Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 29
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Colloid Interface Sci ; 560: 85-90, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31654898

RESUMO

Mechanical responsive carbon dots (CDs) is a rising star in the family of carbon materials. However, high pressure devices are essential for observing mechanical responses of carbon dots, but they limit the widespread application of mechanical responsive CDs. Two kinds of CDs were prepared using pyrene derivative (pyrene-1-butyric acid, PyBA) as carbon source, and had fluorescence enhancement via simply grinding. An apparent 20-30 times emission enhancement can be observed after the treatment of grinding because of CDs packed structure and morphology changes. To the best of our knownledge, it is the first example of mechanical responsive CDs with fluorescence enhancement without the stimulation of any complex pressure instrumentation. This simple approach could be extended to other carbon sources with π-conjugated structure for building new intelligentized CDs, and provide new routes for the applications of CDs toward mechanical sensing.

2.
Life Sci ; 236: 116918, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610208

RESUMO

Long noncoding RNAs (lncRNAs) are characterized as a group of endogenous RNAs that are more than 200 nucleotides in length and have no protein-encoding function. More and more evidence indicates that lncRNAs play vital roles in various human diseases, especially in tumorigenesis. Focally amplified lncRNA on chromosome 1 (FAL1), a novel lncRNA with enhancer-like activity, has been identified as an oncogene in multiple cancers and high expression level of FAL1 is usually associated with poor prognosis. Dysregulation of FAL1 has been shown to promote the proliferation and metastasis of cancer cells. In the present review, we summarized and illustrated the functions and underlying molecular mechanisms of FAL1 in the occurrence and development of different cancers and other diseases. FAL1 has the potential to appear as a feasible diagnostic and prognostic tool and new therapeutic target for cancer patients though further investigation is needed so as to accelerate clinical application.


Assuntos
Carcinogênese/genética , Carcinogênese/patologia , Regulação Neoplásica da Expressão Gênica , Neoplasias/genética , Neoplasias/patologia , RNA Longo não Codificante/genética , Humanos , Transdução de Sinais
3.
Mol Cell ; 76(4): 646-659.e6, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31543422

RESUMO

Eukaryotic chromosomes contain compartments of various functions, which are marked by and enriched with specific histone modifications. However, the molecular mechanisms by which these histone marks function in chromosome compartmentalization are poorly understood. Constitutive heterochromatin is a largely silent chromosome compartment characterized in part by H3K9me2 and 3. Here, we show that heterochromatin protein 1 (HP1), an H3K9me2 and 3 "reader," interacts with SUV39H1, an H3K9me2 and 3 "writer," and with TRIM28, an abundant HP1 scaffolding protein, to form complexes with increased multivalent engagement of H3K9me2 and 3-modified chromatin. H3K9me2 and 3-marked nucleosomal arrays and associated complexes undergo phase separation to form macromolecule-enriched liquid droplets. The droplets are reminiscent of heterochromatin as they are highly dense chromatin-containing structures that are resistant to DNase and exclude the general transcription factor TFIIB. Our data suggest a general mechanism by which histone marks regulate chromosome compartmentalization by promoting phase separation.

4.
Biomater Sci ; 7(11): 4730-4737, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31497814

RESUMO

Electrical stimulation (ES) exhibits a positive role in promoting the cell activity of osteoblasts. Conducting polymers have the advantages of biocompatibility, good environmental stability and easy synthesis, which have been widely used as charge carriers for electrical stimulation; moreover, considering clinical applications, biomaterial-related infection is an important issue that needs to be solved. Thus, conducting polymers with both antibacterial and osteogenic properties are highly demanded for effect repair. However, it remains a challenge to combine these two characteristics efficiently in a simple way. Herein, an Ag-loaded poly(amide-amine) dendrimer was prepared by a simple chemical reduction procedure, which acted as a dopant for the polymerization of polyaniline (PANI) on biomedical titanium (Ti) sheets. The obtained PANI coating showed outstanding antibacterial properties against Gram-negative (E. coli) and Gram-positive (S. aureus) microbes with a 1000-fold increase when compared with that of pure Ti. In addition, note that the polymer coating together with ES facilitated the proliferation and differentiation of MC3T3. The alkaline phosphatase (ALP) activity and intracellular calcium content of the cells showed a 19.09% and 24.02% increase, respectively, when compared with the case of electrically stimulated Ti after 12 days. Moreover, the existence of PAMAM facilitated mineralization. The strategy developed herein is simple and can be easily manipulated, which shows potential applications in the coating of implants for hard tissue repair.

5.
ACS Appl Mater Interfaces ; 11(38): 34869-34877, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31502819

RESUMO

Tailoring composition and structure are significantly important to improve the utilization and optimize the performance of the precious Pt catalyst toward various reactions, which greatly relies on the feasible synthesis approach. Herein, we demonstrate that Cu-rich Cu5Pt alloys with unique excavated dodecahedral frame-like structure (Cu5Pt nanoframes) can be synthesized via simply adjusting the amounts of salt precursors and surfactants under hydrothermal conditions. It is established that the presence of hexamethylenetetramine and cetyltrimethylammonium bromide, as well as the selection of a proper Pt/Cu ratio are key for the acquisition of the target product. The immediate appeal of this material stems from frame-like architecture and ultralow Pt content involved, which can be used to greatly improve the utilization efficiency of Pt atoms. When benchmarked against commercial catalysts, the developed Cu5Pt nanostructures display superior electrocatalytic performance toward formic acid oxidation, owing to unique electronic effect and ensemble effect. This work elucidates a promising methodology for the synthesis of Pt-based nanostructures while highlights the significance of composition and structure in electrocatalysis.

6.
Polymers (Basel) ; 11(8)2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31394877

RESUMO

Conventional approaches to synthesize thermoplastic polyurethane (TPU) with excellent robustness are limited by a competing relationship between soft and hard segments for tuning mechanical properties in terms of chain flexibility and micro-phase separation. Herein, we present a facile and effective way of simultaneously improving the tensile strength, elongation, and toughness by constructing dynamic cross-linkages from metal-ligand interaction between Zn2+ and pyridine moiety in backbone of poly(urethane urea) (PUU) derived from 2,6-diaminopyridine and poly(propylene glycol). It was found that a Zn2+/pyridine ratio of 1:4 is the most effective for improving robustness. Specifically, tensile strength, elongation, and toughness could be remarkably increased to 16.0 MPa, 1286%, and 89.3 MJ/m3 with 226%, 29%, and 185% increments compared to uncomplexed PUU, respectively. Results from UV-vis, Fourier transform infrared spectroscopy (FTIR), cyclic tensile tests, and stress relaxation reveal that metal-ligand interaction significantly interferes with the hydrogen bonding of urea groups, thus leading to weakening of stiffness. Furthermore, half of vacant ligands enable dynamic complexation during stretching, which consequently ensures constant noncovalent cross-linkages for constraining mutual chain sliding, contributing to simultaneous improvement of tensile strength, elongation, and toughness. This work provides a promising approach for designing TPU with excellent robustness.

7.
J Microbiol Immunol Infect ; 52(5): 685-692, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31255574

RESUMO

BACKGROUND: Development of an efficacious egg-free mock-up H5N1 vaccine is key to our preparedness against pandemic avian flu. METHODS: This is a single-center, randomized, observer-blinded phase I clinical trial evaluating the safety and immunogenicity of an alum-adjuvanted Madin-Darby canine kidney (MDCK)-derived inactivated whole-virion H5N1 influenza vaccine in healthy adults. Hemagglutination inhibition (HAI) and neutralizing antibody titers were measured using horse and turkey red blood cells (RBCs). RESULTS: Thirty-six adult subjects were randomized to receive two doses of 0.5 mL of the MDCK-derived H5N1 alum-adjuvanted vaccine containing 7.5, 15, or 30 µg of hemagglutinin (HA) 21 days apart. The candidate vaccine was well tolerated and safe across the three dosing groups. The most frequent adverse event was injection site pain (46.5%). Both HAI and neutralizing antibody titers increased after each vaccination in all three dosing groups. The best HAI responses, namely a seroconversion rate of 91.7% and a geometric mean ratio of 9.51 were achieved with the HA dose of 30 µg assayed using horse RBCs at day 42. HAI titers against H5N1 avian influenza virus was significantly higher when measured using horse RBCs compared with turkey RBCs. CONCLUSIONS: This Phase I trial showed the MDCK-derived H5N1 candidate vaccine is safe and immunogenic. The source of RBCs has a significant impact on the measurement of HAI titers (ClinicalTrials.gov number: NCT01675284.).


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Imunogenicidade da Vacina , Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/imunologia , Influenza Humana/prevenção & controle , Segurança , Adjuvantes Imunológicos/efeitos adversos , Adulto , Compostos de Alumínio/administração & dosagem , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Aves , Cães , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/fisiopatologia , Feminino , Testes de Inibição da Hemaglutinação , Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia , Cavalos , Humanos , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/efeitos adversos , Influenza Aviária , Injeções Intramusculares , Células Madin Darby de Rim Canino , Masculino , Pessoa de Meia-Idade , Pandemias/prevenção & controle , Soroconversão , Taiwan , Vacinação/efeitos adversos , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/efeitos adversos , Vacinas de Produtos Inativados/imunologia , Adulto Jovem
8.
Environ Sci Technol ; 53(12): 6964-6971, 2019 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-31084027

RESUMO

Intelligent defect engineering to harness surface molecular processes is at the core of selective oxidation catalysis. Here, we demonstrate that the two-electron-trapped oxygen vacancy (VO) of BiOCl, a prototypical F center (VŐ''), is a superb site to confine O2 toward efficient and selective NO oxidation to nitrate. Stimulated by solar light, VŐ'' accomplishes NO oxidation through a two-electron charging (VŐ'' + O2 → VŐ''-O22-) and subsequent one-electron decharging process (VŐ''-O22- + NO → VO-NO3- + e-). The back-donated electron is retrapped by VO to produce a new single-electron-trapped VO (VO'), simultaneously triggering a second round of NO oxidation (VO'-O2 + NO → VO-NO3-). This unprecedented interfacial charging-decharging scheme alters the peroxide-associated NO oxidation selectivity from NO2 to NO3- with a high efficiency and thus hold great promise for the treatment of risky NO x species in indoor air.


Assuntos
Nitratos , Oxigênio , Catálise , Oxirredução , Luz Solar
9.
Brain Res ; 1715: 41-46, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30703371

RESUMO

Arginine-vasopressin (AVP) is believed to be positively correlated with the brain edema formation, but the underlying mechanism is still unclear. In this study, we explored the role of the V2 receptors antagonist tolvaptan on brain edema following intracerebral hemorrhage (ICH) with a rat model. Animals were randomly given tolvaptan or vehicle through oral gavage at 12 h, 36 h, and 60 h after ICH surgery. Brain swelling (%), brain water content(BWC), neurological scores, Evans blue fluorescence and blood-brain barrier (BBB) tight junction proteins were measured to evaluate the effect of tolvaptan in ICH. We found that tolvaptan alleviated the brain swelling (%), decreased the BWC growth, and attenuated the neurological deficits after ICH (p < 0.05, vs vehicle). What's more, tolvaptan increased the expression of ZO-1 and occludin (p < 0.05, vs vehicle), which might be attributed to the down-regulated effects of tolvaptan on MMP-9. These results provided evidence supporting the use of tolvaptan for ICH-induced brain edema. Large animal experiments are required to further explore the efficacy and mechanism of tolvaptan in ICH treatment.

10.
Dalton Trans ; 48(11): 3560-3565, 2019 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-30785438

RESUMO

A hybrid of ZIF-8 with CdS nanorods could increase the transport efficiency of photo-generated charge carriers and the surface area. Notably, through doping Zn ions with a transition metal, in this work, we fabricated a bimetallic ZnM-ZIF (M = Ni, Cu, or Co)-encapsulated CdS nanorod heterostructure for the first time. Compared with ZIF-8, the bimetallic ZIF exhibited a modulated structure, flat band position, and lower overpotential for the hydrogen evolution reaction. ZnM-ZIF not only improved the transfer of water and light, but also boosted the separation of charge carriers. Consequently, the optimized CdS-ZnM-ZIF samples with Cu, Ni, and Co doping showed corresponding photocatalytic hydrogen activities 44, 92, and 59 times larger than that of pristine CdS nanorods. This work provides a new method for better utilization of porous MOF crystals for photocatalysts.

11.
Sci Rep ; 8(1): 14547, 2018 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-30266996

RESUMO

Interfaces remain one of the major issues in limiting the understanding and designing of polymer nanocomposites due to their complexity and pivotal role in determining the ultimate composites properties. In this study, we take multi-walled carbon nanotubes/silicone elastomer nanocomposites as a representative example, and have for the first time studied the correlation between high-frequency dielectric dispersion and static/dynamic interfacial characteristics. We have found that the interface together with other meso-structural parameters (volume fraction, dispersion, agglomeration) play decisive roles in formulating the dielectric patterns. The calculation of the relaxation times affords the relative importance of interfacial polarization to dipolar polarization in resultant dielectric relaxation. Dielectric measurements coupled with cyclic loading further reveals the remarkable capability of dielectric frequency dispersion in capturing the evolution of interfacial properties, such as a particular interface reconstruction process occurred to the surfactant-modified samples. All these results demonstrate that high-frequency dielectric spectroscopy is instrumental to probing both static and dynamic meso-structural characteristics, especially effective for the composites with relative weak interfaces which remains a mission impossible for many other techniques. The insights provided here based on the analyses of dielectric frequency dispersion will pave the way for optimized design and precise engineering of meso-structure in polymer nanocomposites.

12.
J Asian Nat Prod Res ; 20(11): 1075-1080, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28944690

RESUMO

A new diterpenoid, 17-methyl-8, 13-labdadien-15, 16-olid-19-oic acid methyl ester (1), along with two known compounds 2 and 3, were isolated from the leaves of Platycladus orientalis (L.) Franco. The structures were confirmed based on the analysis of HR-MS, 1D-NMR, and 2D-NMR spectra and the configuration of 1 was confirmed by the single-crystal X-ray diffraction.


Assuntos
Cupressaceae/química , Diterpenos/química , Folhas de Planta/química , Modelos Moleculares , Estrutura Molecular
13.
Dalton Trans ; 46(32): 10553-10557, 2017 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-28725895

RESUMO

Nanoporous Co-doped Zn1-xCdxS were facilely fabricated via adopting ZIFs as templates, and Cd(NO3)2 and thiourea as precursors. The highly porous microstructure and uniform Co-doping of the photocatalyst afford a high H2-production rate (45.2 and 422.2 times larger than those of Zn0.5Cd0.5S and CdS), providing an effective way for the development of high performance nanoporous photocatalysts.

14.
Future Oncol ; 13(16): 1385-1393, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28453299

RESUMO

AIM: This is a pooled subgroup analysis of Asian patients enrolled in two Phase III confirmatory studies comparing proposed biosimilar LA-EP2006 with reference pegfilgrastim in women receiving chemotherapy for breast cancer. PATIENTS & METHODS: Women were randomized to LA-EP2006 (n = 90) or reference (n = 84) pegfilgrastim (Neulasta®, Amgen, Inc., CA, USA) for ≤6 cycles of TAC chemotherapy. Primary end point was duration of severe neutropenia during Cycle 1 (number of consecutive days with absolute neutrophil count <0.5 × 109/l) with equivalence confirmed if 95% CIs were within a ±1-day margin. RESULTS: Mean duration of severe neutropenia (days) in Cycle 1 was 1.36 ± 0.98 (LA-EP2006) versus 1.35 ± 1.06 (reference) (difference 0.01 days; 95% CI: -0.30-0.32, indicating equivalence). CONCLUSION: LA-EP2006 showed similar clinical efficacy and safety compared with reference pegfilgrastim.


Assuntos
Medicamentos Biossimilares/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Filgrastim/uso terapêutico , Fator Estimulador de Colônias de Granulócitos/genética , Polietilenoglicóis/uso terapêutico , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Medicamentos Biossimilares/efeitos adversos , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/patologia , Feminino , Filgrastim/efeitos adversos , Humanos , Pessoa de Meia-Idade , Neutropenia/induzido quimicamente , Polietilenoglicóis/efeitos adversos , Resultado do Tratamento
15.
Sci Rep ; 7: 45505, 2017 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-28378782

RESUMO

DENV-2 spread throughout the tropical and subtropical regions globally, which is implicated in deadly outbreaks of DHF and DSS. Since dengue cases have grown dramatically in recent years, about half of the world's population is now at risk. Our timescale analysis indicated that the most recent common ancestor existed about 100 years ago. The rate of nucleotide substitution was estimated to be 8.94 × 10-4 subs/site/year. Selection pressure analysis showed that two sites 160 and 403 were under positive selection, while E gene is mainly shaped by stronger purifying selection. BSP analysis showed that estimating effective population size from samples of sequences has undergone three obvious increases, additionally, Caribbean and Puerto Rico maintained higher levels of genetic diversity relative to other 6 representative geographical populations using GMRF method. The phylogeographic analysis indicated that two major transmission routes are from South America to Caribbean and East&SouthAsia to Puerto Rico. The trunk reconstruction confirmed that the viral evolution spanned 50 years occurred primarily in Southeast Asia and East&South Asia. In addition, phylogeographic association-trait analysis indicated that the viral phenotypes are highly correlated with phylogeny in Nicaragua and Puerto Rico (P < 0.05).


Assuntos
Vírus da Dengue/classificação , Vírus da Dengue/genética , Dengue/virologia , Evolução Molecular , Variação Genética , Genótipo , Dengue/epidemiologia , Vírus da Dengue/isolamento & purificação , Saúde Global , Humanos , Filogeografia
16.
Vet Microbiol ; 193: 83-92, 2016 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-27599934

RESUMO

Previous studies lacked of comprehensive analysis about the evolutionary history and phylogeography of global H7N7 viruses. In this study, it is essential to undertake a genome-scale analysis to investigate the evolutionary processes in a global perspective. There was local phylogenetic divergence among eight trees based on individual segments of 132 strains. We detected four reassortments between four distinct groups of viruses divided by HA gene, suggesting intrasubtype reassortment could accelerate the emergence of highly pathogenic virus. The molecular clock estimated that H7N7 virus evolved at a slower evolutionary rate ranged from 1.03E-03 to 2.81E-03subs/site/year. And we also showed that all gene segments of the virus were under strong purifying selection. A total of 11 positively selected sites were detected by at least two out of three methods. We reconstructed the population dynamics of global H7N7 viruses spanning over a century, revealing that temporal trends of the effective population size were consistent with the major epidemics previously reported. Our study adopt a Bayesian phylogeographic approach to investigate the geographic spread of H7N7 viruses, which combined with temporal and spatial information of all sequences. We have confirmed several migration events between different geographic locations supported by higher values of Bayes factor. The diffusion patterns of H7N7 viruses reveal that the virus is more likely to evolve to expand their host ranges even cross the species.


Assuntos
Evolução Molecular , Genoma Viral/genética , Vírus da Influenza A Subtipo H7N7/genética , Influenza Aviária/virologia , Influenza Humana/virologia , Animais , Aves , Epidemias , Especificidade de Hospedeiro , Humanos , Vírus da Influenza A Subtipo H7N7/isolamento & purificação , Influenza Aviária/epidemiologia , Filogenia , Vírus Reordenados , Recombinação Genética , Seleção Genética , Virulência
17.
Chemistry ; 22(24): 8152-7, 2016 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-27136376

RESUMO

Although graphene oxide (GO) has large interlayer spacing, it is still inappropriate to use it as an anode for sodium-ion batteries (SIBs) because of the existence of H-bonding between the layers and ultralow electrical conductivity which impedes the Na(+) and e(-) transformation. To solve these issues, chemical, thermal, and electrochemical procedures are traditionally employed to reduce GO nanosheets. However, these strategies are still unscalable, consume high amounts of energy, and are expensive for practical application. Here, for the first time, we describe the superior Na storage of unreduced GO by a simple and scalable alkali-metal-ion (Li(+) , Na(+) , K(+) )-functionalized process. The various alkali metals ions, connecting with the oxygen on GO, have played different effects on morphology, porosity, degree of disorder, and electrical conductivity, which are crucial for Na-storage capabilities. Electrochemical tests demonstrated that sodium-ion-functionalized GO (GNa) has shown outstanding Na-storage performance in terms of excellent rate capability and long-term cycle life (110 mAh g(-1) after 600 cycles at 1 A g(-1) ) owing to its high BET area, appropriate mesopore, high degree of disorder, and improved electrical conductivity. Theoretical calculations were performed using the generalized gradient approximation (GGA) to further study the Na-storage capabilities of functionalized GO. These calculations have indicated that the Na-O bond has the lowest binding energy, which is beneficial to insertion/extraction of the sodium ion, hence the GNa has shown the best Na-storage properties among all comparatives functionalized by other alkali metal ions.

18.
Postgrad Med ; 128(4): 364-70, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27116910

RESUMO

OBJECTIVES: Lorcaserin is a selective 5-HT2C (5-hydroxytryptamine 2C) receptor agonist indicated for weight management. Here, we assess the impact of lorcaserin on progression from prediabetes to type 2 diabetes (T2D) and on reversion from prediabetes to euglycemia. METHODS: This is a post hoc analysis of pooled data from two Phase 3 studies, BLOOM and BLOSSOM (N = 6136), evaluating the impact of lorcaserin on weight and glycemic parameters over 52 weeks in the subpopulation of obese/overweight subjects with prediabetes, alternately defined by fasting plasma glucose (FPG) 100-125 mg/dl or glycated hemoglobin (HbA1c) 5.7-6.4% at baseline. RESULTS: At Week 52, in the subpopulation with prediabetes, nearly twice as many lorcaserin-treated subjects achieved ≥5% weight loss versus placebo (HbA1c: 55.6% vs. 27.5%, p < 0.001; FPG: 52.8% vs. 28.8%, p < 0.001), and a significantly lower percentage of lorcaserin-treated subjects progressed to T2D versus placebo based on HbA1c (lorcaserin 3.2%, placebo 5.0%, p = 0.032) but not FPG (lorcaserin 1.6%, placebo 2.6%, p = 0.227). A significantly greater proportion of lorcaserin-treated subjects versus placebo also reverted to euglycemia based on both HbA1c (lorcaserin 40%, placebo 29.5%, p < 0.001) and FPG (lorcaserin 52.4%, placebo 46.5%, p = 0.047). CONCLUSION: In subjects with prediabetes, lorcaserin may contribute to weight loss and improve glycemic parameters, and thus may help with preventing progression to T2D and promoting reversion to euglycemia. CLINICAL TRIAL REGISTRATION: www.clinicaltrials.gov identifiers are NCT00395135 (BLOOM) and NCT00603902 (BLOSSOM).


Assuntos
Benzazepinas/uso terapêutico , Glicemia/metabolismo , Diabetes Mellitus Tipo 2/prevenção & controle , Hemoglobina A Glicada/metabolismo , Estado Pré-Diabético/tratamento farmacológico , Agonistas do Receptor 5-HT2 de Serotonina/uso terapêutico , Adulto , Ensaios Clínicos Fase III como Assunto , Progressão da Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Multicêntricos como Assunto , Estado Pré-Diabético/sangue , Ensaios Clínicos Controlados Aleatórios como Assunto
19.
Molecules ; 21(1): E36, 2015 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-26712733

RESUMO

Quantum dot (QD) sensitization can increase the light absorption and electronic transmission of photocatalysts. However, limited studies have been conducted on the photocatalytic activity of photocatalysts after modification by noble metal QDs. In this study, we developed a simple method for fabricating Pd-QD-modified g-C3N4. Results showed that the modification of Pd-QDs can improve the NO photocatalytic oxidation activity of g-C3N4. Moreover, Pd-QD modification changed the NO oxidation mechanism from the synergistic action of h⁺ and O2(-) to the single action of ·OH. We found that the main reason for the mechanism change was that Pd-QD modification changed the molecular oxygen activation pathway from single-electron reduction to two-electron reduction. This study can not only develop a novel strategy for modifying Pd-QDs on the surface of photocatalysts, but also provides insight into the relationship between Pd-QD modification and the NO photocatalytic oxidation activity of semiconductor photocatalysts.


Assuntos
Nitrilos/química , Paládio/química , Pontos Quânticos/química , Catálise , Oxirredução , Processos Fotoquímicos , Difração de Raios X
20.
J Pharm Anal ; 5(1): 12-17, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29403910

RESUMO

A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was established to determine 2-oxo-clopidogrel, a crucial intermediate metabolite in human plasma. A chromatographic separation was performed on a Sapphire C18 column following a liquid-liquid extraction sample preparation with methyl t-butyl ether. Detection was carried out on a triple quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) with an electrospray ionization (ESI) mode. The method was validated in terms of specificity, accuracy, precision and limit of quantification. The calibration curves ranged from 0.50 to 50.0 ng/mL with good linearity. The stability was fully validated with addition of 1,4-dithio-DL-threitol (DTT) into the plasma sample prior to and in the preparation procedure. The validated method was proved to be suitable for use in pharmacokinetic study after single oral administration of 75 mg clopidogrel tablets in human subjects, which could make contribution to intensive study of the clinical drug-drug interactions of clopidogrel and individual treatment.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA