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1.
Mol Cancer Ther ; 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35545004

RESUMO

The lives of ovarian cancer patients are threatened largely due to metastasis and drug resistance. Endogenous peptides attract increasing attention in oncologic therapeutic area, a few anti-tumor peptides have been approved by the food and drug administration (FDA) for clinical use over the past decades. However, only few peptides or peptide-derived drugs with anti-ovarian cancer effects have been identified. Here we focused on the biological roles and mechanism of a peptide named PDHPS1 in ovarian cancer development. Our results indicated that PDHPS1 reduced the proliferation ability of ovarian cancer cells in vitro and inhibited the ovarian cancer growth in vivo. Peptide pull down and following mass spectrometry, western blot and qRT-PCR revealed that PDHPS1 could bind to protein phosphatase 2 phosphatase activator (PTPA), an essential activator of protein phosphatase 2A (PP2A), which resulted in increase of phosphorylated YAP, further inactivated YAP and suppressed the expression of its downstream target genes. Flow cytometry, cell membrane permeability test and immunohistochemical staining study demonstrated that there are no observable side effects of PDHPS1 on normal ovarian epithelium and hepatorenal function. Besides, modification of membrane penetration could improve the physicochemical properties and biological activity of PDHPS1. In conclusion, our study demonstrated that the endogenous peptide PDHPS1 serves as an anti-tumor peptide to inhibit YAP signaling pathway though interacting with PTPA in ovarian cancer.

2.
Bioresour Technol ; : 127298, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35569710

RESUMO

Immobilized microalgae are good for wastewater treatment and biomass production. This study investigated treatment efficiency of a continuously operated system employing immobilized microalgae for secondary effluent of wastewater treatment plants, as well as the effectiveness on induction of valuable products, harvesting and dewatering of microalgae biomass. Under semi-continuous operation condition, microalgal dry weight increased 4.75 times within 2 d, associated with the highest removal rate of ammonia and phosphate at 28.95 mg/L·d and 4.83 mg/L·d, respectively. An immobilized microalgae membrane bioreactor (iMBR) was continuously operated for a month. The harvested immobilized microalgae beads were transferred to induction stage to obtain 4.5 times increase of lipid content per cell within 2 d. Immobilized microalgae performed 1.9 cm/s settling velocity and 97% water removal efficiency around 40 °C. A prospective integrated process on resource recovery and carbon neutrality was proposed for wastewater treatment, induction, harvesting and dewatering of immobilized microalgae cells.

3.
Mol Neurobiol ; 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35556215

RESUMO

Chronic stress is an important risk factor for mood disorders including depression. The decreased level of CREB (cAMP-responsive element binding)-regulated transcription coactivator 1 (CRTC1) expression in hippocampus may be involved in depression-like behavior in some stress-induced depression models. But the mechanism of CRTC1 in mediating depression-like behavior remains unknown. In this study, chronic unpredictable mild stress (CUMS)-treated mice showed depression-like behavior accompanied by the downregulation of CRTC1 in the hippocampus. Adeno-associated virus (AAV)-CRTC1-mediated overexpression of CRTC1 in the hippocampus by stereotactic brain injection could significantly prevent depression-like behavior in CUMS-treated mice. The above data reveal that the downregulation of hippocampal CRTC1 expression participates in CUMS-induced depression-like behavior. In order to explore the key targets regulated by CRTC1, AAV-mediated CRTC1 short hairpin (shRNA) was constructed to achieve knockdown of CRTC1 in the hippocampus, and then the hippocampi were collected for RNA-sequencing (RNA-seq). The RNA-seq data show that upregulated genes were enriched in stress and immune system-associated GO terms and pathways such as response to stress and external stimulus and regulation of immune response and that downregulated genes were enriched in neural activity such as synaptic transmission and cognitive behavior. We further provided RT-qPCR data that the inflammation-related factors including Gpr84, Tlr2, Lyz2, and Icam1 were significantly upregulated in the hippocampus of both CUMS- and CRTC1 shRNA-induced models, some of them were also validated in protein levels by Western blotting. We propose a hypothesis that CUMS induces downregulation of CRTC1, which might lead to depression-like behavior via neuroinflammation pathway. This study provides new explanation for the inflammatory hypothesis of depression and some clues for exploring the molecular mechanism of CRTC1 regulation.

4.
Small ; : e2201137, 2022 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-35527344

RESUMO

Cost-effective electrocatalysts for the hydrogen evolution reaction (HER) spanning a wide pH range are highly desirable but still challenging for hydrogen production via electrochemical water splitting. Herein, Mo5 N6 -MoS2 heterojunction nanosheets prepared on hollow carbon nanoribbons (Mo5 N6 -MoS2 /HCNRs) are designed as Mott-Schottky electrocatalysts for efficient pH-universal HER. The in-plane Mo5 N6 -MoS2 Mott-Schottky heterointerface induces electron redistribution and a built-in electric field, which effectively activates the inert MoS2 basal planes to intrinsically increase the electrocatalytic activity, improve electronic conductivity, and boost water dissociation activity. Moreover, the vertical Mo5 N6 -MoS2 nanosheets provide more activated sites for the electrochemical reaction and facilitate mass/electrolyte transport, while the tightly coupled HCNRs substrate and metallic Mo5 N6 provide fast electron transfer paths. Consequently, the Mo5 N6 -MoS2 /HCNRs electrocatalyst delivers excellent pH-universal HER performances exemplified by ultralow overpotentials of 57, 59, and 53 mV at a current density of 10 mA cm-2 in acidic, neutral, and alkaline electrolytes with Tafel slopes of 38.4, 43.5, and 37.9 mV dec-1 , respectively, which are superior to those of the reported MoS2 -based catalysts and outperform Pt in overall water splitting. This work proposes a new strategy to construct an in-plane heterointerface on the nanoscale and provides fresh insights into the HER electrocatalytic mechanism of MoS2 -based heterostructures.

5.
Neurology ; 2022 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-35473763

RESUMO

OBJECTIVE: To investigate the association of change in leisure-time physical activity (LTPA) occurring during the post-retirement period with incident stroke. METHODS: The present study enrolled 12 644 retired workers from the Dongfeng-Tongji cohort (DFTJ) during April to October 2013. The change in LTPA was categorized as follows according to whether their LTPA time meeting the World Health Organization (WHO) recommended minimum (at least 150 minutes per week): (i) stayed inactive at both surveys; (ii) stayed inactive at 2008 survey but became active at 2013 survey; (iii) stayed active at 2008 survey but became inactive at 2013 survey; (iv) stayed active at both surveys. We used multivariable-adjusted Cox proportional hazards regression models to examine the association between change in LTPA and the risk of incident stroke. RESULTS: During 68 476 person-years of follow-up, we documented 549 incident stroke cases, including 434 incident ischemic stroke (IS) cases and 115 incident hemorrhagic stroke (HS) cases. Compared with participants who stayed active at both 2008 and 2013 surveys, those stayed active at 2008 survey but became inactive at 2013 survey had significant higher risks of incident total stroke (hazard ratio [HR] = 1.30; 95% confidence interval [95% CI]: 1.03, 1.65) and HS (HR = 2.34; 95% CI: 1.51, 3.63). When stratified by body mass index (BMI) categories, a significant elevated risk of total stroke was seen among over-weight participants who stayed active at 2008 survey but became inactive at 2013 survey (HR = 1.65; 95% CI: 1.20, 2.27). The risk of incident stroke decreased with increasing LTPA levels between two surveys (HR of per 150 minutes/week increase of LTPA: HR=0.97; 95% CI: 0.94, 1.00). In addition, we found that compared with participants who maintained their BMI and stayed active at both 2008 and 2013 surveys, those both inactive or became inactive had higher risks of stroke (HR = 2.13, 95% CI: 1.09, 4.15; HR = 1.50, 95% CI: 1.07, 2.08; respectively). CONCLUSION: Among Chinese older adults, increasing LTPA levels during the post-retirement period was associated with a lower risk of incident stroke. Retired individuals should be encouraged to participate in LTPA more frequently to lessen future risk of incident stroke.

6.
J Phys Chem Lett ; 13(15): 3332-3337, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35394781

RESUMO

In photocatalytic reactions, the activation of H2O is very important for achieving high energy conversion efficiency. However, its activation mechanism under photoirradiation is still not fully understood. Here, on the basis of first-principles calculations, the role of photogenerated holes on the activation of H2O is investigated in a typical photocatalytic material C2N. The H2O molecule adsorbs at the six-membered N pore of C2N with a dual H-bonding configuration. Due to the electrostatic repulsion between the O atom of H2O and six N atoms of C2N, the energy level of the H2O molecule's highest occupied molecular orbital is raised significantly to exceed the valence band maximum of C2N, so that the photogenerated holes in C2N can be quickly captured by the H2O molecule. The captured photogenerated holes boost the activation of H2O and reduce the dissociation energy barrier from 1.61 to 0.69 eV. Besides, p-type defects of C2N have similar effects as photogenerated holes.

7.
Research (Wash D C) ; 2022: 9857631, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35360648

RESUMO

Searching ferromagnetic semiconductor materials with electrically controllable spin polarization is a long-term challenge for spintronics. Bipolar magnetic semiconductors (BMS), with valence and conduction band edges fully spin polarized in different spin directions, show great promise in this aspect because the carrier spin polarization direction can be easily tuned by voltage gate. Here, we propose a standard high-throughput computational screening scheme for searching BMS materials. The application of this scheme to the Materials Project database gives 11 intrinsic BMS materials (1 experimental and 10 theoretical) from nearly ~40000 structures. Among them, a room-temperature BMS Li2V3TeO8 (mp-771246) is discovered with a Curie temperature of 478 K. Moreover, the BMS feature can be maintained well when cutting the bulk Li2V3TeO8 into (001) nanofilms for realistic applications. This work provides a feasible solution for discovering novel intrinsic BMS materials from various crystal structure databases, paving the way for realizing electric-field controlled spintronics devices.

8.
R Soc Open Sci ; 9(4): 211901, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35425631

RESUMO

Agricultural landscape patterns can affect the population dynamics of pest insects. We selected four landscapes (flower field, mountain, river and urban) based on principal components analysis in Yunnan Province, south China. Through systematic investigation carried out in tomato fields, we intended to clarify the population dynamics and densities of Bemisia tabaci under different landscape types. During the main activity period of B. tabaci, the population densities of B. tabaci nymphs in tomato fields in the river and the urban landscape types were the highest compared to the other landscape types; the population densities of female adults in tomato fields in the river landscape type were also the highest. While the population densities of B. tabaci nymphs and female adults in the flower landscape type, no more than five individuals (ind.) 100 cm-2 leaf in both years, were the lowest. The density of B. tabaci nymphs in the middle position of tomato plants was higher than those in the other positions, while the density of adults in the upper position of tomato plants was higher, regardless of landscape types. Our findings showed that the population growth of B. tabaci can be easily controlled by the flower landscape type.

9.
Front Cardiovasc Med ; 9: 817396, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35252396

RESUMO

OBJECTIVE: The present study aimed to explore the prognostic value of trimethylamine N-oxide (TMAO) in heart failure (HF). METHODS: PubMed, Excerpta Medica Database (EMBASE), Cochrane Library, Web of Science, Wanfang Database, SINOMED, China Science and Technology Journal Database (VIP), and China National Knowledge Infrastructure (CNKI) were searched up to June 1, 2021. Studies recording the major adverse cardiovascular events (MACEs) or all-cause mortality in HF patients and their circulating TMAO concentrations were included. Meta-analysis was performed using Stata 13.0. RESULTS: Ten articles (12 studies) involving 13,425 participants from 2014 to 2021 were considered. Compared to low-level TMAO, elevated TMAO was correlated with MACEs and all-cause mortality in HF (RR: 1.28, 95% CI: 1.17, 1.39, P < 0.0001, random-effects model and RR: 1.35, 95% CI: 1.28, 1.42, P < 0.0001, random-effects model, respectively). Consistent results were obtained in all examined subgroups as well as in the sensitivity analysis. CONCLUSION: Elevated TMAO may be an adverse prognostic indicator in patients with HF. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=267208.

10.
J Psychiatr Res ; 149: 124-127, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35272209

RESUMO

Light therapy has been used as a non-pharmacologic treatment to modulate biorhythms in patients with mental and psychological conditions. These conditions include affective disorders and depression. Delirium is a syndrome characterized by an acute change in a patient's mental status. We hypothesized that light therapy might suppress delirium in patients with Alzheimer's disease (AD). A 4-week randomized controlled trial was conducted in which AD participants were randomly assigned to a treatment group or a control group. Delirium, defined by the Confusion Assessment Method (CAM), was evaluated at baseline and after 4 weeks. The Neuropsychiatric Inventory (NPI) and Zarit Caregiver Burden Interview (ZBI) were also conducted to assess the behavior of patients and the burden of their caregivers. For this study, 61 participants were initially recruited. A total of 34 and 27 participants were included in the treatment and control groups, respectively. After treatment with light therapy, the CAM score decreased during the second and fourth week. The NPI score in the therapy group also decreased during the second and fourth week. From the caregiver's perspective, after light therapy, the ZBI score significantly decreased during the second and fourth week. Compared with the control group, patients who underwent CAM and NPI assessments showed a small but significant improvement after 4 weeks of light therapy. In conclusion, a course of 4-week light therapy significantly suppressed delirium in patients with AD. The combined effects of light therapy and conventional treatment were superior to that of conventional treatment alone.


Assuntos
Doença de Alzheimer , Delírio , Idoso , Doença de Alzheimer/complicações , Doença de Alzheimer/psicologia , Doença de Alzheimer/terapia , Cuidadores/psicologia , Delírio/etiologia , Delírio/terapia , Humanos , Fototerapia
11.
Nurs Health Sci ; 24(1): 312-321, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35157362

RESUMO

Bone marrow-derived mesenchymal stem cells (BMSCs) and their exosomes are of great significance for the recovery of cardiac function in patients with myocardial infarction (MI). However, the underlying mechanisms of BMSCs applied to MI treatment remain unclear. Fluorescence-activated cell sorting (FACs) are performed to assess the apoptosis, reactive oxygen species levels and glucose uptake capacity of BMSCs. Reverse transcription polymerase chain reaction is conducted to detect the levels of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), transforming growth factor-beta 1, connective tissue growth factor, and platelet-derived growth factor. The levels of apoptosis-related proteins were detected by Western blot. The levels of VEGF, bFGF, HGF, and IGF were assessed by enzyme-linked immunosorbent assay. The biochemical kits are applied to detect the levels of malondialdehyde, superoxide dismutase, and adenosine triphosphate/adenosine diphosphate. 2,3,5-triphenyltetrazolium and Masson staining and immunofluorescence are performed to assess myocardial function of rats. Angiopoietin-like protein 4 (ANGPTL4) alleviates apoptosis and oxidative stress of BMSCs induced by serum deprivation and hypoxia; ANGPTL4 activates paracrine and accelerate metabolic energy of BMSCs; and ANGPTL4 treated-BMSCs alleviate myocardial injury of rats with MI. ANGPTL4 treated-BMSCs alleviate myocardial injury in rats with MI, indicating the combination therapy of ANGPTL4 and BMSCs may alleviate myocardial injury in rats with MI.


Assuntos
Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Infarto do Miocárdio , Animais , Medula Óssea/metabolismo , Humanos , Células-Tronco Mesenquimais/metabolismo , Infarto do Miocárdio/terapia , Ratos , Fator A de Crescimento do Endotélio Vascular/metabolismo
12.
Org Lett ; 24(6): 1388-1393, 2022 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-35138108

RESUMO

A hybrid trans-AT PKS/NRPS gene cluster htm was identified with defined boundaries for hangtaimycin biosynthesis in Streptomyces spectabilis CPCC200148. Deoxyhangtaimycin, a new derivative of hangtaimycin, was identified from the htm11 gene knockout mutant. In vitro biochemical assays demonstrated that the cytochrome P450 monooxygenase Htm11 was responsible for the stereoselective hydroxylation of deoxyhangtaimycin to hangtaimycin. More importantly, deoxyhangtaimycin showed activity against influenza A virus at the micromolar level, highlighting its potential as an antiviral lead compound.


Assuntos
Antivirais/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Glicina/metabolismo , Antivirais/química , Antivirais/farmacologia , Biocatálise , Glicina/química , Glicina/farmacologia , Hidroxilação , Vírus da Influenza A/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular
13.
Mol Med Rep ; 25(4)2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35191523

RESUMO

It is known that increased inflammation and extracellular matrix (ECM) degradation in chondrocytes can promote the development of osteoarthritis (OA). The FXYD domain containing ion transport regulator 5 (Fxyd5) has been found to promote chronic inflammatory responses. The present study aimed to investigate the role of Fxyd5 in OA. Murine ATDC5 chondrocytes were transfected with short hairpin RNAs specifically targeting Fxyd5 to silence its expression. Subsequently, cells were induced with lipopolysaccharide (LPS). The protein expression levels of Fxyd5, MMPs and proteins related to ECM, apoptosis and NF­κB signaling were detected using western blot analysis. In addition, cell viability was assessed using a Cell Counting Kit­8 assay, while the secretion of the proinflammatory factors and those of the oxidative stress­related markers were measured using the corresponding kits. Finally, cells were treated with the NF­κB activator, betulinic acid (BA) and the above experiments were repeated. The results demonstrated that Fxyd5 was significantly upregulated in ATDC5 cells treated with LPS. Additionally, Fxyd5 knockdown increased cell viability, enhanced the protein expression of Bcl­2, Aggrecan and collagen II, while reduced the expression of Bax, cleaved caspase­3/caspase­3, MMP3 and MMP13 in LPS­induced ATDC5 cells. The production of IL­1ß, IL­6 and IL­18 as well as reactive oxygen species and malondialdehyde, and the reduction of superoxide dismutase caused by LPS in ATDC5 cells, were also reversed by Fxyd5 silencing. Fxyd5 silencing inhibited the phosphorylation of p65 and IκBα induced by LPS. Finally, BA reversed the protective effect of Fxyd5 silencing on LPS induced chondrocytes injury. In conclusion, Fxyd5 could enhance chondrocyte inflammation and ECM degradation via activating the NF­κB signaling.


Assuntos
Condrócitos , Osteoartrite , Animais , Apoptose , Condrócitos/metabolismo , Matriz Extracelular/metabolismo , Inflamação/metabolismo , Lipopolissacarídeos/efeitos adversos , Camundongos , NF-kappa B/metabolismo , Osteoartrite/genética , Osteoartrite/metabolismo
14.
ACS Appl Mater Interfaces ; 14(7): 9073-9083, 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35138796

RESUMO

Atomically dispersed nitrogen-coordinated transition-metal sites supported on graphene (TM-N4-C) offer promising potential for the electrochemical carbon dioxide reduction reaction (CO2RR). However, a few TM-Nx-C single-atom catalysts (SAC) are capable of reducing CO2 to multielectron products with high activity and selectivity. Herein, using density functional theory calculations, we investigated the electrocatalytic performance of a single TM atom embedded into a defective BCN nanosheet for CO2RR. The N and B atom co-coordinated TM center, namely, TM-B2N2, constructs a symmetry-breaking site, which strengthens the overlapping of atomic orbitals, and enables the linear CO2 to be curved and activated, compared to the weak coupling of CO2 with the symmetric TM-N4 site. Moreover, the TM-B2N2 sites play a role of dual-atom active sites, in which the TM atom serves as the carbon adsorption site and the B atom acts as the oxygen adsorption site, largely stabilizing the key intermediates, especially *COOH. The symmetry-breaking coordination structures shift the d-band center of the TM atom toward the Fermi level and thus facilitate CO2 reduction to hydrocarbons and oxygenates. As a result, different from the TM-N4-C structure that leads to CO as the major product, the Ni atom supported on BCN can selectively catalyze CO2 conversion into CH4, with an ultralow limiting potential of -0.07 V, while suppressing the hydrogen evolution reaction. Our finding suggests that introduction of a nonmetal active site adjacent to the metal site provides a new avenue for achieving efficient multi-intermediate electrocatalytic reactions.

15.
Nano Lett ; 22(4): 1573-1579, 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35148110

RESUMO

Two-dimensional (2D) metal-organic frameworks (MOFs) with room-temperature magnetism are highly desirable but challenging due to the weak superexchange interaction between metal atoms. For this purpose, strengthening the hybridization between metal ion and organic linkage presents an experiment-feasible chemical solution to enhance the Curie temperature. Here, we report three 2D Cr(II) aromatic heterocyclic MOF magnets with enhanced Curie temperature by bridging Cr(II) ions with pyrazine, 1,4-diphosphinine, and 1,4-diarsenin linkers, i.e., Cr(pyz)2, Cr(diphos)2, and Cr(diarse)2, and using first-principles calculations. Our results show that Cr(pyz)2, Cr(diphos)2, and Cr(diarse)2 are ferrimagnetic semiconductors. In particular, the Curie temperature of Cr(pyz)2 is estimated to be about 344 K and could be enhanced to 512 and 437 K in Cr(diphos)2 and Cr(diarse)2 by strengthening the hybridization between Cr ions and organic linkers via d-π* direct exchange interaction. This study presents a prototype to obtain room-temperature magnetism in 2D Cr(II)-based MOF magnets for nanoscale spintronics applications.

16.
J Psychiatr Res ; 147: 254-261, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35074741

RESUMO

OBJECTIVE: Depression disorder is accompanied by cognitive impairments. However, there is limited research focused on cognitive impairments and their neurological mechanism in adolescents with depression. The purpose of the current study is to illustrate the differences in brain activity patterns between depressed adolescents and healthy controls (HCs). METHOD: A total of 72 adolescents with depression, as well as 74 HCs, were recruited. We utilized functional near-infrared spectroscopy (fNIRS) to monitor the concentrations of oxyhemoglobin (Oxy-Hb) in the brains of participants while they performed the verbal fluency task (VFT) to examine cognitive impairment in adolescents with depression. RESULTS: Our study demonstrated that adolescents with depression had significantly less cortical activation in the hemodynamic responses of Oxy-Hb at channels mainly located in the prefrontal cortex (PFC) than HCs during the 60-s task period (false discovery rate (FDR)-corrected p < 0.05). The mean channel-to-channel connectivity was 0.400 for HCs (SD = 0.149) and 0.303 (SD = 0.138) for adolescents with depression, and the HC group had a higher mean channel-to-channel connectivity strength than the depression group (t = -15.586, p < 0.001). For the patient group, we found significant negative correlations between HAMD scores and mean Oxy-Hb changes in Channel 38 (r = -0.33, p < 0.01), Channel 39 (r = -0.34, p < 0.01), Channel 41 (r = -0.25, p < 0.05), Channel 42 (r = -0.28, p < 0.05), and Channel 44 (r = -0.27, p < 0.05), and these channels were mainly located in areas with little difference between groups. CONCLUSIONS: Our study provides neurological evidence about the executive function (EF) in depressed adolescents. Adolescents with depression exhibited an abnormal activation pattern and decreased task-related functional connectivity compared to HCs. The changed Oxy-Hb concentration of PFC during VFT was not sensitive to depression symptoms.


Assuntos
Disfunção Cognitiva , Depressão , Adolescente , Disfunção Cognitiva/diagnóstico por imagem , Disfunção Cognitiva/etiologia , Humanos , Testes Neuropsicológicos , Oxiemoglobinas/metabolismo , Córtex Pré-Frontal/metabolismo , Espectroscopia de Luz Próxima ao Infravermelho/métodos
17.
Plant Physiol ; 188(4): 2115-2130, 2022 03 28.
Artigo em Inglês | MEDLINE | ID: mdl-35022793

RESUMO

The common ancestor of seed plants and mosses contained homo-oligomeric cellulose synthesis complexes (CSCs) composed of identical subunits encoded by a single CELLULOSE SYNTHASE (CESA) gene. Seed plants use different CESA isoforms for primary and secondary cell wall deposition. Both primary and secondary CESAs form hetero-oligomeric CSCs that assemble and function in planta only when all the required isoforms are present. The moss Physcomitrium (Physcomitrella) patens has seven CESA genes that can be grouped into two functionally and phylogenetically distinct classes. Previously, we showed that PpCESA3 and/or PpCESA8 (class A) together with PpCESA6 and/or PpCESA7 (class B) form obligate hetero-oligomeric complexes required for normal secondary cell wall deposition. Here, we show that gametophore morphogenesis requires a member of class A, PpCESA5, and is sustained in the absence of other PpCESA isoforms. PpCESA5 also differs from the other class A PpCESAs as it is able to self-interact and does not co-immunoprecipitate with other PpCESA isoforms. These results are consistent with the hypothesis that homo-oligomeric CSCs containing only PpCESA5 subunits synthesize cellulose required for gametophore morphogenesis. Analysis of mutant phenotypes also revealed that, like secondary cell wall deposition, normal protonemal tip growth requires class B isoforms (PpCESA4 or PpCESA10), along with a class A partner (PpCESA3, PpCESA5, or PpCESA8). Thus, P. patens contains both homo-oligomeric and hetero-oligomeric CSCs.


Assuntos
Briófitas , Bryopsida , Bryopsida/genética , Parede Celular , Celulose , Glucosiltransferases/genética , Sementes
18.
Chin J Integr Med ; 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35015223

RESUMO

In conclusion, this case should remind clinicians of the potential dangers of glycyrrhizin (high blood pressure, low blood potassium and abnormal changes in ECG, among other effects). The timely detection and effective treatment of glycyrrhizin-induced PsHA can prevent the occurrence of serious complications. The importance of taking a detailed medical history is also emphasized.

20.
New Phytol ; 233(3): 1238-1256, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34767648

RESUMO

Recent studies have revealed the physiological roles of glutamate receptor-like channels (GLRs) in Arabidopsis; however, the functions of GLRs in rice remain largely unknown. Here, we show that knockout of OsGLR3.4 in rice leads to brassinosteroid (BR)-regulated growth defects and reduced BR sensitivity. Electrophoretic mobility shift assays and transient transactivation assays indicated that OsGLR3.4 is the downstream target of OsBZR1. Further, agonist profile assays showed that multiple amino acids can trigger transient Ca2+ influx in an OsGLR3.4-dependent manner, indicating that OsGLR3.4 is a Ca2+ -permeable channel. Meanwhile, the study of internode cells demonstrated that OsGLR3.4-mediated Ca2+ flux is required for actin filament organization and vesicle trafficking. Following root injury, the triggering of both slow wave potentials (SWPs) in leaves and the jasmonic acid (JA) response are impaired in osglr3.4 mutants, indicating that OsGLR3.4 is required for root-to-shoot systemic wound signaling in rice. Brassinosteroid treatment enhanced SWPs and OsJAZ8 expression in root-wounded plants, suggesting that BR signaling synergistically regulates the OsGLR3.4-mediated systemic wound response. In summary, this article describes a mechanism of OsGLR3.4-mediated cell elongation and long-distance systemic wound signaling in plants and provides new insights into the contribution of GLRs to plant growth and responses to mechanical wounding.


Assuntos
Oryza , Brassinosteroides/metabolismo , Brassinosteroides/farmacologia , Regulação da Expressão Gênica de Plantas , Oryza/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Receptores de Glutamato/genética , Receptores de Glutamato/metabolismo
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