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1.
Artigo em Chinês | MEDLINE | ID: mdl-32062902

RESUMO

Pulmonary fibrosis is the terminal manifestation of a variety of interstitial lung diseases. Idiopathic pulmonary fibrosis (IPF) is one of the chronic, progressive, fibrotic lung disease with high incidence and poor prognosis. Nintedanib and pirfenidone are currently marketed anti-pulmonary fibrosis drugs, and their efficacy and safety are recognized in patients with IPF. This article reviews the targets and clinical trials of the two drugs, and provides a basis for the expansion of indications for anti-pulmonary fibrosis drugs.

2.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(1): 2-5, 2020 Jan 06.
Artigo em Chinês | MEDLINE | ID: mdl-31914560

RESUMO

In the past decade, the incidence and mortality of malignant tumors in China have been growing as a serious public health problem. Health literacy is closely related to the individual cancer risk awareness, early cancer symptom recognition, cancer screening behavior, treatment compliance, disease self-management ability and outcome. It also has an important impact on the occurrence, development and outcome of cancer and plays an important role in the tertiary cancer prevention. This issue focuses on the consciousness of cancer prevention, early detection, early diagnosis and early treatment, as well as the demand of and access to the cancer prevention and treatment knowledge, and could provide reference for cancer prevention and control in urban residents.


Assuntos
Alfabetização em Saúde , Neoplasias/prevenção & controle , China , Conhecimentos, Atitudes e Prática em Saúde , Humanos
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(1): 108-112, 2020 Jan 06.
Artigo em Chinês | MEDLINE | ID: mdl-31914578

RESUMO

The health literacy refers to the ability of individuals to acquire and understand basic health information and services and use them to make the right decisions to maintain and promote their health. Health literacy data focusing on cancer prevention and control was limited in China. In order to understand the health literacy and awareness of cancer risk factors and the cancer screening, early diagnosis and treatment in Chinese urban residents and compare the effect of different stages of the cancer intervention, the Cancer Screening Program in Urban China (CanSPUC) program, supported by the National Key Public Health Program, conducted a survey on the health literacy of the cancer prevention and treatment among urban residents in 16 provinces nationwide from 2015 to 2017. Four subgroups were designed in this survey, including (1) general population who have never participated in any cancer screening programs at a community-level; (2) individuals who have previously attended the CanSPUC program for cancer risk assessment or screening intervention; (3) cancer patients who were receiving treatment in local hospitals; (4) a special group from employees of government and public institutions (non-health system), state-owned enterprises and private enterprises (to have better understand on the impact of socioeconomic factors). The self-designed questionnaire covered six parts, including basic information, consciousness of common risk factors to cancer, awareness of early detection, awareness of early diagnosis, awareness of early treatment, and the needs and approaches for knowledge of cancer prevention and treatment. A total of 32 257 individuals were included in the final analyses. This paper landscaped the overall design of the survey, including participants, domains of the instrument, quality control, basic characteristics of the included individuals. These descriptions are applicable to each individual report of the current special issue of "Health Literacy of Cancer Control in Urban China" and future reports, in which more detailed results are and will be reported. The findings of this survey could provide some useful implications for similar researches in the future.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Alfabetização em Saúde , Neoplasias/prevenção & controle , População Urbana , China , Humanos , Fatores de Risco , Inquéritos e Questionários
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 54(1): 113-116, 2020 Jan 06.
Artigo em Chinês | MEDLINE | ID: mdl-31914579

RESUMO

This article reviews the literature on health literacy and cancer prevention, screening, treatment and prognosis. A large number of studies have shown that health literacy is positively correlated with public cancer risk awareness, cancer knowledge awareness rate, and cancer screening behavior, and positively correlated with the health service resource utilization ability of cancer patients, treatment compliance, and quality of life, and negatively correlated with the unhealthy lifestyle such as smoking and drinking. Some studies have problems such as small sample size, limited population, and inappropriate design. Some studies do not support the conclusions above. Therefore, multi-center, large-scale clinical studies and cohort studies should be conducted to provide more robust evidence for the relationship between the health literacy and cancer prevention, screening, and treatment.


Assuntos
Alfabetização em Saúde , Neoplasias/prevenção & controle , Humanos
5.
J Phys Condens Matter ; 32(1): 015303, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31499486

RESUMO

The monolayer InSe has been successfully fabricated recently and studied intensely. Here, we investigate the geometrical stability and various physical properties such as electronic and magnetic feature, carrier mobility and strain effects for InSe nanoribbons. Our calculations show that armchair nanoribbons, regardless of the bare-edged or H-saturated ones, are semiconductors with an indirect bandgaps, but the bandgap size is increased greatly by H-saturation. Their electron mobility is predicted to be moderately large (from ~102 to ~103 cm2 V-1 s-1) with the holes being less mobile for wider ribbons, and the carrier polarity phenomenon becomes more prominently for H-saturation. The zigzag InSe nanoribbons are found to be magnetic metals with a bigger magnetic moment and the ferromagnetic ground state at the single edge. The magnetism stems from unpaired electrons at the In-rich edge. More interestingly, it is found that the externally applied mechanical strain can effectively tune the spin polarization efficiency at the Fermi level to two stepwise stages, suggesting that the strain can act as a tool for developing a mechanical switch to control spin-polarized transport under lower bias. The detailed analysis suggests that this strain-tuning mechanism can be attributed to the ionic and covalent bond-configuration competition due to the strain-induced bond-length alterations, which leads to the unpaired electron redistribution in magnetic atoms or vanishing.

6.
J Appl Microbiol ; 128(1): 255-264, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31541488

RESUMO

AIMS: To conduct molecular tagging of the biocontrol fungus Trichoderma asperellum strain T4 and elucidate its colonization patterns in soil. METHODS AND RESULTS: We constructed an expression vector harbouring a hygromycin B-resistant gene (hph) and an efficient green fluorescent protein (egfp) gene. By applying Agrobacterium AGL-1-mediated genetic transformation technology, we conducted molecular tagging of T. asperellum and monitored the colonization dynamics of T. asperellum in soil. The results of tracking five independent transformants of T. asperellum indicated that its expansion rates ranged from 4·7 to 6·8 cm week-1 . After inoculation in soil, the quantities of T. asperellum could be maintained at over 10 × 104  CFU per gram soil in the first year. In the third year after inoculation, the quantities of T. asperellum in soil were still higher than 1 × 103  CFU per gram soil. In addition, molecularly tagged T. asperellum in soil in the second year (i.e. 12 months) after inoculation could still reach the biocontrol effect on cucumber Rhizoctonia rot by more than 74%. CONCLUSION: Trichoderma asperellum strain T4 is capable of effectively colonizing in soil and surviving for more than 1 year. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has provided the scientific basis for applying T. asperellum as the biocontrol fungus for prevention and control of plant diseases.


Assuntos
Agentes de Controle Biológico , Microbiologia do Solo , Trichoderma/crescimento & desenvolvimento , Trichoderma/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Agentes de Controle Biológico/farmacologia , Contagem de Colônia Microbiana , Cucumis sativus/microbiologia , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Rhizoctonia/efeitos dos fármacos , Trichoderma/metabolismo
7.
Zhonghua Yi Xue Za Zhi ; 99(48): 3786-3791, 2019 Dec 24.
Artigo em Chinês | MEDLINE | ID: mdl-31874515

RESUMO

Objective: To evaluate the clinical outcomes in patients with relapsed or refractory peripheral T-cell lymphoma (PTCL) who had undergone allogeneic hematological stem cell transplantation (allo-HSCT). Methods: From June 2007 to June 2017, the clinical data of PTCL patients who underwent HSCT from eight hospitals were assessed retrospectively. Results: There were 23 patients diagnosed as relapsed or refractory PTCL with chemoresistance who underwent allo-HSCT. Among these patients, 18 were identified as progressive disease (PD) status and 5 patients as stable disease (SD) status before allo-HSCT. Seventeen patients received allo-HSCT from matched sibling donor (MSD),2 patients from matched unrelated donor and 4 patients from related haplo-identical donor (HD). After a median follow-up of 29 months, 21 patients survived longer than 28 days after allo-HSCT. Hematopoietic reconstitution was achieved in 20 of the 21 patients. The median time of myeloid and platelet engraftment were+13 (9-22) d and+16(10-38) d, respectively. The 100-d treatment-related mortality rate was 13.1%. Acute GVHD occurred in 11(47.8%) patients at a median time of 22(6-82) d after transplantation. Grade Ⅱ~Ⅳ aGVHD occurred in 6 patients. Chronic GVHD occurred in 10 patients at a median of 7.9 (3.5-27) months. After a median follow-up of 29 months, 13 patients died after HSCT. Four of them died of complications associated with allo-HSCT, and other 9 patients died of the primary lymphoma. The 3-years cumulative overall survival (OS) and progress-free survival (PFS) were 43.03% (95%CI: 29.79-69.16) and 39.13% (95%CI: 23.50-65.14), respectively. No significant difference was found in the 3-year PFS between patients with PD status and SD status before allo-HSCT (P=0.133). Conclusion: Allo-HSCT can be a promising treatment for relapsed or refractory PTCL with chemoresistance.


Assuntos
Doença Enxerto-Hospedeiro , Transplante de Células-Tronco Hematopoéticas , Linfoma de Células T Periférico , Resistencia a Medicamentos Antineoplásicos , Humanos , Linfoma de Células T Periférico/tratamento farmacológico , Recidiva Local de Neoplasia , Estudos Retrospectivos
8.
Pol J Vet Sci ; 22(4): 717-723, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31867929

RESUMO

Pseudorabies (PR) outbreaks have devastated many swine farms in several parts of China since late 2011. The outbreak-associated pseudorabies virus (PRV) variant strains exhibited some typical amino acid changes in glycoprotein E (gE), a diagnostic antigen used for discriminating between PRV-infected and vaccinated animals (DIVA). To counteract the potential impact of epitope variations on current serological diagnostics of PRV, we produced monoclonal antibodies (mAbs) against gE protein of one representative PRV variant strain and developed a blocking immunoperoxidase monolayer assay (b-IPMA) for DIVA. The b-IPMA was based on the inhibition of binding between PRV-infected cells and mAb by PRV-specific antibodies present in clinical swine sera and was validated by comparison with a commercial PRV gpI Antibody Test Kit (IDEXX Laboratories, USA). The diagnostic sensitivity, diagnostic specificity and agreement were determined to be 99.25%, 98.18% and 99.02% respectively upon testing 509 serum samples. b-IPMA detected only PRV-specific antibodies and showed no cross- -reactivity with antibodies elicited by gE-deleted vaccine or other common swine pathogens. Thus, b-IPMA has the potential to be used for high-throughput screening of PRV-infected animals in veterinary clinics.

9.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(6): 1014-1018, 2019 Dec 18.
Artigo em Chinês | MEDLINE | ID: mdl-31848496

RESUMO

OBJECTIVE: To investigate and analyse the features of treatment behavior and standardized therapeutic status of patients with psoriatic arthritis (PsA). METHODS: Out patients diagnosed with PsA in People's Hospital of Peking University, Haidian Hospital, People's Hospital of Jianyang City, Central Hospital of Xinxiang City, Integrated Traditional Chinese and Western Medicine Hospital of Cangzhou City, The Third Hospital of Hebei Medical University from February to June 2018 were enrolled in this investigation. The data including gender, age of onset, course of disease, site of first consulting department, time of the first visit and definite diagnosis, follow-up interval, and use of conventional disease modifying anti-rheumatic drugs (cDMARDs) and biological DMARDs (BioDMARDs) were collected and analyzed. RESULTS: In the cross-sectional study, 133 PsA patients were investigated. The mean age of onset was (47±11) years, the male to female ratio was 1.3:1, and mean disease duration was (16±8) years. Rheumatology department was the most common site of first hospital visit (37.6%, 50/133). Orthopedics department and dermatological department were visited by 24.1% (32/133) and 23.3% (31/133), respectively. Ratio of definite diagnosis was the highest in rheumatology department which was 78% (39/50). The ratio of definite diagnosis of dermatological department was the second highest, which was 19.4% (6/31). The mean definite diagnosed time was 7.6 months since the first visit of PsA patients, and diagnosed time was the shortest in rheumatology department, which had statistical significance. 37% PsA patients were treated appropriately in 3 months, 17.3% PsA patients were treated in 3-6 months and 40.2% patients with PsA visited their doctor more than once a year. 48.8% patients hadn't received standardized treatment before visit, and one third patients never received the therapy of DMARDs. Methotrexate was the most commonly used cDMARDs (58.3%), followed by leflunomide (20.5%) and BioDMARDs (19.7%), and biologicals were tumor necrosis factor antagonists. CONCLUSION: In this multi-center study, the first visit department of PsA patients was widely distributed, and most patients were definitely diagnosed in Rheumatology Department. The time of their first visit and definite diagnosis were delayed due to multi factors. Nearly half of the patients did not receive standardized treatment.


Assuntos
Artrite Psoriásica , Adulto , Antirreumáticos , Estudos Transversais , Feminino , Humanos , Masculino , Metotrexato , Pessoa de Meia-Idade , Fatores de Tempo
10.
Zhonghua Zhong Liu Za Zhi ; 41(11): 820-825, 2019 Nov 23.
Artigo em Chinês | MEDLINE | ID: mdl-31770848

RESUMO

Objective: To establish a nude mouse model of subcutaneous lung cancer using dual fluorescence reporting genes of luciferase (Luc) and near-infrared fluorescent protein (iRFP). Methods: The Luc and iRFP expressed lentiviral vector was constructed by Gateway method. After verified by sequencing, the lentivirus particle was prepared and infected into lung cancer A549 cells. Successfully infected A549 (mA549) cells were selected by puromycin and amplified. The expression of Luc and iRFP were observed under fluorescence microscope, and the expression of c-Met protein on the cell surface was detected by immunofluorescence. Twelve female nude mice were randomly divided into 2 groups, 6 in each group. A549 and mA549 cells were inoculated subcutaneously into the right forelimb of nude mice. The growth and fluorescence expression of the tumor were observed by in vivo imaging. The tumor formation was evaluated by hematoxylin-eosin (HE) staining and immunohistochemistry. Results: The Luc and iRFP stably expressed mA549 cell line was successfully constructed. The expressions of iRFP and Luc in mA549 cells were observed under fluorescence microscope. The results of immunofluorescence showed that c-Met protein expressed in both A549 cells and mA549 cells. The growth period of mA549 xenograft in nude mice was moderate and the tumorigenesis rate was 100%. The growth trend of mA549 cells in vivo was not significantly different from that of A549 cells (P>0.05). HE staining and immunohistochemistry results showed that the tumor issues displayed typical histopathological features of tumor. Immunohistochemistry results showed that both A549 and mA549 tumors expressed c-Met protein. Conclusion: A stable, real-time monitoring model of iRFP-Luc-A549 lung cancer cell xenograft in nude mice was successfully constructed.


Assuntos
Neoplasias Pulmonares/patologia , Transplante de Neoplasias/métodos , Células A549 , Animais , Linhagem Celular Tumoral , Feminino , Fluorescência , Genes Reporter , Humanos , Imuno-Histoquímica , Luciferases/genética , Proteínas Luminescentes/genética , Pulmão , Camundongos , Camundongos Nus , Proteínas Proto-Oncogênicas c-met/genética , Distribuição Aleatória
11.
Zhonghua Nei Ke Za Zhi ; 58(12): 911-914, 2019 Dec 01.
Artigo em Chinês | MEDLINE | ID: mdl-31775456

RESUMO

The purpose of this study was to explore the role and mechanism of transient receptor potential M(2) (TRPM(2)) in antigen-induced arthritis (AIA) mice. Twelve C57BL/6 mice and 12 TRPM(2) knockout mice were divided into 4 groups, includingwild type control group, wild type AIA group, TRPM(2) knockout control group and TRPM(2) knockout AIA group, with 6 mice in each group. Methylated bovine serum albumin (mBSA) was used to establish AIA mouse model. The degree of joint swelling and inflammatory cell infiltration were recorded, as well as synovial hyperplasia of the knee joints. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of interleukin (IL)-6, IL-8, chemokine ligand 6 (CXCL-6) and tumor necrosis factor alpha (TNFα) mRNA in synovial cells of knee joints. The results showed that compared with the wild-type AIA group, the TRPM(2) knockout AIA group had more significant synovial proliferation and inflammatory cell infiltration in the synovial tissue.The neutrophil and macrophage counts rather than monocytes in the knee joints of TRPM(2) knockout AIA group were higher than those in wild-type AIA mice. The expression of IL-6, IL-8 and CXCL-6 mRNA were significantly increased in the knock out mice. In summary, TRPM(2) may inhibit inflammatory cytokines such as IL-6 and IL-8 in knee joints of AIA mice by reducing the infiltration of neutrophils and macrophages, the refore alleviates the manifestations of knee arthritis.


Assuntos
Artrite Experimental/imunologia , Artrite Reumatoide/imunologia , Quimiocina CXCL6/imunologia , Interleucina-6/imunologia , Interleucina-8/imunologia , Soroalbumina Bovina/imunologia , Fator de Necrose Tumoral alfa/imunologia , Animais , Antígenos/imunologia , Artrite Reumatoide/genética , Quimiocina CXCL6/genética , Interleucina-6/genética , Interleucina-8/genética , Articulação do Joelho/imunologia , Articulação do Joelho/patologia , Camundongos , Camundongos Endogâmicos C57BL , Reação em Cadeia da Polimerase em Tempo Real , Membrana Sinovial , Fator de Necrose Tumoral alfa/genética
12.
Eur Rev Med Pharmacol Sci ; 23(18): 8011-8017, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31599426

RESUMO

OBJECTIVE: The aim of this study was to investigate the regulatory effect of long non-coding RNA (lncRNA) NKILA on autophagy in sepsis-induced kidney injury. MATERIALS AND METHODS: Sepsis model was successfully established in rats by cecal ligation and puncture (CLP). Hematoxylin and eosin (HE) staining was performed to evaluate the pathological lesions in rat kidney tissues. Subsequently, serum samples of sepsis rats were collected. The levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were determined. Western blot and quantitative real time-polymerase chain reaction (qRT-PCR) were conducted to detect the protein and mRNA expression levels of LC3, Beclin-1, activated caspase-3, p-Akt (308), p-Akt (472), Akt and NKILA in kidney tissues of sepsis rats at different time points, respectively. Subsequently, HK-2 cells were induced with different doses of lipopolysaccharide (LPS) for different time points. The expression levels of the above genes in cells were detected as well. Finally, changes in autophagy and apoptosis in LPS-induced HK-2 cells with the treatment of PI3K pathway inhibitor or Akt inhibitor were observed. RESULTS: Typical pathological lesions were observed in kidney tissues of sepsis rats, with increased serum levels of BUN and SCr. This indicated the successful construction of the sepsis model in rats. The expression levels of LC3, Beclin-1, and NKILA/Akt significantly increased in kidney tissues of sepsis rats. In vitro experiments revealed that NKILA expression in HK-2 cells gradually up-regulated with the increase of LPS dose and prolongation of LPS induction. The expressions of LC3 and NKILA/Akt were higher at 10 mg/L LPS treatment, and 8 h of LPS induction. Furthermore, the treatment of PI3K pathway inhibitor or Akt inhibitor remarkably down-regulated LPS-induced LC3 expression, while it accelerated cell apoptosis and up-regulated NKILA expression. CONCLUSIONS: Autophagy occurs at sepsis-induced kidney injury, which can be regulated by NKILA/Akt pathway.

13.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(10): 1018-1021, 2019 Oct 06.
Artigo em Chinês | MEDLINE | ID: mdl-31607048

RESUMO

Objective: To evaluate the current status and related factors of influenza vaccination among health care workers (HCWs) in tertiary hospitals of Xining city after the implementation of the free influenza vaccination policy. Methods: In August 2018, the cluster sampling method was used to select four medical institutions in Xining that had previously conducted investigations and interventions. All HCWs(excluding logistic staff) in each medical institution were included in the study. A total of 3 260 valid respondents were included. Questionnaires were used to collect the demographic characteristics, influenza and influenza vaccination awareness, implementation of free policy in the influenza epidemic season from 2017 to 2018, influenza vaccination status, awareness of influenza vaccination schedule and free policy. The multivariate logistic regression model was used to analyze related factors of influenza vaccination. Results: The age of respondents was (31.41±5.00) years. The influenza vaccination rate was 6.80% (226/3 260) in 2017-2018 influenza epidemic season. After controlling for related factors, the awareness of the influenza vaccination schedule (OR=17.05, 95%CI: 5.86-49.59), vaccination frequency (OR=8.22, 95%CI: 2.98-22.61) and the free policy (OR=3.15, 95%CI: 1.49-6.67) had higher vaccination rate. Conclusion: The influenza vaccination rate of HCWs in tertiary hospitals of Xining city was low. Increasing the awareness of the vaccination schedule, frequency and free policy may promote the influenza vaccination rate of HCWs.


Assuntos
Pessoal de Saúde , Vacinas contra Influenza , Influenza Humana/epidemiologia , Atitude do Pessoal de Saúde , China , Cidades , Humanos , Estações do Ano , Inquéritos e Questionários , Centros de Atenção Terciária , Vacinação
14.
J Dairy Sci ; 102(10): 8785-8797, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31378493

RESUMO

The quality of milk powder can decrease during storage. In this study, the characteristics of 12 kinds of commercial milk powder from China were investigated. Changes in various indicators were tracked to comprehensively estimate the oxidation stability of different commercial milk powders. The components of the commercial milk powder were different. The percentages of milk fat, protein, and carbohydrates ranged from 9.8 to 28.5 g/100 g, 15.0 to 24.0 g/100 g, and 32.0 to 67.5 g/100 g, respectively. The water activities ranged from 0.2394 to 0.5286. The diameters of the milk fat globules in different commercial milk powder ranged from 13.99 to 41.09 nm. At the same time, the peroxide value of the control sample was low (≤0.14 mEq/kg). After 3 mo of storage, the peroxide values of some of the commercial milk powder increased significantly. The changes in the thiobarbituric acid values during storage did not follow a common trend. The contents of free fat in the different control samples were 0.21 to 1.67 g/100 g, and these values did not increase during storage. After 3 mo of storage, the hydroxymethyl furfural values and b color values of the different commercial milk powder reached their highest levels. The concentrations of typical oxidized flavor compounds in different commercial milk powder increased greatly with prolonged storage time. The level of hexanal was the highest, and the contents in all the samples ranged from 28.56 to 4,071.28 µg/kg after 6 mo of storage and from 5.91 to 6,281.37 µg/kg after 12 mo of storage. Free radicals were found in some of the stored milk powder, and these were shown as single peaks or multiple peaks. The ratios of the peak areas and masses reached 12.42 × 106 to 14.26 × 108. However, the presence of free radicals in the commercial milk powder was not consistent. The water activities and diameters of the fat globules in the commercial milk powder were highly correlated with their oxidation stabilities during storage.


Assuntos
Armazenamento de Alimentos , Leite/química , Animais , China , Cor , Oxirredução , Peróxidos/análise , Pós , Paladar , Água/análise
15.
Zhonghua Wei Chang Wai Ke Za Zhi ; 22(7): 648-655, 2019 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-31302963

RESUMO

Objective: To investigate the value of colonoscopic assessment in "watch and wait" strategy for mid-lower rectal cancer after neoadjuvant chemoradiotherapy (nCRT). Methods: A single-center retrospective case series study was performed. Database of mid-lower rectal cancer patients at Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute from March 2011 to June 2017 was retrieved. Inclusion criteria: (1) nCRT was completed (50.6 Gy/22 f, plus oral capecitabine); (2) radical surgery was performed within 12 weeks after nCRT treatment; (3) clinical response to nCRT was determined as clinical complete response (cCR) or near-cCR. Patients who did not undergo colonoscopy and MRI in our center during initial assessment and follow-up, or whose colonoscopy data were unable to re-evaluated, were excluded. Initial evaluation of nCRT response was carried out between 6 and 16 weeks after nCRT. The results of endoscopy (eCR, near-eCR and non-eCR) and MRI (mCR, near-mCR and non-mCR) were compared to local lesion relapse during follow-up. The consistency of the results of colonoscopy and MRI was evaluated by Kappa test (Kappa value of 0.21 to 0.40 indicates general consistency, 0.41 to 0.60 moderate consistency, and 0.61 to 0.80 high consistency). The non-regrowth disease-free survival (NR-DFS) curves of the eCR group and the near-eCR group were plotted by Kaplan-Meier method and compared by log-rank test. Clinical significance of colonoscopy examination in the following "watch and wait" strategy during follow-up period was analyzed. Results: A total of 32 patients were enrolled in the study, including 21 (65.6%) males and 11 (34.4%) females with a median age of 57 years old. The differentiated type of rectal cancer included 1 (3.1%) case of well-differentiated, 26 (81.2%) of moderately differentiated and 5 (15.6%) of poorly differentiated. Clinical stage of the patients included 9 (28.1%) cases of T2-3N0 and 23 (71.9%) of T2-3N+. Median follow-up period was 48 (18 to 80) months. The local regrowth rate was 34.4% (11/32) and median interval of local regrowth was 10.0 (4 to 37) months. Initial colonoscopy evaluation was carried out at a median time of 9 (5 to 19) weeks after nCRT was completed. According to endoscopic findings, patients were divided into 3 groups, including 15 cases in eCR group, 15 cases in near-eCR group and 2 cases in non-eCR group. According to the appearance of MRI, patients were divided into 3 groups, including 8 cases in mCR group, 21 cases in near-mCR group and 3 cases in non-mCR group. The regrowth rate of eCR group was lower than that of mCR group (1/15 vs. 1/8) without significant difference (P=1.000). The regrowth rate of near-eCR group was higher than that of near-mCR group [9/15 vs. 42.9% (9/21)] without significant difference as well (P=0.500). The consistency between colonoscopy and MRI in response evaluation of cCR or near-cCR after nCRT was unsatisfactory (Kappa=0.341, P=0.011). After initial evaluation, 31 patients underwent watch and wait strategy, and 1 underwent local resection. The 1- and 3-year NR-DFS in the eCR group was both 100%, which was higher than that in the near-eCR group (53.3% and 38.9%, respectively), and the difference was statistically significant (P=0.001). During watch and wait period, 11 cases developed local regrowth by colonoscopy examination and the biopsy result included 4 case of high-grade intraepithelial neoplasia (HIN), 6 cases of adenocarcinoma and 1 case of chronic mucosal inflammation. Meanwhile lateral developmental tumor of ascending colon in 1 case and of sigmoid in a case was found by colonoscopy and confirmed as HIN by postoperative pathology. Besides, 4 cases developed colonic multiple adenoma and all underwent endoscopic resection. Conclusion: Colonoscopy examination plays an important role in both initial assessment and regrowth monitoring during watch and wait strategy after nCRT treatment.


Assuntos
Adenocarcinoma/diagnóstico , Quimiorradioterapia/métodos , Colonoscopia , Recidiva Local de Neoplasia/diagnóstico , Neoplasias Retais/diagnóstico , Conduta Expectante , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/radioterapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Terapia Neoadjuvante , Neoplasias Retais/tratamento farmacológico , Neoplasias Retais/radioterapia , Estudos Retrospectivos
16.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(7): 668-679, 2019 Jul 06.
Artigo em Chinês | MEDLINE | ID: mdl-31288336

RESUMO

Rabies is a zoonotic infectious disease caused by lyssavirus and characterized by central nervous system symptoms. The fatality rate of rabies is almost 100%. About 59 000 cases die of rabies worldwide every year, mainly in Asia and Africa. China is an epidemic country of rabies. Grade II and III exposures are the main types of rabies exposures in China. Standardized post-exposure prophylaxis (PEP) can prevent rabies almost 100%. Human Rabies Vaccine Technical Working Group, National Immunization Advisory Committee and invited experts reached an expert consensus on PEP by referring to the World Health Organization's position paper on rabies vaccine in 2018 and related research progress in recent.


Assuntos
Profilaxia Pós-Exposição , Raiva/prevenção & controle , China/epidemiologia , Consenso , Humanos , Raiva/epidemiologia , Vacinas Antirrábicas/administração & dosagem
18.
Eur Rev Med Pharmacol Sci ; 23(11): 4800-4806, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31210311

RESUMO

OBJECTIVE: To detect the relative expression of long non-coding ribonucleic acid (lncRNA) F-box and leucine-rich repeat protein 19-antisense RNA 1 (FBXL19-AS1) in tissues and cells of non-small-cell lung cancer (NSCLC), and investigate the mechanism of lncRNA FBXL19-AS1 in promoting NSCLC cell proliferation and metastasis by regulating epithelial-mesenchymal transition (EMT) via in vitro experiments. PATIENTS AND METHODS: The relative expression of lncRNA FBXL19-AS1 in NSCLC tissues and cells was detected via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The colony formation assay was performed to study the impact of interference with lncRNA FBXL19-AS1 expression on NSCLC cell proliferation. The flow cytometry was applied to determine the influence of si-FBXL19-AS1 on the cycle distribution of NSCLC cells. After the interference with lncRNA FBXL19-AS1 expression, the transwell assay was utilized to measure the changes in the migratory and invasive abilities of NSCLC cells, while the expression changes in EMT-related molecular markers was detected via Western blotting. RESULTS: The results of qRT-PCR showed that the expression of lncRNA FBXL19-AS1 in NSCLC tissues and cells was up-regulated. According to the results of the colony formation assay, the proliferative capacity of NSCLC cells was decreased after the interference with lncRNA FBXL19-AS1 expression. In flow cytometry, it was indicated that the cell cycle was arrested at the G0/G1 phase in the experimental group compared with that in the control group. The transwell assay results showed that the migratory and invasive abilities of NSCLC cells were weakened after the interference with lncRNA FBXL19-AS1 expression. The results of the Western blotting assay revealed that the expressions of EMT-related molecular markers (E-cadherin, N-cadherin, etc.) were changed. CONCLUSIONS: The expression of lncRNA FBXL19-AS1 in NSCLC tissues and cells is up-regulated, and the highly expressed lncRNA FBXL19-AS1 can promote NSCLC cell proliferation and metastasis by regulating the EMT.

19.
Eur Rev Med Pharmacol Sci ; 23(11): 4996-5003, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31210337

RESUMO

OBJECTIVE: This study aims to investigate the role of Chi3l1 in Acetaminophen (APAP)-induced liver injury. MATERIALS AND METHODS: In vivo model of liver injury was established in mice administrated with APAP (250 mg/kg) or equivalent phosphate-buffered saline (PBS). Mouse liver tissues were collected at 1 h, 3 h, 6 h, 12 h, and 24 h after treatment, respectively. ALT levels and apoptosis were evaluated. Additionally, we established APAP-induced acute liver injury model in wild-type (WT) mice and Chi3l1-deficient (Chi3l1-/-) mice. Pathological changes of liver tissue were observed by hematoxylin and eosin (HE) staining. Mononuclear cells (MNCs) were isolated from mouse liver, and the amounts of infiltrating macrophages and neutrophils were then counted by flow cytometry. Serum levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Bone marrow-derived macrophages (BMDMs) were extracted from each mouse. RESULTS: After APAP stimulation, Chi3l1-/- mice showed more severe liver injury than that of WT mice, which was manifested as higher ALT levels and more necrotic or apoptotic cells. Compared with WT mice, Chi3l1-/- mice expressed higher levels of inflammatory cytokines (MCP-1 and IL-6), macrophage-associated molecules (CD68 and CD86), as well as the amounts of infiltrating macrophages and neutrophils. In addition, higher expressions of inflammatory cytokines were found in BMDMs extracted from WT mice treated with those BMDM lysates derived from Chi3l1-/- mice than those of non-treated cells. APAP-treated Chi3l1-/- mice exhibited more severe liver injury than that of WT mice. CONCLUSIONS: Our study confirmed that Chi3l1 protects the liver function from APAP-induced injury by inhibiting the secretion of inflammatory factors and macrophage infiltration.

20.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(6): 643-647, 2019 Jun 10.
Artigo em Chinês | MEDLINE | ID: mdl-31238612

RESUMO

Objective: To analyze the epidemiological characteristics, trend and related factors of tuberculosis patients that delayed for care, in Wuhan from 2008 to 2017. Methods: Data regarding tuberculosis (TB) patients was collected from the tuberculosis management information system (TMIS), a part of the China information system for disease control and prevention from 2008 to 2017. A total of 64 208 tuberculosis patients, aged 0 to 95 years were included for the analysis. Unconditional logistic regression method was used to estimate those factors that associated with this study. Results: Days of delay among TB patients appeared as M=10 (P(25)-P(75): 3-28) day, in Wuhan, 2008-2017. The prevalence of the delay was 52.5% (33 703/64 208), presenting a downward trend from 2008 to 2017 (trend χ(2)=10.64, P<0.001), but the proportions of women and ≥65 year-olds were gradually increasing. Results from the multivariate logistic regression analysis showed that factors as: patients living far away from the city vs. near the city (OR=1.29, 95%CI: 1.25-1.35), and age above 45 years vs. younger than 25 years (the age 45-64 years group vs. aged less than 25 years group, OR=1.22, 95%CI: 1.15-1.29; the age 65 or above group vs. aged less than 25 years group, the OR=1.30, 95%CI: 1.22-1.39) were under higher risk on the delay of seeking care. Occupation, way of case-finding and classification of tuberculosis patients also appeared as influencing factors on this issue. Conclusions: Prevalence on the delay of care was 52.5% among tuberculosis patients in Wuhan, 2008-2017, but with an annual decrease. Attention should be paid to female, wrinkly or elderly tuberculosis patients regarding the delay of care on TB, in Wuhan.


Assuntos
Antituberculosos/uso terapêutico , Diagnóstico Tardio , Conhecimentos, Atitudes e Prática em Saúde , Acesso aos Serviços de Saúde , Aceitação pelo Paciente de Cuidados de Saúde/psicologia , Características de Residência/estatística & dados numéricos , Tuberculose Pulmonar/diagnóstico , Tuberculose Pulmonar/tratamento farmacológico , Adolescente , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , China/epidemiologia , Assistência à Saúde/organização & administração , Feminino , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Aceitação pelo Paciente de Cuidados de Saúde/etnologia , Prevalência , Distribuição por Sexo , Tuberculose Pulmonar/epidemiologia , Tuberculose Pulmonar/microbiologia , Adulto Jovem
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