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1.
BMC Cancer ; 20(1): 448, 2020 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-32429931

RESUMO

BACKGROUND: Circular RNAs (circRNAs) are research hotspots in the network of noncoding RNAs in numerous tumours. The purpose of our study was to evaluate the clinicopathological, prognostic and diagnostic value of circRNAs in colorectal cancer. METHODS: The PubMed, Cochrane Library, and Web of Science online databases were searched for relevant studies before May 15, 2019. Pooled hazard ratios (HRs) and odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association between circRNAs expression, and overall survival (OS) and clinical parameters. Pooled sensitivity, specificity, and the area under the curve (AUC) were employed to assess the diagnostic value of circRNAs. RESULTS: A total of 19 studies were enrolled in this meta-analysis, with 11 on clinicopathological parameters, 8 on prognosis and 7 on diagnosis. For clinicopathological and prognostic value, elevated expression of oncogenic circRNAs was correlated with poor clinical parameters (tumor size: OR = 1.769, 95% CI: 1.097-2.852; differentiation grade: OR = 1.743, 95% CI: 1.032-2.946; TNM stage: OR = 3.320, 95% CI: 1.529-7.207; T classification: OR = 3.410, 95% CI: 2.088-5.567; lymph node metastasis: OR = 3.357, 95% CI: 2.160-5.215; distal metastasis: OR = 4.338, 95% CI: 2.503-7.520) and worse prognosis (HR = 2.29, 95% CI: 1.50-3.52). However, elevated expression of tumor-suppressor circRNAs was correlated with better clinical parameters (differentiation grade: OR = 0.453, 95% CI: 0.261-0.787; T classification: OR = 0.553, 95% CI: 0.328-0.934; distal metastasis: OR = 0.196, 95% CI: 0.077-0.498) and favorable prognosis (HR = 0.37, 95% CI: 0.22-0.64). For diagnostic value, the pooled sensitivity, specificity, and AUC were 0.82 (95% CI, 0.75-0.88), 0.72 (95% CI, 0.66-0.78), and 0.82 (95% CI, 0.78-0.85), respectively. CONCLUSIONS: These results indicate that circRNAs may be potential biomarkers for the diagnosis and prognosis of colorectal cancer.

2.
Cell Biol Int ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32379372

RESUMO

Transmembrane protein 98 (TMEM98) is a recently discovered gene, the inhibition of which has preliminarily been demonstrated to inhibit progression of several solid cancers in vitro. However, its involvement in tumorigenesis of gastric cancer (GC) has not been reported. Here, we aimed to explore the expression of TMEM98 in GC tissues and cell lines and to determine the role of TMEM98 in GC cell proliferation and invasion. TMEM98 was significantly upregulated in GC tissues, which was associated with low survival rate of GC patients. Interestingly, GC cell proliferation and invasion were promoted by TMEM98 messenger RNA (mRNA) upregulation and inhibited by TMEM98 mRNA downregulation, but not affected by TMEM98 protein. Using RNA-binding protein immunoprecipitation assay and RNA pull-down assay, we demonstrated that TMEM98 mRNA could directly bind with and upregulate nuclear factor 90 (NF90). Similarly, NF90 protein could not only enhance the stability of TMEM98 mRNA but antagonize the suppressive effect of TMEM98-small interfering RNA on proliferation and invasion in MKN-45 cells. Moreover, RNA pull-down assay, with wild-type (WT) and binding-site-mutated biotinylated TMEM98 mRNA transcripts, demonstrated that WT TMEM98 mRNA bound with NF90 protein through an 8-nt motif at the last exon, but the motif mutation abolished the capacity of TMEM98 mRNA binding to NF90 protein. Furthermore, overexpression of the WT last exon of TMEM98 increased NF90 expression and cell proliferation/invasion expectedly, but overexpression of the mutated last exon had no obvious effect. In conclusion, TMEM98 mRNA enhanced the proliferation and invasion of GC cells by interacting with the NF90 protein.

3.
Technol Cancer Res Treat ; 19: 1533033820920971, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32356487

RESUMO

OBJECTIVE: Colorectal cancer is one of the most important malignant cancer in the world with high incidence and mortality. Some studies have found that the expression of low serum L1 cell adhesion molecule is associated with poor prognosis in some malignancies. It is suggested that L1 cell adhesion molecule is a candidate serum marker for certain tumors. However, the relationship between serum L1 cell adhesion molecule and colorectal cancer, especially about the diagnostic value, is rarely reported. Therefore, this study aimed to evaluate the diagnostic potential of serum L1 cell adhesion molecule in patients with colorectal cancer. METHODS: Enzyme-linked immunosorbent assay was carried out to detect L1 cell adhesion molecule level in sera of 229 patients with colorectal cancer and 145 normal controls. Receiver operating characteristic curves were employed to calculate the accuracy of diagnosis. RESULTS: The levels of serum L1 cell adhesion molecule in the colorectal cancer group were significantly lower than that in normal controls (P < .05). In the normal group, the area under the receiver operating characteristic curve (area under the curve) of all colorectal cancer was 0.781 (95% confidence interval: 0.734-0.828) and early-stage colorectal cancer was 0.764 (95% confidence interval: 0.705-0.823). With optimized cutoff of 17.760 ng/mL, L1 cell adhesion molecule showed certain diagnostic value with specificity of 90.3% and sensitivities of 43.2% and 36.2% in colorectal cancer and early-stage colorectal cancer, respectively. Clinical data analysis showed that the levels of L1 cell adhesion molecule were significantly correlated with gender (P < .05) and early and late stages (P < .05). Furthermore, when compared with carcinoembryonic antigen, serum L1 cell adhesion molecule had significantly improved diagnostic accuracy for both colorectal cancer and early-stage colorectal cancer. CONCLUSIONS: Our study demonstrated that serum L1 cell adhesion molecule might be served as a potential biomarker for the diagnosis of colorectal cancer.

4.
Mediators Inflamm ; 2020: 8297134, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32454795

RESUMO

Purpose: To evaluate the regulating effect of Notch-Hes1 signaling on IL-17A+ γδ +T cell expression and IL-17A secretion in mouse psoriasis-like skin inflammation. Materials and Methods: Experimental mice were randomly divided into control group, model group (5% imiquimod- (IMQ-) treated mice), and intervention group (IMQ and γ-secretase inhibitor DAPT cotreated mice). The severity of psoriasis-like skin inflammation was evaluated by target lesion score based on the clinical psoriasis area and severity index (PASI). Flow cytometry detected IL-17A+ γδ +T cell percentage. Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. Additionally, splenic single cells from model mice were treated by DAPT to further evaluate the inhibitory effect of blocking Notch-Hes1 signaling on IL-17A+ γδ +T cell differentiation and IL-17A secretion. Results: The spleen index, IL-17A+ γδ +T cell percentage, Hes1 mRNA expression, IL-17A serum concentration, and protein expression were all significantly higher in model mice than control mice, while dramatically reduced in intervention mice by DAPT treatment, which also obviously alleviated the target lesion score, epidermal hyperplasia, and dermal inflammatory cell infiltration of intervention mice. In vitro study demonstrated that DAPT treatment could result in dose-dependent decrease of IL-17A+ γδ +T cell percentage and IL-17A secretion in splenic single cells of model mice.

5.
Langmuir ; 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32460491

RESUMO

The development of new routes or materials to realize superlubricity under high contact pressure can result in energy-saving and reduction of emissions. In this study, superlubricity (µ = 0.0017) under extreme pressure (717 MPa, more than twice the previously reported liquid superlubricity) between the frictional pair of Si3N4/sapphire was achieved by pre-running-in with a H3PO4 (HP) solution followed by lubrication with an aqueous solution consisting of polyvinyl alcohol (PVA) and sodium chloride (NaCl). Under the same test condition, the aqueous PVA lubricant did not show superlubricity. Results of X-ray photoelectron spectroscopy and Raman spectroscopy indicate the formation of a PVA-adsorbed film at the frictional interface after lubrication with PVA but not after lubrication with PVA/NaCl, indicating competitive adsorption between hydrated Na+ ions and PVA molecules. The hydrated Na+ ions adsorbed preferentially to the solid surfaces, causing the transformation of the shear interface from a polymer film/polymer film to a solid/polymer film. Meanwhile, the hydrated Na+ ions also produced hydration repulsion force and induced low shear stress between the solid surfaces. Furthermore, NaCl increased the viscosity of the polymer lubricant, enhanced the hydrodynamic effect between interfaces, and decreased direct contact between the friction pair, causing a further reduction in friction. Thus, the superlubricity of the PVA/NaCl mixture is attributed to the combination of hydration and hydrodynamic effects. This study provides a novel route and mechanism for achieving extreme-pressure superlubricity at the macroscale, through the synergistic lubricating effect of hydrated ions and a polymer solution, propelling the industrial application of superlubricity.

6.
Vaccine ; 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32417139

RESUMO

Classical swine fever (CSF) remains one of the most important highly contagious and fatal viral disease of swine with high morbidity and mortality. CSF is caused by classical swine fever virus (CSFV), a small, enveloped RNA virus of the genus Pestivirus. The aim of this study was to construct the a novel CSFV Fc-fusion recombinant protein and evaluate the efficacy as a vaccine against CSFV. Here, we obtained a novel subunit vaccine expressing CSFV E2 recombinant fusion protein in CHO-S cells. Functional analysis revealed that CSFV Fc-fusion recombinant protein (CSFV-E2-Fc) could bind to FcγRI on antigen-presenting cells (APCs) and significantly increase IgA levels in serum and feces, inducing stronger mucosal immune response in swine. Additionally, CSFV-E2-Fc immunization enhanced CSFV-specific T cell immune response with a Th1-like pattern of cytokine secretion, remarkably stimulated the Th1-biased cellular immune response and humoral immune response. Further, the protective effects of CSFV-E2-Fc subunit vaccines were confirmed. The data suggest that CSFV E2-Fc recombinant fusion protein may be a promising candidate subunit vaccine to elicit immune response and protect against CSFV.

7.
J Clin Lab Anal ; : e23366, 2020 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-32419252

RESUMO

BACKGROUND: Monocytes are recruited into the cerebrospinal fluid (CSF) of patients with neurosyphilis, suggesting abnormal chemokine expression. We aimed to investigate the aberrant expression of chemokines in the CSF of these patients. METHODS: CSF and serum samples were collected from patients with neurosyphilis between July 2017 and June 2019 in the Dermatology Department, Second Affiliated Hospital of Zhejiang University. Differences in the expression of 38 chemokines between patients with and without neurosyphilis were detected using RayBio® Human Chemokine Antibody Array C1. CCL24 and CXCL7 levels in the patients' CSF and serum were further measured using RayBio® CCL24 and CXCL7 ELISA kits. RESULTS: Ninety-three CSF and serum samples of patients with syphilis were collected. Antibody array analysis showed that the CSF levels of CCL24 (P = .0185), CXCL7 (P < .0001), CXCL13 (P < .0001), CXCL10 (P < .0001), and CXCL8 (P < .0001) were significantly higher in patients with than without neurosyphilis. ELISA confirmed significantly higher CCL24 and CXCL7 levels in the CSF of patients with than without neurosyphilis (CCL24: 6.082 ± 1.137 pg/mL vs 1.773 ± 0.4565 pg/mL, P = .0037; CXCL7: 664.3 ± 73.19 pg/mL vs 431.1 ± 90.54 pg/mL, P = .0118). Increased CCL24 and CXCL7 expression was seen throughout all neurosyphilis stages, had moderate diagnostic efficiency for neurosyphilis, and correlated poorly with CSF cell count and Venereal Disease Research Laboratory titer. CSF CCL24 levels also correlated poorly with CSF protein concentration. CONCLUSION: Abnormally high CSF chemokines levels may play a role in the pathogenesis of neurosyphilis.

8.
Int J Biol Macromol ; 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32439447

RESUMO

This study aimed to determine the potential of sodium starch octenylsuccinate (SSOS)-based Pickering emulsions incorporated with cinnamon essential oil (CEO) as a vehicle for delivering active biodegradable films to improve their antimicrobial and antioxidant activites. This study explored the combined effects of mixed oils (CEO and corn oils). Particle size distribution, mean drop size, ζ-potential, polymer dispersity index (PDI), and microstructure were determined to confirm emulsion stability. Well-distributed emulsion droplets maintained under a good condition and encapsulated by SSOS resulted in homogeneous and stable emulsions. The obtained mixed oil-loaded Pickering emulsions were incorporated into the SSOS film preparation, and the morphological, physical, and functional characteristics of the active film were studied. An optimal CEO/corn oil ratio decreased tensile strength but improved elongation at break, water vapor permeability, and oxygen permeability. Fourier transform infrared spectroscopy and scanning electron microscopy showed that the SSOS membrane matrix is compatible with Pickering emulsions incorporated with mixed oil. Moreover, a significant decrease was observed in loss of CEO by the Pickering emulsion method. This method exhibited antioxidant and antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, indicating that the encapsulation strategy was efficient to protect against loss of CEO during drying and storage.

9.
Biosci Rep ; 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32436962

RESUMO

MiR-33a is found as a regulator of cell proliferation in many cancer cells. However, it remains unknown if and how miR-33a plays a role in myoblasts proliferation. To investigate the effect of miR-33a on myoblasts proliferation, miR-33a mimic or inhibitor was co-administered with or without insulin-like growth factor 1 (IGF1) to simulation myoblasts. Our study showed that upregulation of miR-33a impaired myoblasts proliferation, while downregulation of miR-33a enhanced myoblasts proliferation. Mechanistically, we examined that miR-33a can inhibit the transcription of IGF1, follistatin (FST) and cyclin D1 (CCND1) by targeting their 3'UTR region in both HEK293T cells and duck myoblasts. Moreover, upregulation of miR-33a decreased and its downregulation increased the mRNA expression of PI3K, Akt, mTOR and S6K. Importantly, the decreased PI3K, Akt, mTOR and S6K expression by miR-33a mimics was abrogated by co-administered with IGF1. Altogether, our results demonstrated that miR-33a may directly target IGF1, FST and CCND1 to inhibit myoblasts proliferation via PI3K/Akt/mTOR signaling. In conclusion, miR-33a is a potential negative regulator of myoblasts proliferation and by modulating its expression. miR-33a could play an important role in promoting the early development of skeletal muscle.

10.
Sci Rep ; 10(1): 6019, 2020 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-32265473

RESUMO

Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) is an important issue associated with significant morbidity and mortality and well known as a predominant pathogen causing bloodstream infection (BSIs) globally. To estimate the antibiotic resistance and molecular characteristics of S. aureus causing BSIs in Shanghai, 120 S. aureus isolates (20 isolates each year) from the patients with S. aureus BSIs from 2013 to 2018 were randomly selected and enrolled in this study. Fifty-three (44.2%) MRSA isolates were determined, and no isolate was found resistant to vancomycin, daptomycin, synercid, linezolid and ceftaroline. The toxin genes tst, sec, seg and sei were found more frequently among MRSA isolates compared with MSSA isolates (all P < 0.0001). Twenty-nine sequence types (STs) were identified, and ST5 (23.3%) was the most common ST, followed by ST398 (11.7%) and ST764 (10.0%). SCCmec II (73.6%) was the most frequent SCCmec type among MRSA isolates. The dominant clonal complexes (CCs) were CC5 (ST5, ST764, ST965 and ST3066; 36.7%) and the livestock-associated clone CC398 (ST398, 11.7%). MRSA-CC5 was the predominant CC among MRSA isolates (37/53, 69.8%), and CC5-II MRSA was found in 34 isolates accounting for 91.9% (34/37) among CC5 MRSA isolates. In addition, all 29 tst-positive MRSA isolates were CC5-MRSA as well. Our study provided the properties and genotypes of S. aureus causing BSIs at Ruijin Hospital in Shanghai from 2013 to 2018, and might suggest of value clues for the further study insights into pathogenic mechanisms intrinsically referring to the development of human-adapted S. aureus clones and their diffusions.

11.
Soft Matter ; 16(16): 4024-4031, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32270152

RESUMO

The gemini-like surfactants have been constructed by compounding N-erucamidopropyl-N,N-dimethylamine (UC22AMPM) and o-phthalic acid (o-PA), m-phthalic acid (m-PA), or p-phthalic acid (p-PA), and are denoted as o-EAPA, m-EAPA, and p-EAPA, respectively. It is well known that inorganic salts have significant effects on surfactant aggregates, and herein the effects of sodium chloride (NaCl) on gemini-like surfactants is explored by rheological and dynamic light scattering measurements, and cryo-TEM. It is found that the viscoelasticity of the EAPA systems first increases and then decreases with an increase of the NaCl concentration. And the optimal NaCl concentrations for these three systems are in the order of o-EAPA < m-EAPA < p-EAPA due to different spacer distances between the two carboxyl groups in the phthalic acid. Similar trends in the N,N-dimethyl oleoaminde-propylamine (DOAPA) and o-PA, m-PA, or p-PA systems were also observed. The results show that an appropriate NaCl concentration will promote gemini-like surfactants to form wormlike micelles (WLMs). Upon further increasing the NaCl concentration, the WLMs transform into vesicles. Excessive NaCl concentration will cause the surfactant systems to reach their cloud point and make the surfactants precipitate out. The mechanism of the effects of NaCl is that Cl- reduces the electrostatic repulsion between the headgroups of the surfactants. This work is helpful in understanding the effects of inorganic salts on the surfactants and this study is useful for exploring the practical applications of gemini-like surfactants.

12.
Sensors (Basel) ; 20(8)2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32331277

RESUMO

This study reports an oven-controlled microelectromechanical systems oscillator with integrated micro-evaporation trimming that achieves frequency stability over the industrial temperature range and permanent frequency trimming after vacuum packaging. The length-extensional-mode resonator is micro-oven controlled and doped degenerately with phosphorous to achieve a frequency instability of ±2.6 parts per million (ppm) in a temperature range of -40 to 85 °C. The micro-evaporators are bonded to the resonator, integrated face-to-face, and encapsulated in vacuum. During trimming, the micro-evaporators are heated electrically, and the aluminum layers on their surfaces are evaporated and deposited on the surface of the resonator that trims the resonant frequency of the resonator permanently. The impact of the frequency trimming on the temperature stability is very small. The temperature drift increases from ±2.6 ppm within the industrial temperature range before trimming to ±3.3 ppm after a permanent trimming of -426 ppm based on the local evaporation of Al. The trimming rate can be controlled by electric power. A resonator is coarse-trimmed by approximately -807 ppm with an evaporation power of 960 mW for 0.5 h, and fine-trimmed by approximately -815 ppm with an evaporation power of 456 mW for 1 h. Though the Q-factor decreases after trimming, a Q-factor of 304,240 is achieved after the trimming of -1442 ppm.

13.
Bioresour Technol ; 309: 123346, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32305016

RESUMO

Three-dimensional electrode biological aerated filter (3DE-BAF) with particulate bioelectrode from lithium slag was used to simultaneously remove diclofenac and clofibric acid from the synthetic domestic sewage, and the combined effects of electrical current and nonsteroidal antiinflammatory drugs (NSAIDs) on microbial community was analyzed. The results indicated that (1) the average diclofenac and clofibric acid removal efficiency in the 3DE-BAF firstly increased, attained the peak of 79.40 ± 6.74% and 69.50 ± 6.26% at 0.35 A, and then decreased to 71.82 ± 4.90% and 55.92 ± 5.17% at 0.40 A, respectively; (2) the concentration of the diclofenac and clofibric acid in 3DE-BAF gradually decreased with the increase of reactor height; (3) the current intensity and space position affected the microbial structure at the different level; (4) at the optimum current intensity, Thiothrix, Flavobacteriaceae, Halothiobacillaceae, Hydrogenophaga, and Comamonadaceae accounted for the main bacterial community for removal diclofenac and clofibric acid in the 3DE-BAF.


Assuntos
Microbiota , Anti-Inflamatórios não Esteroides , Reatores Biológicos , Diclofenaco , Eletrodos , Filtração , Esgotos , Eliminação de Resíduos Líquidos
14.
Chem Asian J ; 15(9): 1464-1468, 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32227593

RESUMO

GSTP1 has been considered to be a marker for malignancy in many tissues. However, the existing GST fluorescent probes are unfavorable for in vivo imaging because of the limited emission wavelength or insufficient fluorescence enhancement (six-fold). The limited fluorescence enhancement of GST fluorescent probes is mainly ascribed to the high background signals resulting from the spontaneous reaction between GSH and the probes. In this work, a highly specific GST probe with NIR emission has been successfully developed through optimization of the essential unit of the probe to repress the spontaneous reaction. The novel GST probe exhibits over 100-fold fluorescence enhancement upon incubation with GSTP1/GSH and high selectivity over other potential interference. In addition, the probe has been proved to be capable of tracking endogenous GST in A549 cells. Finally, the in vivo imaging results demonstrate that the probe can be used for effective imaging of endogenous GST activity in subcutaneous tumor mouse with high contrast.

15.
Viruses ; 12(4)2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32230737

RESUMO

Pseudorabies (PR), caused by pseudorabies virus (PRV), is an acute and febrile infectious disease in swine. To eradicate PR, a more efficacious vaccine needs to be developed. Here, the gE/gI- and TK/gE/gI-gene-deleted recombinant PRV (rGXΔgE/gI and rGXΔTK/gE/gI) are constructed through CRISPR/Cas9 and Cre/Lox systems. We found that the rGXΔTK/gE/gI was safer than rGXΔgE/gI in mice. Additionally, the effects of rGXΔgE/gI and rGXΔTK/gE/gI were further evaluated in swine. The rGXΔgE/gI and rGXΔTK/gE/gI significantly increased numbers of IFN-γ-producing CD4+ and CD8+ T-cells in swine, whereas there was no difference between rGXΔgE/gI and rGXΔTK/gE/gI. Moreover, rGXΔgE/gI and rGXΔTK/gE/gI promoted a PRV-specific humoral immune response. The PRV-specific humoral immune response induced by rGXΔgE/gI was consistent with that caused by rGXΔTK/gE/gI. After the challenge, swine vaccinated with rGXΔgE/gI and rGXΔTK/gE/gI showed no clinical signs and viral shedding. However, histopathological detection revealed that rGXΔgE/gI, not rGXΔTK/gE/gI, caused pathological lesions in brain and lung tissues. In summary, these results demonstrate that the TK/gE/gI-gene-deleted recombinant PRV was safer compared with rGXΔgE/gI in swine. The data imply that the TK/gE/gI-gene-deleted recombinant PRV may be a more efficacious vaccine candidate for the prevention of PR.

16.
J Hazard Mater ; 395: 122676, 2020 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-32325342

RESUMO

Lead (Pb) is a typical hazardous element of high concern in species characteristics involving toxicity, migration and transformation. A greenhouse experiment was conducted using Solanum nigrum L. grown in soils treated by divalent (Pb2+), tetravalent (Pb4+), trimethyl (TML) and triethyl (TEL) lead for 60 days. Results of physio-biochemical parameters indicated Pb toxicity was ranked as TEL > TML > Pb2+ > Pb4+ in a dose-dependent manner, and the correlation levels of organic species were higher than inorganic species. S. nigrum L. adopted phytostabilization strategy through fixing Pb in roots and restricting its transfer to shoots. More phytotoxic Pb was absorbed from soils treated by Pb2+ than Pb4+ as well as TEL than TML. In soils, inorganic Pb species were mainly present in residues while organic Pb species in Fe/Mn oxide and exchangeable fractions. Although most of Pb species in plant existed in the low-bioavailable extractions of 1 M NaCl and 2% HAC, the water-soluble Pb extracted by d-H2O and 80 % ethanol were increased to a large extent under high-level exposure. The occurrence of reduction and (de)alkylation were considered as the major pathways in the biotransformation of Pb species. This study will conduce to the ecological risk management for Pb-contaminated soils.

17.
Small ; 16(18): e2000307, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32250065

RESUMO

Alpha-methylacyl-CoA racemase (AMACR) has been proven to be consistently overexpressed in prostate cancer epitheliums, and is expected to act as a positive biomarker for the diagnosis of prostate carcinoma in clinical practice. Here, a strategy for specific determination of AMACR in real human serum by using an electrochemical microsensor system is presented. In order to implement the protocol, a self-organized nanohybrid consisting of metal nanopillars in a 2D MoS2 matrix is developed as material for the sensing interface. The testing signal outputs are strongly enhanced with the presence of the nanohybrids owing to that the metal pillars provide an efficient mass difussion and electron transfer path to the MoS2 film surface. Furthermore, the phase-regulated sensing mechanism over MoS2 is noticed and demonstrated by density functional theory calculation and experiments. The explored MoS2 based nanohybrids are employed for the fabrication of an electrochemical microsensor, presenting good linear relationship in both ng µL-1 and pg µL-1 ranges for AMACR quantification. The sampling analysis of human serum indicates that this microsensor has good diagnostic specificity and sensitivity toward AMACR. The proposed electrochemical microsensor system also demonstrates the advantages of convenience, cost-effectiveness, and disposability, resulting in a potential integrated microsystem for point-of-care prostate cancer diagnosis.

18.
Ann Bot ; 2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32297921

RESUMO

BACKGROUND AND AIMS: Efficient biological nitrogen fixation (BNF) requires leghaemoglobin (Lb) to modulate oxygen pressure in nodules. Excess N supply severely inhibits BNF through effects on Lb during nodulation. As yet, a systematic identification and characterization of Lb-encoding genes in soybean has not been reported. METHODS: The effects of N on BNF were studied in soybean plants inoculated with rhizobia and exposed to excess or low N availability in hydroponic cultures. To identify soybean Lb proteins, BLAST searches were performed on the Phytozome website. Bioinformatic analysis of identified GmLbs was then carried out to investigate gene structure, protein homology and phylogenetic relationships. Finally, quantitative real-time PCR was employed to analyse the expression patterns of soybean Lb genes in various tissues and in response to high N availability. KEY RESULTS: Excess N significantly accelerated nodule senescence and the production of green Lb in nodules. In total, seven haemoglobin (Hb) genes were identified from the soybean genome, with these Hb genes readily split into two distinct clades containing predominantly symbiosis-associated or non-symbiotic Hb members. Expression analysis revealed that all of the symbiosis-associated Lbs except GmLb5 were specifically expressed in nodules, while the non-symbiotic GmHbs, GmHb1 and GmHb2, were predominantly expressed in leaves and roots, respectively. Among identified GmLbs, GmLb1-4 are the major Lb genes acting in soybean nodulation, and each one is also significantly suppressed by exposure to excess N. CONCLUSIONS: Taken together, the results show that excess N inhibits BNF by reducing nodule formation, Lb concentration and nitrogenase activity. The characteristics of the entire Hb family were analysed, and we found that GmLb1-4 are closely associated with nodule development and N2 fixation. This works forms the basis for further investigations of the role of Lbs in soybean nodulation.

19.
Cardiology ; : 1-10, 2020 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-32305978

RESUMO

Ubiquitination is one of the basic mechanisms of cell protein homeostasis and degradation and is accomplished by 3 enzymes, E1, E2, and E3. Tripartite motif-containing proteins (TRIMs) constitute the largest subfamily of RING E3 ligases, with >70 current members in humans and mice. These members are involved in multiple biological processes, including growth, differentiation, and apoptosis as well as disease and tumorigenesis. Accumulating evidence has shown that many TRIM proteins are associated with various cardiac processes and pathologies, such as heart development, signal transduction, protein degradation, autophagy mediation, ion channel regulation, congenital heart disease, and cardiomyopathies. In this review, we provide an overview of the TRIM family and discuss its involvement in the regulation of cardiac proteostasis and pathophysiology and its potential therapeutic implications.

20.
J Clin Lab Anal ; : e23341, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32309888

RESUMO

BACKGROUND: Cancer has become a public health problem with high morbidity and mortality. Recent publications have shown that exosomes can be used as potential diagnostic biomarkers of cancer. However, the diagnostic accuracy and reliability of circulating exosomes remain unclear. The present meta-analysis was conducted to comprehensively summarize the overall diagnostic performance of circulating exosomes for cancer. METHODS: Eligible studies published up to June 27, 2019, on PubMed, Embase, and Cochrane Library were selected for the meta-analysis. All statistical analyses were performed by STATA 15.1 statistical software and Meta-DiSc 1.4. Quality Assessment for Studies of Diagnostic Accuracy 2 tool was used to access the quality of included studies. A bivariate mixed-effects model was applied to calculate the diagnostic indexes from included studies. RESULTS: A total of 5924 participants comprising 3161 cases and 2763 controls from 42 eligible studies were analyzed. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and the area under the curve with 95% confidence intervals (95% CI) were as follows: 0.79 (0.75-0.82), 0.81 (0.78-0.84), 4.1 (3.5-4.8), 0.26 (0.22-0.31), 16 (12-21), and 0.87 (0.84-0.89), respectively. Sensitivity analysis suggested no study exclusively contributed to the heterogeneity, and Deeks' funnel plot asymmetry test indicated no potential publication bias (P = .09). CONCLUSIONS: The meta-analysis indicated that circulating exosomes could serve as effective and minimally invasive biomarkers for diagnosis of cancer, especially in patients with hepatocellular carcinoma or ovarian cancer, serum-based samples and exosomal proteins.

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