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1.
Artigo em Inglês | MEDLINE | ID: mdl-33417553

RESUMO

Deep face recognition has achieved great success due to large-scale training databases and rapidly developing loss functions. The existing algorithms devote to realizing an ideal idea: minimizing the intra-class distance and maximizing the inter-class distance. However, they may neglect that there are also low quality training images which should not be optimized in this strict way. Considering the imperfection of training databases, we propose that intra-class and inter-class objectives can be optimized in a moderate way to mitigate overfitting problem, and further propose a novel loss function, named sigmoid-constrained hypersphere loss (SFace). Specifically, SFace imposes intra-class and inter-class constraints on a hypersphere manifold, which are controlled by two sigmoid gradient re-scale functions respectively. The sigmoid curves precisely re-scale the intra-class and inter-class gradients so that training samples can be optimized to some degree. Therefore, SFace can make a better balance between decreasing the intra-class distances for clean examples and preventing overfitting to the label noise, and contributes more robust deep face recognition models. Extensive experiments of models trained on CASIA-WebFace, VGGFace2, and MS-Celeb-1M databases, and evaluated on several face recognition benchmarks, such as LFW, MegaFace and IJB-C databases, have demonstrated the superiority of SFace.

2.
Crit Rev Food Sci Nutr ; : 1-22, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33435726

RESUMO

The antioxidant ability is the link and bridge connecting a variety of biological activities. Citrus flavonoids play an essential role in regulating oxidative stress and are an important source of daily intake of antioxidant supplements. Many studies have shown that citrus flavonoids promote health through antioxidation. In this review, the biosynthesis, composition and distribution of citrus flavonoids were concluded. The detection methods of antioxidant capacity of citrus flavonoids were divided into four categories: chemical, cellular, animal and clinical antioxidant capacity evaluation systems. The modeling methods, applicable scenarios, and their relative merits were compared based on these four systems. The antioxidant functions of citrus flavonoids under different evaluation systems were also discussed, especially the regulation of the Nrf2-antioxidases pathway. Some shortcomings in the current research were pointed out, and some suggestions for progress were put forward.

3.
J Agric Food Chem ; 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404218

RESUMO

Chemical structural characterization of phenolic compounds from the red ("Biqi") and white ("Shuijing") bayberries was carried out in the present study. With the aid of Global Natural Products Social Molecular Networking (GNPS), 18 flavonoid glycosides and 13 proanthocyanidins (PACs) in bayberry extracts were tentatively identified. Three cyanidin-3-glucoside derivatives (cyanidin-acetylapiosyl-glucoside, catechin-cyanidin-3-glucoside, and gallocatechin-cyanidin-3-glucoside), two quercetin derivatives (quercetin-3-arabinoside and quercetin-3-glucuronide), patuletin-7-glucoside, and individual PACs consisting of (epi)catechin or (epi)gallocatechin units were reported for the first time in bayberry fruits. In addition, "Biqi" exhibited a considerable increase of flavonoid glycoside content together with a dramatic decrease in the content of PACs in mature fruits, while "Shuijing" showed a decrease in levels of PACs but failed to accumulate flavonoid glycosides during fruit development.

4.
BMC Microbiol ; 21(1): 25, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33430787

RESUMO

BACKGROUND: Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. However, the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn't been characterized yet. RESULTS: Forty-three isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes, and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. The results of MLST were consist with the results of PFGE. CONCLUSIONS: These data showed that there might be a close homologous relationship between extraintestinal K. pneumoniae (EXKP) and enteral K. pneumoniae (EKP) in neonates, indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33387310

RESUMO

A novel eco-friendly molecularly imprinted polymer (MIP) was proposed as solid-phase extraction (SPE) adsorbent to selective adsorption tylosin (TYL) in animal muscle samples. The MIP was synthesized in aqueous by using 1,4-butanediyl-3,3-bis-1-vinyl imidazolium chloride and 2-acrylamide-2-methylpropanesulfonic acid as bifunctional monomer. The obtained MIP had excellent selectivity towards TYL in water, and the maximum binding capacity can reach 123.45 mg g-1. Combined with high-performance liquid chromatography, the presented MIP can be used as SPE sorbent to recognize and detect TYL in the range of 0.008 to 0.6 mg L-1 (R2 = 0.9995). The limit of detection and limit of quantification were 0.003 mg L-1 and 0.008 mg L-1, and the intraday and interday precision were 1.05% and 3.36%, respectively. Under the optimal condition, the established MIP-SPE-HPLC method was successfully applied to separate and determine trace TYL in chicken, pork, and beef samples with satisfactory recoveries ranged from 94.0 to 106.3%, and the MIP-SPE cartridge can be cycled at least 20 times. This study implies a promising green MIP-SPE-HPLC method for highly selective adsorption and analysis trace TYL in complex matrices.

6.
Ecotoxicol Environ Saf ; 208: 111617, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396137

RESUMO

Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3- levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose-dependent histopathological damage induced by NO3- exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3- significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRT-PCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3- exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3- level for juvenile turbot culture should not exceed 50 mg/L NO3-N in RAS. Our findings indicate that endocrine dysfunction of the GH/IGF-1, HPT, and HPI axes might be responsible for growth inhibition induced by NO3- exposure.

7.
Mol Med Rep ; 23(3)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33398374

RESUMO

Colorectal cancer (CRC) has a significant burden on healthcare systems worldwide, and is associated with high morbidity and mortality rates in patients. In 2020, the estimated new cases of colon cancer in the United States are 78,300 in men and 69,650 in women. Thus, developing effective and novel alternative agents and adjuvants with reduced side effects is important to reduce the lethality of the disease and improve the quality of life of patients. Melatonin, a pineal hormone that possesses numerous physiological functions, including anti­inflammatory and antitumor activities, can be found in various tissues, including the gastrointestinal tract. Melatonin exerts anticarcinogenic effects via various mechanisms; however, the identified underlying molecular mechanisms do not explain the full breadth of anti­CRC effects mediated by melatonin. MicroRNAs (miRs) serve critical roles in tumorigenesis, however, whether melatonin can inhibit CRC by regulating miRs is not completely understood. In the present study, the roles and mechanism underlying melatonin in CRC were investigated. The proliferation of human CRC cells was tested by CCK8, EDU and colony formation assay. The apoptosis of cancer cells was detected by flow cytometry and western blotting. A xenograft mouse model was constructed and the proliferation and apoptosis of tumor tissue was detected by Ki­67 and TUNEL staining assay respectively. Reverse transcription­quantitative PCR and western blotting were performed to measure the regulation of miRs on mRNA, and the dual­luciferase report analysis experiment was used to verify the direct target genes of miRs. Compared with the control group, melatonin inhibited viability and proliferation, and induced apoptosis in CRC cells. Additionally, the effect of melatonin in a xenograft mouse model was assessed. Compared with the control group, melatonin significantly enhanced the expression levels of the miR­34a/449a cluster, reduced CRC cell proliferation and viability, and increased CRC cell apoptosis. Finally, the dual­luciferase reporter assay indicated that Bcl­2 and Notch1 were the target mRNAs of the miR­34a/449a cluster. To the best of our knowledge, the present study was the first to suggest that melatonin inhibited proliferation and viability, and promoted apoptosis in CRC cells via upregulating the expression of the miR­34a/449a cluster in vitro and in vivo. Therefore, melatonin may serve as a potential therapeutic for CRC.

8.
Biomed Pharmacother ; 133: 111054, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33254022

RESUMO

Acute myeloid leukemia harboring internal tandem duplication of FMS-like tyrosine kinase 3 (FLT3-ITD AML) is a subset of highly aggressive malignancies with poor clinical outcome. Despite some advances in the development of FLT3 tyrosine kinase inhibitors (FLT3 inhibitors), most of FLT3-ITD AML patients suffer from lethal disease relapse, suggesting the requirement of novel targets and agents. Here we describe a natural small molecule, triptonide that can efficiently inhibit FLT3-ITD-driven AML in vitro and in vivo. Mechanistically, triptonide targeted Hedgehog/FLT3 signaling by inhibiting its critical effectors, which are GLI2, c-Myc and FLT3 and induced apoptosis of FLT3-ITD-driven leukemia cells. In addition, we also observed that triptonide activated tumor suppressor p53. In vivo, triptonide treatment markedly suppressed lethal FLT3-ITD-driven AML with good tolerance and prolonged survival time in orthotopic mouse model. Our studies identify Hedgehog/FLT3 axis as a novel target for treating FLT3-ITD-driven leukemia and demonstrate that triptonide is an active lead compound that can kill FLT3-ITD-driven leukemia cells.

9.
Bioresour Technol ; 319: 124216, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33254449

RESUMO

A novel gas-pressurized (GP) torrefaction with high oxygen removal efficiency at mild temperature was proposed in our previous work. However, the optimal condition of the GP torrefaction and subsequent pyrolysis of the torrefied biomass were not clear. In this work, the effect of pressure on the GP torrefaction and pyrolysis product properties of the torrefied biomass were studied in detail. The results show that the pressure increasing from 1.7 MPa to 5.0 MPa just slightly contributed to further oxygen removal, and 1.7 MPa was thus selected as the optimum pressure. The GP torrefaction significantly improved the product property of biomass pyrolysis compared to the conventional torrefaction (AP torrefaction). The acids content in bio-oil was reduced from 15-20% to less than 5%, and the calorific value of biogas increased to as high as 16.57-19.31 MJ/Nm3. Furthermore, an overall conversion mechanism of combined GP torrefaction and subsequent pyrolysis of biomass was proposed.


Assuntos
Biocombustíveis , Pirólise , Biomassa , Temperatura Alta , Pressão , Temperatura
10.
Int J Cardiol ; 323: 7-12, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32810549

RESUMO

BACKGROUND: We aimed to analyze the association of estimated glomerular filtration rate (eGFR) levels of hospitalized patients with treatment decisions and clinical outcomes in Chinese patients with acute coronary syndrome (ACS). METHODS: This was a secondary analysis study from CPACS-2 Program which was a trial of a quality improvement intervention in China and recruited 15,141 patients from 75 hospitals between October 2007 and August 2010. All patients were divided into three groups by the eGFR level on admission. The primary outcomes were several key performance indicators (KPIs) reflecting the management of ACS and the secondary outcomes were clinical outcomes. RESULTS: A total of 14,437 ACS patients were enrolled in this analysis. Among patients with reduced eGFR levels, fewer patients received appropriate medical therapy (p for trend <0.001) and fewer high-risk patients received coronary angiography (p for trend <0.001) compared to patients with a normal eGFR. Furthermore, 436 cases of death, 357 cases of cardiac death, 686 cases of major adverse cardiovascular events, and 198 cases of major bleeding episodes were reported. Patients with a worse eGFR level had significantly higher rates of death (p for trend <0.001), cardiac death (p for trend <0.001), major adverse cardiovascular events (p for trend <0.001) and major bleeding episodes (p for trend <0.001). CONCLUSION: Among Chinese ACS patients, those with renal insufficiency have a lower percentage of adherence to guideline-recommended treatments and worse clinical prognosis. Renal insufficiency is an important factor affecting guideline implementation in Chinese ACS patients. CLINICAL TRIAL REGISTRATION: http://www.anzctr.org.au/default.aspx. Unique identifier: ACTRN12609000491268.

11.
Ecotoxicol Environ Saf ; 207: 111287, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32931967

RESUMO

Coming along with high water reuse in sustainable and intensive recirculating aquaculture systems (RASs), the waste products of fish in rearing water is continuously accumulated. Nitrate, the final product of biological nitrification processes, which may cause aquatic toxicity to fish in different degrees when exposed for a long time. Therefore, the present study was conducted to evaluate the impact of chronic nitrate exposure on intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot. For that, groups of juvenile turbot were exposed to 0 (control check, CK), 50 (low nitrate, L), 200 (medium nitrate, M), and 400 (high nitrate, H) mg L-1 nitrate-N in small-sized recirculating aquaculture systems. After the 60-day experiment period, we found that exposure to a high concentration of nitrate-N caused obvious pathological damages to the intestine; for instance, atrophy of intestinal microvilli and necrosis in the lamina propria. Quantitative real-time PCR analysis revealed a significant downregulation of the barrier forming tight junction genes like occludin, claudin-like etc. under H treatment (P < 0.05). Intestinal MUC-2 expression also decreased significantly in the nitrate treatment groups compared to that in the control (P < 0.05). Additionally, the expression of HSP70 and HSP90 heat-shock proteins, toll-like receptor-3 (TLR-3), interleukin-1ß (IL-1ß), and tumor necrosis factor-α (TNF-α) significantly increased (P < 0.05), whereas that of transforming growth factor-ß (TGF-ß), lysozyme (LYS), and insulin-like growth factor-I (IGF-I) significantly decreased with H treatment (P < 0.05). The results also revealed that intestinal microbial community was changed following nitrate exposure and could alter the α-diversity and ß-diversity. Specifically, the proportion of intrinsic flora decreased, whereas that of the potential pathogens significantly increased with M and H treatments (P < 0.05). In conclusion, chronic nitrate exposure could weaken the barrier function and disturb the composition of intestinal microbiota in marine teleosts, thereby harming their health condition.


Assuntos
Linguados/crescimento & desenvolvimento , Microbioma Gastrointestinal/efeitos dos fármacos , Imunidade nas Mucosas/efeitos dos fármacos , Mucosa Intestinal/efeitos dos fármacos , Intestinos/efeitos dos fármacos , Nitratos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , DNA Bacteriano/genética , Relação Dose-Resposta a Droga , Linguados/microbiologia , Mucosa Intestinal/imunologia , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Intestinos/microbiologia
12.
J Cell Mol Med ; 25(1): 132-146, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33191645

RESUMO

It has been reported that chemokine CX3 CL1 can regulate various tumours by binding to its unique receptor CX3 CR1. However, the effect of CX3 CL1-CX3 CR1 on the lung adenocarcinoma and lung squamous cell carcinoma is still unclear. Here, we showed that CX3 CL1 can further invasion and migration of lung adenocarcinoma A549 and lung squamous cell carcinoma H520. In addition, Western blot and immunofluorescence test indicated CX3 CL1 up-regulated the phosphorylation level of cortactin, which is a marker of cell pseudopodium. Meanwhile, the phosphorylation levels of c-Src and c-Abl, which are closely related to the regulation of cortactin phosphorylation, are elevated. Nevertheless, the src/abl inhibitor bosutinib and mutations of cortactin phosphorylation site could inhibit the promotion effect of CX3 CL1 on invasion and migration of A549 and H520. Moreover, these results of MTT, Hoechst staining and Western blot suggested that CX3 CL1 had no effect on the proliferation and apoptosis of A549 and H520 in vitro. The effects of CX3 CL1 were also verified by the subcutaneous tumour formation in nude mice, which showed that it could promote proliferation and invasion of A549 in vivo. In summary, our results indicated that CX3 CL1 furthered invasion and migration in lung cancer cells partly via activating cortactin, and CX3 CL1 may be a potential molecule in regulating the migration and invasion of lung cancer.

13.
Cell Mol Immunol ; 18(1): 230-242, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32203192

RESUMO

The exact relationships between group 2 innate lymphoid cells (ILC2s) and Th2 cells in type 2 pathology, as well as the mechanisms that restrain the responses of these cells, remain poorly defined. Here we examined the roles of ILC2s and Th2 cells in type 2 lung pathology in vivo using germline and conditional Relb-deficient mice. We found that mice with germline deletion of Relb (Relb-/-) spontaneously developed prominent type 2 pathology in the lung, which contrasted sharply with mice with T-cell-specific Relb deletion (Relbf/fCd4-Cre), which were healthy with no observed autoimmune pathology. We also found that in contrast to wild-type B6 mice, Relb-deficient mice showed markedly expanded ILC2s but not ILC1s or ILC3s. Moreover, adoptive transfer of naive CD4+ T cells into Rag1-/-Relb-/- hosts induced prominent type 2 lung pathology, which was inhibited by depletion of ILC2s. Mechanistically, we showed that Relb deletion led to enhanced expression of Bcl11b, a key transcription factor for ILC2s. We concluded that RelB plays a critical role in restraining ILC2s, primarily by suppressing Bcl11b activity, and consequently inhibits type 2 lung pathology in vivo.

14.
Bioresour Technol ; 321: 124502, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33310409

RESUMO

In this study, torrefaction behavior of hot-pressed wood pellet prepared at 250 °C and compression pressure of 70 MPa was examined at temperature 260-300 °C. It was found that the torrefaction behavior of hot-pressed pellet (HP) was significantly different from that of Raw and cold-pressed pellet (CP). The mass yield and energy yield for torrefaction at 300 °C and 30 min holding time for HP were 54.5% and 84.4%, respectively. Whereas the mass yield and energy yield for torrefaction at 300 °C and 30 min for Raw were 41.5% and 58.1%, respectively. From the gas formation analysis, it was found that the dehydration and deoxygenation reactions were accelerated to produce a large amount of H2O and CO2 during the torrefaction of HP. It was judged that torrefaction of hot-pressed pellet was very effective to prepare high quality black pellet.


Assuntos
Madeira , Biomassa , Pressão , Temperatura
15.
Bioresour Technol ; 321: 124505, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33316697

RESUMO

Co-gasification of coal and biomass offers a relatively cleaner utilization way of fossil fuel. The fuel property improvement of biomass can not only improve the property of syngas but also enhance the synergistic effect during the co-gasification. In our previous work, a novel gas-pressurized (abbreviated as GP) torrefaction was proposed to effectively upgrade the biomass under mild condition. In this work, the co-gasification of GP torrefied biomass and coal were conducted to explore the synergistic effect and kinetics. Significant synergistic effect during the co-gasification was proved. The CO yield of co-gasification increased to as high as 70.70 mol/kg, resulting from the promotion of carbon in coal converting into CO by GPRS. Furthermore, the kinetic model of RPM was most fitting for the co-gasification, and the activation energy of co-gasification was reduced. Thus, the coal gasification was promoted significantly by GP torrefied biomass through obvious synergistic effect during the co-gasification.


Assuntos
Carbono , Carvão Mineral , Biomassa , Cinética
16.
J Pharm Biomed Anal ; 194: 113816, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33339640

RESUMO

Mass spectrometry based precision identification of natural products requires the validation of the reference compounds. This study attempted to develop a LC-QTOF MS combined with LC-TQ MS method to precisely characterize the chemicals of Si-Ni-San (SNS), a classic traditional Chinese medicine formula, which is composed of four medicinal plants, and widely used for the treatments of liver disorders. 74 compounds in SNS were provisionally identified by acquiring MS spectra and MS/MS spectra of the possible chemical features, as well as retrieving small-molecule database. By comparing with the accurate MS/MS spectra of reference compounds, 37 compounds in SNS were precisely identified for the first time. In addition, our effort also successfully assigned the origin of each identified compounds against four medicinal plants. Furthermore, we developed a UHPLC-TQ MS based quantitative-profiling method for simultaneous determination of 37 targeted compounds in the different extracts of the raw SNS and commercial lyophilized powders, enabling to facilitate overall quality control of SNS and associated commercial products. Collectively, our finding precisely characterized the main chemicals in SNS, which also provides a new strategy with LC-MS/MS based chemical profiling to precisely identify a diversity of chemicals in Chinese medicinal plants and associated formulae.

17.
Huan Jing Ke Xue ; 42(1): 274-282, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372479

RESUMO

In order to study the performance and mechanisms of bioretention pond media (Enteromorpha prolifera biochar) for NH4+-N removal in rainfall runoff, three kinds of alkali modified biochars (marked as BC1, BC2, and BC3) were prepared with various concentrations of NaOH solution (1, 2, and 3 mol·L-1) to explore their adsorption performance for NH4+-N. The results showed that:① Appropriate modifications of the NaOH concentration increased the specific surface area and surface microstructure of biochar, with the content of O and the surface functional groups being enriched. In addition, BC2 possessed the best adsorption performance. ② The adsorption capacity reached a maximum when the pH was 9.0 and the dosage of biochar was 0.5 g·L-1. Compared with BC, the adsorption capacity of BC1 and BC2 increased by 6.4% and 10.8%, respectively, while BC3 decreased by 13.7%. Moreover, BC2 had an optimal adsorption efficiency with a saturated adsorption capacity of 16.76mg·g-1. ③ The adsorption mechanism of biochar belonged to chemical adsorption with a monomolecular layer. The adsorption process was promoted by the high pH of biochar, the electrostatic attraction of biochar pores, the complexation and oxidization of the functional groups of hydroxyl (-OH), carboxyl (-COOH), and carbon-oxygen single bond (C-O). To sum up, the proper amount of NaOH to modify biochar can improve the adsorption performance of NH4+-N, and the modified biochar can be used as media of the bioretention pond to remove NH4+-N.


Assuntos
Amônia , Carvão Vegetal , Adsorção , Nitrogênio
18.
Clin Interv Aging ; 15: 2277-2289, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33304098

RESUMO

Contrast-induced encephalopathy (CIE) is a rare complication following percutaneous carotid and coronary interventions, and important diagnostic radiological signs include brain edema and cortical enhancement. In this report, we detail a case of probable CIE in an 84-year-old woman following a normal diagnostic coronary angiography (CAG) that involved 20 mL of the low-osmolar, non-ionic monomeric, iodine-based contrast agent iopromide (Ultravist 370). The patient was unconscious and presented with hemiparesis, hemianopia, recurrent seizures, and cardiac and respiratory arrest within minutes to hours following the procedure. Non-contrast computed tomography (CT) of the head showed increased subarachnoid density, cortical enhancement, and brain edema in the right hemisphere. Three days of rehydration, reduction in cranial pressure, and treatment with an anticonvulsant and dexamethasone resulted in a gradual recovery with no neurological deficits. This case highlights that severe neurotoxic symptoms may occur in response to low doses of low-osmolar, non-ionic, monomeric contrast agents. This finding is of importance to interventional cardiologists for diagnostic considerations and development of treatment plans.

19.
Am J Med Sci ; 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33268054

RESUMO

BACKGROUND: Previous studies have shown an inverse relationship between education and clinical outcomes in coronary heart disease. Whether a similar association exists in patients presenting with suspected acute coronary syndromes (ACS) in China is unknown. METHODS: Clinical Pathways for Acute Coronary Syndromes - Phase 2 (CPACS-2) was a study to evaluate a quality improvement for ACS management in China which implemented in 75 hospitals between October 2007 and August 2010. All patients was divided into 6 groups by education level. We evaluated clinical managements and outcomes according to level of education. RESULTS: A total of 14350 patients were enrolled in current analysis. Patients with less education were older, had greater female representation and had a higher Killip class at admission. Compared to patients with lower education levels, more educated patients had a longer length of hospitalization (p for trend <0.001), greater likelihood of receiving appropriate coronary angiography (p for trend <0.001) and appropriate reperfusion therapy for ST-segment elevated myocardial infarction (STEMI) (p for trend <0.001) even after adjustment for differences in patient characteristics and comorbidities at presentation. Patients with less education were at higher risk of death, cardiac death and major adverse cardiovascular events but none of these differences remained statistically significant after adjustment for baseline characteristics. CONCLUSION: In China, less educated patients with ACS were less likely to receive appropriate coronary angiography and reperfusion therapy. Less educated patients were at higher risk for adverse clinical events; however this was explained by differences in baseline characteristics.

20.
Mediators Inflamm ; 2020: 3824593, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33343230

RESUMO

Tantalum (Ta) is gaining attention as a biomaterial in bone tissue engineering. Although the clinical advantage of Ta-based implants for primary and revision total joint replacement (TJA) has been well documented, few studies investigated the effect of wear products of Ta implants on peri-implant cells, and their potential contribution to aseptic implant loosening. This study is aimed at examining the cytotoxicity, oxidative stress, and proinflammatory potential of Ta and TiO2 nanoparticles (NPs) on macrophages in vitro. NPs were characterized using scanning electron microscopy, dynamic light scattering, and energy-dispersive X-ray. To test the NP-mediated cellular response in macrophages, THP-1-derived macrophages were challenged with both NPs, and cytotoxicity was analyzed using CCK-8 and LDH assays. Flow cytometry was used to investigate particle uptake and their internalization routes. NP-mediated oxidative stress was investigated by measuring the production of reactive oxygen species, and their proinflammatory potential was determined by quantifying the production of TNFα and IL-1ß in cell culture supernatants using ELISA. We found that both Ta and TiO2 NPs were taken up through actin-dependent phagocytosis, although TiO2 NPs did also show some involvement of macropinocytosis and clathrin-mediated endocytosis. Ta NPs caused no apparent toxicity, while TiO2 NPs demonstrated significant cytotoxicity at a concentration of over 100µg/mL at 24 h. Ta NPs induced negligible ROS generation and proinflammatory cytokines (TNFα, IL-1ß) in macrophages. In contrast, TiO2 NPs markedly induced these effects in a dose-dependent manner. Our findings indicate that Ta NPs are inert, nontoxic, and noninflammatory. Therefore, Ta could be considered an excellent biomaterial in primary and revision joint arthroplasty implants.

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