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1.
Food Chem ; 371: 131201, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34598116

RESUMO

Hydrophilic constituents are significant for the taste and nutrition of tea, but their simultaneous quantification remains challenging due to the lack of efficient methods. Based on the hydrophilic interaction chromatography coupled with triple quadrupole-tandem mass spectrometry, this work developed and validated an efficient (8.5 min per run), sensitive (LOQ: 0.002-0.493 µg/mL) and accurate method. This method was successfully used to determine the contents of 45 hydrophilic constituents in Yunnan large-leaf tea. Umami amino acids and umami-enhanced nucleotides generally exhibited higher content in green tea and Pu-erh raw tea. By contrast, a few number of amino acids (e.g., proline and γ-aminobutyric acid) and most alkaloids and nucleosides showed significantly higher contents in black tea or Pu-erh ripen tea. By performing the orthogonal partial least squares discriminant analysis, classification models for distinguishing four types of tea, and green tea from Pu-erh raw tea were established.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34622541

RESUMO

The delivery of protein into mammalian cells enables the dissection and manipulation of biological process, while this potency is challenged by lacking efficient protein delivery tool and the way to monitor its intracellular trafficking. Herein, we report that the hierarchical self-assembly of tetraphenylethylene (TPE)-featured metal-organic cages (MOCs) and ß-cyclodextrin-conjugated polyethylenimine can generate fluorescent supramolecular nanoparticles (FSNPs) to deliver protein into neural cells, a hard-to-transfect cell line using conventional strategy. Further, the aggregation-induced emission (AIE) feature of TPE enabled the fluorescent monitoring of cytosolic protein release. It is found that FSNPs can deliver and release protein into cytosol for subcellular targeting as fast as 18 h post-delivery. Moreover, the delivery of molecular chaperone DJ-1 using FSNPs activates MAPK/ERK signaling of neural cells to protect cells from oxidative stress. Together, our strategy provides a powerful tool for neural cell protein delivery and deciphering cell signaling for developing neurodegenerative disease treatment.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34613691

RESUMO

Due to the high theoretical specific capacity and low cost, FeF3·0.33H2O has become one of the potential choices of cathode materials for sodium-ion batteries. However, the poor intrinsic conductivity limits its practical applications. Herein, the atomic substitution is used to improve its intrinsic conductivity. The first-principles calculation results show that Cr3+ doping can reduce the band gap of FeF3·0.33H2O to improve its intrinsic conductivity. The discharge specific capacity of Fe0.95Cr0.05F3·0.33H2O with a narrowest band gap is 194.02 mA h/g at 0.1 C within the range of 1.4-4.0 V, which is higher than that of FeF3·0.33H2O (136.47 mA h/g). Using the electrochemical impedance spectroscopy and galvanostatic intermittent titration technique tests, it is found that Rct of Fe0.95Cr0.05F3·0.33H2O is reduced and DNa+ is almost unchanged, as compared to FeF3·0.33H2O.

4.
Bioengineered ; 12(1): 6902-6912, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34516334

RESUMO

Hepatocellular carcinoma (HCC) represents a type of lethal cancer in the world and its treatment options produce limited and unsatisfactory effectiveness. MicroRNAs (miRNAs) that play critical roles in tumorigenesis have shown promising clinical therapeutic potential. Here, we reported that miRNA-495 (miR-495) plays important roles in inhibiting HCC cell growth via its regulation of cell-cycle progression as well as senescence. MiR-495 showed low levels in human HCC tissues and cells. Overexpressing miR-495 in HCC cells caused strong cell growth inhibition, which results from cell-cycle arrest and senescence. CTRP3 functioned as a possible target of miR-495 in HCC cells by bioinformatics prediction and biological assay. By inhibiting the expression of CTRP3 with siRNA, HCC cells also showed similar growth inhibition as miR-495 overexpression. The re-expression of CTRP3 in HCC cells with high-level miR-495 abolished miR-495 and caused cell growth inhibition. These results strongly suggested that CTRP3 was the functional target that weakened the effects of miR-495 in HCC cells. The in vivo experiment demonstrated miR-495 overexpression had great therapeutic effects on HCC in xenograft. Above all, this research revealed that miR-495 is essential in suppressing HCC growth, and its application serves as a promising strategy for HCC treatment.

5.
Biomater Sci ; 9(20): 6889-6902, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34519743

RESUMO

Biofilm-related bacterial infections are extremely resistant to antibiotics, mainly due to the impermeability of the intensive matrices, which allow the bacteria to survive antibiotic treatment. Herein, step-by-step dual stimuli-responsive azithromycin-loaded nanoparticles (CM/AZM@Tyr) was constructed for efficient biofilm eradication. CM/AZM@Tyr was prepared by the self-assembly of poly(ε-caprolactone)-polyethylene glycol-polyethylenimine (PCL-PEG-PEI) into cationic micelles and simultaneously encapsulated AZM into the hydrophobic core, which is further bound with cis-aconityl-D-tyrosine (CA-Tyr) through electrostatic interaction. Upon initial penetration, CM/AZM@Tyr could show step-by-step dual-response to the microenvironment of biofilms. Firstly, the CA-Tyr shell rapidly responded to the acidic microenvironment and released D-Tyr to disassemble the biofilm mass. Then, the exposed cationic CM/AZM micelles could bind firmly to the negatively-charged bacteria cell membrane. With the enzymolysis of the PCL core, the rapidly releasing AZM could kill the bacteria over the depth of biofilms. Massive accumulation was observed in the infected lungs of biofilms-associated lung infection mice after the i.v. injection of CM/Cy5.5@Tyr under the 3D mode of the in vivo Imaging System. Reduced bacterial burden and alleviated fibrosis in the infected lungs were also obtained after treatment with CM/AZM@Tyr mainly due to its intensive penetration in the biofilm and the orderly release of the biofilm dispersant and antimicrobial agents. In summary, this research developed an effective strategy for the treatment of blood-accessible biofilm-induced infections.


Assuntos
Biofilmes , Nanopartículas , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Azitromicina , Bactérias , Camundongos
6.
Artigo em Inglês | MEDLINE | ID: mdl-34585486

RESUMO

MXenes have attracted great interests as supercapacitors due to their metallic conductivity, high density, and hydrophilic nature. Herein we report Ti3C2-Cu/Co hybrids via molten salt etching in which the existence of metal atoms and their interactions with MXene via surficial O atoms were elucidated by XAFS for the first time. The electrochemical investigation of Ti3C2-Cu electrode demonstrated the pseudocapacitive contribution of Cu and a splendid specific capacitance of 885.0 F g-1 at 0.5 A g-1 in 1.0 M H2SO4. Symmetric supercapacitor Ti3C2-Cu//Ti3C2-Cu was demonstrated with operating voltage of 1.6 V, areal capacitance of 290.5 mF cm-2 at 1 mA cm-2, and stability over 10000 cycles. It delivered an areal energy density of 103.3 µWh cm-2 at power density of 0.8 mW cm-2, based on which a supercapacitor pouch was fabricated. It provides deeper insights into the molten salt mechanism and strategies for designing MXene-based materials for electrochemical energy storage.

7.
Front Immunol ; 12: 715464, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34539645

RESUMO

The mutants resulted from the ongoing SARS-CoV-2 epidemic have showed resistance to antibody neutralization and vaccine-induced immune response. The present study isolated and identified two novel SARS-CoV-2 neutralizing antibodies (nAbs) from convalescent COVID-19 patients. These two nAbs (XG81 and XG83) were then systemically compared with nine nAbs that were reconstructed by using published data, and revealed that, even though these two nAbs shared targeting epitopes on spike protein, they were different from any of the nine nAbs. Compared with XG81, XG83 exhibited a higher RBD binding affinity and neutralization potency against wild-typed pseudovirus, variant pseudoviruses with mutated spike proteins, such as D614G, E484Q, and A475V, as well as the authentic SARS-CoV-2 virus. To explore potential broadly neutralizing antibodies, heavy and light chains from all 18 nAbs (16 published nAbs, XG81 and XG83) were cross-recombined, and some of the functional antibodies were screened and studied for RBD binding affinity, and neutralizing activity against pseudovirus and the authentic SARS-CoV-2 virus. The results demonstrated that several recombined antibodies had a more potent neutralization activity against variant pseudoviruses compared with the originally paired Abs. Taken together, the novel neutralizing antibodies identified in this study are a likely valuable addition to candidate antibody drugs for the development of clinical therapeutic agents against SARS-CoV-2 to minimize mutational escape.


Assuntos
Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Anticorpos Amplamente Neutralizantes/imunologia , Anticorpos Amplamente Neutralizantes/uso terapêutico , SARS-CoV-2/imunologia , Glicoproteína da Espícula de Coronavírus/imunologia , Anticorpos Neutralizantes/genética , Anticorpos Neutralizantes/uso terapêutico , Anticorpos Antivirais/genética , Anticorpos Antivirais/uso terapêutico , Afinidade de Anticorpos/imunologia , Linfócitos B/imunologia , Anticorpos Amplamente Neutralizantes/genética , COVID-19/imunologia , COVID-19/terapia , Linhagem Celular , Epitopos/imunologia , Humanos , Imunoterapia/métodos , Testes de Neutralização , SARS-CoV-2/efeitos dos fármacos
8.
Adv Mater ; : e2105163, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34554610

RESUMO

Metal-organic frameworks (MOFs), which consist of central metal nodes and organic linkers, constitute a fast growing class of crystalline porous materials with excellent application potential. Herein, a series of Mn-based multimetallic MOF (bimetallic and trimetallic MIL-100) nano-octahedra are prepared by a facile one-pot synthetic strategy. The types and proportions of the incorporated elements can be tuned while retaining the original topological structure. The introduction of other metal ions is verified at the atomic level by combining X-ray absorption fine structure experiments and theoretical calculations. Furthermore, these multimetallic Mn-based MIL-100 nano-octahedra are utilized as sulfur hosts to prepare cathodes for Li-S batteries. The MnNi-MIL-100@S cathode exhibits the best Li-S battery performance among all reported MIL-100@S composite cathode materials, with a reversible capacity of ≈708.8 mAh g-1 after 200 cycles. The synthetic strategy described herein is utilized to incorporate metal ions into the MOF architecture, of which the parent monometallic MOF nano-octahedra cannot be prepared directly, thus rationally generating novel multimetallic MOFs. Importantly, the strategy also allows for the general synthesis and study of various micro-/nanoscale MOFs in the energy storage field.

9.
Front Immunol ; 12: 693775, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34484190

RESUMO

Small number of SARS-CoV-2 epidemic lineages did not efficiently exhibit a neutralization profile, while single amino acid mutation in the spike protein has not been confirmed in altering viral antigenicity resulting in immune escape. To identify crucial mutations in spike protein that escape humoral immune response, we evaluated the cross-neutralization of convalescent plasmas and RBD-specific monoclonal antibodies (mAbs) against various spike protein-based pseudoviruses. Three of 24 SARS-CoV-2 pseudoviruses containing different mutations in spike protein, including D614G, A475V, and E484Q, consistently showed an altered sensitivity to neutralization by convalescent plasmas. A475V and E484Q mutants are highly resistant to neutralization by mAb B38 and 2-4, suggesting that some crucial mutations in spike protein might evolve SARS-CoV-2 variants capable of escaping humoral immune response.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/imunologia , Mutação , SARS-CoV-2/genética , SARS-CoV-2/imunologia , Glicoproteína da Espícula de Coronavírus/genética , Glicoproteína da Espícula de Coronavírus/imunologia , Substituição de Aminoácidos/genética , Substituição de Aminoácidos/imunologia , Anticorpos Neutralizantes/imunologia , Convalescença , Humanos , Evasão da Resposta Imune , Imunidade Humoral , Testes de Neutralização , Ligação Proteica
10.
J Colloid Interface Sci ; 606(Pt 1): 784-792, 2021 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-34419817

RESUMO

Silicon sub-oxides (SiOx) are increasingly becoming a prospective anode material for lithium-ion batteries (LIBs). Nevertheless, inferior electrical conductivity and drastic volume fluctuation upon cycling significantly hamper the electrochemical performance of SiOx. In this work, rice husks (RHs)-derived pitaya-like SiOx/nitrogen-doped carbon (SNC) superstructures have been prepared by a simple electrospray-carbonization approach. SiOx nanoparticles (NPs) are well-dispersed in a spherical nitrogen-doped carbon (NC) matrix. The carbon frameworks discourage the aggregation of SiOx NPs, facilitating the kinetics for ion diffusion and charge transfer, and maintaining structural stability upon cycling, thus bringing about improved electrochemical performance. When the optimized SNC superstructures with SiOx content of 64.3% are utilized as LIBs anodes, a stable specific capacity of 622.8 mA h g-1 after 100 cycles at 0.1 A g-1, and an excellent long cycle performance of 190.1 mA h g-1 after 5000 cycles at 5 A g-1 are obtained. This effective and universal synthetic strategy for fabricating controllable superstructures offers insights into the development of high-performance LIBs.

11.
Angew Chem Int Ed Engl ; 60(41): 22315-22321, 2021 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-34382314

RESUMO

Hydrogen-bonded organic frameworks (HOFs) are porous materials with great potential for biological applications. The self-assembly of HOFs and biomacromolecules, however, is challenging. We report herein the self-assembly of nanoscale HOFs (nHOFs) to encapsulate protein for intracellular biocatalysis. The self-assembly of tetrakis(4-amidiniumphenyl)methane and azobenzenedicarboxylate can encapsulate protein in situ to form protein@nHOFs under mild conditions. This strategy is applicable to proteins with different surface charge and molecular weight, showing a high protein encapsulation efficiency and minimal effect on protein activity. A cellular delivery study shows that the protein@TA-HOFs can efficiently enter cells and retain enzyme activity for biochemical catalysis in living cells for neuroprotection. Our strategy paves new avenues for interfacing nHOFs with biological settings and sheds light on expanding nHOFs as a platform for biomacromolecule delivery and disease treatment.

12.
Curr Med Sci ; 41(4): 788-802, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34403105

RESUMO

OBJECTIVE: Antipsychotics, in particular olanzapine, are first-line medications for schizophrenia. The prefrontal cortex (PFC) is an important region for antipsychotics' therapeutic effects. The PFC inflammatory and immune pathways are associated with schizophrenia pathogenesis. However, the effect of antipsychotics on the inflammatory and immune pathways in the PFC remains unclear. We aimed to examined the time-dependent effect of olanzapine on inflammatory and immune markers in the PFC of rats. Since the inflammatory and immune pathways are related to endoplasmic reticulum (ER) stress, we further investigated whether or not olanzapine-induced inflammation and immune responses were related to ER stress. METHODS: Expression of pro-inflammatory markers including IkappaB kinase ß (IKKß), nuclear factor kappa B (NFκB), tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) and IL-1ß, and immune-related proteins including inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2) and cluster of differentiation 14 (CD14) were examined by Western blotting. RESULTS: Olanzapine treatments for 1, 8 and 36 days significantly activated the inflammatory IKKß/NFκB signaling, and increased the expression of TNF-α, IL-6, IL-1ß and immune-related proteins such as iNOS, TLR4 and CD14. Olanzapine treatment for 1 day, 8 and 36 days also induced ER stress in the PFC. Co-treatment with an ER stress inhibitor, 4-phenylbutyrate, inhibited olanzapine-induced inflammation and the immune response in the PFC. CONCLUSION: These results suggested olanzapine exposure could be a factor that induces central inflammation and immunological abnormities in schizophrenia subjects. Olanzapine induces PFC inflammation and immune response, possibly via activating ER stress signaling.

13.
Biomater Sci ; 2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34378567

RESUMO

The discovery of CRISPR/Cas9 genome-editing technology enables the precise manipulation of mammalian DNA sequences for treating genetic disorders. Despite its high efficiency for genome editing, the introduction of CRISPR/Cas9 machinery, which is composed of Cas9 nuclease protein and guide RNA, into cells challenges its clinical translation potential. Therefore, the intracellular delivery of genome-editing machinery determines the efficacy of gene manipulation via the CRISPR/Cas9 technology. Recently, metallosupramolecules including metal-organic frameworks (MOFs) and metal-organic cages (MOCs) have been designed to selfassemble with Cas9 nuclease and guide RNA for CRISPR/Cas9 delivery and genome editing. Herein, we review the most recent advances and strategies of constructing metallosupramolecules for CRISPR/Cas9 delivery. In particular, we discuss nanoscale MOFs and MOCs that could be assembled and regulated by the intracellular environment for the spatiotemporal delivery of genome editing machinery. We also provide a perspective view of the future development of metallosupramolecules for genome editing and gene therapy in vivo.

14.
J Food Sci ; 86(9): 4223-4243, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34383327

RESUMO

Bamboo(Phyllostachys edulis) shoot was reported to be rich in phenolics. In the present study, free phenolics, conjugated phenolics, and insoluble-bound phenolics of oven-drying and freeze-drying bamboo shoot tips were extracted and separated, of which total phenolic content (TPC), total flavonoid content (TFC), and their antioxidant activities were determined. Phenolics of different binding forms were qualitatively analyzed using HPLC-ESI-QqQ-MS. A total of 22, 41, and 28 compounds were confirmed or tentatively identified in free, conjugated, and insoluble-bound phenolic extraction, respectively. The majority of the identified compounds were organic acids and phenolic acids. Oven-drying samples exhibited higher TPC (10.53-24.92 mg GAE/100 g DW) and TFC (5.80-33.27 mg CE/100 g DW) values, and stronger antioxidant activities (DPPH, ABTS, and FRAP) than freeze-drying (TPC: 1.67-15.28 mg GAE/100 g DW, TFC: 1.43-29.05 mg CE/100 g DW). Insoluble-bound phenolics were the major contributor to the total antioxidant activity. The present study investigated the phenolics composition and antioxidant activities of different binding forms in bamboo shoot tip comprehensively, and provided available information for their high-value deep-processing.


Assuntos
Antioxidantes , Manipulação de Alimentos , Liofilização , Fenóis , Extratos Vegetais , Poaceae , Antioxidantes/química , Flavonoides/análise , Manipulação de Alimentos/métodos , Manipulação de Alimentos/normas , Alimentos em Conserva/análise , Fenóis/análise , Fenóis/química , Extratos Vegetais/química , Poaceae/química
15.
Biomaterials ; 276: 121038, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34339925

RESUMO

Macrophage has been gradually recognized as a central regulator in tissue regeneration, and the study of how macrophage mediates biomaterials-induced bone regeneration through immunomodulatory pathway becomes popular. However, the current understanding on the roles of different macrophage phenotypes in regulating bone tissue regeneration remains controversial. In this study, we demonstrate that sequential infiltration of heterogeneous phenotypes of macrophages triggered by bio-metal ions effectively facilitates bone healing in bone defect. Indeed, M1 macrophages promote the recruitment and early commitment of osteogenic and angiogenic progenitors, while M2 macrophages and osteoclasts support the deposition and mineralization of the bone matrix, as well as the maturation of blood vessels. Moreover, we have identified a group of bone biomaterial-related multinucleated cells that behave similarly to M2 macrophages with wound-healing features rather than participate in the bone resorption cascade similarly to osteoclasts. Our study shows how sequential activation of macrophage-osteoclast lineage contribute to a highly orchestrated immune response in the bone tissue microenvironment around biomaterials to regulate the complex biological process of bone healing. Therefore, we believe that the temporal activation pattern of heterogeneous macrophage phenotypes should be considered when the next generation of biomaterials for bone regeneration is engineered.


Assuntos
Materiais Biocompatíveis , Regeneração Óssea , Ativação de Macrófagos , Macrófagos , Osteogênese , Fenótipo
16.
Plant Dis ; 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34328361

RESUMO

Xanthomonas citri pv. citri (Xcc) is the causative agent of citrus canker, one of the most devastating disease threatening global citrus production. Here, we present the genome sequence of Xcc strain GD82 from a formalin-fixed citrus leaf specimen showing canker lesions collected at 1982 in Guangdong Province, China. The GD82 genome comprised of 5,197,217 bp with G+C content of 64.8 %, along with four circular plasmids, pXAC33 (32,377 bp), pXAC64 (63,972 bp), pXAC47 (47,810 bp) and pGD82.1 (219,560 bp). This is the oldest Xcc genome from historical citrus canker specimens in China, which will enrich the current Xcc genome database and facilitate genomic evolution research of Xcc.

17.
Commun Biol ; 4(1): 803, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34211094

RESUMO

Polyamine detection and depletion have been extensively investigated for cancer prevention and treatment. However, the therapeutic efficacy is far from satisfactory, mainly due to a polyamine compensation mechanism from the systemic circulation in the tumor environment. Herein, we explore a new solution for improving polyamine detection as well as a possible consumption therapy based on a new photosensitizer that can efficiently consume polyamines via an irreversible chemical reaction. The new photosensitizer is pyrrolopyrroleaza-BODIPY pyridinium salt (PPAB-PyS) nanoparticles that can react with the over-expressed polyamine in cancer cells and produce two photosensitizers with enhanced phototoxicity on cancer destruction. Meanwhile, PPAB-PyS nanoparticles provide a simultaneous ratiometric fluorescence imaging of intracellular polyamine. This combination polyamine consumption with a chemical reaction provides a new modality to enable polyamine detection along with photodynamic therapy as well as a putative depletion of polyamines for cancer treatment and prevention.


Assuntos
Poliaminas Biogênicas/análise , Nanopartículas/química , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Poliaminas Biogênicas/química , Linhagem Celular Tumoral , Humanos , Neoplasias/química , Neoplasias/prevenção & controle , Imagem Óptica
18.
Photodiagnosis Photodyn Ther ; 35: 102429, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34237475

RESUMO

Black phosphorus quantum dots(BPQDs) have shown a good application prospect in the field of tumor therapy due to their photoelectric effect and good biodegradability. Due to the active endocytosis and fast metabolic efficiency of tumor cells, BPQDs are easy to be absorbed by tumor cells. However, this does not guarantee that BPQDs will be completely targeted to tumor cells, and normal cells will also absorb BPQDs. Because the cell membrane is negatively charged, BPQDs are also negatively charged and are not easily absorbed by cells under the action of electrostatic repulsion. Surface pegylation is the most common modification method of black phosphorus at present. However, surface pegylation can reduce the uptake of BPQDs by tumor cells. Positive PEG is also easy to be recognized and swallowed by the reticuloendothelial system. The inherent instability and poor tumor targeting of BPQDs under physiological conditions limit further research and clinical application. For this purpose, we selected cationic polymer polyethylenimine (PEI) to modify BPQDs and then added RGD peptides targeting tumor cells. An outer layer of negatively charged PEG+DMMA makes the nanosystem more stable . In the acidic environment of the tumor, the PEG layer has a charge reversal, and the positively charged PEI and the RGD polypeptide BPQDs targeted by the tumor cells are released into the tumor cells. It provides a new method for efficiently and accurately transporting BPQDs, a novel photosensitive nanomaterial, into tumor cells for photodynamic therapy.


Assuntos
Fotoquimioterapia , Pontos Quânticos , Concentração de Íons de Hidrogênio , Fósforo , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes
19.
Mol Biol Evol ; 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34329477

RESUMO

Domestication and breeding have reshaped the genomic architecture of chicken, but the retention and loss of genomic elements during these evolutionary processes remain unclear. We present the first chicken pan-genome constructed using 664 individuals, which identified an additional ∼66.5 Mb sequences that are absent from the reference genome (GRCg6a). The constructed pan-genome encoded 20,491 predicated protein-coding genes, of which higher expression level are observed in conserved genes relative to dispensable genes. Presence/absence variation (PAV) analyses demonstrated that gene PAV in chicken was shaped by selection, genetic drift, and hybridization. PAV-based GWAS identified numerous candidate mutations related to growth, carcass composition, meat quality, or physiological traits. Among them, a deletion in the promoter region of IGF2BP1 affecting chicken body size is reported, which is supported by functional studies and extra samples. This is the first time to report the causal variant of chicken body size QTL located at chromosome 27 which was repeatedly reported. Therefore, the chicken pan-genome is a useful resource for biological discovery and breeding. It improves our understanding of chicken genome diversity and provides materials to unveil the evolution history of chicken domestication.

20.
J Colloid Interface Sci ; 602: 251-260, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34126502

RESUMO

It is still challenging to construct stable 3D energy storage materials at the nanoscale by precise pore structure control and reasonable surface modification. Herein, a novel interwoven porous Co(CO3)0.35Cl0.20(OH)1.10 (CoCCHH)-CoSe heterogeneous-tube @PEDOT:PSS 3D foam with abundant active sites is presented as supercapacitor electrodes. The electrochemical results indicated that the pore structure provides ample space for redox reaction, and increases the number of ion transport channels. Besides, rational surface modification brings about sufficient active sites for redox reaction. The stable, porous PEDOT:PSS foam with a 3D elastic frame exhibited excellent electrical conductivity. Thus, the CoCCHH-CoSe@PEDOT:PSS foam possessed excellent specific capacitance and energy density, due to the synergistic effect of the unique 3D structure and surface defects. The home-made supercapacitor with CoCCHH-CoSe@PEDOT:PSS foam as cathode materials showed high specific capacitance (440.6F g-1 at 1 A g-1) and excellent energy density (137.7 Wh kg-1). This work provides a valuable strategy to develop potential materials for electrochemical energy storage.

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