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1.
Ecotoxicology ; 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33433766

RESUMO

Anammox bacteria have been detected in various ecosystems, but their occurrence and community composition along the shipping channels have not been reported. In this study, anammox bacteria were recovered by PCR-amplified biomarker hzsB gene from the genomic DNA of the sediment samples. Phylogenetic tree revealed that Candidatus Scalindua and Ca. Brocadia dominated the anammox community of the Hong Kong channels; Ca. Scalindua spp. was present abundantly at the sites farther from the shore, whereas Ca. Jettenia and Ca. Kuenenia were detected as the minor members in the estuarine sediments near the shipping terminals. The highest values of Shannon-Wiener index and Chao1 were identified in the sediments along the Urmston road (UR), suggesting the highest α-diversity and species richness of anammox bacteria. PCoA analysis indicated that anammox bacterial communities along UR and Tai Hong (TH) channel were site-specific because these samples were grouped and clearly separated from the other samples. The maximum diversity of anammox bacteria was detected in UR samples, ranging from 6.28 × 105 to 1.28 × 106 gene copies per gram of dry sediment. A similar pattern of their transcriptional activities was also observed among these channels. Pearson's moment correlation and redundancy analysis indicated that NH4+-N was a strong factor shaping the community structure, which showed significant positive correlation with the anammox bacterial abundance and anammox transcriptional activities (p < 0.01, r > 0.8). Also, NH4+-N, (NO3- + NO2-)-N, and NH4+/NOX were additional key environmental factors that influenced the anammox community diversity and distribution. This study yields a better understanding of the ecological distribution of anammox bacteria and the dominant genera in selective niche.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33400127

RESUMO

Nitrate (NO3-) and nitrous oxide (N2O) accumulate in groundwater in relation to human activities and pose multiple threats to the global environment (harming human health and atmospheric damage). This study focused on the evaluation of groundwater NO3-, N2O, and its indirect emission factor under different land use types (agricultural land, urban land, and forest) and response mechanism of major anions to dissolved N2O within groundwater in Dexing which has the largest copper mine in Asia. Specifically, this work used self-organizing maps (SOMs) to identify which anion conditions (NO3-, SO42-, F-, Cl-) and water quality parameters were suitable for the accumulation of groundwater N2O. Finally, we found that the shallow groundwater of agricultural land has a high concentration of NO3- and N2O and the agricultural activity has a significant effect on the temporal and spatial variation of N2O in groundwater. The result of SOMs combined with the positive correlation between N2O and NO3-/SO42- suggested that the electron donor required for denitrification has a significant effect on N2O accumulation. In this respect, when an increased proportion of reduced sulfur is available as an electron donor for autotrophic denitrification, this results in lower concentrations of N2O in groundwater. Through the comprehensive evaluation of the anion conditions and N2O in groundwater under different land use types, this study case can help to estimate the N2O indirect emission from groundwater, so as to constrain the global nitrogen budget.

3.
Ann Epidemiol ; 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33422600

RESUMO

BACKGROUND AND OBJECTIVE: Young men who have sex with men (YMSM) are likely to experience depression due to enacted and internalized stigma. Whether enacted stigma impacts depression through internalized stigma and whether resilience moderate the negative effect are unclear. This study aims to examine a moderated mediation model to explore the role of internalized stigma in mediating the relationship between enacted stigma and depressive symptoms, and whether this relationship is modified by resilience among Chinese YMSM. METHODS: Between September 2017 and January 2018, we conducted a baseline survey of a four-year cohort study among MSM in China. An anonymous computer-assisted ("Questionnaire Star") self-interview questionnaire survey was conducted and 346 YMSM were included for analysis. Sociodemographic characteristics, enacted stigma, internalized stigma, resilience, and depressive symptoms were measured. Mediation and moderated mediation model analysis were used to examine the relationships among these variables. RESULTS: Overall, 38.7% (137) participants reported depressive symptoms. Internalized stigma partly mediated the relationship between enacted stigma and depressive symptoms (ß=0.091, P<0.01). Moderation mediation analysis found positive moderating role of resilience in the relationship between enacted stigma and internalized stigma (ß=-0.026, P=0.019), as well as between enacted stigma and depressive symptoms (ß=-0.053, P=0.008). The indirect effects of enacted stigma on depressive symptom through internalized stigma were significant only when resilience was at low (ß= 0.191, 95% CI=0.072,0.366) and moderate (ß=0.104, 95% CI=0.035,0.220) levels, but not significant when resilience was high (ß=0.017, 95% CI=-0.083, 0.140). CONCLUSIONS: Enacted stigma could directly impact depressive symptoms, and internalized stigma partly mediated the relationship between enacted stigma and depressive symptoms. Resilience significantly moderated the association between enacted stigma and depressive symptoms as well as the association between enacted stigma and internalized stigma. Promoting resilience while reducing enacted stigma and internalized stigma may be useful in relieving depressive symptoms among Chinese YMSM.

4.
J Cell Mol Med ; 25(1): 27-36, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33128338

RESUMO

The effects of long-term nitrate therapy are compromised due to protein S-Nitrosylation, which is mediated by nitric oxide (NO). This study is to determine the role of Akt S-Nitrosylation in the recovery of heart functions after ischaemia. In recombinant Akt protein and in HEK293 cells, NO donor decreased Akt activity and induced Akt S-Nitrosylation, but was abolished if Akt protein was mutated by replacing cysteine 296/344 with alanine (Akt-C296/344A). In endothelial cells, NO induced Akt S-Nitrosylation, reduced Akt activity and damaged multiple cellular functions including proliferation, migration and tube formation. These alterations were ablated if cells expressed Akt-C296/344A mutant. In Apoe-/- mice, nitroglycerine infusion increased both Akt S-Nitrosylation and infarct size, reduced Akt activity and capillary density, and delayed the recovery of cardiac function in ischaemic hearts, compared with mice infused with vehicle. Importantly, these in vivo effects of nitroglycerine in Apoe-/- mice were remarkably prevented by adenovirus-mediated enforced expression of Akt-C296/344A mutant. In conclusion, long-term usage of organic nitrate may inactivate Akt to delay ischaemia-induced revascularization and the recovery of cardiac function through NO-mediated S-Nitrosylation.

5.
Talanta ; 221: 121459, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076081

RESUMO

Herein, a novel sandwich-type electrochemical immunosensor was fabricated based on Fe3O4 nanoflowers (Fe3O4 NFs) and heterogeneous chain reaction (HCR) signal amplification strategy for the sensitive detection of hepatitis B surface antigen (HBsAg). The aldehyde-functionalized Fe3O4 NFs are used as a supporting matrix to immobilize the hepatitis B surface antibody 1 (HBsAb1). The biotin-modified single-strand DNA (biotin-S0) was connected onto the biotin-HBsAb2 via linkage of streptavidin (SA), followed by addition of methylene blue (MB) modified single strand DNA1 (MB-S1) and DNA2 (MB-S2) for HCR signal amplification. The designed immunosensor exhibited a detection linear range of 0.5 pg mL-1-0.25 ng mL-1 and a low detection limit of 0.16 pg mL-1, with excellent stability, selectivity and reproducibility. Furthermore, HBsAg is detected in the serum samples with a stable and fast response, indicating that the proposed immunosensor has a promising potential application in clinical analysis.

6.
Sci Total Environ ; 755(Pt 2): 142632, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33045611

RESUMO

High concentrations of antibiotics can exert strong selection pressures on the microbial community and promote the emergence and dissemination of antibiotic resistance genes (ARGs). The activated sludge reactors treating ampicillin, cephalexin and chloramphenicol production wastewater were established to investigate the responses of microbial community, ARGs and mobile genetic elements (MGEs) to antibiotics. Antibiotic selection pressures significantly declined the microbial diversity and changed microbial community structures. Based on metagenomic analysis, a total of 500 ARG subtypes affiliated with 18 ARG types were identified and 63 ARGs were shared by all samples. The substantial increase of ARG abundance and the shifts of ARG profiles were significantly correlated with antibiotic types and concentrations. The evident enrichment of non-corresponding ARG types suggested the strong co-selection effects of the target antibiotics. Additionally, metagenomic analysis revealed the occurrence of 104 MGEs belonging to various types and the five dominant MGEs were tnpA, intI1, tniA, tniB and IS91. The ARG-MGE co-occurrence associations implied the potential mobility of ARGs. Network analysis also demonstrated that five ARG types (aminoglycoside, beta-lactam, chloramphenicol, multidrug and tetracycline resistance genes) tended to co-occur internally and the obvious co-occurrence patterns among different ARG types indicated the potential for resistance co-selection. Moreover, 15 bacterial genera were speculated as the hosts of diverse ARGs. This study provides a comprehensive overview of the occurrence of ARGs and MGEs and is valuable for the risk assessment and management of antibiotic resistance.


Assuntos
Microbiota , Esgotos , Ampicilina , Antibacterianos/farmacologia , Cefalexina , Cloranfenicol , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos
7.
Ecotoxicol Environ Saf ; 207: 111521, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33254396

RESUMO

Phthalates (PAEs), bisphenol A (BPA), and oestrogenic compounds have become major concerns due to their endocrine-disrupting effect. However, few studies related to the occurrence of PAEs, BPA, and oestrogen in food and compost from different growth age livestock have been conducted. In this study, faeces, urine and food samples were collected from a typical livestock (cow) and a special livestock (pigeon) from concentrated animal feeding operations (CAFOs). The daily total oestrogen excretion of a single cow ranged from 192 µg/day to 671 µg/day, which was significantly higher than that of a single pigeon (0-0.01 µg/day). Conjugated oestrogens represented 22.0-46.0% of the total oestrogens excreted from cow faeces and 80.7-91.8% of those from cow urine, indicating that the form of the excreted oestrogens depends on the livestock species and type of excrement. BPA was all detected in all livestock manure and food, and the concentration in pigeon was 9.2-40.2 ng/g and 23.1 ng/g respectively, while that in cattle was 50.5-72.0 ng/g and 41.1 ng/g respectively. The results indicated that the food is significant sources of BPA entering the process of cow and pigeon breeding. Diethyl phthalate (DEP) was detected at high frequency in pigeon faeces samples, suggesting that pigeons were highly exposed to these plasticisers. The total oestradiol equivalent quantity (EEQt) of livestock origin in aquatic environments was estimated to be 2.99 ng/L, which was higher than the baseline hazard value (1 ng/L) (Xu et al., 2018). The study provides data on the emissions and sources of PAEs, BPA, and oestrogenic compounds from different livestock in CAFOs and demonstrates that food is a significant source of BPA entering livestock.


Assuntos
Ração Animal/análise , Compostos Benzidrílicos/análise , Poluentes Ambientais/análise , Estrogênios/análise , Contaminação de Alimentos/análise , Fenóis/análise , Ácidos Ftálicos/análise , Animais , Bovinos , China , Fezes/química , Feminino , Contaminação de Alimentos/estatística & dados numéricos , Humanos , Gado , Esterco/análise , Plastificantes
8.
Polymers (Basel) ; 12(12)2020 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-33260397

RESUMO

In this paper, CNTs@PANi nanocomposites were prepared by in-situ oxidation polymerization of aniline, and their structure, morphology and conductivity were characterized. A mixed solvent of toluene and tetrahydrofuran was used to prepare dispersions of CNTs@PANi and poly(styrene-ethylene-butylene-styrene) (SEBS) triblock copolymer, and bilayer composite film was prepared. According to the solvent phase separation and uneven evaporation flux, CNTs@PANi self-assembled into the interconnected coffee ring structure on the SEBS matrix. The prepared bilayer composite film had excellent stretchability, and the conductivity of the functional layer was close to that of CNTs@PANi, which could light up an LED lamp under 100% strain and restore the topological structure. Electrochemical tests showed that the bilayer film had obvious heterogeneity. The impedance characteristics of the CNTs@PANi functional layer and the SEBS matrix were analyzed, and its heterogeneous corrosion resistance mechanism further discussed.

9.
Front Neurol ; 11: 591605, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33281732

RESUMO

Objective: Initial randomized controlled trials (RCTs) and recently released systematic reviews have identified resistance training (RT) as a modality to manage motor symptoms and improve physical functioning in individuals with Parkinson's disease (PD), although the effects are inconsistent. Therefore, we conducted an updated meta-analysis to reassess the evidence of the relationship. Methods: We performed a systematic search of studies reporting the effects of RT in PD available through major electronic databases (PubMed, Medline, Embase, Ovid, Cochrane Library, CNKI, Wanfang) through 20 July 2020. Eligible RCTs were screened based on established inclusion criteria. We extracted data on the indicators of leg strength, balance, gait capacity, and quality of life (QoL) of lower limbs. Random and fixed effects models were used for the analysis of standard mean differences (SMD) or mean differences (MD) with their 95% confidence intervals (CI). Results: Thirty-one papers from 25 independent trials compromising 1,239 subjects were selected for eligibility in this systematic review and meta-analysis. Summarized data indicated that the leg strength increased statistically significant in PD patients (SMD = 0.79, 95% CI 0.3, 1.27, P = 0.001), the balance capability was improved statistically significant in PD patients (SMD = 0.34, 95% CI 0.01, 0.66, P = 0.04), and QoL statistically significantly improved (MD = -7.22, 95% CI -12.05, -2.39, P = 0.003). For gait performance, four indicators were measured, the results as follows: fast gait velocity (MD = 0.14, 95% CI 0.06, 0.23, P = 0.001), Timed-up-and-go-test (TUG, MD = -1.17, 95% CI -2.27, -0.08, P = 0.04) and Freezing of Gait Questionnaire (FOG-Q, MD = -1.74, 95% CI -3.18, -0.3, P = 0.02) were improved statistically significant across trials, while there were no statistically significant improvement in stride length (MD = -0.05, 95% CI -0.12, 0.02, P = 0.15) in PD patients. Conclusions: Lower limb RT has positive effects during rehabilitation in individuals with PD in leg strength, QoL, and improve gait performance to a certain extent. RT also could improve balance capacity of patients, although a wide variety of tools were used, and further study is needed to confirm these findings.

10.
Sensors (Basel) ; 20(24)2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33302357

RESUMO

Accurate prediction of wetland soil organic carbon concentration and an understanding of its controlling factors are important for studying regional climate change and wetland carbon cycles; with that knowledge mechanisms can be put in place that are conducive to sustainable ecosystem management for environmental health. In this study, a hybrid approach combining an artificial neural network and ordinary kriging and 103 soil samples at three soil depth ranges (0-30, 30-60, and 60-100 cm) were used to predict wetland soil organic carbon concentration in China's Liao River Basin. The model evaluation indicated that a combination of artificial neural network and ordinary kriging and limited soil samples achieved good performance in predicting wetland soil organic carbon concentration. Wetland soil organic carbon concentration in the Liao River Basin has apparent spatial and vertical heterogeneities with values decreasing from southeast to northwest and concentrates present mainly in the topsoil (0-30 cm). Mean wetland soil organic carbon concentration values at the three soil depths were 10.43 ± 0.38, 7.93 ± 0.25, and 7.61 ± 0.22 g/kg, respectively, which are smaller than those over other wetland regions in Northeast China. Terrain aspect contributed the most in predicting wetland soil organic carbon concentration at each of the three soil depths, followed by normalized difference vegetation index at 0-30 cm and mean annual precipitation at 30-60 and 60-100 cm. This study provides a framework method and baseline to quantify the soil organic carbon concentration dynamics in response to climatic and anthropogenic drivers.

11.
Immun Inflamm Dis ; 2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33270996

RESUMO

INTRODUCTION: Transcriptional regulation of autophagy depends on the transcription factors coordinated inflammatory feedback mechanism. Here, we provide a comprehensive functional characterization of periodontal ligament fibroblasts (PDLFs) treated with Porphyromonas gingivalis lipopolysaccharide (LPS), aiming to reveal previously unappreciated biological changes and to investigate how a transcription factor differentiated embryonic chondrocytes 2 (Dec2)-deficient environment influences the function of autophagy in nflamed human PDLFs. METHODS: A Dec2-deficient (Dec2KO) experimental periodontal inflammation mouse model and treatment with P. gingivalis LPS were employed to examine the role of autophagy in PDLFs using hematoxylin and eosin staining and immunohistochemistry in vivo. A Dec2 small interfering RNA (siRNA) was used to modulate autophagy, and the effect of autophagy on the Dec2 pathway was explored using real-time polymerase chain reaction and western blot analysis in vitro. RESULTS: LPS-treated human PDLFs (HPDLFs) induced autophagy, as demonstrated by the enhanced levels of microtubule-associated protein 1 light chain 3-II (LC3-II) and the induction of ATG5, Beclin1, and Dec2. Compared with a scrambled siRNA, a Dec2 siRNA triggered the detrimental influences of LPS and markedly enhanced autophagy expression in inflamed HPDLFs. The expression of phosphorylated ERK was increased and levels of phosphorylated mammalian target of rapamycin (mTOR) were decreased after exposure to LPS in Dec2 siRNA transfected HPDLFs. The Dec2KO model exhibited that P. gingivalis in Dec2 deficient conditions increases the inflammation of PDLFs by regulating autophagy. CONCLUSIONS: These results demonstrate that a Dec2 deficiency can alleviate LPS-induced inflammation via the ERK/mTOR signaling pathway by regulating autophagy, conceivably delivering a novel approach for the detection of periodontal treatments.

12.
JAMA Psychiatry ; 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33263727

RESUMO

Importance: The genetic basis of bipolar disorder (BD) in Han Chinese individuals is not fully understood. Objective: To explore the genetic basis of BD in the Han Chinese population. Design, Setting, and Participants: A genome-wide association study (GWAS), followed by independent replication, was conducted to identify BD risk loci in Han Chinese individuals. Individuals with BD were diagnosed based on DSM-IV criteria and had no history of schizophrenia, mental retardation, or substance dependence; individuals without any personal or family history of mental illnesses, including BD, were included as control participants. In total, discovery samples from 1822 patients and 4650 control participants passed quality control for the GWAS analysis. Replication analyses of samples from 958 patients and 2050 control participants were conducted. Summary statistics from the European Psychiatric Genomics Consortium 2 (PGC2) BD GWAS (20 352 cases and 31 358 controls) were used for the trans-ancestry genetic correlation analysis, polygenetic risk score analysis, and meta-analysis to compare BD genetic risk between Han Chinese and European individuals. The study was performed in February 2020. Main Outcomes and Measures: Single-nucleotide variations with P < 5.00 × 10-8 were considered to show genome-wide significance of statistical association. Results: The Han Chinese discovery GWAS sample included 1822 cases (mean [SD] age, 35.43 [14.12] years; 838 [46%] male) and 4650 controls (mean [SD] age, 27.48 [5.97] years; 2465 [53%] male), and the replication sample included 958 cases (mean [SD] age, 37.82 [15.54] years; 412 [43%] male) and 2050 controls (mean [SD] age, 27.50 [6.00] years; 1189 [58%] male). A novel BD risk locus in Han Chinese individuals was found near the gene encoding transmembrane protein 108 (TMEM108, rs9863544; P = 2.49 × 10-8; odds ratio [OR], 0.650; 95% CI, 0.559-0.756), which is required for dendritic spine development and glutamatergic transmission in the dentate gyrus. Trans-ancestry genetic correlation estimation (ρge = 0.652, SE = 0.106; P = 7.30 × 10-10) and polygenetic risk score analyses (maximum liability-scaled Nagelkerke pseudo R2 = 1.27%; P = 1.30 × 10-19) showed evidence of shared BD genetic risk between Han Chinese and European populations, and meta-analysis identified 2 new GWAS risk loci near VRK2 (rs41335055; P = 4.98 × 10-9; OR, 0.849; 95% CI, 0.804-0.897) and RHEBL1 (rs7969091; P = 3.12 × 10-8; OR, 0.932; 95% CI, 0.909-0.956). Conclusions and Relevance: This GWAS study identified several loci and genes involved in the heritable risk of BD, providing insights into its genetic architecture and biological basis.

13.
Ann Pharmacother ; : 1060028020979773, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33345552

RESUMO

OBJECTIVE: To review medical management of inoperable malignant bowel obstruction. DATA SOURCES: A literature review using PubMed and MEDLINE databases searching malignant bowel obstruction, etiology, types, pathophysiology, medical, antisecretory, anti-inflammatory, antiemetic drugs, analgesics, promotion of emptying, prevention of infection, anticholinergics, somatostatin analogs, gastric antisecretory drugs, prokinetic agents, glucocorticoid, opioid analgesics, antibiotics, enema, and adverse effects. STUDY SELECTION AND DATA EXTRACTION: Randomized or observational studies, cohorts, case reports, or reviews written in English between 1983 and November 2020 were evaluated. DATA SYNTHESIS: Malignant bowel obstruction (MBO) commonly occurs in patients with advanced or recurrent malignancies and severely affects the quality of life and survival of patients. Its management remains complex and variable. Medical management is the cornerstone of MBO treatment, with the goal of reducing distressing symptoms and optimizing quality of life. Until now, there has been neither a standard clinical approach nor registered medications to treat patients with inoperable MBO. RELEVANCE TO PATIENT CARE AND CLINICAL PRACTICE: This review provides information on the etiology, type and pathophysiology, and medical treatment of MBO and related adverse reactions of the drugs commonly used, which can greatly assist clinicians in making clinical decisions when treating MBO. CONCLUSIONS: Published research shows that medical management of MBO mainly consists of antisecretory, anti-inflammatory strategies, controlling vomiting and pain, promoting emptying, preventing infection, and combination therapy. Being knowledgeable about the most current treatment options, the related adverse effects, and the evidence supporting different practices is critical for clinicians to provide individualized medical therapy for MBO patients.

15.
BMC Public Health ; 20(1): 1867, 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33287780

RESUMO

BACKGROUND: Individuals' stress in responding to the current COVID-19 pandemic may be exacerbated by information uncertainty driven by inconsistent, unverified, and conflicting news from various sources. The current study aims to test if information uncertainty during the COVID-19 outbreak was related to acute stress disorder (ASD) over and above other psychosocial stressors. METHODS: An anonymous online survey was conducted with 7800 college students throughout China from January 31 through February 11, 2020. Existing scales were modified to measure ASD and six potential stressors including information uncertainty during the COVID-19 outbreak. Hierarchical regression analysis was conducted to assess the unique association of information uncertainty with ASD. To minimize the effect of large sample size and also to get a sense of whether the effects of information uncertainty were similar to people at the center of the epidemic, we repeated the hierarchical regression among 10% of the students who were randomly selected from the entire sample ("10% random sample"; n = 780) and 226 students from Hubei Province where the outbreak started. RESULTS: Information uncertainty was highly prevalent among the respondents (64%). It was significantly associated with ASD beyond other key variables and potential stressors across three samples. In the hierarchical regression among the entire sample, demographic variables accounted for 9.4% of the variance in ASD. The other five stressors added 5.1% of the variance. The information uncertainty (ß = .159; p < .001) explained an additional 2.1% of the variance. Likewise, the information uncertainty explained an additional 2.1 and 3.4% of the variance in ASD beyond all other variables among the 10% random sample (ß = .165; p < .001) and the Hubei sample (ß = .196; p < .01), respectively. CONCLUSION: Information uncertainty is a unique correlate of psychological stress during the COVID-19 outbreak. Reducing information uncertainty is essential not only for halting virus transmission but also for mitigating negative impacts of the pandemic on people's psychosocial wellbeing. Transparent, timely, and accurate communication can reduce public confusion, fear, and stress. Capacity building in governments, communities, and media outlets to prevent, reduce and manage information uncertainty should be a critical part of the response to an emerging global health crisis such as the COVID-19 pandemic.


Assuntos
/psicologia , Informação de Saúde ao Consumidor/normas , Surtos de Doenças , Transtornos de Estresse Traumático Agudo/epidemiologia , Incerteza , Adolescente , China/epidemiologia , Feminino , Humanos , Masculino , Fatores de Risco , Estresse Psicológico/epidemiologia , Estudantes/psicologia , Estudantes/estatística & dados numéricos , Inquéritos e Questionários , Adulto Jovem
16.
Zootaxa ; 4868(3): zootaxa.4868.3.7, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33311394

RESUMO

Two new species of the genus Scaphidium Olivier from Xizang (Tibet Autonomous Region), China are described: 1) S. rhopaliparamerosum sp. nov. [], distinguished by four dark red big fasciae on apical 1/3 and basal 1/3 parts of elytra; and 2) S. russipenne sp. nov. [], distinguished by the unified brownish red colour on elytra. For both new species, we provide descriptions and colour plates of normal light photos for the diagnostic characters: habitus in both dorsal and ventral views, an antenna, a prefemur, and the detailed structures of the aedeagus (if male available). Additionally, a new spot pattern of S. formosanum Pic is reported from Yunnan.


Assuntos
Besouros , Distribuição Animal , Estruturas Animais , Animais , China , Masculino
17.
J Comput Chem ; 2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33326120

RESUMO

A detailed reaction mechanism of acetylene cyclotrimerization catalyzed by V(i PrNPMe2 )3 Fe-PMe3 (denote as CAT), a heterobimetallic complex featuring V-Fe triple bond, was computationally investigated using density functional theory. The calculated results show that the first acetylene firstly attaches to the V atom of CAT to get a four-membered ring structure through [2 + 2] cycloaddition reaction. For the second acetylene addition, there are two cyclotrimerization mechanisms, outer sphere mechanism and inner mechanism. The inner sphere reaction pathway is the main reaction pathway. By replacing the V with Nb and Ta, Fe with Ru and Os, a series of new catalysts are screened computationally. The calculated results show that, all of the nine heterobimetallic complexes show high activity at mild condition. The energy barrier of the rate determining step is related to the natural population analysis (NPA) charge of M' and the Wiberg bond index (WBI) of M-M' bond. The more negative NPA charge of M' and the smaller WBI of M-M' bond, the lower energy barrier is.

18.
Ther Adv Med Oncol ; 12: 1758835920970049, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33224276

RESUMO

Background: Targeting immune checkpoints represents an immense breakthrough in cancer therapeutics. The prognostic value of hemoglobin (Hb) has been investigated in many malignancies including non-small cell lung cancer (NSCLC). However, the prognostic impact of pretreatment Hb count for immune checkpoint inhibitors (ICIs) in advanced NSCLC patients remains unclear. Methods: A total of 310 late-stage NSCLC patients who received ICI therapies between January 2015 and March 2019 were prospectively enrolled. We used a propensity score-matched cohort analysis for this study. Patients' clinicopathological characteristics and pretreatment Hb concentration were assessed against the progression-free survival (PFS) and overall survival (OS) using the Kaplan-Meier method and Cox proportional hazards regression. Results: A propensity score (PS)-matched cohort analysis was applied to adjust for potential bias and to create two comparable groups according to patients' clinicopathological characteristics. The patients with normal baseline Hb levels (⩾110 g/L) had significantly longer PFS [median: 10.0 versus 4.0 months, hazard ratio (HR): 0.63, 95% confidence interval (CI): 0.46-0.86; p = 0.001] and OS [median: 17.6 versus 10.5 months, HR (95% CI): 0.56 (0.40-0.79); p < 0.001] than those with decreased Hb count (<110 g/L) in a PS-matched cohort (n = 255). For patients with normal pretreatment Hb levels, ICI combination therapy was significantly associated with better PFS [median: 11.1 versus 8.0 months, HR (95% CI): 0.74 (0.50-1.06); p = 0.09] and OS [median: 26.0 versus 12.9 months, HR (95% CI): 0.56 (0.37-0.86); p = 0.008] than monotherapy, but there was no such trend for patients with decreased baseline Hb levels. Conclusion: Our findings showed that normal pretreatment Hb count served as a favorable prognostic marker in advanced NSCLC patients treated with ICIs, representing an economical biomarker with readily measuring performance among all reported ones.

19.
Oncol Lett ; 20(6): 373, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33154771

RESUMO

Sentinel lymph nodes (SLNs) are the first lymph nodes that receive lymphatic drainage from the breast. However, all stained lymph nodes are dissected as SLNs during surgery. The present study aimed to identify and preserve the stained non-SLNs and evaluate the safety during sentinel lymph node biopsy (SLNB) in breast cancer. SLNB was performed with a methylene blue and indocyanine green double-tracer technique. The first lymph node, which was connected with lymphatic vessels from the breast, was designated as the true SLN. The lymph node that was directly connected with the output lymphatic duct of the SLN was defined as post-SLN (poSLN), whereas the stained poSLN was designated as non-SLN. Both the stained SLN and non-SLN were sent to the pathological department for definitive diagnosis. The present study demonstrated that intraoperative dissection of the lymphatic network could distinguish true SLNs and stained non-SLNs. The number of stained lymph nodes was time-dependent. Not all stained lymph nodes were real SLNs, whereas the poSLNs would be stained if the staining time interval was inappropriate. The data indicated that the poSLNs were negative for metastasis when the SLNs were negative for metastasis. Stained lymph nodes may contain non-SLNs in addition to SLNs. Resection of all stained lymph nodes is not recommended. To reduce the morbidity due to SLNB complications, the identification and preservation of stained non-SLNs during SLNB is feasible and warrants further study in the era of precision medicine.

20.
PeerJ ; 8: e10182, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33240601

RESUMO

Background: This study aimed to systematically profile the alterations and sex- and age-related differences in the drug metabolizing enzymes (DMEs) in a KRAS-mutant mouse model of lung cancer (KRAS mice). Methodology: In this study, the LC-MS/MS approach and a probe substrate method were used to detect the alterations in 21 isoforms of DMEs, as well as the enzymatic activities of five isoforms, respectively. Western blotting was applied to study the protein expression of four related receptors. Results: The proteins contents of CYP2C29 and CYP3A11, were significantly downregulated in the livers of male KRAS mice at 26 weeks (3.7- and 4.4-fold, respectively, p < 0.05). SULT1A1 and SULT1D1 were upregulated by 1.8- to 7.0- fold at 20 (p = 0.015 and 0.017, respectively) and 26 weeks (p = 0.055 and 0.031, respectively). There were positive correlations between protein expression and enzyme activity for CYP2E1, UGT1A9, SULT1A1 and SULT1D1 (r 2 ≥ 0.5, p < 0.001). Western blotting analysis revealed the downregulation of AHR, FXR and PPARα protein expression in male KRAS mice at 26 weeks. For sex-related differences, CYP2E1 was male-predominant and UGT1A2 was female-predominant in the kidney. UGT1A1 and UGT1A5 expression was female-predominant, whereas UGT2B1 exhibited male-predominant expression in liver tissue. For the tissue distribution of DMEs, 21 subtypes of DMEs were all expressed in liver tissue. In the intestine, the expression levels of CYP2C29, CYP27A1, UGT1A2, 1A5, 1A6a, 1A9, 2B1, 2B5 and 2B36 were under the limitation of quantification. The subtypes of CYP7A1, 1B1, 2E1 and UGT1A1, 2A3, 2B34 were detected in kidney tissue. Conclusions: This study, for the first time, unveils the variations and sex- and age-related differences in DMEs in C57 BL/6 (WT) mice and KRAS mice.

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