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1.
Ann Dyslexia ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33615418

RESUMO

The influence of test anxiety on academic difficulties has been investigated in typically developing students, but the mechanism underlying the influence remains unclear, especially for those with specific learning disabilities (SpLDs). This study examined the role of metacognition in the relationship between test anxiety and literacy difficulties among Chinese typically developing adolescents as well as those who have been identified as having an SpLD and significant literacy difficulties (i.e., typically functioning SpLD) and without significant literacy difficulties (i.e., high-functioning SpLD) in Taiwan. A total of 238 first-year undergraduate students were recruited from eleven universities in South Taiwan. Among 238 students, 105 were identified to have SpLDs, and 133 were typically developing students. These students were asked to complete questionnaires on demographics, test anxiety, metacognition, and literacy difficulties (i.e., reading and writing). Structural equation modeling analyses showed that test anxiety among Chinese adolescents was linked to literacy difficulties but that only high-functioning and typically functioning students with SpLDs experienced a direct effect (without mediation by other factors). For those without SpLDs, the influence of test anxiety on literacy difficulties was not direct but significantly mediated by metacognition. Various components of these students' metacognition had mediating effects on different literacy difficulties. Test anxiety might influence the reading and writing difficulties of typically developing adolescents and those with typically functioning and high-functioning SpLDs through different mechanisms. Moreover, teachers at the university level are encouraged to consider students' test anxiety and metacognition when preparing their teaching materials.

2.
J Agric Food Chem ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33617251

RESUMO

In this study, modified whey protein hydrolysates (WPH) were obtained after succinic anhydride succinylation and linear dextrin glycation, and emulsion gels were prepared on the basis of unmodified/modified WPH stabilized emulsions with sugar beet pectin (SBP) addition and laccase-catalyzed cross-linking. The influences of emulsifier types and SBP contents on the texture of emulsion gels were estimated. The texture and rheological properties of emulsion gels were characterized. An ideal gel emulsion was formed when the SBP content was 3% (w/w). A uniform network was observed in emulsion gels stabilized by W-L, W-L-S, and W-S-L. In addition, the effect of the emulsifier type on the bioaccessibility of curcumin encapsulated in emulsion gels was investigated and the W-S-L stabilized emulsion gel exhibited the highest curcumin bioaccessibility (65.57%). This study provides a theoretical basis for the development of emulsion gels with different textures by SBP addition and laccase cross-linking as encapsulation delivery systems.

3.
Med Image Anal ; 69: 101954, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33550006

RESUMO

Limb salvage surgery of malignant pelvic tumors is the most challenging procedure in musculoskeletal oncology due to the complex anatomy of the pelvic bones and soft tissues. It is crucial to accurately resect the pelvic tumors with appropriate margins in this procedure. However, there is still a lack of efficient and repetitive image planning methods for tumor identification and segmentation in many hospitals. In this paper, we present a novel deep learning-based method to accurately segment pelvic bone tumors in MRI. Our method uses a multi-view fusion network to extract pseudo-3D information from two scans in different directions and improves the feature representation by learning a relational context. In this way, it can fully utilize spatial information in thick MRI scans and reduce over-fitting when learning from a small dataset. Our proposed method was evaluated on two independent datasets collected from 90 and 15 patients, respectively. The segmentation accuracy of our method was superior to several comparing methods and comparable to the expert annotation, while the average time consumed decreased about 100 times from 1820.3 seconds to 19.2 seconds. In addition, we incorporate our method into an efficient workflow to improve the surgical planning process. Our workflow took only 15 minutes to complete surgical planning in a phantom study, which is a dramatic acceleration compared with the 2-day time span in a traditional workflow.

4.
Environ Sci Technol ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33600162

RESUMO

Electrotrophs play an important role in biogeochemical cycles, but the effects of long-term fertilization on electrotrophic communities in paddy soils remain unclear. Here, we explored the responses of electrotrophic communities in paddy soil-based microcosms to different long-term fertilization practices using microbial electrosynthesis systems (MESs), high-throughput quantitative PCR, and 16s rRNA gene-based Illumina sequencing techniques. Compared to the case in the unfertilized soil (CK), applications of only manure (M); only chemical nitrogen, phosphorous, and potassium fertilizers (NPK); and M plus NPK (MNPK) clearly changed the electrotrophic bacterial community structure. The Streptomyces genus of the Actinobacteria phylum was the dominant electrotroph in the CK, M, and MNPK soils. The latter two soils also favored Truepera of Deinococcus-Thermus or Arenimonas and Thioalkalispira of Proteobacteria. Furthermore, Pseudomonas of Proteobacteria and Bacillus of Firmicutes were major electrotrophs in the NPK soil. These electrotrophs consumed biocathodic currents coupled with nitrate reduction and recovered 18-38% of electrons via dissimilatory nitrate reduction to ammonium (DNRA). The increased abundances of the nrfA gene for DNRA induced by electrical potential further supported that the electrotrophs enhanced DNRA for all soils. These expand our knowledge about the diversity of electrotrophs and their roles in N cycle in paddy soils and highlight the importance of fertilization in shaping electrotrophic communities.

5.
Rev Sci Instrum ; 92(1): 013704, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33514196

RESUMO

Exploring the corresponding relation between structural and physical properties of materials at the atomic scale remains the fundamental problem in science. With the development of the aberration-corrected transmission electron microscopy (AC-TEM) and the ultrafast optical spectroscopy technique, sub-angstrom-scale spatial resolution and femtosecond-scale temporal resolution can be achieved, respectively. However, the attempt to combine both their advantages is still a great challenge. Here, we develop in situ optical spectroscopy with high temporal resolution in AC-TEM by utilizing a self-designed and manufactured TEM specimen holder, which has the capacity of sub-angstrom-scale spatial resolution and femtosecond-scale temporal resolution. The key and unique design of our apparatus is the use of the fiber bundle, which enables the delivery of focused pulse beams into TEM and collection of optical response simultaneously. The generated focused spot has a size less than 2 µm and can be scanned in plane with an area larger than 75 × 75 µm2. Most importantly, the positive group-velocity dispersion caused by glass fiber is compensated by a pair of diffraction gratings, thus resulting in the generation of pulse beams with a pulse width of about 300 fs (@ 3 mW) in TEM. The in situ experiment, observing the atomic structure of CdSe/ZnS quantum dots in AC-TEM and obtaining the photoluminescence lifetime (∼4.3 ns) in the meantime, has been realized. Further ultrafast optical spectroscopy with femtosecond-scale temporal resolution could be performed in TEM by utilizing this apparatus.

6.
Sci Rep ; 11(1): 805, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33436941

RESUMO

The long noncoding RNASBF2-AS1 can promote the occurrence and development of many kinds of tumours, but its role in oesophageal squamous cell carcinoma (ESCC) is unknown. We found that SBF2-AS1 was up-regulated in ESCC, and its expression was positively correlated with tumor size (P = 0.0001), but was not related to gender, age, TNM stage, histological grade, and lymphnode metastasis (P > 0.05). It was further found that the higher the expression of SBF2-AS1, the lower the survival rate. COX multivariate analysis showed that the expression of SBF2-AS1 was an independent prognostic factor. Functional experiments show that inhibition of SBF2-AS1 can inhibit the proliferation of ESCC through in vivo and in vitro, and overexpression of SBF2-AS1 can promote the proliferation of ESCC and inhibit its apoptosis. In mechanism, SBF2-AS1/miR-338-3P, miR-362-3P/E2F1 axis are involved in the regulation of ESCC growth. In general, SBF2-AS1 may be used as ceRNA to combine with miR-338-3P and miR-362-3P to up-regulate the expression ofE2F1, and ultimately play a role in promoting cancer. It may be used as a therapeutic target and a biomarker for prognosis.

7.
J Agric Food Chem ; 69(4): 1283-1290, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33464890

RESUMO

The substantial application of organophosphate triesters (tri-OPEs) may lead to a concentration escalation of their major metabolites, organophosphate diesters (di-OPEs) in animal-derived and plant-derived animal protein supplement feeds (APFs). APFs are major food for raised animals and may bring OPEs into the food supply. In the present study, the concentrations of Σ8di-OPEs in animal-derived and plant-derived APFs were in the range of 1.98-182 ng/g dw (average: 39.2 ng/g dw). Meat meal had the highest average concentrations of di-OPEs (52.1 ng/g dw), followed by blood meal (49.9 ng/g), feather meal (23.3 ng/g dw), and plant-derived feeds (18.3 ng/g dw). The concentrations of di-OPEs were at the same order of magnitude as those of tri-OPEs in APFs. Bis(1,3-dichloro-2-propyl) phosphate (BDCIPP) was the major contributor in blood meal, feather meal, and plant-derived APFs, while dimethyl phosphate dominated in meat meal. The ratios of di-OPEs/tri-OPEs (Rdi/tri) displayed large variability, ranging from 0 for the bis(2-chloroethyl) phosphate-tris(2-chloroethyl) phosphate pair to 175 for the BDCIPP-tris(1,3-dichloroisopropyl) phosphate pair, which indicated that the metabolism capacities and environmental sources for di-OPEs are diverse in APFs. Different Rdi/tri between APFs and similar food matrices implied that di-OPEs may have different environmental sources. The similar Rdi/tri values for some of the di-/tri-OPE pairs among APFs and dust samples indicated that dust may be a direct exogenous source of OPE exposure in some APF matrices. Future studies should simultaneously focus on tri- and di-OPEs, together of which may reflect the actual exposure to OPEs through the food supply.

8.
J Affect Disord ; 282: 211-218, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33418369

RESUMO

BACKGROUND: Emerging adulthood is a life stage with elevated risk for both mental disorders and financial distress. Although a positive link between financial stress and depressive symptoms has been identified, there is a lack of delineation on the temporal dynamics of this link spanning the entire stage of emerging adulthood (roughly ages 18 to 29). METHODS: Using a statistical approach that partitions between-person from within-person variation and based on four waves of data from a college cohort (N = 2,098) throughout emerging adulthood, this study addresses this gap. RESULTS: Latent growth curve model analyses indicate that the trajectory of financial stress throughout emerging adulthood followed an inverted "U" shape, whereas that of depressive symptoms displayed a linear, decreasing trend. The positive correlations of both intercepts and slopes between financial stress and depressive symptoms indicated a co-development pattern. Classical, cross-lagged panel model analyses (i.e., a model aggregating between-person and within-person variation) demonstrated a reciprocal positive association between financial stress and depressive symptoms across waves. Random intercept, cross-lagged panel model analyses (i.e., a model disaggregating between-person and within-person effects) indicated a unidirectional positive within-person effect from depressive symptoms to financial stress across waves, controlling for between-person effects. LIMITATIONS: Shared-method and shared-informant variance may inflate the identified associations, and the correlational data precludes casual inferences. CONCLUSION: Improving young adults' mental well-being, specifically intervening depressive symptoms, could be an avenue for reducing their financial stress. Future research is pressing to examine mechanisms via which depression symptoms manifest as financial stress during transition to adulthood.

9.
Neurochem Res ; 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33481205

RESUMO

S-oxiracetam (S-ORC), a nootropic drug, was used to protect against ischemic stroke by lessening the blood brain barrier dysfunction and inhibiting neuronal apoptosis. However, the potential effects of S-ORC in the recovery of cognitive functions after ischemic stroke and the underlying mechanisms remains unclear. In this study, middle cerebral artery occlusion/reperfusion (MCAO/R) in rats was used as the animal model. By using Y-maze test, Morris water maze, triphenyl tetrazolium chloride (TTC) staining, terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate (dUTp) nick end labeling (TUNEL) assay, hematoxylin and eosin, immunohistochemical staining and western blot to evaluate the protective effect of S-ORC on cognitive recovery, we were able to confirm that S-ORC ameliorated spatial learning impairment, tissue loss, and hippocampal neuronal apoptosis and injury induced by MCAO/R in rats. These cognitive effects were achieved by restoring the normal function of synaptophysin and increasing PSD95 expression in the hippocampus. Furthermore, we found that methyllycaconitine, the antagonist of α7 nicotinic acetylcholine receptor (α7nAChR), and LY294002, the inhibitor of phosphoinositide 3-kinase (PI3K), were able to block the cognitive effects of S-ORC after MCAO/R in rats. In conclusion, α7nAChR and PI3K are key molecules that mediated the signaling pathway leading to S-ORC-induced cognitive restoration after MCAO/R.

10.
J Hazard Mater ; 411: 125076, 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33485226

RESUMO

Aquatic foods are important sources of chlorinated paraffins (CPs) to humans. Farmed crabs are bottom-dwelling aquatic animals, and can accumulate CPs through food and aquatic environment. However, limited information is available on CPs concentrations in and sources to farmed crabs. In this study, short-chain CPs (SCCPs) and medium-chain CPs (MCCPs) concentrations in 59 samples from the farmed crab food web (crab, crab feed, and aquaculture environment) were determined. The samples were from 17 crab farms in Anhui Province, Jiangxi Province, and Shanghai, in China. The SCCPs and MCCPs concentrations in the crab samples were 82-1760 and not detected-680 ng/g lipid weight, respectively. The dominant SCCPs and MCCPs in the crab samples were C10Cl6-7 and C14Cl7-8, respectively. The SCCPs concentrations in the crab food web samples were higher than the MCCPs concentrations. SCCPs contamination of the crab food web samples was not directly related to CPs product use. The main factors affecting SCCPs concentrations in the crab samples were different for different crab farms. Crab feed and the aquaculture environment could be sources of SCCPs to farmed crabs. No marked health risks are posed to humans through consuming CPs in Chinese mitten crabs farmed in China.

11.
Mol Cancer ; 20(1): 14, 2021 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-33430876

RESUMO

Currently, there is no strong evidence of the well-established biomarkers for immune checkpoint inhibitors (ICIs) in nasopharyngeal carcinoma (NPC). Here, we aimed to reveal the heterogeneity of tumour microenvironment (TME) through virtual microdissection of gene expression profiles. An immune-enriched subtype was identified in 38% (43/113) of patients, which was characterized by significant enrichment of immune cells or immune responses. The remaining patients were therefore classified as a non-Immune Subtype (non-IS), which exhibited highly proliferative features. Then we identified a tumour immune evasion state within the immune-enriched subtype (18/43, 42%), in which high expression of exclusion- and dysfunction-related signatures was observed. These subgroups were designated the Evaded and Active Immune Subtype (E-IS and A-IS), respectively. We further demonstrated that A-IS predicted favourable survival and improved ICI response as compared to E-IS and non-IS. In summary, this study introduces the novel immune subtypes and demonstrates their feasibility in tailoring immunotherapeutic strategies.

12.
J Hazard Mater ; 406: 124680, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33310329

RESUMO

Integrated-remediation technologies on heavy metal polluted sediments have received much attention. In this study, Cd contaminated sediments were treated with various conditions: sulfate reducing bacteria (SRB) only and SRB combined with different dosages of nano zero valent iron (nZVI (0.5-10 mg/g)). The immobilization of Cd was found in all remediation treatments according to the decreases of mobile Cd and the increases of more stable Cd compared with control. Five typical SRBs (Desulfobulbaceae, Desulfobacteraceae, Syntrophobacteraceae, Desulfovibrionaceae and Desulfomicrobiaceae) were identified having significant influences on Cd speciation transformation and they could stabilize Cd into sulfide precipitation through dissimilatory sulfate reduction (DSR). The ANOVA results of mobilization index and Cd concentration in overlying water both demonstrated that integrated-remediation systems with 5 mg/g and 10 mg/g of nZVI (Fe5 and Fe10 systems, respectively) presented better immobilization performance than conventional SRB only system (P < 0.05). It is confirmed that nZVI could stimulate the SRB bio-immobilization possibily through providing electrons and enhancing enzyme activities during DSR. The XPS analyses and Pourbaix diagrams revealed that mackinawite may be produced in the Fe10, resulting in the possible formation of Cd-S-Fe. This study indicates that integrated-remediation of SRB and nZVI have great potential in Cd immobilization of sediments, especially with higher addition of nZVI.

13.
Environ Pollut ; 270: 115721, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33321439

RESUMO

Increasing concerns over bisphenol A (BPA) as an endocrine disrupting chemical (EDC) and its adverse effects on both humans and animals have led to the substitution by structural analogs, such as bisphenol F (BPF), in many application areas. Information regarding to the carry-over of this emerging chemical in farm animals is essential for legislation and risk assessment purposes. In this study, a large-scale number of animal experiments were designed to investigate the transfer of BPF from feed to eggs. One control and three experimental groups of laying hens (72 hens per group) were fed with basal diets and BPF-contaminated feed at concentration levels of 0.1, 0.5 and 2.5 mg kg-1, respectively, for two weeks. The hens were then fed with BPF-free diets for a further four weeks. Eggs were collected daily, and separated into egg yolk and white for BPF analysis. The effects of different levels of BPF exposure on laying performance followed a non-monotonic dose-response curve, since low level BPF (0.1 mg kg-1) exposure did increase the laying rate, mean egg weight and daily feed intake, while high level BPF (2.5 mg kg-1) exposure showed a decreasing trend. BPF residues were detected in both egg yolks and whole eggs after two days of administration, and plateau phase was achieved within 9-18 days. There are clear linear dose-response relationships between the plateau BPF concentrations in feed and eggs. The residue of BPF was found mainly in egg yolks with conjugated form and depleted slowly (still detected 21 days after feeding the BPF-free diet of the high level group). Mean carry-over rate of 0.59% BPF from feed to eggs was obtained. Compared with the carry-over rates of PCBs and dioxins, BPF showed a relatively minor trend of bioaccumulation in eggs. To the best of our knowledge, this is the first report on the deposition, depletion, and bioaccumulation study of bisphenols in farm animals. The quantity of data can therefore be helpful in the frame of risk assessment, especially for a comprehensive estimation of consumer exposure to the residues of bisphenols.


Assuntos
Ração Animal , Galinhas , Ração Animal/análise , Animais , Compostos Benzidrílicos , Bioacumulação , Dieta , Ovos , Feminino , Humanos , Fenóis
14.
J Environ Sci (China) ; 100: 90-98, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33279057

RESUMO

Nitrate (NO3-) is known to be actively involved in the processes of mineralization and heavy metal transformation; however, it is unclear whether and how it affects the bioavailability of antimony (Sb) in paddy soils and subsequent Sb accumulation in rice. Here, the effects of NO3- on Sb transformation in soil-rice system were investigated with pot experiments over the entire growth period. Results demonstrated that NO3- reduced Sb accumulation in brown rice by 15.6% compared to that in the control. After amendment with NO3-, the Sb content in rice plants increased initially and then gradually decreased (in roots by 46.1%). During the first 15 days, the soil pH increased, the oxidation of Sb(III) and sulfides was promoted, but the reduction of iron oxide minerals was inhibited, resulting in the release of adsorbed and organic-bound Sb from soil. The microbial arsenite-oxidizing marker gene aoxB played an important role in Sb(III) oxidation. From days 15 to 45, after NO3- was partially consumed, the soil pH decreased, and the reductive dissolution of Fe(III)-bearing minerals was enhanced; consequently, iron oxide-bound Sb was transformed into adsorbed and dissolved Sb species. After day 45, NO3- was completely reduced, Sb(V) was evidently reduced to Sb(III), and green rust was generated gradually. Thus, the available Sb decreased due to its enhanced affinity for iron oxides. Moreover, NO3- inhibited the reductive dissolution of iron minerals, which ultimately caused low Sb availability. Therefore, NO3- can chemically and biologically reduce the Sb availability in paddy soils and alleviate Sb accumulation in rice. This study provides a potential strategy for decreasing Sb accumulation in rice in the Sb-contaminated sites.


Assuntos
Oryza , Poluentes do Solo , Antimônio/análise , Disponibilidade Biológica , Compostos Férricos , Nitratos , Raízes de Plantas/química , Solo , Poluentes do Solo/análise
15.
Chemosphere ; : 129055, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33272672

RESUMO

Polychlorinated biphenyls (PCBs) and dibenzo-p-dioxins and furans (PCDD/Fs) are notorious persistent organic pollutants (POPs), which may bioaccumulate through food chain and play detrimental effects to organisms even at trace levels. Quantification of PCBs and PCDD/Fs in biotic samples is a great challenge. In the present study, gas chromatography coupled to triple quadrupole mass spectrometry with an atmospheric pressure chemical ionization source (GC-(APCI)MS/MS) was studied for the isotope-dilution analysis of PCBs (mono-to deca-) and PCDD/Fs in Chinese mitten crab food webs. High-resolution mass spectrometry (HRMS) was applied for comparison. Light PCBs are compared between the two instruments for the first time. After optimization of instrument parameters, the RSDs of relative response factors of calibration curves were between 3.4% and 15.5% for PCBs and 1.7%-7.9% for PCDD/Fs. The limits of detection were between 0.021 and 0.150 pg/mL for PCBs and 0.051-0.237 pg/mL for PCDD/Fs. PCB concentrations in crab food web samples detected by GC-(APCI)MS/MS were well correlated with those detected by HRGC/HRMS. A DiCB, 3,3'-dichlorobiphenyl (PCB11), was the dominant PCB congener in aquatic food webs. Other MoCB and DiCB congeners were also widely identified; hence, low-weight PCB congeners should arouse more concern in the future. GC-(APCI)MS/MS may become an alternative instrument satisfying the PCB and PCDD/F detection.

16.
Trauma Violence Abuse ; : 1524838020975895, 2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33267741

RESUMO

During the past decade, research on the link between childhood emotional maltreatment and adulthood romantic relationship well-being has been accumulating, but there still lacks a systematic, quantitative evaluation of existing research. This three-level, meta-analysis aimed to fill this gap. Reports were included if they examined the link between early emotional maltreatment and adulthood romantic relationship well-being, presented statistics needed to calculate at least one bivariate effect size, written in English, and published/written before January 1, 2020. We retrieved 201 effect sizes from 23 reports. Early emotional maltreatment (aggregated across forms) was negatively (yet modestly) associated with later romantic relationship well-being (aggregated across dimensions; r = -.143, 95% confidence interval [-.173, -.114], p < .001). This association did not vary as a function of maltreatment form but differed across relationship well-being dimensions, such that the effect was stronger for the negative than for the positive relationship outcomes. We also found that (a) the actor effect was larger than the partner effect, (b) the effect was stronger in studies using Childhood Trauma Questionnaire (CTQ) than in studies not using CTQ, (c) whether using established measures of relationship well-being did not alter the effect, (d) the absolute magnitude of effect was negatively associated with methodological rigor of effect, and (e) the effect did not vary as functions of publication type, whether the sample was a college student sample, or union status, and was not related to the mean of union duration. Last, the limitations of existing research, avenues for future inquiries, and implications for practice were noted.

17.
Diagn Pathol ; 15(1): 141, 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33308276

RESUMO

BACKGROUND: The involvement of circular RNAs (circRNAs) in chemoresistance of tumors has been identified. Herein, this study aims to investigate the role and the underlying mechanism of circ_0003998 in doxorubicin (DOX) resistance in hepatocellular carcinoma (HCC). METHODS: The expression of circ_0003998 and microRNA (miR)-218-5p and eukaryotic translation initiation factor 5A-2 (EIF5A2) mRNA was detected using quantitative real-time polymerase chain reaction. Cell viability, migration and invasion were analyzed using cell counting kit-8, colony formation and transwell assay, respectively. The levels of matrix metallopeptidase 9 (MMP-9), E-cadherin, Vimentin, N-cadherin and EIF5A2 protein were detected using western blot. The interaction between miR-218-5p and circ_0003998 or EIF5A2 was confirmed by dual-luciferase reporter assay. In vivo experiments were performed using murine xenograft models. RESULTS: Circ_0003998 was elevated in HCC tissues, DOX-resistant tissues and cells, and circ_0003998 knockdown promoted DOX-sensitivity in HCC by inhibiting resistant cell viability, migration, invasion and EMT in vitro and enhanced DOX cytotoxicity in vivo. Bioinformatics analysis revealed circ_0003998 inhibited miR-218-5p expression, which was clarified to be a target of circ_0003998, and circ_0003998 knockdown sensitized HCC cell to DOX by sponging miR-218-5p. EIF5A2 was a target of miR-218-5p, and miR-218-5p mitigated DOX resistance in HCC cells through modulating EIF5A2 expression. Additionally, circ_0003998 served as a competing endogenous RNA for miR-218-5p to regulate EIF5A2 expression. CONCLUSION: Circ_0003998 knockdown sensitized HCC cell to DOX by regulating miR-218-5p/EIF5A2 axis, indicating new markers of poor response to DOX and potential therapeutic strategies for the chemotherapy of HCC.

18.
J Am Chem Soc ; 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33141579

RESUMO

Bending and folding are important stereoscopic geometry parameters of one-dimensional (1D) nanomaterials, yet the precise control of them has remained a great challenge. Herein, a surface-confined winding assembly strategy is demonstrated to regulate the stereoscopic architecture of uniform 1D mesoporous SiO2 (mSiO2) nanorods. Based on this brand-new strategy, the 1D mSiO2 nanorods can wind on the surface of 3D premade nanoparticles (sphere, cube, hexagon disk, spindle, rod, etc.) and inherit their surface topological structures. Therefore, the mSiO2 nanorods with a diameter of ∼50 nm and a variable length can be bent into arc shapes with variable radii and radians, as well as folded into 60, 90, 120, and 180° angular convex corners with controllable folding times. Additionally, in contrast to conventional core@shell structures, this winding structure induces partial exposure and accessibility of the premade nanoparticles. The functional nanoparticles can exhibit large accessible surface and efficient energy exchanges with the surroundings. As a proof of concept, winding-structured CuS&mSiO2 nanocomposites are fabricated, which are made up of a 100 nm CuS nanosphere and the 1D mSiO2 nanorods with a diameter of ∼50 nm winding the nanosphere in the perimeter. The winding structured nanocomposites are demonstrated to have fourfold photoacoustic imaging intensity compared with the conventional core@shell nanostructure with an inaccessible core because of the greatly enhanced photothermal conversion efficiency (increased by ∼30%). Overall, our work paves the way to the design and synthesis of 1D nanomaterials with controllable bending and folding, as well as the formation of high-performance complex nanocomposites.

19.
Food Chem ; : 128331, 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33097326

RESUMO

Artificial sweeteners have been widely used as replacements for sugars in foods. Rapid determination of artificial sweeteners contained in various foods are highly desirable for the routine analysis. Here, we report a robust approach based on direct analysis in real time coupled with QTRAP mass spectrometry to screen and quantitate simultaneously seven artificial sweeteners, including aspartame, saccharin, acesulfame-K, neotame, sucralose, cyclamate and alitame in alcoholic drinks. The detection method merely involved a simple sample pretreatment process, with a good linearity, low limit of quantification, satisfied recovery and relative standard deviation for each target compound. More importantly, the approach is highly sensitive and accurate in monitoring the seven artificial sweeteners in whisky, Chinese liquors, beer and wines obtained from the supermarket. The results demonstrated that the approach described here could be suitable for large-scale application in routine quality control analysis of artificial sweeteners.

20.
Fam Process ; 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33073858

RESUMO

An emerging (yet still scant) body of research has linked interparental hostility to youth compromised social competence over time among adolescents. Moreover, little is known about the conditions under which and the processes through which this association might occur. Using prospective data from 878 youth (50.23% females) and their parents and teachers, this study examined how interparental hostility and cooperative conflict might work in conjunction with each other to predict youth social competence over time via parent-child relationship quality. Results demonstrated that interparental cooperative conflict at grade 5 buffered the negative association between interparental hostility at grade 5 and mother-child but not father-child relationship quality at grade 6. Mother-child relationship quality, in turn, was associated positively with youth social competence at age 15. As such, interparental hostility at grade 5 was negatively related to youth social competence at age 15 via mother-child relationship quality at grade 6 only when interparental cooperative conflict at grade 5 was low. This study represents a more nuanced and specific examination of the implications of interparental hostility for child later social development by highlighting underlying moderating and mediating mechanisms. Relevant implications for the development of more targeted and effective interventions are also discussed.

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