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1.
J Phys Chem Lett ; : 4193-4197, 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-32370503

RESUMO

Exploring 2D multifunctional materials with intrinsic ferro-/ferrimagnetism and vertical ferroelectricity is a highly desirable but challenging task. Here, motivated by the recently synthesized organometallic frameworks K3Fe2[PcFeO8], we propose to realize such materials in a series of 2D K3M2[PcMO8] (M = Cr-Co) nanosheets. First-principles calculations suggest 2D K3Cr2[PcCrO8] as a ferromagnetic half metal with a Curie temperature of 140 K, whereas others (M = Mn, Fe, and Co) are all ferrimagnetic semiconductors with the Curie temperatures between 66 and 150 K. Moreover, the structural distortion due to the out-of-plane K+ counterions leads to a significant vertical electric polarization. The estimated intensity of polarization for K3Fe2[PcFeO8] is 143 pC/m, with the ferroelectric phase-transition barrier being 0.38 eV per formula. This work highlights the potential of 2D organometallic frameworks such as K3M2[PcMO8] as a versatile platform for designing multifunctional materials with simultaneous ferro-/ferrimagnetism and vertical ferroelectricity.

3.
J Biomed Inform ; 107: 103422, 2020 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-32353595

RESUMO

Clinical Named Entity Recognition (CNER) is a critical task which aims to identify and classify clinical terms in electronic medical records. In recent years, deep neural networks have achieved significant success in CNER. However, these methods require high-quality and large-scale labeled clinical data, which is challenging and expensive to obtain, especially data on Chinese clinical records. To tackle the Chinese CNER task, we pre-train BERT model on the unlabeled Chinese clinical records, which can leverage the unlabeled domain-specific knowledge. Different layers such as Long Short-Term Memory (LSTM) and Conditional Random Field (CRF) are used to extract the text features and decode the predicted tags respectively. In addition, we propose a new strategy to incorporate dictionary features into the model. Radical features of Chinese characters are used to improve the model performance as well. To the best of our knowledge, our ensemble model outperforms the state of the art models which achieves 89.56% strict F1 score on the CCKS-2018 dataset and 91.60% F1 score on CCKS-2017 dataset.

5.
Biomaterials ; 248: 119981, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32276041

RESUMO

Regarding the high requirement of cardiac and vascular implants in tissue engineering, a novel concept of surface chemistry strategy featuring multiple functions is proposed in this study, which provides glutathione peroxidase (GPx)-like catalytic activity and allows secondary reactions for grafting functional biomolecules. The suggested strategy is the fabrication of a metal-catechol-(amine) network (MCAN) containing copper ions with GPx-like activity, amine-bearing hexamethylenediamine (HD) and wet adhesive catechol dopamine (DA). With a simple one-step molecular/ion co-assembly, the developed copper-DA-HD (CuII-DA/HD) network can be used to catalyze the generation of therapeutic nitric oxide (NO) gas in a durable and dose-controllable manner. The primary amine groups in the CuII-DA/HD network facilitate the secondary immobilization of bivalirudin (BVLD) to further provide an antithrombotic activity as supplement to the functions of NO. The CuII-DA/HD + BVLD coating functionalized on cardiovascular stents successfully improved thromboresistance, anti-restenosis, and promotes re-endothelialization in vivo. With regard to the ease of operation and low cost, the synergetic modification using MCAN strategy is of great potential for developing multifunctional blood-contacting materials/devices.

6.
Sci Total Environ ; 727: 138790, 2020 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-32344260

RESUMO

Although the associations between endosulfan and adverse cardiovascular health have been reported, the toxic effects and underlying mechanism of endosulfan on the heart are not well understood. In this study, we examined the cardiotoxicity induced by endosulfan using Wistar rats and human cardiomyocytes (AC16) cells. Wistar rats were divided into control group (received corn oil alone) and three concentrations of endosulfan groups (1, 5 and 10 mg/kg·bw) by gavage. The AC16 cells were treated with three various concentrations (0, 1.25, 5, and 20 µg/mL) of endosulfan. The results showed that endosulfan induced cytotoxicity through damaging myocardial structure, decreasing the viability of cardiomyocytes, and elevating the serum levels of cardiac troponin I, heart fatty acid binding protein, aspartate aminotransferase, and reactive oxygen species (p < 0.05). Moreover, measurement of mitochondrial function showed that endosulfan could significantly decrease adenosine triphosphate levels and cytochrome c oxidase IV expression in AC16 cells (p < 0.05). In addition, endosulfan obviously inhibited Bcl-2 expression, activated the expressions of cytochrome c/Caspase-9/Caspase-3 signaling pathway, and induced the apoptosis of AC16 cells (p < 0.05). Furthermore, endosulfan significantly increased the expression of Bim, and inhibited the expressions of PI3K/Akt/FoxO3a signaling pathways in cardiomyocytes (p < 0.05). These results suggest that endosulfan may induce cardiotoxicity by inducing myocardial apoptosis resulting from activation of mitochondria-mediated apoptosis pathway and inhibition of pro-survival signaling pathways, which might be helpful in elucidating the mechanism of cardiac dysfunction induced by endosulfan.

7.
Molecules ; 25(6)2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32178315

RESUMO

Walnut residue is a kind of high-quality plant protein resource. The bioactive peptide prepared from walnut residue has excellent health care functions such as antioxidation and antihypertensive activity, but at present, walnut residue is often regarded as waste or low value feed, fertilizer and other materials. The uneconomical use of walnut residue has hindered the development of the walnut industry to some extent. Effective utilization of walnut residue protein to develop bioactive peptides and other products is of great significance to realize the comprehensive utilization of walnut residue, improve the added value of by-products, and change the current low utilization rate of walnut residue. In this paper, the preparation, purification and structure identification of walnut protein bioactive peptides are reviewed, and different functional walnut active peptides (WBPs) are introduced. The potential effects of these bioactivities on human health and their different uses in food, medicine and other industries are discussed. The purpose is to provide reference information for the effective utilization of walnut residue resources and the development of walnut industry.

8.
Bioinformatics ; 2020 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-32221617

RESUMO

MOTIVATION: Comparing the organization of gene, gene clusters, and their flanking genomic contexts is of critical importance to the determination of gene function and evolutionary basis of microbial traits. Currently, user-friendly and flexible tools enabling to visualize and compare genomic contexts for numerous genomes are still missing. RESULTS: We here present Gcluster, a stand-alone Perl tool that allows researchers to customize and create high-quality linear maps of the genomic region around the genes of interest across large numbers of completed and draft genomes. Importantly, Gcluster integrates homologous gene analysis, in the form of a built-in orthoMCL, and mapping genomes onto a given phylogeny to provide superior comparison of gene contexts. AVAILABILITY AND IMPLEMENTATION: Gclusteris written in Perl and released under GPLv3. The source code is freely available at https://github.com/Xiangyang1984/Gcluster. Gcluster can also be installed through conda: "conda install -c bioconda gcluster". SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

9.
Inflammation ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32170601

RESUMO

Trained immunity has been recently identified in innate immune cells, which undergo long-term epigenetic and metabolic reprogramming after exposure to pathogens for protection from secondary infections. (1, 3)/(1, 6)-ß-glucan derived from fungi can induce potent trained immunity; however, the effect of (1, 3)/(1, 4)-ß-glucan (rich in dietary fiber oat) on trained immunity has not been reported. In the present study, two cell culture systems for trained immunity induction were validated in monocytes/macrophages from mouse bone myeloid and human THP-1 cells exposed to positive inducers of trained immunity, including ß-glucan from Trametes versicolor or human-oxidized low-density lipoprotein. Primed with oat ß-glucan, the mRNA expression and production of TNF-α and IL-6 significantly increased in response to re-stimulation of TLR-4/2 ligands. Moreover, the expression of several key enzymes in glycolytic pathway and tricarboxylic acid cycle was significantly upregulated. In addition, inhibiting these enzymes decreased the production of TNF-α and IL-6 boosted by oat ß-glucan. These results show that oat ß-glucan induces trained immunity through metabolic reprogramming. This provides important evidence that dietary fiber can maintain the long-term responsiveness of the innate immune system, which may benefit for prevention of infectious diseases or cancers.

10.
BMC Immunol ; 21(1): 7, 2020 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-32066378

RESUMO

BACKGROUND: Clonorchis sinensis infection could trigger strong immune responses in mice and humans. However, whether the C.sinensis infection has an impact on arthritis is unknown. Here we investigated the effect of C.sinensis infection on type II collagen-induced arthritis in BALB/c mice. RESULTS: The mice were firstly infected with 45 C.sinensis metacercariae by oral gavage. Four weeks later, arthritis in mice was induced by type II collagen. Joint inflammation with severe redness and swelling in hind paws was observed in type II collagen-induced arthritis (CIA) mice. Besides, the physical activity was significantly reduced, but the respiratory exchange ratio was increased in CIA mice. Compared with CIA mice, C.sinensis infection could increase the severity of arthritis in CIA mice, based on the results of disease score and pathological changes. Compared to CIA mice, increased neutrophils and Ly6Chi monocytes, decreased B cells and CD4+T cells, were found in C.sinensis infected CIA mice. Besides these, C.sinensis infected mice also displayed significantly higher levels of serum IL-4 and IL-17 than those in CIA mice. CONCLUSIONS: Taken together, our data suggest that C.sinensis infection have a bad effect on arthritis, and could induce the abnormality of the immune response in mice with CIA.

11.
Artigo em Inglês | MEDLINE | ID: mdl-32061879

RESUMO

OBJECTIVES: Older antimicrobials, such as fosfomycin, are being considered as alternative agents in the treatment of drug-resistant organisms; however, there is limited data on the usefulness of fosfomycin in carbapenem-resistant Klebsiella pneumoniae (CRKP). The aim of this study was to investigate the prevalence of fosfomycin resistance and associated mechanisms in CRKP. METHODS: 99 CRKP clinical strains were collected in the Second Affiliated Hospital of Wenzhou Medical University (Wenzhou, China) between January 2017 and June 2018. Fosfomycin susceptibility testing was carried out using the agar dilution method. Carbapenemases and fosfomycinases genes were detected by PCR, Analysis of themurA, glpT, uhpT, uhpA, pstI and cyaA genes was performed by PCR and sequencing four fosfomycin-resistant fosA3-negative CRKP strains. Conjugation experiments were employed to determine the mobility of fosA3 gene. RESULTS: Fosfomycin non-susceptibility was detected in 48.5% (48/99) isolates, and thefosA3 gene was detected in 44 strains of fosfomycin non-susceptible CRKP. Among the four fosfomycin-resistant fosA3-negative CRKPs, one strain possessed single nucleotide insertion and deletion mutations, 219 nucleotide substitution mutations in murA; two strains possessed deletion or mutation of large DNA fragments in glpT; one strains possessed a fragment insertion sequence in glpT. Transfection into Escherichia coli J53 via plasmid conjugation, was successful for 19 of the 44 fosA3-positive CRKPs (43.2%). CONCLUSIONS: ThefosA3 gene is the primary mechanism of fosfomycin resistance in CRKP and can be transmitted widely by plasmid in hospitals. Mutations in murA and glpT were found in fosfomycin-resistant fosA3-negative CRKP.

12.
Biomaterials ; 241: 119904, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32109705

RESUMO

Specific selectivity of vascular cells and antithrombogenicity are crucial factors for the long-term success of vascular implants. In this work, a novel concept of mussel-inspired "built-up" surface chemistry realized by sequential stacking of a copper-dopamine network basement, followed by a polydopamine layer is introduced to facilitate the combination of nitric oxide (NO) catalysis and vascular cell selectivity. The resultant "built-up" film allowed easy manipulation of the content of copper ions and the density of catechol/quinone groups, facilitating the multifunctional surface engineering of vascular devices. For example, the chelated copper ions in the copper-dopamine network endow a functionalized vascular stent with a durable release of NO via catalytic decomposition of endogenous S-nitrosothiol. Meanwhile, the catechol/quinone groups on the film surface allow the facile, secondary grafting of the REDV peptide to develop a selectivity for vascular cells, as a supplement to the functions of NO. As a result, the functionalized vascular stent perfectly combines the functions of NO and REDV, showing excellent antithrombotic properties and competitive selectivity toward the endothelial cells over the smooth muscle cells, hence impressively promotes re-endothelialization and improves anti-restenosis in vivo. Therefore, the first mussel-inspired "built-up" surface chemistry can be a promising candidate for the engineering of multifunctional surfaces.

13.
Commun Biol ; 3(1): 62, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32047240

RESUMO

African swine fever virus (ASFV), the aetiological agent of African swine fever (ASF), causes lethal haemorrhagic fever in domestic pigs with high mortality and morbidity and has devastating consequences on the global swine industry. On-site rapid and sensitive detection of ASFV is key to the timely implementation of control. In this study, we developed a rapid, sensitive and instrument-free ASFV detection method based on CRISPR/Cas12a technology and lateral flow detection (named CRISPR/Cas12a-LFD). The limit of detection of CRISPR/Cas12a-LFD is 20 copies of ASFV genomic DNA per reaction, and the detection process can be completed in an hour. The assay showed no cross-reactivity with other swine DNA viruses, and has 100% agreement with real-time PCR detection of ASFV in 149 clinical samples. Overall, the CRISPR/Cas12a-LFD method provides a novel alternative for the portable, simple, sensitive, and specific detection of ASFV and may contribute to the prevention and control of ASF outbreaks.

14.
Food Chem ; 316: 126303, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32045813

RESUMO

As the accumulation of mercury ions has a detrimental impact on human health, the design and development of a new type of biosensor that can rapidly, sensitively and selectively detect Hg2+ in aqueous solutions are essential. In this study, we have developed an exonuclease III (ExoIII) and Terminal deoxynucleotidyl transferase (TdT) dependent isothermal amplification (ETDA) colorimetric biosensor. The template sequence is a hairpin where -NH2 is labeled at the 3'-end and both termini are T-rich sequences. In the presence of Hg2+, the template formed a blunt end, and the catalytic activity of ExoIII was activated with cleavage of the -NH2 at the 3'-end. TdT enzyme activity was initiated with the formation of a large number of G-rich nucleic acid sequences. G-rich sequences incubated with iron (III)-hemin mimicked peroxidase-like activity, catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. The biosensor constructed in this paper had a good linear range, 1-25 nmol/L. Its detection limit was 0.41 nmol/L (3σ), and recovery rates were between 100.5% and 103%. In conclusion, combined with the colorimetric biosensor and double enzyme cyclic amplification reaction, an ultra-sensitivity and strong specificity detection method was developed to detect Hg2+. At the same time, this method also expands the detection method of Hg2+ available in the literature.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31898151

RESUMO

The effects of the short-term application of Ascophyllum nodosum-fermented seaweed fertilizer on the bacterial community, soil nitrogen contents, and plant growth in maize rhizosphere soil were evaluated. The changes in the bacterial community composition and nitrogen contents including those of total nitrogen (TN), nitrate nitrogen (NO3--N) and ammonium nitrogen (NH4+-N) in rhizosphere soils in response to treatment with seaweed fertilizer were determined. Furthermore, soil enzymatic activity and crop biomass were analyzed. The relative abundance of the dominant phyla varied regularly with fertilization, and bacterial α-diversity was apparently influenced by seaweed fertilizer amendment. The TN contents of all soil samples decreased gradually, and the NO3--N and NH4+-N contents of the soils treated with seaweed fertilizer were much higher than those of the control soils. Similarly, the enzymatic activities of dehydrogenase, nitrite reductase, urease, and cellulase in the soil were significantly increased on day 3, day 8, and day 13 after the application of seaweed fertilizer to the maize rhizosphere soil. However, there was no difference in the activity of soil sucrase between the treatment group and the control group. In this study, the growth of maize seedlings was confirmed to be greatly promoted by the utilization of seaweed fertilizer. These results deepen our understanding of plant-microbe interactions in agroecosystems and should benefit the wide use of seaweed fertilizer in sustainable agricultural production.

16.
Inflammation ; 43(2): 731-743, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31907686

RESUMO

Disordered glucose and lipid metabolism contributes to the progression of several liver diseases, while the upregulation of phosphatase and tensin homology deleted on chromosome ten (PTEN), a well-known tumour suppressor gene, can improve the condition through metabolic programming. This study first characterized the metabolic profiles and the involvement of PTEN in the hepatic fibrosis induced by Schistosoma japonicum (S. japonicum) to provide a novel clue for metabolism-targeted treatment. Compared with control mice, infected mice showed infiltrated immune cells in their livers, increased levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and decreased glucose levels in their sera. The expression of key enzymes in the glycolytic pathway was significantly increased, and the expression of gluconeogenic genes was distinctly decreased. Moreover, the infection upregulated the hepatic expression of enzymes involved in fatty acid oxidation, which was consistent with the decreased number of lipid droplets in livers and the lowered levels of triglyceride in sera. Consistently, PTEN and its downstream signalling were significantly inhibited. In vitro, soluble egg antigen (SEA) downregulated the expression of PTEN in both the macrophage RAW264.7 cell line and the murine hepatocellular carcinoma HEP1-6 cell line, and induced a metabolic phenotype similar to the in vivo results. Overall, this study showed that S. japonicum infection induced the reprogramming of glucose and lipid metabolism in mice during the period of liver fibrosis and that SEA could act as a modulator to trigger such a metabolic switch in macrophages and hepatocytes. PTEN might play an essential role in mediating these metabolic reprogramming events.

17.
Int J Biol Macromol ; 142: 376-383, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31593726

RESUMO

Corn/octenylsuccinated starch (C/OS) composite films incorporated with soybean oil (SO) at 0, 0.5%, 1.0%, 1.5% and 2.0% (w/w) were prepared to investigate their physicochemical properties. Fourier transform infrared analysis indicated that low concentrations of SO could facilitate molecular interaction and the formation of hydrogen bonds between starch molecules. All the films exhibited similar diffractograms and lower relative crystallinity values. Scanning electron microscopy and atomicforcemicroscopy showed that the irregular and coarse surface structures of the films were obtained more frequently with increasing SO concentration. A higher contact angle of 76.14° and lower water vapor permeability of 2.46 × 10-12 g cm/cm2 s Pa were obtained with increasing SO content, with the exception of the 2.0% SO sample. The highest tensile strength value of 6.54 MPa was obtained by the C/OS-1.0% SO composite film, while the optimumelongation at break of 71.84% was exhibited by the C/OS-1.5% SO composite film.

18.
Angew Chem Int Ed Engl ; 59(1): 203-208, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31663271

RESUMO

Two-dimensional (2D) hybrid perovskites have shown many attractive properties associated with their soft lattices and multiple quantum well structure. Herein, we report the synthesis and characterization of two new multifunctional 2D hybrid perovskites, (PED)CuCl4 and (BED)2 CuCl6 , which show reversible thermochromic behavior, dramatic temperature-dependent conductivity change, and strong ferromagnetism. Upon temperature change, the (PED)CuCl4 and (BED)2 CuCl6 crystals exhibit a reversible color change between yellow and red-brown. The associated structural changes were monitored by in situ temperature-dependent powder X-ray diffraction (PXRD). The (BED)2 CuCl6 exhibits superior thermal stability, with a thermochromic working temperature up to 443 K. The conductivity of (BED)2 CuCl6 changes over six orders of magnitude upon temperature change. The 2D perovskites exhibit ferromagnetic properties with Curie temperatures around 13 K.

19.
Polymers (Basel) ; 11(12)2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31810159

RESUMO

Isotactic polybutylene-1 (iPB) has lots of advantages and is best used as hot water pipe. However, to transform into stable crystal form I, the iPB needs as long as 7 days. In this process, the irreversible damage brings great difficulties to the use of the iPB. The method which convert it directly into crystal I has shortcomings such as being requiring complex operation and being expensive. In this study, an innovative idea was put forward, not paying attention to the crystal transformation of iPB but only focusing on reducing the time it can be applied. In this study, bamboo powder was modified by the silane coupling agent KH570 (KBP) to prepare iPB/KBP composite. The infiltration test and Fourier transform infrared (FTIR) analysis showed that the hydrophilicity of KBP is greatly reduced, which can greatly improve the compatibility of the iPB and KBP. The tensile strength, tensile modulus, flexural strength, and flexural modulus of the composites storage for 3 days is equal to the pure iPB with storage 7 days with the KBP additions of 3%, 3%, 7%, and 5%, respectively. The heat deformation temperature (HDT) of the composite with 3% KBP after 1-day storage reached the value of pure iPB storage for 7 days. This provides more space and possibilities for the industrialization of the iPB. The crystallization behavior of iPB/KBP composites proves that the addition of KBP accelerates the crystallization rate of iPB, but the crystallinity of the iPB/KBP composites is not changed. The SEM photograph of iPB/KBP composites showed that when the KBP addition was low the compatibility between KBP and iPB was good. When the KBP addition was increased the agglomeration of KBP in the iPB was very obvious, which leads to the poor mechanical properties of the composite.

20.
World J Gastroenterol ; 25(46): 6781-6789, 2019 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-31857779

RESUMO

BACKGROUND: A one-stage laparoscopic operation has recently been considered a favorable option for the management of patients with Hirschsprung's disease (HD) due to its superior cosmetic results. One-stage transanal endorectal pull-through for the treatment of rectosigmoid HD has been widely used in newborns without complications. However, enterostomy is required in some HD cases for enterocolitis and dilated colon. Our transumbilical enterostomy (TUE) and two-stage laparoscopy-assisted anorectoplasty were effective and achieved a similar cosmetic effect to one-stage laparoscopy on the abdominal wall in patients with anorectal malformation, but the effect in patients with HD is unclear. AIM: To evaluate the safety, efficacy and cosmetic results of TUE in two-stage laparoscopy-assisted pull-through for HD. METHODS: From June 2013 to June 2018, 53 patients (40 boys, 13 girls; mean age at enterostomy: 5.5 ± 2.2 mo) who underwent enterostomy and two-stage laparoscopy-assisted pull-through for HD with stoma closure were reviewed at our institution. Two enterostomy approaches were used: TUE in 24 patients, and conventional abdominal enterostomy (CAE) in 29 patients. Eleven patients with rectosigmoid HD had severe preoperative enterocolitis or a dilated colon. 26 patients had long-segment HD, and 16 patients had total colonic aganglionosis (TCA). The patients with left-sided HD underwent the two-stage laparoscopic Soave procedure, and the patients with right-sided HD and TCA underwent the laparoscopic Duhamel procedure. Demographics, enterostomy operative time, complications and cosmetic results were respectively evaluated. RESULTS: There were no differences between the groups with respect to gender, age at enterostomy, weight and clinical type (P > 0.05). No conversion to open technique was required. Two patients experienced episodes of stomal mucosal prolapse in the TUE group and 1 patient in the CAE group (8.33% vs 3.45%, P > 0.05). No parastomal hernia was observed in either of the two groups. Wound infection at the stoma was seen in 1 case in the TUE group, and 2 cases in the CAE group (4.17% vs 6.90%, P > 0.05). No obstruction was noted in any of the patients in the TUE group, whereas obstruction was found in 1 patient in the CAE group. Enterocolitis was observed in 3 and 5 patients in the TUE and CAE group, respectively (12.50% vs 17.24%, P > 0.05). There was no significant difference between the TUE group and CAE group in terms of the incidence of soiling and constipation (P > 0.05). The cosmetic result using the scar score in the TUE group was better than that in the CAE group (6.83 ± 0.96 vs 13.32 ± 1.57, P < 0.05). CONCLUSION: TUE is a safe and feasible method for the treatment of HD, and the staged enterostomy and two-stage laparoscopy-assisted pull-through achieved a similar cosmetic effect to the one-stage laparoscopic procedure.


Assuntos
Enterostomia/métodos , Doença de Hirschsprung/cirurgia , China/epidemiologia , Enterostomia/efeitos adversos , Enterostomia/estatística & dados numéricos , Feminino , Humanos , Lactente , Laparoscopia , Masculino , Cirurgia Endoscópica por Orifício Natural , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos
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