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1.
J Clin Nurs ; 28(23-24): 4488-4495, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31410906

RESUMO

AIMS AND OBJECTIVES: To explore whether the risk of peripheral venous catheters failure remained constant throughout catheter use in adult patients. BACKGROUND: Peripheral venous catheters, widely used in adult patients, may have a critical threshold dwell time associated with increased risk of catheter failure. DESIGN: Prospective, observational study. We have complied with the STROBE checklist of items. METHODS: This study was conducted from July-October 2018 in Hunan, China. Data on patient factors, catheter factors and catheter failure events were collected. Poisson regression was used to assess the effect of catheter dwell time on catheter failure while adjusting for other variables. RESULTS: A total of 1,477 patients were included in the analysis. There were 854 cases (57.8%) of catheter failure. The median dwell time to catheter failure was 52 hr (interquartile range: 36-73 hr). The incidence rate of catheter failure significantly increased by 1.1%/h in the first 38 hr after catheter insertion. From 39-149 hr, the incidence rate significantly decreased, and at >149 hr, there was no significant change in the incidence rate. Meanwhile, factors such as vascular quality and infused drugs showed having an impact on catheter failure events. CONCLUSIONS: The risk of catheter failure may not remain constant throughout the dwell time. The results suggest that nurses should assess the insertion site frequently in the first 38 hr. RELEVANCE TO CLINICAL PRACTICE: The significant increase in the risk of catheter failure per hour may warrant close and frequent inspection of insertion site during the first 38 hr.


Assuntos
Cateterismo Periférico/efeitos adversos , Cateteres de Demora/efeitos adversos , Falha de Equipamento/estatística & dados numéricos , Adulto , Cateterismo Periférico/enfermagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Medição de Risco , Fatores de Tempo
2.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(3): 791-4, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-25208414

RESUMO

To prepare myclobutanil molecularly imprinted polymer, a method was established for the choice of the appropriate functional monomer and its dosage. UV spectra was applied to study the combination form, the effect intensity, the optimal concentration ratio and the numbers of binding sites between myclobutanil and methyl acrylic acid (MAA) or acrylamide (AM) functional monomer. The results showed that hydrogen-bonding interaction could be formed between myclobutanil and methyl acrylic acid (MAA) or acrylamide (AM) functional monomer. The pi electron of the triazole ring conjugated double bond in my clobutanil could transit to pi* conjugate antibonding orbital when it absorbed energy. The formation of hydrogen bond could make pi-->pi* absorption band transit. Maximum absorption wavelength produced red shift with the increase in the functional monomer concentration in the system. The research revealed that the optimal concentration ratios between myclobutanil and the two monomers were c(M):c(MAA) = 1:4, c(M):c(AM) = 1:2. Myclobutanil and the both the functional monomers had the bonding ability, and strong bonding force. The prepared molecularly imprinted polymer using AM as a functional monomer had better stability and specificity of recognition for myclobutanil.

3.
Yao Xue Xue Bao ; 46(4): 438-42, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21751497

RESUMO

The inhibitory ratio (1%) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and 1% was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC50) was about 2.26 mg x mL(-1). There was a good relationship between the logarithm concentrations of artificial musk and 1% when the concentrations of artificial musk ranged from 0.31-20.0 mg x mL(-1). The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.


Assuntos
Anti-Inflamatórios/farmacologia , Inibidores de Ciclo-Oxigenase 2/farmacologia , Ciclo-Oxigenase 2/metabolismo , Ácidos Graxos Monoinsaturados/farmacologia , Técnicas Imunoenzimáticas/métodos , Concentração Inibidora 50 , Relação Quantitativa Estrutura-Atividade
4.
Yao Xue Xue Bao ; 46(2): 193-7, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21542291

RESUMO

This paper is to report the investigation on the metabolic behavior of Staphylococcus aureus (S. aureus) after given Qingkailing injection, and with the aim of seeking for a new quality control method based on biological assessment. The growth thermogenic curves of S. aureus were determined by microcalorimetry and analyzed by computer. The results showed that in the concentration range of (0-5.00%), the growth thermogenic curves of S. aureus were declined and removed back with increasing dosage of Qingkailing injection; the main parameters (T1, T2, k1, P1, P2 and I) and the dosage of Qingkailing injection have good correlation. The 50% inhibiting dosage is 3.26 %, and the optimal inhibiting dosage is 5.47%. Difference could be detected among the Qingkailing injection samples from different factories and different batches. It is proved that Qingkailing injection could inhibit the metabolic behavior of S. aureus, and microcalorimetry might be applied in the quality assessment of Qingkailing injection.


Assuntos
Calorimetria/métodos , Medicamentos de Ervas Chinesas/farmacologia , Plantas Medicinais/química , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/metabolismo , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/isolamento & purificação , Controle de Qualidade , Staphylococcus aureus/crescimento & desenvolvimento , Termodinâmica
5.
J Hazard Mater ; 179(1-3): 742-7, 2010 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-20400224

RESUMO

The effects of two bile acid derivatives, cholic acid (CA) and deoxycholic acid (DCA) on Staphylococcus aureus (S. aureus) growth were investigated and compared by microcalorimetry coupled with multiple analytical methods. The heat power (HP)-time curves of S. aureus growth affected by CA and DCA were studied by similarity analysis (SA), respectively. Then the quantitative thermo-kinetic parameters obtained from these curves were investigated by the multivariate analysis of variance (MANOVA) and principal component analysis (PCA). By analyzing the two main parameters, growth rate constant k(2) of the second exponential phase and the heat power P(2) of the second highest peak, together with the minimum inhibitory concentration (MIC) values of 10 microg/mL for CA and 20 microg/mL for DCA, it could be concluded that the antibacterial effect of CA was stronger than that of DCA. The existence of alpha-OH at C-7 position of steroid nucleus of bile acid derivatives enhanced the hydrophilicity of compound CA and its inhibitory effect on S. aureus. This study provides a useful method and idea to accurately evaluate the antibacterial effects of bile acid derivatives, which provides some references for screening out new antibacterial agents with high efficacy and low toxicity.


Assuntos
Ácido Cólico/farmacologia , Ácido Desoxicólico/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Análise de Variância , Calorimetria , Meios de Cultura , Cinética , Testes de Sensibilidade Microbiana , Análise de Componente Principal , Solventes , Termodinâmica
6.
Zhong Yao Cai ; 33(12): 1904-8, 2010 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21548370

RESUMO

OBJECTIVE: To investigate the effects of four original constituents (Loganin, Morroniside, Peoniflorin, Paeonol) of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte, and optimize the best concentration by the orthogonal test. METHODS: The rat preadipocytes were cultured, then above four original constituents according to 4 factors and 3 levels to make L9 (3(4)) orthogonal test, and the control was performed at the same time. The proliferation of preadipocytes was determined by MTT method, and the accumulation of cellular lipid was determined by Oil Red O staining, the differences between factors and variances were compared by the value of absorbance. RESULTS: The combination of four original constituents of Liu-wei Di-huang pills could stimulate rat preadipocyte proliferation and inhibited its lipid accumulation. Both the effects were highly significant (P < 0.01) with the combination of A3 B1 C3 D2; Compared with the monosomic group, the effects of the A3 B1 C3 D2 was highly significant (P < 0.01). CONCLUSION: Compared with the monosomic group, the effects of the combination of the four original constituents of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte were highly significant. This combination consists of Loganin, morroniside with high doses, Paeonol, Peoniflorin with mid-dose and low-dose, respectively. Regnesent the overall regulatory role of synergy and officiencg.


Assuntos
Adipócitos/citologia , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Acetofenonas/administração & dosagem , Acetofenonas/farmacologia , Adipócitos/efeitos dos fármacos , Animais , Benzoatos/administração & dosagem , Benzoatos/farmacologia , Hidrocarbonetos Aromáticos com Pontes/administração & dosagem , Hidrocarbonetos Aromáticos com Pontes/farmacologia , Células Cultivadas , Combinação de Medicamentos , Sinergismo Farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Gorduras/metabolismo , Glucosídeos/administração & dosagem , Glucosídeos/farmacologia , Glicosídeos/administração & dosagem , Glicosídeos/farmacologia , Iridoides/administração & dosagem , Iridoides/farmacologia , Masculino , Monoterpenos , Plantas Medicinais/química , Ratos , Ratos Wistar
7.
Fen Zi Xi Bao Sheng Wu Xue Bao ; 42(2): 89-94, 2009 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-19537191

RESUMO

Pollens of Trititrigia substitution line SN0095 were radiated with 60Co-gamma ray and cytological characters of the progenies M1 and M2 were studied. The results showed that variations with different frequencies and chromosome numbers (2n=41, 2n=40 and 2n=39) were observed in both M1 and M2. Abnormal phenomena, such as univalents, multivalents, chromosome fragments, laggard chromosomes, chromosome bridges and micronucleus, appeared in high frequencies during the meiosis. It suggested that irradiation could promote changes of chromosome number and configuration efficiently, and could lead to rearrangements or translocations between chromosomes. The M2 progenies were examined using BARC159(240), a Thinopyrum intermedium-specific SSR marker of SN0095. Most M2 plants carried the locus BARC159(240), which indicated that the specific segment involving the locus BARC159(240) of Thinopyrum intermedium existed in these plants. However, a band of common wheat YN15 disappeared in a few plants although the locus BARC159(240) existed, which implied that chromosome rearrangements may have occurred in these plants.


Assuntos
Cromossomos de Plantas/efeitos da radiação , Genoma de Planta/efeitos dos fármacos , Meiose/efeitos dos fármacos , Pólen/efeitos da radiação , Translocação Genética/efeitos dos fármacos , Triticum/efeitos dos fármacos , Pareamento Cromossômico/efeitos dos fármacos , Glutens , Pólen/genética , Radiação , Triticum/genética
9.
Fen Zi Xi Bao Sheng Wu Xue Bao ; 41(2): 139-44, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18567504

RESUMO

A germplasm line Shannong05078 was developed from the progenies of Am3 x Triticum aestivum-Dasypyrum villosum amphidiploid, the purpose of this study is to ascertain the genome composition of Shannong05078 and then supply wheat breeding with reference. The results showed that Shannong05078 was stable in morphology characters and presenting favorable agronomic traits, it was resistant to powdery mildew and rust. Its protein content was higher, and the HWM-GS of it was 7+8, 5+10. The chromosome number of Shannong05078 was 2n=28, and the chromosome configuration was 2n=14 II at the PMC MI. SSR analysis showed that Shannong05078 contained the whole A and B genomes, and had little genetic materials from D genome. A specific primer of Dasypyrum villosum (phv29) was used to identify the genetic materials form Dasypyrum villosum in Shannong05078, and specific band of Dasypyrum villosum appeared in it. It can be deduced that the genomic composition of Shannong05078 is 2n=AABB=28, and genetic materials of D genome and Dasypyrum villosum were translated into Shannong05078.


Assuntos
Quimera/genética , Cruzamentos Genéticos , Poaceae/genética , Triticum/genética , Quimera/metabolismo , Cromossomos de Plantas/genética , Genoma de Planta , Hibridização Genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Poaceae/metabolismo , Triticum/metabolismo
10.
Zhonghua Liu Xing Bing Xue Za Zhi ; 28(1): 74-7, 2007 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-17575938

RESUMO

OBJECTIVE: To determine the distribution and virologic characteristics of HBV genotypes, sub-type and possible association with the severity of liver disease. METHODS: 884 patients infected with HBV were enrolled from 8 provinces in China. HBV genotype and sub-type was determined, using PCR-RFLP method. RESULTS: The most common HBV genotypes were B (20.77% ) and C (78.22 % ) but only 1 patient showed genotypes D. We found sub-type Ba in patients with genotype B, C1 and C2 sub-type in patients with genotype C. Genotype C (83.62%) and sub-type C2 (90.32%) were predominant in northern China. Patients with genotype B were much younger than those with genotype C. There was no significant difference between patients with sub-type C1 and C2. There was no significant difference in liver function and serum HBV-DNA load between patients with genotype B and C,or between patients with sub type C1 and C2. However, hepatic inflammation and fibrosis score in patients with genotype B were significantly lower than those with genotype C. CONCLUSION: There were no significantly differences in liver function and HBV-DNA load between patients with genotype B and C, or between patients with sub-type C1 and C2. Hepatic inflammation and fibrosis score in patients with genotype B were significantly lower than those with genotype C. Genotype C/sub-type C2 were preponderance in northern China.


Assuntos
Vírus da Hepatite B/genética , Vírus da Hepatite B/isolamento & purificação , Hepatite B/virologia , Adulto , Idoso , China , Feminino , Genótipo , Hepatite B/fisiopatologia , Vírus da Hepatite B/classificação , Vírus da Hepatite B/imunologia , Vírus da Hepatite B/fisiologia , Humanos , Fígado/imunologia , Fígado/fisiopatologia , Fígado/virologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Carga Viral , Adulto Jovem
11.
Shi Yan Sheng Wu Xue Bao ; 38(2): 126-32, 2005 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16011245

RESUMO

Factors influencing genomic in situ hybridization (GISH) used in the body cells of Triticeae crops including Octoploid Tritileymus, Octoploid Trititrigia, Octoploid Tritelytrigia and wheat-Elytrigia intermedium alien disomic addition line were analyzed. The results indicated that ideal GISH effects were based on the high quality chromosome preparations containing more cell mitotic metaphase, better dispersed chromosomes and no foreign matter. The critical factors were suitable ratio of probe DNA to blocker DNA, and the control of elution condition. In addition, some abnormal phenomena, such as chromosome loss, no hybridization signal in alien chromosome, strong or weak hybridization signal, poor or overabundant hybridization signal (high hybridization background), noisy hybridization signal and hybridization spots, were preliminarily analyzed. Proposals and solutions for these problems were further brought forth.


Assuntos
Hibridização de Ácido Nucleico/genética , Poaceae/genética , Cromossomos de Plantas/genética , Poliploidia
12.
Shi Yan Sheng Wu Xue Bao ; 38(2): 133-40, 2005 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16011246

RESUMO

6 alien disomic addition lines (line0605; line0607, line0609, line0610, line0611, line0625), which were selected from 59 wheat-Elytrigia intermedium hybridization germ plasm lines, were identified by using the methods of morphology, powdery mildew resistance identifications, cytology and RAPD analysis. Morphogy results showed that they all had the better agronomic characters of their parents; Cytogic results showed that the chromosome configuration at PMC M I was 2n = 22 II; 209 random primers were used for RAPD amplification, result showed 5 of them amplified the specific bands of Elytrigia intermedium parents of 6 disomic addition lines, which could be used as RAPD markers for alien addition chromosomes. Results of powdery mildew resistance showed that line0605 was immune, line0607 was intermediately resistant, line 0610 and line0625 were highly resistant, and line0609 and line0611 were intermediately sensitive.


Assuntos
Poaceae/genética , Triticum/genética , Cromossomos de Plantas/genética , Hibridização Genética , Poaceae/fisiologia , Técnica de Amplificação ao Acaso de DNA Polimórfico , Triticum/fisiologia
13.
Yi Chuan ; 26(4): 481-5, 2004 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-15640045

RESUMO

In the progenies of octoploid Triticale Jinsong49 crossed with octoploid Tritileymus, a stable germplasm line named Shannong 030-1 was obtained. Its chromosome number was 42, and 21 bivalents could be observed at PMC MI. FISH analysis by using genomic DNAs from S. cereale and genomic DNAs from L. mollis as probes, respectively, showed that Shannong 030-1 contained a pair of translocation chromosome between rye and wheat, and had no genetic materials from L. mollis. C-banding analysis showed that it was translocation lines of 1RS.1BL. Resistance identification showed Shannong 030-1 was immune to powdery mildew.


Assuntos
Cromossomos de Plantas/genética , Cruzamentos Genéticos , Grão Comestível/genética , Translocação Genética , Triticum/genética , Bandeamento Cromossômico , Grão Comestível/microbiologia , Fungos/isolamento & purificação , Fungos/patogenicidade , Hibridização in Situ Fluorescente , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Triticum/microbiologia
14.
Magn Reson Imaging ; 21(5): 503-10, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12878260

RESUMO

Absolute quantification of cerebral blood flow (CBF), cerebral blood volume (CBV), and mean transit time (MTT) are of great relevance for clinical applications. One of the widely used methods for quantification of these parameters is gamma-variate fitting. Traditional nonlinear regression methods for gamma-variate fitting are inaccurate and computationally demanding. In this study, we developed an adaptive total least square method (ATSSL) to fit a gamma-variate function to the delayed concentration-time course. For each concentration-time curve, the beginning and ending time point of the curve are adaptively determined online. After the curves were fitted, a robust method for automatically determination of arterial input function (AIF) from whole and region of interest (ROI) was developed. Using the obtained AIF and fitted gamma-variate concentration-time curve, the MTT, CBV, and CBF were calculated by utilizing singular value decomposition algorithm. Computer simulations show that the suggested method is adaptive, reliable, and insensitive to noise. Comparison with the traditional nonlinear regression method indicated that the presented method is more accurate and faster to determine the CBV, CBF and MTT.


Assuntos
Encéfalo/anatomia & histologia , Circulação Cerebrovascular , Imagem por Ressonância Magnética , Simulação por Computador , Humanos , Análise dos Mínimos Quadrados
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