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1.
ISA Trans ; 119: 172-183, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33676740

RESUMO

Ridge extraction is an effective tacholess order tracking technique for the fault detection of bearings under time-varying speed conditions. Cost function ridge detection (CFRD) is the most widely used ridge detection method. However, improper bandwidth selection and unreasonable cost function construction significantly restrict the performance of the CFRD. To address the two shortcomings of the CFRD, an improved CFRD (ICFRD) method is firstly proposed in this paper. The ICFRD integrates an adaptive search bandwidth determination technique that varies the search region with signal signatures, as well as a novel cost function that comprehensively considers the trade-off between ridge amplitude and smoothness. An iterative characteristic ridge extraction (ICRE) strategy is then presented based on the ICFRD to extract multiple characteristic ridges in a time-frequency plane automatically. The average frequency ratios between the extracted characteristic ridges are calculated to estimate bearing fault characteristic orders and therefore detect bearing faults. The performance of the proposed method was tested using simulated signals and experimental vibration signals collected from a machinery test rig. Results show that the ICRE outperforms the conventional CFRD in terms of detecting bearing faults under variable speed conditions. The average relative errors between the extracted instantaneous frequencies and the theoretical ones of the ICRE are 0.85%, 2.11% and 0.63% for inner race fault, outer race fault, and healthy bearing vibration signal, respectively. These values are much smaller than the results of using the CFRD.

2.
J Clin Invest ; 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34762600

RESUMO

Early initiation of antiretroviral therapy (ART) in acute HIV infection (AHI) is effective in limiting seeding of the HIV viral reservoir, but little is known about how the resultant decreased antigen load affects long-term antibody development after ART. We report here that Env-specific plasma antibody levels and antibody-dependent cellular cytotoxicity (ADCC) increased during the first 24 weeks of ART and correlated with antibody levels persisting after 48 weeks of ART. Participants treated in AHI stage 1 had lower Env-specific antibodies levels and ADCC activity on ART than those treated later. Importantly, participants who initiated ART after peak viremia in AHI developed elevated cross-clade ADCC responses detectable one year after ART initiation even though clinically undetectable viremia was reached by 24 weeks. These data suggest that there is more germinal center activity in the later stages of AHI and that antibody development continues in the absence of detectable viremia during the first year of suppressive ART. Development of therapeutic interventions that can enhance earlier development of germinal centers in AHI and antibodies after ART initiation could provide important protection against the viral reservoir that is seeded in early treated individuals.

3.
Front Neurosci ; 15: 728874, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34764850

RESUMO

Diabetes with high blood glucose levels may damage the brain nerves and thus increase the risk of dementia. Previous studies have shown that dementia can be reflected in altered brain structure, facilitating computer-aided diagnosis of brain diseases based on structural magnetic resonance imaging (MRI). However, type 2 diabetes mellitus (T2DM)-mediated changes in the brain structures have not yet been studied, and only a few studies have focused on the use of brain MRI for automated diagnosis of T2DM. Hence, identifying MRI biomarkers is essential to evaluate the association between changes in brain structure and T2DM as well as cognitive impairment (CI). The present study aims to investigate four methods to extract features from MRI, characterize imaging biomarkers, as well as identify subjects with T2DM and CI.

4.
Biol Trace Elem Res ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34791623

RESUMO

We have clarified that malnutrition risk evaluated by the Royal Free Hospital-Nutritional Prioritizing Tool (RFH-NPT) is prevalent in patients with cirrhosis. Mineral elements (zinc, iron, magnesium, copper, manganese, and calcium) are micronutrients essential for versatile physiological processes and cellular bioactivities. However, the association between these trace elements and integral nutritional status is unclear in decompensated cirrhotics. We collected blood samples from hospitalized patients with cirrhosis, and serum trace element concentrations were examined by inductively coupled plasma mass spectrometry. Association of trace element levels with high malnutrition risk was determined by multivariate logistic regression model. Sera from 141 patients with decompensated cirrhosis were analyzed for a total of six trace element concentrations. No significant differences were observed between high and low/moderate RFH-NPT malnutrition risk groups with the exception of zinc. The serum zinc concentrations were significantly decreased in patients at high malnutrition risk when compared to low/moderate subjects (57.9 vs 68.1 µg/dL, P = 0.006). In terms of receiver operating characteristics curve, zinc < 64 µg/dL represented best discriminative capability with an area of 0.635 (95% CI: 0.542, 0.728). Patients in the group with zinc < 64 µg/dL had elevated RFH-NPT and MELD score, higher proportion of Child-Pugh class C and ascites, higher CRP, lower albumin and sodium than in the group with zinc ≥ 64 µg/dL. Zinc < 64 µg/dL was an independent risk factor for high malnutrition risk. Low levels of serum zinc referring to less than 64 µg/dL were associated with poor integral nutritional status in cirrhosis.

6.
Colloids Surf B Biointerfaces ; 209(Pt 1): 112184, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34741910

RESUMO

Single-base mutations of Factor V Leiden G1691A and Prothrombin gene G20210A are the main genetic risk factors for inherited thrombotic tendency. The establishment for rapid and efficient detection method is of great significance to the prevention of venous thrombosis. In this work, a multiplexed, highly sensitive and regenerable surface plasmon resonance imaging (SPRi) sensor is described to identify and detect the two pathogenic genes by fixing probes in one-step. The probes are fixed by ployA, which is a simpler, faster and lower cost modification method compared with traditional thiol (-SH). PolyA-DNA-AuNPs is used to amplify the signal to improve sensitivity. The detection limit of the sensor is 8 pM, and it has a wide dynamic range between 8 pM and 100 nM and a good linear relationship between 8 pM to 50 pM. The equilibrium dissociation constant (KD) of 3.0 (± 0.3) pM indicates a high binding capacity. Based on the advantages of high-throughput detection, the SPRi chip can simultaneously identify and detect two genes related to thrombotic Diseases. In addition, more than 90% signal intensity can still be obtained on the surface of the chip after being regenerated of 25 times, indicating that this SPRi sensor has good stability and reproducibility. The established SPRi sensor has the advantages of high-throughput, high-sensitivity, label-free and no need for amplification, which is expected to become an effective technical means for real-time online detection of gene point mutations, and can be extended to detect and quantify a wider range of DNA mutation diseases.

7.
Org Biomol Chem ; 19(45): 9986-9995, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34755156

RESUMO

In this work we report the binding properties of rotaxane 1 towards a series of tetraalkylammonium salts of Cl-, OCN- and NO3- anions in acetone and a CHCl3/MeOH solvent mixture. We used 1H NMR titrations and Isothermal Titration Calorimetry (ITC) experiments to monitor and analyze the binding processes. We compared the obtained results with those previously described by us in chloroform solution. In acetone solution, the determined binding constants for the 1 : 1 complexes were 1 to 3 orders of magnitude larger than those measured in chloroform, a less competitive solvent for hydrogen-bonding. The thermodynamic signatures of the binding processes in acetone, determined by ITC experiments, revealed favorable enthalpic and entropic contributions having similar magnitudes. These results suggested that solvation/desolvation processes in acetone play a significant role in the binding processes. Conversely, the addition of just 5% of methanol to chloroform solutions of 1 significantly reduces the magnitude of the binding constants of all studied ion-pairs. In this solvent mixture, the entropy term is also favorable but it does not compensate the experienced loss of binding enthalpy. Moreover, in acetone solution, the addition of the Cl- and OCN- tetraalkylammonium salts in excess (more than 1 equiv.) led to the immediate appearance of 2 : 1 complexes. Related high-stoichiometry complexes are not observed in the solvent mixture (CHCl3/MeOH 95/5). In chloroform, a large excess of the salt (> 6 equiv.) is required for its formation. From the analysis of the obtained binding data we infer that, in acetone and in CHCl3/MeOH mixture, the formed complexes are mainly anionic.

8.
J Am Chem Soc ; 143(47): 19919-19927, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34783547

RESUMO

The microenvironment in which the catalysts are situated is as important as the active sites in determining the overall catalytic performance. Recently, it has been found that nanoparticle (NP) surface ligands can actively participate in creating a favorable catalytic microenvironment, as part of the nanoparticle/ordered-ligand interlayer (NOLI), for selective CO2 conversion. However, much of the ligand-ligand interactions presumed essential to the formation of such a catalytic interlayer remains to be understood. Here, by varying the initial size of NPs and utilizing spectroscopic and electrochemical techniques, we show that the assembly of NPs leads to the necessary ligand interactions for the NOLI formation. The large surface curvature of small NPs promotes strong noncovalent interactions between ligands of adjacent NPs through ligand interdigitation. This ensures their collective behavior in electrochemical conditions and gives rise to the structurally ordered ligand layer of the NOLI. Thus, the use of smaller NPs was shown to result in a greater catalytically effective NOLI area associated with desolvated cations and electrostatic stabilization of intermediates, leading to the enhancement of intrinsic CO2-to-CO turnover. Our findings highlight the potential use of tailored microenvironments for NP catalysis by controlling its surface ligand interactions.

9.
Int J Mol Sci ; 22(21)2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34768990

RESUMO

Chemical exchange saturation transfer (CEST) MRI is a promising molecular imaging tool which allows the specific detection of metabolites that contain exchangeable amide, amine, and hydroxyl protons. Decades of development have progressed CEST imaging from an initial concept to a clinical imaging tool that is used to assess tumor metabolism. The first translation efforts involved brain imaging, but this has now progressed to imaging other body tissues. In this review, we summarize studies using CEST MRI to image a range of tumor types, including breast cancer, pelvic tumors, digestive tumors, and lung cancer. Approximately two thirds of the published studies involved breast or pelvic tumors which are sites that are less affected by body motion. Most studies conclude that CEST shows good potential for the differentiation of malignant from benign lesions with a number of reports now extending to compare different histological classifications along with the effects of anti-cancer treatments. Despite CEST being a unique 'label-free' approach with a higher sensitivity than MR spectroscopy, there are still some obstacles for implementing its clinical use. Future research is now focused on overcoming these challenges. Vigorous ongoing development and further clinical trials are expected to see CEST technology become more widely implemented as a mainstream imaging technology.

10.
Environ Sci Technol ; 55(22): 15236-15245, 2021 11 16.
Artigo em Inglês | MEDLINE | ID: mdl-34724783

RESUMO

The risk of human exposure to particulate novel brominated flame retardants (NBFRs) in the atmosphere has received increasing attention from scientists and the public, but currently, there is no reliable approach to predict the intake of these compounds on the basis of their size distribution. Here, we develop a reliable approach to predict the size-dependent inhalation intake of particulate NBFRs, based on the gas/particle (G/P) partitioning behavior of the NBFRs. We analyzed the concentrations of eight NBFRs in 363 size-segregated particulate samples and 99 paired samples of gaseous and bulk particles. Using these data, we developed an equation to predict the G/P partitioning quotients of NBFRs in particles in different size ranges (KPi) based on particle size. This equation was then successfully applied to predict the size-dependent inhalation intake of particulate NBFRs in combination with an inhalation exposure model. This new approach provides the first demonstration of the effects of the temperature-dependent octanol-air partitioning coefficient (KOA) and total suspended particle concentration (TSP) on the intake of particulate NBFRs by inhalation. In an illustrative case where TSP = 100 µg m-3, inhalation intake of particulate NBFRs exceeded the intake of gaseous NBFRs when log KOA > 11.4.


Assuntos
Retardadores de Chama , Atmosfera , Poeira/análise , Monitoramento Ambiental , Retardadores de Chama/análise , Éteres Difenil Halogenados/análise , Humanos
11.
Biomater Sci ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34811566

RESUMO

Cisplatin is the most widely used chemotherapeutic agent due to its efficacy in the treatment of a broad range of cancer types; while the side effects and drug resistance of cisplatin limit its clincial application. Combination therapy, which contains several types of free drugs, exhibits promising potential in clinical practice. Nevertheless, current combination chemotherapy cannot accurately deliver different drug components into a single tumor cell at the same time. Herein, we report a triple-action nanoplatinum drug based on artesunate and cantharidin to overcome the influence of pharmacokinetics and distribution variation in different drugs. The results show that the triple action nanoplatinum drug enhances ROS generation, leads to DNA damage, and inhibits DNA repair. Therefore, a high-efficiency killing effect is achieved with a triple-action platinum drug in a single tumor cell.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34820731

RESUMO

The distribution patterns and health risk assessment of nitrated polycyclic aromatic hydrocarbons (NPAHs), hydroxy polycyclic aromatic hydrocarbons (OH-PAHs), and regular 16 priority polycyclic aromatic hydrocarbons (PAHs) in sediment from the Songhua River in northeastern China were investigated in this research. During dry seasons, concentrations of 16 USEPA priority PAHs, OH-PAHs, and NPAHs were extremely high, with average values of 1220 ± 288, 317 ± 641, 2.54 ± 3.98, and 12.2 ± 22.1 ng/g (dry weight, dw). The dry period level was confirmed to be 4 times greater than the wet period concentration. Modeling with positive matrix factorization (PMF) and estimation of diagnostic isomeric ratios were applied for identifying sources, according to the positive matrix factorization model: vehicle emissions (38.1%), biomass burning (25%), petroleum source (23.4%), and diesel engines source (13.5%) in wet season as well as wood combustion (44.1%), vehicle source (40.2%), coke oven (10.8%), and biomass burning (4.9%) in the dry season. The greatest seasonal variability was attributed to high molecular weight compounds (HMW PAHs). BaP was confirmed to be 81% carcinogenic in this study, which offers convincing proof of the escalating health issues.

13.
Cancer Cell Int ; 21(1): 630, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34838013

RESUMO

BACKGROUND: Osteosarcoma is a primary malignant tumor that mainly affects children and young adults. Transmembrane emp24 trafficking protein 3 (TMED3) may be involved in the regulation of malignant cancer behaviors. However, the role of TMED3 in osteosarcoma remains mysterious. In this study, the potential biological function and underlying mechanism of TMED3 in progression of osteosarcoma was elaborated. METHODS: The expression of TMED3 in osteosarcoma was analyzed by immunohistochemical staining. The biological function of TMED3 in osteosarcoma was determined through loss-of-function assays in vitro. The effect of TMED3 downregulation on osteosarcoma was further explored by xenograft tumor model. The molecular mechanism of the regulation of TMED3 on osteosarcoma was determined by gene expression profile analysis. RESULTS: The expression of TMED3 in osteosarcoma tissues was significantly greater than that in matched adjacent normal tissues. Knockdown of TMED3 inhibited the progression of osteosarcoma by suppressing proliferation, impeding migration and enhancing apoptosis in vitro. We further validated that knockdown of TMED3 inhibited osteosarcoma generation in vivo. Additionally, ribosomal protein S15A (RPS15A) was determined as a potential downstream target for TMED3 involved in the progression of osteosarcoma. Further investigations elucidated that the simultaneous knockdown of RPS15A and TMED3 intensified the inhibitory effects on osteosarcoma cells. Importantly, knockdown of RPS15A alleviated the promotion effects of TMED3 overexpression in osteosarcoma cells. CONCLUSIONS: In summary, these findings emphasized the importance of TMED3/RPS15A axis in promoting tumor progression, which may be a promising candidate for molecular therapy of osteosarcoma.

14.
Chemosphere ; : 133052, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34826442

RESUMO

In this study, a trioctylmethylammonium chloride (N263)- tributyl phosphate (TBP)-n-octanol-sulfonated kerosene (N263-T) synergistic extraction system and an N263-n-octanol-sulfonated kerosene (N263-O) system were used to treat cyanide (CN)-bearing wastewater. The extraction saturation capacity of the two systems was measured. The influences of the initial pH and phase ratio (O/A) of the two systems on extraction were compared and analyzed. Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, and slope methods were used to analyze the characteristic functional groups in the loaded organic phase, the compositions of the extracted compounds in the extraction reactions and the reaction mechanism. The results indicated that the saturated extraction capacity of the N263-T system, which was much larger than that of the N263-O system, for metal CN complex ions was 4354.31 mg/L. In addition, the N263-T system operated over a wider pH range. The extraction rates of copper (Cu), zinc (Zn), and iron (Fe) ions at pH 14 were 97.4%, 99.1%, and 87.2%, respectively. There was a strong compatibilization effect of TBP on the extraction system. The extraction efficiency of the N263-T system for metal CN complex ions was higher than that of the N263-O system when O/A = 0.4. The saturated loaded N263-T and N263-O systems were stripped by 1 mol/L NaOH +2 mol/L NaSCN solution at O/A = 3. The metal ion concentration in the stripping solution could be enriched to 11996.6 and 8913.3 mg/L for the N263-T and N263-O systems, respectively. During the extraction process, the binding ratios of N263 cations to Cu(CN)32-, Zn(CN)42-, and Fe(CN)63- were 2:1, 2:1, and 3:1, respectively. The binding ratios of TBP to Cu(CN)32-, Zn(CN)42-, and Fe(CN)63- in wastewater were 3:1, 4:1, and 6:1, respectively. The PO group in TBP was linked to the CN group of the metal CN complex ion by hydrogen bonds using the water molecule as a bridge to form a supramolecular anion group, which entered into the organic phase and combined with the N263 cation under the action of ion association.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34772500

RESUMO

Surface interface regulation is an important research content in the field of heterogeneous catalysis. To improve the interface interaction between the active component and matrix, tremendous efforts have been dedicated to tailoring the morphology, size, and structure of composite catalysts. In this work, we report a confinement strategy to synthesize a series of core-shell catalysts loaded with metal oxides on titania nanotubes (TNTs), which were applied to the selective catalytic reduction of NOx with ammonia. Interestingly, the core-shell catalyst with confinement of TNTs exhibited the remarkable activity at low temperature region, N2 selectivity and sulfur tolerance. Benefiting from the superior interfacial confinement characteristic of TNTs and Fe2O3, strong component interactions, the surface acid sites and strong oxidizability of MnOx were properly regulated, thus obtained the outstanding activity, N2 selectivity and provide chemical protection to effectively prevent SO2 poisoning. As far as the reaction mechanism, we found that the adsorption and reactivity of Lewis acid sites were the dominant factors affecting the activity in the NH3-SCR process by in situ DRIFT spectra. In general, our work provides an innovative strategy for constructing an TNTs-enwrapped nanocomposite with nano-confinement and core-shell structure to improve the low temperature SCR process.

16.
Water Res ; 207: 117780, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34731661

RESUMO

Polycyclic aromatic hydrocarbons (PAHs) as a group of toxic and carcinogenic compounds are large scale globally emitted anthropogenic pollutants mainly emitted into the atmosphere. However, atmospheric transport cannot fully explain the spatial variability of PAHs in the marine atmosphere and seawater. It is hypothesized that PAHs accumulated in seawater and ocean circulation can also influence PAHs observed in air above the ocean. In order to investigate PAHs in seawater as a potential secondary source to air, we collected paired air and seawater samples during a research cruise from China to the Antarctic in 2018-2019, covering the Pacific Ocean, the Indian Ocean, and the Southern Ocean. Summed concentrations of 28 analyzed PAHs in seawater were highest in the Pacific Ocean (4000 ± 1400 pg/L), followed by the Indian Ocean (2700 ± 1000 pg/L), and the Southern Ocean (2300 ± 520 pg/L). Three-ringed PAHs dominated the composition profile. We found that PAH levels in the Pacific and Indian Oceans were strong inversely correlated with salinity and distance to the coastline. This suggests that riverine inputs and continental discharges are important sources of PAHs to the marine environment. Derived air-seawater fugacity ratios suggest that net fluxes of PAHs were from seawater to the air in the Pacific and Indian Oceans at 9.0-8100 (median: 1600) ng/m2/d and 290-2000 (median: 1300) ng/m2/d, respectively. In the Southern Ocean, the net flow of PAHs was from air to seawater with a flux of -1000-450 (median: -82) ng/m2/d. Source apportionment from two different models suggested that the largest contribution to total PAHs was from petrogenic sources (44-57%), followed by coal/wood combustion (30-31%), fossil fuel combustion (15%), and engine combustion emissions (2.8-9.5%). Higher contributions from petrogenic sources were found at sites close to coastal regions. Both coal/wood combustion and petrogenic sources are responsible for the PAH concentrations detected in the Indian and Southern Oceans.

17.
Artigo em Inglês | MEDLINE | ID: mdl-34831577

RESUMO

Using data from the 2019 CIRP Freshman Survey and the Your First College Year (YFCY) from the Higher Education Research Institute at UCLA, this study explores the differences between the characteristics and behaviors of the first-year students with autism spectrum disorders (17) and those of students with learning disabilities (102). The findings indicate that the characteristics of these two groups of first-year college students were similar except for gender, ethnicity, first college generation, and parents' income. Compared with first-year college students with LD, students with ASD were less likely to engage in risk-taking behaviors, use health services and the writing center.

18.
Nano Lett ; 21(20): 8650-8656, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34609149

RESUMO

A perovskite microlaser is potentially valuable for integrated photonics due to its excellent properties. The artificial microlasers were mostly made on polycrystalline films. Though a perovskite single crystal has significantly improved properties in comparison with its polycrystalline counterpart, an artificial microlaser based on single-crystal perovskite has been much less explored due to the difficulty in producing an ultrathin-single-crystal (UTSC) film. Here we show a device processing based on a perovskite UTSC film, confirming the high performance of the UTSC device with a quality factor of 1250. The single-crystal device shows 4.5 times the quality factor and 8 times the radiation intensity in comparison with its polycrystalline counterpart. The experiment first proved that hybrid perovskite microlasers with a subwavelength fine structure can be processed by focused ion beams (FIB). In addition, a wavelength-tunable distributed feedback (DFB) laser is demonstrated, with a tuning range of ∼4.6 nm. The research provides an easily applicable approach for perovskite photonic devices with excellent performance.

19.
Natl Sci Rev ; 8(3): nwaa289, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34691600

RESUMO

Surface and interface play critical roles in energy storage devices, calling for operando characterization techniques to probe the electrified surfaces/interfaces. In this work, surface science methodology, including electron spectroscopy and scanning probe microscopy, has been successfully applied to visualize electrochemical processes at operating electrode surfaces in an Al/graphite model battery. Intercalation of anions together with cations is directly observed in the surface region of a graphite electrode with tens of nanometers thickness, the concentration of which is one order higher than that in bulk. An intercalation pseudocapacitance mechanism and a double specific capacity in the electrode surface region are expected based on the super-dense intercalants and anion/cation co-intercalation, which are in sharp contrast to the battery-like mechanism in the electrode bulk. The distinct electrochemical mechanism at the electrode surface is verified by performance tests of real battery devices, showing that a surface-dominant, nanometer-thick graphite cathode outperforms a bulk-dominant, micrometer-thick graphite cathode. Our findings highlight the important surface effect of working electrodes in charge storage systems.

20.
J Transl Med ; 19(1): 429, 2021 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-34656128

RESUMO

BACKGROUND: Colorectal cancer is one of the most common malignancy in the world. It has been reported that cancer stem cells (CSCs) serve as the primary drivers of tumorigenesis and tumor progression. There is an urgent need to explore novel molecules that regulate CSCs or their signatures. Increasing evidence has shown that miRNAs are involved in tumorigenesis and progression. Here, we aim to explore the regulatory effect and mechanism of miR-3065-3p on the stemness of colorectal cancer. METHODS: The expression of miR-3065-3p in colorectal cancer and the association of miR-3065-3p expression with prognosis of patients with colorectal cancer were analyzed using TCGA dataset or clinical cases. Gain or loss of function in different models, including colorectal cancer cell lines and orthotopic xenograft or liver metastatic mouse model, were used to investigate the effects of miR-3065-3p on colorectal cancer stemness and metastasis in vitro and in vivo. Cancer stemness was analyzed by detecting the ability of migration and invasion, NANOG, OCT4, and SOX2 expression, ALDH activity and sphere formation. In addition, the interaction of miR-3065-3p and cytokine receptor-like factor 1 (CRLF1) was analyzed theoretically and identified by the luciferase reporter assay. Moreover, the correlation between CRLF1 expression and miR-3065-3p was analyzed in colorectal cancer tissues. Finally, the effect of CRLF1 on the stemness and metastasis of colorectal cancer in vitro and in vivo was assessed. RESULTS: In this report, we found that miR-3065-3p was overexpressed in colorectal cancer and that its high expression was associated with poor prognosis of patients with colorectal cancer. miR-3065-3p promotes the stemness and metastasis of colorectal cancer. Furthermore, CRLF1 was the downstream target of miR-3065-3p and inhibited the stemness of colorectal cancer. In addition, CRLF1 expression was negatively correlated with miR-3065-3p in colorectal cancer tissues. And, CRLF1 mediated the effects of miR-3065-3p on promoting stemness of colorectal cancer cells. CONCLUSION: Our data suggest that miR-3065-3p promoted the stemness and metastasis of colorectal cancer by targeting CRLF1. miR-3065-3p might serve as a promising prognostic marker as well as a therapeutic target for colorectal cancer.


Assuntos
Neoplasias Colorretais , MicroRNAs , Animais , Linhagem Celular Tumoral , Neoplasias Colorretais/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , MicroRNAs/genética , Metástase Neoplásica , Receptores de Citocinas
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