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1.
Anal Chem ; 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34613709

RESUMO

Herein, we designed a dual 3D DNA nanomachine (DDNM)-mediated catalytic hairpin assembly (DDNM-CHA) to construct an electrochemical biosensor for ultrasensitive detection of miRNA, which possesses quite a faster reaction rate and much higher amplification efficiency than those of traditional catalytic hairpin assembly (CHA). Impressively, since the DDNM skillfully increases the local concentration of reactants and decreases the steric hindrance of substrates simultaneously, the DDNM-CHA could be endowed with higher collision efficiency and more effective reaction compared with traditional CHA, resulting in a hyper conversion efficiency up to 2.78 × 107 only in 25 min. This way, the developed DDNM-CHA could easily conquer the main predicaments: long reaction time and low efficiency. As a proof of the concept, we adopt the gold nanoparticles (AuNPs) and the magnetic nanoparticle (Fe3O4) as the kernel of DNM-A and DNM-B, respectively, and harness the magnetic electrode to directly adsorb the products H1-H2/Fe3O4 for constructing an immobilization-free biosensor for high-speed and ultrasensitive detection of miRNA with a detection limit of 0.14 fM. As a result, the DDNM-CHA we developed carves out a new insight to design a functional DNA nanomachine and evolve the analysis method for practical amplification in the sensing area and promotes the deeper exploration of the nucleic acid signal amplification strategy and DNA nanobiotechnology.

2.
Dalton Trans ; 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34505594

RESUMO

The utilization of green and sustainable solar energy via photocatalysis is regarded as a promising strategy to tackle the ever-increasing energy shortage and environmental deterioration. In addition to traditional semiconductor-based photocatalysts, metal-organic frameworks (MOFs), a class of crystalline micro-mesoporous hybrid materials constructed from metal or metal nodes interconnected with multi-dentate organic linkers, are emerging as a new type of photocatalytic material. Post-synthetic modifications (PSM) on MOFs, in which chemical transformations or exchanges are made on pre-synthesized MOF materials, are found to be a powerful strategy for fabricating photoactive MOFs based on already existing MOFs. In this frontier article, different PSM strategies for the development of photoactive MOFs, including coordination on unsaturated metal sites, metalation on open coordinated sites, covalent modifications on ligands, ligand exchange, metal exchange and cavity encapsulation, have been summarized. Our views on the challenges and the direction in developing photocatalytic MOFs by PSM are also addressed. We hope that this frontier article can provide some guidance for rational designing of highly efficient MOF-based photocatalysts via PSM strategies and to stimulate more research interest to be devoted to this promising yet largely unexplored field.

3.
ACS Appl Mater Interfaces ; 13(37): 44086-44095, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34516075

RESUMO

Lipid droplets (LDs) play indispensable roles in numerous physiological processes; hence, the visualization of the dynamic behavior of LDs in living cells is of great importance in physiological and pathological research. In this article, the quantitative structure-activity relationship (QSAR) theory was employed as an effective design strategy for the development of organelle-targeting carbon dots (CDs). The lipid-water partition coefficient (Log P) of the QSAR was adopted as a key parameter to predict the cellular uptake and subcellular localization of CDs in live cells. By carefully adjusting the molecular structure and lipophilicity of the precursors, p-phenylenediamine-derivatized nucleolus-targeting hydrophilic CDs were converted to lipophilic CDs [4-piperidinoaniline (PA) CDs] with inherent LD-targeting performance. The PA CDs were able to indicate the dynamic behavior of LDs and visualize the changes of bisphenol A-induced nonalcoholic fatty liver disease-like changes in a cellular model. The QSAR strategy of CDs demonstrated here is expected to be increasingly exploited as a powerful design tool for developing various organelle-targeting CDs.

4.
Anal Chem ; 93(36): 12434-12440, 2021 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-34473470

RESUMO

The ability to accurately diagnose cancer is the cornerstone of early cancer treatment. The mitochondria in cancer cells maintain a higher pH and lower polarity relative to that in normal cells. A probe that reports signals only when both conditions are met may provide a reliable method for cancer detection with reduced false positives. Here, we construct an AND logic gate fluorescent probe using mitochondrial microenvironments as inputs. Utilizing the hydrolysis of a coumarin scaffold, the probe generates fluorescence signals ("ON") only when high pH (>7.0) and low polarity conditions exist simultaneously. Additionally, the higher mitochondrial membrane potential in cancer cells provides an additional level of selectivity because probe has increased affinity for cancer cell mitochondria. These capabilities endow the probe with a high contrast fluorescence diagnosis ability of cancer at cellular and tissue levels (as high as 51.9 fold), which is far exceeding the clinic threshold of 2.0 fold.


Assuntos
Lógica , Neoplasias , Cumarínicos , Fluorescência , Corantes Fluorescentes , Hidrólise , Neoplasias/diagnóstico
5.
World Neurosurg ; 156: 68-75, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34571241

RESUMO

OBJECTIVE: We sought to provide clinical evidence of the potential influence of ovariectomy (OVX) on intervertebral disk degeneration. METHODS: We retrospectively reviewed patients with a history of OVX who visited our hospital for lower back pain. In addition, 60 age-matched patients without OVX were randomly selected as control subjects. Next, the following demographic data were recorded and compared among groups: age, body mass index, duration of OVX, history of smoking, alcohol use, hypertension, diabetes, cardiocerebrovascular disease, hyperlipemia, osteoporosis, and degenerative spondylolisthesis. Next, the severity of lumbar disk degeneration, evaluated by the modified Pfirrmann grading system, was compared between groups. Data analyses were performed with SPSS 20.0. software. RESULTS: A total of 15 OVX (unilateral, n = 10; bilateral, n = 5) patients were included with a mean age of 62.40 ± 10.64. The average durations of OVX were 21.33 ± 9.24 years. There existed no remarkable intergroup differences in the demographic data (P > 0.05). Overall, the average Pfirrmann grading scores from L1/2 to L5/S1 presented as L1/2 < L2/3 < L3/4 ≤ L5/S1 ≤ L4/5, with no marked differences between groups (P > 0.05). Nevertheless, OVX groups displayed a relatively higher score at each level than non-OVX group. Moreover, the scores from L3/4 to L5/S1 were higher in the bilateral OVX group relative to the unilateral OVX group while they were equal at L1/2 and L2/3. CONCLUSIONS: Our findings demonstrated that OVX contributed to the progression of lumbar disk degeneration to some extent, but it appeared to be a long-term event.

6.
Chemosphere ; 287(Pt 1): 132030, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34461338

RESUMO

A biocomposite (PN-Fe3O4-PEI) was synthesized via the chemical modification of peanut husk (a low-cost adsorbent) with Fe3O4 particles and polyethyleneimine under benign environmental conditions. The modification agents used in this study were observed to overcome the challenges associated with the use pristine peanut husk with a concomitant enhancement in its efficiency as an adsorbent. Results from the characterization studies employed in this study confirmed PN-Fe3O4-PEI to be a crystalline magnetic adsorbent with a mesoporous structure. The adsorption property of the developed material (PN-Fe3O4-PEI) for wastewater treatment was investigated using Chromium (VI), Phosphates (PO43-) and Congo red (CR) as model pollutants. Using the batch method, PN-Fe3O4-PEI exhibited a maximum monolayer adsorption capacity of 58.4, 13.5 and 71.3 mg g-1 for Cr(VI), PO43- (as P g L-1) and CR, respectively and was dependent on temperature and initial adsorbate concentration. Kinetic studies revealed that the Elovich equation, the pseudo-second order kinetic model and double constant equation well described the uptake of Cr(VI), PO43- and CR onto PN-Fe3O4-PEI, respectively. These results may confirm the uptake of these pollutants to be mainly driven by chemical forces. In addition, PN-Fe3O4-PEI was observed to be efficient for the decontamination of the studied pollutants in real water samples as well as exhibit antibacterial properties towards the growth of S. aureus. These properties of PN-Fe3O4-PEI with its other excellent features such as high stability in solution, good regeneration properties and its facile retrieval from the solution using a magnet promote its suitability for practical wastewater treatment.

7.
Anal Chem ; 93(36): 12456-12463, 2021 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-34449199

RESUMO

Molecular probe that enables in vivo imaging is the cornerstone of accurate disease diagnosis, prognostic estimation, and therapies. Although several nucleic acid-based probes have been reported for tumor detection, it is still a challenge to develop programmable methodology for accurately identifying tumors in vivo. Herein, a reconfigurable DNA hybridization-based reaction was constructed to assemble DNAzyme computing that contains an intracellular miRNA-unlocked entropy-driven catalysis module and an endogenous metal ion-responsive DNAzyme module for specific in vivo imaging. By reasonable design, the programmable DNAzyme computing can not only successfully distinguish tumor cells from normal cells but also enable tumor imaging in living mice. Due to its excellent operation with high specificity and sensitivity, this design may be broadly applied in the biological study and personalized medicine.


Assuntos
Técnicas Biossensoriais , DNA Catalítico , MicroRNAs , Animais , DNA Catalítico/metabolismo , Camundongos , Hibridização de Ácido Nucleico
8.
Pain Ther ; 2021 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-34363598

RESUMO

INTRODUCTION: This aim of this study was to delineate current clinical scenarios of painful diabetic peripheral neuropathy (PDN) and associated anxiety and depression among patients in Mainland China, and to report current therapy and clinical practices. METHODS: A total of 1547 participants were enrolled in the study between 14 June 2018 and 11 November 2019. Recruitment was conducted using a multilevel sampling method. Participants' demographics, medical histories, glucose parameters, Douleur Neuropathique 4 Questionnaire (DN4) scores, visual analogue scale (VAS) pain scores, Patient Health Questionnaire 9 (PHQ-9) scores, Generalised Anxiety Disorder 7 (GAD-7) scores and therapies were recorded. RESULTS: The male-to-female ratio was 1.09:1 (807:740), and the mean age at onset was 61.28 ± 11.23 years. The mean DN4 score (± standard deviation) was 4.91 ± 1.88. The frequencies of DN4 sub-item phenotypes were: numbness, 81%; tingling, 68.71%; pins and needles, 62.90%; burning, 53.59%; hypoaesthesia to touch, 50.16%; electronic shocks, 43.31%; hypoaesthesia to pinprick, 37.94%; brushing, 37.82%; painful cold, 29.61%; and itching, 25.86%. Age, diabetic duration, depression history, PHQ-9 score and GAD-7 score were identified as risk factors for VAS pain score. Peripheral artery disease (PAD) was a protective factor for VAS pain score. For all participants currently diagnosed with PDN and for those previously diagnosed PDN, fasting blood glucose (FBG) was a risk factor for VAS; there was no association between FBG and VAS pain score for PDN diagnosed within 3 months prior to recruitment. Utilisation rate of opium therapies among enrolled participants was 0.71% , contradiction of first-line guideline recommendation for pain relief accounted for 9.43% (33/350) and contradiction of second-line guideline recommendation for opium dosage form was 0.57% (2/350). CONCLUSION: Moderate to severe neuropathic pain in PDN was identified in 73.11% of participants. Age, diabetic duration, depression history, PHQ-9 score, GAD-7 score and FBG were risk factors for VAS pain scores. PAD was protective factor. The majority of pain relief therapies prescribed were in accordance with guidelines. TRIAL REGISTRATION: ClinicalTrials.gov identifier, NCT03520608, retrospectively registered, 2018-05-11.

9.
Nat Commun ; 12(1): 4712, 2021 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-34354073

RESUMO

Single-pixel holography (SPH) is capable of generating holographic images with rich spatial information by employing only a single-pixel detector. Thanks to the relatively low dark-noise production, high sensitivity, large bandwidth, and cheap price of single-pixel detectors in comparison to pixel-array detectors, SPH is becoming an attractive imaging modality at wavelengths where pixel-array detectors are not available or prohibitively expensive. In this work, we develop a high-throughput single-pixel compressive holography with a space-bandwidth-time product (SBP-T) of 41,667 pixels/s, realized by enabling phase stepping naturally in time and abandoning the need for phase-encoded illumination. This holographic system is scalable to provide either a large field of view (~83 mm2) or a high resolution (5.80 µm × 4.31 µm). In particular, high-resolution holographic images of biological tissues are presented, exhibiting rich contrast in both amplitude and phase. This work is an important step towards multi-spectrum imaging using a single-pixel detector in biophotonics.


Assuntos
Holografia/métodos , Animais , Encéfalo/anatomia & histologia , Compressão de Dados/métodos , Compressão de Dados/estatística & dados numéricos , Feminino , Holografia/instrumentação , Holografia/estatística & dados numéricos , Processamento de Imagem Assistida por Computador/métodos , Processamento de Imagem Assistida por Computador/estatística & dados numéricos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Nus , Dispositivos Ópticos , Imagem Óptica/instrumentação , Imagem Óptica/métodos , Imagem Óptica/estatística & dados numéricos , Fenômenos Ópticos , Cauda/anatomia & histologia
10.
Life Sci ; 284: 119874, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34390725

RESUMO

AIM: To investigate the effect of 17ß-Estradiol (E2) on intervertebral disc degeneration (IVDD) and the related mechanism. MATERIALS AND METHODS: Immunohistochemistry was used to detect the expression of estrogen receptor ß (ERß) within intervertebral discs of humans and rats. After that, rat IVDD model was established by needle puncture and bilateral ovariectomy. Then, the serum E2 level was detected by enzyme linked immunosorbent assay, and the degree of IVDD was evaluated by X-ray, magnetic resonance imaging, hematoxylin and eosin staining, and Safranin O-Fast Green staining. Finally, we used immunohistochemistry and immunofluorescence staining to determine the effect of E2 on nuclear factor kappa-B (NF-κB) signal pathway both in vivo and in vitro. KEY FINDINGS: We identified that IVDD was associated with lower levels of ERß and ERß levels were inversely correlated with IVDD. The histological staining and radiological results showed that E2 supplement could alleviate IVDD progression. Additionally, immunohistochemistry staining demonstrated that E2 could inhibit nucleus pulposus cell (NPC) apoptosis, matrix metalloproteinases (MMPs) synthesis, and degradation of extracellular matrix (ECM) by inhibiting the activation of NF-κB signal pathway. Furthermore, immunofluorescence staining showed that the above effects of E2 on the NF-κB signal pathway could be blocked by the estrogen receptor antagonist ICI182780 in vitro. Finally, inhibition of NF-κB signal pathway by BAY11-7082 could reduce MMPs synthesis and ECM degradation of NPCs. SIGNIFICANCE: Collectively, these findings indicated that E2 could effectively ameliorate IVDD by inhibiting NPC apoptosis via inhibition of NF-κB signal pathway.


Assuntos
Estradiol/farmacologia , Degeneração do Disco Intervertebral/metabolismo , Degeneração do Disco Intervertebral/patologia , NF-kappa B/metabolismo , Transdução de Sinais , Adolescente , Idoso , Idoso de 80 Anos ou mais , Animais , Apoptose/efeitos dos fármacos , Modelos Animais de Doenças , Receptor beta de Estrogênio/metabolismo , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Feminino , Humanos , Interleucina-1beta/metabolismo , Metaloproteinases da Matriz/metabolismo , Pessoa de Meia-Idade , Modelos Biológicos , Núcleo Pulposo/patologia , Ovariectomia , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Adulto Jovem
11.
Int Immunopharmacol ; 100: 107991, 2021 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-34438336

RESUMO

BACKGROUND: MicroRNAs (miRNAs) are involved in the regulation of osteoclast biology and several pathogenic progression. This study aimed to identify the role of miR-26a in osteoclastogenesis and orthodontically induced inflammatory root resorption(OIIRR). METHODS: Rat orthodontic tooth movement (OTM) model was established by ligating a closed coil spring between maxillary first molar and incisor, and 50 g orthodontic force was applied to move upper first molar to middle for 7 days. Human periodontal ligament (hPDL) cells were isolated from periodontium of healthy donors, and then subjected to compression force (CF) for 24 h to mimic an in vitro OTM model. The levels of associated factors in vivo and in vitro were measured subsequently. RESULT: The distance of tooth movement was increased and root resorption pits were occurred in rat OTM model. The expression of miR-26a was decreased in vivo and vitro experiments. CF treatment enhanced the secretion of inflammatory factors receptor activator of nuclear factor-kappa B ligand (RANKL) and IL-6, osteoclast marker levels, and the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts, while miR-26a overexpression reversed these results. Furthermore, miR-26a overexpression inhibited the osteoclastogenesis and rescued the root resorption in OTM rats through inhibition of Jagged1. Additionally, Runx1 could bind to miR-26a promoter and promote its expression, thereby suppressing the osteoclastogenesis. CONCLUSION: We concluded that Runx1/miR-26a/Jagged1 signaling axis restrained osteoclastogenesis and alleviated OIIRR.

12.
J Am Chem Soc ; 2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34369752

RESUMO

In spite of the excellent electrochemical performance in lithium-ion batteries (LIBs), transition-metal compounds usually show inferior capacity and cyclability in sodium-ion batteries (SIBs), implying different reaction schemes between these two types of systems. Herein, coupling operando magnetometry with electrochemical measurement, we peformed a comprehensive investigation on the intrinsic relationship between the ion-embedding mechanisms and the electrochemical properties of the typical FeS2/Na (Li) cells. Operando magnetometry together with ex-situ transmission electron microscopy (TEM) measurement reveal that only part of FeS2 is involved in the conversion reaction process, while the unreactive parts form "inactive cores" that lead to the low capacity. Through quantification with Langevin fitting, we further show that the size of the iron grains produced by the conversion reaction are much smaller in SIBs than that in LIBs, which may lead to more serious pulverization, thereby resulting in worse cycle performance. The underlying reason for the above two above phenomena in SIBs is the sluggish kinetics caused by the larger Na-ion radius. Our work paves a new way for the investigation of novel SIB materials with high capacity and long durability.

13.
Chem Asian J ; 16(19): 2932-2938, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34296809

RESUMO

Acceptorless photocatalytic dehydrogenation is not only a promising alternative to photocatalytic water splitting for hydrogen generation but also provides a green and sustainable strategy for the synthesis of value-added organic compounds. In this work, Ti3 C2 Tx /CdS nanocomposites were obtained by self-assembly of hexagonal CdS in the presence of preformed Ti3 C2 Tx nanosheets, which serves as a photocatalyst for acceptorless dehydrogenation of biomass-derived furfuryl alcohol (FOL) to furfural (FAL) and furoic acid (FA) in neutral and alkaline medium respectively, with simultaneous generation of stoichiometric hydrogen under visible light. Ti3 C2 Tx MXene acts as an efficient cocatalyst for the photocatalytic dehydrogenation of FOL over CdS, with an optimum performance achieved over 0.50 wt% Ti3 C2 Tx /CdS nanocomposite. This study provides an economic and sustainable strategy for the simultaneous valorization of biomass-derived FOL to produce FAL and FA as well as the production of clean energy hydrogen under mild condition based on noble metal-free semiconductor-based photocatalysts.

14.
Opt Lett ; 46(13): 3095-3098, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34197389

RESUMO

Ultrasound-modulated optical tomography (UOT) images optical contrast deep inside biological tissue. Among existing approaches, camera-based parallel detection is beneficial in modulation depth but is limited to the relatively slow framerate of cameras. This condition prevents such a scheme from achieving maturity to image live animals with sub-millisecond speckle correlation time. In this work, we developed on-axis single-shot UOT by investigating the statistics of speckles, breaking the restriction imposed by the slow camera framerate. As a proof of concept, we experimentally imaged a one-dimensional absorptive object buried inside a moving scattering medium with speckle correlation time down to 0.48 ms. We envision that this single-shot UOT is promising to cope with live animals with fast speckle decorrelation.

15.
Anal Chem ; 93(27): 9568-9574, 2021 07 13.
Artigo em Inglês | MEDLINE | ID: mdl-34210120

RESUMO

In this work, an original rolling-circle strand displacement amplification (RC-SDA) was developed by introducing a circle DNA with two recognition domains as a template instead of the limited liner DNA template in traditional strand displacement amplification (SDA), which displayed much shorter reaction time down to 30 min and quite higher conversion efficiency of more than 1.77 × 108 compared with those of traditional strand displacement amplification (SDA) and could be applied to construct a label-free biosensor for ultrasensitive detection of an HIV DNA fragment. Once the target HIV DNA fragment interacts with the template circle DNA, the RC-SDA could be activated to dramatically output amounts of mimic target DNA with the assistance of the Phi29 DNA polymerase and Nb.BbvCI enzyme. In application, while the output products were captured by the DNA tetrahedral nanoprobe (DTNP) modified electrode, the electrochemical tag silver nanoclusters (AgNCs) on DTNP would be released from the electrode surface, accompanied with an obviously decreased electrochemical signal. This way, the developed signal-off biosensor was successfully applied to realize the rapid and ultrasensitive detection of target HIV DNA fragment with a detection limit down to 0.21 fM, which exploits the new generation of a universal strategy beyond the traditional ones for applications in biosensing assay, clinic diagnosis, and DNA nanobiotechnology.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , DNA/genética , Limite de Detecção , Técnicas de Amplificação de Ácido Nucleico , Prata
16.
Artigo em Inglês | MEDLINE | ID: mdl-34236967

RESUMO

Phase-amplitude coupling (PAC) measures the relationship between the phase of low-frequency oscillations (LFO) and the amplitude of high-frequency oscillations (HFO). It plays an important functional role in neural information processing and cognition. Thus, we propose a novel method based on the Jensen-Shannon (JS) divergence and correlation matrix. The method takes the amplitude distributions of the HFO located in the corresponding phase bins of the LFO as multichannel inputs to construct a correlation matrix, where the elements are calculated based on the JS divergence between pairs of amplitude distributions. Then, the omega complexity extracted from the correlation matrix is used to estimate the PAC strength. The simulation results demonstrate that the proposed method can effectively reflect the PAC strength and slightly vary with the data length. Moreover, it outperforms five frequently used algorithms in the performance against additive white Gaussian noise and spike noise and the ability of detecting PAC in wide frequency ranges. To validate our proposed method with real data, it was applied to analyze the local field potential recorded from the dorsomedial striatum in a male Sprague-Dawley rat. It can replicate previous results obtained with other PAC metrics. In conclusion, these results suggest that our proposed method is a powerful tool for measuring the PAC between neural oscillations.


Assuntos
Encéfalo , Modelos Neurológicos , Algoritmos , Animais , Simulação por Computador , Masculino , Ratos , Ratos Sprague-Dawley
17.
J Biomed Mater Res A ; 109(11): 2369-2380, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34110087

RESUMO

Infection often causes disastrous consequences in all fields of clinical medicine, especially orthopedics. Hence, critical efforts are being made to engineer novel nanomaterials for the treatment of orthopedic infections due to the high biocompatibility and antibacterial properties they possess. The purpose of this study was to investigate the antibacterial effects of magnesium hydroxide (Mg(OH)2 ) nanoparticles (NPs) in vitro and determine their possible mechanisms of action. In this study, Escherichia coli was selected as the pathogenic bacteria and it was found that Mg(OH)2 NPs significantly inhibited the growth of E. coli by promoting nucleic acid leakage, inhibiting protein synthesis, and suppressing the metabolic activity. The minimum inhibitory concentration for these bacteria was determined to be 4.4 µg/ml. In vitro flow cytometry and immunofluorescence tests indicated that Mg(OH)2 NPs induced the macrophages to generate reactive oxygen species to kill the bacteria. To understand the mechanisms involved in this process, western blotting was performed and it was found that Mg(OH)2 NPs activated the phosphatidylinositol-3-kinase/serine-threonine kinase (PI3K/Akt) signaling pathway of macrophages to enhance their phagocytosis with no obvious cytotoxicity. Thus, Mg(OH)2 NPs are a suitable choice to develop promising agents or coating materials for the treatment of clinically widespread infections in view of their safety, biocompatibility, and powerful antibacterial properties.

18.
Artigo em Inglês | MEDLINE | ID: mdl-34110443

RESUMO

The combined pollution of arsenic (As) and cadmium (Cd) are quite common in the polymetallic mining areas located in southern China. In the present study, field experiments were established for the purpose of exploring a simultaneous remediation strategy of rice variety-water management-contaminant immobilization on the As and Cd contamination in paddy soil. The obtained results revealed that the Maba Yinzhan was the best low-accumulation rice variety, with As and Cd concentrations of 0.54 and 0.16 mg kg-1, respectively, in the rice grains. On the basis of water management during heading stage, the treatment using 0.15 wt% lime plus 0.5 wt% ferro-ferric oxide (FO) decreased the As and Cd concentrations by 64.02% and 34.78%, respectively, in the grains. The results of this study potentially provide an effective remediation solution for the As/Cd contaminated soils in polymetallic mining areas.

19.
Hum Vaccin Immunother ; 17(10): 3478-3480, 2021 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-34114926

RESUMO

Vaccines are urgently needed to control the COVID-19 pandemic. To gradually increase the vaccination rate among residents, temporary vaccination clinic for COVID-19 plays an important role. It should be located in an area with convenient transportation and concentrated population. Functional zones including waiting and inquiry, registration and notification, injection, observation and emergency room should be established. All vaccine recipients' information should be uploaded to the national immunization information system. Medical staff at the temporary vaccination clinic should be professionally trained. A cautious disinfection and wiping are essential for the temporary vaccination clinic.


Assuntos
COVID-19 , China/epidemiologia , Humanos , Pandemias , SARS-CoV-2 , Vacinação
20.
Sci Rep ; 11(1): 11366, 2021 05 31.
Artigo em Inglês | MEDLINE | ID: mdl-34059762

RESUMO

The aim of this study is to detect the diagnosis value of neutrophil lymphocyte ratio (NLR) and fibrinogen (FIB) in type B aortic dissection (TBAD) patients. This retrospective observation study consisted patients with TBAD, aortic aneurysm and physical examination between January 1, 2016 and December 31, 2019. Demographic and clinical information after the first admission were collected. Multivariate logistic regression analysis was performed to explore the correlational relationship between NLR, FIB and TBAD. Receiver Operating Characteristic Curve (ROC) was performed to evaluate the diagnostic implication of NLR and FIB in TBAD patients. Six hundred and six patients who were first diagnosed with TBAD were included. Control groups were 202 aortic aneurysm and 140 physical examination subjects. The level of NLR and FIB in aortic dissection patients was significantly higher than aortic aneurysm patients and healthy group (P < 0.001). According to the results of multivariate logistic regression analysis, NLR and FIB were independent risk factors of aortic dissection, and the odds ratio (OR) and 95% confidence interval (CI) value of NLR and FIB were 1.499 (1.126-1.738) and 1.914 (1.475-2.485), respectively. The area under the curve (AUC) was 0.836 of NLR and 0.756 of FIB. NLR and FIB showed high specificity, 89% and 83% respectively. This is the first study provided information on the diagnosis performance of NLR and FIB in TBAD patients. NLR and FIB showed high specificity, which may be a valuable tool for the diagnosis of TBAD.

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