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1.
Acad Radiol ; 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33495072

RESUMO

RATIONALE AND OBJECTIVES: To investigate the effect of intralesional heterogeneity on differentiating benign and malignant pulmonary lesions, quantitative magnetic resonance imaging (MRI) radiomics, and machine learning methods were adopted. MATERIALS AND METHODS: A total of 176 patients with multiparametric MRI were involved in this exploratory study. To investigate the effect of intralesional heterogeneity on lesion classification, a radiomics model called tumor heterogeneity model was developed and compared to the conventional radiomics model based on the entire tumor. In tumor heterogeneity model, each lesion was divided into five sublesions depending on the spatial location through clustering algorithm. From the five sublesions in multi-parametric MRI sequences, 1100 radiomics features were extracted. The recursive feature elimination method was employed to select features and support vector machine classifier was used to distinguish benign and malignant lesion. The performance of classification was evaluated with the receiver operating characteristic curve and the area under the curve (AUC) was the figure of merit. The 3-fold cross-validation (CV) with and without nesting was used to validate the model, respectively. RESULTS: The tumor heterogeneity model (AUC = 0.74 ± 0.04 and 0.90 ± 0.03, CV with and without nesting, respectively) outperforms conventional model (AUC = 0.68 ± 0.04 and 0.87 ± 0.03). The difference between the two models is statistically significant (p = 0.03) for lesions greater than 18.80 cm3. CONCLUSION: Intralesional heterogeneity influences the classification of pulmonary lesions. The tumor heterogeneity model tends to perform better than conventional radiomics model.

2.
Mikrochim Acta ; 187(12): 665, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33205310

RESUMO

A sensitive and robust fluorescent assay of 6-MP is described which relies on the facile assembly of a fluorescence nanoprobe by design of silica nanosphere encapsulated CdTe quantum dots (CdTe QDs) as scaffold, coupling with chemically tethered folic acid (FA)-protected silver nanoparticles (AgNPs) that function as responsive element. In this way a stable ternary core-shell-satellite nanostructure with dual-emission signals can be established. On binding to the target molecules, 6-MP, FA molecules initially occupied by AgNPs are liberated to give dose-dependent fluorescence emission, which can further form a self-calibration ratiometric fluorescence assay using CdTe QDs as an internal reference. The nanoprobe color vividly changes from red to blue, enabling the direct visual detection. The linear concentration range is 0.15~50 µM with the detection limit of 67 nM. By virtue of the favorable selectivity and robust assays, the nanoprobe was applied to 6-MP detection in urine samples, with recoveries from 97.3 to 106% and relative standard deviations (RSD) less than 5%. Graphical abstract.

3.
J BUON ; 25(4): 1947-1953, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33099937

RESUMO

PURPOSE: Pancreatic cancer (PC) is a lethal disease of the alimentary system and is ranked 4th in cancer-related deaths in United States. PC has a poor prognosis and limited therapeutic options. The main purpose of the current study was to demonstrate the anticancer effects of the naturally occurring Baicalein flavone in human cisplatin-resistant pancreatic carcinoma cell line CAPAN-2. METHODS: Cell viability was examined via MTT cell proliferative assay. Mitochondrial-mediated apoptosis was examined through DAPI and annexin V/propidium iodide (PI) staining using fluorescence microscopy along with estimation of apoptosis-related protein expressions like caspase-3, Bax, Bcl-2 for which western blot was used. Next, wound-healing and transwell assays were performed to find out the effects of Baicalein on cell migration and invasion, respectively. RESULTS: The results showed that Baicalein induced dose-dependent and selective anticancer effects in CAPAN-2 PC cancer cells with much less cytotoxicity to normal HTRET-HPNE cells. The antiproliferative effects of Baicalein were due to apoptosis induction as the number of apoptotic cells increased on increasing doses of the test molecule. Western blotting analysis revealed that the expressions of caspase-3 and Bcl-2 were decreased and Bax was increased. The test molecule also induced S-phase cell cycle arrest in PC cells with decreasing the cyclin-B1 expressions. Cell migration and invasion analysis revealed that Baicalein induced dose-dependent suppression in migration and invasion of CAPAN-2 PC cell line. CONCLUSION: Baicalein is a potential anticancer agent against PC cells and can be considered for PC systemic therapy provided more toxicological and in vivo studies are carried out.

4.
Biosens Bioelectron ; 170: 112662, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33032198

RESUMO

Cancer cell enumeration and phenotyping can predict the prognosis and the therapy efficacy in patients, yet it remains challenging to detect the rare tumor cells. Herein, we report an octopus-inspired, bifunctional aptamer signal amplifier-based cytosensor (OApt-cytosensor) for sensitive cell analysis. By assembling high-affinity antibodies on an electrode surface, the target cells could be specifically captured and thus been sandwiched by the cell surface marker-specific DNA aptamers. These on-cell aptamers function as electrochemical signal amplifiers by base-selective electronic doping with methylene blue. Such a sandwich configuration enables highly sensitive cell detection down to 10 cells/mL (equal to ~1-2 cells at a sampling volume of 150 µL), even in a large excess of nontarget blood cells. This approach also reveals the cell-surface markers and tracks the cellular epithelial-to-mesenchymal transition induced by signaling regulators. Furthermore, the electron-doped aptamer shows remarkable cell fluorescent labeling that guides the release of the captured cells from electrode surface via electrochemistry. These features make OApt-cytosensor a promising tool in revealing the heterogeneous cancer cells and anticancer drug screening at the single-cell level.

5.
Med Biol Eng Comput ; 58(9): 2095-2105, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32654016

RESUMO

Lung diffusion-weighted magnetic resonance imaging (DWI) has shown a promising value in lung lesion detection, diagnosis, differentiation, and staging. However, the respiratory and cardiac motion, blood flow, and lung hysteresis may contribute to the blurring, resulting in unclear lung images. The image blurring could adversely affect diagnosis performance. The purpose of this study is to reduce the DWI blurring and assess its positive effect on diagnosis. The retrospective study includes 71 patients. In this paper, a motion correction and noise removal method using low-rank decomposition is proposed, which can reduce the DWI blurring by exploit the spatiotemporal continuity sequences. The deblurring performances are evaluated by qualitative and quantitative assessment, and the performance of diagnosis of lung cancer is measured by area under curve (AUC). In the view of the qualitative assessment, the deformation of the lung mass is reduced, and the blurring of the lung tumor edge is alleviated. Noise in the apparent diffusion coefficient (ADC) map is greatly reduced. For quantitative assessment, mutual information (MI) and Pearson correlation coefficient (Pearson-Coff) are 1.30 and 0.82 before the decomposition and 1.40 and 0.85 after the decomposition. Both the difference in MI and Pearson-Coff are statistically significant (p < 0.05). For the positive effect of deblurring on diagnosis of lung cancer, the AUC was improved from 0.731 to 0.841 using three-fold cross validation. We conclude that the low-rank matrix decomposition method is promising in reducing the errors in DWI lung images caused by noise and artifacts and improving diagnostics. Further investigations are warranted to understand the full utilities of the low-rank decomposition on lung DWI images. Graphical abstract.

6.
Acta bioeth ; 26(1): 81-90, mayo 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1114601

RESUMO

Doctor moral hazard has a significant effect on the doctor-patient relationship, increases the cost of healthcare, and introduces medical risks. It is a global concern. Doctor moral hazard behaviour is evolving in response to China's healthcare reform program which was inaugurated in 2009.A scientific understanding of doctor behaviour would facilitate the prevention and control of doctor moral hazard behaviour. This study used the principles and methodology of Glaser and Strauss's grounded theory. Theoretical and snowball samplings were used to identify 60 subjects. Semi-structured in-depth interviews were conducted with each subject. Themes were identified through substantial (open) coding and theoretical coding. Six types of doctor moral hazard behaviour were extracted from the data. A behavioural model was described and diagrammed to provide a conceptual framework of current doctor moral hazard behaviour. The conceptual model of doctor moral hazard behaviour can be used in several ways to correct or prevent undesirable actions. Rules governing hospital procedures can be strengthened and enforced by supervision and punishment; the asymmetry of information between doctor and patient can be reduced; patient participation in treatment decisions can be increased; the effectiveness of medical ethics education can be improved.


Para un médico, el riesgo moral tiene un efecto significativo en la relación médico-paciente, incrementa el costo de la atención de salud e introduce riesgos en la salud. Se trata de una preocupación global. El riesgo moral del comportamiento médico ha evolucionado en respuesta al programa de reforma de atención de salud del gobierno de China, inaugurado en 2009. Un entendimiento científico del comportamiento de los médicos facilitaría la prevención y el control del riesgo moral. El presente estudio usa los principios y metodología de la teoría fundamentada de Glaser y Strauss. Se usaron muestras teóricas y multiplicativas para identificar 60 sujetos y realizar entrevistas semiestructuradas en profundidad. Los temas se identificaron mediante codificación sustancial abierta y teórica. De los datos se extrajeron seis tipos de riesgo moral del comportamiento médico. Se describió y diagramó un modelo de comportamiento para proporcionar una estructura conceptual del riesgo moral del comportamiento médico actual. El modelo conceptual de riesgo moral del comportamiento médico puede usarse de varias maneras para corregir o prevenir acciones no deseadas. Las normas procedimentales de los hospitales pueden fortalecerse y exigirse mediante supervisión y castigo; se puede reducir la asimetría de la información que se da entre el médico y el paciente, incrementar la participación del paciente en decisiones de tratamiento y mejorar la efectividad en la educación en ética médica.


Risco moral médico tem um efeito significativo na relação médico-paciente, aumenta o custo dos cuidados à saúde e introduz riscos médicos. É uma preocupação global. Comportamento de risco moral médico vem se desenvolvendo em resposta ao programa de reforma de cuidados à saúde da China, que se iniciou em 2009. Uma compreensão científica do comportamento médico facilitaria a prevenção e controle do comportamento de risco moral médico. Este estudo utilizou os princípios da metodologia da Teoria Fundamentada de Glaser e Strauss. Amostragem teóricas e por bola de neve foram utilizadas para identificar 60 participantes. Entrevistas detalhadas semi-estruturadas foram realizadas com cada participante. Temas foram identificados através de codificação (aberta) substancial e codificação teórica. Seis tipos de comportamento de risco moral médico foram obtidos dos dados. Um modelo comportamental foi descrito e diagramado de forma a fornecer um enquadre conceitual do comportamento de risco moral médico. O modelo conceitual de comportamento de risco moral médico pode ser utilizado de diversas formas para corrigir ou prevenir ações indesejáveis. Regras que governam procedimentos em hospitais podem ser fortalecidas e reforçadas por supervisão e punição; a assimetria de informações entre médicos e pacientes pode ser reduzida; a participação dos pacientes nas decisões sobre tratamento pode ser aumentada; e a efetividade da educação ética médica pode ser melhorada.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Médicos/psicologia , Reforma dos Serviços de Saúde , Moral , Relações Médico-Paciente , Médicos/ética , Padrões de Prática Médica , Comportamentos Relacionados com a Saúde , China , Comportamento de Escolha , Risco , Entrevistas como Assunto , Teoria Fundamentada , Sobremedicalização
7.
Eur Radiol ; 30(8): 4595-4605, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32222795

RESUMO

OBJECTIVES: We develop and validate a radiomics model based on multiparametric magnetic resonance imaging (MRI) in the classification of the pulmonary lesion and identify optimal machine learning methods. MATERIALS AND METHODS: This retrospective analysis included 201 patients (143 malignancies, 58 benign lesions). Radiomics features were extracted from multiparametric MRI, including T2-weighted imaging (T2WI), T1-weighted imaging (TIWI), and apparent diffusion coefficient (ADC) map. Three feature selection methods, including recursive feature elimination (RFE), t test, and least absolute shrinkage and selection operator (LASSO), and three classification methods, including linear discriminate analysis (LDA), support vector machine (SVM), and random forest (RF) were used to distinguish benign and malignant pulmonary lesions. Performance was compared by AUC, sensitivity, accuracy, precision, and specificity. Analysis of performance differences in three randomly drawn cross-validation sets verified the stability of the results. RESULTS: For most single MR sequences or combinations of multiple MR sequences, RFE feature selection method with SVM classifier had the best performance, followed by RFE with RF. The radiomics model based on multiple sequences showed a higher diagnostic accuracy than single sequence for every machine learning method. Using RFE with SVM, the joint model of T1WI, T2WI, and ADC showed the highest performance with AUC = 0.88 ± 0.02 (sensitivity 83%; accuracy 82%; precision 91%; specificity 79%) in test set. CONCLUSION: Quantitative radiomics features based on multiparametric MRI have good performance in differentiating lung malignancies and benign lesions. The machine learning method of RFE with SVM is superior to the combination of other feature selection and classifier methods. KEY POINTS: • Radiomics approach has the potential to distinguish between benign and malignant pulmonary lesions. • Radiomics model based on multiparametric MRI has better performance than single-sequence models. • The machine learning methods RFE with SVM perform best in the current cohort.

8.
Medicine (Baltimore) ; 99(10): e19185, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32150056

RESUMO

To investigate the characteristics of spiral computed tomography (CT), positron emission tomography-computed tomography (PET/CT) and clinical manifestations of talaromycosis to improve the diagnostic level and deepen its recognition in radiology.Radiological, clinical, and pathological manifestations of 15 patients of non-HIV talaromycosis confirmed by bronchofiberscope lung biopsy and/or abscess puncture fluid culture and/or blood culture and/or sputum culture were analyzed retrospectively. All patients underwent chest CT, among them, six had a brain MRI, and six had a PET/CT scan before treatment.On plain CT scan, there were multiple patches and massive consolidation in 6 patients, multiple patchy consolidations and patchy ground-glass opacities in 3 patients, solitary or multiple nodules and masses in 3 patients, multiple cavities and small nodules in 3 patients. Multiple lymphadenectasis appeared in bilateral hila, mediastinum, and neck in 10 patients. In contrast CT scan, the parenchyma of the lesions had a slight enhancement in 10 patients, moderate enhancement in 3 patients, obvious enhancement in 2 patients. Seven cases had bone destruction and hyperplasia, cranial involvement in 1 patient and liver involvement in 3 patients, respectively. On PET/CT, five patients showed elevated standard uptake value (SUV).The radiological manifestations of non-HIV talaromycosis show multiple consolidations, ground-glass opacities, multiple nodules or masses in bilateral lungs, deep-seated enlarged lymph nodes and bone destruction in multiple systems. The final diagnosis should be based on the culture of talaromycosis.


Assuntos
Pneumopatias Fúngicas/diagnóstico por imagem , Micoses/diagnóstico por imagem , Adolescente , Adulto , Idoso , Biópsia , Criança , Pré-Escolar , Diagnóstico Diferencial , Diagnóstico Precoce , Feminino , Fluordesoxiglucose F18 , Infecções por HIV , Humanos , Lactente , Pneumopatias Fúngicas/microbiologia , Pneumopatias Fúngicas/patologia , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Micoses/microbiologia , Micoses/patologia , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Compostos Radiofarmacêuticos , Estudos Retrospectivos , Tomografia Computadorizada por Raios X , Adulto Jovem
9.
J Safety Res ; 72: 29-40, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32199575

RESUMO

INTRODUCTION: Among attempts that address high incidences of fatalities and injuries in coal mines, increasing attention has been paid to management commitment to complement the traditional focus on technological advances in safety management. However, more research is needed to explain the influence of perceived management commitment, with extant research drawing commonly on Griffin and Neal (2000) to focus on safety knowledge, skills, and motivation. This study draws on social cognitive theory (Bandura, 1986) to investigate psychological capital as a link between thought process and safety behavior. METHOD: This study uses survey data from 400 frontline workers in China's coal mines to test hypotheses. RESULT: Results suggest that perceived management commitment to safety correlates positively with workers' safety compliance and participation, and four constituents of psychological capital-self-efficacy, hope, optimism, and resilience-explain the influence of perceived management commitment on safety compliance and participation. Practical Applications: Findings offer both researchers and practitioners an explanation of how perceived management commitment influences safety behaviors, and clarify the roles psychological capital constituents play in explaining the influence of perceived management commitment on safety compliance and safety participation.


Assuntos
Comportamentos Relacionados com a Saúde , Gestão da Segurança/estatística & dados numéricos , Autoeficácia , Capital Social , Adulto , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
10.
J Comput Assist Tomogr ; 44(3): 334-340, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32217894

RESUMO

OBJECTIVE: The aim of the study was to compare intravoxel incoherent motion diffusion-weighted imaging (DWI) for evaluating lung cancer using single-shot turbo spin-echo (TSE) and single-shot echo-planar imaging (EPI) in a 3T MR system. METHODS: Both single-shot TSE-DWI and single-shot EPI-DWI were scanned twice respectively for 15 patients with lung cancer. Distortion ratio, signal-to-noise ratio, and contrast-to-noise ratio were compared between the 2 techniques. The Bland-Altman analysis was performed to analyze reproducibility between the parameters of TSE-DWI and EPI-DWI. Short-term test-retest repeatability, as well as interobserver agreement, was evaluated using the coefficient of variation (CV) and the intraclass correlation coefficient (ICC). RESULT: Turbo spin-echo DWI has lower signal-to-noise ratio and similar contrast-to-noise ratio compared with EPI-DWI. Distortion ratio of TSE-DWI was significantly smaller than that of EPI-DWI. The apparent diffusion coefficient (ADC) and true diffusivity (D) of TSE-DWI showed higher values than those of EPI-DWI. The Bland-Altman analysis showed unacceptable limits of agreement between these 2 sequences. Test-retest repeatability was good for ADC and D of EPI-DWI (CV, 14.11%-16.60% and 17.08%-19.53%) and excellent for ADC and D of TSE-DWI (CV, 4.8%-6.19% and 6.05%-8.71%), but relatively poor for perfusion fraction (f) and pseudo-diffusion coefficient (D*) (CV, 25.95%-27.70% and 56.92%-71.84% for EPI, 23.67%-28.67% and 60.85%-70.17% for TSE). For interobserver agreement, both techniques were good to excellent in ADC and D (The lower limit of 95% confidence interval for ICC was almost all greater than 0.75), whereas D* and f had higher interobserver variabilities with D* of TSE-DWI showing poorest reproducibility (ICC, -0.27 to 0.12). CONCLUSIONS: Lung DWI or IVIM using TSE could provide distortion-free images and improve the test-retest robustness of ADC and D as compared with EPI-DWI; however, it might exert a negative effect on perfusion parameter D*.


Assuntos
Imagem de Difusão por Ressonância Magnética/métodos , Imagem Ecoplanar/métodos , Interpretação de Imagem Assistida por Computador/métodos , Neoplasias Pulmonares/diagnóstico por imagem , Adulto , Idoso , Feminino , Humanos , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reprodutibilidade dos Testes
11.
Cancer Manag Res ; 12: 209-219, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32021437

RESUMO

Objective: The evaluation of lymphocyte subsets is widely regarded as an important factor for monitoring tumor progression and response to therapy. This study was designed to establish a comprehensive and detailed assessment of peripheral lymphocyte subsets with a multi-parametric flow cytometry assay for response prediction and prognosis evaluation of cancer patients. Methods: Peripheral blood samples collected from 40 cancer patients and 23 age- and sex-matched healthy volunteers were tested for 29 lymphocyte subsets by flow cytometry. The univariate analysis was applied to establish the reference interval of healthy samples, and the ratio and proportion of 29 lymphocyte subsets between patient samples and healthy controls were compared to evaluate their clinical significance by Mann-Whitney U-test model. Results: The reference ranges of 29 lymphocyte subsets were established with a normal distribution and no significant differences were observed between genders. Compared with healthy control group, lower proportion and ratio of specific parameters, such as Naïve Th cells (p<0.01), Naïve Tc cells (p<0.01), CM (central memory) Tc cells (p<0.01), Naïve T cells/Memory T cells (p<0.001), Naïve T cells/EM (effector memory) T cells (p<0.001) and Naive Th cells/Memory Th cells (p< 0.001), and higher proportion and ratio of EM Th cells (p<0.001), EM Tc cells (p<0.01), effector Tc cells (p<0.05), EM Th cells/CM Th cells (p<0.01) and EM Tc cells/CM Tc cells (p<0.01), as well as Breg (p<0.001), B cells (p<0.05) and CD16-NK cells (p<0.001) were found in cancer cohorts. Conclusion: This study suggests that the changes in certain lymphocyte subsets might be helpful to evaluate the immunity of cancer patients, and holds great potential for clinical application.

12.
J Colloid Interface Sci ; 566: 473-484, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32035352

RESUMO

A facile and green method for preparation of gold/palladium (Au/Pd) bimetallic nanoparticles interleaved reduced graphene oxide (rGO) composite was presented. One-step electroreduction of Au/Pd precursors and graphene oxide synergistically produced a multilayered and well-structured nanohybrid on glassy carbon electrode, which was explored as a highly efficient electrocatalyst. This operation is easy and controllable, as compared with time-consuming and procedure-tedious hydrothermal synthesis. The morphology and chemical constituents were meticulously characterized. The remarkable electrocatalytic performance of the prepared nanohybrid was demonstrated by detection of a high-risk carcinogen pollutant, hydrazine. By optimizing the preparation condition and investigating the electrochemical behavior, we achieved the sensitive analysis of hydrazine with ultralow oxidation overpotential. Amperometry was employed for constructing the quantitative calibration curve; the steady-state current originating from hydrazine oxidation was proportional to the analytical concentration ranging from 0.1 µM to 200 µM, with the detection limit of 16 nM. Moreover, the nanohybrid displayed considerable anti-interfering ability with respect to hydrazine detection, as a variety of potentially coexisting substances produced negligible electrochemical response in the given analytical condition. Advantages including easy-to-preparation, high sensitivity and favorable selectivity, as well as broad linear response make the present method feasible for monitoring of hydrazine in water environment.

13.
Int J Occup Med Environ Health ; 33(3): 263-272, 2020 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-32108174

RESUMO

OBJECTIVES: The purpose of this research is to explore the appropriate behaviors and characteristics of Patients for Patient Safety (PFPS) against the Chinese cultural background, especially the types and internal logical relationships of behaviors of PFPS. MATERIAL AND METHODS: In this research, Glazer's methodology principle of the traditional grounded theory was adopted, and the methods of objective sampling, theoretical sampling and snowball sampling were employed. Considering the diversity of the interviewed subjects' gender, age, professional title, qualification and demographic characteristics, representatives of hospital management staff, doctors, nurses, patients and their family members from different provinces and cities across the country were selected for semi-structured in-depth interviews to assess the behaviors of PFPS against the Chinese cultural background. In addition, some PFPS reports were collected from network media to supplement the interview data. All interviews were recorded and collated into Word text documents. Qualitative research data analysis software Nvivo 12 was used to sort out the collected data, and the theme was separated out through the strategy of substantive coding and theoretical coding. RESULTS: In this research, the appropriate behaviors of PFPS were taken as the research content; 6 categories of appropriate behaviors of PFPS were separated out; and a model diagram of PFPS was constructed accordingly. CONCLUSIONS: A model diagram of the appropriate behaviors of PFPS against the Chinese cultural background was constructed to provide theoretical guidance for relevant research and practice. Int J Occup Med Environ Health. 2020;33(3):263-72.

14.
Sci Rep ; 10(1): 1425, 2020 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996724

RESUMO

Mortality of primary hypertension is high worldwide. Whether untraditional factors exist in modern life and affect the mortality is not well studied. The aim of the study was to evaluate the risk factors for fatality rate of hypertensive men in downtown area. A cross-sectional study was performed on hypertensive men, who were hospitalized into our hospital and lived in eligible urban areas. The characteristics of the patients and factors for the fatality were analyzed and of the risks or the contributors on the status were investigated. 14354 patients were identified. Mean age was 68.9 ± 12.4 year old (y) and dead ones was 75.9 ± 9.5 y. The overall hospitalized fatality was 5.9%, which was increased with age: fatality with 0.7%, 2.2%, 2.9%, 7.1%, 11.1% and 16.6% was for age group ≦ 49 y, 50-59 y, 60-69 y, 70-79 y, 80-89 y and ≧ 90 y respectively. The increased fatality was significantly positively correlated with the incidence of pneumonia, P < 0.05, r = 0.99. Pneumonia was prone to involve in men with older age and severer organ damage by hypertension. Similar to traditional risks such as coronary heart disease and stroke, pneumonia and lung cancer were also significantly associated with the fatality. Odds ratio (95% CI) for pneumonia and lung cancer were 6.18 (4.35-8.78) and 1.55 (1.14-2.11). The study provides evidence that pneumonia and lung cancer are highly associated with fatality of hypertensive men in downtown area, indicating that in order to reduce the fatality of hypertension, these lung diseases should be prevented and treated intensively in modern life.


Assuntos
Hipertensão/epidemiologia , Neoplasias Pulmonares/epidemiologia , Pneumonia/epidemiologia , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Hospitalização , Humanos , Hipertensão/mortalidade , Incidência , Neoplasias Pulmonares/mortalidade , Masculino , Pessoa de Meia-Idade , Pneumonia/mortalidade , Fatores de Risco , Análise de Sobrevida , População Urbana
15.
Cancer Lett ; 469: 419-428, 2020 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-31734356

RESUMO

The role of lncRNAs in the regulation of glutamate metabolism and metabolic reprogramming of pancreatic cancer (PC) during nutrient deprivation is largely unknown. Our study found that alpha-ketoglutarate (aKG) levels were significantly reduced in the absence of XLOC_006390. We subsequently confirmed that the decrease in aKG was mainly due to the downregulation of glutamate dehydrogenase 1 (GDH1) at the mRNA level. Therefore, we first screened transcription factors targeting the GDH1 gene promoter and confirmed that c-Myc regulates GDH1 transcription. c-Myc binds to the promoter of GDH1 and activates its transcription. Downregulation of GDH1 mRNA levels by XLOC_006390 deletion could be rescued by overexpression of c-Myc. Overexpression of XLOC_006390 promoted the protein stability of c-Myc by blocking its ubiquitination. Clinically, XLOC_006390 was positively correlated with the mRNA level of GDH1, and c-Myc positively regulated GDH1 gene expression, which was tightly associated with PC patient prognosis. The dysregulated lncRNA/c-Myc axis increased glutamate metabolism, promoting PC progression to a higher stage. Therefore, XLOC_006390/c-Myc may be a potential target for PC, and its abnormal activation also indicates the progression of PC.


Assuntos
Glutamato Desidrogenase/genética , Neoplasias Pancreáticas/genética , Proteínas Proto-Oncogênicas c-myc/genética , RNA Longo não Codificante/genética , Animais , Carcinogênese/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Ácido Glutâmico/genética , Ácido Glutâmico/metabolismo , Xenoenxertos , Humanos , Ácidos Cetoglutáricos/metabolismo , Masculino , Camundongos , Pessoa de Meia-Idade , Neoplasias Pancreáticas/patologia , Regiões Promotoras Genéticas/genética , RNA Mensageiro/genética
16.
Anal Bioanal Chem ; 412(3): 669-680, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31834450

RESUMO

A layer-by-layer self-assembled nitrogen-doped graphene/PDDA/gold nanoparticle (NDG/PDDA/GNP) composite was described. Citrate-stabilizing gold nanoparticle colloids (GNPs) were electrostatically adsorbed onto NDG nanosheets using a cationic polyelectrolyte, polydiallyldimethylammonium (PDDA), as the linker, thereby creating a high-performance electrochemical interface. The morphology and chemical composition were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy, and Raman spectroscopy. Analytical application was manifested by electrochemical sensing of uric acid (UA), a biomarker involved with a variety of clinical diseases. The prepared nanocomposite exhibited noticeable electroactivity to uric acid oxidation and can give effective peak separation with ascorbic acid and dopamine. Additionally, the nanocomposite practically averted from other potentially interferents including glucose, urea, and serotonin, thus allowing selective voltammetric detection of UA in the biological matrix. Under the optimal condition, peak currents measured by differential pulse voltammetry were proportional to UA concentrations in the range of 0.5~100 µM (R2 = 0.998), with the detection limit of 53 nM. The NDG/PDDA/GNP nanocomposite as presented herein holds potential for aiding the diagnosis of UA-associated diseases and should be a new opportunity for biochemical analysis and biosensing applications. Graphical abstract.


Assuntos
Técnicas Eletroquímicas/métodos , Ouro/química , Grafite/química , Nanopartículas Metálicas/química , Nitrogênio/química , Polietilenos/química , Compostos de Amônio Quaternário/química , Eletricidade Estática , Ácido Úrico/análise , Limite de Detecção
17.
Comput Biol Med ; 115: 103515, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31698233

RESUMO

Non-rigid image registration is prone to non-realistic deformations. In this paper, we proposed a novel landmark-correspondence detection algorithm, with which, the non-realistic deformations in image registration can be reduced. Our method consists of the following steps. First, the landmarks in the reference image are extracted by a corner detector. Then the landmarks are transferred to the template image by the proposed Multiscale Local Rigid Matching (MsLRM) algorithm. A two-stage method is designed for outlier removal before the landmark correspondences are incorporated into a FFD-based registration through a penalty term considering that the interpolating splines in FFD are highly sensitive to outliers. The proposed method was validated on both simulated images and real-world clinical lung dynamic contrast-enhanced magnetic resonance images. The results showed that the proposed MsLRM achieved sub-pixel accuracy, and was robust to local contrast changes. On clinical datasets, the MsLRM-based landmark-constrained registration improved the registration accuracy by at least 25%, compared with the state-of-the-art registration methods. It achieved an average expert landmark distance of 0.23 mm, close to the inter-observer variability of 0.17 mm. We conclude that our novel landmark-constrained registration improves registration performance on dynamic medical images and outperforms the state-of-the-art registration methods.


Assuntos
Algoritmos , Interpretação de Imagem Assistida por Computador , Pulmão/diagnóstico por imagem , Imagem por Ressonância Magnética , Reconhecimento Automatizado de Padrão , Humanos
18.
Int J Biol Sci ; 15(12): 2548-2560, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31754328

RESUMO

Cancer immunotherapy by chimeric antigen receptor-modified T (CAR-T) cells has shown exhilarative clinical efficacy for hematological malignancies. Recently two CAR-T cell based therapeutics, Kymriah (Tisagenlecleucel) and Yescarta (Axicabtagene ciloleucel) approved by US FDA (US Food and Drug Administration) are now used for treatment of B cell acute lymphoblastic leukemia (B-ALL) and diffuse large B-cell lymphoma (DLBCL) respectively in the US. Despite the progresses made in treating hematological malignancies, challenges still remain for use of CAR-T cell therapy to treat solid tumors. In this landscape, most studies have primarily focused on improving CAR-T cells and overcoming the unfavorable effects of tumor microenvironment on solid tumors. To further understand the current status and trend for developing CAR-T cell based therapies for various solid tumors, this review emphasizes on CAR-T techniques, current obstacles, and strategies for application, as well as necessary companion diagnostics for treatment of solid tumors with CAR-T cells.


Assuntos
Imunoterapia Adotiva/tendências , Neoplasias/terapia , Síndrome da Liberação de Citocina , Citocinas/metabolismo , Humanos , Imunoterapia Adotiva/efeitos adversos , Imunoterapia Adotiva/métodos , Resultado do Tratamento , Microambiente Tumoral/imunologia
19.
J Colloid Interface Sci ; 557: 825-836, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31580978

RESUMO

Measuring glucose in a convenient and economical manner is crucial for diabetes diagnostics and surveillance. Ongoing efforts are devoted to nonenzymatic sensors using functional nanomaterials. Drawbacks due to costly and cumbersome process, however, hamper the practicality. Here, we report the facile preparation of Cu/Ni bimetallic nanocatalyst toward glucose electrooxidation. Carboxylated multi-walled carbon nanotubes were chemically grafted onto indium tin oxide glass via silanization reaction and amide coupling reaction, providing distinct nucleation sites for Cu/Ni bimetallic electrocatalyst prepared by in-situ succinct electrodeposition, which synthetically created a three-dimensional electron transfer network. The surface morphology and chemical constituents were characterized by scanning electron microscopy, transmission electron microscopy, X-ray energy dispersive spectroscopy, X-ray photoelectron spectroscopy, infrared spectroscopy and atomic force microscopy. The prepared electrocatalyst displayed ultrahigh electrochemical activity; the catalytic current density for glucose oxidation was found to be over 6.7 mA mM-1 cm-2. The linear response spanned three orders of magnitude of glucose concentration ranging from 1 µM to 1 mM. Analytical parameters such as accuracy, reproducibility, specificity and stability have also been validated. Importantly, we reveal that Ni plays a dominant role over Cu in electrocatalytic oxidation of glucose, thus bettering our understanding and strategy for nonenzymatic glucose sensor design. Advantages of the glucose sensor presented include easy bulk preparation, low cost, and ready-to-use.


Assuntos
Ligas/química , Cobre/química , Glucose/análise , Nanopartículas Metálicas/química , Nanotubos de Carbono/química , Níquel/química , Técnicas Biossensoriais , Catálise , Técnicas Eletroquímicas , Eletrodos , Limite de Detecção , Oxirredução , Reprodutibilidade dos Testes , Propriedades de Superfície
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