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1.
J Nanosci Nanotechnol ; 20(2): 673-679, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383062

RESUMO

A proper soft tissue seal between implants and gingiva is critical for success of dental implants. Implant surface modification is an important approach for achieving ideal host-implant integration. In this study, we used a new and simple oxidation method to generate a rough surface on implants at the nano scale, which oxidized titanium nano-foveolae (TiNF) surface. We further analyzed the surface topography and tested its effects on biological activities of human gingival fibroblasts. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) examination demonstrated that TiNF disks displayed uniform rough surfaces, with average TiNF diameters of approximately 60 nm and 100 nm respectively. However, the surfaces of smooth samples were highly irregular, and cell adhesion and proliferation rates on TiNF surfaces were significantly higher than those of the smooth surfaces. Extracellular matrix synthesis was also increased in the cells that interacted with oxidized TiNF surfaces. Altogether, these results suggest that the TiNF implant surfaces perform better for human gingival fibroblast biological activities compared to traditional smooth surfaces. Therefore, the TiNF implant surfaces may serve as ideal interface to facilitate implant-host integration.

2.
J Cataract Refract Surg ; 45(9): 1340-1345, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31470944

RESUMO

This meta-analysis studied toric intraocular lens (IOL) alignment accuracy using image-guided and manual marking methods by comparing the axis misalignment of toric IOLs, percentage of eyes with toric IOL axis misalignment within ±5 degrees, postoperative astigmatism, difference vector, and postoperative uncorrected distance visual acuity. The methodological quality was assessed using the modified Quality Assessment of Diagnostic Accuracy Studies-2 tool. Continuous variables were analyzed using weighted mean differences, and dichotomous variables were compared using the odds ratio. Five studies comprising a total of 257 eyes were analyzed. For heterogeneity, neither sensitivity analysis nor the Egger test detected statistical findings. The image-guided marking group had smaller toric IOL axis misalignment (P < .00001), less postoperative astigmatism (P = .003), and a smaller difference vector (P < .00001) than the manual marking group. The overall evidence from the studies indicates that image-guided marking is better than manual marking, resulting in less axis misalignment, a smaller difference vector, and less postoperative astigmatism.

3.
Biosci Rep ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484796

RESUMO

This study aimed to investigate whether co-administration of mesenchymal stromal cells (MSC) and linezolid (LZD) into a rabbit model of methicillin-resistant Staphylococcus aureus (MRSA)-infected pneumonia would bring a synergistic therapeutic effect. Human umbilical cord-derived MSCs (hUMSCs) were isolated and characterized. A rabbit model of pneumonia was constructed by delivering 1×1010 CFU MRSA via a bronchoscope into the basal segment of lower lobe of right lung. Through analyzing vital sign, pulmonary auscultation, SpO2, chest imaging, bronchoscopic manifestations, pathology, neutrophil percentage, and inflammatory factors, we verified that a rabbit model of MRSA-induced pneumonia was successful constructed. Individual treatment with LZD (50mg/kg for 2 times/day) resulted in improvement of body weight, chest imaging, bronchoscopic manifestations, histological parameters and IL-10 concentration in plasma ( p < 0.01), decreasing pulmonary auscultation, and reduction of IL-8, IL-6, CRP and TNF-α concentrations in plasma ( p < 0.01) compared with the pneumonia model group at 48 and 168h. Compared with LZD group, co-administration of hUMSCs (1×106/kg for 2 times at 6 and 72 h after MRSA instillation) and LZD further increased the body weight ( p < 0.05). The changes we observed from chest imaging, bronchoscopic manifestations and pathology revealed that co-administration of hUMSCs and LZD reduced lung inflammation more significantly than that of LZD group. The plasma levels of IL-8, IL-6, CRP and TNF-α in combined group decreased dramatically compared with the LZD group ( p < 0.05). In conclusion, hUMSCs administration significantly improved therapeutic effects of LZD on pneumonia resulted from MRSA infection in a rabbit model.

4.
J Ovarian Res ; 12(1): 79, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31470880

RESUMO

OBJECTIVE: To explore the risk factors for the recurrence of endometrioma and the risk factors for the recurrence of endometriosis-related pain after long-term follow-up. METHODS: This study retrospectively analyzed 358 women with endometriomas who had a minimum of 5-years follow up after laparoscopic endometrioma excision, which was performed at Peking Union Medical College Hospital from January 2009 to April 2013. All women were divided into recurrence group and nonrecurrence group. Analysis was performed with regard to preoperative history, laboratory analysis, findings during surgery, and symptoms during follow-up, including improvement and recurrence. RESULTS: The cumulative incidence rates of recurrence from 5 to 10 years after surgery were 15.4, 16.8, 19.3, 22.5, 22.5, and 22.5%, respectively. Significant differences were found between two groups in terms of age at surgery (RR: 0.764, 95% CI: 0.615-0.949, p = 0.015), duration of dysmenorrhea (RR: 1.120, 95% CI: 1.054-1.190, p < 0.001), presence of adenomyosis (RR: 1.629, 95% CI: 1.008-2.630, p = 0.046), CA125 level (RR: 1.856, 95% CI: 1.072-3.214, p = 0.021) and severity of dysmenorrhea. The severity of dysmenorrhea (RR: 1.711, 95% CI: 1.175-2.493, p = 0.005) and postoperative pregnancy (RR: 0.649, 95% CI: 0.460-0.914, p = 0.013) were significantly correlated with endometrioma recurrence in the multivariate analysis. No significant associations were found between the recurrence rate and gravida, parity, body mass index, infertility, leiomyoma presence, the size of ovarian endometrioma, the presence of deep infiltrating endometriosis, disease stage or postoperative medication. CONCLUSIONS: The severity of dysmenorrhea and postoperative pregnancy were independent risk factors for the recurrence of ovarian endometriomas after surgery during the long-time follow up.

5.
Sci Total Environ ; 697: 134092, 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31476505

RESUMO

Riparian zones are vulnerable to water regimes, which alter soil water availability and impact the persistence of riparian plants. However, little is known about the water use patterns of alpine riparian shrubs (e.g., Myricaria squamosa) in response to changes in soil water availability on the Qinghai-Tibet Plateau. This study examined the water-use patterns of M. squamosa along a zone of gradual degradation (light, moderate, and severe), located in the lower reaches of the Shaliu River in the Qinghai Lake watershed. Stable water isotopes (δ2H and δ18O) in xylem water, soil water and groundwater, as well as leaf water potential were monitored during the growing seasons from 2012 to 2013, and quantified the water-use proportions via MixSIAR model. Results showed significant differences in the isotopic signatures of M. squamosa from the light, moderate, and severe degradation sites across seasons, suggesting that M. squamosa exploited different water sources. MixSIAR results also revealed that M. squamosa used high proportions of shallow soil water in the light degradation site (35.4%) compared with the severe degradation sites (13.4%). By contrast, M. squamosa exhibited an ability to shift its water sources and to rely more on groundwater in the severe degradation site across seasons. The contrasting water-use patterns of M. squamosa along the gradual degradation zone were closely linked with the distributions of active root zones when competing for water. Higher predawn leaf water potential (Ψpd) of M. squamosa (mean Ψpd = -2.29 ±â€¯0.7 MPa) was found in the light degradation site and lower Ψpd values in the severe degradation site (mean Ψpd = -3.3 ±â€¯0.8 MPa), suggesting that M. squamosa depended on a high degree of flexible plasticity in water use to alleviate water stress along the gradual degradation. These results linked to water-use patterns and ecophysiological characteristics (e.g., Ψpd) of plants responding to changes in available water are important for informing decision-making management strategies designed to prevent ecological degradation.

6.
Small ; : e1902842, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31483576

RESUMO

Over the past several decades, lattice materials have been developed and used as engineering materials for lightweight and stiff industrial structures. Recent advances in additive manufacturing techniques have prompted the emergence of architected materials with minimum characteristic sizes ranging from several micrometers to hundreds of nanometers. Taking advantage of the topological design, structural optimization, and size effects of nanomaterials, various 3D micro-/nanolattice materials composed of different materials exhibit combinations of superior mechanical properties, such as low density, high strength (even approaching the theoretical limits), large deformability, good recoverability, and flaw tolerance. As a result, some micro-/nanolattices occupy an unprecedented area in Ashby charts with a combination of different material properties. Here, recent advances in the fabrication and mechanics of micro-/nanolattices are described. First, various design principles and advanced techniques used for the fabrication of micro-/nanolattices are summarized. Then, the mechanical behaviors and properties of micro-/nanolattices are further described, including the compressive Young's modulus, strength, energy absorption, recoverability, and tensile behavior, with an emphasis on mechanistic insights and origins. Finally, the main challenges in the fabrication and mechanics of micro-/nanolattices are addressed and an outlook for further investigations and potential applications of micro-/nanolattices in the future is provided.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31518048

RESUMO

Although great effort has been made in the past several decades, challenges still remain in the design and synthesis of uranium sorbent materials with high uptake efficiency, capacity and selectivity, as well as excellent hydrolytic stability and radiation resistance. Herein, a polyoxometalate (POM)-organic framework material ( SCU-19 ) with a rare inclined polycatenation structure was successfully designed, synthesized through solvothermal method, and tested for uranium separation for the first time. Under dark condition, SCU-19 can efficiently capture uranium through ligand complexation using its exposed oxo atoms and partial chemical reduction from U(VI) to U(IV) by the low-valent Mo atoms in the POM. Additional U(VI) photocatalytic reduction mechanism can be inserted into the system under the visible light irradiation, leading to a higher uranium removal without saturation and faster sorption kinetics. SCU-19 is currently the only uranium sorbent material with three distinct sorption mechanisms, as further demonstrated by X-ray photoelectron spectroscopy (XPS) and X-ray absorption near edge structure (XANES) analysis.

8.
Aging Cell ; : e13036, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31498540

RESUMO

Maternal age-related decline in oocyte quality is associated with meiotic defects, but the underlying mechanisms remain to be explored. Histone deacetylase 3 (HDAC3) has been shown to govern multiple cellular events via deacetylating diverse substrates. We previously found that HDAC3 could promote meiotic apparatus assembly in mouse oocytes. In the present study, we identified a substantial reduction in HDAC3 protein in oocytes from old mice. Importantly, overexpression of HDAC3 in old oocytes not only partially prevents spindle/chromosome disorganization, but also significantly lowers the incidence of aneuploidy. Meanwhile, we noticed the elevated acetylation level of α-tubulin in oocytes derived from old mice. By employing site-directed mutagenesis, we showed that acetylation-mimetic mutant tubulin-K40Q disrupts the kinetochore-microtubule attachments and results in the assembly failure of meiotic apparatus in mouse oocytes. Importantly, forced expression of tubulin-K40R (nonacetylatable-mimetic mutant) was capable of alleviating the defective phenotypes of oocytes from aged mice. To sum up, this study uncovers that loss of HDAC3 represents one potential mechanism mediating the effects of advanced maternal age on oocyte quality.

9.
Eur J Neurol ; 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31444920

RESUMO

BACKGROUND AND PURPOSE: Huntington's disease (HD) is a dominantly inherited neurodegenerative disorder with varied prevalence in different populations, which may be associated with specific haplotypes. This study aims to explore the haplotypes encompassing HTT gene in the Chinese population. METHODS: A total of 406 HD individuals and 59 normal relatives from 253 HD families were enrolled. Twenty-nine SNPs were selected and genotyped for the haplotype analysis. RESULTS: In stage one, we used 18 tag SNPs (tSNPs) to replicate the distribution of three major haplogroups (A, B, C). We found that risk-associated haplogroup variants A1 and A2, enriched on Caucasian HD chromosomes, were totally absent from both Chinese HD and control chromosomes, and the distributions of haplogroups between HD and control chromosomes were similar. Therefore, in stage two, we used 29 tSNPs (including the18 tSNPs) to define new haplogroups (I, II, III) and found haplogroup I accounting for 61.3% on HD chromosomes while 34.4% on control chromosomes, indicating haplogroup I was enriched on Chinese HD chromosomes. CONCLUSIONS: This is the first haplotype analysis encompassing HTT in the Chinese population. The results contribute to explaining the low prevalence of HD in China and provide a better understanding of genetic diversity in HTT region. This article is protected by copyright. All rights reserved.

10.
Xenobiotica ; : 1-11, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31432741

RESUMO

Bexagliflozin is a C-aryl glucoside inhibitor of human sodium-glucose linked transporter 2 (SGLT2) that undergoes oxidation and glucuronidation to form six principal metabolites in humans. In vitro metabolism by human liver microsomes and recombinant enzymes is primarily mediated by CYP3A4 and UGT1A9. Three major oxidation products and three major glucuronides have been identified in vivo. Metabolism by rats is mostly by oxidation whereas metabolism by monkeys and humans is mostly by glucuronidation. Metabolism by monkeys closely resembles metabolism by humans and all metabolites found in humans are also found in monkeys. A greater diversity of metabolites has been identified among human in vivo specimens than among in vitro reaction products. Following oral dosing of humans with 14C-bexagliflozin, the 3'-O-glucuronide contributed 32% of the parent AUC and all other metabolites contributed <10%. Of the 91.6% of input radioactivity recovered, 51.1% was in faeces, predominantly as bexagliflozin, and 40.5% was in urine, largely as the 3'-O-glucuronide. Unidentified metabolites contributed 0.27% of the input radiolabel. A quantitative accounting for the metabolism and disposition of bexagliflozin in vivo has been developed.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31451414

RESUMO

BACKGROUND: In order to better understand the role of Dendritic cells (DCs) in Chronic Hepatitis B (CHB), we investigated the frequencies and maturation markers on DCs in CHB patients and its change during entecavir treatment. METHODS: Twenty-six CHB patients on anti-virus treatment for 48 weeks were included in this study. Patients' blood samples were collected on every 3 months since starting treatment. Samples on baseline and after 48 weeks treatment were examined using flow-cytometry to investigate frequencies and maturation markers of DCs. RESULTS: The frequencies of myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) were lower in CHB patients than healthy controls on baseline. pDCs frequencies and mDCs maturation markers expression were increased after entecavir (ETV) treatment. Patients with higher baseline HBsAg levels showed a poorer maturation status than those with low baseline HBsAg levels, regardless of changes in HBsAg levels after treatment. CONCLUSIONS: Entecavir treatment could restore the decreased DCs frequencies in CHB patients and improve DCs maturation levels. Baseline HBsAg level is an important factor that affecting DCs.

12.
ACS Appl Mater Interfaces ; 11(35): 32192-32199, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31442031

RESUMO

Thermoelectric materials are of imperative need on account of the worldwide energy crisis. However, their efficiency is limited by the interplay of high electrical and lower thermal conductivities, that is, the figure of merit (ZT). Owing to their unique crystal structures, Cu-In-Te-based chalcogenides are suitable for both and thus have attracted much attention recently as potential thermoelectrics. Here we explore a newly developed Cu-In-Te derivative compound Cu3.52In4.16Te8. With a proper adjustment of Cu2Te doping, this material shows an ultralow lattice thermal conductivity (κL) (0.3 WK-1m-1) and, consequently, a figure of merit (ZT) as high as 1.65(±0.15) at 815 K: the highest value reported for p-type Cu-In-Te to date. The reduction in κL is directly related to the alteration of local symmetry around the interstitial Te, resulting in an effectively optimized phonon transport through localized "rattling" of the same. Although the Hall carrier concentration reduces upon Cu2Te addition due to the unpinning of the Fermi level (EFermi) toward the conduction band minimum, the power factor remains stable. The knowledge depicted here not only demonstrates the potential of Cu3.52In4.16Te8-based alloys as a promising TE, but also provides guidelines for developing further high-performance thermoelectric materials by enhancing the electronic conductivity.

13.
Biochem Biophys Res Commun ; 518(2): 278-285, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31420161

RESUMO

Uncontrollable vascular smooth cell proliferation is responsible for vascular remodeling during hypertension development. Glyoxalase 1 (GLO1), the major enzyme detoxifying methylglyoxal, has a critical role in regulating proliferation of several cell types. However, little is known whether GLO1 is involved in cerebrovascular remodeling and basilar smooth muscle cell (BASMC) proliferation during hypertension. Here we explored the role of GLO1 in angiotensin II (Ang II)-induced cerebrovascular remodeling and proliferation of BASMCs and the underlying mechanisms. The protein expression of GLO1 in basilar arteries from hypertensive mice was decreased, and GLO1 expression was negatively correlated with medial cross-sectional area and blood pressure in basilar arteries during hypertension. Knockdown of GLO1 promoted while overexpression of GLO1 prevented Ang II-induced cell proliferation and cell cycle transition in BASMCs. These results were related to the inhibitory effects of GLO1 on PI3K/AKT/CDK2 cascade activation upon Ang II treatment. In addition, in vivo study, GLO1 overexpression with adeno-associated virus harboring GLO1 cDNA improved cerebrovascular remodeling in basilar artery tissue during Ang II-induced hypertension development. These data indicate that GLO1 reduction mediates cerebrovascular modeling via PI3K/AKT/CDK2 cascade-dependent BASMC proliferation. GLO1 acts as a negative regulator of hypertension-induced cerebrovascular remodeling and targeting GLO1 may be a novel therapeutic strategy to prevent hypertension-associated cardiovascular complications such as stroke.

14.
Free Radic Biol Med ; 143: 288-299, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31445205

RESUMO

Transient Receptor Potential Melastatin-2 (TRPM2) is a nonselective cation channel mediating Ca2+ influx in response to oxidative stress. Given that insulin resistance-related endothelial dysfunction in obesity attributes to fatty-acid-induced reactive oxygen species (ROS) overproduction, in this study, we addressed the possible role of TRPM2 in obesity-related endothelial insulin resistance and the underlying mechanisms. Whole-cell patch clamp technique, intracellular Ca2+ concentration measurement, western blot, vasorelaxation assay, and high-fat diet (HFD)-induced obese model were employed to assess the relationship between TRPM2 and endothelial insulin response. We found that both the expression and activity of TRPM2 were higher in endothelial cells of obese mice. Palmitate rose a cationic current in endothelial cells which was inhibited or enlarged by TRPM2 knockdown or overexpression. Silencing of TRPM2 remarkably improved insulin-induced endothelial Akt activation, nitric oxide synthase (eNOS) phosphorylation and nitric oxide (NO) production, while TRPM2 overexpression resulted in the opposite effects. Furthermore, TRPM2-mediated Ca2+ entry, CaMKII activation and the following activation of PERK/ATF4/TRB3 cascade were involved in the mechanism of obesity or palmitate-induced endothelial insulin resistance. Notably, in vivo study, knockdown of TRPM2 with adeno-associated virus harboring short-hairpin RNA (shRNA) against TRPM2 alleviated endothelial insulin resistance and ameliorated endothelium-dependent vasodilatation in obese mice. Thus, these results suggest that TRPM2-activated Ca2+ signaling is necessary to induce insulin resistance-related endothelial dysfunction in obesity. Downregulation or pharmacological inhibition of TRPM2 channels may lead to the development of effective drugs for treatment of endothelial dysfunction associated with oxidative stress state.

15.
Zhongguo Fei Ai Za Zhi ; 22(8): 488-493, 2019 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-31451138

RESUMO

BACKGROUND: Anaplastic lymphoma kinase (ALK) positive in non-small cell lung cancer (NSCLC) was about 5%-7% and ALK tyrosine kinase inhibitor (TKI) was the standard treatment in NSCLC. The aim of this study is to evaluate the efficacy and safety of crizotinib in patients with advanced ALK gene-positive or recurrent NSCLC. METHODS: Three methods were used to screen patients with advanced or recurrent NSCLC harboring ALK gene fusion/translocation. The patients with ALK positive tested by flourescence in situ hybridization (FISH) was given orally crizotinib, 250 mg, bid. The objective response rate (ORR), progression-free survival (PFS) and safety were evaluated. RESULTS: A total of 226 patients were screened, 39 of whom had ALK fusion or translocation, and 37 were enrolled in the study. 35 patients were evaluated for objective response, ORR was 70.3%, and disease control rate (DCR) was 94.6%, and median PFS was 11.8 mon. The main adverse reactions were elevated transaminase (Grade 1, 91.7%), elevated transaminases (Grade 2, 23.4%), nausea (Grade 1, 75.6%), anemia (Grade 1-2, 62.3%), visual impairment (Grade 1, 21.8%), weight loss (Grade 1, 31.4%), pneumonia (Grade 2, 3.5%). CONCLUSIONS: Crizotinib can be used for the treatment of advanced NSCLC with ALK fusion/translocation. It is highly effective and well tolerated.

16.
Zhongguo Fei Ai Za Zhi ; 22(8): 507-511, 2019 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-31451141

RESUMO

BACKGROUND: Non-small cell lung cancer (NSCLC) with neuroendocrine differentiation (NED) was a new pathologic type and uncommon in clinics. The aim of this study is to observe the relationship between clinical pathologic characteristics, imagination, biological behavior and prognosis in NSCLC-NED. METHODS: The clinical data of 47 patients with NSCLC-NED admitted from January 2009 to November 2017 in the Fifth Medical Center of General Hospital of People's Liberation Army were collected. The demographic data and imaging characteristics were summarized. Pathological features, treatment and prognosis, analysis of the correlation between different factors and prognosis. RESULTS: Of the 47 patients with NSCLC-NED, the median age was 61 years (45 years-78 years), 38 males and 9 females; 37 were poorly differentiated cancer with NED, and 10 were middle differentiated cancer with NED; 2 cases of driving gene positive (1 case of EGFR sensitive mutation, 1 case of ALK fusion), objective response rate (ORR) of first-line chemotherapy was 34.5%, and median progression-free survival (PFS) was 4 months; the median overall survival (OS) was 11 months, and only 2 cases (4.2%, 2/47) of OS were over 2 years. CONCLUSIONS: NSCLC-NED is different from simple NSCLC or pulmonary neuroendocrine tumors. Males, ≤70 years old, severely smoking, and patients with lower tumor differentiation often have NED, and most of them are stage IV. This type of patient-driven gene positive proportion is lower than the general adenocarcinoma population, less sensitive to chemotherapy, and the overall survival is shorter, indicating a poor prognosis.

17.
Rev Assoc Med Bras (1992) ; 65(7): 971-976, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31389507

RESUMO

OBJECTIVE: To investigate the relations of T lymphocytes, cytokines, immunoglobulin E, and nitric oxide with otitis media with effusion (OME) in children and their clinical significances. METHODS: Fifty children with OME treated in our hospital were enrolled in the study (observation group). Fifty healthy children were selected as control. The percentages of CD4+ and CD8+ T lymphocyte and CD4+/CD8+ ratio in peripheral blood, and the levels of cytokine (IL)-2, IL-4, IL-6, immunoglobulin E (IgE) and nitric oxide (NO) in peripheral blood and middle ear effusion (MEE) in both groups were detected. The correlations of these indexes with OME were analyzed. RESULTS: The percentage of peripheral blood CD4+ and CD8+ levels, CD4+/CD8 ratio, IgE, and NO levels in the observation group were significantly higher than those in the control group (P < 0.01). In the observation group, the IL-2 and IL-6 levels, and IgE and NO levels in the MEE were significantly higher than those in peripheral blood (P < 0.01). In addition, in the observation group, the MEE IL-2 and IL-6 levels were positively correlated with peripheral blood CD4+/CD8+ ratio, respectively r = 0.366, P = 0.009; r = 0.334, P = 0.018. CONCLUSIONS: The levels of peripheral blood CD4+ and CD8+ lymphocytes and MEE IL-2, IL-6, IgE, and NO levels are increased in children with OME. These indexes have provided significant clues for the diagnosis of OME in children.


Assuntos
Linfócitos T CD4-Positivos , Linfócitos T CD8-Positivos , Citocinas/sangue , Imunoglobulina E/sangue , Óxido Nítrico/sangue , Otite Média com Derrame/sangue , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Citometria de Fluxo , Humanos , Contagem de Linfócitos , Masculino , Valores de Referência , Membrana Timpânica/metabolismo
18.
Oncogene ; 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31406252

RESUMO

Increasing evidence has indicated that circular RNAs (circRNAs) play a critical role in cancer development. However, only a small number of circRNAs have been experimentally validated and functionally annotated. In this study, using a high-throughput microarray assay, we identified a novel circRNA, circKDM4C, which was downregulated in breast cancer tissues with metastasis. Furthermore, we analyzed a cohort of breast cancer patients and found that circKDM4C expression was decreased in breast cancer tissues, and lower circKDM4C expression was associated with poor prognosis and metastasis in breast cancer. Functionally, we demonstrated that circKDM4C significantly repressed breast cancer proliferation, metastasis, and doxorubicin resistance in vitro and in vivo. Mechanistically, using a dual-luciferase activity assay and AGO2 RNA immunoprecipitation, circKDM4C was identified as a miR-548p sponge. We also found that PBLD was a direct target of miR-548p, which functioned as a tumor suppressor in breast cancer. Moreover, miR-548p overexpression was able to reverse the circKDM4C-induced attenuation of malignant phenotypes and elevated expression of PBLD in breast cancer cells. Taken together, our data indicate that circKDM4C might have considerable potential as a prognostic biomarker in breast cancer, and support the notion that therapeutic targeting of circKDM4C/miR-548p/PBLD axis may be a promising treatment approach for breast cancer patients.

19.
J BUON ; 24(3): 1276-1282, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31424690

RESUMO

PURPOSE: Adenoid cystic carcinoma is a rare and under-researched disease. There is hardly any chemotherapy available for it, hence the urgent need to develop novel and efficient chemotherapy. Therefore, we examined the anticancer effects of globularifolin, an acylated iridoid glucoside, against salivary adenoid cystic carcinoma (SACC-83) cell line and normal human salivary gland (HSG) cell line. METHODS: Cell counting and colony formation assays were used to determine cell viability. Acridin orange (AO)/ethidium bromide (EB) staining and comet assay were used for the detection of apoptosis. Reactive oxygen species (ROS) determination and cell cycle analysis were performed by flow cytometry. Transwell assay was used to monitor cell migration and Western blot analysis was used to determine protein expression. RESULTS: Globularifolin inhibited the growth of SACC-83 cell line and exhibited an IC50 of 10 µM. Nonetheless, the cytotoxic effects of globularifolin were comparatively negligible against normal HGS cells with an IC50 of 80 µM. The investigation of the mechanism of action revealed that the anticancer effects of globularifolin against the SACC-83 cells was due to the induction of apoptotic cell death as indicated by AO/EB staining. Globularifolin treatment also resulted in enhancement of the Bax, Caspase 3 and 9 expression and decline of the Bcl-2 expression. Globularifolin also blocked the SACC-83 cells at the G0/G1 phase of the cell cycle. Moreover, cell invasion assay revealed that globularifolin inhibited the migration of the SACC-83 cells concentration-dependently, which was also coupled with the downregulation of metalloproteinase (MMP) 2 and 9. JAK/STAT is an important pathway involved in the proliferation and tumorigenesis of cancer cells and this research found that globularifolin could inhibit this pathway. CONCLUSION: We conclude that globularifolin may prove essential in the development of systemic therapy for adenoid cystic carcinoma.

20.
Food Sci Technol Int ; : 1082013219867190, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31399018

RESUMO

The aim of this study was to statistically evaluate the effect of a naturally food-derived cinnamaldehyde on the thermal inactivation of Listeria monocytogenes in ground pork. This study combined four concentrations of cinnamaldehyde (0, 0.1, 0.5, and 1.0% vol/wt) and four temperatures (55, 60, 65, and 70 ℃) to predict the thermal inactivation curves of L. monocytogenes. The Weibull model successfully described the primary thermal inactivation using the Integrated Pathogen Modeling Program. These results statistically proposed that the cinnamaldehyde supplementation in ground pork attenuates the thermo-tolerance of L. monocytogenes. The time for achieving a 5-log10 reduction of L. monocytogenes declined from 28.14 to 17.35 min at 55 ℃ when the ground pork sample was supplemented by 1% cinnamaldehyde, while the time declined from 1.95 to 0.34 min at 70 ℃. Thereafter, based on the 5.0-log10 lethality, secondary models were fitted by a selected polynomial model. The transmission electron microscopy revealed that cinnamaldehyde causes serious damage to membrane integrity and increases the occurrence of cell membrane rupture and leakage of cytoplasmic content under thermal treatment. Our model represents a mathematical tool that will help meat-product manufacturers to improve the efficacy of thermal processing ground pork supplemented with cinnamaldehyde.

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