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1.
Yi Chuan ; 41(12): 1110-1118, 2019 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-31857282

RESUMO

Myogenesis is a complex physiological process that is mainly involved in the proliferation of myogenic stem cells to form myoblasts, which then differentiated and fused to form multinucleated myotubes. Many proteins have been found to be involved in myoblast fusion, but none of them are muscle-specific fusion proteins. In recent years, two muscle-specific transmembrane proteins, i.e. Myomaker and Myomerger, have been discovered and identified, which can coordinate and promote the fusion of myoblasts and thus participate in the process of myogenesis. In this review, we summarize the research progress of Myomaker and Myomerger in myogenesis, including their expression patterns and functional domains, as well as their participation in myoblast fusion mechanisms, aiming to provide relevant ideas for in-depth study of the myogenesis process and treatment of diseases related to myoblast fusion.


Assuntos
Proteínas de Membrana , Músculo Esquelético , Mioblastos , Animais , Diferenciação Celular , Fusão Celular , Humanos , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Desenvolvimento Muscular , Proteínas Musculares , Músculo Esquelético/citologia , Mioblastos/citologia
2.
Int J Biol Macromol ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31756484

RESUMO

In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs). First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT). Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield. The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 °C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L. Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation. The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants. However, further structure elucidation and functional studies need to be continued.

3.
J Therm Biol ; 83: 165-171, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31331515

RESUMO

Temperature is a key factor that influences fish metabolism. Therefore, it is important to understand how temperature variation affects lipid metabolism and energy consumption in fish. We determined respiration frequency, Na+/K+-ATPase activity, and lipid metabolism in common carp (Cyprinus carpio) after 18 days of temperature stress (5 °C and 30 °C, with 17 °C as the control). We found that respiratory frequency was positively correlated with water temperature (p < 0.01), whereas red blood cell count and mean corpuscular hemoglobin content and concentration were negatively correlated with temperature. In liver and heart, triglyceride (TG) levels were lower in both stress groups (5 °C and 30 °C) than in the control (17 °C) (p < 0.01), and highest in muscle from the high temperature stress group (p < 0.01). The non-esterified fatty acid concentration was negatively correlated with TG levels. In brain, lipoprotein lipase (LPL) activity decreased with increasing temperature and hepatic lipase (HL) activity was lower in both stress groups than in the control (p < 0.01). In muscle, the activity of LPL and HL was lowest in the high temperature stress group, resulting in a significant increase in TG levels. Na+/K+-ATPase activity in heart was lower in both stress groups than in the control (p < 0.01). The expression of LPL mRNA in heart increased with increasing temperature (p < 0.01), whereas LPL mRNA expression in brain and liver increased in both stress groups (p < 0.01). Our results show that temperature can significantly affect lipid metabolism in common carp, and that different tissues respond differently to changes in temperature.


Assuntos
Carpas/fisiologia , Resposta ao Choque Frio , Proteínas de Peixes/metabolismo , Resposta ao Choque Térmico , Metabolismo dos Lipídeos , Respiração , ATPase Trocadora de Sódio-Potássio/metabolismo , Animais , Encéfalo/metabolismo , Carpas/genética , Carpas/metabolismo , Fígado/metabolismo , ATPase Trocadora de Sódio-Potássio/genética
4.
Front Aging Neurosci ; 11: 114, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31156421

RESUMO

Gestational infection causes various neurological deficits in offspring, such as age-related spatial learning and memory (SLM) decline. How inflammation causes age-related SLM dysfunction remains unknown. Previous research has indicated that histone modifications, such as phosphorylation of H3S10 (H3S10p) and trimethylation of H3K9 (H3K9me3) may be involved. In our study, pregnant mice received an intraperitoneal injection of lipopolysaccharide (LPS, 50 or 25 µg/kg) or normal saline during gestational days 15-17. After normal parturition, the offspring were randomly separated into 1-, 6-, 12-, 18-, and 22-month-old groups. SLM performance was assessed using a radial six-arm water maze (RAWM). The hippocampal levels of H3S10p and H3K9me3 were detected using an immunohistochemical method. The results indicated that the offspring had significantly impaired SLM, with decreased H3S10p and increased H3K9me3 levels from 12 months onward. Maternal LPS exposure during late gestation significantly and dose-dependently exacerbated the age-related impairment of SLM, with the decrease in H3S10p and increase in H3K9me3 beginning at 12 months in the offspring. The histone modifications (H3S10p and H3K9me3) were significantly correlated with impairment of SLM. Our findings suggest that prenatal exposure to inflammation could exacerbate age-related impairments of SLM and changes in histone modifications in CD-1 mice from 12 months onward, and SLM impairment might be linked to decreased H3S10p and increased H3K9me3.

5.
Chem Biol Interact ; 292: 24-29, 2018 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-29932878

RESUMO

Elevated cyclooxygenase-2 (COX-2) closely associates with tumor progression and distant metastasis in various human cancers. However, the role of COX-2 in epithelial ovarian cancer (EOC), and its mechanistic details, remain poorly understood. In the present study, we tested hypothesis that COX-2 induces loss of expression of E-cadherin, with resulting promotion of cancer cells' invasiveness in ovarian cancer. First, we observed an inverse relationship between COX-2 and E-cadherin expression as COX-2 was enhanced but E-cadherin was decreased in surgically-resected specimens of EOC. Depletion of COX-2, by celecoxib treatment, resulted in attenuated nuclear translocation of Snail, and, in turn, significantly increased E-cadherin in EOC cell line SKOV3, which was established to be due to the reduced binding of Snail onto E-cadherin promoter. Such COX-2 inhibition resulted in reduced invasion of EOC cells, similar to what was achieved through Snail silencing in SKOV as well as ES-2 EOC cells. These results suggest that COX-2-Snail signaling plays a critical role in regulation of E-cadherin and might provide insights into mechanisms for paracrine inflammation-mediated aggressiveness in EOC.


Assuntos
Caderinas/genética , Caderinas/metabolismo , Celecoxib/farmacologia , Ciclo-Oxigenase 2/metabolismo , Neoplasias Epiteliais e Glandulares/fisiopatologia , Neoplasias Ovarianas/fisiopatologia , Fatores de Transcrição da Família Snail/metabolismo , Western Blotting , Caderinas/antagonistas & inibidores , Carcinoma Epitelial do Ovário , Linhagem Celular Tumoral , Regulação para Baixo , Feminino , Humanos , Inflamação/tratamento farmacológico , Neoplasias Epiteliais e Glandulares/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , Transdução de Sinais/efeitos dos fármacos
6.
Yi Chuan ; 40(3): 197-206, 2018 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-29576543

RESUMO

The semen quality is a basic and the most important indicator of male reproductive health. Mammalian spermatozoa undergo a series of complex structural and functional changes in the epididymis to mature and achieve fertilization capacity. Sperm cell maturation is mediated by a complex physiological process, which is synergistically regulated by a large number of transcription factors, hormones and other signaling molecules. In recent years, there is increasing evidence supporting the notion that functional proteins and sRNA (small RNAs) in epididymosomes participate in sperm maturation and fertilization process. In this review, we summarize the biological roles of functional proteins and two major sRNAs (tRNAs and miRNAs) in sperm maturation in epididymosomes, and provide some theoretical guidance and new ideas for treatments of low fertility, infertility and other reproductive diseases in men.


Assuntos
Epididimo/metabolismo , MicroRNAs/metabolismo , RNA de Transferência/metabolismo , Espermatozoides/metabolismo , Animais , Humanos , Masculino , MicroRNAs/genética , RNA de Transferência/genética , Maturação do Esperma , Espermatozoides/citologia
7.
Age (Dordr) ; 38(5-6): 405-418, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27631330

RESUMO

Age-related cognitive decline has been associated with changes in endogenous hormones and epigenetic modification of chromatin, including histone acetylation. Developmental exposure to endocrine disrupting chemicals, such as bisphenol-A (BPA) that produces endocrine disruption and epigenetic changes, may be a risk factor for accelerating cognitive deficits during aging. Thus, we exposed CD-1 mice to BPA (0, 1, and 100 mg/l BPA in the drinking water) orally during puberty (from postnatal days 28 to 56) and investigated whether pubertal BPA exposure exacerbates the age-related impairment of spatial cognition in old age (18 months old) and whether serum sex and thyroid hormones or hippocampal histone acetylation (H3K9ac and H4K8ac) are associated with cognitive effects. A young control group (6 months old) was added to analyze the age effect. Results showed untreated aged mice had marked decline of spatial learning and memory in the novel location recognition and radial six-arm water maze tasks, with decreased levels of these hormones and hippocampal H3K9ac and H4K8ac compared to young controls. The BPA treatment exacerbated age-related spatial cognitive impairment and accelerated the reduction of free thyroxine (FT4), H3K9ac, and H4K8ac, and the 100 mg/l BPA group showed more significant impact. Additionally, correlation analyses revealed that lower levels of FT4, H3K9ac, and H4K8ac were accompanied by decreased spatial memory abilities. We concluded that accelerated reduction of serum FT4 and hippocampal H3K9ac and H4K8ac might be linked to exacerbation of age-related spatial cognitive impairment due to pubertal BPA exposure.


Assuntos
Envelhecimento/efeitos dos fármacos , Compostos Benzidrílicos/toxicidade , Disruptores Endócrinos/toxicidade , Hipocampo/efeitos dos fármacos , Transtornos da Memória/induzido quimicamente , Fenóis/toxicidade , Memória Espacial/efeitos dos fármacos , Tiroxina/sangue , Acetilação , Animais , Escala de Avaliação Comportamental , Disfunção Cognitiva/sangue , Disfunção Cognitiva/induzido quimicamente , Disfunção Cognitiva/metabolismo , Progressão da Doença , Feminino , Hipocampo/metabolismo , Histonas/metabolismo , Masculino , Aprendizagem em Labirinto , Transtornos da Memória/sangue , Transtornos da Memória/metabolismo , Camundongos , Fatores de Risco , Fatores de Tempo
8.
Psychophysiology ; 53(10): 1567-76, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27412857

RESUMO

Memory impairment is a frequent complaint in insomniacs; however, it is not consistently demonstrated. It is unknown whether memory impairment in insomniacs involves neuroendocrine dysfunction. The participants in this study were selected from the clinical setting and included 21 patients with chronic insomnia disorder (CID), 25 patients with insomnia and comorbid depressive disorder (CDD), and 20 control participants without insomnia. We evaluated spatial working and reference memory, object working and reference memory, and object recognition memory using the Nine Box Maze Test. We also evaluated serum neuroendocrine hormone levels. Compared to the controls, the CID patients made significantly more errors in spatial working and object recognition memory (p < .05), whereas the CDD patients performed poorly in all the assessed memory types (p < .05). In addition, the CID patients had higher levels (mean difference [95% CI]) of corticotrophin-releasing hormone, cortisol (31.98 [23.97, 39.98] µg/l), total triiodothyronine (667.58 [505.71, 829.45] µg/l), and total thyroxine (41.49 [33.23, 49.74] µg/l) (p < .05), and lower levels of thyrotropin-releasing hormone (-35.93 [-38.83, -33.02] ng/l), gonadotropin-releasing hormone (-4.50 [-5.02, -3.98] ng/l) (p < .05), and adrenocorticotropic hormone compared to the CDD patients. After controlling for confounding variables, the partial correlation analysis revealed that the levels of cortisol positively correlated with the errors in object working memory (r = .534, p = .033) and negatively correlated with the errors in object recognition memory (r = -.659, p = .006) in the CID patients. The results suggest that the CID patients had selective memory impairment, which may be mediated by increased cortisol levels.


Assuntos
Hidrocortisona/sangue , Memória , Distúrbios do Início e da Manutenção do Sono/sangue , Distúrbios do Início e da Manutenção do Sono/psicologia , Adulto , Transtorno Depressivo/sangue , Transtorno Depressivo/complicações , Transtorno Depressivo/psicologia , Feminino , Humanos , Masculino , Memória de Curto Prazo , Pessoa de Meia-Idade , Distúrbios do Início e da Manutenção do Sono/complicações , Memória Espacial , Adulto Jovem
9.
Age (Dordr) ; 38(3): 59, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27194408

RESUMO

Data shows that inflammation during pregnancy significantly exerts a long-term influence on offspring, such as increasing the risk of adult cognition decline in animals. However, it is unclear whether gestational inflammation affects the neurobehavioral and neurobiochemical outcomes in the mother-self during aging. In this study, pregnant CD-1 mice intraperitoneally received lipopolysaccharide (LPS) in two doses (25 and 50 g/kg, respectively) or normal saline daily during gestational days 15-17. At the age of 15 months, a battery of behavioral tasks was employed to evaluate their species-typical behaviors, sensorimotor ability, anxiety levels, and spatial learning and memory abilities. An immunohistochemical method was utilized preliminarily to detect neurobiochemical indicators consisting of amyloid-ß, phosphorylated tau, presynaptic proteins synaptotagmin-1 and syntaxin-1, glial fibrillary acidic protein (GFAP), and histone-4 acetylation on the K8 site (H4K8ac). The behavioral results showed that LPS exposure during pregnancy exacerbated a decline in 15-month-old CD-1 mice's abilities to nest, their sensorimotor and spatial learning and memory capabilities, and increased their anxiety levels. The neurobiochemical results indicated that gestational LPS exposure also intensified age-related hippocampal changes, including increased amyloid-ß42, phosphorylated tau, synaptotagmin-1 and GFAP, and decreased syntaxin-1 and H4K8ac. Our results suggested that the inflammatory insult during pregnancy could be an important risk factor for the development of Alzheimer's disease, and the H4K8 acetylation might play an important role in the underlying mechanism. This study offers a perspective for improving strategies that support healthy development and successful aging.


Assuntos
Envelhecimento/metabolismo , Comportamento Animal , Transtornos Cognitivos/etiologia , Inflamação/complicações , Complicações Infecciosas na Gravidez , Prenhez , Acidente Vascular Cerebral/complicações , Animais , Biomarcadores/metabolismo , Transtornos Cognitivos/metabolismo , Transtornos Cognitivos/psicologia , Modelos Animais de Doenças , Feminino , Imuno-Histoquímica , Inflamação/metabolismo , Masculino , Memória/fisiologia , Camundongos , Gravidez , Acidente Vascular Cerebral/metabolismo , Acidente Vascular Cerebral/psicologia
10.
Behav Brain Res ; 306: 178-96, 2016 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-26992827

RESUMO

Maternal inflammation during pregnancy can elevate the risk of neurodegenerative disorders in offspring. However, how it affects age-related impairments of spatial learning and memory and changes in the neurobiological indictors in the offspring in later adulthood is still elusive. In this study, the CD-1 mice with maternal gestational inflammation due to receiving lipopolysaccharide (LPS, i.p. 50 or 25µg/kg) were divided into 3-, 12-, 18-, and 22-month-old groups. The spatial learning and memory were evaluated using a six-radial arm water maze and the levels of presynaptic proteins (synaptotagmin-1 and syntaxin-1) and histone acetylation (H3K9ac and H4K8ac) in the dorsal hippocampus were detected using the immunohistochemical method. The results indicated that there were significant age-related impairments of spatial learning and memory, decreased levels of H4K8ac, H3K9ac, and syntaxin-1, and increased levels of synaptotagmin-1 in the offspring mice from 12 months old to 22 months old compared to the same-age controls. Maternal LPS treatment significantly exacerbated the offspring impairments of spatial learning and memory, the reduction of H3K9ac, H4K8ac, and syntaxin-1, and the increment of synaptotagmin-1 from 12 months old to 22 months old compared to the same-age control groups. The changes in the neurobiological indicators significantly correlated with the impairments of spatial learning and memory. Furthermore, this correlation, besides the age and LPS-treatment effects, also showed a dose-dependent effect. Our results suggest that maternal inflammation during pregnancy could exacerbate age-related impairments of spatial learning and memory, and neurobiochemical indicators in the offspring CD-1 mice from midlife to senectitude.


Assuntos
Envelhecimento , Inflamação/complicações , Transtornos da Memória/etiologia , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Aprendizagem Espacial/fisiologia , Envelhecimento/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Feminino , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Histonas/metabolismo , Inflamação/etiologia , Lipopolissacarídeos/toxicidade , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/fisiologia , Aprendizagem Espacial/efeitos dos fármacos , Sinaptotagmina I/metabolismo , Sintaxina 1/metabolismo
11.
Age (Dordr) ; 37(5): 102, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26400487

RESUMO

Several studies have indicated that a caloric restriction mimetic or treatment for type 2 diabetes may reverse brain aging. Therefore, we investigated the effect of 1-deoxynojirimycin (DNJ), an alkaloid acting as an inhibitor of α-glucosidase, on age-related behavioral and biochemical changes. SAMP8 mice were randomly assigned to a control group labeled "old" or to the 10- or 20-mg/kg/day DNJ groups. The mice in the DNJ groups were administered DNJ orally from 3 to 9 months of age, and then, a "young" control group was added to analyze the age effect. The old controls exhibited significant declines in sensorimotor ability, open-field anxiety, spatial and nonspatial memory abilities, and age-related biochemical changes, including decreased serum insulin level; increased levels of insulin-like growth factor 1 receptor, presynaptic protein synaptotagmin-1, and astrocyte activation; and decreased levels of insulin receptor, brain-derived neurotrophic factor, presynaptic protein syntaxin-1, and acetylation of histones H4 at lysine 8 in the dorsal hippocampus. Significant correlations exist between the age-related behavioral deficits and the serological and histochemical data. Chronic DNJ treatment alleviated these age-related changes, and the 20-mg/kg/day DNJ group showed more significant improvement. Thus, DNJ may have the potential to maintain successful brain aging.


Assuntos
1-Desoxinojirimicina/administração & dosagem , Envelhecimento/efeitos dos fármacos , Comportamento Animal/fisiologia , Encéfalo/metabolismo , Cognição/efeitos dos fármacos , Aprendizagem em Labirinto/efeitos dos fármacos , Administração Oral , Animais , Encéfalo/efeitos dos fármacos , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/administração & dosagem , Feminino , Masculino , Camundongos
12.
Behav Brain Res ; 284: 138-52, 2015 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-25698601

RESUMO

The administration of maintaining the homeostasis of insulin/insulin-like growth factor 1 (IGF-1) signaling and/or glucose metabolism may reverse brain aging. In the present study, we investigated the effect of acarbose, an inhibitor of α-glucosidase, on age-related behavioral and biochemical changes. The SAMP8 mice were randomly divided into old control group and acarbose-treatment group. The mice in the acarbose group were administered acarbose (20 mg/kg/d, dissolved in drinking water) orally from 3 to 9 months of age when a new group of 3-month-old mice was added as young controls. The results showed that the aged controls exhibited declines in sensorimotor ability, open field anxiety, spatial and non-spatial memory abilities, decreased serum insulin levels, increased IGF-1 receptor and synaptotagmin 1 (Syt1) levels and decreased insulin receptor, brain-derived neurotrophic factor (BDNF) and syntaxin 1 (Stx1) levels in the hippocampal layers. The age-related behavioral deficits correlated with the serological and histochemical data. Chronic acarbose treatment relieved these age-related changes, especially with respect to learning and memory abilities. This protective effect of acarbose on age-related behavioral impairments might be related to changes in the insulin system and the levels of BDNF, IGF-1R, and the pre-synaptic proteins Syt1 and Stx1. In conclusion, long-term treatment with acarbose ameliorated the behavioral deficits and biochemical changes in old SAMP8 mice and promoted successful aging. This study provides insight into the potential of acarbose for the treatment of brain aging.


Assuntos
Acarbose/farmacologia , Envelhecimento/efeitos dos fármacos , Envelhecimento/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Psicotrópicos/farmacologia , Administração Oral , Envelhecimento/patologia , Envelhecimento/psicologia , Animais , Fator Neurotrófico Derivado do Encéfalo/sangue , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Água Potável , Feminino , Hipocampo/efeitos dos fármacos , Hipocampo/patologia , Hipocampo/fisiologia , Insulina/sangue , Masculino , Memória/efeitos dos fármacos , Memória/fisiologia , Camundongos , Atividade Motora/efeitos dos fármacos , Atividade Motora/fisiologia , Distribuição Aleatória , Receptor IGF Tipo 1/metabolismo , Receptor de Insulina/metabolismo , Sinaptotagmina I/metabolismo , Sintaxina 1/metabolismo
13.
Yi Chuan ; 35(7): 830-8, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-23853353

RESUMO

Lipoprotein lipase (LPL) is an essential enzyme in the lipid metabolism, and proper regulation of LPL is important for controlling the delivery of lipid nutrients to tissues. Recent studies have identified glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1(GPIHBP1) as the important regulation factor of LPL that serves as a binding platform for lipolysis on the vascular lumen and an endothelial cell transporter transporting LPL from the interstitial spaces to the capillary lumen. In addition, several other regulation factors of LPL have also been identified including microRNAs, SorLA (Sortilin-related receptor with A-type repeats), and apolipoproteins that are potentially important for regulating LPL activity. These discoveries provide new directions for understanding basic mechanisms of lipolysis and hyperlipidemia. In this update, we focused on summarizing recent progresses on GPIHBP1, the endothelial cell LPL transporter. We also highlighted the recent progresses on several other regulation factors of LPL that are relevant to the regulation of LPLactivity.


Assuntos
Regulação da Expressão Gênica , Lipase Lipoproteica/genética , Lipase Lipoproteica/metabolismo , Animais , Humanos , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Receptores de Lipoproteínas/genética , Receptores de Lipoproteínas/metabolismo
14.
Yi Chuan ; 35(7): 890-5, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-23853360

RESUMO

The purpose of this work was to elucidate the characteristics and the expression profiles of porcine Opn4 during developmental stages and different times of the day. The coding sequence of Opn4 gene was cloned. The mRNA expression levels of Opn4 gene in the retina of the swines during developmental stages and different times of the day were examined. The results showed that the swine ORF of Opn4 gene was 1 437 bp in length which encodes 478 amino acids residues with the molecular formula of C2398H3705N623O651S23. The expression level of Opn4 in the day was significantly higher than that in the night (P<0.01). During the growing periods, Opn4 started expressing as early as 34 d in the embryonic phase with the lowest level (P<0.01), reached to a peak 1 day after birth (P<0.01), and then stayed at a moderate standard. The difference of Opn4 expressions among the swines at 1th month, 4th month, and 84th month was not significantly different (P>0.05). In total, results showed that the Opn4 gene can regulate the biological rhythm of the swines. Furthermore, it play an important role in mediate the responsibility to the light in the postnatal swines.


Assuntos
Clonagem Molecular , Regulação da Expressão Gênica no Desenvolvimento , Opsinas de Bastonetes/genética , Suínos/genética , Animais , DNA Complementar/genética , DNA Complementar/metabolismo , Feminino , Humanos , Masculino , Dados de Sequência Molecular , Retina , Opsinas de Bastonetes/metabolismo , Suínos/crescimento & desenvolvimento , Suínos/metabolismo
15.
Yi Chuan ; 35(9): 1081-6, 2013 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-24400481

RESUMO

Satellite cell is a kind of myogenic stem cells, which plays an important role in muscle development and injury repair. Through proliferation, differentiation and fusion of muscle fiber can satellite cells make new myonuclear, leading to the hypertrophy of skeletal muscle and fiber type transformation, and this would further affect the meat quality. Here, we review the relationship between muscle fiber development and meat quality attributes as well as the influence of the satellite cell differentiation on muscle fiber character. Besides, we also summarize the classical signaling pathway (i.e., Notch etc.) and influence of epigenetic regulation (i.e. miRNA) on muscle quality.


Assuntos
Carne/análise , Músculo Esquelético/crescimento & desenvolvimento , Células Satélites de Músculo Esquelético/metabolismo , Animais , Epigênese Genética , Músculo Esquelético/química , Músculo Esquelético/metabolismo , Controle de Qualidade , Células Satélites de Músculo Esquelético/química , Células Satélites de Músculo Esquelético/citologia , Transdução de Sinais
16.
J Tradit Chin Med ; 32(1): 125-8, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22594116

RESUMO

OBJECTIVE: To analyze the cost effect of surrounding acupuncture plus electric acupuncture, cotton-sheet moxibustion, puncturing with red-hot needles, tapping plus cupping on herpes zoster. METHODS: Five hundred patients with herpes zoster were randomly divided into group A (surrounding acupuncture plus electric acupuncture), group B (cotton-sheet moxibustion), group C (puncturing with red-hot needles), group D (tapping plus cupping), and group E (Western medicine). The treatment was carried out twice a day in group E and once a day in the other four groups. The curative effect was observed on the 10th day of treatment; the cost was calculated for the five therapies, and the cost-effect ratio (C/E) and increment ratio (delta C/delta E) were analyzed. RESULTS: After the 10-day treatment, there was no statistical difference (P > 0.05) in the curative effect among the five groups. Pain being alleviated one day faster than in group E amounted to a saving of RMB 21.90 yuan in group A, a saving of RMB 21.87 yuan in group B, a saving of RMB 26.00 yuan in group C, and a saving of RMB 20.23 yuan in group D. Compared with group C, the values of delta C/delta E were RMB 1.55, 2.81, and 0.21 yuan in groups A, B, and D, respectively. CONCLUSIONS: The curative effect in groups A, B, C, and D was similar to that in group E, but the C/E was better than in group E.


Assuntos
Terapia por Acupuntura/economia , Herpes Zoster/terapia , Moxibustão/economia , Manejo da Dor/economia , Terapia por Acupuntura/métodos , Adulto , Idoso , Feminino , Herpes Zoster/economia , Humanos , Masculino , Pessoa de Meia-Idade , Moxibustão/métodos , Manejo da Dor/métodos
17.
In Vitro Cell Dev Biol Anim ; 48(1): 54-60, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22170349

RESUMO

The procedure of preadipocyte differentiation to mature adipocytes is controlled by various transcription factors; these factors activate and regulate the fat formation genes through a series of complex steps. To investigate the time line of gene expression of several potential genes and make a observation, we isolated preadipocytes from subcutaneous adipose tissue of 2-d old piglets by collagenase digestion approach and extracted total RNA from the cells, then measured mRNA expression level of AdipoR1, IGFBP3, PPARγ, PPARGC1, FASN, FABP4, and C/EBPα at 10 different time points via real-time quantitative RT-PCR method. The results revealed that the expression of AdipoR1 and IGFBP3 was both upregulated to the maximum at 8 h, the expression of PPARγ, PPARGC1, FASN, FABP4, and C/EBPα was all upregulated to the maximum at 9 d, and these genes were in significant correlation. We present tentatively conclusions that, the gene expression of AdipoR1 and IGFBP3 is upregulated in the early stage of preadipocyte differentiation, and the gene PPARγ, PPARGC1, FASN, FABP4, and C/EBPα reached a high expression in the later period. The expression variation tendency of these genes suggests that they may influence on each other in a sort of way. However, the specific mechanism that AdipoR1, IGFBP3, PPARγ, and the related genes how to cooperate or interact with each other still remains to be further explored.


Assuntos
Adipócitos/metabolismo , Tecido Adiposo/crescimento & desenvolvimento , Diferenciação Celular/genética , Regulação da Expressão Gênica no Desenvolvimento , Adipócitos/citologia , Tecido Adiposo/citologia , Animais , Suínos
18.
Interdiscip Sci ; 2(2): 175-9, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20640787

RESUMO

Ten different pig breeds with different genetic origin (they are eight Chinese Domestic breeds, one recently Developed breed and one Introduced breed respectively) were utilized in this study to analyze the phylogeny of MyoG gene. 63 individuals were sequenced to get their complete nucleotide sequences of MyoG gene. Different methods and software were used and 6 phylogenetic trees were got based on the complete nucleotide sequence. To test the validation of these phylogenetic trees, the topologic structure differences between different trees were compared. The results indicate that the Neighbour Joining tree of the MyoG gene (Figure 2, NJ tree) has the best topological structure. In this NJ tree, 6 Chinese Domestic breeds cluster in one branch, Dahe and Wuzhishan pig constructed a new branch, Dahewu pig, Duroc and Standard MyoG gene sequence (Genbank accession number X89007) are clustered in one branch, the results are concord with the breeding history of these breeds and can reflect their true genetic background. Same topologic structure and parameters were found between different phylogenetic trees got by different methods. The same parameters of these trees confirmed the fact that the MyoG gene is very conservative and there are very few mutation sites between different breeds.


Assuntos
Miogenina/genética , Miogenina/metabolismo , Animais , China , Biologia Computacional/métodos , Frequência do Gene , Modelos Genéticos , Mutação , Oligonucleotídeos/genética , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Software , Especificidade da Espécie , Suínos
19.
Zhongguo Zhen Jiu ; 30(3): 218-20, 2010 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-20496738

RESUMO

In clinical researches, the authors find that cotton-pave moxibustion has obvious effect for treatment of common dermatopathy, such as herpes zoster. This therapy has some advantages such as easy operation, obvious clinical effect, slight and short burning pain and easy to be accepted by patients. The purpose of this paper is to make the operation of cotton-pave moxibustion standardized, spread and popularize the application of this therapy in dermatopathy through detailed description to the operation method and points for attention of cotton-pave moxibustion and its typical cases.


Assuntos
Moxibustão/métodos , Dermatopatias/terapia , Humanos , Masculino , Pessoa de Meia-Idade
20.
Yi Chuan ; 31(8): 837-43, 2009 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-19689945

RESUMO

Real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of insulin like growth factor-1 and -2 (IGF-1 and -2), insulin like growth factor receptor -1 and -2 (IGF-1R and -2R), insulin like growth factor binding protein-3 and -5 (IGFBP-3 and -5) genes at the age of birth (0), 1, 2, 3, 4, and 5 months in longissimus dorsi muscle of Landrace and Meishan pigs. The expression level of IGF-1 exhibited up-regulation, in contrast, the expression level of IGF-2 showed down-regulation after birth in the two pig breeds. These findings are consistent with the previous knowledge that IGF-2 is mainly expressed during the embryonic phase, IGF-1 is mainly expressed during the postnatal period, which could promote cell proliferation and ontogenesis. The expression patterns of IGFRs and IGFs were dissimilar from 0 to 5 months in the two pig breeds, which suggest that the expression changes of IGFRs may not be subjected to the regulation by local secretion of IGFs. Strikingly, at the age of 2 months, the expression levels of IGF-1R, IGF-2R, and IGFBP-3 reached their maximum peak values in Landrace pigs, which suggest that the 2 months after birth may be the most important growth stage for genes of insulin-like growth factors (IGFs) system to exert their biological functions from birth to 5 months. These results indicate that the mRNAs of IGFs system genes exhibit specific developmental expression changes between Landrace and Meishan pigs, and provides some data for further study on the mutual regulation mechanism of genes in IGFs system.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/genética , Músculo Esquelético/crescimento & desenvolvimento , Receptores de Somatomedina/genética , Suínos/crescimento & desenvolvimento , Suínos/genética , Animais , Feminino , Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/metabolismo , Masculino , Músculo Esquelético/metabolismo , Receptores de Somatomedina/metabolismo , Somatomedinas/genética , Somatomedinas/metabolismo , Suínos/metabolismo
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