with indicated drugs; real-time quantitative PCR method was included to detect the TNFα mRNA levels in glioma tissues and cell lines. ELISA was introduced to measure the TNFα protein levels in cell culture supernatant of glioblastoma cells treated with gefitinib. Western blot was used to detect the activity change of intracellular kinases in drug-treated glioblastoma cells. Two mouse xenograft tumor models were carried out to evaluate the in vivo effects of a combination of EGFR and TNFα inhibitors. Results: We found that glioblastoma resistance to gefitinib may be mediated by an adaptive pro-survival TNFα-JNK-Axl signaling axis, and that high TNFα levels in the glioblastoma microenvironment may further intensify primary resistance. A combination of the TNFα-specific small-molecule inhibitor C87 and gefitinib significantly enhanced the sensitivity of glioblastoma cells to gefitinib in vitro and in vivo. Conclusions: Our findings provide a possible explanation for the primary resistance of glioblastoma to EGFR inhibitors and suggest that dual blockade of TNFα and EGFR may be a viable therapeutic strategy for the treatment of patients with chemotherapy-refractory advanced glioblastoma.

15.
Med Sci Monit ; 25: 7073-7078, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31539366

RESUMO

BACKGROUND Sorbitol-aldose reductase (polyol) pathway genes have been strongly linked to diabetic retinopathy. Polymorphisms in these genes may affect their functions and influence the risk of retinopathy. In this work, we investigated the influence of the rs759853 polymorphism of ALR2 gene and rs2055858 and rs3759890 polymorphisms of SDH gene on risk of diabetic retinopathy among Han Chinese. MATERIAL AND METHODS We included 3,000 subjects in our study, of which 1,500 were patients with diabetic retinopathy and 1,500 were controls without the said condition. Among the cases, 750 had the non-proliferative diabetic retinopathy (NPDR) and 750 had proliferative diabetic retinopathy (PDR). The polymorphisms were genotyped using established methods and logistic regression analysis was used to determine whether the polymorphisms were associated with risk of diabetic retinopathy. RESULTS We found that variants of ALR2 rs759853 polymorphism were significantly associated with an increased risk of diabetic retinopathy, whereas variants of SDH rs2055858 polymorphism were significantly associated with a lower risk. For the former, an odds ratio (OR) of 1.46 were noted for the heterozygous genotype (95% CI=1.25-1.70, P<0.01) and the homozygous variant genotype (OR=1.90, 95% CI=1.40-2.60, P<0.01). For SDH rs2055858 polymorphism, an OR of 0.51 (95% CI=0.43-0.61, P<0.01) and 0.34 (95% CI=0.28-0.42, P<0.01) was observed for heterozygous and homozygous variant genotype respectively. Subgroup analysis based on NPDR and PDR showed a similar finding as the combined results. CONCLUSIONS ALR2 rs759853 and SDH rs2055858 polymorphisms were respectively associated with a higher and lower risk of diabetic retinopathy.

16.
Cell Mol Neurobiol ; 39(8): 1207-1215, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31342232

RESUMO

The KDEL (Lys-Asp-Glu-Leu) receptors (KDELRs), proteins with seven transmembrane domains, are primarily responsible for endoplasmic reticulum (ER) homeostasis. Recent studies have found additional function of KDELRs in growth, cellular secretory traffic, immune response, and autophagy; however, its role in tumorigenesis is still poorly understood. Here, we showed that KDELR2 is highly expressed in glioblastoma (GBM) tissues. Reviewing the expression of KDELR2 in TCGA and REMBRANDT database, we found that higher expression of KDELR2 is associated with shorter survival of GBM patients. We explored the effect of KDELR2 on tumorigenesis in GBM cells and animal model (nude mice), and identified KDELR2 as oncogene promoting cell proliferation. Additionally, KDELR2 expression in GBM cells correlated positively with HIF1alpha (HIF1α) expression, and we demonstrated by ChIP-qPCR and luciferase reporter assay that the upstream region of the KDELR2 gene is directly targeted by HIF1alpha. Taken together, our data suggest that KDELR2 is a target gene downstream of HIF1-alpha driving the malignancy of GBM and could eventually serve as a therapeutic target for the treatment of GBM patients.


Assuntos
Neoplasias Encefálicas/patologia , Carcinogênese/patologia , Glioblastoma/patologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Animais , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Carcinogênese/genética , Carcinogênese/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Glioblastoma/genética , Glioblastoma/metabolismo , Humanos , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Camundongos Nus , Análise de Sobrevida , Transcrição Genética , Regulação para Cima/genética , Proteínas de Transporte Vesicular/genética
17.
Microb Pathog ; 134: 103569, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31163247

RESUMO

Caprine parainfluenza virus type 3 (CPIV3) is one of the important viral respiratory tract agents in goats. The pathogenicity of CPIV3 has been examined in goats but it has not been explored in other laboratory animals. In the present study, an experimental infection of guinea pigs with CPIV3 was performed. The virus-inoculated guinea pigs displayed clinical signs related to the respiratory disease at 2-12 days post inoculation (dpi). Five infected guinea pigs died during 2 and 7 dpi. Apparent gross pneumonic lesions including consolidation and congestion in one or more lung lobes were observed in necropsied and dead animals. Histo-pathological changes in lungs including expansions of the alveolar interstitium, congestion, macrophage infiltration and compensatory emphysema were also observed. Virus was detectable at 2-10 dpi, 2-10 dpi and 2-7 dpi, as detected by virus isolation, real-time RT-PCR and immunohistochemistry staining, respectively. Viremia was also confirmed after CPIV3 infection during 3-7 dpi. The severe pathological lesions and highest viral load were observed before 7 dpi. Viral specific hemagglutination inhibition and neutralizing antibodies were produced from 7 dpi and 10 dpi, respectively, which related to the clearance of virus. The results present here indicated that guinea pig could be an ideal laboratory animal model for CPIV3 studies in the future.


Assuntos
Doenças das Cabras/virologia , Vírus da Parainfluenza 3 Humana/patogenicidade , Infecções por Respirovirus/veterinária , Animais , Anticorpos Neutralizantes , Anticorpos Antivirais , Modelos Animais de Doenças , Feminino , Cabras , Cobaias , Pulmão/patologia , Pulmão/virologia , Reação em Cadeia da Polimerase em Tempo Real , Infecções por Respirovirus/imunologia , Carga Viral , Viremia , Virulência
18.
Theor Appl Genet ; 132(9): 2579-2589, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31187154

RESUMO

KEY MESSAGE: qhkw5-3, a major QTL for kernel weight in maize, was mapped to an interval of 125.3 kb between the InDel markers InYM20 and InYM36, and the candidate genes were analysed. Yield, of which kernel weight is a major component, is the primary trait of interest in maize breeding programmes. In our previous study, a major QTL (named qhkw5-3), which controls hundred-kernel weight, was identified and mapped to the interval between simple sequence repeat (SSR) markers SYM033 and SYM108 on chromosome 5, using an F2:3 population derived from a cross between the maize inbred line Zheng58 and the single-segment substitution line Z22. In order to fine map qhkw5-3, a larger BC1F1 segregating population of 14,759 seeds, derived from a (Z22 × Zheng58) × Z22 cross, was screened using the SSR markers SYM036 and SYM119. Forty genotypes with donor chromosomal fragments of different lengths were obtained. Progeny testing results indicated that qhkw5-3 was mapped to an interval of 442.6 kb between the SSR markers SYM077 and SYM084. Overlap mapping results, based on seven homozygous recombinant lines, showed that qhkw5-3 was narrowed down to an interval of 125.3 kb between the InDel markers InYM20 and InYM36. Within this interval, six candidate genes were analysed using qRT-PCR. The results of this study lay the foundations for cloning and functional analysis of qhkw5-3 and will contribute to advancing our knowledge of the genetic basis of yield traits in maize.

19.
BMC Genomics ; 20(1): 516, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31226933

RESUMO

BACKGROUND: Bovine viral diarrhea virus (BVDV) is an economically important viral pathogen of domestic and wild ruminants. Apart from cattle, small ruminants (goats and sheep) are also the susceptible hosts for BVDV. BVDV infection could interfere both of the innate and adaptive immunity of the host, while the genes and mechanisms responsible for these effects have not yet been fully understood. Peripheral blood mononuclear cells (PBMCs) play a pivotal role in the immune responses to viral infection, and these cells were the target of BVDV infection. In the present study, the transcriptome of goat peripheral blood mononuclear cells (PBMCs) infected with BVDV-2 was explored by using RNA-Seq technology. RESULTS: Goat PBMCs were successfully infected by BVDV-2, as determined by RT-PCR and quantitative real-time RT-PCR (qRT-PCR). RNA-Seq analysis results at 12 h post-infection (hpi) revealed 499 differentially expressed genes (DEGs, fold-change ≥ ± 2, p < 0.05) between infected and mock-infected PBMCs. Of these genes, 97 were up-regulated and the remaining 352 genes were down-regulated. The identified DEGs were found to be significantly enriched for locomotion/ localization, immune response, inflammatory response, defense response, regulation of cytokine production, etc., under GO enrichment analysis. Cytokine-cytokine receptor interaction, TNF signaling pathway, chemokine signaling pathway, etc., were found to be significantly enriched in KEGG pathway database. Protein-protein interaction (PPI) network analysis indicated most of the DEGs related to innate or adaptive immune responses, inflammatory response, and cytokine/chemokine-mediated signaling pathway. TNF, IL-6, IL-10, IL-12B, GM-CSF, ICAM1, EDN1, CCL5, CCL20, CXCL10, CCL2, MAPK11, MAPK13, CSF1R and LRRK1 were located in the core of the network and highly connected with other DGEs. CONCLUSIONS: BVDV-2 infection of goat PBMCs causes the transcription changes of a series of DEGs related to host immune responses, including inflammation, defense response, cell locomotion, cytokine/chemokine-mediated signaling, etc. The results will be useful for exploring and further understanding the host responses to BVDV-2 infection in goats.


Assuntos
Vírus da Diarreia Viral Bovina Tipo 2/genética , Doenças das Cabras/imunologia , Doenças das Cabras/virologia , Infecções por Pestivirus/veterinária , Animais , Vírus da Diarreia Viral Bovina Tipo 2/imunologia , Perfilação da Expressão Gênica , Regulação Viral da Expressão Gênica , Doenças das Cabras/genética , Cabras , Imunidade/genética , Leucócitos Mononucleares/virologia , Infecções por Pestivirus/genética , Infecções por Pestivirus/imunologia , Análise de Sequência de RNA , Replicação Viral
20.
BMC Vet Res ; 15(1): 151, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31101113

RESUMO

BACKGROUND: Caprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis. RESULTS: Quantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value < 0.05 and fold change > 1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data. CONCLUSION: To our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.


Assuntos
Proteínas de Choque Térmico/metabolismo , Proteômica , Infecções por Respirovirus/veterinária , Respirovirus/fisiologia , Animais , Western Blotting , Bovinos , Linhagem Celular , Cromatografia Líquida , Perfilação da Expressão Gênica , Proteínas de Choque Térmico/genética , Rim/virologia , Reação em Cadeia da Polimerase em Tempo Real , Respirovirus/genética , Infecções por Respirovirus/genética , Infecções por Respirovirus/metabolismo , Espectrometria de Massas em Tandem , Replicação Viral/fisiologia
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