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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(11): 1103-1110, 2021 Nov 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34753541

RESUMO

OBJECTIVES: To study the effect of probiotics combined with applied behavior analysis (ABA) in the treatment of children with autism spectrum disorder (ASD). METHODS: A total of 41 children with ASD who attended the Affiliated Hospital of Jiangsu University from May 2019 to December 2020 were enrolled and randomly divided into an observation group with 21 children and a control group with 20 children. The children in the observation group were given oral probiotics combined with ABA intervention, while those in the control group were given ABA intervention alone. The treatment outcomes were compared between the two groups. Autism Treatment Evaluation Checklist (ATEC) was used to evaluate the severity of behavioral symptoms in both groups before intervention and at 3 months after intervention. The fecal samples were collected to analyze the difference in intestinal flora between the two groups based on 16s rRNA high-throughput sequencing. RESULTS: Before intervention, there was no significant difference in the ATEC score between the observation and control groups (P>0.05). At 3 months after intervention, both groups had a significant reduction in the ATEC score, and the observation group had a significantly lower ATEC score than the control group (P<0.05). Before intervention, there was no significant difference in the composition of intestinal flora between the observation and control groups. At 3 months after intervention, there was a significant difference in the composition of intestinal flora between the observation and control groups. Compared with the control group, the observation group had significantly higher relative abundances of Bifidobacterium, Lactobacillus, Coprobacillus, Ruminococcus, Prevotella, and Blautia (P<0.05) and significantly lower relative abundances of Shigella and Clostridium (P<0.05). CONCLUSIONS: Probiotics may improve the effect of conventional ABA intervention in children with ASD by regulating intestinal flora.


Assuntos
Análise do Comportamento Aplicada , Transtorno do Espectro Autista , Probióticos , Transtorno do Espectro Autista/terapia , Criança , Humanos , Estudos Prospectivos , RNA Ribossômico 16S
2.
Artigo em Inglês | MEDLINE | ID: mdl-34705652

RESUMO

Although the spatiotemporal complexity and network connectivity are clarified to be disrupted during the general anesthesia (GA) induced unconsciousness, it remains to be difficult to exactly monitor the fluctuation of consciousness clinically. In this study, to track the loss of consciousness (LOC) induced by GA, we first developed the multi-channel cross fuzzy entropy method to construct the time-varying networks, whose temporal fluctuations were then explored and quantitatively evaluated. Thereafter, an algorithm was further proposed to detect the time onset at which patients lost their consciousness. The results clarified during the resting state, relatively stable fuzzy fluctuations in multi-channel network architectures and properties were found; by contrast, during the LOC period, the disrupted frontal-occipital connectivity occurred at the early stage, while at the later stage, the inner-frontal connectivity was identified. When specifically exploring the early LOC stage, the uphill of the clustering coefficients and the downhill of the characteristic path length were found, which might help resolve the propofol-induced consciousness fluctuation in patients. Moreover, the developed detection algorithm was validated to have great capacity in exactly capturing the time point (in seconds) at which patients lost consciousness. The findings demonstrated that the time-varying cross-fuzzy networks help decode the GA and are of great significance for developing anesthesia depth monitoring technology clinically.


Assuntos
Estado de Consciência , Propofol , Anestesia Geral , Encéfalo , Eletroencefalografia , Entropia , Humanos , Inconsciência
3.
Comput Math Methods Med ; 2021: 9974017, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621329

RESUMO

Medical image quality is highly relative to clinical diagnosis and treatment, leading to a popular research topic of medical image denoising. Image denoising based on deep learning methods has attracted considerable attention owing to its excellent ability of automatic feature extraction. Most existing methods for medical image denoising adapted to certain types of noise have difficulties in handling spatially varying noise; meanwhile, image detail losses and structure changes occurred in the denoised image. Considering image context perception and structure preserving, this paper firstly introduces a medical image denoising method based on conditional generative adversarial network (CGAN) for various unknown noises. In the proposed architecture, noise image with the corresponding gradient image is merged as network conditional information, which enhances the contrast between the original signal and noise according to the structural specificity. A novel generator with residual dense blocks makes full use of the relationship among convolutional layers to explore image context. Furthermore, the reconstruction loss and WGAN loss are combined as the objective loss function to ensure the consistency of denoised image and real image. A series of experiments for medical image denoising are conducted with the denoising results of PSNR = 33.2642 and SSIM = 0.9206 on JSRT datasets and PSNR = 35.1086 and SSIM = 0.9328 on LIDC datasets. Compared with the state-of-the-art methods, the superior performance of the proposed method is outstanding.


Assuntos
Algoritmos , Interpretação de Imagem Assistida por Computador/métodos , Redes Neurais de Computação , Biologia Computacional , Simulação por Computador , Bases de Dados Factuais , Aprendizado Profundo , Humanos , Interpretação de Imagem Assistida por Computador/estatística & dados numéricos , Pulmão/diagnóstico por imagem , Razão Sinal-Ruído , Tomografia Computadorizada por Raios X/estatística & dados numéricos
4.
J Neural Eng ; 18(5)2021 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-34534980

RESUMO

Objective.Unconsciousness is a key feature related to general anesthesia (GA) but is difficult to be evaluated accurately by anesthesiologists clinically.Approach.To tracking the loss of consciousness (LOC) and recovery of consciousness (ROC) under GA, in this study, by investigating functional connectivity of the scalp electroencephalogram, we explore any potential difference in brain networks among anesthesia induction, anesthesia recovery, and the resting state.Main results.The results of this study demonstrated significant differences among the three periods, concerning the corresponding brain networks. In detail, the suppressed default mode network, as well as the prolonged characteristic path length and decreased clustering coefficient, during LOC was found in the alpha band, compared to the Resting and the ROC state. When to further identify the Resting and LOC states, the fused network topologies and properties achieved the highest accuracy of 95%, along with a sensitivity of 93.33% and a specificity of 96.67%.Significance.The findings of this study not only deepen our understanding of propofol-induced unconsciousness but also provide quantitative measurements subserving better anesthesia management.


Assuntos
Estado de Consciência , Propofol , Anestesia Geral , Encéfalo , Humanos , Inconsciência/induzido quimicamente
5.
J Clin Nurs ; 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-34396613

RESUMO

AIMS AND OBJECTIVES: To describe the current situation and analyse influencing factors of resource utilisation in maintenance haemodialysis patients. BACKGROUND: The self-management level of patients undergoing maintenance haemodialysis in China is low. The applicability of the Chronic Illness Resources Survey in Chinese maintenance haemodialysis patients has been validated, and this survey can be used to assess the utilisation of self-management support resources among haemodialysis patients. Determining the influencing factors of resource utilisation can help improve the self-management behaviour of haemodialysis patients and control the progression of the disease. DESIGN: A descriptive study design was chosen. METHODS: A convenience sample of 314 patients undergoing haemodialysis was recruited from dialysis centres in three hospitals in western Xinjiang from June 2018-April 2019. SPSS19.0 software was used to describe the current situation of resource utilisation. Moreover, t test, analysis of variance and multivariate linear regression were applied to explore the influencing factors of patients' resource utilisation. Standards for observational studies STROBE Statement checklist-v4 were chosen on reporting the study process. RESULTS: The average score of the total resource utilisation was 2.95 ± 0.51. The average item score of seven dimensions from high to low was 3.46 for medical staff, 3.32 for personal, 3.22 for family and friends, 3.16 for work-related, 3.12 for media policy, 2.55 for neighbourhood/community and 2.23 for organisation. Multiple linear regression showed that there have six main factors, which explained 65.4% of the variance of haemodialysis patients' resource utilisation. CONCLUSIONS: The personal, family and friends, work and media and policy subscales were rated the highest, with support from neighbourhood/community and organisation rated the lowest. In general, participants perceived moderate levels of support. Government and healthcare team should pay more attention on haemodialysis patients who had low level of education, poor economic conditions and poor mental condition. RELEVANCE TO CLINICAL PRACTICE: ESRD has caused a burden on healthcare expenditure and has become a critical public health problem worldwide. Self-management is particularly important for dialysis patients which have attracted extensive interest. In the future research, we can use this questionnaire to evaluate the related resources of disease management of dialysis patients, encourage patients to make full use of the resources around them and focus on those patients with low education and monthly income level and bad mental state.

6.
Int J Nanomedicine ; 16: 5101-5115, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34349510

RESUMO

Introduction: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation. Methods: Based on adjusting the mechanical/interfacial properties of hydrogels, multicellular spheroids (MCSs) of DPSCs generated through self-organization. The spheroids were characterized by immunofluorescent staining and flow cytometry. Quantitative real-time polymerase chain reaction, alkaline phosphatase (ALP) activity assay, ALP staining and Alizarin Red S staining were performed to evaluate the osteogenic/odontogenic differentiation of DPSCs with or without magnetic iron oxide nanoparticles (IONPs) induction. Results: MCSs of DPSCs exhibited a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation, along with a stronger osteogenic/odontogenic differentiation ability. Moreover, DPSCs spheroids showed more substantial osteogenic differentiation tendency than the classical two-dimensional cultured DPSCs under the stimulation of magnetic IONPs. Conclusion: Three-dimensional spheroids culture of DPSCs based on composite viscoelastic materials combined with mechanical/magnetic stimulation may provide a theoretical basis for the subsequent development of dentin or bone regeneration technology.


Assuntos
Diferenciação Celular , Polpa Dentária , Nanopartículas de Magnetita , Osteogênese , Células-Tronco , Proliferação de Células , Células Cultivadas , Humanos , Hidrogéis , Esferoides Celulares
7.
Ital J Pediatr ; 47(1): 162, 2021 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-34315511

RESUMO

BACKGROUND: To explore the roles of Toll-like receptor (TLR)2 in Th2 cytokine production and immunoglobulin (Ig) class switching following ovalbumin (OVA) sensitization. METHODS: TLR2-/- and wild-type C57BL/6 mice were sensitized by intraperitoneal injection with OVA. Lung pathology was assessed by hematoxylin and eosin staining. Abundance of interleukin (IL)4, IL5, IL13, and IL21 transcripts in the lungs was quantified by RT-PCR. OVA-specific IgG1, IgG2a, IgG2b, IgE and IgM were quantified by enzyme-linked immunosorbent assay. Phosphorylated signal transducer and activator of transcription (STAT)3 in lung tissue was detected by immunohistochemistry staining and nuclear factor (NF) κB activation was measured by immunofluorescence staining. STAT3 activation was inhibited using cryptotanshinone (CPT) treatment. Germline transcripts (Iµ-Cµ, Iγ-Cγ, Iα-Cα or Iε-Cε), post-recombination transcripts (Iµ-Cγ, Iµ-Cα or Iµ- Cε) and mature transcripts (VHDJH-Cγ, VHDJH-Cα or VHDJH-Cε) were analyzed from splenic B cells of OVA-sensitized wild-type mice (with or without CPT treatment) and TLR2-/- mice (with or without IL21 treatment). RESULTS: The lungs of TLR2-/- mice showed a lesser degree of inflammation than wild-type mice after OVA sensitization. Following OVA sensitization, levels of IL4, IL13, and IL21, but not IL5, were significantly lower in TLR2-/- compared with wild-type mice. Moreover, OVA-specific IgG1 and IgE titers were markedly lower and higher, respectively, in TLR2-/- mice. TLR2 deficiency inhibited STAT3 activation but not NF-κB p65 activation. CPT treatment reduced IgG1 titers via inhibition of Stat3 phosphorylation. Both TLR2 knockout and CPT treatment reduced the frequencies of Iγ1-Cγ1, Iγ3-Cγ3 and Iα-Cα transcripts, but IL21 treatment compensated for the effects of TLR2 deficiency. CONCLUSION: These results suggest a role of TLR2 in restricting OVA-sensitized lung inflammation via promotion of IgG1 and inhibition of IgE class switching regulated by IL21 and STAT3.

8.
Appl Opt ; 60(17): 5124-5133, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34143079

RESUMO

Aero-engine blades are an integral part of the aero-engine, and the integrity of these blades affects the flight performance and safety performance of an aircraft. The traditional manual detection method is time-consuming, labor-intensive, and inefficient. Hence, it is particularly important to use intelligent detection methods to detect and identify damage. In order to quickly and accurately identify the damage of the aero-engine blades, the present study proposes a network based on the Improved Cascade Mask R-CNN network-to establish the damage related to the aero-engine blades and detection models. The model can identify the damage type and locate and segment the area of damage. Furthermore, the accuracy rate can reach up to 98.81%, the Bbox-mAP is 78.7%, and the Segm-mAP is 77.4%. In comparing the Improved Cascade Mask R-CNN network with the YOLOv4, Cascade R-CNN, Res2Net, and Cascade Mask R-CNN networks, the results revealed that the network used in the present is excellent and effective.

9.
Int J Neural Syst ; 31(7): 2150031, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34167448

RESUMO

Decision response and feedback in gambling are interrelated. Different decisions lead to different ranges of feedback, which in turn influences subsequent decisions. However, the mechanism underlying the continuous decision-feedback process is still left unveiled. To fulfill this gap, we applied the hidden Markov model (HMM) to the gambling electroencephalogram (EEG) data to characterize the dynamics of this process. Furthermore, we explored the differences between distinct decision responses (i.e. choose large or small bets) or distinct feedback (i.e. win or loss outcomes) in corresponding phases. We demonstrated that the processing stages in decision-feedback process including strategy adjustment and visual information processing can be characterized by distinct brain networks. Moreover, time-varying networks showed, after decision response, large bet recruited more resources from right frontal and right center cortices while small bet was more related to the activation of the left frontal lobe. Concerning feedback, networks of win feedback showed a strong right frontal and right center pattern, while an information flow originating from the left frontal lobe to the middle frontal lobe was observed in loss feedback. Taken together, these findings shed light on general principles of natural decision-feedback and may contribute to the design of biologically inspired, participant-independent decision-feedback systems.


Assuntos
Tomada de Decisões , Jogo de Azar , Eletroencefalografia , Retroalimentação , Lobo Frontal , Humanos
10.
Zhongguo Fei Ai Za Zhi ; 24(5): 331-337, 2021 May 20.
Artigo em Chinês | MEDLINE | ID: mdl-34034456

RESUMO

BACKGROUND: Epidermal growth factor receptor (EGFR) is the gene with the highest mutation rate in non-small cell lung cancer (NSCLC) patients, and the accurate evaluation of its mutational status can facilitate patients receiving targeted drug therapy and thereby prolong patients' survival. The gene testing platform has adequacy requirements for the specimen quality in order to obtain accurate examination results. It has been reported that the number and proportion of tumor cells in samples will affect the detection rate of EGFR gene mutation. The present study aims to analyze the relationship between the quality of small biopsy specimens of NSCLC and the mutation rate of EGFR gene with amplification refractory mutation system (ARSM) test. METHODS: After collecting the clinical characteristics of 299 cases small biopsy of lung adenocarcinoma, DNA concentration of the specimens and the mutational status of EGFR gene, the number and proportion of tumor cells in HE stained sections evaluated using light microscopy, the relationship between specimen quality and the mutation rate of EGFR gene were analyzed. RESULTS: The mutation rates of EGFR for the groups with tumor cell number ≤500 and >500 were 40.7% (11/27) and 43.8% (119/272) respectively, without significant difference (P=0.764). The mutation rates for the groups with DNA concentration ≤20.4 ng/µL and >20.4 ng/µL were 42.7% (64/150) and 44.3% (66/149) respectively, without significant difference (P=0.776). The mutation rates for the groups with tumor cells proportion ≤30% and >30% were 29.4% (20/68) and 47.6% (110/231) respectively, demonstrating significant difference (P=0.008). Multivariate Logistic analysis showed that male, thyroid transcription factor-1 (TTF-1) negative, smoking history and tumor cell proportion less than 30% were main factors that contributes to the low detection rate of EGFR gene mutation. CONCLUSIONS: After meeting the minimum requirements for detection, the EGFR mutation rate is affected by the proportion of tumor cells in the sample. Therefore, it is necessary to re-evaluate the tumor cell proportion in the last section after the genetic test section. For samples with lower tumor cell proportion, enriching tumor cells through microdissection and other methods is recommended for a more accurate detection result. For specimens that cannot be enriched with tumor cells, circulating tumor DNA (ctDNA) test can be performed as a supplement. If the result is still negative, another biopsy should be considered to obtain enough tumor specimens for molecular testing.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Neoplasias Pulmonares/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Carcinoma Pulmonar de Células não Pequenas/patologia , Análise Mutacional de DNA , Receptores ErbB/genética , Feminino , Humanos , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Mutação , Taxa de Mutação
11.
Dis Markers ; 2021: 6627620, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34007344

RESUMO

Clinical characteristics of intestinal ulcers complicated with Epstein-Barr virus (EBV) infection remain poorly studied. This study is aimed at providing further insight into clinical features of this patient cohort. The presence of serum EBV DNA was assessed in 399 patients with colonic ulcers, of which 30 cases were positive. In EBV-positive patients, the EBV-encoded RNA (EBER) was detected in intestinal tissues of 13 patients (EBER-positive group). The test was negative in 17 patients (EBER-negative group). Acute EBV infection rate in patients with colonic ulcer was 7.52%. Age and sex differences between two groups were not statistically significant. Fever, abdominal lymph node enlargement, and crater-like gouged ulcer morphology were more common in the EBER-positive group (P < 0.05). The albumin level in the EBER-positive group was significantly lower compared to that in the EBER-negative group (P < 0.05). The copy count of EBV DNA in the blood of patients from the EBER-positive group was higher, and the prognosis was worse (P < 0.05). Clinical manifestations were more severe in the EBER-positive group. Endoscopic, histopathological, and biochemical findings were also more serious in this group of patients. The findings point to the importance of assessing the EBER expression in patients with intestinal ulcers of various etiology. EBER positivity should be viewed as a diagnostic marker of more severe condition requiring more aggressive treatment.


Assuntos
Colite Ulcerativa/patologia , Infecções por Vírus Epstein-Barr/patologia , Adolescente , Adulto , Idoso , Colite Ulcerativa/complicações , Colite Ulcerativa/virologia , Colo/patologia , Colo/virologia , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/epidemiologia , Infecções por Vírus Epstein-Barr/virologia , Feminino , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico
12.
Cell Death Dis ; 12(4): 391, 2021 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-33846303

RESUMO

The biological function of TRIM39, a member of TRIM family, remains largely unexplored in cancer, especially in colorectal cancer (CRC). In this study, we show that TRIM39 is upregulated in tumor tissues compared to adjacent normal tissues and associated with poor prognosis in CRC. Functional studies demonstrate that TRIM39 deficiency restrains CRC progression in vitro and in vivo. Our results further find that TRIM39 is a positive regulator of autophagosome-lysosome fusion. Mechanistically, TRIM39 interacts with Rab7 and promotes its activity via inhibiting its ubiquitination at lysine 191 residue. Depletion of TRIM39 inhibits CRC progression and autophagic flux in a Rab7 activity-dependent manner. Moreover, TRIM39 deficiency suppresses CRC progression through inhibiting autophagic degradation of p53. Thus, our findings uncover the roles as well as the relevant mechanisms of TRIM39 in CRC and establish a functional relationship between autophagy and CRC progression, which may provide promising approaches for the treatment of CRC.


Assuntos
Neoplasias Colorretais/metabolismo , Ubiquitina-Proteína Ligases/deficiência , Proteínas rab de Ligação ao GTP/metabolismo , Idoso , Animais , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Progressão da Doença , Humanos , Masculino , Camundongos , Camundongos Nus , Oncogenes , Transfecção , Ubiquitina-Proteína Ligases/metabolismo , Proteínas rab de Ligação ao GTP/genética
13.
BMC Complement Med Ther ; 21(1): 76, 2021 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-33627124

RESUMO

BACKGROUND: Procyanidin B2 (PB2), a unique component of the grape seed and other medicinal plants. PB2 has shown wide anticancer activity in various human cancer cells. However, it remains unclear about the biological effects and associated mechanisms of PB2 on gastric cancer cells. METHODS: Cell proliferation was measured by CCK8 assay, and cellular lactate dehydrogenase (LDH) release was measured in the culture medium. Cellular apoptosis was observed via TUNEL staining assay and measured by caspase-3 and -9 activities. Autophagy was observed by LC3 staining. Western blot analysis was performed to verify autophagy-associated proteins (Beclin1 and Atg5) and Akt-mTOR pathway. RESULTS: PB2 reduced the viability of BGC-823 and SGC-7901 cells in a concentration-dependent manner. Furthermore, PB2 induced increased apoptosis rate of gastric cancer cells and enhanced caspase-3 and -9 activities. Simultaneously, PB2 triggered autophagy in gastric cancer cells, with enhanced LC3 staining and increased expression of Beclin1 and Atg5, while the inhibition of autophagy by 3-MA reversed the PB2-induced suppression on cell viability. In addition, PB2 significantly decreased p-Akt and p-mTOR protein expression of gastric cancer cells. CONCLUSION: PB2 exerts anti-proliferative and apoptotic effects and induces autophagy by modulating Akt/mTOR signaling pathway. PB2 may be developed as a potential therapeutic drug for gastric cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Autofagia/efeitos dos fármacos , Biflavonoides/farmacologia , Catequina/farmacologia , Proantocianidinas/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Gástricas/fisiopatologia , Serina-Treonina Quinases TOR/metabolismo , Apoptose/efeitos dos fármacos , Caspase 3/genética , Caspase 3/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Proteínas Proto-Oncogênicas c-akt/genética , Transdução de Sinais/efeitos dos fármacos , Neoplasias Gástricas/genética , Neoplasias Gástricas/metabolismo , Serina-Treonina Quinases TOR/genética
14.
Ital J Pediatr ; 47(1): 3, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413556

RESUMO

OBJECTIVES: This study aimed to compare the clinical features and laboratory tests of infectious mononucleosis (IM) and hemophagocytic syndrome (HLH) caused by Epstein-Barr virus (EBV) in 1-3-year-old children and to explore the risk factor of HLH caused by EBV (EBV-HLH). METHODS: The clinical data of 92 children with EBV infection admitted in our hospital from 2011 to 2019 were collected; 61 cases were diagnosed as EBV-IM, and 31 cases were diagnosed as EBV-HLH. The subjects' clinical manifestations and laboratory tests were analyzed retrospectively. RESULTS: Compared with EBV-IM patients, EBV-HLH patients had longer durations of fever, both before hospitalization and overall, and a higher probability of hepatomegaly. The levels of ALT, AST, LDH, TG, SF, D-Dimer and the plasma EBV DNA load of EBV-HLH patients were significantly higher than those of EBV-IM patients. The absolute values of CD3+, CD4+, CD8+, NK, and CD3-CD19+ cells and IgA and IgM levels of EBV-HLH patients were significantly lower than those of EBV-IM patients. The plasma EBV DNA load was positively correlated with the PT, TT, α-HBDH, AST, LDH, CK, Scr, BUN, UA, TG, and CRP levels in EBV-HLH patients, and the plasma EBV DNA load was positively correlated with the D-Dimer level in the EBV-IM patients. Among the 10 different potential markers, at the cut-off point of 1721.500 µg/L, the sensitivity and specificity of D-Dimer was 88.90 and 90.20%, respectively. CONCLUSION: The D-Dimer level may be a good prognostic indicator of EBV-HLH caused by EBV.


Assuntos
Herpesvirus Humano 4 , Mononucleose Infecciosa/diagnóstico , Linfo-Histiocitose Hemofagocítica/diagnóstico , Linfo-Histiocitose Hemofagocítica/virologia , Pré-Escolar , Feminino , Hospitalização , Humanos , Lactente , Mononucleose Infecciosa/complicações , Linfo-Histiocitose Hemofagocítica/complicações , Masculino , Estudos Retrospectivos , Sensibilidade e Especificidade , Avaliação de Sintomas , Carga Viral
15.
Exp Ther Med ; 20(2): 1379-1384, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32742372

RESUMO

Effect of micro ribonucleic acid (miR)-30 on the proliferation of trophoblasts in preeclampsia (PE) rats through the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway was studied. The miR-30 mimic was transfected into the trophoblast HTR8/SVNEO cell lines. The effects of expression level of miR-30 on the proliferation and hypoxia-induced apoptosis of HTR8/SVNEO cells were detected via methyl thiazolyl tetrazolium (MTT) assay and Annexin V/propidium iodide staining, respectively, using the flow cytometer. A total of 30 pregnant Sprague-Dawley rats were randomly divided into control group (CTL group, n=10), PE rat group (PE group, n=10) and PE + miR-30 Mimic group (PE+agomiR-30 group, n=10) using a random number table. The protein expression levels of phosphorylated ERK (p-ERK)1/2, ERK1/2, proliferating cell nuclear antigen (PCNA) and tubulin were determined using western blot analysis, and the mRNA expression level of ERK1/2 was detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression level of PCNA in tissues was detected via immunohistochemistry. The results of MTT assay showed that the proliferation of HTR8/SVNEO cells significantly declined in hypoxic environment, while miR-30 promoted the proliferation of HTR8/SVNEO cells and alleviated the hypoxia-induced inhibition on cell proliferation. It was found that the trophoblast apoptosis rate was increased in hypoxia group compared with that in CTL group, while it was significantly decreased in miR-30 Mimic group compared with that in hypoxia group. PE group had obviously decreased p-ERK and PCNA expression levels as well as p-ERK/ERK ratio in placental tissues compared with CTL group, while PE+agomiR-30 group had an obviously increased expression level of PCNA as well as p-ERK/ERK ratio in placental tissues compared with PE group. MiR-30 activates the MAPK/ERK signaling pathway and increases the expression level of PCNA through raising the p-ERK level and p-ERK/ERK ratio, thereby inhibiting cell apoptosis and promoting cell proliferation.

16.
J Pain Res ; 13: 2003-2012, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32848448

RESUMO

Background: Music has shown positive effects on pain management in previous studies. However, the relationship between musical emotional types and therapeutic effects remains unclear. To investigate this issue, this study tested three typical emotional types of music and discussed their neural mechanisms in relation to pain modulation. Subjects and Methods: In this experiment, 40 participants were exposed to cold pain under four conditions: listening to happy music, listening to neutral music, listening to sad music and no sound. EEG and pain thresholds were recorded. The participants were divided into the remission group and the nonremission group for analysis. Differences among conditions were quantified by the duration of exposure to the pain-inducing stimulus in the remission group. EEG data were obtained using a fast Fourier transform (FFT) and then correlated with the behavioral data. Results: We found that sad music had a significantly better effect on alleviating pain, as a result of brain oscillations in a higher beta band and the gamma band at the O2 and P4 electrodes. The comparison between the remission group and the nonremission group suggested that personality may affect music-induced analgesia, and dominance, liveliness and introvert and extrovert personality traits were associated with pain modulation by sad music. Additionally, in the network analysis, we compared brain networks under the three conditions and discussed the possible mechanisms underlying the better analgesic effect of sad music. Conclusion: Sad music may have a better effect on alleviating pain, and its neural mechanisms are also discussed. This work may help understand the effects of music on pain modulation, which also has potential value for clinical use.

17.
Pathol Res Pract ; 216(9): 153100, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32825965

RESUMO

BACKGROUND: Pulmonary capillary hemangiomatosis (PCH) is a very rare and refractory pulmonary vascular disease that causes pulmonary hypertension. Differentiation of PCH from idiopathic pulmonary arterial hypertension (iPAH) is essential because treatment and prognosis can vary greatly between these two diseases. CASE PRESENTATION: A 20-year-old female and a 33-year-old male both presented with progressive exertional dyspnea and cough. High-resolution computed tomography (HRCT) showed bilateral, diffuse, ill-defined centrilobular nodules of ground-glass opacity, without subpleural thickened septal lines or mediastinal lymphadenopathy. Both cases showed clinical and imaging features characteristic of pulmonary veno-occlusive disease (PVOD) or PCH. The entire EIF2AK4 coding sequence was detected with Sanger sequencing, and no pathogenic EIF2AK4 mutations were identified in either case. Video-assisted thoracoscopic surgery (VATS) was safely performed in both cases, and histopathological examinations of biopsies showed that both patients had PCH. CONCLUSION: Two patients presented with clinical and imaging characteristics suspicious for PVOD/PCH. Despite having no pathogenic EIF2AK4 mutations, both were diagnosed with PCH by VATS lung biopsies. The diagnostic distinction of PCH is important to prompt timely evaluations of patients who may need lung transplantations.


Assuntos
Hemangioma Capilar/genética , Hemangioma Capilar/patologia , Neoplasias Pulmonares/patologia , Proteínas Serina-Treonina Quinases/genética , Grupo com Ancestrais do Continente Asiático , Diagnóstico Diferencial , Feminino , Hemangioma Capilar/diagnóstico , Humanos , Hipertensão Pulmonar/diagnóstico , Hipertensão Pulmonar/genética , Hipertensão Pulmonar/fisiopatologia , Pulmão/patologia , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/genética , Pneumopatia Veno-Oclusiva/diagnóstico , Pneumopatia Veno-Oclusiva/genética , Pneumopatia Veno-Oclusiva/patologia , Adulto Jovem
18.
Neuroreport ; 31(11): 806-813, 2020 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-32576768

RESUMO

Post-stroke cognitive impairment (PSCI) severely affects the quality of a survivor's life, but its neurophysiological basis remains unknown. Neuroinflammation has been considered as an important contributor to PSCI, which could be induced or exacerbated by system inflammation. NACHT-LRR- and pyrin-domain-containing protein 3 (NLRP3) inflammasome is the most widely studied in the initiation of inflammation. Here, using a mouse model of photothrombotic stroke, we demonstrated that NLRP3 activation plays a critical role in PSCI. Intraperitoneal injection of the lipopolysaccharide-activated NLRP3 inflammasome, exacerbated the microglial activation and decreased the number of neurons, impaired the hippocampal neurogenesis, eventually aggravated PSCI. Intraperitoneal injection of MCC950 inhibited the NLRP3 activation, decreased the number of microglia, increased the number of neurons and promoted the hippocampal neurogenesis, eventually improved PSCI. Our results identified NLRP3 inflammasome as an important modifier of neuropathology in PSCI, which could be a could be a potential therapeutic target for PSCI treatment.


Assuntos
Disfunção Cognitiva/imunologia , Inflamassomos/imunologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/imunologia , Neurogênese/fisiologia , Acidente Vascular Cerebral/imunologia , Animais , Disfunção Cognitiva/metabolismo , Modelos Animais de Doenças , Furanos/farmacologia , Indenos/farmacologia , Inflamassomos/efeitos dos fármacos , Inflamassomos/metabolismo , Lipopolissacarídeos/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Neurogênese/efeitos dos fármacos , Acidente Vascular Cerebral/metabolismo , Acidente Vascular Cerebral/patologia , Sulfonamidas/farmacologia
19.
Geriatr Nurs ; 41(5): 641-647, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32387000

RESUMO

Loneliness has been identified as a risk factor for depression, while preparation for future care (PFC) can be a protective factor. Little is known about their complex relationships in older adults in China. This study aimed to explore whether PFC moderated the association between loneliness and depression. A total of 481 older adults aged 60 years and above were recruited in rural Shandong, China. After excluding those whose data missing rates were over 15%, data were analyzed for a total of 436 participants. Loneliness, PFC, and depression were measured. Statistical analyses included descriptive analysis, and moderating effects analyses. Our findings showed that PFC and its related dimensions can moderate the relationship between loneliness and depression. When the level of PFC and its dimensions were higher, the effect of loneliness on depression was weaker. PFC should be taken into consideration when interventions are being developed to reduce depression in older adults.


Assuntos
Planejamento Antecipado de Cuidados , Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Depressão/psicologia , Solidão/psicologia , População Rural , Idoso , Idoso de 80 Anos ou mais , China , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco
20.
Int J Biol Macromol ; 159: 98-107, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32416300

RESUMO

Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and XPS were used to confirm successful preparation of M-LSP. The result is that: M-LSP is apt to adsorb cationic dye. In single system, the super adsorption performance of M-LSP for methylene blue (MB) is depended on the carboxyl content in M-LSP. M-LSP performs its remarkable adsorption performance for MB stably at pH 7.0 ~ 10.0, and the maximum adsorption capacity of M-LSP for MB is up to 613.5 mg/g according to Langmuir isotherm model. The Langmuir isotherm and pseudo-second-order kinetic models are more suitable to descript adsorption process of M-LSP for MB. In binary and ternary system, the M-LSP adsorbs the cationic dyes simultaneously, but selectively adsorbs MB. M-LSP can effectively remove cationic dyes in simulate dyestuff water. Moreover, the removal percentage of regenerated M-LSP decreases only 8.4% after 4 desorption-resorption cycles. The results indicated that M-LSP could be a candidate for remediation of real printing and dyeing or textile wastewater containing cationic dyes.


Assuntos
Química Verde/métodos , Lignina/análogos & derivados , Azul de Metileno/análogos & derivados , Adsorção , Ácidos Carboxílicos/química , Esterificação , Lignina/química , Anidridos Maleicos/química
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