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1.
Artigo em Inglês | MEDLINE | ID: mdl-34285700

RESUMO

Radix Astragali and Semen Lepidii (HQ-TLZ) is a commonly used herbal medicine combination for treatment of heart failure, which has a good clinical effect. However, its active components and mechanism of action are not clear, which limits its clinical application and development. In this study, we explored the mechanism of action of HQ-TLZ in the treatment of heart failure based on network pharmacology. We obtained 11 active ingredients and 109 targets from the TCMSP database and SwissTargetPrediction database. Next, we constructed the action network and carried out enrichment analysis. The results showed that HQ-TLZ treatment of heart failure is primarily achieved by regulating the insulin resistance, erbB signaling pathway, PI3K-Akt signaling pathway, and VEGF signaling pathway. After inverse targeting, molecular docking, and literature search, we determined that the equivalent molecular groups of HQ-TLZ in the treatment of heart failure were quercetin and kaempferol. Based on network pharmacology, we reveal the mechanism of action of HQ-TLZ in the treatment of heart failure to a certain extent. At the same time, we determined the composition of the equivalent molecular group. This provides a bridge for the consistency evaluation of natural herbs and molecular compounds, which is beneficial to the development of novel drugs and further research.

2.
BMC Gastroenterol ; 21(1): 171, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33853536

RESUMO

BACKGROUND: The prevalence of Non-alcoholic fatty liver disease (NAFLD) is increasing and emerging as a global health burden. In addition to environmental factors, numerous studies have shown that genetic factors play an important role in the development of NAFLD. Copy number variation (CNV) as a genetic variation plays an important role in the evaluation of disease susceptibility and genetic differences. The aim of the present study was to assess the contribution of CNV to the evaluation of NAFLD in a Chinese population. METHODS: Genome-wide analysis of CNV was performed using high-density comparative genomic hybridisation microarrays (ACGH). To validate the CNV regions, TaqMan real-time quantitative PCR (qPCR) was utilized. RESULTS: A total of 441 CNVs were identified, including 381 autosomal CNVs and 60 sex chromosome CNVs. By merging overlapping CNVs, a genomic CNV map of NAFLD patients was constructed. A total of 338 autosomal CNVRs were identified, including 275 CNVRs with consistent trends (197 losses and 78 gains) and 63 CNVRs with inconsistent trends. The length of the 338 CNVRs ranged from 5.7 kb to 2.23 Mb, with an average size of 117.44 kb. These CNVRs spanned 39.70 Mb of the genome and accounted for ~ 1.32% of the genome sequence. Through Gene Ontology and genetic pathway analysis, we found evidence that CNVs involving nine genes may be associated with the pathogenesis of NAFLD progression. One of the genes (NLRP4 gene) was selected and verified by quantitative PCR (qPCR) method with large sample size. We found the copy number deletion of NLRP4 was related to the risk of NAFLD. CONCLUSIONS: This study indicate the copy number variation is associated with NAFLD. The copy number deletion of NLRP4 was related to the risk of NAFLD. These results could prove valuable for predicting patients at risk of developing NAFLD.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Biomarcadores , Hibridização Genômica Comparativa , Variações do Número de Cópias de DNA , Genoma , Humanos , Hepatopatia Gordurosa não Alcoólica/genética , Polimorfismo de Nucleotídeo Único
3.
Artigo em Inglês | MEDLINE | ID: mdl-33273955

RESUMO

Fuxin mixture (FXHJ) is a prescription for the treatment of heart failure. It has been shown to be effective in clinical trials, but its active ingredients and mechanism of action are not completely clear, which limits its clinical application and international promotion. In this study, we used network pharmacology to find, conclude, and summarize the mechanism of FXHJ in the treatment of heart failure. From FXHJ, we found 39 active ingredients and 47 action targets. Next, we constructed the action network and was conducted enrichment analysis. The results showed that FXHJ mainly treated heart failure by regulating the MAPK signaling pathway, PI3KAkt signaling pathway, cAMP signaling pathway, TNF signaling pathway, toll-like receptor signaling pathway, VEGF signaling pathway, NF-kappa B signaling pathway, and the apoptotic signaling molecule BCL2. Through the research method of network pharmacology, this study summarized the preliminary experiments of the research group and revealed the probable mechanism of FXHJ in the treatment of heart failure to a certain extent, which provided some ideas for the development of new drugs.

4.
J AOAC Int ; 102(4): 1186-1193, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30609946

RESUMO

Background: Astragali radix is prone to be contaminated by various mycotoxins, leading to unpredictable threats on the quality and safety of Astragali radix and the health of the consumers. Therefore, the determination of multimycotoxins is imperative. Objective: To develop an efficient, sensitive, fast, and multianalyte method for detecting multimycotoxins in Astragali radix. Methods: A selective dilute-and-shoot pretreatment procedure based ultra-performance LC-tandem MS (UPLC-MS/MS) method was developed for sensitive determination of multimycotoxins in Astragali radix, including aflatoxin (AF) B1, B2, G1, G2, and ochratoxin A. The five mycotoxins were extracted by the dilute-and-shoot pretreatment procedure followed by UPLC-MS/MS detection. Types of sample extraction solvent, mobile-phase compositions and MS/MS parameters, and dilute-and-shoot conditions were optimized. Results: The optimized chromatographic and mass spectrometric conditions allowed the separation and detection of the five mycotoxins within 5 min. The validated UPLC-MS/MS method exhibited good sensitivity with LOD and LOQ lower than 0.2 and 0.5 µg/kg, respectively. RSD values for method precision were lower than 9%. Recoveries obtained were between 90.87 and 108.44% for all the analytes with RSDs of 3.96-8.03%. The developed method was applied for the detection of the 5 mycotoxins in 18 batches of Astragali radix with good determination performance and no matrix interferences. Two samples collected from Shanxi province and Neimenggu Autonomous Region in China were positive for AFB1 at 3.24 and 2.69 µg/kg, respectively. Conclusions: The dilute-and-shoot procedure allowed for the extraction of the mycotoxins with advantages of simple pretreatment, small extraction time, high selectivity and accuracy, as well as being cost effective and easy to operate without any clean-up steps. Highlights: This is the first report on dilute-and-shoot approach for mycotoxins extraction and detection in Chinese medicinal material matrixes.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Contaminação de Alimentos/análise , Micotoxinas/análise , Espectrometria de Massas em Tandem/métodos , Astragalus propinquus , Limite de Detecção
5.
Ultrasonics ; 94: 419-429, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30001853

RESUMO

The viscoelasticity of constituent materials has a significant effect on the dispersion relation of waves in viscoelastic phononic crystals (PCs). This paper extends the bi-directional evolutionary structure optimization (BESO) method to the design of viscoelastic PCs with the maximum attenuation and stiffness. The attenuation factor is calculated by the k(ω)-method, and the effective elasticity matrix of composite PCs is extracted by the homogenization theory. The inverse design of viscoelastic PCs is formulated with a topology optimization problem, which is then solved by the proposed BESO method. Generally, BESO re-distributes the material phases of viscoelastic PCs within the primitive unit cell step by step based on sensitivity analysis. The optimization process is stopped until the optimized viscoelastic PC with the maximum attenuation factor and the desirable bulk modulus is achieved. Numerical examples are systematically presented for the propagation of out-of-plane or in-plane waves, and combined out-of-plane and in-plane waves at various frequencies. Novel topological patterns of the optimized viscoelastic PCs are obtained and discussed.

6.
Sci Rep ; 7(1): 7445, 2017 08 07.
Artigo em Inglês | MEDLINE | ID: mdl-28785113

RESUMO

All-angle negative refraction (AANR) of phononic crystals and its frequency range are dependent on mechanical properties of constituent materials and their spatial distribution. So far, it is impossible to achieve the maximum operation frequency range of AANR theoretically. In this paper, we will present a numerical approach for designing a two-dimensional phononic crystal with broadband AANR without negative index. Through analyzing the mechanism of AANR, a topology optimization problem aiming at broadband AANR is established and solved by bi-directional evolutionary structural optimization method. The optimal steel/air phononic crystal exhibits a record AANR range over 20% and its refractive properties and focusing effects are further investigated. The results demonstrate the multifunctionality of a flat phononic slab including superlensing effect near upper AANR frequencies and self-collimation at lower AANR frequencies.

7.
R Soc Open Sci ; 3(12): 160625, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28083106

RESUMO

The size effects that reveal the dramatic changes of mechanical behaviour at nanoscales have traditionally been analysed for regular beam systems. Here, the method of using finite-element analysis is explored with the intention of evaluating the size effects for complex nanostructures. The surface elasticity theory and generalized Young-Laplace equation are integrated into a beam element to account for the size effects in classical Euler-Bernoulli and Timoshenko beam theories. Computational results match well with the theoretical predictions on the size effect for a cantilever beam and a cubic unit cell containing 24 horizontal/vertical ligaments. For a simply supported nanowire, it is found that the results are very close to the experimental data. With the assumption that nanoporous gold is composed of many randomly connected beams, for the first time, the size effect of such a complex structure is numerically determined.

8.
Materials (Basel) ; 9(3)2016 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-28773313

RESUMO

This paper systematically investigated the topological design of cellular phononic crystals with a maximized gap size between two adjacent bands. Considering that the obtained structures may sustain a certain amount of static loadings, it is desirable to ensure the optimized designs to have a relatively high stiffness. To tackle this issue, we conducted a multiple objective optimization to maximize band gap size and bulk or shear modulus simultaneously with a prescribed volume fraction of solid material so that the resulting structures can be lightweight, as well. In particular, we first conducted the finite element analysis of the phononic band gap crystals and then adapted a very efficient optimization procedure to resolve this problem based on bi-directional evolutionary structure optimization (BESO) algorithm in conjunction with the homogenization method. A number of optimization results for maximizing band gaps with bulk and shear modulus constraints are presented for out-of-plane and in-plane modes. Numerical results showed that the optimized structures are similar to those obtained for composite case, except that additional slim connections are added in the cellular case to support the propagation of shear wave modes and meanwhile to satisfy the prescribed bulk or shear modulus constraints.

9.
J Med Virol ; 87(8): 1436-40, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25952001

RESUMO

Since 1997, more and more cases of the infectious H5N1 avian influenza virus (AIV) in humans have been reported all over the world but the transmission of H5N1 avian influenza virus to stray cats has been little demonstrated. The objective of this pilot investigation was to determine the prevalence of H5N1 AIV antibodies in stray cats in eastern China where is the dominant enzootic H5N1 highly pathogenic avian influenza virus (HP AIV). A total of 1,020 nasal swab and 1,020 serum samples were collected and tested. Evidence of HPAI H5N1 virus antibodies was present in two of the 1,020 serum samples that were positive by HI assay and NT assay, respectively. The results imply little transmission and that the Clade 2.3.2 HPAIV H5N1 infections in poultry did not significantly affect the rural animal shelters or suburban environment in eastern China. In future studies, these results can be used as baseline seroepidemiological levels for H5N1 AIV among cats in China.


Assuntos
Anticorpos Antivirais/sangue , Doenças do Gato/epidemiologia , Doenças do Gato/virologia , Virus da Influenza A Subtipo H5N1/imunologia , Infecções por Orthomyxoviridae/veterinária , Animais , Gatos , China/epidemiologia , Infecções por Orthomyxoviridae/epidemiologia , Infecções por Orthomyxoviridae/virologia , Projetos Piloto , Estudos Soroepidemiológicos
10.
Huan Jing Ke Xue ; 31(8): 1775-83, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-21090292

RESUMO

Wujingang region was taken as the study area to explore the relationship between landscape pattern and river water quality. Remote sensing image was interpreted and buffer zones were constructed, and then landscape patterns characterized by land-use patterns and five selected landscape metrics including Number of patches (NP), Area-weighted mean patch fractal dimension (FRAC _AM), Shannon's diversity index (SHDI), Shannon' s evenness index (SHEI), Contagion index (CONTAG) in each buffer zone were obtained. By employing the correlation analysis between the landscape pattern and river water quality, the results indicated that the river water quality was influenced by landscape pattern. The percentage of built-up area was positively related with water quality indicators, demonstrating that the percentage of built-up area was one of the key factors affecting the river water quality, while the percentage of cultivated land showed a negative relationship. Furthermore, the relationships between the selected five landscape metrics and water quality were also revealed. SHDI and SHEI were significantly positively related with water quality indicators, while CONTAG showed the opposite relationship.


Assuntos
Produtos Agrícolas/crescimento & desenvolvimento , Monitoramento Ambiental , Arquitetura de Instituições de Saúde , Água Doce/análise , Poluentes Químicos da Água/análise , China , Resíduos Industriais , Rios
11.
Ying Yong Sheng Tai Xue Bao ; 19(7): 1522-8, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18839914

RESUMO

Aiming at the potential catastrophic characteristics of coastal city under rapid urbanization, and based on the P-S-R (pressure-state-response) framework and catastrophe progression method (CPM), this paper developed a catastrophe model for the assessment of urban eco-security, and assessed the ecological security status (ESS) of Xiamen City from 1996 to 2006. The results showed that from 1996 to 1998, the ESS had an improving trend, revealing that the rapid urbanization in this period had less effect on the regional eco-security. From 1998 to 2001, owing to the large-scale and profound ecological disturbances from sea reclamation and rapid population increase that far overstepped the ecological remediation ability of Xiamen City's ecosystem itself, the ESS declined rapidly. From 2001 to 2006, the ESS improved again, because of the significant strategic transformation of Xiamen City from its original island-based configuration to a new eco-city with larger bay area. In 2006, the security of Xiamen ecosystem pressure remained at grade III, suggesting that this city still had a potential risk of ecological security. According to our research results, CPM revealed the catastrophic effects of large development projects on ecosystems when a single index was at extreme, and offset the drawbacks of existing methods in this regard. CPM also had less subjectivity in weight evaluation and less uncertainty in estimating the standard of security, and consequently, could evaluate the status and development trend of urban eco-security exactly.


Assuntos
Ecologia/métodos , Ecossistema , Urbanização , China , Cidades , Conservação dos Recursos Naturais/métodos , Ecologia/normas , Modelos Teóricos , Medição de Risco
12.
Ying Yong Sheng Tai Xue Bao ; 19(12): 2699-705, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19288726

RESUMO

With the Rosebush Wetland Park in Changzhou as a case, a new type water supplement mode for urban wetland park, i.e., "vertical-flow plus horizontal-flow", was constructed, and its effects in water purification, ecology, and economic advantages were evaluated. The results showed that this water supplement mode could not only improve the landscape of the water bodies in urban wetland park, but also enhance their quality, making it satisfy the requirement for human full-body exposure. Furthermore, the operation cost of the mode was as lower as 5%-25% of direct municipal pipe-water supply and other routine technique solutions, suggesting that this water supplement mode had potential positive ecological effects and economic advantages.


Assuntos
Cidades , Purificação da Água/economia , Purificação da Água/métodos , Abastecimento de Água/análise , Áreas Alagadas , China , Ecossistema
13.
Zhongguo Zhen Jiu ; 25(1): 41-2, 2005 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-16309155

RESUMO

OBJECTIVE: To understand clinical application of syndrome differentiation and treatment of meridians in acupuncture and moxibustion. METHODS: Collect and collate the literature about syndrome differentiation and treatment of meridians in Chinese Acupuncture & Moxibustion, Acupuncture Research, Shanghai Journal of Acupuncture & Moxibustion (Clinic edition B in 2003) and Clinical Journal of Acupuncture and Moxibustion from 2001 to 2003. RESULTS: Less literature about syndrome differentiation and treatment of meridians are involved, only accounting for 5.69% (163/2 864); and the literature about both treatment based on meridians and treatment based on syndrome differentiation of meridians only accounted for 1.22% (35/2 864). CONCLUSION: At present, clinically acupuncture and moxibustion do not play attention to treatment based on syndrome differentiation of meridians.


Assuntos
Meridianos , Moxibustão , Pontos de Acupuntura , Terapia por Acupuntura , China , Humanos
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