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1.
Neural Regen Res ; 18(3): 683-688, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36018194

RESUMO

Percutaneous electrical nerve stimulation of an injured nerve can promote and accelerate peripheral nerve regeneration and improve function. When performing acupuncture and moxibustion, locating the injured nerve using ultrasound before percutaneous nerve stimulation can help prevent further injury to an already injured nerve. However, stimulation parameters have not been standardized. In this study, we constructed a multi-layer human forearm model using finite element modeling. Taking current density and activated function as optimization indicators, the optimal percutaneous nerve stimulation parameters were established. The optimal parameters were parallel placement located 3 cm apart with the injury site at the midpoint between the needles. To validate the efficacy of this regimen, we performed a randomized controlled trial in 23 patients with median nerve transection who underwent neurorrhaphy. Patients who received conventional rehabilitation combined with percutaneous electrical nerve stimulation experienced greater improvement in sensory function, motor function, and grip strength than those who received conventional rehabilitation combined with transcutaneous electrical nerve stimulation. These findings suggest that the percutaneous electrical nerve stimulation regimen established in this study can improve global median nerve function in patients with median nerve transection.

2.
Clin. transl. oncol. (Print) ; 24(10): 1833–1843, octubre 2022.
Artigo em Inglês | IBECS | ID: ibc-207940

RESUMO

Chimeric antigen receptor (CAR) T cell therapy is a novel therapeutic approach that uses gene editing techniques and lentiviral transduction to engineer T cells so that they can effectively kill tumors. However, CAR T cell therapy still has some drawbacks: many patients who received CAR T cell therapy and achieve remission, still had tumor relapse and treatment resistance, which may be due to tumor immune escape and CAR T cell dysfunction. To overcome tumor relapse, more researches are being done to optimize CAR T cell therapy to make it more precise and personalized, including screening for more specific tumor antigens, developing novel CAR T cells, and combinatorial treatment approaches. In this review, we will discuss the mechanisms as well as the progress of research on overcoming plans. (AU)


Assuntos
Humanos , Antígenos , Neoplasias , Imunoterapia Adotiva , Linfócitos T , Microambiente Tumoral
3.
J Fungi (Basel) ; 8(9)2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36135630

RESUMO

Cucurbitariaceae has a high biodiversity worldwide on various hosts and is distributed in tropical and temperate regions. Woody litters collected in Changchun, Jilin Province, China, revealed a distinct collection of fungi in the family Cucurbitariaceae based on morphological and molecular data. Phylogenetic analyses of the concatenated matrix of the internal transcribed spacer (ITS) region, the large subunit (LSU) of ribosomal DNA, the RNA polymerase II subunit (rpb2), the translation elongation factor 1-alpha (tef1-α) and ß-tubulin (ß-tub) genes indicated that the isolates represent Allocucurbitaria and Parafenestella species based on maximum likelihood (ML), maximum parsimony (MP) and Bayesian analysis (BPP). We report four novel species: Allocucurbitaria mori, Parafenestella changchunensis, P. ulmi and P. ulmicola. The importance of five DNA markers for species-level identification in Cucurbitariaceae was determined by Assemble Species by Automatic Partitioning (ASAP) analyses. The protein-coding gene ß-tub is determined to be the best marker for species level identification in Cucurbitariaceae.

4.
J Ethnopharmacol ; 300: 115675, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36075275

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Rheum palmatum L. (RP) and Coptis chinensis Franch. (CC), frequently used as herbal pair (HP) in clinical practicing of traditional Chinese medicine, exerted predominate efficacies in colitis treatment. However, the mechanism of their synergism lacks scientific explanation. AIM OF THE STUDY: By integrating network pharmacology and DSS-induced colitis model, the anti-colitis effects and synergistic molecular mechanisms of RP-CC combination was determined. MATERIALS AND METHODS: In vivo study, mice were divided into control, model, RP, CC and RP-CC (low, middle, high) groups, 2.5% DSS was administrated to induce colitis for consecutive 7 days, subsequently, the therapeutic effects were evaluated from body weight changes, disease activity index (DAI), and pathological conditions. After determining the shared and exclusive targets of RP and CC, respectively by network pharmacology, CETSA, WB, and qPCR were utilized to verify the action modes of RP and CC on specific targets. RESULTS: Compared to RP or CC used alone, RP-CC combination can significantly protect colon tissues from inflammatory damage in a dose-dependent manner via remarkably alleviating DAI and colon shortening. Network pharmacological analysis suggested that AKT1 would be the core target for RP-CC synergism since these two herbs could simultaneously but non-competitively bind to AKT1 at different sits. Furthermore, RP and CC could also influencing HIF and MAPK pathways, respectively, these additional actions attribute to more optimizing effectiveness towards colitis. CONCLUSION: In contrast to the mild therapeutic effects of RP or CC individually, RP-CC herb pair could exert strong and synergistic effects in treatment of colitis via non-competitive binding to AKT1 simultaneously, as well as exclusively influencing MAPK and HIF pathways. Our study not only provides the evidence for understanding the combined effect of RP and CC, but also brings up a new strategy and suggestive thoughts for the rationality of HP-based TCM formula.

5.
Sci Total Environ ; 854: 158638, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36089010

RESUMO

The atmospheric urban heat island (AUHI) effect, traditionally measured by in-situ sensors mounted on fixed meteorological stations, has been extensively studied by different and imperfect methods. However, facts and uncertainties of the AUHI estimates revealed by the different methods are not well understood at a large scale. Here we examined the spatial-temporal variations of the AUHI effects from multiple perspectives in China's 86 large cities as revealed by national-level meteorological observations at 2-m height from 1981 to 2017. We find relatively consistent patterns of larger urban heating effects in daily minimum temperature, winter, and Northeast China than their counterparts in terms of multiyear mean intensity (AUHII), long-term trend (△AUHII), and contribution to local warming (according to the CTRUMR "urban minus rural" and CTROMR "observation minus reanalysis" methods). Concurrently, a cooling impact or a reduction in the heating effect has been observed in some cities randomly, especially in daily maximum temperature. On average across cities, the AUHII, △AUHI, CTRUMR, and CTROMR for the daily mean temperature amount to 0.33 °C, 0.013 °C 10a-1, 53 %, and 23 % at an annual mean time scale, respectively. Nevertheless, the poor representativeness of weather stations, discrepancies among the quantification methods, nonlinearity of the long-term tendencies, and coupling effects with rural crop land use activities lead to large uncertainties of the AUHI estimates. Our results emphasize the limitations of national-level meteorological stations in characterizing AUHI in China and suggest that the urban heat island remains a "well described but rather poorly understood" phenomenon warranting further investigation by a combined uses of multiple techniques like high-density sensor networks, remote sensing techniques, and high-resolution numerical models.

6.
Exp Neurol ; 358: 114228, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36108713

RESUMO

BACKGROUND AND PURPOSE: Enhancing phagocytosis can facilitate the removal of inflammatory molecules, limit the toxicity of dead cells and debris, and promote recovery after brain injury. In this study, we aimed to explore the role of bexarotene (Bex), a retinoid X receptor (RXR) agonist, in promoting astrocyte phagocytosis and neurobehavioral recovery after subarachnoid hemorrhage (SAH). METHODS: Mice SAH model was induced by pre-chiasmatic injection of blood. Modified Garcia score, novel object recognition, rotarod test, and Morris water maze were performed to assess neurological function. Immunofluorescence and electron microscopy were used to evaluate astrocyte phagocytosis in vivo. In addition, ABCA1/MEGF10&GULP1, the primary astrocyte phagocytosis pathway, were stimulated by Bex or suppressed by HX531 (a RXR antagonist) to evaluate their impacts on astrocyte phagocytosis and neurological recovery. RESULTS: Astrocytes phagocytosis of blood components were observed in mice after SAH induction, which is further increased by Bex treatment. Bex dramatically attenuated neuroinflammation, reduced brain edema, improved early neurological performance and promoted neurocognitive recovery. Meanwhile, Bex decreased neurotoxic reactive astrocytes and preserved neurogenesis after SAH. Bex increased the expression of astrocyte phagocytosis-related proteins ABCA1, MEGF10, and GULP1. Bex also increased the lysosomal processing of engulfed blood components in astrocytes. Moreover, Bex significantly promoted astrocytes to phagocytize debris in vitro by increasing the expression of ABCA1, MEGF10 and GULP1, while HX531 inhibited astrocyte phagocytosis and decreased these protein levels. CONCLUSIONS: Bex enhanced astrocyte phagocytosis through the ABCA1-mediated pathways, and promoted neurobehavior recovery in mice after SAH induction.

7.
Environ Res ; 215(Pt 2): 114289, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36116493

RESUMO

Chronic kidney disease (CKD) has been a global public health problem with many adverse outcomes, but data are lacking regarding the relationship between air pollutants and risk of renal progression in patients with CKD. This study was to investigate whether 1-year average exposure to ambient air pollutants -CO, NO, NO2, SO2, O3, PM2.5, and PM10-is related to renal function deterioration among patients with CKD. A total of 5301 CKD patients were included in this study between October 2008 and February 2016. To estimate each patient's exposure to ambient air pollution, we used the 24-h ambient air pollution concentration monitoring data collected one year prior to renal progression or their last renal function assessment. Renal progression was considered when estimated glomerular filtration rate (eGFR) decreased more than 25% from the baseline eGFR. Cox proportional hazard regression was performed to calculate hazard ratios (HRs). Among 5301 patients with CKD, 1813 (34.20%) developed renal progression during the 30.48 ± 14.99-month follow-up. Patients with the highest quartile exposure to CO [HR = 1.53 (95% CI: 1.24, 1.88)], NO [HR = 1.38 (95% CI: 1.11, 1.71)], NO2 [HR = 1.63 (95% CI: 1.36, 1.97)], SO2 [HR = 2.27 (95% CI: 1.83, 2.82)], PM2.5 [HR = 7.58 (95% CI: 5.97, 9.62)], and PM10 [HR = 3.68 (95% CI: 2.84, 4.78)] had a significantly higher risk of renal progression than those with the lowest quartile exposure. In the multipollutant model, the analyses yielded to similar results. These results reinforce the importance of measures to mitigate air pollution and strategies to prevent worsening of kidney function in patients with CKD. One-year high exposure to ambient CO, NO, NO2, SO2, PM2.5, and PM10 is significantly associated with deteriorated kidney function in patients with CKD among Taiwanese adults.

8.
Bioresour Technol ; : 127992, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36150424

RESUMO

The anaerobic ammonia oxidation (anammox) process is a promising biological nitrogen removal technology. However, owing to the sensitivity and slow cell growth of anammox bacteria, long startup time and low nitrogen removal rate (NRR) are still limiting factors of practical applications of anammox process. Moreover, nitrogen removal efficiency (NRE) is often lower than 88%. This review summarizes the most common methods for improving NRR by increasing microorganism concentration, and modifying reactor configuration. Recent integrated anammox-based systems were evaluated, including hydroxyapatite (HAP)-enhanced anammox for a high NRR of over 2 kg N/m3/d, partial denitrification/anammox (PDA) process, and simultaneous partial nitrification, anammox, and denitrification process for a high NRE of up to 100%. After discussing the challenges for the application of these systems critically, a combined system of anaerobic digestion, HAP-enhanced one-stage partial nitritation/anammox and PDA is proposed in order to achieve a high NRR, high NRE, and phosphorus removal simultaneously.

9.
Clin Chim Acta ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36150521

RESUMO

Chronic inflammation caused by immune cells and their mediators is a characteristic of atherosclerosis. Interleukin-38 (IL-38), a member of the IL-1 family, exerts multiple anti-inflammatory effects via specific ligand-receptor interactions. Upon recognizing a specific receptor, IL-38 restrains mitogen-activated protein kinase (MAPK), nuclear factor kappa B (NK-κB), or other inflammation-related signaling pathways in inflammatory disease. Further research has shown that IL-38 also displays anti-atherosclerotic effects and reduces the occurrence and risk of cardiovascular events. On the one hand, IL-38 can regulate innate and adaptive immunity to inhibit inflammation, reduce pathological neovascularization, and inhibit apoptosis. On the other hand, it can curb obesity, reduce hyperlipidemia, and restrain insulin resistance to reduce cardiovascular disease risk. Therefore, this article expounds on the vital function of IL-38 in the development of atherosclerosis to provide a theoretical basis for further in-depth studies of IL-38 and insights on the prophylaxis and treatment of atherosclerosis.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36151061

RESUMO

Light-responsive adsorbents capture significant attention due to their tailorable performance upon light irradiation. The modulation of such adsorbents is mainly based on weak (physical) interactions caused by steric hindrance while tuning strong interaction with target adsorbates is scarce. Here we report smart π-complexation adsorbents, which can adjust the π-complexation of active sites via light irradiation. A typical metal-organic framework, MIL-101-NH 2 , was decorated with azobenzene motifs, and Cu + as π-complexation active sites were introduced subsequently. The reversible light-induced isomerization of azobenzene regulates the surface electrostatic potentials around Cu + from -0.038 to 0.008 eV, causing shielding and exposure effects. The alteration of CO uptake is achieved up to 54% via changing light, while that on MIL-101-NH 2 is negligible. This study provides a clue for designing target-specific smart materials to meet the practical stimuli-responsive adsorption demands.

11.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(9): 1042-1046, 2022.
Artigo em Chinês | MEDLINE | ID: mdl-36111724

RESUMO

OBJECTIVES: To study the correlation between neck circumference and body mass index and the value of neck circumference in identifying overweight and obesity in preschool children. METHODS: The stratified cluster sampling method was used to recruit 3 719 children under 7 years from 10 kindergartens in Urumqi, China. General data were collected, and physical measurements were performed. A Pearson correlation analysis was used to evaluate the correlation between neck circumference and body mass index. The receiver operating characteristic (ROC) curve was used to assess the accuracy of neck circumference in identifying overweight/obesity. The Kappa consistency test was used to assess the consistency of neck circumference and body mass index in identifying overweight/obesity. RESULTS: There was a positive correlation between neck circumference and body mass index in boys and girls of all ages (r≥0.50, P<0.001). According to body mass index as the criteria for overweight/obesity, the children were divided into an overweight/obesity group and a non-overweight/obesity group, and the analysis showed that the overweight/obesity group had a significantly larger neck circumference than the non-overweight/obesity group (P<0.001). The ROC curve analysis showed that neck circumference had an area under the ROC curve of >0.7 in identifying overweight/obesity for boys and girls. The Kappa consistency test showed that the neck circumference and body mass index had a Kappa value of >0.40 in identifying overweight/obesity in boys and girls of all ages. CONCLUSIONS: Neck circumference is positively correlated to body mass index, and neck circumference can be used to identify overweight/obesity in preschool children.


Assuntos
Obesidade , Sobrepeso , Índice de Massa Corporal , Pré-Escolar , China , Feminino , Humanos , Masculino
12.
J Sleep Res ; : e13723, 2022 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-36116773

RESUMO

This study aims to investigate the temporal links between physical activity, sleep and affective wellbeing in young adults. In particular, the aim was to examine whether physical activity is associated with sleep indicators in subsequent nights and, in turn, whether sleep was associated with improved affective wellbeing the next morning. Relatedly, moderation by baseline levels of depressive symptoms, sleep quality, habitual physical activity and gender was analysed. One-hundred and forty-seven individuals (85.7% female) aged 18-25 years old participated in an experience sampling study over 14 consecutive days. Participants received seven prompts per day, and answered questions about their physical activity and affective states. Every morning, participants also reported their sleep. Physical activity throughout the day was not related to sleep during the following night or to affective wellbeing the next morning. An exception to that pattern was that physical activity before 14:00 hours was associated with longer subsequent sleep duration. Better subjective sleep quality predicted affective wellbeing the next morning. Associations of physical activity, sleep and affective wellbeing were not moderated by baseline depressive symptoms, sleep quality or habitual physical activity. However, investigation of gender as a moderator revealed that moderate physical activity was associated with better subsequent sleep quality for males, but not for females. Overall, we found that physical activity is associated with better subsequent sleep for males, but not for females. Also, our study provides further evidence that better sleep quality is associated with the next morning's affective wellbeing.

13.
J Crit Care ; 72: 154138, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36084378

RESUMO

BACKGROUND: We systematically assessed whether a digital polymerase chain reaction (PCR) could detect pathogenic microorganisms in patients with sepsis early and accurately. METHODS: We searched the Cochrane Library, MEDLINE, Embase, CNKI, CBM, and Wanfang Data databases for eligible studies to compare the detection of pathogenic microorganisms in blood samples by digital PCR with the gold standard. The Quality Assessment of Diagnostic Accuracy Studies 2 was used to evaluate bias risk, and a random-effects meta-analysis approach was used for sensitivity and specificity calculations. RESULTS: Among the eight articles, there were eight identified studies with a total of 1278 subjects. The pooled sensitivity of digital PCR was 94% (95% confidence interval [CI], 85%-98%), the specificity was 87% (95% CI, 76%-94%), the positive likelihood ratio was 7.3 (95% CI, 3.8-14.2), the negative likelihood ratio was 0.07 (95% CI, 0.03-0.17), the positive predictive value was 84.7%, the negative predictive value was 89.2%, the diagnostic odds ratio was 105 (95% CI, 37-303), and the area under the receiver operating characteristic curve was 0.97 (95% CI, 0.95-1.00). Digital PCR can shorten the detection time of pathogenic microorganisms in patients with sepsis. CONCLUSIONS: Digital PCR can detect pathogenic microorganisms in patients with sepsis earlier than blood culture. Therefore, digital PCR can be used as a potential strategy for the detection of pathogenic microorganisms in patients with sepsis.

14.
Sci Total Environ ; 852: 158586, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36075441

RESUMO

The anaerobic membrane bioreactor (AnMBR) has gained huge attention as a municipal wastewater (MWW) treatment process that combined high organics removal, a low sludge yield and bioenergy recovery. In this study, a 20 L AnMBR was set up and operated steadily for 70 days in temperate conditions with an HRT of 6 h and a flux of 12 LMH for the treatment of real MWW, focusing on the behavior of the major elements (C, N, P and S) from an elemental balance perspective. The results showed that the AnMBR achieved more than 85 % COD removal, a low sludge yield (0.081 gVSS/gCODremoved) and high methane production (0.31 L-CH4/gCODremoved) close to the theoretical value. The elemental flow analysis revealed that the AnMBR converted 77 % of the influent COD to methane (57 % gaseous and 20 % dissolved) and 6 % of the COD for sludge production. In addition, the AnMBR converted 34 % of the total carbon to energy-generated carbon, and only 3 % was in the form of CO2 in the biogas for further upgradation, which was in line with the concept of carbon neutrality. Since little nitrogen or phosphorus were removed, the permeate was nutrient-rich and further treatment to recover the nutrients would be required. This study illustrates the superior performance of the AnMBR for MWW treatment with a microscopic view of elemental behavior and provides a reference for implementing the mainstream AnMBR process in carbon-neutral wastewater treatment plants.

15.
Nat Commun ; 13(1): 5460, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36115857

RESUMO

Photocatalytic water splitting is attracting considerable interest because it enables the conversion of solar energy into hydrogen for use as a zero-emission fuel or chemical feedstock. Herein, we present a universal approach for inserting hydrophilic non-conjugated segments into the main-chain of conjugated polymers to produce a series of discontinuously conjugated polymer photocatalysts. Water can effectively be brought into the interior through these hydrophilic non-conjugated segments, resulting in effective water/polymer interfaces inside the bulk discontinuously conjugated polymers in both thin-film and solution. Discontinuously conjugated polymer with 10 mol% hexaethylene glycol-based hydrophilic segments achieves an apparent quantum yield of 17.82% under 460 nm monochromatic light irradiation in solution and a hydrogen evolution rate of 16.8 mmol m-2 h-1 in thin-film. Molecular dynamics simulations show a trend similar to that in experiments, corroborating that main-chain engineering increases the possibility of a water/polymer interaction. By introducing non-conjugated hydrophilic segments, the effective conjugation length is not altered, allowing discontinuously conjugated polymers to remain efficient photocatalysis.

16.
Int J Qual Stud Health Well-being ; 17(1): 2122138, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36120892

RESUMO

PURPOSE: Problem solving has been defined as "a goal-directed sequence of cognitive and affective operations as well as behavioural responses to adapting to internal or external demands or challenges. Studies have shown that some nurses lack rational thinking and decision-making ability to identify patients' health problems and make clinical judgements, and have poor cognition and response to some clinical problems, easy to fall into problem-solving dilemma. This study aimed to understand the influencing factors of clinical nurses' problem solving dilemma, to provide a basis for developing training strategies and improving the ability of clinical nurses in problem solving. METHODS: A qualitative research was conducted using in-depth interviews from August 2020 to December 2020. A total of 14 participants from a tertiary hospital in Shanghai, China were recruited through purposive sampling combined with a maximum variation strategy. Data were analysed with the conventional content analysis method. RESULTS: Three themes and seven subthemes were extracted: nurse's own factors (differences in knowledge structure and thinking, differences in professional values, poor strain capacity); improper nursing management (low sense of organizational support, contradiction between large workload and insufficient manpower allocation); patient factors (the concept of emphasizing medicine and neglecting to nurse, individual differences of patients). CONCLUSION: The influencing factors of clinical nurses' problem-solving dilemma are diverse. Hospital managers and nursing educators should pay attention to the problem-solving of clinical nurses, carry out a series of training and counselling of nurses by using the method of situational simulation, optimize the nursing management mode, learn to use new media technology to improve the credibility of nurses to provide guarantee for effective problem-solving of clinical nurses.


Assuntos
Enfermeiras e Enfermeiros , Resolução de Problemas , China , Humanos , Pesquisa Qualitativa
17.
Artigo em Inglês | MEDLINE | ID: mdl-36123501

RESUMO

Two of the most important concepts in working memory that differentiate many theories are the role of attention and similarity between items. Investigators have debated whether there is a central, general resource of attention, and whether interference between items depends mostly on their modality and type of code (i.e., verbal/acoustic versus visual/spatial coding) or upon multiple dimensions of feature similarity even within a modality. Here, we examine results from three experiments in which the features of items to be remembered differed for visual objects in color or orientation, or for acoustic objects in noise duration or tone pitch. There were one or two of these sets on a trial and, when there were two sets, the similarity between their features varied: there were sets in different modalities, sets with different feature types within a modality, or sets of the same feature type. One-set trials consistently produced superior performance. For two-set trials, dissimilarity of the sets mattered only when both sets had to be attended, compared with attention to only one set. Feature differences within a modality mattered at least as much as between-modality differences. The findings conflict with what would be expected if modality were the sole organizing principle and support a working memory model in which a capacity-limited attention is constrained by the feature similarity of task-relevant items.

19.
Comput Struct Biotechnol J ; 20: 5040-5044, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36097552

RESUMO

The binding of SARS-CoV-2 nucleocapsid (N) protein to both the 5'- and 3'-ends of genomic RNA has different implications arising from its binding to the central region during virion assembly. However, the mechanism underlying selective binding remains unknown. Herein, we performed the high-throughput RNA-SELEX (HTR-SELEX) to determine the RNA-binding specificity of the N proteins of various SARS-CoV-2 variants as well as other ß-coronaviruses and showed that N proteins could bind two unrelated sequences, both of which were highly conserved across all variants and species. Interestingly, both sequences are virtually absent from the human transcriptome; however, they exhibit a highly enriched, mutually complementary distribution in the coronavirus genome, highlighting their varied functions in genome packaging. Our results provide mechanistic insights into viral genome packaging, thereby increasing the feasibility of developing drugs with broad-spectrum anti-coronavirus activity by targeting RNA binding by N proteins.

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