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1.
Environ Sci Technol ; 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36099323

RESUMO

Elevated levels of reactive oxygen radicals caused by environmental stress are the key triggers of inflammation, aging, and disease; thus, it is critical to develop novel reactive oxygen radical scavenging methods with high efficiency and low toxicity. As a result of their selective reactive oxygen radical removal, hydrogen molecules are strong candidates, but their application is limited by the small hydrogen supply and short duration of action. In this study, we for the first time combined nanobubble (NB) technology and hydrogen water to remove reactive oxygen species (ROS) using copper ions as a representative environmental pollutant and Tetrahymena thermophila as a model organism. Hydrogen NBs displayed a remarkable capability of removing H2O2 and O2•- at molar ratios of 8:1 and 240:1, respectively, which were unable to be removed by dissolved hydrogen molecules only. During the oxidative defense phase, hydrogen NB water either directly removed ROS or increased the activity and relative expression of glutathione peroxidase (GSH-Px). During the oxidative inhibition phase, hydrogen NB water exerted antioxidant effects mainly by increasing the activities of superoxide dismutase and GSH-Px as well as the expression of the corresponding genes. Our results provide an important theoretical support for the wide application of hydrogen NBs in empowering the antioxidant defense system.

2.
Int J Mol Sci ; 23(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36142286

RESUMO

We previously found that cordycepin inhibits the growth and metastasis formation of MDA-MB-231 cells through the Hedgehog pathway but has not validated this in vivo. In this study, we confirmed cordycepin's anti-triple-negative breast cancer (TNBC) effect in nude mice and documented its mechanism. We found that cordycepin reduced the volume and weight of MDA-MB-231 xenografts and affected the expression of proliferation-, apoptosis-, epithelial-mesenchymal transition-, and matrix metalloproteinase-related proteins without side effects. RNA sequencing screening, pathway enrichment, and the protein network interaction analysis revealed enriched pathways and targets mainly concentrated on the Hedgehog pathway and its core components of SHH and GLI2. This indicates that the Hedgehog pathway plays a central role in the cordycepin-mediated regulation of growth and metastasis formation in TNBC. The database analysis of the Hedgehog pathway markers (SHH, PTCH1, SMO, GLI1, and GLI2) revealed that the Hedgehog pathway is activated in breast cancer tissues, and its high expression is not conducive to a patient's survival. Finally, we verified that cordycepin effectively inhibited the Hedgehog pathway in TNBC through Western blotting and immunohistochemistry. This study found that cordycepin could regulate the growth and metastasis formation of TNBC through the Hedgehog pathway in vivo, which provides new insights for targeting and treating breast cancer.

3.
Huan Jing Ke Xue ; 43(9): 4858-4866, 2022 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-36096626

RESUMO

Soil respiration is an important process in maintaining global carbon balance. Taking the Pangquangou Nature Reserve as the research area, based on the field measurement of soil respiration (Rs) data combined with altitude (ELE), soil temperature (T), soil moisture (SWC), normalized vegetation index (NDVI), slope (slope), soil total carbon (C), total nitrogen (N), and soil bulk density (BD), we analyzed the main driving forces and interactions of Rs spatial differentiation by using the geographic detector model. The results showed that:① the spatial variation of Rs and its influencing factors in the study area was moderate. The Rs was significantly positively correlated with NDVI, T, and N (P<0.01) and negatively with ELE, slope, and SWC (P<0.01). The Rs was significantly correlated with BD(P<0.05) but not with C(P>0.05). ② The multivariate linear model composed of NDVI and T explained 64.3% of Rs spatial variation. ③ ELE, T, and NDVI were the dominant driving forces of Rs spatial differentiation in the study area, which could explain 64%, 59%, and 48% of the spatial variability. ④ The interaction of the two factors enhanced the explanatory power of Rs spatial differentiation, and the maximum interaction factors were ELE∩BD (q=0.73), and T∩slope (q=0.74), respectively. Therefore, in the process of Rs estimation, combined with topographical and environmental conditions, the interaction between multiple factors should be considered.


Assuntos
Carbono , Solo , Nitrogênio , Respiração , Temperatura
4.
Oxid Med Cell Longev ; 2022: 4932304, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36071868

RESUMO

Neural tube defect (NTD) is the most common and severe embryopathy causing embryonic malformation and even death associated with gestational diabetes mellitus (GDM). Leu-Pro-Phe (LPF) is an antioxidative tripeptide isolated from hydrolysates of corn protein. However, the biological activity of LPF in vivo and in vitro remains unclear. This study is aimed at investigating the protective effects of tripeptide LPF against NTD in the high glucose exposure condition and delineate the underlying biological mechanism. We found that LPF alleviated NTD in the high glucose-exposed chicken embryo model. In addition, DF-1 chicken embryo fibroblast was loaded with high glucose for induction of oxidative stress and abnormal O-GlcNAcylation in vitro. LPF significantly decreased accumulation of reactive oxygen species and content of malondialdehyde in DF-1 cells but increased the ratio of reduced glutathione and oxidized glutathione in chick embryo. Oxygen radical absorbance capacity results showed that LPF itself had good free radical scavenging capacity and could enhance antioxidant activity of the cell content. Mechanistic studies suggested that the resistance of LPF to oxidative damage may be related to promotion of NRF2 expression and nuclear translocation. LPF alleviated the overall O-GlcNAcylation level of cellular proteins under high glucose conditions and restored the level of Pax3 protein. Collectively, our findings indicate that LPF peptide could act as a nutritional supplement for the protection of development of embryonic neural tube affected by GDM.


Assuntos
Hiperglicemia , Defeitos do Tubo Neural , Hidrolisados de Proteína , Animais , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Embrião de Galinha , Galinhas/metabolismo , Dipeptídeos , Glucose/metabolismo , Hiperglicemia/complicações , Defeitos do Tubo Neural/etiologia , Defeitos do Tubo Neural/prevenção & controle , Hidrolisados de Proteína/farmacologia , Zea mays/química
5.
Inorg Chem ; 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36084300

RESUMO

Postsynthetic modification (PSM) of the metal-organic framework (MOF) has been demonstrated to be an effective strategy to enhance performance. In this particular work, the anion framework Mn-MOF {[Mn3O(H2O)3(HTC)]2-} (HTC6- = (5'-(3,5-dicarboxyphenyl)-[1,1':3',1″-terphenyl]-3,3″,5,5″-tetracarboxylate] was obtained, and NH2(CH3)2+ ions were filled within the pores to balance the charge. In order to release the internal pores of Mn-MOF, the trivalent Fe(III) was introduced instead of Mn(II) nodes, resulting in the porous Mn1-xFex-MOF, and the NH2(CH3)2+ ions were simultaneously deported from the pores. The content of Fe(III) in Mn1-xFex-MOF was highly dependent on the concentration of Fe(III) solution, and the maximum could be up to Mn0.05Fe0.95-MOF with a BET surface area of 1209.457 m2 g-1. Compared to the amorphization of dense Mn-MOF at 0.8 GPa in a diamond anvil cell, the mechanical stability of porous Mn0.05Fe0.95-MOF has been dramatically enhanced, and the framework integrity could be maintained up to 16.5 GPa. The proton conductivity for the Mn1-xFex-MOF series was also investigated, where Mn0.93Fe0.07-MOF showed the best performance of 1.47 × 10-2 S cm-1 under 70 °C and 98% RH due to the onset of reversed charge from the anionic framework to cationic framework and the formation of the most compact hydrogen bonding net. This work has not only provided an example for the PSM strategy but also illustrated that the versatile functionalities of MOF materials were mainly ascribed to the tunable porosity.

6.
Stem Cells ; 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36087098

RESUMO

Long noncoding RNAs (lncRNAs) emerge as important orchestrators of biological processes in embryonic stem cells (ESCs). LncRNA Lx8-SINE B2 was recently identified as an ESC-specific lncRNA which marks pluripotency. Here, we studied the function of lncRNA Lx8-SINE B2 in ESCs. Depletion of Lx8-SINE B2 disrupted ESC proliferation, repressed the expression of pluripotency genes, activated differentiation genes, and inhibited reprogramming to induced pluripotent stem cells. The reduction of the colony formation ability of ESCs upon Lx8-SINE B2 knockdown was accompanied by the elongation of G1 phase and the shortening of S phase. Transcriptome analysis revealed that Lx8-SINE B2 deficiency affected multiple metabolic pathways, particularly glycolysis. Mechanistically, Lx8-SINE B2 functions as a cytoplasmic lncRNA and interacts with the glycolytic enzyme Eno1 as shown by RNA pull-down and RNA localization analysis. Lx8-SINE B2 and Eno1 interact with and regulate each other's expression, hence promoting the expression of metabolic genes and influencing glycolysis. In conclusion, we have identified lncRNA Lx8-SINE B2 as a novel regulator of ESC proliferation, cell cycle, and metabolism through working with Eno1.

7.
Quant Imaging Med Surg ; 12(9): 4647-4657, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36060582

RESUMO

Background: Computed tomography (CT) imaging is the most important and common means of detecting and diagnosing pelvic bone tumors. While phantoms with sufficient flexibility and anatomical realism are useful in CT research, using phantoms has been difficult for pelvic bone tumors because of the tumors' relatively large size and highly variable shape. By combining medical 3D printing technology and fresh tumor specimens, this study aimed to design such a hybrid phantom, test its imaging properties, and demonstrate its usefulness in optimizing the CT protocols. Methods: Two phantoms were designed for 2 patients with pelvic bone tumors who underwent surgical resection. One phantom was scanned with a routine pelvic CT protocol and compared against the patient image to test the imaging properties. We optimized the imaging protocol by assessing a series of varied settings on tube voltage (80, 100, 120, and 140 kVp), tube current (80, 120, and 160 to 200 mAs), and pitch factor (0.5, 0.8, 1.1, and 1.4) using the other phantom. These were assessed in comparison to the clinical reference of 140 kVp, 240 mAs, and 1.0 pitch, respectively. Image quality was quantified in terms of CT value, image noise, signal to noise ratio (SNR), and contrast to noise ratio (CNR) in various regions of interest. Results: With the routine protocol, the phantom image showed no significant difference in CT values of the bone and soft tissues and image noise compared to the patient image (all P values >0.05). With a lower tube voltage (80, 100, and 120 kVp) than the reference protocol, the CT value of bone tissue showed significant differences (all P values <0.001). No significant difference was found when applying a reduced tube current (all P values >0.05). With an increased helical pitch, pitches of 0.5, 0.8 and 1.1 were found to be comparable to those using the reference protocol (all P values >0.05). Conclusions: The 3D-printed phantom can simulate the radiological properties of tumors in the pelvis and was successfully used in imaging studies of pelvic bone tumors. According to our preliminary findings, a low-dose pelvic CT protocol with acceptable image quality is achievable using reduced tube current or increased pitch.

8.
Polymers (Basel) ; 14(17)2022 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-36080603

RESUMO

In order to solve the problem of low electrical conductivity of carbon nanofiber membranes, a novel triple crosslinking strategy, including pre-rolling, solvent and chemical imidization crosslinking, was proposed to prepare carbon nanofiber membranes with a chemical crosslinking structure (CNMs-CC) derived from electrospinning polyimide nanofiber membranes. The physical-chemical characteristics of CNMs-CC as freestanding anodes for lithium-ion batteries were investigated in detail, along with carbon nanofiber membranes without a crosslinking structure (CNMs) and carbon nanofiber membranes with a physical crosslinking structure (CNMs-PC) as references. Further investigation demonstrates that CNMs-CC exhibits excellent rate performance and long cycle stability, compared with CNMs and CNMs-PC. At 50 mA g-1, CNMs-CC delivers a reversible specific capacity of 495 mAh g-1. In particular, the specific capacity of CNMs-CC is still as high as 290.87 mAh g-1 and maintains 201.38 mAh g-1 after 1000 cycles at a high current density of 1 A g-1. The excellent electrochemical performance of the CNMs-CC is attributed to the unique crosslinking structure derived from the novel triple crosslinking strategy, which imparts fast electron transfer and ion diffusion kinetics, as well as a stable structure that withstands repeated impacts of ions during charging and discharging process. Therefore, CNMs-CC shows great potential to be the freestanding electrodes applied in the field of flexible lithium-ion batteries and supercapacitors owing to the optimized structure strategy and improved properties.

9.
Microbiol Resour Announc ; : e0071022, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094210

RESUMO

Here, we announce the draft genome sequence of Lactobacillus brevis 2-34, a strain isolated from the fermentation process of Shaoxing huangjiu (Chinese rice wine). The genome size of 2-34 was 2,557,496 bp, with 2,459 coding genes, 67 tRNAs, and 2 rRNAs.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36094712

RESUMO

The development of electric vehicles (EVs) is not only a necessary path for China to move from a big country to a powerful country in automobile industry, but also a strategic measure to achieve the goal of "peak carbon dioxide emissions" and "carbon neutrality." However, in the low and high temperature environment, EVs may face problems such as a large reduction in cruising range, slow charging and poor safety, which decrease the attractiveness of EVs to consumers and hinder the EV adoption. However, existing research rarely pays attention to the impact of temperature on the EV adoption. Based on the panel data of electric vehicle sales in 20 Chinese provinces from 2010 to 2018, this paper uses interaction fixed effect (FE) model to test the effect of temperature on the EV adoption and explains the causes of regional differences in EV adoption. The study has the following findings. Firstly, EV sales show an inverted U-shaped trend with the change of temperature. Secondly, compared to extreme high temperature, extreme low temperature has a greater negative impact on the EV adoption. Thirdly, the negative impact of extreme temperature on battery electric vehicles (BEVs) is greater than that of plug-in hybrid electric vehicles (PHEVs). Finally, temperature will make consumers' behavior adaptive by affecting their expectations. These findings can provide policy makers, department of urban planning and building, EV technology and quality inspection department, non-governmental organizations, and EV manufacturer, aiming at accelerating the market proliferation of EVs, with theoretical basis and targeted insights.

11.
Front Psychol ; 13: 883352, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35992422

RESUMO

Emerging adult newlywed couples often experience many demands on their time, and three common problems may surface as couples try to balance these demands-problems related to finances, sleep, and sex. We used two waves of dyadic data from 1,001 emerging adult newlywed couples to identify four dyadic latent profiles from husbands' and wives' financial management behaviors, sexual satisfaction, and sleep quality: Flounderers, Financially Challenged Lovers, Drowsy Budgeters, and Flourishers. We then examined how husbands' and wives' marital satisfaction, in relation to profile membership, varied at a later wave. We found that Financially Challenged Lovers and Flourishers had significantly higher marital satisfaction than Drowsy Budgeters and Flounderers (mostly medium effect sizes). Whereas, Financially Challenged Lovers and Flourishers did not differ in terms of marital satisfaction, Drowsy Budgeters seemed to have slightly higher marital satisfaction than Flounderers for wives only (small effect size). However, we did not find evidence that these connections meaningfully differed by sex. Implications for the efforts of clinicians and educators are discussed.

12.
Anticancer Drugs ; 33(9): 975-978, 2022 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-35946538

RESUMO

The reason that immune checkpoint inhibitors have not been widely applied to pancreatic cancer treatment is probably because of low immunogenicity or dense stromal fibrosis. Recently, only pembrolizumab was recommended for DNA mismatch repair deficiency or high microsatellite instability by National Comprehensive Cancer Network guideline. Pancreatic ductal adenocarcinoma (PDAC) accounts for more than 90% of pancreatic cancer, with a poor overall survival rate, the value of immunotherapy for PDAC needs more research. Here, we report a 56-year-old man suffered from PDAC with liver metastasis after radical surgery. The next-generation sequencing result demonstrated that it had remarkably high tumor mutational burden (TMB) of 49.92 Muts/Mb and microsatellite stability. Sintilimab (anti-PD-1) monotherapy was continuously administrated after failure of combined chemotherapy in second line, achieving stable disease within 22 months and few immunotherapy-related adverse events. To our knowledge, this is the first time to report a good outcome achieving 22 months with progression-free survival after PDAC metastasis with monotherapy of sintilimab. TMB may serve as a potential efficacy-related predictor in PDAC patients with sintilimab and help physicians make optimum clinical strategy.

13.
Chemosphere ; 307(Pt 2): 135805, 2022 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-35917979

RESUMO

Seawater intrusion can cause environmental risks to paddy soils around estuaries, but the impacts on the availability of heavy metals are still unclear. River water and sea water were collected along the river of an estuary. A stirred-flow experiment was conducted to examine the Cd desorption behavior in Cd-contaminated paddy soil. While the pH increased with increasing salinity levels, more Cd was released with increasing salinity, suggesting that Cd competition by cations and complexation by anions, but not pH, dominated the release of Cd from soils. Moreover, paddy soil was incubated at different salinities under alternating redox conditions. The availability of Cd, as indicated by the diffusive gradients in thin film (DGT), became relatively high with increasing salinity levels during the initial anaerobic and later aerobic stages. The available Cd fractions substantially decreased under anaerobic condition, and then rapidly increased under aerobic condition. When oxygen was introduced into the system, Cd associated with organic matter and Fe-Mn oxides were released, and oxidative dissolution of Cd sulfides was observed, especially in the high salinity treatment. Seawater intrusion affects biogeochemical cycles and can promote rapid export of NH4+, Fe2+, and SO42- in paddy soils, especially in soils with high salinity. Our findings demonstrated that the high salinity content in paddy soil significantly enhanced the availability of Cd, especially during the drainage stage.

14.
RSC Adv ; 12(34): 21904-21915, 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-36043084

RESUMO

Free-standing and flexible carbon nanofiber membranes (CNMs) with a three-dimensional network structure were fabricated based on PMDA/ODA polyimide by combining electrospinning, imidization, and carbonization strategies. The influence of carbonization temperature on the physical-chemical characteristics of CNMs was investigated in detail. The electrochemical performances of CNMs as free-standing electrodes without any binder or conducting materials for lithium-ion batteries were also discussed. Furthermore, the surface state and internal carbon structure had an important effect on the nitrogen state, electrical conductivity, and wettability of CNMs, and then further affected the electrochemical performances. The CNMs/Li metal half-cells exhibited a satisfying charge-discharge cycle performance and excellent rate performance. They showed that the reversible specific capacity of CNMs carbonized at 700 °C could reach as high as 430 mA h g-1 at 50 mA g-1, and the value of the specific capacity remained at 206 mA h g-1 after 500 cycles at a high current density of 1 A g-1. Overall, the newly developed carbon nanofiber membranes will be a promising candidate for flexible electrodes used in high-power lithium-ion batteries, supercapacitors and sodium-ion batteries.

15.
Molecules ; 27(16)2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-36014323

RESUMO

Glaesserella parasuis (GPS), a causative agent of Glässer's disease, is thought to be the main fatal cause of peritonitis in swine, thus resulting in high mortality and morbidity and significant economic losses to the swine industry. However, the mechanisms of GPS infection-induced apoptosis and possible therapeutic pathway for GPS infection in peritonitis remain unclear. Baicalin has important biological functions during disease treatment, such as antiviral, bacterial inhibition, anti-apoptosis, and anti-inflammatory. However, whether baicalin has anti-apoptotic effects during the process of GPS infection in peritonitis is unclear. In the present study, the anti-apoptotic effect and mechanisms of baicalin in GPS infection-induced apoptosis were investigated in porcine peritoneal mesothelial cells (PPMC). The results showed that baicalin could inhibit the apoptosis rate occurrence of PPMC induced by GPS to various degrees and inhibit the expression of apoptosis-related genes and cleaved caspase-3. Meanwhile, baicalin significantly antagonized the expression of p-JNK, p-p38, and p-ERK induced by GPS in PPMC. These findings for the first time demonstrate that baicalin exerted the effect of antagonizing GPS induced apoptosis in PPMC by inhibiting the activation of the PKC-MAPK pathway and could be a therapeutic option in the management of GPS infection.


Assuntos
Haemophilus parasuis , Peritonite , Doenças dos Suínos , Animais , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Peritonite/tratamento farmacológico , Suínos , Doenças dos Suínos/tratamento farmacológico
16.
Front Immunol ; 13: 927880, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35911686

RESUMO

Heavy-metal pollution has increasingly jeopardized the habitats of marine organisms including the sea cucumber, a seafloor scavenger vital to seawater bio-decontamination, ocean de-acidification and coral-reef protection. Normal physiology including immune functions of sea cucumbers is toxicologically modulated by marine metal pollutants such as cadmium (Cd). The processes underpinning Cd's toxic effects on immune systems in the sea cucumber, Holothuria leucospilota, are still poorly understood. To this end, we cloned and characterized a full-length caspase-9 (Hl-CASP9) cDNA in the sea cucumber, Holothuria leucospilota. Hl-CASP9 mRNA levels evolved dynamically during embryonic development. Coelomocytes, a type of phagocytic immune effectors central to H. leucospilota immunity, were found to express Hl-CASP9 mRNA most abundantly. Hl-CASP9 protein structurally resembles caspases-2 and -9 in both invertebrate and vertebrate species, comprising a CARD domain and a CASc domain. Remarkably, Hl-CASP9 was transcriptionally sensitive to abiotic oxidative stress inducers including hydrogen peroxide (H2O2), nitric oxide (•NO) and cadmium (Cd), but insensitive to immunostimulants including lipopolysaccharide (LPS), and poly(I:C). Overexpression of Hl-CASP9 augmented mitochondria-dependent apoptosis in HEK293T cells, while knock-down of Hl-CASP9 blunted Cd-induced coelomocyte apoptosis in vivo. Overall, we illustrate that an evolutionarily ancient caspase-9-dependent pathway exists to sensitize coelomocytes to premature cell death precipitated by heavy metal pollutants, with important implications for negative modulation of organismal immune response in marine invertebrates.


Assuntos
Apoptose , Cádmio , Caspase 9 , Holothuria , Animais , Apoptose/genética , Cádmio/toxicidade , Caspase 9/metabolismo , Poluentes Ambientais , Células HEK293 , Holothuria/genética , Holothuria/metabolismo , Humanos , Peróxido de Hidrogênio , RNA Mensageiro/genética , Pepinos-do-Mar/genética , Pepinos-do-Mar/metabolismo
17.
Transl Cancer Res ; 11(7): 2338-2347, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35966305

RESUMO

Background: A non-invasive, simple, and convenient method to evaluate the presence of epidermal growth factor receptor (EGFR) mutations is important for initial treatment decisions in lung adenocarcinoma. Methods: We retrospectively reviewed 297 untreated primary lung adenocarcinoma patients with exact EGFR status. Based on their EGFR status, the patients were divided into a mutant-type group (138 patients) and wild-type group (159 patients). General patient characteristics and possible factors reflecting the status of EGFR were also evaluated. Results: Of the 297 lung adenocarcinoma patients analyzed for EGFR status who underwent positron emission tomography (PET)/computed tomography (CT) between January 2013 and December 2017, mutations in the EGFR gene were detected in 138 patients (46.5%). EGFR mutations were more frequently associated with women, never smokers, and low 18F-fluoro-2-deoxy-glucose (18F-FDG) PET/CT maximal standard uptake value of the primary tumor (pSUVmax). Multivariate analysis indicated that women [odds ratio (OR) =2.853; 95% confidence interval (CI): 1.451-5.611; P=0.002], never smokers (OR =2.414; 95% CI: 1.217-4.789; P=0.012), tumor size <3.5 cm (OR, 2.170; 95% CI: 1.205-3.908; P=0.010), and pSUVmax <8.2 (OR =1.904; 95% CI: 1.098-3.302; P=0.022) were effective predictors of EGFR mutation. In addition, the area under the curve (AUC) of pSUVmax and tumor size was 0.623 and 0.600, respectively. Combined with clinical characteristics, including sex and smoking status, the AUC of the 4 predictors was 0.770. Conclusions: These indicators could be helpful for enhancing predictive accuracy of EGFR mutations in lung adenocarcinoma patients, especially in those for whom EGFR detection is unavailable.

18.
Sci Total Environ ; 851(Pt 1): 158068, 2022 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-35987227

RESUMO

Microbially mediated Fe(II) oxidation is prevalent and thought to be central to many biogeochemical processes in paddy soils. However, we have limited insights into the Fe(II) oxidation process in paddy fields, considered the world's largest engineered wetland, where microoxic conditions are ubiquitous. In this study, microaerophilic Fe(II) oxidizing bacteria (FeOB) from paddy soil were enriched in gradient tubes with FeS, FeCO3, and Fe3(PO4)2 as iron sources to investigate their capacity for Fe(II) oxidation and carbon assimilation. Results showed that the highest rate of Fe(II) oxidation (k = 0.836 mM d-1) was obtained in the FeCO3 tubes, and cells grown in the Fe3(PO4)2 tubes yielded maximum assimilation amounts of 13C-NaHCO3 of 1.74% on Day 15. Amorphous Fe(III) oxides were found in all the cell bands with iron substrates as a result of microbial Fe(II) oxidation. Metagenomics analysis of the enriched microbes targeted genes encoding iron oxidase Cyc2, oxygen-reducing terminal oxidase, and ribulose-bisphosphate carboxylase, with results indicated that the potential Fe(II) oxidizers include nitrate-reducing FeOB (Dechloromonas and Thiobacillus), Curvibacter, and Magnetospirillum. By combining cultivation-dependent and metagenomic approaches, our results found a number of FeOB from paddy soil under microoxic conditions, which provide insight into the complex biogeochemical interactions of iron and carbon within paddy fields. The contribution of the FeOB to the element cycling in rice-growing regions deserves further investigation.

19.
Nanoscale ; 14(34): 12297-12312, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-35960150

RESUMO

In the present study, we investigate the combined interaction of mesoporous silica (SiO2) and photocatalytic titanium dioxide (TiO2) nanoparticles with lipid membranes, using neutron reflectometry (NR), cryo-transmission electron microscopy (cryo-TEM), fluorescence oxidation assays, dynamic light scattering (DLS), and ζ-potential measurements. Based on DLS, TiO2 nanoparticles were found to display strongly improved colloidal stability at physiological pH of skin (pH 5.4) after incorporation into either smooth or spiky ("virus-like") mesoporous silica nanoparticles at low pH, the latter demonstrated by cryo-TEM. At the same time, such matrix-bound TiO2 nanoparticles retain their ability to destabilize anionic bacteria-mimicking lipid membranes under UV-illumination. Quenching experiments indicated both hydroxyl and superoxide radicals to contribute to this, while NR showed that free TiO2 nanoparticles and TiO2 loaded into mesoporous silica nanoparticles induced comparable effects on supported lipid membranes, including membrane thinning, lipid removal, and formation of a partially disordered outer membrane leaflet. By comparing effects for smooth and virus-like mesoporous nanoparticles as matrices for TiO2 nanoparticles, the interplay between photocatalytic and direct membrane binding effects were elucidated. Taken together, the study outlines how photocatalytic nanoparticles can be readily incorporated into mesoporous silica nanoparticles for increased colloidal stability and yet retain most of their capacity for photocatalytic destabilization of lipid membranes, and with maintained mechanisms for oxidative membrane destabilization. As such, the study provides new mechanistic information to the widely employed, but poorly understood, practice of loading photocatalytic nanomaterials onto/into matrix materials for increased performance.


Assuntos
Nanopartículas , Dióxido de Silício , Catálise , Lipídeos , Titânio
20.
Nephron ; : 1-6, 2022 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-35901785

RESUMO

CONTEXT: Patients with membranous nephropathy (MN) are recognized as individuals with high risk of thrombosis. However, prophylactic anticoagulant therapy in this population is still a controversial topic for a lack of high-quality evidence. Subject of Review: The Kidney Disease: Improving Global Outcomes (KDIGO) 2021 Clinical Practice Guideline for the Management of Glomerular Diseases was published in Kidney International in October 2021, and it was updated on the topic of prophylactic anticoagulant therapy in patients with MN. Differing from the previous main concern about the risk of venous thromboembolism (VTE) in MN, it paid attention to the risk of arterial thromboembolism (ATE) as well. Additionally, the risk of ATE was considered to be associated with hypoalbuminemia. A tool for evaluating the risk of bleeding in patients with MN was proposed in the KDIGO 2021 guideline, and individuals with low risk of bleeding as well as high risk of VTE were suggested to use warfarin or low-molecular-weight heparin (LMWH) combined with aspirin, as an alternative regimen for warfarin. Second Opinion: Our analysis shows that no consensuses have been reached on whether the prevention of ATE is necessary for patients with MN or whether the risk of ATE is associated with hypoalbuminemia. The proposed tool is not the only choice of tools for bleeding assessment, and the HAS-BLED risk score might be a better choice from the perspective of general applicability and availability. Furthermore, in our opinion, the suggestion for prophylaxis regimen of LMWH combined with aspirin showed a lack of consideration and might be inappropriate to some degree. In summary, there are still many controversies in the field of prophylactic anticoagulation for MN; as a consequence, more high-quality studies are required to provide guidance.

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