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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117428, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31376727

RESUMO

Room temperature phosphorescence (RTP) materials have become a hot topic in fields of organic light-emitting dioes, biological sensing and imaging. The present work reports firstly that 1,3,5-trifluoro-2,4,6-triiodobenzene (TITFB) can act as a simple pure organic NIR phosphor due to its novel function in promoting n-π∗ transition. Also, TITFB crystal has longer phosphorescence lifetime than other ordinary multiiodoluminophors and TITFB powder. Based on the TITFB crystal structure, σ-hole and π-hole capture mechanism of n-electron is proposed, i.e., the excited state energy is decreased and n-electrons are stabilized to cause slower radiative decay rate due to the restriction of σ-hole and π-hole bond. Both computational and experimental studies support the mechanism. The new electron-capture mode is more conducive to understanding pure organic ultralong lifetime RTP.

2.
Chemosphere ; 238: 124602, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31545211

RESUMO

Polybrominated diphenyl ethers (PBDEs) have been known to exhibit neurotoxicity in rats; however, the underlying mechanism remains unknown and there is no available intervention. In this study, we aimed to investigate the role of oxidative and nitrosative stress in the neurotoxicity in the cerebral cortex and primary neurons in rats following the BDE-153 treatment. Compared to the untreated group, BDE-153 treatment significantly induced the neurotoxic effects in rats, as manifested by the increased lactate dehydrogenase (LDH) activities and cell apoptosis rates, and the decreased neurotrophic factor contents and cholinergic enzyme activities in rats' cerebral cortices and primary neurons. When compared to the untreated group, the oxidative and nitrosative stress had occurred in the cerebral cortex or primary neurons in rats following the BDE-153 treatment, as manifested by the increments in levels of reactive oxygenspecies (ROS), malondialdehyde (MDA), nitric oxide (NO), and neuronal nitric oxide synthase (nNOS) mRNA and protein expressions, along with the decline in levels of superoxide dismutase (SOD) activity, glutathione (GSH) content, and peroxiredoxin I (Prx I) and Prx II mRNA and protein expressions. In addition, the ROS scavenger N-acetyl-l-cysteine (NAC) or NO scavenger NG-Nitro-l-arginine (L-NNA) significantly rescued the LDH leakage and cell survival, reversed the neurotrophin contents and cholinergic enzymes, mainly via regaining balance between oxidation/nitrosation and antioxidation. Overall, our findings suggested that oxidative and nitrosative stresses are involved in the neurotoxicity induced by BDE-153, and that the antioxidation is a potential targeted intervention.

3.
J Nanosci Nanotechnol ; 20(4): 2205-2213, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492229

RESUMO

In this study, three-dimensional (3D) networked porous polyvinyl alcohol/sodium alginate/graphene oxide (PVA/SA/GO) spherical composites were fabricated by the sol-gel method and employed as adsorbents for the adsorption of methylene blue (MB) in aqueous solution. The obtained samples were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal property analysis, and nitrogen adsorption/desorption isotherms. Moreover, the adsorption properties for MB were investigated by batch experiments. The pseudo-first-order and pseudo-second-order equations were used to fit the adsorption kinetics data, and the Langmuir and Freundlich isothermal models were used to analyze the adsorption isothermals. The results showed that the spherical composites had 3D porous structures, and GO, PVA and SA were fused and linked together by self-assembly, physical intertwining, hydrogen bonding, and Ca2+ and boric acid crosslinking. The maximum adsorption capacity of the 3D porous PVA/SA/GO spherical composites for MB was 759.3 mg/g. The adsorption kinetics had a better agreement with the pseudo-secondorder equation than the pseudo-first-order equation, and the equilibrium data followed the Freundlich model.

5.
J Nanosci Nanotechnol ; 20(1): 447-451, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383192

RESUMO

The electrical and optical properties of micro-light emitting diodes (µ-LEDs), including current-voltage (I-V), capacitance-voltage (C-V) curves, photoluminescence (PL) as well as electroluminescence (EL) spectra have been measured and analyzed. It is found that the unit area emitting intensity of small size µ-LED is stronger that of big size µ-LED at the same conditions, due to the enhancement of both the internal quantum efficiency ηint and extraction efficiency Cex. The present method of utilizing the µ-LED for improving the unit area brightness of LEDs is applicable to high efficiency surface emitting device on GaN-on-silicon platform.

6.
J Opt Soc Am A Opt Image Sci Vis ; 36(10): 1795-1800, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674446

RESUMO

To solve the imaging distortion problem in a nonuniform temperature field for visual measurement, this paper studies the Runge-Kutta ray tracing algorithm. First, the light ray emitted by the spatial light point will be abstracted by the proposed method. Then, the ray trajectory in a 3D nonuniform temperature gradient environment will be reproduced, and the ray distortion can be corrected. Finally, the distortion is compared with the actual image distortion obtained through the corner detection method. The experimental results show that the correction method for the distortion image caused by nonuniform temperature gradient heat source interference is feasible by using the Runge-Kutta ray tracing algorithm, which can realize the error compensation for image distortion and obtain an accurate corrected image.

7.
Pharmacol Res ; : 104510, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31678209

RESUMO

Herbal medicines are widely used as alternative or complementary therapies worldwide to treat and prevent chronic diseases. However, herbal medicines coadministration with therapeutic drugs may cause dramatic clinical herb-drug/herb interactions (HDIs/HHIs) that may result in low drug efficacy or serious toxic reactions. Phase II metabolism enzyme UDP-glucuronosyltransferases (UGTs) play a significant detoxification role in vivo. Most drugs and non-drug xenobiotics undergo phase II metabolic transformations to be more polar compounds that are more easily excreted. Herbal medicines are a mixed and chemically varied group that includes flavonoids, stilbenes, coumarins, quinones, and terpenes, which are potential substrates and inhibitors of UGTs. Although increasing studies about glucuronidation metabolism and the inhibition toward UGTs of many herbal medicines have been reported, it is still difficult to determine which compounds from herbal medicines are substrates or inhibitors of UGTs. This article gives an overview of UGTs studies, which mainly focuses on glucuronidation of herbal constituents as substrates catalyzed by UGTs, potential herbal inhibitors for UGTs. We summarize the negative effects of UGT1A polymorphism and single nucleotide polymorphisms (SNPs), relevant clinical situations of HDIs/HHIs induced by inhibition of UGTs, and propose establishing classification criteria for inhibitors. Finally, we also discuss future research and strategic directions to advance the understanding of the potential HDIs/HHIs and suggest some additional studies revealing more information on UGT-mediated HDIs/HHIs.

8.
J Colloid Interface Sci ; 560: 293-302, 2019 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-31670102

RESUMO

Hierarchical copper sulfide/bismuth tungstate (CuS/Bi2WO6) p-n junction with two-dimensional (2D) interfacial coupling were prepared through a two-step hydrothermal method. The as-prepared samples were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, diffuse reflectance spectroscopy, photoluminescence spectroscopy, photocurrent and electrochemical impedance spectroscopy. CuS nanoplates anchored on the surface of hierarchical Bi2WO6 microspheres consisting of Bi2WO6 nanosheets to form a CuS/Bi2WO6 p-n junction photocatalyst. The photocatalytic activity of glyphosate degradation over CuS/Bi2WO6 under 44 W light-emitting diode (LED) light irradiation (λ > 400 nm) was investigated. The results indicate that the CuS/Bi2WO6 heterojunctions display higher photocatalytic activity than pure CuS and Bi2WO6. The improved photocatalytic activity of the CuS/Bi2WO6 heterostructures can be attributed to strong visible light absorption and enhanced separation of photogenerated charge carriers by the internal electric field near the interface of the p-n junction. In addition, the CuS/Bi2WO6 p-n junction photocatalysts consisting of nanosheets are beneficial for the transport of photoexcited charge carriers, thus resulting in the increase of the photocatalytic activity of glyphosate degradation. This work provides the new avenue for the development and design p-n junction materials for photocatalytic applications.

9.
Opt Express ; 27(22): 32649-32658, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684473

RESUMO

We propose and experimentally demonstrate an ytterbium-doped fiber laser emitting the single high-order cylindrical vector beams with a high efficiency and a high modal purity based on adaptive modal gain control. By the combination of a high-order pump with a self-designed ytterbium-ring doped fiber, modal dependent gain was tailored and specific transverse mode can be selected in the laser cavity. A model based on multimode propagation-rate equations is built up to demonstrate the behaviors of transverse mode competition in the fiber laser. Modal dependent gain of high-order mode pump are simulated numerically, which agree well with our experiment results. The slope efficiency of the fiber laser reaches 79.61% with a low threshold of 47.73mw. The purity of the generated high-order CVBs are in excess of 95%. Such a strategy enables the controllability of modal gain in a fiber laser and reveals the potential to offer a new and promising way to achieve a high-power fiber laser with an arbitrary single high-order transverse modes output.

10.
J Med Econ ; : 1, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31686551

RESUMO

Purpose: This study evaluated healthcare resource utilization (HCRU), and direct costs among severe aplastic anemia (SAA) patients treated with eltrombopag (EPAG) using US claims data.Methods: This retrospective, real-world claims database study identified SAA patients aged ≥2 years treated with EPAG who initiated any SAA treatment between July 1, 2014 and December 31, 2017 (identification period) using the Truven MarketScan® databases. A subset of 82 patients treated with EPAG during the identification period were evaluated for all-cause and SAA-related HCRU and direct costs as well as blood transfusion one month before EPAG initiation (baseline) and at Month 6 after EPAG initiation (follow-up period).Results: The average patient age was 50.8 (SD =20.6) years old, predominantly female (n = 43, 52.4%), and had a mean CCI at baseline of 1.1 (SD =1.7). Hospitalizations, and ER, office, and outpatient visits were significantly lower at Month 6 after EPAG initiation compared with one month before EPAG initiation (p < 0.05 for all four all-cause HCRU and SAA-related hospitalizations). An almost 2-fold decrease in reliance on biweekly blood transfusions was observed: 1.0 at weeks 1-2 to 0.5 at Month 6 after EPAG initiation. Although prescription costs (mean [SD]) were significantly higher at Month 6 after EPAG initiation compared with one month before EPAG initiation (difference of $11,045 USD [SD=$18,801]), these increases were offset by savings in direct costs. Overall, a mean reduction in total all-cause costs of $29,391 USD [SD=$137,770] was reported at Month 6 after EPAG initiation due to substantial reductions in hospitalization ($40,060 USD [SD=$123,198]) and outpatient visits ($2,043 USD [SD=$25,264]).Conclusion: All-cause and SAA-related HCRU were reduced following EPAG treatment. Prescription costs were higher following treatment; however, these costs were generally offset by reductions in direct costs. These results provide real-world evidence around the role of EPAG in SAA treatment.

11.
Plast Reconstr Surg ; 144(5): 1057-1065, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31688750

RESUMO

BACKGROUND: Aesthetic facial contouring procedures, particularly forehead augmentation, continue to increase in popularity in the Asian population. However, there are very few reports in the literature specifically examining the long-term outcomes of forehead fat grafting. In the present study, the authors aimed to objectively evaluate the efficacy of autologous forehead fat grafting through evaluation of facial proportions and patient-reported outcomes. METHODS: Patients who underwent autologous fat grafting for forehead contouring procedures between July of 2011 and June of 2017 were recruited for participation in the study. Patient demographic and clinical variables were collected preoperatively and postoperatively. Facial proportion analysis was collected and compared from preoperative and postoperative photographs. Satisfaction with outcome was assessed by the patient, the surgeon, and a layperson postoperatively. A related-samples Wilcoxon signed rank test was used for statistical analysis. RESULTS: Postoperative outcomes were evaluated in a total of 24 patients who underwent forehead autologous fat-grafting procedures. The average postoperative follow-up was 8.1 months. There were no intraoperative or postoperative complications. More than 50 percent of the postoperative results were evaluated as "satisfactory" by all three groups (i.e., patient, surgeon, and layperson) in terms of aesthetic contouring. Average forehead projection increased 0.24 U (ratio of horizontal distance from midforehead plane to cornea plane/cornea diameter) after one round of fat grafting (p = 0.01). Seven patients (29.2 percent) required more than a single round of fat grafting to achieve optimal results. CONCLUSIONS: Autologous fat grafting remains a viable option for forehead contouring and augmentation. The findings of this study demonstrate that the operation is effective and offers stable satisfactory results after long-term follow-up. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, IV.

12.
Eur Radiol ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31691121

RESUMO

OBJECTIVE: To evaluate the impact of utilizing digital breast tomosynthesis (DBT) or/and full-field digital mammography (FFDM), and different transfer learning strategies on deep convolutional neural network (DCNN)-based mass classification for breast cancer. METHODS: We retrospectively collected 441 patients with both DBT and FFDM on which regions of interest (ROIs) covering the malignant, benign and normal tissues were extracted for DCNN training and validation. Experiments were conducted for tasks in distinguishing malignant/benign/normal: (1) classification capabilities of DBT vs FFDM and the role of transfer learning were validated on 2D-DCNN; (2) different strategies of combining DBT and FFDM and the associated impacts on classification were explored; (3) 2D-DCNN and 3D-DCNN trained from scratch with volumetric DBT were compared. RESULTS: 2D-DCNN with transfer learning outperformed that without for DBT in distinguishing malignant (ΔAUC = 0.059 ± 0.009, p < 0.001), benign (ΔAUC = 0.095 ± 0.010, p < 0.001) and normal tissue (ΔAUC = 0.042 ± 0.004, p < 0.001) (paired samples t test). 2D-DCNN trained on DBT (with transfer learning) achieved higher accuracy than those on FFDM (malignant: ΔAUC = 0.014 ± 0.014, p = 0.037; benign: ΔAUC = 0.031 ± 0.006, p < 0.001; normal: ΔAUC = 0.017 ± 0.004, p < 0.001) (independent samples t test). The 2D-DCNN employing both DBT and FFDM for training achieved better performances in benign (FFDM: ΔAUC = 0.010 ± 0.008, p < 0.001; DBT: ΔAUC = 0.009 ± 0.005, p < 0.001) and normal (FFDM: ΔAUC = 0.005 ± 0.003, p < 0.001; DBT: ΔAUC = 0.002 ± 0.002, p < 0.001) (related samples Friedman test). The 3D-DCNN and 2D-DCNN trained from scratch with DBT only produced moderate classification. CONCLUSIONS: Transfer learning facilitates mass classification for both DBT and FFDM, and DBT outperforms FFDM when equipped with transfer learning. Integrating DBT and FFDM in DCNN training enhances mass classification accuracy for breast cancer. KEY POINTS: • Transfer learning facilitates mass classification for both DBT and FFDM, and the DBT-based DCNN outperforms the FFDM-based DCNN when equipped with transfer learning. • Integrating DBT and FFDM in DCNN training enhances breast mass classification accuracy. • 3D-DCNN/2D-DCNN trained from scratch with volumetric DBT but without transfer learning only produce moderate mass classification result.

13.
Neurotox Res ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31691188

RESUMO

Cognitive deficits and psychiatric disorders have been regarded as the most common clinical symptoms of methamphetamine (MA) users. Accumulating evidence has shown that liver disease may be involved in cognitive deficits and psychiatric disorders. This study examines whether cognitive deficits and psychiatric symptoms are associated with serum levels of liver biomarkers in MA users. Cognition was assessed by the Repeatable Battery for the Assessment of neuropsychological Status (RBANS). Psychiatric symptoms were assessed by the Symptom Checklist-90 (SCL-90). Liver function was assessed by serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, albumin, globulin, Apolipoprotein B (ApoB), triglyceride, total cholesterol, and glucose concentrations in 106 MA addicts and 76 controls. Compared to control subjects, MA users had greater severity of psychotic symptoms on the dimension of somatization, depression, anxiety, psychoticism, addiction, and global severity index in SCL-90, and lower scores of cognition, including the total RBANS score and all five subscales. The globulin levels were increased, while the albumin, albumin/globulin, and ApoB levels were decreased. ApoB levels were positively correlated with immediate memory, attention, and total RBANS score. Furthermore, stepwise multivariate regression analysis indicated that ApoB levels were associated with immediate memory, attention, and total RBANS score. The findings of this study suggest that MA addicts might experience cognitive deficits, psychiatric disorders, and liver damage. Serum ApoB levels may be involved in cognitive deficits; thus, improving liver function may help to treat cognitive deficits and psychiatric disorders in MA addicts.

14.
Australas J Dermatol ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31691949

RESUMO

Erythema papulatum centrifugum (EPC), also known as erythema papulosa semicircularis recidivans (EPSR), is distinct from eczema and other well-described figurate erythemas characterised by annular erythematous lesions. We report 7 cases of EPC and propose new diagnostic criteria including the following: (i) EPC is characterised by single or multiple recurrent expanding annular or semi annular erythema with central regression, surrounded by tiny red papules; (ii) the lesions regularly relapse and resolve; (iii) the histopathologic feature shows superficial perivascular inflammation with or without mild inflammation around sweat glands in the mid dermis and (iv) patients lack other associated cutaneous or internal abnormalities.

15.
Environ Monit Assess ; 191(12): 709, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31677005

RESUMO

With the increase of population, many cities are growing in size at a phenomenal rate. Urbanization changes the urban underlying surface, influences the micro-climate, and sometimes affects the local precipitation process. In this study, we investigated the trends of extreme rainfall in China's 21 typical urban areas. Based on a series of daily rainfall and "Urban/built-up" dataset from TMPA 3B42 and MCD12Q1 products in China, trends in extreme precipitation, with the threshold defined as 95th (pre95p) and 99th (pre99p) percentiles of annual rain days during 1998-2015, have been assessed in China, and especially in 21 typical urban areas from 1998 to 2015. The tendency curves in extreme rainfall of different years are presented. In this period, more than 66% regions of China covered by TMPA 3B42 have increasing trends in extreme rainfall with pre95p threshold. The 21 typical urban areas showed different trends-in over half of these areas, upward tendencies in extreme rainfall were observed, particularly in Dalian, Beijing, and Chongqing. Seventeen urban areas showed increasing tendencies in pre95p extreme rainfall days, including Shanghai, Nanjing, Hangzhou, and Suzhou in the Yangtze River Delta region. The results also illustrate that southeastern coastal urban areas of China may have experienced decreasing occurrences in extreme rainfall.

16.
Infection ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31677085

RESUMO

BACKGROUND: Inappropriate initial antimicrobial therapy (IIAT) may increase the mortality rate of hematological malignancies (HMs) patients with Gram-negative bacteria bloodstream infections (GN-BSI). The aim of this study is to determine whether IIAT affects the prognosis in this patient population and recommend the appropriate antibiotic regimen to minimize IIAT. METHODS: We reviewed a retrospective cohort study of 361 HM patients with neutropenic fever from GN-BSI. The patients' clinical characteristics and the results of the drug sensitivity test in vitro were analyzed. RESULTS: IIAT rate was 21.3% in HM patients with neutropenic fever caused by GN-BSI. There was a significant difference in 7-day mortality rate between patients treated with appropriate antibiotics and those with IIAT (7.7% vs 29.9%, p < 0.01). Multivariate analysis confirmed that IIAT was an independent risk factors for early mortality [4.860 (1.541-15.323)]. Drug sensitivity data of GN-bacteria suggested that carbapenems monotherapy or beta-lactamase inhibitors (BLBLI) combined with amikacin as the initial therapy can effectively reduce the IIAT rate. In the stratified antibiogram based on prior antimicrobial exposure, our results showed that BLBLI monotherapy could be initially used as an empirical treatment in patients without prior antimicrobial exposure. In those who had received prior antimicrobial exposure, BLBLI (especially piperacillin-tazobactam) combined with amikacin is recommended. CONCLUSIONS: IIAT was a critical factor contributing to the mortality of HM patients with neutropenic fever from GN-BSI.

17.
J Periodontal Res ; 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31677272

RESUMO

BACKGROUND AND OBJECTIVES: Peri-implantitis remains a challenge for dental implant therapy, and the prognosis of non-surgical therapy for peri-implantitis is unsatisfactory. In order to reveal the impact of non-surgical mechanical debridement therapy on microbial communities, we investigated the subgingival microbial communities of healthy implants and implants with peri-implantitis, both before and after the therapy. MATERIAL AND METHODS: Subgingival plaques were collected from patients with healthy dental implants (HC; n = 10) and from patients with peri-implantitis (n = 13) before and after non-surgical mechanical debridement therapy. The treatment was conducted using curettes for submucosal debridement followed by irrigation with 0.2% (w/v) chlorhexidine, with re-examination 1 month later. 16S rRNA pyrosequencing was used to analyze the subgingival microbiome, and co-occurrence networks were adopted to explore the interactions between pathogens in the microbial communities. RESULTS: A total of 506 955 high-quality reads were generated, and 2222 operational taxonomic units were finally detected using a 97% similarity cutoff, with a mean of 249 ± 69 per sample. The peri-implantitis sites harbored similar microbial communities before and after the treatment, as demonstrated by the microbial diversity, relative abundance, and prevalence of bacteria. Most importantly, the microbial community structures were stable before and after non-surgical therapy based on the microbial diversity and bacterial composition, as well as the interactions between key pathogens, including Enterobacteriaceae, Selenomonas sputigena, Parvimonas, Eubacterium infirmum, Campylobacter gracilis, Tannerella forsythia, and Fusobacterium, which were measured using a co-occurrence network analysis. Periodontal pathogens were also detected in subgingival plaque after the treatment. Distinct microbial communities were found between the healthy and peri-implantitis sites. CONCLUSION: Our results demonstrate that non-surgical mechanical debridement therapy did not significantly affect the subgingival microbial communities in peri-implantitis, and the stable microbial networks created via interactions among pathogens may be responsible for the poor prognosis of peri-implantitis treatment.

18.
Bioorg Chem ; : 103385, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31669094

RESUMO

A series of homoerythrina alkaloid derivatives containing a 1,2,3-triazole moiety as PARP-1 inhibitors were designed and synthesized. And their anti-proliferative activity was further evaluated. Compound 10n had excellent activity to inhibit proliferation of A549 cells (IC50 = 1.89 µM), which was higher than harringtonine (IC50 = 10.55 µM), pemetrexed (IC50 = 3.39 µM), and rucaparib (IC50 = 4.91 µM). Furthermore, the selectivity index of compound 10n was higher than rucaparib and pemetrexed for lung cancer cells. Flow cytometry analysis showed that compound 10n significantly arrested the cell cycle in the S phase, then induced apoptosis of A549 cells (apoptosis rate is 46%), which effectively inhibited cell proliferation. Simultaneously, western blot analysis revealed that compound 10n could prevent the biosynthesis of PAR. Further analysis results revealed that compound 10n could inhibit the expression of cyclin A, down-regulate the expression of bcl-2/bax, activate caspase-3, and ultimately induce apoptosis of A549 cells. All the results indicated that compound 10n had potential research value as a novel PARP-1 inhibitor in antitumor, and it provided a new reference for further development of PARP-1 inhibitors.

19.
Food Funct ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670366

RESUMO

To investigate the mechanism of the combined effects of chlorogenic acid (CGA) and caffeine on lipid metabolism in high-fat diet-induced obese mice, eighty female ICR mice were randomly divided into eight groups and fed with a high-fat diet with/without CGA and/or caffeine for 14 weeks. The combination of CGA and caffeine effectively decreased body weight gain, intraperitoneal adipose tissue weight, serum LDL-c, FFA, TC, TG, leptin, IL-6 concentrations, and hepatic TG and TC levels and increased the serum adiponectin level. The CGA and caffeine combination also promoted the phosphorylation of AMPKα, inhibited the expressions of transcriptional regulators (SREBP-1c and LXRα), and decreased the expressions of FAS and HMGR. Besides, the expressions of ACO, ATGL and HSL were increased by the CGA and caffeine combinations. The results indicated that the combination of CGA and caffeine had anti-obesity effects and regulated lipid metabolism in high-fat diet-induced obese mice via the AMPKα-LXRα/SREBP-1c signaling pathway. Thus, chronic CGA and caffeine intakes may be potent for preventing obesity.

20.
Artif Cells Nanomed Biotechnol ; 47(1): 4120-4130, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31701767

RESUMO

Background: Gastric cancer (GC) is a global leading source of cancer-associated deaths. Circular RNAs (circRNAs) are a new type of non-coding RNA and promising biomarkers for diagnosis of multiple diseases such as cancer.Methods: Circ-PRMT5 expression was validated in 90 GC patient tissues and 6 different GC cells by qRT-PCR. Sublocalization of circ-PRMT5 in GC cells was determined in isolated nuclear and cytoplasmic RNAs. CircInteractome and miRanda were used to predict binding sites between circ-PRMT5 with micRNAs, and micRNAs with target mRNA. The correlation between genes was determined by the Pearson correlation analysis. The molecular mechanism was demonstrated by RNA in vivo precipitation, point mutation, luciferase activity and rescue experiments.Results: Circ-PRMT5 expression was significantly higher in GC than in adjacent normal tissues, and GC patients with circ-PRMT5 high expression had shorter survival times. Functionally, circ-PRMT5 silence inhibited GC cell growth and invasion. Mechanism analysis showed that circ-PRMT5 sponged miR-145/miR-1304 to upregulate MYC expression and GC development.Conclusion: Our findings demonstrated that circ-PRMT5 function as an oncogene in GC patients by targeting miR-145/miR-1304/MYC axis. High circ-PRMT5 expression may provide a poor prognostic indicator of survival in GC patients and targeting circ-PRMT5/miR-145/miR-1304/MYC axis may be a novel therapeutic strategy for GC.

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