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1.
Artigo em Inglês | MEDLINE | ID: mdl-34865214

RESUMO

OBJECTIVE: To explore independent factors influencing the risk of lower extremity deep vein thrombosis during the postoperative period in patients with gynecological malignancies by constructing a predictive model. METHODS: In our study, we collected 573 patients with gynecological malignancies in the postoperative period between September 2016 and September 2020, who were divided into a modeling (n=402) and verification group (n=171) according to a ratio of 7:3. Univariate and multivariate regression analyses were used to determine independent factors influencing deep vein thrombosis(DVT). A nomogram model was created and a risk score was calculated. RESULTS: Multivariate regression analysis showed that the independent factors affecting DVT among these patients included age, hyperlipidemia, abnormal uterine bleeding, degree of anemia, D-dimer, operation time, and intraoperative blood loss. By incorporating these factors into a nomogram, we determined that the C-index and calibration curve of the two groups both showed that the model distinguishes and fits well. Further comparing between the high- and low-risk groups, we found that the model has favorable predictive performance. CONCLUSIONS: The predictive nomogram for the risk of DVT in patients with gynecological malignancies in the postoperative period demonstrated good calibration and recognition accuracy. Further independent research is necessary to verify our results.

2.
Glob Chang Biol ; 2021 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-34935251

RESUMO

Direct measuring of CO2  flux remains challenging for global lakes. The traditional sampling and gas transfer models used to estimate lake CO2  fluxes are variable and uncertain, and ice-covered periods are often excluded from the annual carbon budget. Here, the first longtime (2013-2017) direct measurement of CO2  flux by eddy covariance system over the largest saline lake (Qinghai lake) in the Qinghai-Tibet Plateau (QTP) revealed that ice-covered period draws large amounts of CO2 from the atmosphere (-0.87 ± 0.38 g C m-2 d-1 ), a value more than twice the CO2  flux rate during the ice-free period (-0.41 ± 0.35 g C m-2 d-1 ). The total CO2 uptake by all saline lakes on the QTP was estimated to -10.28 ± 1.65 Tg C yr-1 , an equivalent to approximately one third of the net terrestrial ecosystems carbon sink in QTP. Our results indicate large sink for CO2 in winter is controlled by both seasonal hydrochemistry processes and lake ice absorption in saline lakes. This research also demonstrates decreasing CO2 uptake from the atmosphere by saline lakes on the QTP, which may turn carbon sinks to carbon sources with future warming.

3.
Front Oncol ; 11: 621917, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912696

RESUMO

Background: Concurrent chemoradiotherapy (CRT) is the preferred treatment strategy for inoperable esophageal cancer (EC). However, the effect of CRT needs to be improved. Methods: This study comprehensively analyzed targeted agents combined with CRT for the treatment of EC by a network meta-analysis. The search was performed in public databases from incipient to 5 August 2021. Randomized controlled trials comparing the effect of targeted agents combined with CRT and CRT alone on EC patients were included. Results: Ten studies were included. For progression-free survival (PFS), nivolumab (67.4%) and erlotinib (64.6%) had advantages based on Cox analysis. Regarding the frequency of PFS, cetuximab (OR: 1.39; 95% CI: 1.01, 1.91; p=0.042) and nivolumab (OR: 1.81; 95% CI: 1.34, 2.44; p<0.01) were significantly superior to the control. For overall survival (OS), nivolumab (71.6%) in Cox analysis and nimotuzumab (69.7%) in frequency analysis were found to have relative advantages. Nimotuzumab combined with CRT was significantly better than the control with regard to endoscopic and the pathologic complete response (epCR; OR: 2.81; 95% CI: 1.28, 6.14; p=0.011) and objective response rate (ORR; 4.71; 95% CI: 1.45, 15.29; p=0.008). The targeted drugs were not associated with significant SEA risk. Conclusion: In conclusion, compared to CRT alone, cetuximab and nivolumab combined with CRT were found to significantly improve the PFS rate only based on the frequency results. However, there was no benefit in terms of OS. For epCR and ORR, nimotuzumab was better than the blank control. Considering the limitations in this study, more well-designed RCTs are needed in the future to validate the results.

4.
Virus Res ; 309: 198657, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34919970

RESUMO

BACKGROUND: The relationship between the serum transforming growth factor (TGF)-ß level and HBsAg loss has not been clearly elaborated in patients with chronic hepatitis B (CHB). METHODS: Two cohorts of patients with CHB were studied. Cohort A: A total of 207 hepatitis B e antigen (HBeAg)-negative CHB patients who finished ≥1 year nucleos(t)ide analogue monotherapy and sequentially received PEGylated interferon treatment for less than 96 weeks were included. Cohort B: Forty HBeAg-positive patients who initially received entecavir therapy for at least 96 weeks were included. Their viral markers and serum TGF-ß levels were measured at different time points during therapy. RESULTS: The levels of serum TGF-ß and HBsAg (0-24 W) were significantly lower in the patients who had HBsAg< 0.05 IU/mL at 48 weeks than in patients who did not in cohort A. We got the same results when we further divided the patients into subgroups according to the initial HBsAg cut-off values (1000 IU/mL, 100 IU/mL, 50 IU/mL) in cohort A. However, HBeAg seroconversion did not lead to the downregulation of TGF-ß levels. The levels of serum TGF-ß were significantly correlated with HBsAg quantitation in cohort A (12-24 W) but not in cohort B (0-48 W). The levels of TGF-ß at week 12 could be used as an early index to predict a functional cure (AUC=0.818) as well as the levels of HBsAg itself (AUC=0.882) in HBeAg-negative chronic hepatitis B patients treated with PEGylated interferon. CONCLUSIONS: The levels of serum TGF-ß were significantly associated with HBsAg loss but not with HBeAg seroconversion and could be used as an early index to predict a functional cure in CHB patients treated with PEGylated interferon.

5.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 37(5): 506-510, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34816662

RESUMO

Objective: To investigate the synergistic effects of magnolol and gefitinib on non-small cell lung cancer A549 cells. Methods: A549 cells were treated with Magnolol (6.25~500 µmol/L) or gefitinib (6.25~500 µmol/L) for 24 h, respectively, and the cell viability was detected by cell counting Kit-8 (CCK-8) experiment (n=3). Magnolol 100 µmol/L and gefitinib 5 µmol/L were selected in the following experiments (n=3, 24 h). Control group, magnolol group, gefitinib group and magnolol+gefitinib group were set up for factorial analysis. Colony formation experiment was applied to detect the cell proliferation. Western blot was used to detect protein expressions. Flow cytometry was applied to test cell apoptosis and sorting CD44+ and CD133+ cells. Results: Compared with the control group, the colony formation rate of Magnolol or Gefitinib groups was decreased significantly (P<0.05); the apoptosis rate was increased significantly (P<0.05); the number of CD44+ and CD133+ cells was reduced significantly (P<0.05); the expressions of Ki67, PCNA, and stem cell marker proteins SOX2 and OCT4 were down-regulated (P<0.05); and the ratio of Bax/Bcl-2 was increased significantly (P<0.05). Compared with the Magnolol group or Gefitinib group, the Magnolol+Gefitinib group further promoted the above changes (P<0.05), and the apoptosis rate, the ratio of Bax/Bcl-2, SOX2 and OCT4 all showed interactions between magnolol and gefitinib (P<0.05). Conclusion: Magnolol and gefitinib promote the apoptosis of A549 cells and inhibit its stem cell-like properties, and the effect of the two combined is better than separated administration. Magnolol and gefitinib have interactive effects on A549 cells.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Células A549 , Apoptose , Compostos de Bifenilo , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Linhagem Celular Tumoral , Proliferação de Células , Gefitinibe/farmacologia , Humanos , Lignanas , Neoplasias Pulmonares/tratamento farmacológico
6.
World J Clin Cases ; 9(26): 7811-7817, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34621831

RESUMO

BACKGROUND: Pediatric temporal fistulae are rarely reported in the literature. Dissemination of these cases can help inform future diagnosis and effective treatment. CASE SUMMARY: Three pediatric patients came to the clinic due to repeated infections of the skin and soft tissue of the temporal area. One patient presented with a temporal fistula that penetrated the temporal bone and reached the dura mater. Another patient presented with a temporal fistula that penetrated into the temporal muscle fascia. The third patient presented with a fistula that penetrated the lateral wall of the orbit and entered the orbit. All patients underwent surgical fistula resection informed by preoperative computed tomography (CT) evaluation. Histopathological evaluation was also performed. All three patients were surgically treated successfully. Histopathological evaluations confirmed the fistula diagnoses in all three cases. CONCLUSION: For patients who have temporal fistulae with repeated infections, surgical treatment should be performed as soon as possible to prevent serious complications. CT can be very useful for preoperative evaluation. B-mode ultrasound examination and evaluation also have a certain auxiliary role.

7.
Zhongguo Zhen Jiu ; 41(10): 1084-8, 2021 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-34628739

RESUMO

OBJECTIVE: To observe the efficacy difference between conventional needling depth and deep needling for dyspepsia after ischemic stroke. METHODS: A total of 120 patients with dyspepsia after ischemic stroke were randomized into an observation group (60 cases, 4 cases dropped off) and a control group (60 cases, 3 cases dropped off). Basic treatment was given in the both groups. In the observation group, deep needling was applied at Zhongwan (CV 12), Tianshu (ST 25) and Liangmen (ST 21) for 60-70 mm, after even reinforcing-reducing manipulation of lifting-thrusting technique, the needles were withdrew to 35-50 mm. In the control group, the same acupoints as the observation group were selected and punctured for 25 mm. The needles were retained for 30 min, once a day, 6 times a week for 2 weeks in the both groups. The dyspepsia TCM symptom score was observed before treatment, 1 day and 1, 2 weeks into treatment, and the clinical efficacy was evaluated 2 weeks into treatment in the both groups. RESULTS: The effective rate was 92.9% (52/56) in the observation group, which was superior to 78.9% (45/57) in the control group (P<0.01). Compared before treatment, the total scores and sub-item scores of dyspepsia TCM symptom of 1, 2 weeks into treatment were decreased in the both groups (P<0.01, P<0.05). The total scores and abdominal fullness scores of dyspepsia TCM symptom of each time point into treatment and hiccup score of 2 weeks into treatment in the observation group were lower than those in the control group (P<0.01, P<0.05). The total scores of dyspepsia TCM symptom 1, 2 weeks into treatment in the mild patients and each time point into treatment in the moderate patients of the observation group were lower than those in the control group (P<0.01). CONCLUSION: Conventional needling depth and deep needling can both improve the clinical symptoms in patients with dyspepsia after ischemic stroke, and deep needling has faster and better efficacy.


Assuntos
Terapia por Acupuntura , Isquemia Encefálica , Dispepsia , AVC Isquêmico , Acidente Vascular Cerebral , Dispepsia/terapia , Humanos , Acidente Vascular Cerebral/complicações , Acidente Vascular Cerebral/terapia
8.
Shanghai Kou Qiang Yi Xue ; 30(4): 367-373, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34693428

RESUMO

PURPOSE: To explore the effect of enamel matrix proteins(EMPs) on osteogenesis and adipogenesis of stem cells from human exfoliated deciduous teeth SHED), and explore its molecular mechanism. METHODS: SHEDs were used to detect the expression of its surface antigens CD73, CD146, CD34 and CD45 by flow cytometry. SHED was induced by OB osteogenic induction liquid, and then the osteogenic differentiation ability was measured by alizarin red staining. SHEDs were divided into 4 groups, NC group had invalid sequence shRNA interfered with SHED, EMPs group had invalid sequence shRNA interfered with SHED. Then 100 µg/L EMPs was used to interfere with SHED. In miR-32 inhibitor group, miR-32 shRNA plasmid was used to interfere with SHED; while in EMPs+miR-32 inhibitor group, 100 µg/L EMP was used to intervene SHED after silencing miR-32. QPCR was used to detect the expression of miR-32, dentin sialophosphoprotein (DSPP), dentin matrix protein 1, DMP-1, peroxisome proliferators-activated receptor γ (PPARγ) and CCAAT enhancer binding protein α (C/EBPα) gene expression; Western blot was used to detect the expression of DSPP, DMP-1, PPARγ and C/EBPα protein expression; Alizarin red staining was used to detect SHED osteogenic capacity; Oil red O staining was used to detect adipogenetic capacity of SHED. RESULTS: The results of flow cytometry showed that SHED had positive expression of CD146 and CD73, and negative expression of CD34 and CD45, which was consistent with the characteristics of stem cell surface markers. Alizarin red staining and oil red O staining showed mineralized nodules and oil droplets increased significantly, consistent with the multi-directional differentiation characteristics of stem cells. Compared with NC group, the expression of miR-32 gene in EMPs group was significantly increased(P<0.05), and the expression of miR-32 in miR-32 inhibitor group and EMPs+miR-32 inhibitor group was significantly decreased(P<0.05). Compared with NC group, the expression of DSPP and DMP-1, the number of mineralized nodules in EMPs group were significantly increased(P<0.05), the expression of PPARγ and C/EBPa and the number of lipid droplets were significantly decreased (P<0.05), while the result of miR-32 inhibitor group was the opposite (P<0.05). Compared with miR-32 inhibitor group, there was no significant difference in the expression of DSPP, DMP-1, PPARγ and C/EBPα, number of mineralized nodules and oil droplets in EMPs+miR-32 inhibitor group(P>0.05). Compared with EMPs group, the expression of DSPP and DMP-1 and the number of mineralized nodules in EMPs+miR-32 inhibitor group were significantly reduced(P<0.05), while the expression of PPARγ and C/EBPα and the number of lipid droplets were significantly increased(P<0.05). CONCLUSIONS: EMPs can regulate osteogenic and adipogenic differentiation of SHED by promoting the expression of miR-32.


Assuntos
MicroRNAs , Osteogênese , Adipogenia/genética , Diferenciação Celular , Células Cultivadas , Proteínas do Esmalte Dentário , Polpa Dentária , Humanos , MicroRNAs/genética , Osteogênese/genética , Células-Tronco , Dente Decíduo
9.
Shanghai Kou Qiang Yi Xue ; 30(4): 389-393, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34693432

RESUMO

PURPOSE: This study investigated the effectiveness of orofacial myofunctional therapy(OMT) in improving facial morphology of children with obstructive sleep apnea (OSA) after adenotonsillectomy (AT). METHODS: Ten children aged from 4-7 years with persistent oral breathing for more than 1 month after adenotonsillectomy were chosen to receive orofacial myofunctional therapy. The patients were required to take photos before and after orofacial myofunctional therapy. In order to compare the soft changes before and after OMT treatment, twelve representative mark points were selected and used for proportion and angle measurements. Graphpad Prism 8 statistical software was used for statistical analysis, to compare the differences in facial morphology of patients before and after treatment. RESULTS: Compared with before OMT, a significant difference was found in the proportion of Sn-Ls/Sn-Stms(P=0.0002), Sn-Stms/Sn-Me'(P<0.05), as well as in the angle of Gs-Sn-Pos (P<0.05), nasolabial angle(P=0.0005), mentolabial angle (P=0.0026) after OMT treatment. CONCLUSIONS: Orofacial myofunctional therapy can be considered as an effective complementary treatment for OSA patients with oral breathing after adenotonsillectomy.


Assuntos
Apneia Obstrutiva do Sono , Tonsilectomia , Adenoidectomia , Criança , Face , Humanos , Terapia Miofuncional , Apneia Obstrutiva do Sono/terapia
10.
Neurology ; 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34706976

RESUMO

BACKGROUND AND OBJECTIVES: Few biochemical markers have been identified in spinocerebellar ataxia type 2 (SCA2). This study aimed to determine the levels of neurofilament light (NfL) in patients with SCA2 and identify whether they were associated with disease severity. METHODS: Participants were recruited from one medical center in China, and individuals with SCA2 were genetically diagnosed. NfL levels were assessed using the single molecule array method. Disease severity was evaluated using the Scale for the Assessment and Rating of Ataxia (SARA), the International Cooperative Ataxia Rating Scale (ICARS), and the Inventory of Non-Ataxia Symptoms (INAS). Cerebellum and brainstem volumes were calculated using neuroimaging measurements. We used Pearson's correlation and partial correlation for correlation analyses. RESULTS: Forty-nine manifest patients with SCA2, 10 preclinical individuals with SCA2 and 92 controls were enrolled. A high consistency was identified between serum and CSF NfL (r = 0.868, p < 0.0001). In individuals with SCA2, levels of serum NfL were associated with disease severity (SARA, r = 0.425, p = 0.003; ICARS, r = 0.383, p = 0.009; INAS, r = 0.390, p = 0.007; cerebellum volume, r = - 0.393, p = 0.024) after adjustment for age. NfL levels were higher close to the expected age of onset in preclinical individuals with SCA2 (R 2 = 0.43, p = 0.04). DISCUSSION: Levels of serum NfL were correlated with disease intensity in individuals with SCA2, and were higher close to the estimated age of onset in preclinical SCA2. Therefore, NfL is a potential serum biomarker of disease severity in SCA2. CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that elevated NfL levels are associated with disease severity in individuals with SCA2.

11.
Clin Ophthalmol ; 15: 4097-4108, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675477

RESUMO

Purpose: To evaluate the safety and efficacy of dexamethasone intravitreal implant 0.7 mg (DEX) compared with laser photocoagulation in patients with diabetic macular edema (DME). Patients and Methods: This Phase 3, multicenter, randomized, efficacy evaluator-masked, parallel-group, 12-month clinical study enrolled adults in China and the Philippines with reduced visual acuity secondary to fovea-involved DME in the study eye. Participants were randomized 1:1 to study eye treatment with laser photocoagulation every 3 months as needed (n = 139) or DEX every 5 months (n = 145). The main efficacy measures were best-corrected visual acuity (BCVA), central retinal thickness (CRT), and leakage area. The primary endpoint was the average change in BCVA from baseline over 12 months (area-under-the-curve method). Preplanned subgroup analyses evaluated outcomes in Chinese patients. Results: Mean average change in BCVA from baseline during the study (letters) was 4.3 with DEX (n = 145) versus 1.4 with laser (n = 127) overall (P = 0.001) and 4.6 with DEX (n = 129) versus 0.6 with laser (n = 113) in Chinese patients (P < 0.001). At Month 12, mean change in CRT from baseline was -209.5 µm with DEX versus -120.3 µm with laser (P < 0.001) and mean change in total leakage area from baseline was -8.367 mm2 with DEX versus -0.637 mm2 with laser (P < 0.001). The most common treatment-emergent adverse events in the DEX group were increased intraocular pressure and cataract. Conclusion: DEX administered every 5 months provided significantly greater improvement in BCVA, CRT, and total leakage area compared with laser treatment. DEX demonstrated an acceptable safety profile, consistent with an intraocular corticosteroid, and similar to that reported in completed global registration studies.

12.
Opt Lett ; 46(20): 5181-5184, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34653146

RESUMO

In this Letter, we propose a fabrication technique based on femtosecond laser secondary direct writing (FsLSDW) that allows us to statically reset the beam-splitting ratio of directional couplers. By modifying the interaction region with a second inscription, the coupling coefficient of the reconstructed devices can be indeed changed continuously within the range of 0.49-2.1 rad/mm, thus enabling a complete tunability of the reconstructed splitting ratio from zero to full power transfer between the waveguides. This powerful reconstruction capability facilitates the arbitrary reset of an imperfect device, from any initial splitting ratio to the correct one. In the future, such static control method could potentially solve the fabrication error problem in the manufacturing of high-fidelity large-scale integrated photonic quantum chips.

13.
Aging (Albany NY) ; 13(17): 21155-21190, 2021 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-34517344

RESUMO

In this study, we investigated the role of embryonic gene Cripto-1 (CR-1) in hepatocellular carcinoma (HCC) using hepatocyte-specific CR-1-overexpressing transgenic mice. The expression of truncated 1.7-kb CR-1 transcript (SF-CR-1) was significantly higher than the full-length 2.0-kb CR-1 transcript (FL-CR-1) in a majority of HCC tissues and cell lines. Moreover, CR-1 mRNA and protein levels were significantly higher in HCC tissues than adjacent normal liver tissues. Hepatocyte-specific over-expression of CR-1 in transgenic mice enhanced hepatocyte proliferation after 2/3 partial hepatectomy (2/3 PHx). CR-1 over-expression significantly increased in vivo xenograft tumor growth of HCC cells in nude mice and in vitro HCC cell proliferation, migration, and invasion. CR-1 over-expression in the transgenic mouse livers deregulated HCC-related signaling pathways such as AKT, Wnt/ß-catenin, Stat3, MAPK/ERK, JNK, TGF-ß and Notch, as well as expression of HCC-related genes such as CD5L, S100A8, S100A9, Timd4, Orm2, Orm3, PDK4, DMBT1, G0S2, Plk2, Plk3, Gsta1 and Gsta2. However, histological signs of precancerous lesions, hepatocyte dysplasia or HCC formation were not observed in the livers of 3-, 6- or 8-month-old hepatocyte-specific CR-1-overexpressing transgenic mice. These findings demonstrate that liver-specific CR-1 overexpression in transgenic mice deregulates signaling pathways and genes associated with HCC.

14.
J Affect Disord ; 295: 264-270, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34482058

RESUMO

BACKGROUND: Major depressive disorder (MDD) is the most common mental disorder associated with suicide attempts. When a patient first visits the clinic, clinicians are often expected to make concrete diagnose about acute suicidal risk. However, the timeliness of suicide attempts correlates with patients with MDD has not been tested. METHODS: We divided 1718 first-episode and untreated MDD outpatients into those who did not have suicide attempts (non-attempts), recent suicide attempters (≤14 days before assessment) and long - dated suicide attempters (> 30 days before assessment). Positive Symptom Scale of Positive and Negative Syndrome Scale (PANSS), the 17-item Hamilton Depression Scale, 14 - item Hamilton Anxiety Scale, and clinical global impression of severity scale (CGI-S) was assessed. Body mass index, some glycolipid metabolism and thyroid hormone parameters were measured. A gradient-boosted decision trees statistical model was used to generate equally weighted classification for distinguishing recent and long - dated suicide attempters from non-attempts. RESULTS: The classifier identified higher excitement, hostility, anxiety, depression symptoms and higher free thyroxine (FT4) as risk factors for recent suicide attempters with an estimated accuracy of 87% (sensitivity, 59.1%; specificity, 61.2 %). For long - dated suicide attempters' risk factors, single status, higher anxiety and hostility symptoms, higher LDLC and lower BMI, the estimated accuracy was 88% (sensitivity, 52.8%; specificity, 49.6%). CONCLUSIONS: Risk factors for suicide attempt among patients with MDD can be identified by integrating demographic, clinical, and biological variables as early as possible during the first time see a doctor.


Assuntos
Transtorno Depressivo Maior , Tentativa de Suicídio , Ansiedade , Estudos Transversais , Transtorno Depressivo Maior/diagnóstico , Transtorno Depressivo Maior/epidemiologia , Humanos , Fatores de Risco
15.
Aging (Albany NY) ; 13(17): 21497-21512, 2021 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-34491904

RESUMO

Esophageal squamous cell carcinoma (ESCC) is a highly malignant gastrointestinal cancer with a high recurrence rate and poor prognosis. Although N6-methyladenosine (m6A), the most abundant epitranscriptomic modification of mRNAs, has been implicated in several cancers, little is known about its participation in ESCC progression. We found reduced expression of ALKBH5, an m6A demethylase, in ESCC tissue specimens with a more pronounced effect in T3-T4, N1-N3, clinical stages III-IV, and histological grade III tumors, suggesting its involvement in advanced stages of ESCC. Exogenous expression of ALKBH5 inhibited the in vitro proliferation of ESCC cells, whereas depletion of endogenous ALKBH5 markedly enhanced ESCC cell proliferation in vitro. This suggests ALKBH5 exerts anti-proliferative effects on ESCC growth. Furthermore, ALKBH5 overexpression suppressed tumor growth of Eca-109 cells in nude mice; conversely, depletion of endogenous ALKBH5 accelerated tumor growth of TE-13 cells in vivo. The growth-inhibitory effects of ALKBH5 overexpression are partly attributed to a G1-phase arrest. In addition, ALKBH5 overexpression reduced the in vitro migration and invasion of ESCC cells. Altogether, our findings demonstrate that the loss of ALKBH5 expression contributes to ESCC malignancy.

16.
Front Aging Neurosci ; 13: 718959, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34421579

RESUMO

Background: National Institute on Aging-Alzheimer's Association (NIA-AA) proposed the AT(N) system based on ß-amyloid deposition, pathologic tau, and neurodegeneration, which considered the definition of Alzheimer's disease (AD) as a biological construct. However, the associations between different AT(N) combinations and cognitive progression have been poorly explored systematically. The aim of this study is to compare different AT(N) combinations using recognized biomarkers within the Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort. Methods: A total of 341 participants were classified into cognitively unimpaired (CU; n = 200) and cognitively impaired (CI; n = 141) groups according to the clinical manifestations and neuropsychological tests. Cerebrospinal fluid (CSF) Aß42 and amyloid-PET ([18F]flutemetamol) were used as biomarkers for A; CSF phosphorylated tau (p-tau) and tau-PET ([18F]flortaucipir) were used as biomarkers for T; CSF total tau (t-tau), hippocampal volume, temporal cortical thickness, [18F]fluorodeoxyglucose (FDG) PET, and plasma neurofilament light (NfL) were used as biomarkers for (N). Binary biomarkers were obtained from the Youden index and publicly available cutoffs. Prevalence of AT(N) categories was compared between different biomarkers within the group using related independent sample non-parametric test. The relationship between AT(N) combinations and 12-year longitudinal cognition was assessed using linear mixed-effects modeling. Results: Among the CU participants, A-T-(N)- was most common. More T+ were detected using p-tau than tau PET (p < 0.05), and more (N)+ were observed using fluid biomarkers (p < 0.001). A+T+(N)+ was more common in the CI group. Tau PET combined with cortical thickness best predicted cognitive changes in the CI group and MRI predicted changes in the CU group. Conclusions: These findings suggest that optimal AT(N) combinations to determine longitudinal cognition differ by cognitive status. Different biomarkers within a specific component for defining AT(N) cannot be used identically. Furthermore, different strategies for discontinuous biomarkers will be an important area for future studies.

17.
Front Med (Lausanne) ; 8: 696374, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34368193

RESUMO

Objective: To investigate the difference of clinical features and outcomes between EM patients with and without AM after following up for at least 6 years after surgery. Methods: We retrospectively analyzed 358 EM patients who had a minimum of 6 years follow-up after laparoscopic cystectomy, which was performed by one single doctor at Peking Union Medical College Hospital from January 2009 to April 2013. All women were divided into AM group and non-AM group and analysis was performed in preoperative characteristics, surgical findings and postoperative outcomes during follow-up. Results: A total of 358 EM patients were recruited, of which 142 (39.7%) were in the AM group and the rest 216 (60.3%) in the non-AM group. Between the two group, the mean age was 34.6 vs. 32.2 years (P < 0.001). The mean operating time in the AM and non-AM group was 73.2 vs. 61.9 min (P < 0.001). According to the revised AFS classification, the mean score of the two group were 60.3 vs. 45.5 (P < 0.001). At the end of the follow-up, though the AM group was with higher rate of disease relapse, yet no significant difference was found between the two groups in statistical comparison (34/142 [23.9%] vs. 34/216 [15.7%], P = 0.053). With a minimum follow-up of 6 years after laparoscopic cystectomy, failed and successful pregnancy were seen in 107/142(75.4%) and 35/142 (24.6%) patients in the AM group vs. 114/216(52.8%) and 102/216 (47.2%) patients in the non-AM group (P < 0.05). As for the successfully pregnant patients, live births, including spontaneous pregnancy and IVF-ET, were seen in 34/35 (97.1) vs. 99/102 (97.1) patients between AM and non-AM groups, while others ended in spontaneous abortion. No significant associations were found between the two groups in infertility, leiomyoma presence, the size of ovarian endometrioma, type of deep infiltrating endometriosis (DIE) or type of recurrence (P > 0.05). Conclusion: Compared with non-AM group, EM patients with concurrent AM may have higher age, longer mean operating time and higher mean AFS score. In terms of fertility outcomes, patients in the AM group were with lower likelihood of pregnancy after surgery during the long-time follow-up.

18.
Environ Int ; 157: 106827, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34418849

RESUMO

Electrochemical oxidation (ECO) is an appealing technology for treating emerging organic pollutants in wastewater. However, the conventional flow-by ECO process is expensive with a low energy efficiency owing to the limitations of mass transport of contaminants to the limited surface area of the anode. In this study, a novel freestanding porous and permeable SnO2-Sb anode was fabricated by one-step sintering using micrometer-sized (NH4)2CO3 grains as the pore-forming agents. This permeable SnO2-Sb anode without Ti substrate functioned as a reactive anodic filter (RAF) in an ECO cell to treat wastewater containing ciprofloxacin (CIP). Forcing the wastewater through the porous RAF depth-wise improved the mass transport and vastly enlarged the electroactive surface area. Compared with the conventional flow-by configuration, the flow-through RAF exhibited a 12-fold increase in the mass transfer rate constant (60.7 × 10-6 m s-1) and a 5-fold increase in the CIP degradation rate constant (0.077 min-1). At a cell potential of 4.0 V, more than 92% of the CIP was degraded in a single-pass operation at a filtration flux of 54 L m-2 h-1 and a short retention time of 1.7 min through the RAF. The robustness and stability of the RAF were demonstrated by its remarkable CIP degradation efficacy of 99% during 200 h of operation. The mechanism of CIP degradation was examined using probe molecules and density functional theory calculations and found to be a combined effect of direct electron transfer and oxidation by generated radicals (OH and SO4-). The great potential of RAF in flow-through ECO was further validated by its effective removal (>92%) of various organic pollutants in actual municipal wastewater at a low energy consumption of 0.33 kWh m-3. The RAF-based ECO process thus provides an advanced environmental technology for the oxidation of toxic and recalcitrant organic pollutants in wastewater treatment.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Antibacterianos , Eletrodos , Oxirredução , Titânio , Águas Residuárias , Poluentes Químicos da Água/análise
19.
Sci Total Environ ; 791: 148314, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34412408

RESUMO

Integrated fixed-film activated sludge (IFAS) reactors are suitable for partial nitritation-anammox (PNA) for autotrophic nitrogen removal; however, its start-up and biofilm formation are slow and difficult. In this study, a new sludge seeding strategy was developed for the start-up of PNA-IFAS by using the pre-cultivated anammox biofilms. Two bioreactors were used in the experimental study, including a reactor that was started conventionally with the pre-acclimated suspended PNA sludge and bare biocarriers (PA-S) and a reactor that used the new seeding method with anammox biofilms pre-acclimated on biocarriers and ammonia-oxidizing bacteria (AOB) sludge in the suspension (PA-B). The use of anammox biofilms as the seed biomass greatly shortened the start-up period of the PNA-IFAS reactor to 1 month or so. Moreover, reactor PA-B achieved a higher nitrogen removal rate (707.3 mg N/(L·d)), better nitrogen removal efficiency (86.8 ± 2.8%), and lower nitrate yield (9.4%) than reactor PA-S. The biofilm development in PA-B was accelerated and its biofilm content was nearly 10 times higher than that of PA-S. The initial segregation of anammox in the biofilm and AOB in the suspended sludge provided an environment that not only accelerated the start-up of PNA-IFAS but also helped suppress the enrichment of unwanted nitrite-oxidizing bacteria (NOB) in the bioreactor, as evidenced by the lower NOB abundance in PA-B (<0.5%) than in PA-S (>2.2%) according to microbial community analysis.


Assuntos
Compostos de Amônio , Esgotos , Biofilmes , Desnitrificação , Nitrogênio
20.
J Psychiatr Res ; 141: 333-338, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34304037

RESUMO

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease involving motor, cognitive and psychiatric disturbances. HD patients with psychosis symptoms usually have bad prognosis. It is of great significance to explore the clinical, imaging and biological characteristics of HD patients with psychosis. A total of 118 Han Chinese patients with HD confirmed by Huntingtin genetic testing were recruited during 2013-2020. They were assessed by Unified Huntington's Disease Rating Scale (UHDRS) and followed up in an average of 34 months by telephone or clinical visits. Psychosis was determined by the presence of delusions or hallucinations using UHDRS-Problem Behavior Assessment. Data of magnetic resonance imaging (n = 28) and serum neurofilament light chain (NfL, n = 28) were collected in some patients. Among 118 patients (mean age 46.0 years, SD 12.0; female 53.5%), the frequency of psychosis was 14.4% (n = 17) in the cross-sectional analysis and 17.8% (n = 21) in the longitudinal observation. Probands with psychosis were predominantly female (82.3%). They exhibited worse motor, cognitive, behavioral and functional performances compared with patients without psychosis. Furthermore, the lateral ventricle volume was larger in patients with psychosis compared with patients without psychosis (p = 0.0013) while there was no difference in NfL levels between two groups. NfL levels of patients with psychosis were negatively correlated with caudate volumes (r = -0.54, p = 0.044) and white matter volumes (r = -0.57, p = 0.035). In summary, HD patients with psychosis had distinct clinical, imaging and biological features. These features might help clinicians to identify psychosis in HD patients early and provide protective interventions before adverse outcomes occur.


Assuntos
Doença de Huntington , Doenças Neurodegenerativas , Transtornos Psicóticos , China , Estudos Transversais , Progressão da Doença , Feminino , Humanos , Doença de Huntington/complicações , Doença de Huntington/diagnóstico por imagem , Doença de Huntington/genética , Pessoa de Meia-Idade , Transtornos Psicóticos/diagnóstico por imagem
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