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1.
Neurotox Res ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36595163

RESUMO

Cerebral ischemia/reperfusion (I/R) injury is the main cause of death following trauma. The neuroprotective effect of sevoflurane (Sev) has been implicated in cerebral I/R injury. However, the mechanisms remain elusive. In this study, we aimed to explore its function in PC12 exposed to oxygen-glucose deprivation/reoxygenation (OGD/R) and in rats challenged with I/R. Sev pretreatment reduced the damage of PC12 cells after OGD/R treatment. Moreover, Sev pretreatment ameliorated neurobehavioral deficits induced by I/R treatment, reduced brain infarct volume, and decreased apoptosis of neurons in hippocampal tissues. Sev pretreatment reduced the surface expression of glutamate receptor 1 (GRIA1) in neurons, while GRIA1 reduced the neuroprotective effects of Sev pretreatment in vitro and in vivo. There was no difference in the surface expression of GRIA2 in rats with I/R and PC12 cells exposed to OGD/R. The ratio of GRIA1/GRIA2 surface expression was reduced, and calcium permeable-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (CP-AMPAR) was blocked by Sev. Together, Sev might exert beneficial effects on cerebral I/R-induced neuronal injury through inhibiting the surface expression of GRIA1 and blocking CP-AMPAR.

2.
Neurotox Res ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36595162

RESUMO

Contrast-induced encephalopathy (CIE) following angiography, though not often and reversible, can in some cases lead to permanent neurological dysfunction. To identify how neuroinflammation is involved in CIE, we investigated microglia responses to a bolus injection of ioversol in the internal carotid artery (ICA) in rats. MicroCT scanning indicated that the injected ioversol was cleared from the rat's brain within 25 min. However, proinflammatory activated and significantly increased microglia were found in the rat occipital cortex at 1 day, and the number of blood vessel-associated microglia was still significantly higher at 3-day post-injection, compared with sham- and PBS-treated rats. Moreover, significantly upregulated malondialdehyde (MDA), downregulated superoxide dismutase (SOD) levels, and elevated proinflammatory cytokines were observed in the brain of rats treated with ioversol. Ioversol administration decreased cell viability of primarily cultured microglia and induced significant proinflammatory activation. Furthermore, ioversol remarkably upregulated astrocytic aquaporin (AQP) 4 expression in the rats brain, and transwell cultures showed significantly enhanced microglia migrating to ioversol-treated endothelial cells. Immediate injection of edaravone dexborneol, a novel antioxidative drug, after ioversol injection effectively rescued ioversol-induced neuroinflammation. Together, these findings suggest that ioversol induced neuroinflammation and oxidative stress in the brain via microglia activation in a direct and indirect manner, which might contribute to the pathogenesis of CIE.

3.
BMC Cancer ; 23(1): 88, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36698118

RESUMO

BACKGROUND: Magnetic resonance imaging (MRI) performs well in the locoregional assessment of extranodal nasal-type NK/T-cell lymphoma (ENKTCL). It's important to assess the value of multi-modal MRI-based radiomics for estimating overall survival (OS) in patients with ENKTCL. METHODS: Patients with ENKTCL in a prospectively cohort were systemically reviewed and all the pretreatment MRI were acquisitioned. An unsupervised spectral clustering method was used to identify risk groups of patients and radiomic features. A nomogram-revised risk index (NRI) plus MRI radiomics signature (NRI-M) was developed, and compared with the NRI. RESULTS: The 2 distinct type I and II groups of the MRI radiomics signatures were identified. The 5-year OS rates between the type I and type II groups were 87.2% versus 67.3% (P = 0.002) in all patients, and 88.8% versus 69.2% (P = 0.003) in early-stage patients. The discrimination and calibration of the NRI-M for OS prediction demonstrated a better performance than that of either MRI radiomics or NRI, with a mean area under curve (AUC) of 0.748 and 0.717 for predicting the 5-year OS in all-stages and early-stage patients. CONCLUSIONS: The NRI-M model has good performance for predicting the prognosis of ENKTCL and may help design clinical trials and improve clinical decision making.


Assuntos
Linfoma Extranodal de Células T-NK , Linfoma de Células T , Humanos , Prognóstico , Imageamento por Ressonância Magnética/métodos , Nomogramas , Medição de Risco , Estudos Retrospectivos , Linfoma Extranodal de Células T-NK/diagnóstico por imagem , Linfoma Extranodal de Células T-NK/patologia
4.
Artigo em Inglês | MEDLINE | ID: mdl-36658098

RESUMO

Solid-state lithium batteries are promising and safe energy storage devices for mobile electronics and electric vehicles. In this work, we report a facile in situ polymerization of 1,3-dioxolane electrolytes to fabricate integrated solid-state lithium batteries. The in situ polymerization and formation of solid-state dioxolane electrolytes on interconnected carbon nanotubes (CNTs) and active materials is the key to realizing a high-performance battery with excellent interfacial contact among CNTs, active materials and electrolytes. Therefore, the electrodes could be tightly integrated into batteries through the CNTs and electrolyte. Electrons/ions enable full access to active materials in the whole electrode. Electrodes with a low resistance of 4.5 Ω â–¡-1 and high lithium-ion diffusion efficiency of 2.5×10-11 cm2 s-1 can significantly improve the electrochemical kinetics. Subsequently, the batteries demonstrated high energy density, amazing charge/discharge rate and long cycle life.

5.
J Colloid Interface Sci ; 636: 588-601, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36669452

RESUMO

Bimetal oxide is a popular defluorinating material. Hexadecyl trimethyl ammonium bromide (CTAB) as a surfactant successfully synthesizes a novel lanthanum-zirconium phosphate to remove fluorine from groundwater. Lanthanum-zirconium phosphate at a Zr/La molar ratio of 2 exhibited a specific surface area of 455.14 m2/g with a wide pore size, which was achieved by incorporating lanthanum into materials and removing CTAB through calcination. The maximum fluoride adsorption capacity is 109.17 mg/g, which is tenfold that of mesostructured zirconium phosphate. Specifically, analysis revealed that mZrP and LamZrP2-1 were amorphous, which is consistent with HAADF-STEM. The fluoride adsorption fitted well with the pseudo-second-order equation model and Langmuir isotherm mode. LamZrP2-1 had potent anti-interference ability without PO43-. Moreover, LamZrP2-1 was reusable for at least six cycles of adsorption-desorption with little influence. The adsorption mechanism of fluoride was discussed by X-ray photoelectron spectroscopy (XPS), nuclear magnetic resonance spectroscopy (NMR) analysis, and Fourier transform infrared (FTIR) spectroscopy. Fluoride was captured by LamZrP2-1 via charge attraction, ligand exchange of different bond strengths, and ion exchange. Lanthanum-zirconium phosphate is important not only in the research and development of bimetal oxides but also in the treatment of groundwater for fluoride removal.

6.
Carbohydr Polym ; 303: 120436, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36657833

RESUMO

Infection represents a major clinical barrier that delays wound healing, while the overuse of antibiotics can lead to bacterial resistance. Hence, it is of particular important to develop a new type of dressing to combat bacterial resistance. Herein, a carbon nitride-polydopamine­silver complex (C3N4-PDA-Ag) was prepared using the photocatalyst C3N4 and silver nanoparticles (Ag NPs) to achieve a synergistic antimicrobial effect. The solution casting method was then employed to further modify the C3N4-PDA-Ag complex by compounding it with chitosan (CS), thereby forming a C3N4-PDA-Ag@CS film. The results revealed that the C3N4-PDA-Ag@CS film exhibits superior antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa compared to the CS group. The hemolysis, cytotoxicity, and in vivo implantation experiments indicated that the composite film possesses excellent in vitro and in vivo biocompatibility. In addition, the composite dressing promoted wound healing in infected mice by facilitating collagen deposition and accelerating epidermal regeneration. Collectively, the findings of this study clearly demonstrate that the C3N4-PDA-Ag@CS composite dressing has excellent antibacterial properties, biocompatibility, and enhances wound healing, thus providing a strategy for the application of photocatalytic materials for the treatment of infected wounds.


Assuntos
Infecções Bacterianas , Quitosana , Nanopartículas Metálicas , Camundongos , Animais , Prata/farmacologia , Cicatrização , Antibacterianos/farmacologia , Bandagens
7.
Bioengineering (Basel) ; 10(1)2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36671667

RESUMO

The symptoms of knee osteoarthritis (KOA) severely affect the life quality of the elderly population. Low-level laser therapy, heat therapy, and massage therapy are widely used as independent treatments for joint disorders. However, there are very limited reports of a combination of these therapies into an integrated device for KOA so far. This study aims to develop a novel hybrid therapeutic device that can meet various requirements for knee therapy. Our hybrid therapeutic device (CUHK-OA-M2) integrated with low-level laser therapy, heat therapy, and local massage therapy can effectively provide patients with KOA with relief from their clinical symptoms. A pilot test of 50 community-dwelling elderly volunteers with KOA was performed. Finally, 43 volunteers completed two treatment periods (30 days each) and two post-treatment periods (30 days each). The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores were collected and analyzed after each period. The outputs of the low-level laser, heating, and massage therapies significantly decreased the WOMAC scores in terms of pain, stiffness, function and total WOMAC after two treatment periods (p < 0.05). Although the score increased slightly after the post-treatment period, it was still lower than the baseline, indicating the treatment outcome could last for an extended period. Therefore, our CUHK-OA-M2 device, as an integrated multi-functional hybrid therapeutic device, is therapeutically significant for treating osteoarthritis symptoms on the knee joints of elderly subjects.

8.
Heliyon ; 9(1): e12845, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36704277

RESUMO

With the continuous improvement of human's requirements for temperature control suitable for living, the energy consumption of electrical appliances such as air conditioners has become a major challenge in traditional architectural design. Generally, most of the solar energy passes through the glass to enter and exit the building, but the traditional glass can hardly control the light and heat energy, causing the indoor temperature to change dramatically with the environment. Therefore, it is more urgent to develop green and efficient smart windows. Perovskite is a temperature-adaptive material, which has the ability of phase transition and can adjust its band gap for thermochromic applications. In this work, we study the perovskite-based thermochromic smart window. As a new application of perovskite, a number of experiments have been carried out. However, there is still a lack of theoretical analysis on phase transition mechanisms and crystal structure prediction. Density functional theory (DFT) calculation is the most useful tool in optoelectronics, especially for perovskite crystal. Here, we extracted typical cases from published literature for analysis and comparison and summarized the crystal structure, electronic structure stability, interface engineering, and thermal characteristics employing DFT calculation We believe this work will pave the way for DFT application for the study of thermochromic perovskite.

9.
J Biotechnol ; 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36708998

RESUMO

As versatile and green biocatalysts for the asymmetric amination of ketones, the insufficient thermostability of transaminases always limits its broad application in the pharmaceutical and fine chemical industries. Here, synthetic shuffling technology was used to enhance stability of (R)-selective transaminase from Aspergillus terreus. The results showed that 30 out of 5000 mutants had improved thermostability by color-based screening method, among which mutants with residual enzyme activity higher than 50% at 45 °C for 10min were selected for further analysis. Especially, the half-inactivation temperature (T), half-life (t1/2), and melting temperature (Tm) of the best mutant M14 (M280C-H210N-M150C-F115L) were 13.7 °C, 165.8min, and 13.9 °C higher than that of the wild type (WT), respectively. M14 also exhibited a significant biocatalytic efficiency toward acetophenone and 1-acetylnaphthalene, the yield of which were 265.6% and 117.5% higher than WT, respectively. Based on molecular dynamics simulation, improved catalytic efficiency of M14 could be attributed to its increased hydrogen bonds interaction around the mutation sites. Additionally, the introduction of disulfide bond combined with above mutations has a synergistic effect on the improved protein thermostability.

10.
Anal Chem ; 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36695821

RESUMO

The accurate discrimination of single-nucleotide variants is of great interest for disease diagnosis and clinical treatments. In this work, a unique DNA probe with "Hill-type" cooperativity was first developed based on toehold-mediated strand displacement processes. Under simulation, this probe owns great thermodynamics advantage for specificity due to two mismatch bubbles formed in the presence of single-nucleotide variants. Besides, the strategies of ΔG' = 0 and more competitive strands are also beneficial to discriminate single-nucleotide variants. The feasibility of this probe was successfully demonstrated in consistent with simulation results. Due to "Hill-type" cooperativity, the probe allows a steeper dynamic range compared with previous probes. With simulation-guided rational design, the resulting probe can accurately discriminate single-nucleotide variants including nucleotide insertions, mutation, and deletions, which are arbitrarily distributed in target sequence. Two specificity parameters were calculated to quantitatively evaluate its good discrimination ability. Hence, "Hill-type" cooperativity can serve as a novel strategy in DNA probe's design for accurate discrimination of single-nucleotide variants.

11.
Sci Rep ; 13(1): 1288, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36690687

RESUMO

Toxoplasma gondii can infect the host brain and trigger neuroinflammation. Such neuroinflammation might persist for years if the infection is not resolved, resulting in harmful outcomes for the brain. We have previously demonstrated the efficacy of immunotherapy targeting the programmed cell death protein 1 (PD-1) pathway on clearance of Toxoplasma tissue cysts. We aimed to test whether parasite clearance would lead to the resolution of neuroinflammation in infected brains. We established chronic Toxoplasma infection in BALB/c mice using the cyst-forming Prugniaud strain. Mice then received αPD-L1 or isotype control antibodies. After completion of the therapy, mice were euthanized six weeks later. The number of brain tissue cysts, Toxoplasma-specific CD8 + T cell proliferation and IFN-γ secretion, serum cytokine and chemokine levels, and CNS inflammation were measured. In αPD-L1-treated mice, we observed reduced brain tissue cysts, increased spleen weight, elevated IFN-γ production by antigen-specific CD8 + T cells, and a general increase in multiple serum cytokines and chemokines. Importantly, αPD-L1-treated mice displayed attenuation of meningeal lymphocytes, reactive astrocytes, and C1q expression. The reduction in inflammation-related proteins is correlated with reduced parasite burden. These results suggest that promoting systemic immunity results in parasite clearance, which in turn alleviates neuroinflammation. Our study may have implications for some brain infections where neuroinflammation is a critical component.


Assuntos
Toxoplasma , Camundongos , Animais , Doenças Neuroinflamatórias , Receptor de Morte Celular Programada 1/metabolismo , Citocinas/metabolismo , Encéfalo/metabolismo , Quimiocinas/metabolismo , Inflamação/metabolismo , Camundongos Endogâmicos BALB C , Imunoterapia
12.
Eur J Med Res ; 28(1): 34, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36653862

RESUMO

OBJECTIVES: To compare the detection rates of [68Ga]Ga-FAPI-04 PET MRI/CT vs. [18F]-FDG PET MRI/CT in gastric cancer. METHODS: An extensive librarian-led literature search of PubMed, Embase, Web of Science, the Cochrane Central Library, and ClinicalTrials.gov was performed. The primary outcomes were sensitivity in patient-based evaluations, detection of lymph node metastases, and peritoneal involvement. RESULTS: Five studies, including 148 participants, were analyzed. [68Ga]Ga-FAPI-04 PET MRI/CT has a comparatively high sensitivity in patient-based evaluations compared with [18F]-FDG PET MRI/CT (risk difference = 0.16, 95% CI 0.09-0.22, P < 0.00001). The [68Ga]Ga-FAPI-04 PET MRI/CT group has a comparatively higher sensitivity in detecting lymph node metastases (RR = 0.15, 95% CI 0.01-0.29, P = 0.04), peritoneal involvement (RR = 0.55, 95% CI 0.38-0.72, P < 0.00001) in gastric cancer than [18F]-FDG PET MRI/CT group. CONCLUSIONS: This systematic review confirmed the advantage of [68Ga]Ga-FAPI-04 PET MRI/CT in gastric cancer. [68Ga]Ga-FAPI-04 PET MRI/CT was superior to [18F]-FDG PET MRI/CT in detecting the primary tumor, lymph node metastases, and peritoneal metastases. More studies are needed for the sensitivity and specificity of [68Ga]Ga-FAPI-04 PET MRI/CT in different pathological types of gastric cancer.


Assuntos
Carcinoma , Neoplasias Gástricas , Humanos , Fluordesoxiglucose F18 , Radioisótopos de Gálio , Neoplasias Gástricas/diagnóstico por imagem , Metástase Linfática/diagnóstico por imagem , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Imageamento por Ressonância Magnética
13.
Brain Cogn ; 166: 105951, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36680856

RESUMO

Bribe-taking decision is a social dilemma for individuals: the pursuit of economic self-interest vs. compliance with social norms. Despite the well-known existence of the conflict in deciding whether to accept bribes, little is known about its neural responses. Using functional near-infrared imaging (fNIRS) technology and the bribe-taking decision game (economic gambling game as a control condition), the current study dissociated the neural correlates of the different motivations in the bribery dilemma, as well as the inhibitory effect of social norms on bribery and its underlying brain mechanisms in supra-cortical regions. Findings revealed that if individuals are more motivated by economic interest, rejecting money (vs. accepting money) accompanies higher activity in the dorsolateral prefrontal cortex (DLPFC) and frontopolar cortex (FPC), which reflects impulse inhibition and decision evaluation; whereas, if individuals are more consider social norms, their DLPFC is more active when they accept bribes (vs. reject bribes), which reflects their fear of punishment. Additionally, the key brain region where social norms inhibit bribery involves the left DLPFC. The current findings contribute to the literature on the neural manifestations of corrupt decisions and provide some insights into the anti-corruption movement.

14.
Stem Cell Rev Rep ; 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36609903

RESUMO

BACKGROUND: Ovarian ageing causes endocrine disturbances and the degeneration of systemic tissue and organ functions to seriously affect women's physical and mental health, and effective treatment methods are urgently needed. Based on our previous studies using juvenile rhesus monkey bone marrow mesenchymal stem cells (BMMSCs) to treat ovarian ageing in rhesus monkey, we found that BMMSCs improved ovarian structure and function. This study continues to explore the mechanism by which BMMSCs reversed granulosa cell (GC) ageing. METHODS: A GC ageing model and coculture system of BMMSCs were established, changes in the level of the N6-methyladenosine (m6A) methylation modification were detected, m6A-modified RNA immunoprecipitation sequencing (MeRIP-seq) were performed, correlations between m6A peaks and mRNA expression were determined, and the expression of hub genes was identified using Q-PCR, immunofluorescence staining, and western blot. RESULTS: Our results showed that H2O2 successfully induced GC ageing and that BMMSCs reversed measures of GC ageing. BMMSCs increased the expression of the FTO protein and reduced the overall level of m6A. We identified 797 m6A peaks (348 hypomethylated and 449 hypermethylated peaks) and 817 differentially expressed genes (DEGs) (412 upregulated and 405 downregulated) after aged GCs were cocultured with BMMSCs, which significantly associated with ovarian function and epigenetic modification. The epigenetic repressive mark and important cell cycle regulator lysine demethylase 8 (KDM8) was downregulated at both the mRNA and protein levels, histone H3 was upregulated in aged GCs after BMMSC coculture, and KDM8 was upregulated after FTO was inhibited through FB23. CONCLUSIONS: Our study revealed an essential role for m6A in BMMSCs in reversing GC ageing, and FTO regulated KDM8 mediates histone H3 changes may as a novel regulatory mechanism in BMMSCs to reverse GC ageing.

15.
Adv Mater ; : e2209956, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36656747

RESUMO

Encryption technologies are essential for information security and product anti-counterfeiting, but they are typically restricted to planar surfaces. Encryption on complex 3D objects offers great potential to further improve security. However, it is rarely achieved owing to the lack of encoding strategies for non-planar surfaces. Here, we report an approach to directly encrypt on a 3D printed object employing orthogonal photo-chemistry. In this system, visible light photo-chemistry is used for 3D printing of a hydrogel and ultraviolet light is subsequently employed to activate its geometrically complex surface through the dissociation of ortho-nitrobenzyl ester units in a spatio-selective manner for information coding. Our approach offers a new way for more reliable encryption and the underlying orthogonal photo-chemistry can be extended towards functional modification of 3D printed products beyond information protection. This article is protected by copyright. All rights reserved.

16.
Clin Exp Optom ; : 1-7, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36634626

RESUMO

CLINICAL RELEVANCE: Suspected optic disc swelling is a common presentation in children. The delineation between true optic disc swelling and pseudopapilloedema, its common masquerade, requires careful evaluation. A streamlined pathway is required to avoid unnecessary investigations. BACKGROUND: Papilloedema requires urgent neuroimaging, however, perceived optic disc swelling is not always true papilloedema. This study aims to investigate the outcome of referrals for optic disc swelling and formulate features that may assist in investigation and diagnosis. METHODS: A retrospective review of referrals for optic disc swelling to the Queensland Children's Hospital, Australia, between January 2014 and June 2020 was undertaken. RESULTS: Four hundred and ten children were referred for optic disc swelling. Sixty-six patients were confirmed with optic disc swelling, and 344 patients had pseudopapilloedema. The average age was 10.10 ± 3.57 and 9.90 ± 3.50 years, respectively. The most common aetiology of optic disc swelling was idiopathic intracranial hypertension (n = 25). Optic disc drusen constituted the majority of pseudopapilloedema (n = 239) and the remainder were crowded/tilted discs (n = 105). True optic disc swelling patients were more likely to experience headache (OR = 8.68, p < 0.01) and visual disturbance (OR = 2.14, p = 0.03). B-scan was the most sensitive for the detection of optic disc drusen (100%), followed by optical coherence tomography (70.38%) and fundus autofluorescence (44.86%). The retinal nerve fibre layer thickness was significantly thicker in true optic disc swelling compared to pseudopapilloedema (p < 0.01). Twenty-two (33.33%) true optic disc swelling patients and 33 (9.59%) pseudopapilloedema patients underwent neuroimaging prior to ophthalmology review. CONCLUSIONS: Suspected optic disc swelling in children is most likely pseudopapilloedema. Referrals should include neurological assessment, visual acuity, fundus photography, and optical coherence tomography to assist in the triage for ophthalmic review. Neuroimaging prior to ophthalmic review should be discouraged for children without neurological symptoms.

17.
Magn Reson Imaging ; 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36603780

RESUMO

OBJECTIVE: This study aims to explore the diagnostic performance of amide proton transfer (APT) imaging combined with diffusion kurtosis imaging (DKI) in differentiating stage Ia endometrial carcinoma (EC) and endometrial polyps (EP). METHODS: All patients were scanned with APT and DKI sequences with 3.0 T MRI before surgery. The MRI data of 32 patients with histopathologically confirmed stage Ia EC and 17 patients with EP were retrospectively analyzed. Amide proton transfer, mean kurtosis (MK) and mean diffusivity (MD) values were measured. ROC curves were employed to evaluate the efficiency of differentiating and diagnosing stage Ia EC and EP, followed by the Delong test to compare the differences between the areas under the curve (AUCs) of each parameter. Additionally, the Pearson correlation analysis was performed to assess the correlation between APT values and MK and MD. RESULTS: The measured APT, MK, and MD values of the patients with stage Ia EC were 2.609 ±â€¯0.504%,0.641 ±â€¯0.113 and 0.904(0.816, 1.108) µm2/ms, while those of patients with EP were1.909 ±â€¯0.418%, 0.495 ±â€¯0.069, and 1.650 (1.458, 1.815) µm2/ms. The AUCs of APT, MK, MD, MK + MD, and APT+MK + MD in differentiating stage Ia EC and EP were.850, 0.879, 0.893, 0.930 and 0.976, respectively. The AUCs of APT+MK + MD were significantly higher than the AUCs of APT or MK (P < 0.05). The APT value was weakly and positively correlated with the MK value (r = 0.299, P = 0.037), while the APT value was moderately and negatively correlated with the MD value (r = -0.520, P < 0.001). CONCLUSION: Both APT and DKI effectively differentiated stage Ia EC and EP; however, when combined, APT and DKI improved the ability to differentiate these diseases, boosting the value of using a combination of these modalities in clinical applications.

18.
Arch Virol ; 168(1): 17, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36593367

RESUMO

Bacillus subtilis strains play a pivotal role in the fermentation industry. B. subtilis phages can cause severe damage by infecting bacterial cells used in industrial fermentation processes. In this work, we isolated and characterized a Bacillus subtilis-infecting phage, termed phi18. Transmission electron microscopy revealed that phage phi18 particles have typical myovirus morphology, with an icosahedral head connected to a contractile tail. Genomic analysis revealed that the phage genome is a linear double-stranded DNA molecule of 147,298 bp with terminal redundancy of 14,434 bp, and 226 protein coding genes and four tRNA genes were predicted in the genome. Phage-resistant mutants were selected from a mariner transposon-insertion library of B. subtilis 168 in which two bacterial genes, tagE and pgcA, which are required for the glycosylation of wall teichoic acid (WTA), were found to be disrupted, suggesting that WTA is the receptor for phage phi18. Comparative genomic analysis showed that phage phi18 is a new member of the genus Okubovirus of the family Herelleviridae. Finally, general characteristics of the phage-resistant mutants, including biofilm formation, growth, and sporulation, were examined. The results showed that the phage-resistant mutants grew as rapidly as the parental strain B. subtilis 168 at 42 °C, suggesting that these phage-resistant mutants may be used as starters in fermentation processes.


Assuntos
Fagos Bacilares , Bacteriófagos , Bacillus subtilis/genética , Genômica , Bacteriófagos/genética , Genoma Viral , Glicosilação
19.
Plast Reconstr Surg ; 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36626597

RESUMO

BACKGROUND: The unpredictable and unstable tissue retention rate of autologous fat grafting remains an obstacle faced by plastic surgeons. Our previous study using fat grafting mice model with donor site showed ASCs infiltration in recipient site was delayed, leading to poor regeneration and lower retention. Thus, the mechanism behind the differential infiltration of ASCs required to be explored. METHODS: Firstly, we locally injected CXCL12 or CXCR4 inhibitor AMD3100 in recipient or donor site respectively (CXCL12+AMD3100-Group, CXCL12-AMD3100+Group, and CXCL12+AMD3100+Group). We compared the migration of ASCs, adipose regeneration and long-term retention. Next, we explored the role of angiogenesis using a Normal/Ischemic mice model in which we test the expression of CXCL12/CXCR4, migration of ASCs, and adipose regeneration. RESULTS: Blocking CXCL12 in donor site using AMD3100 (CXCL12-AMD3100+, and CXCL12+AMD3100+Group) could accelerate ASCs infiltration, promote adipose regeneration and long-term retention (p<0.05) compared with the other groups. CXCL12 and its receptor CXCR4 expressed higher in Normal than in Ischemic adipose tissue; consistently, there were more ASCs infiltrating Normal than Ischemic adipose tissue early after surgery (p<0.05). CONCLUSION: Early angiogenesis is essential for CXCL12 in promoting ASCs infiltration, improving adipose tissue repair in recipient site and potentiating long-term fat retention rate.

20.
FEBS Lett ; 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36650560

RESUMO

Autophagy and Hippo signalling pathways both play important roles in cell homeostasis and are often involved in tumourigenesis. However, the crosstalk between these two signal pathways in response to stress conditions, such as nutrient deficiency, is incompletely understood. Here, we show that vesicular localised coiled-coil domain containing 115 (CCDC115) inhibits autophagy as well as Hippo signalling pathway under starvation. Moreover, we show that CCDC115 interacts with the HOPS complex. This interaction competes with STX17, thus inhibiting the fusion of autophagosomes with lysosomes. Hence, CCDC115 inhibits the autophagic degradation of yes-associated protein (YAP), thereby promoting cell proliferation in nutrient-restricted situation.

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