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1.
Neural Plast ; 2020: 8842110, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33299396

RESUMO

Musculoskeletal pain (MSP) is one of the most severe complaints in women undergoing menopause. The prevalence of MSP varied when taking the menopausal state and age factor into consideration. This study investigated the prevalence of MSP in perimenopausal women and its association with menopausal state. The MEDLINE, Embase, Web of Science, and PubMed databases were searched from inception to July 2020, and 16 studies were retrieved for the current meta-analysis. The primary outcome measure was the MSP Odds Ratio (OR). The estimated overall prevalence of MSP among perimenopausal women was 71% (4144 out of 5836, 95% confidence interval (CI): 64%-78%). Perimenopausal women demonstrated a higher risk for MSP than premenopausal ones (OR: 1.63, 95% CI: 1.35-1.96, P = 0.008, I 2 = 59.7%), but similar to that in postmenopausal ones (OR: 1.07, 95% CI: 0.95-1.20, P = 0.316, I 2 = 13.4%). The postmenopausal women were at a higher risk of moderate/severe MSP than the premenopausal ones (OR: 1.45, 95% CI: 1.21-1.75, P = 0.302, I 2 = 16.5%) or the perimenopausal ones (OR: 1.40, 95% CI: 1.09-1.79, P = 0.106, I 2 = 55.4%). In conclusion, the perimenopause is a state during which women are particularly predisposed to develop MSP. As to moderate to severe degrees of MSP, the odds increase linearly with age, from premenopause to peri- and then to postmenopause.

2.
Org Lett ; 16(8): 2196-9, 2014 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-24697243

RESUMO

High contents of curcusones A and B and trace amounts of spirocurcasone exist in the roots of Jatropha curcas. Here, a one-step semisynthesis method of spirocurcasone and pyracurcasone was built, not only resulted an increased yield of spirocurcasone but also produced pyracurcasone, which exhibited greater cytotoxicity compared to curcusones A and B. The plausible mechanism of the formation of pyracurcasone was proposed, and the proposed biogenetic origin for spirocurcasone by Taglialatela-Scafati was confirmed.


Assuntos
Diterpenos/síntese química , Cisplatino/farmacologia , Diterpenos/química , Diterpenos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Células HL-60 , Humanos , Jatropha/química , Estrutura Molecular , Raízes de Plantas/química , Relação Estrutura-Atividade
3.
J Nat Prod ; 77(4): 737-43, 2014 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-24559087

RESUMO

Two novel trinorlanostanes, cochlates A and B (1 and 2), with a 3,4-seco-9,10-seco-9,19-cyclo skeleton, as well as six new triterpenoids, fornicatins D-F (3-5) and ganodercochlearins A-C (6-8), together with five known triterpenoids (9-13), were obtained from the fruiting bodies of Ganoderma cochlear. The structural elucidation was achieved by interpretation of spectroscopic data, and compounds 2 and 7a were further characterized by X-ray crystallographic analysis. Fornicatins A, D, and F (10, 3, and 5) and fredelin (13) lowered the ALT and AST levels in HepG2 cells treated with H2O2, suggesting that they could display in vivo hepatoprotective activities.


Assuntos
Ganoderma/química , Lanosterol/análogos & derivados , Lanosterol/isolamento & purificação , Lanosterol/farmacologia , Fígado/efeitos dos fármacos , Triterpenos/farmacologia , Algoritmos , China , Cristalografia por Raios X , Células Hep G2 , Humanos , Peróxido de Hidrogênio/análise , Lanosterol/química , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Triterpenos/química
4.
Phytochemistry ; 96: 265-72, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24079824

RESUMO

Twelve rhamnofolane diterpenoids, including curcusecons A-E with unusual seco-rhamnofolane skeletons, curcusones F-J, 4-epi-curcusone E, and 3-dehydroxy-2-epi-caniojane, together with seven known analogues, curcusones A-E, jatrogrossidione, and 2-epi-jatrogrossidione, were isolated from the roots of Jatropha curcas. Their structures were determined by extensive spectroscopic methods, and the relative stereochemistry of curcusecon B was further confirmed by X-ray crystallographic data. Their cytotoxity against five human cancer cells was studied and the results indicated that the dienone system in ring B was essential for cytotoxicity of these compounds.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Jatropha/química , Antineoplásicos Fitogênicos/química , Cristalografia por Raios X , Diterpenos/química , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Feminino , Células HL-60 , Humanos , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química , Relação Estrutura-Atividade
5.
Org Lett ; 15(7): 1580-3, 2013 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-23484960

RESUMO

Three novel norfriedelanes, A-C (1-3), were isolated from the branches and roots of Malpighia emarginata . Their structures and absolute configurations were determined by 1D and 2D NMR techniques and X-ray crystallographic analysis. Norfriedelin A (possessing an α-oxo-ß-lactone group) and norfriedelin B (with a keto-lactone group) showed acetylcholinesterase inhibitory effects with the IC50 values of 10.3 and 28.7 µM, respectively.


Assuntos
Inibidores da Colinesterase/isolamento & purificação , Inibidores da Colinesterase/farmacologia , Malpighiaceae/química , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , Inibidores da Colinesterase/química , Cristalografia por Raios X , Concentração Inibidora 50 , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Triterpenos/química
6.
Artigo em Chinês | MEDLINE | ID: mdl-20718346

RESUMO

OBJECTIVE: To clone and express VP, gene from HBoV, and the expressed VP, protein was as the antigen in order to detect serum from children in Wenling area with lower respiratory tract infections. METHODS: The VP, gene was recombined with the genome of Baculovirus, which infected the insect cell. The fusion protein with HA tag was applied to confirm the specificity of expressed protein. Furthermore, the recombinant protein was observed using electron microscopy. The 176 serum from children in Wenling area with lower respiratory tract infections was screened using Western blot. RESULTS: The expressed VP2 protein was more than 60% in total proteins from insect cell, and MWt about 60 x 10(3). The virus-like particle (VLP) was observed using electron microscopy, and size about 20 nm. The 176 serum from children in wenling area with lower respiratory tract infections was screened using Western blot. The HBoV positive rate was 2.28% (4/176). CONCLUSION: The VP2 protein from human bocavirus was expressed in insect cell successfully. Through HA tag the VP2 protein was specific, and then the assay using SDS-PAGE with Western blot could detect and screen the antibody in serum from children with lower respiratory tract infections rapidly and accurately.


Assuntos
Bocavirus/genética , Proteínas do Capsídeo/genética , Expressão Gênica , Infecções por Parvoviridae/diagnóstico , Animais , Anticorpos Antivirais/sangue , Bocavirus/imunologia , Proteínas do Capsídeo/imunologia , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Infecções por Parvoviridae/sangue , Infecções por Parvoviridae/imunologia , Infecções por Parvoviridae/virologia , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Spodoptera
7.
Artigo em Chinês | MEDLINE | ID: mdl-18574529

RESUMO

OBJECTIVE: In this study, human bronchial epithelial cells were inoculated with positive sputum specimens of HBoV. After four days' infection, cytopathic effects (CPE) were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout. These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical "owl's eye" plaque and above 90 percent hemadsorption within the infected cells by erythrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique. CONCLUSION: Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicity of human bocavirus.


Assuntos
Bocavirus/fisiologia , Células Epiteliais/virologia , Brônquios/citologia , Morte Celular/fisiologia , Sobrevivência Celular/fisiologia , Células Cultivadas , Células Epiteliais/citologia , Técnica Indireta de Fluorescência para Anticorpo , Interações Hospedeiro-Patógeno , Humanos , Microscopia de Fluorescência
8.
Bing Du Xue Bao ; 24(1): 69-71, 2008 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-18320826

RESUMO

WU polyomavirus, which was firstly discovered in 2007, is a new human polyomavirus belonging to Polyomaviridae and containing circular double-stranded genomic DNA. In this study, the 278 clinical sputum specimens from children under 5 years old were collected from Wenzhou Medical College affiliated Wenling First Hospital, Zhejiang Province. Based on identification assay of WU polyomavirus previously reported, a WU polyomavirus was identified from clinical samples successfully, the positive rate was 0.4%. The sequences of PCR products were identical to that of VP2 gene and large T antigen gene derived from WU polyomavirus reported. The above results strongly suggested that the WU polyomavirus isolated was firstly found in Chinese children with acute lower respiratory tract infections. This study provides a firm basis for further research of WU polyomavirus.


Assuntos
Polyomavirus/isolamento & purificação , Sequência de Bases , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polyomavirus/genética , Escarro/virologia
9.
Bing Du Xue Bao ; 23(1): 57-9, 2007 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-17886722

RESUMO

Human bocavirus, which was firstly discovered in 2005, is a new human parvovirus associated with lower respiratory tract infection in children. In this study, a human bocavirus, named WLL-1 isolate, was identified in Wenlin County, Zhejiang Province. The genome of bocavirus WLL-1 has been sequenced and analyzed. Phylogenetic analyses showed that WLL-1 shares 99% homology with other bocaviruses recently reported, but also has some special variations.


Assuntos
Bocavirus/genética , DNA Viral/genética , Genoma Viral , Bocavirus/classificação , Bocavirus/isolamento & purificação , China , DNA Viral/química , Humanos , Dados de Sequência Molecular , Filogenia , Análise de Sequência de DNA
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