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1.
Anal Chim Acta ; 1071: 17-24, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31128751

RESUMO

In this study, novel porous carbon composites were successfully prepared with graphene-modified high-internal-phase emulsions (HIPEs) via a simple process of polymerization followed by carbonization. The morphology of the macroporous composites was observed and the verification of structural and functional groups were verified by scanning electron microscopy (SEM), and other characterizations techniques including Fourier transform infrared (FI-IR), X-ray photoelectron spectroscopy (XPS) spectra and Raman spectroscopy. The prepared porous carbon composites were applied to farmland water for the simultaneous adsorption of triazine herbicides and the conditions of extraction and desorption were optimized. Due to the π-π interaction and hydrophobic interaction between triazine herbicides and carbon composites, the maximum adsorption capacity of simazine, prometon and prometryn were 33.4, 34.5 and 33.8 µg g-1, respectively. Adsorption and desorption of triazine herbicides can be achieved in 10 min, and high-speed mass transfer was observed. The calibration curves of three triazine herbicides were linear (R2 ≥0.9996) in the range from 25.0 to 500.0 ng mL-1. The LOD of three triazine herbicides by using the proposed SPE-HPLC-DAD method were 2.5-5.6 ng mL-1. All the results suggest that these materials may be useful for more efficient hazardous residue separations.

2.
J Cell Physiol ; 234(10): 18985-18993, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30932200

RESUMO

NEAT1 is an important tumor oncogenic gene in various tumors. Nevertheless, its involvement remains poorly studied in cervical cancer. Our study explored the functional mechanism of NEAT1 in cervical cancer. NEAT1 level in several cervical cancer cells was quantified and we found NEAT1 was greatly upregulated in vitro. NEAT1 knockdown inhibited cervical cancer development through repressing cell proliferation, colony formation, capacity of migration, and invasion and also inducing the apoptosis. For another, microRNA (miR)-133a was downregulated in cervical cancer cells and NEAT1 negatively modulated miR-133a expression. Subsequently, we validated that miR-133a functioned as a potential target of NEAT1. Meanwhile, SOX4 is abnormally expressed in various cancers. SOX4 was able to act as a downstream target of miR-133a and silencing of SOX4 can restrain cervical cancer progression. In addition, in vivo assays were conducted to prove the role of NEAT1/miR-133a/SOX4 axis in cervical cancer. These findings implied that NEAT1 served as a competing endogenous RNA to sponge miR-133a and regulate SOX4 in cervical cancer pathogenesis. To sum up, it was implied that NEAT1/miR-133a/SOX4 axis was involved in cervical cancer development.

3.
J Cell Physiol ; 234(8): 13353-13360, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30613967

RESUMO

Long non-coding RNAs have been reported to play crucial roles in tumorigenesis including cervical cancer. LINC00037 has been identified as a significant regulator in several cancers. Our study was aimed to investigate the function of LINC00037 in cervical cancer progression. LINC00037 was significantly downregulated in human cervical cancer cells (HeLa, HCC94, HT-3, Caski, and SiHa cells) compared with the ectocervical epithelial cells (End1/E6E7 cells). Overexpression of LINC00037 was able to inhibit cervical cancer cell proliferation, induce cell apoptosis, and restrain the cell migration/invasion capacity. Reversely, knockdown of LINC00037 exhibited an opposite process in vitro. mTOR has been recognized as an atypical serine/threonine kinase that is involved in regulating significant cellular functions. In our present study, we observed that the mTOR signaling pathway was strongly activated in human cervical cancer cells. Meanwhile, upregulation of LINC00037 contributed to the inactivation of mTOR signaling whereas downregulation of LINC00037 activated the pathway. Subsequently, in vivo animal models using SiHa cells were established and we proved that LINC00037 repressed cervical cancer progression via targeting the mTOR signaling pathway. All these findings implied that LINC00037 could regulate cervical cancer pathogenesis via mTOR signaling. In conclusion, a novel role of LINC00037 was manifested in cervical cancer progression.

4.
J Cell Physiol ; 234(6): 9756-9763, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30362566

RESUMO

Cervical cancer continues to be a major public health problem. Although long noncoding RNAs (lncRNAs) were involved in the initiation and progression of cancer, few studies focus on the lncRNAs in the cervical cancer. Here, we systematically studied the clinical information, transcriptome profiling, and methylation array data of cervical squamous cell carcinoma and endocervical adenocarcinoma that retrieved from genomic data commons (GDC). Compared with protein-coding genes, the expression levels of pseudogenes and lncRNAs were much lower. A total of 190 differentially expressed lncRNAs and 2,326 protein-coding genes were identified. Meanwhile, 269 differentially methylation regions (DMRs), where 16 lncRNAs were located, were figured out. Only one lncRNA, LINC00592, which was located in the DMRs, was also found differentially expressed. Several transcriptional regulation genes, such as ZNF20, ZNF441, ZNF573, and TMF1, were highly correlated with the expression of LINC00592, which illustrated its possible function on the transcription. Two microRNAs, which were both associated with tumor progression, can bind to LINC00592. Moreover, LINC00592 were also differentially expressed in other tumors. We proposed, with the help of various databases, that LINC00592 is a potential cancer-related lncRNA in cervical cancer and might activate the cancer progression through the regulation of transcription or structural integrity.

5.
Nanoscale ; 10(25): 11997-12002, 2018 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-29904771

RESUMO

Rational design and understanding of the intrinsic mechanism are critical to develop highly active and durable electrocatalysts. In this study, a series of bi-metallic boride catalysts based on Ni and Co were prepared, and their activities were evaluated. The synthesised Co-10Ni-B catalyst exhibited excellent activity for water splitting in a 1 M KOH electrolyte. The overpotential was 330 mV at a current density of 10 mA cm-2, better than previously reported mono-metallic borides and even IrO2. The synergistic effect of Co and Ni was proved by X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy. The facile formation of critical intermediates CoOOH and NiOOH during the catalytic processes and a significant increase in surface area owing to the introduction of a second metal into mono-metallic boride were attributed to the superior catalytic performance of catalysts for the oxygen evolution reaction. A Co-10Ni-B-sp catalyst with a higher surface area than the Co-10Ni-B catalyst was also synthesised to evaluate the effect of a high surface area on the catalytic activity. A lower overpotential of 310 mV at a current density of 10 mA cm-2 was achieved.

6.
Langmuir ; 34(12): 3661-3668, 2018 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-29502419

RESUMO

Reduced graphene oxide (RGO)-hybridized polymeric high-internal phase emulsions (RGO/polyHIPEs) with an open-cell structure and hydrophobicity have been successfully prepared using 2-ethylhexyl acrylate and ethylene glycol dimethacrylate as the monomer and the cross-linker, respectively. The adsorption mechanism and performance of this RGO/polyHIPEs to polycyclic aromatic hydrocarbons (PAHs) were investigated. Adsorption isotherms of PAHs on RGO/polyHIPEs show that the saturated adsorption capacity is 47.5 mg/g and the equilibrium time is 8 h. Cycling tests show that the adsorption capacity of RGO/polyHIPEs remains stable in 10 adsorption-desorption cycles without observable structure change in RGO/polyHIPEs. Moreover, the PAH residues in water samples after being purified by RGO/polyHIPEs are lower than the limit values in drinking water set by the European Food Safety Authority. These results demonstrate that the RGO/polyHIPEs have great potentiality in PAH removal and water purification.

7.
Gynecol Oncol ; 149(1): 181-187, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29525275

RESUMO

Long non-coding RNAs (lncRNAs), which have little or no protein-coding capacity, caught a particular interest since their potential roles in the cancer paradigm. As the most common cancer in women, cervical squamous cell carcinoma remains one of the leading causes of deaths from cancer. However, limited evidence is available to determine the role of lncRNAs in the prognosis of cervical squamous cell carcinoma. In this study, we collected lncRNA expression profiling to identify prognosis related lncRNAs for cervical squamous cell carcinoma from TCGA database. In addition, we developed a 15-lncRNA signature based risk score to comprehensively assess the prognostic function of lncRNA. Furthermore, we performed a ROC analysis to identify the optimal cut-off point for classification risk level of the patients. Univariate Cox regression models were used to assess the association between lncRNAs and prognosis of patients with cervical squamous cell carcinoma. A 15-lncRNA based risk score was developed based on the Cox co-efficient of the individual lncRNAs. The prognostic value of this risk score was validated in the complete set and internal testing set. In summary, a 15-lncRNA expression signature (BAIAP2-AS1, RP11-203J24.8, LINC01133, RP1-7G5.6, RP11-147L13.15, SERHL, CTC-537E7.3, RP11-440L14.1, RP11-131N11.4, ILF3-AS1, RP11-80H18.4, RP11-1096G20.5, CTD-2192J16.26, RP11-621L6.3, and RP11-571M6.18) were identified and validated which can predict cervical cancer patient survival. The potential functions of this 15-lncRNA expression signature and individual lncRNAs as prognostic targets of cervical cancer were revealed by this study. Furthermore, these findings may have important implications in the understanding of the potential therapeutic method for the cervical squamous cell carcinoma patients.


Assuntos
Carcinoma de Células Escamosas/genética , RNA não Traduzido/genética , Neoplasias do Colo do Útero/genética , Sequência de Bases , Feminino , Humanos , Prognóstico
8.
Arch Oral Biol ; 83: 76-84, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28732226

RESUMO

OBJECTIVE: Intermittent administration of parathyroid hormone (PTH) has been demonstrated to have anabolic effects on bone metabolism and is approved for use in the treatment of osteoporosis. This study evaluates the role of intermittent PTH administration on alveolar bone loss in streptozotocin (STZ)-induced diabetic rats. DESIGN: Fifty male Sprague Dawley rats were randomly divided into the following five groups: (1) a control group (saline placebo without ligature and STZ injection), (2) a PTH group (PTH administration without ligature and STZ injection), (3) an L group (saline placebo with ligature), (4) an L+STZ group (saline placebo with ligature and STZ injection), and (5) an L+STZ+PTH group (PTH administration with ligature and STZ injection). PTH was administered at 75µg/kg per dose four times a week for 28days. Subsequently, all rats were sacrificed, and their mandibles were extracted for micro-computed tomography (micro-CT) scanning, as well as histological and immunochemical evaluation. RESULTS: Micro-CT scanning demonstrated the anabolic effect of PTH on alveolar bone metabolism in STZ-induced diabetic rats (P<0.05), and histomorphometry indicated that PTH inhibited inflammation of the periodontium and increased the level of osteoblastic activity (P<0.05). Immunochemical evaluation showed that rats subjected to both ligature placement and STZ injection had the highest receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio and that PTH administration decreased this ratio. CONCLUSION: Intermittent systemic PTH administration effectively reduced alveolar bone loss and ameliorated the manifestation of experimental periodontitis in STZ-induced diabetic rats.


Assuntos
Perda do Osso Alveolar/prevenção & controle , Diabetes Mellitus Experimental/complicações , Hormônio Paratireóideo/administração & dosagem , Hormônio Paratireóideo/farmacologia , Periodontite/etiologia , Periodontite/prevenção & controle , Perda do Osso Alveolar/diagnóstico por imagem , Animais , Masculino , Maxila/diagnóstico por imagem , Osteoblastos/efeitos dos fármacos , Osteoclastos/efeitos dos fármacos , Osteoprotegerina/metabolismo , Periodontite/diagnóstico por imagem , Ligante RANK/metabolismo , Ratos , Ratos Sprague-Dawley , Estreptozocina , Microtomografia por Raio-X
9.
Int J Legal Med ; 131(3): 677-683, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28058573

RESUMO

Our previous work demonstrated that characteristic changes could occur in the anterior wrist and medial malleolus in electric deaths through the hand-to-foot electric circuit pathway in an electric shock rat model. However, whether the same phenomenon occurs in humans is unknown. The aim of the present retrospective study was to ascertain whether the anterior wrist and medial malleolus could also be selected as the promising and significant sites in electric death through the hand-to-foot circuit pathway. Nineteen human cases from the autopsy and one clinical survivor who sustained a severe electric shock through the hand-to-foot circuit pathway were analyzed. Additional ten autopsy patients who died from traffic accidents and sudden cardiac attacks were used as the control group. Histopathological changes in the soft tissues of the anterior wrist and medial malleolus in all autopsy patients, as well as the electric current pathway of the survivor, were observed. The results showed that the nuclear polarizations in the anterior wrist and medial malleolus soft tissues of the electric death were extremely noticeable as compared with the controls. The most severe electrical injury in the survivor occurred in the anterior wrist. These findings suggest that the soft tissues of the anterior wrist and/or the medial malleolus as the narrowest parts of the limbs could be used as the complementary sites for tissue selection and considered as necessary locations for examinations to assess the electric death in medicolegal identification.


Assuntos
Traumatismos do Tornozelo/patologia , Traumatismos por Eletricidade/patologia , Traumatismos do Punho/patologia , Adulto , Fenômenos Biofísicos , Estudos de Casos e Controles , Feminino , Humanos , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
10.
Int J Legal Med ; 131(2): 433-439, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27752779

RESUMO

Specific morphological changes may be absent in some cases of electrocution shocked by the voltage of 220 V or lower. In this study, we attempted to demonstrate that the anterior wrist and medial malleolus were the optimal sites with promising and significant changes in electric death through the hand-to-foot circuit pathway. We established an electric shock rat model and observed histopathologic changes in the anterior wrist and medial malleolus. The results showed that the current intensities in the left anterior wrist and right medial malleolus were remarkably higher than those in the other sites, and the nuclei long/short (L/S) axis ratios of the arterial endotheliocyte and the skeletal muscle cell in these two areas were significantly higher than those in other parts of the body. These findings suggested that the anterior wrist and/or medial malleolus soft tissues as the narrowest parts of the limbs could be used as promising and useful sites for the assessment of electrical shock death, especially in forensic pathologic evaluation.


Assuntos
Carpo Animal/patologia , Traumatismos por Eletricidade/patologia , Tarso Animal/patologia , Animais , Aorta Abdominal/patologia , Artérias/patologia , Células Endoteliais/patologia , Patologia Legal , Modelos Animais , Músculo Esquelético/patologia , Ratos Sprague-Dawley
11.
Cell Biol Int ; 41(1): 84-92, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27778412

RESUMO

Lipopolysaccharide (LPS) plays an important role in bone resorption, which involves numerous cytokines through various signaling pathways. RANKL and interleukin (IL)-6 are two important cytokines that are involved in bone remodeling. The aim of this study was to evaluate the effect of LPS on RANKL and IL-6 gene expression, the relationship of RANKL and IL-6, and the role of extracellular signal-regulated kinases 1/2 (ERK1/2) on IL-6 secretion induced by LPS in MLO-Y4 cells. The cells were stimulated by LPS at different concentrations (1, 10, 100, 500, and 1000 ng/mL) for different durations (0.5, 1, 2, 4, and 8 h and 0.5, 1, 1.5, 2, and 4 h), and the mRNA expressions of RANKL and IL-6 were determined by PCR. In the presence of 100 ng/mL LPS at different time points (0.5, 1, 1.5, 2, and 4 h), IL-6 secretion and ERK1/2 phosphorylation in the cells were determined by ELISA and western blotting, respectively. STAT3 phosphorylation in cells simulated by 100 ng/mL LPS at different time points (0.5, 1, 2, 4, and 8 h) was assessed by western blotting. We found that LPS significantly up-regulated RANKL expression and activated the ERK1/2 pathway to induce IL-6 mRNA expression and protein synthesis in MLO-Y4 cells. However, the increased IL-6 was blocked by pre-treatment of MLO-Y4 cells with the ERK1/2 inhibitor U0126 (10 µM), and the enhanced RANKL was blocked by the STAT3 inhibitor S3I-201 (100 µM). Our results indicate that LPS up-regulates osteocyte expression of RANKL and IL-6, and the increased RANKL is associated with the up-regulation of IL-6, which involves the ERK1/2 pathway.


Assuntos
MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Interleucina-6/metabolismo , Lipopolissacarídeos/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Ligante RANK/genética , Regulação para Cima/efeitos dos fármacos , Animais , Western Blotting , Ativação Enzimática/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Interleucina-6/genética , Camundongos Transgênicos , Fosforilação/efeitos dos fármacos , Ligante RANK/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Fator de Transcrição STAT3/metabolismo
12.
Forensic Sci Res ; 2(1): 46-49, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-30483619

RESUMO

Intramedullary schwannoma of the upper cervical spinal cord is rarely reported in forensic medicine. We herein report a case involving a patient who died of compression from an intramedullary schwannoma in the upper cervical spinal cord. A 30-year-old man initially presented with a five-day history of pain in the left chest that progressed to weakening in the left arm. Although the patient was treated with analgesic poultices, he developed inspiratory dyspnoea and died while working the next day without having undergone any medical imaging examination or surgical treatment. Anatomical and histopathological examinations revealed an intramedullary schwannoma in the left cervical spinal cord (C3-C5) underneath the spinal nerve root. The cause of death might have been asphyxia secondary to the tumour, which interfered with the nerve function in the respiratory muscles. This finding suggests that an autopsy is essential for pathologists and medicolegists to comprehensively undertake their due obligation to obtain "the first evidence", especially when there is a lack of directly related evidence. As part of the central nervous system, the spinal cord could be systematically included in a routine pathological autopsy in some cases.

13.
Sci Rep ; 6: 32855, 2016 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-27619006

RESUMO

The onion maggot, Delia antiqua, is a devastating pest of liliaceous crops and current control measures fail to avert pesticide residues, threats to agroecosystem, and costly expenditures. Insect growth regulators (IGRs) are used as trypetid pest chemosterilants for their suppression on adult fertility and fecundity, but their effects on onion flies are unknown. Here, three IGRs (lufenuron, cyromazine, pyriproxyfen) were incorporated into baits to evaluate their effects on onion fly survival, fecundity, fertility, susceptibility of adults in different ages and offspring development. Lufenuron and cyromazine did not affect survival of new-emerged adults, but lufenuron inhibited adult fertility without affecting fecundity, and cyromazine reduced fertility and fecundity. Differently, pyriproxyfen enhanced fecundity within 10 days after treatment, while it reduced adult survival without affecting fertility. The fertility of younger adults was affected by lufenuron and cyromazine whereas the fecundity was affected with cyromazine and pyriproxyfen. For offspring of onion flies treated with lufenuron or cyromazine, most of larvae died within 5 days after hatch, but surviving larvae pupated and emerged normally. Pyriproxyfen did not affect offspring larval survival or pupation but affected pupal emergence. Thus, lufenuron and cyromazine could be potential chemosterilants for onion flies.


Assuntos
Benzamidas/farmacologia , Dípteros/crescimento & desenvolvimento , Hormônios Juvenis/farmacologia , Piridinas/farmacologia , Triazinas/farmacologia , Animais , Dípteros/efeitos dos fármacos , Fertilidade/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Praguicidas/farmacologia , Pupa/crescimento & desenvolvimento
14.
Biol Trace Elem Res ; 168(2): 335-9, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26043914

RESUMO

Proper trace element level is crucial for the organs in maintaining normal physiological functions. Multiple organ failure (MOF) might be added to critically ill patients due to a lack of trace elements. Alterations of trace element levels in brain, heart, liver, and kidney after severe trauma, however, have been little studied so far. In this study, tissue samples of the frontal cortex of the brain, interventricular septum of the heart, right lobe of the liver, and upper pole of the kidney were obtained from forensic autopsies, of which 120 cases died during the 5th to 15th day of hospitalization, whereas the trauma death group and 43 cases immediately died due to severe craniocerebral trauma as the control group. Copper (Cu), iron (Fe), zinc (Zn), and selenium (Se) were quantified by inductively coupled plasma atomic emission spectrophotometry (ICP-AES). Cu, Fe, Zn, and Se concentrations in the brain, heart, liver, and kidney in the trauma group decreased dramatically (p<0.05) compared to the control group. The incidence of secondary infection and multiple organ failure (MOF) in the trauma death group were 78.33 and 29.17%, respectively. The concentrations of all elements exhibited a significant correlation with secondary infection and MOF (p<0.01). Our data suggest that low concentrations of Cu, Fe, Zn, and Se in pivotal organs may contribute to the incidence of secondary infection and MOF after severe trauma, which to some extent results in death.


Assuntos
Coinfecção/sangue , Insuficiência de Múltiplos Órgãos/sangue , Oligoelementos/análise , Ferimentos e Lesões/sangue , Adulto , Autopsia , Encéfalo/metabolismo , Coinfecção/mortalidade , Cobre/análise , Feminino , Hospitalização , Humanos , Ferro/análise , Rim/metabolismo , Fígado/metabolismo , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/mortalidade , Miocárdio/metabolismo , Selênio/análise , Espectrofotometria Atômica , Distribuição Tecidual , Ferimentos e Lesões/mortalidade , Zinco/análise
15.
Arch Oral Biol ; 60(5): 789-98, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25766472

RESUMO

OBJECTIVE: 5-Hydroxytryptophan (5-HTP) is the precursor of serotonin and 5-HTP has been widely used as a dietary supplement to raise serotonin level. Serotonin has recently been discovered to be a novel and important player in bone metabolism. As peripheral serotonin negatively regulates bone, the regular take of 5-HTP may affect the alveolar bone metabolism and therefore influence the alveolar bone loss induced by periodontitis. The aim of this study was to investigate the effect of 5-HTP on alveolar bone destruction in periodontitis. DESIGN: Male Sprague-Dawley rats were randomly divided into the following four groups: (1) the control group (without ligature); (2) the 5-HTP group (5-HTP at 25 mg/kg/day without ligature); (3) the L group (ligature+saline placebo); and (4) the L+5-HTP group (ligature+5-HTP at 25 mg/kg/day). Serum serotonin levels were determined by ELISA. The alveolar bones were evaluated with micro-computed tomography and histology. Tartrate-resistant acid phosphatase staining was used to assess osteoclastogenesis. The receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) expression in the periodontium as well as the interleukin-6 positive osteocytes were analysed immunohistochemically. RESULTS: 5-HTP significantly increased serum serotonin levels. In rats with experimental periodontitis, 5-HTP increased alveolar bone resorption and worsened the micro-structural destruction of the alveolar bone. 5-HTP also stimulated osteoclastogenesis and increased RANKL/OPG ratio and the number of IL-6 positive osteocytes. However, 5-HTP treatment alone did not cause alveolar bone loss in healthy rats. CONCLUSION: The present study showed that 5-HTP aggravated alveolar bone loss, deteriorated alveolar bone micro-structure in the presence of periodontitis, which suggests 5-HTP administration may increase the severity of periodontitis.


Assuntos
5-Hidroxitriptofano/administração & dosagem , 5-Hidroxitriptofano/farmacologia , Perda do Osso Alveolar/induzido quimicamente , Periodontite/complicações , Administração Oral , Perda do Osso Alveolar/diagnóstico por imagem , Animais , Progressão da Doença , Ensaio de Imunoadsorção Enzimática , Técnicas Imunoenzimáticas , Interleucina-6/metabolismo , Masculino , Osteoclastos/efeitos dos fármacos , Osteócitos/efeitos dos fármacos , Ligante RANK/metabolismo , Ratos , Ratos Sprague-Dawley , Receptor Ativador de Fator Nuclear kappa-B/metabolismo , Serotonina/sangue , Microtomografia por Raio-X
16.
Molecules ; 19(11): 17682-96, 2014 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-25365294

RESUMO

The effects of extraction time, temperature, pressure and different concentration of ethanol and their interactions on the yields of amentoflavone, quercetin and ginkgetin extracted from Taxus chinensis by supercritical CO2 were investigated by using a central composite design (CCD). An CCD experimental design with four factors and five levels was used to optimize the extraction parameters. Ultra performance liquid chromatography (UPLC) was used to analyze the content of the tree components in the extracts. Experimental results show that the main effects of factors and their interactions are significant on the yields (p < 0.05). The optimal extraction conditions were established for the three compounds: yield of 4.47 mg/g for amentoflavone at 48 °C, 25 MPa, 2.02 h and 78.5% ethanol, 3.73 mg/g for quercetin at 46 °C, 24 MPa, 2.3 h, 82% ethanol and 3.47 mg/g for ginkgetin at 48 °C, 20 MPa, 2.38 h, 82% ethanol, respectively.


Assuntos
Biflavonoides/química , Dióxido de Carbono/química , Extratos Vegetais/química , Quercetina/química , Solventes/química , Taxus/química , Cromatografia Líquida de Alta Pressão/métodos , Etanol/química , Pressão , Temperatura
17.
Molecules ; 19(10): 15452-67, 2014 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-25264832

RESUMO

The aqueous extract from Carya cathayensis Sarg. exocarp was centrifuged, filtered, and separated into 11 elution fractions by X-5 macroporous resin chromatography. A phenolic compound, 4,8-dihydroxy-1-tetralone (4,8-DHT) was isolated from the fractions with the strongest phytotoxicity by bioassy-guided fractionation, and investigated for phytotoxicity on lettuce (Latuca sativa L.), radish (Raphanus sativus L.), cucumber (Cucumis sativus L.), onion (Allium cepa L.) and wheat (Triticum aestivum L.). The testing results showed that the treatment with 0.6 mM 4,8-DHT could significantly depress the germination vigor of lettuce and wheat, reduce the germination rate of lettuce and cucumber, and also inhibit radicle length, plumule length, and fresh weight of seedlings of lettuce and onion, but could significantly promote plumule length and fresh weight of seedlings of cucumber (p < 0.05). For the tested five plants, the 4,8-DHT was the most active to the seed germination and seedling growth of lettuce, indicating that the phytotoxicity of 4,8-DHT had the selectivity of dosage, action target (plant type) and content (seed germination or seedling growth).


Assuntos
Carya/química , Extratos Vegetais/farmacologia , Tetralonas/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Germinação/efeitos dos fármacos , Alface/efeitos dos fármacos , Alface/crescimento & desenvolvimento , Ressonância Magnética Nuclear Biomolecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Tetralonas/química , Tetralonas/isolamento & purificação , Tetralonas/toxicidade
18.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 22(3): 681-6, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24989276

RESUMO

This study was aimed to investigate the inducing-apoptosis effect of brucine on human monocytic leukemia cell line THP-1 cells and its possible mechanism. The inhibition effect of brucine on growth of THP-1 cells was measured by CCK-8 method. Morphological changes of THP-1 cells treated with brucine was detected by acridine orange/ethidium bromide (AO/EB)double staining. Annexin-V/PI double labeling method was used to assay the apoptosis rate of THP-1 cells. The effect of brucine on THP-1 cell cycle distribution was detected by PI single staining. RT-PCR was used to detect the expression of BCL-2 and BAX. The results showed that the brucine could inhibit the THP-1 cell growth in concentration and time-dependent manners at the range of 50 to 400 µg/ml. The cells stained with AO/EB revealed that the brucine induced the nuclear chromatin condensation. After the THP-1 cells were treated with brucine of 400µg/ml for 48 hours, most nucleic were stained as orange-red, and condensed, displaying the late apoptotic cell morphology. Annexin-V/PI detection showed that brucine could induce apoptosis of THP-1 cells in a concentration-dependent manner. Compared with the control group, more cells in brucine-treated group were arrested at G0/G1 phase in a concentration-dependent manner. RT-PCR detection revealed that the expression of BCL-2 was down-regulated strikingly and BAX was up-regulated. It is concluded that brucine can efficiently inhibit cell growth and block THP-1 cells in G0/G1 phase. The mechanism of THP-1 cell apoptosis induced by brucine may be related to the inhibition of BCL-2 and activation of BAX.


Assuntos
Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Estricnina/análogos & derivados , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Estricnina/farmacologia , Proteína X Associada a bcl-2/metabolismo
19.
Mol Cell Endocrinol ; 384(1-2): 155-64, 2014 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-24508663

RESUMO

The identification of the neuronal control of bone remodeling has become one of the many significant recent advances in bone biology. Cholinergic activity has recently been shown to favor bone mass accrual by complex cellular regulatory networks. Here, we identified the gene expression of the muscarinic and nicotinic acetylcholine receptors (m- and nAChRs) in mice tibia tissue and in osteocytic MLO-Y4 cells. Acetylcholine, which is a classical neurotransmitter and an osteo-neuromediator, not only influences the mRNA expression of the AChR subunits but also significantly induces the proliferation and viability of osteocytes. Moreover, acetylcholine treatment caused the reciprocal regulation of RANKL and OPG mRNA expression, which resulted in a significant increase in the mRNA ratio of RANKL:OPG in osteocytes via acetylcholine receptors. The expression of neuropeptide Y and reelin, which are two neurogenic markers, was also modulated by acetylcholine via m- and nAChRs in MLO-Y4 cells. These results indicated that osteocytic acetylcholine receptors might be a new valuable mediator for cell functions and even for bone remodeling.


Assuntos
Acetilcolina/farmacologia , Osteócitos/efeitos dos fármacos , Subunidades Proteicas/genética , RNA Mensageiro/genética , Receptores Muscarínicos/genética , Receptores Nicotínicos/genética , Animais , Moléculas de Adesão Celular Neuronais/genética , Moléculas de Adesão Celular Neuronais/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Regulação da Expressão Gênica , Camundongos , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Neuropeptídeo Y/genética , Neuropeptídeo Y/metabolismo , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteócitos/citologia , Osteócitos/metabolismo , Osteoprotegerina/genética , Osteoprotegerina/metabolismo , Subunidades Proteicas/metabolismo , Ligante RANK/genética , Ligante RANK/metabolismo , RNA Mensageiro/metabolismo , Receptores Muscarínicos/metabolismo , Receptores Nicotínicos/metabolismo , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo , Transdução de Sinais , Tíbia/citologia , Tíbia/efeitos dos fármacos , Tíbia/metabolismo
20.
Front Med ; 8(1): 96-100, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24464488

RESUMO

Lymph node metastasis has an important effect on prognosis of patients with ovarian cancer. Moreover, the impact of para-aortic lymph node (PAN) removal on patient prognosis is still unclear. In this study, 80 patients were divided into groups A and B. Group A consisted of 30 patients who underwent PAN + pelvic lymph node (PLN) dissection, whereas group B consisted of 50 patients who only underwent PLN dissection. Analysis of the correlation between PAN clearance and prognosis in epithelial ovarian cancer was conducted. Nineteen cases of lymph node metastasis were found in group A, among whom seven cases were positive for PAN, three cases for PLN, and nine cases for both PAN and PLN. In group B, 13 cases were positive for lymph node metastasis. Our study suggested that the metastatic rate of lymph node is 40.0%. Lymph node metastasis was significantly correlated with FIGO stage, tumor differentiation, and histological type both in groups A and B (P < 0.05). In groups A and B, the three-year survival rates were 77.9% and 69.0%, and the five-year survival rates were 46.7% and 39.2%, respectively. However, the difference was not statistically significant (P > 0.05). The three-year survival rates of PLN metastasis in groups A and B were 68.5% and 41.4%, and the five-year survival rates were 49.7% and 26.4%, respectively. Furthermore, PLN-positive patients who cleared PAN had significantly higher survival rate (P = 0.044). In group A, the three-year survival rates of positive and negative lymph nodes were 43.5% and 72.7%, and the five-year survival rates were 27.2% and 58.5%, respectively. The difference was statistically significant (P = 0.048). Cox model analysis of single factor suggested that lymph node status affected the survival rate (P < 0.01), which was the death risk factor. Consequently, in ovarian carcinoma cytoreductive surgery, resection of the para-aortic lymph node, which has an important function in clinical treatment and prognosis of patients with ovarian cancer, is necessary.


Assuntos
Excisão de Linfonodo , Neoplasias Ovarianas/patologia , Aorta Abdominal , Feminino , Humanos , Excisão de Linfonodo/mortalidade , Excisão de Linfonodo/estatística & dados numéricos , Metástase Linfática/patologia , Pessoa de Meia-Idade , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/mortalidade , Prognóstico , Modelos de Riscos Proporcionais , Análise de Sobrevida
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