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1.
J Nanosci Nanotechnol ; 20(3): 1697-1703, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492332

RESUMO

TiO2 doped layered zirconium phosphates were prepared by the hydrofluoric acid (HF) method and its photocatalytic performance was investigated in this study. Through the introduction of octylamine which acts as the intercalation and exfoliation reagent in the process, TiO2 could be uniformly generated and dispersed on the zirconium phosphate matrix through tetrabutyl titanate hydrolysis and calcination. The nano-scale TiO2 was obtained by applying the appropriate ratio of tetrabutyl titanate and layered zirconium phosphate in reaction. XRD, N2-sorption, FT-IR, UV-vis, SEM and TEM were used to characterize the structure and phtocatalytic properties of the samples. The photocatalytic performance of synthesized nano-scale TiO2 doped zirconium phosphates was studied by degradation of Rhodamine B (RhB). It is found that the scavenging rate of RhB could be up to 65% within 90 min under the visible light irradiation due to the relatively large active surface area and compact size of TiO2. This study highlights the potential application of TiO2 doped layered zirconium phosphate as a novel photocatalyst in photocatalytic degradation of organic pollutants.

2.
J Obstet Gynaecol ; : 1-7, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478415

RESUMO

This study aimed to explore the effects of low-concentration (0.6 ng/mL) granulocyte-macrophage colony-stimulating factor (GM-CSF) supplementation on human embryo quality and pregnancy outcomes in patients with fresh transfer cycles. The data were retrospectively collected from 719 hyperstimulation cycles of 631 patients divided into two groups: GM-CSF supplementation (0.6 ng/mL, n = 399) and control (n = 320). The embryo quality and pregnancy outcomes were compared. GM-CSF addition significantly increased the available embryo rate (52.0 vs. 48.1%, p < .05). In patients >38 years, it significantly enhanced cleavage (99.4 vs. 97.8%, p < .05) and blastocyst formation (45.7 vs. 34.9%, p < .05) rates but not pregnancy outcomes, including clinical pregnancy (power = 0.160) and implantation (power = 0.204) rates. The lack of a statistically significant difference could be related to low study power. These results suggest that low-concentration GM-CSF addition contributes to embryo quality improvement, especially in patients >38 years. IMPACT STATEMENT What is already known on this subject? Granulocyte-macrophage colony-stimulating factor (GM-CSF) has a beneficial effect on the development of human embryos in assisted reproductive technology. What do the results of this study add? Adding 0.6 ng/mL GM-CSF significantly increased the available embryo rate. In patients over 38 years of age, it statistically significantly enhanced the cleavage rate (99.4 vs. 97.8%, p < .05) and blastocyst formation rate (45.7 vs. 34.9%, p < .05). What are the implications of these findings for clinical practice and/or further research? GM-CSF benefits embryos with poorer development potential and a randomised clinical trial with a larger sample size should be performed.

3.
J Exp Bot ; 2019 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-31494666

RESUMO

Excessive cadmium (Cd) accumulation in rice poses a potential threat to human health. Rice varieties vary in their Cd content, which depend mainly on root-to-shoot Cd translocation. However, the cultivars accumulating high Cd in the natural population have not been completely investigated. In this study, we analyzed the variation in Cd accumulation in a diverse panel of 529 rice cultivars. We observed that only a small proportion (11 of 529) showed extremely high root-to-shoot Cd transfer rates, and seven of them were caused by two known OsHMA3 alleles. Using quantitative trait loci (QTL) mapping, we identified a new OsHMA3 allele that was associated with high Cd accumulation in three of the remaining cultivars. Using heterologous expression in yeast and comparative analysis among different rice cultivars, we observed that this new allele was weak at both transcriptional and protein levels compared to the functional OsHMA3 genotypes. The weak Cd transport activity was further demonstrated to be caused by Gly to Arg substitution at the 512th position. Our study comprehensively analyzed the variation in root-to-shoot Cd translocation rates in cultivated rice and identified a new OsHMA3 allele that caused high Cd accumulation in few rice cultivars.

4.
Environ Sci Technol ; 2019 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-31496231

RESUMO

Psychiatric pharmaceuticals are widely distributed in the aquatic environment and have attracted recent attention due to their potential for environmental effects. A robust and reliable in situ passive sampling approach, the diffusive gradients in thin-films (DGT) technique, is developed here to measure 14 psychiatric pharmaceuticals. A new binding material, mixed-mode cation exchange resin (Poly-Sery MCX, 40µm, CNW, Germany), was used for the first time in DGT and compared to XAD and HLB. Reliable elution efficiencies of the pharmaceuticals from the binding gels were obtained in methanol/ammonia and diffusion coefficients for all the compounds were determined. The influence of diffusive layer thickness (0.515-2.015 mm), deployment time (3-168 h), and important environmental conditions - pH (3.02-9.45), ionic strength (0.0001-0.5 M) and dissolved organic matter (0-20 mg L-1) - were evaluated. The capacity of XAD, HLB and MCX gels for binding all the test pharmaceuticals was ~335 µg per disc, meaning that DGT could theoretically be deployed for over 30 months, if there are no competitive effects or confounding factors. The uptake kinetics of psychiatric pharmaceuticals onto MCX gel were much faster than to XAD and HLB gels in the first hour. DGT measured concentrations of test pharmaceuticals at two sample points in a river (over 6 days) were comparable to those obtained by grab sampling. This study demonstrates the accuracy and reliability of DGT for measuring psychiatric pharmaceuticals across the wide range of freshwater conditions found in the natural environment.

5.
Biotechnol J ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31487423

RESUMO

Scyllo-Inositol (SI), a stereoisomer of inositol, has been regarded as a promising therapeutic agent for Alzheimer's disease. Here, we presented an in vitro cofactor-balance biotransformation for the production of SI from myo-inositol (MI) by thermophilic myo-inositol 2-dehydrogenase (IDH) and scyllo-inositol 2-dehydrogenase (SIDH). These two enzymes (i.e., IDH and SIDH from Geobacillus kaustophilus) were co-expressed in Escherichia coli BL21(DE3), and E. coli cells containing the two enzymes was permeabilized by heat treatment as whole-cell catalysts to convert MI to SI. After condition optimizations about permeabilized temperature, reaction temperature and initial MI concentration, about 82 g/L of SI was produced from 250 g/L of MI within 24 h without any cofactor supplementation. This final titer of SI produced is the highest to our limited knowledge. This study provided a promising method for the large-scale industrial production of SI. This article is protected by copyright. All rights reserved.

6.
Plant Biotechnol J ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31487424

RESUMO

Networks of transcription factors regulate diverse physiological processes in plants to ensure that plants respond to abiotic stresses rapidly and efficiently. In this study, expression of two DREB/CBF genes, TaDREB3 and TaCBF5L was modulated in transgenic wheat and barley, by using stress-responsive promoters HDZI-3 and HDZI-4. The promoters were derived from the durum wheat genes encoding the γ-clade TFs of the HD-Zip class I subfamily. The activities of tested promoters were induced by drought and cold in leaves of both transgenic species. Differences in sensitivity of promoters to drought strength were dependent on drought tolerance levels of cultivars used for generation of transgenic lines. Expression of the DREB/CBF genes under both promoters improved drought and frost tolerance of transgenic barley, and frost tolerance of transgenic wheat seedlings. Expression levels of the putative TaCBF5L downstream genes in leaves of transgenic wheat seedlings were upregulated under severe drought, and up- or down-regulated under frost, compared to those of control seedlings. The application of TaCBF5L driven by the HDZI-4 promoter led to the significant increase of the grain yield of transgenic wheat, compared to that of the control wild-type plants, when severe drought was applied during flowering; although no yield improvements were observed when plants grew under well-watered conditions or moderate drought. Our findings suggest that the studied HDZI promoters combined with the DREB/CBF factors could be used in transgenic cereal plants for improvement of abiotic stress tolerance, and the reduction of negative influence of transgenes on plant development and grain yields. This article is protected by copyright. All rights reserved.

7.
J Food Biochem ; 43(9): e12976, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31489668

RESUMO

Bromelain has wide applications in different industries, such as food, textile, and medicine. Traditional approaches for bromelain separation and purification from solution still have many problems, including unsatisfactory binding efficiency, time-consuming operation, and costly equipment. In the present study, a new type of dendritic polymer-based magnetic carrier (GO@Fe3 O4 @PEI-Cu2+ ) was first prepared for bromelain separation and purification in solution. The histidine existing in bromelain could bind to Cu2+ cations adsorbed on the surface of the magnetic carrier, and the magnetic carrier showed excellent performance for bromelain separation and purification in solution, with the adsorption capacity up to 357 mg/g. The magnetic carrier also exhibited excellent property in the aspect of recyclability. It was found that the magnetic carrier also presented desirable performance for the separation and purification of bromelain from the crude extract of pineapple peel, and the bromelain structure remained intact before and after elution process. PRACTICAL APPLICATIONS: Considering many advantages of bromelain in the applications of pharmaceutical and food industries, this study is aimed at presenting a novel magnetic carrier with high stability and fabulous performance for bromelain separation and purification in solution and achieving the practical application that the magnetic carrier can efficiently separate bromelain from the crude extract of pineapple peel.

8.
Am J Hypertens ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31504137

RESUMO

BACKGROUND: While sex differences characterize susceptibility and severity of idiopathic pulmonary arterial hypertension (IPAH), our understanding of the relationship between levels of gonadotropins and sex hormones in fertile women and the disease is limited. We aimed to investigate whether gonadotropin and sex hormone levels in women of reproductive age were associated with risk and mortality of IPAH. METHODS: We did a matched case-control study. Cases were reproductive female patients with idiopathic pulmonary arterial hypertension admitted in Shanghai Pulmonary Hospital (Tongji University School of Medicine, Shanghai, China) during 2008 to 2014. Healthy controls were matched on age and body mass index. We also did a prospective cohort study to assess the effects of hormone levels on mortality in IPAH fertile female patients. RESULTS: 164 cases and 133 controls were included. After adjustment for age and body mass index, the odds ratios of having IPAH for follicle stimulating hormone, testosterone, and progesterone as expressed on natural log scale were 1.51 (95%CI 1.06, 2.16), 0.42 (0.31-0.57), and 0.52(0.43-0.63), respectively. In the cohort study with a median follow up of 77-months, the hazard ratios for dying after adjustment for baseline characteristics and treatments among IPAH patients were 2.01 (95% CI: 1.22-3.30) and 0.78 (95% CI: 0.62-0.98) for follicle stimulating hormone and progesterone in natural log scale, respectively. CONCLUSIONS: In reproductive women with IPAH, high follicle stimulating hormone and low progesterone tended to be associated with high risk of IPAH and mortality among patients.

10.
Sci Total Environ ; 698: 134289, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31514034

RESUMO

Microeukaryotes are the key ecosystem drivers mediating marine productivity, the food web and biogeochemical cycles. The northwestern Pacific Ocean (NWPO), as one of the world's largest oligotrophic regions, remains largely unexplored regarding diversity and biogeography of microeukaryotes. Here, we investigated the community composition and geographical distribution of microeukaryotes collected from the euphotic zone of three different regions in the NWPO using high-throughput sequencing of the 18S rRNA gene and quantified the contributions of environmental factors on the distributions of microeukaryotes. The relative abundance of different group taxa, except for Ciliophora, presented distinct patterns in each region, and Metazoa and Dinoflagellata dominated the community, contributing approximately half of reads abundance. Spatial and environmental factors explained 66.01% of community variation in the NWPO. Temperature was the most important environmental factor significantly correlated with community structure. Bacterial biomass was also significantly correlated with microeukaryotic distribution, especially for Dinoflagellata and Diatomea. Network analysis showed strong correlations between microeukaryotic groups and free-living bacteria and different bacterial taxa were correlated with specific microeukaryotic groups, indicating that their interactions enabled microeukaryotic groups to adapt to diverse environments. This study provides a first glance at the diversity and geographical distribution of microeukaryotes in the NWPO and sheds light on the biotic and abiotic factors in shaping the microeukaryotic community in the ocean.

11.
BMC Pediatr ; 19(1): 305, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477056

RESUMO

BACKGROUND: Congenital bronchopulmonary foregut malformation (CBPFM) is a rare congenital malformation involving both the digestive and respiratory system. Early diagnosis is difficult, and delayed recognition may result in considerable complications. The aim of the study was to identify and analyze the clinical characteristics and radiological features of CBPFMs. METHODS: A systematic review was conducted in accordance with PRISMA guidelines. PubMed, Ovid database, EMBASE were searched for relevant publications to identify all published case-reports of CBPFM since 1992. Data about the demography, clinical presentation, pathology, imaging features, treatment and prognosis were collected. RESULTS: Sixty-one cases were included in our study. Cases were aged from 1 day to 59 years with the majority aged 3 years or younger. The most common type was group III (37.7%), followed by group II (29.5%)group I (27.9%) and group IV (4.9%). The presentations included respiratory distress (32.8%), cough/choking following food intake and other presentations associated respiratory infection. Thirty-eight cases (62.3%) were diagnosed by upper gastrointestinal series (UGI). Misdiagnosis was common. Eight cases (13.1%) of the included cases died. CONCLUSIONS: Early recognition and extensive delineation of the anatomy of CBPFM are important to correct these anomalies successfully. UGI is the first choice to confirm the abnormal bronchus communicating with the esophagus. Resection of abnormal pulmonary tissue, lobe or even unilateral lung is preferred. Reconstruction procedures are feasible in selected patients.

12.
Phytochem Anal ; 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31512326

RESUMO

INTRODUCTION: Polygoni Orientalis Fructus (POF) is a clinically effective Chinese medicine. Raw POF (RPOF) and POF Tostus (POFT) are used separately in clinics. However, incomplete progress has been made on quality control. OBJECTIVE: To establish a comprehensive method for quality assessment of RPOF and POFT and to discriminate these two varieties. METHODOLOGY: High-performance liquid chromatography combined with the diode array detector (HPLC-DAD) methods were developed for fingerprinting and quantitative analysis of seven major compounds in RPOF and POFT, and the main components were determined by HPLC-DAD coupled with Fourier-transform ion cyclotron resonance-mass spectrometry. Chemometric approaches were performed to discriminate RPOF and POFT and to screen discriminatory components. RESULTS: Fingerprints were established and 12 common peaks were identified, cannabisin G and cannabisin E were firstly identified from POF. In quantitative analysis, all analytes showed good regression (R > 0.9996) within test ranges and the recovery of the method was in the range 96.6-104.3%. Fingerprints in conjunction with similarity analysis and hierarchical clustering analysis (HCA) demonstrated the consistent quality of RPOF and showed a clear discrimination between RPOF and POFT. Principal component analysis, partial least-squares discriminant analysis, and heatmap-HCA on quantitative data not only gave a clear differentiation between RPOF and POFT, but they also suggested that quercetin, 3,5,7-trihydroxychromone, and N-trans-feruloyltyramine acted as the main factors responsible for the sample differences. CONCLUSIONS: Chromatographic analysis in combination with chemometric analysis provides a simple and reliable method of comparing and evaluating the qualities of RPOF and POFT.

13.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 33(9): 1108-1115, 2019 Sep 15.
Artigo em Chinês | MEDLINE | ID: mdl-31512451

RESUMO

Objective: To report the preliminary clinical results and analyze the prognostic factors of prosthetic failures with non-cemented modular prosthetic reconstruction after tumor resection in lower extremities. Methods: A clinical data of 150 patients with lower extremity tumors treated with MEGASYSTEM-C non-cemented modular prosthetic reconstruction between October 2011 and September 2016 was retrospectively analyzed. There were 88 males and 62 females, aged from 12 to 81 years, with a median age of 24 years. According to World Health Organization (WHO) classification of bone tumors, 120 cases were primary malignant tumors, 27 cases were intermediate tumors, and 3 cases were metastatic tumors. Among them, 134 cases underwent primary operation and 16 cases underwent reoperation after recurrence. Eighty-seven patients with malignant tumors received chemotherapy before and after operation, and no patient received local radiotherapy during perioperative period. Proximal femur was reconstructed in 32 cases, distal femur in 83 cases, and proximal tibia in 35 cases. The postoperative follow-up time, the results of oncology (survival status and tumor recurrence), and prosthesis failure (prosthesis survival rate, reasons for failure, treatment plan after failure) were recorded. The reason of the prosthesis failure was classified into 5 types according to the classification defined by Henderson et al. Kaplan-Meier survival analysis and Log-Rank test were used to analyze patient and prosthesis survival. Lower extremity function was assessed by using the Musculoskeletal Tumor Society (MSTS) scoring system and MSTS scores were compared for patients with different reconstruction sites. Results: All patients were followed up 5-84 months, the median follow-up time was 39 months. During the follow-up period, there were 116 cases of tumor-free survival, 10 cases of tumor-bearing survival, and 24 died of lung metastasis or multiple metastases. The 3-year and 5-year survival rates of 120 patients with primary malignant tumors were 83.1% and 76.6%. There was no significant difference in survival rate between different reconstruction sites ( P=0.851). Seven cases (4.7%) had local recurrence at 7-21 months after operation. The 3-year and 5-year survival rates of the prosthesis in 150 patients were 94.4% and 92.5%. There was no significant difference in survival rate between different reconstruction sites ( P=0.765). There were 26 failures in 24 patients (16.0%) during the follow-up period. There were 9 cases of type 1 failure, 1 case of type 2 failure, 3 cases of type 3 failure, 5 cases of type 4 failure, and 8 cases of type 5 failure. At last follow-up, 120 of the 126 patients survived without prosthetic failure. Except that the influence of different parts of prosthesis on the incidence of type 4 failure ( P=0.029), the influence of chemotherapy on the incidence of type 5 failure ( P=0.002) were significant, the influence of other types of failure on different reconstructed parts of prosthesis, initial operation, and perioperative chemotherapy had no significant difference ( P>0.05). There were 5 cases of amputation (4 cases of type 5 failure, 1 case of type 4 failure), 3 cases of prosthesis removal (1 case of type 2 failure, 1 case of type 3 failure, 1 case of type 4 failure), 3 cases of revision while keeping the original prosthesis (2 cases of type 1 failure, 1 case of type 5 failure). The overall MSTS score was 24±3. The MSTS scores were 24±3, 25±3, and 23±3 in patients whose reconstruction sites located in proximal femur, distal femur, and proximal tibia, respectively, showing no significant difference ( F=3.014, P=0.052). Conclusion: The short-term follow-up showed a lower incidence of complications and good function for MEGASYSTEM-C non-cement modular prosthesis system in treatment of bone defects after lower limb tumor resection. The main factors affecting the early survival of prosthesis were tumor progression and infection.

14.
Chem Commun (Camb) ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31512684

RESUMO

Correction for 'Synthesis of hydrophobic and hydrophilic TiO2 nanofluids for transformable surface wettability and photoactive coating' by Jie Hu et al., Chem. Commun., 2019, 55, 9275-9278.

15.
J Clin Gastroenterol ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31513027

RESUMO

GOAL: The goal of this study was to compare the efficacy and safety of endoscopic submucosal tunnel dissection (ESTD) with endoscopic submucosal dissection (ESD) for the removal of early-stage esophageal squamous cancer wider than or equal to one half the circumference of the esophagus. BACKGROUND: Although ESD has been successfully applied for resection of early-stage esophageal cancer, there are still technical challenges and postoperative stenosis when it is applied to treat large lesions. PATIENTS AND METHODS: A total of 40 patients with early-stage esophageal cancer wider than or equal to one half its circumference were enrolled in this study and randomly assigned to an ESTD or ESD group for treatment of esophageal superficial squamous cell carcinoma. All of the patients received oral steroids after endoscopic dissection. We then compared the 2 groups in terms of average operating time, dissection speed, en bloc resection rate, R0 resection rate, and complications during a 1-year follow-up period. RESULTS: The dissection speed in the ESTD group was significantly faster than that in the ESD group (P=0.047). There were no significant differences in operating time, en bloc resection rates, or R0 resection rates between the ESTD and ESD group (P=0.319, 1.000, 1.000, respectively). There were also no significant differences in perforation, bleeding, or stenosis rates between the ESTD and ESD group (P=1.000, 0.748, 1.000, respectively). CONCLUSION: Both ESTD and ESD are safe and effective therapies for early-stage esophageal cancer wider than or equal to one half the esophageal circumference. The dissection speed of ESTD is faster than that of ESD.

16.
Spectrochim Acta A Mol Biomol Spectrosc ; 225: 117482, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31472424

RESUMO

Cysteine has a great effect on the physiological and pathological processes, which could bring out various diseases such as skin lesions, edema, hair depigmentation, Alzheimer's, Parkinson's, and liver damage due to the abnormal concentrations of cysteine. Therefore, it is of great impoatance to develop a method for imaging Cys. Herein, a novel fluorescent probe was developed for imaging Cys in vivo specially. This turn-on probe exhibited favorable advantages including large Stokes shift (90 nm), fast response (10 min), good selectivity, low cytotoxicity and so on. Furthermore, the probe could be applied to monitoring cysteine in living HeLa cells, which indicates that this turn-on probe could penetrate viable cell membranes and image Cys over other analystes especially HCy and GSH.

17.
Ecotoxicol Environ Saf ; 184: 109593, 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31479760

RESUMO

Leaf vegetables have strong capabilities to take up cadmium (Cd) compared to other vegetable varieties. Until now, the differences in Cd uptake and accumulation by leaf vegetables from different families and genera and the related health risks were unknown. To remedy this, we studied 71 leaf vegetables (multiple genotypes within 17 categories of vegetables) in soil cultivation experiments (3 Cd treatment levels). Results showed that at 2.12 mg kg-1 Cd treatment, the dry weight of only five genotypic varieties from the families Brassicaceae and Asteraceae significantly decreased compared to the control, suggesting their weak Cd tolerances. Vegetables from the Brassicaceae, Asteraceae, Apiaceae, and Convolvulaceae families had stronger Cd absorption capabilities, whereas those from the Liliaceae and Amaranthaceae families had weaker ones. Cluster analysis found that the 17 vegetable categories could be divided into three groups: vegetables with high Cd accumulation capabilities were Lactuca sativa L.var. ramosa Hort. and Lactuca sativa var. longifoliaf. Lam. Vegetables with moderate Cd accumulation capabilities were bok choy, napa cabbage, choy sum, leaf mustard, Lactuca sativa L., Sonchus oleraceus L., celery, coriander, and water spinach. Vegetables with low Cd accumulation capabilities were cabbage, crown daisy, garlic chive, Allium ascalonicum, Gynura cusimbua, and edible amaranth. Estimated daily intake (EDI) and target hazard quotient (THQ) analysis results showed that 100% genotypes of vegetables from the Apiaceae and Convolvulaceae families had health risks; 100% genotypes of Lactuca sativa L., Sonchus oleraceus L., Lactuca sativa L. var. ramosa Hort., and Lactuca sativa var. longifoliaf. Lam from the Asteraceae family carried high risks. Of vegetables in the Brassicaceae family, 42.9% showed risks. Vegetables from the Amaranthaceae and Liliaceae families, Gynura cusimbua and crown daisy from the Asteraceae family, and cabbage from the Brassicaceae family all displayed relatively low risks (all 100%).

18.
J Eukaryot Microbiol ; 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31419341

RESUMO

We report the morphology and morphogenesis of Urosoma caudata (Ehrenberg, 1833) Berger, 1999 based on in vivo observation and protargol impregnation and provide an improved diagnosis of U. caudata based on previous and current work. Urosoma caudata differs from its congeners mainly by the combination of the following features: tail-like posterior end, colorless cortical granules, and two macronuclear nodules. Urosoma caudata shares most of the ontogenetic features with its congeners: the oral primordium of the opisthe develops apokinetally and the frontal-ventral-transverse cirral anlagen develop in five streaks. However, a unique morphogenetic characteristic is recognizable: the anlagen of three dorsal kineties occur de novo to the left of the parental structures differing from their intrakinetal origin in other Urosoma species. The first record of the 18S rRNA gene sequence for the species is also provided. Phylogenetic analyses based on 18S rRNA gene sequence data suggest that the genus Urosoma is a non-monophyletic group.

19.
JAMA Netw Open ; 2(8): e198243, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31373648

RESUMO

Importance: Ramucirumab, a human IgG 1 antibody against vascular endothelial growth factor receptor 2, has been shown to improve progression-free survival and overall survival in patients with advanced gastric cancer in the second-line setting. Objective: To compare progression-free survival for S-1 and oxaliplatin plus ramucirumab with that for S-1 and oxaliplatin plus placebo in patients with advanced gastric cancer. Design, Setting, and Participants: This phase 2, double-blind randomized clinical trial (RAINSTORM [First-line S-1 Plus Oxaliplatin With or Without Ramucirumab Followed by Paclitaxel Plus Ramucirumab in Patients With Advanced Gastric Cancer]) was conducted from October 12, 2015, to April 11, 2018, at 36 sites in Japan, South Korea, and Taiwan. Participants were chemotherapy-naive patients (n = 189) with metastatic gastric or gastroesophageal adenocarcinoma. Analyses of the full analysis set and safety population were conducted between November 27, 2017, and June 4, 2018. Interventions: Patients randomized to the ramucirumab plus S-1 and oxaliplatin arm received S-1, 80 to 120 mg/d twice daily, on days 1 to 14 and oxaliplatin, 100 mg/m2, on day 1 with ramucirumab, 8 mg/kg, on days 1 and 8 in part A (21-day cycle). Patients randomized to the placebo plus S-1 and oxaliplatin arm received the same S-1 and oxaliplatin dosage as well as placebo on days 1 and 8 in part A. Eligible patients received second-line paclitaxel, 80 mg/m2, on days 1, 8, and 15 and ramucirumab, 8 mg/kg, on days 1 and 15 in part B (28-day cycle). Main Outcomes and Measures: The primary end point was progression-free survival, analyzed using the stratified log-rank test; the hazard ratio (HR) was estimated using the stratified Cox proportional hazards regression model. Secondary end points included overall survival and adverse events. Results: In total, 189 patients were randomized and received treatment: 96 to the ramucirumab plus S-1 and oxaliplatin arm and 93 to the placebo plus S-1 and oxaliplatin arm. Among the 189 patients, 121 (64.0%) were male, and the median (range) age was 62.0 (26-84) years. Median progression-free survival was not prolonged in the ramucirumab plus S-1 and oxaliplatin arm compared with the placebo plus S-1 and oxaliplatin arm (6.34 [80% CI, 5.65-6.93] vs 6.74 [80% CI, 5.75-7.13] months; HR, 1.07; 80% CI, 0.86-1.33; P = .70). Median overall survival was 14.65 (80% CI, 12.39-15.67) months in the ramucirumab plus S-1 and oxaliplatin arm and 14.26 (80% CI, 13.83-17.31) months in the placebo plus S-1 and oxaliplatin arm (HR, 1.11; 80% CI, 0.89-1.40; P = .55). The most commonly reported grade 3 or higher treatment-emergent adverse events in the ramucirumab plus S-1 and oxaliplatin arm in part A were decreased neutrophil count (14 patients [14.6%]), hypertension (10 patients [10.4%]), and anemia (10 patients [10.4%]). Conclusions and Relevance: In this randomized clinical trial, the addition of ramucirumab to first-line S-1 and oxaliplatin treatment did not prolong progression-free survival or overall survival compared with S-1 and oxaliplatin alone among East Asian patients with advanced gastric cancer; no new safety signals for ramucirumab were identified. Trial Registration: ClinicalTrials.gov identifier: NCT02539225.

20.
Medicine (Baltimore) ; 98(35): e17003, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464956

RESUMO

BACKGROUND: The proportion of application of acupuncture for chronic atrophic gastritis (CAG) is increasing over time. We will conduct this study to explore the efficacy and safety of acupuncture as a treatment method for CAG. METHODS: We will go through domestic and foreign databases until July 2019 to identify related randomized controlled trials that explored the effectiveness of acupuncture for CAG. RevMan (V.5.3) and test sequential analysis (V.0.9) will be used for mata-analysis and trial sequential analysis. RESULTS: This study will update previous evidence summaries of acupuncture and determine the efficacy and safety of acupuncture for CAG based on clinical effectiveness rate, clearance of Helicobacter pylori (H pylori) infection, and quality of life and symptom scores. CONCLUSION: This study will determine the evidence for judging whether acupuncture provides benefits in the treatment of CAG, and will support the application of acupuncture in the recovery of patients with CAG. REGISTRATION NUMBER: CRD42019127916.

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