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1.
Steroids ; : 108655, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32439406

RESUMO

The BF3·Et2O-catalysed acetolysis of steroid sapogenins diosgenin, sarsasapogenin and tigogenin in dichloromethane as the solvent instead of acetic anhydride afforded (20S)- and (20R)-22,26-epoxycholestanes (compounds 1 and 2). 22S-23-Acetylsapogenins (compounds 4) were synthesized stereospecifically from 20R-22,26-epoxycholestanes (compounds 2) in good yield. The rearrangement of 22S-23-acetylsapogenins (compounds 4) afforded novel disubstituted dihydropyran furostanol frameworks. Exhaustive NMR characterization of the obtained compounds is provided. Additionally, the structures of the critical compounds (6a and 7a) were unequivocallyconfirmed by single crystal X-ray diffraction studies.

2.
Int J Colorectal Dis ; 31(3): 561-70, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26700099

RESUMO

BACKGROUD: The efficacy and safety of self-expandable metallic stents (SEMSs) as a bridge for patients with acute malignant colorectal obstructions (AMCOs) are still controversial. We conducted this study to evaluate the outcomes of patients with AMCOs treated by different strategies. METHODS: From January 2010 to March 2014, a total of 171 patients with AMCOs from Zhongshan Hospital were retrospectively enrolled in this study. One hundred twenty patients successfully received stent placement followed by one-stage laparoscopic or open resection in the stent group, and 51 patients received emergency operations in the emergency group. RESULTS: The operation duration and postoperative hospital stay were significantly shorter in the stent group (114.51 ± 28.65 vs. 160.39 ± 58.94 min, P < 0.001; 8.00 ± 3.97 vs. 12.59 ± 9.07 days, P = 0.001). The stent group also had significantly reduced intraoperative blood loss and the incidence of postoperative complications compared with the emergency group (61.00 ± 43.70 vs. 121.18 ± 85.90 ml, P < 0.001; 16.7 vs. 37.3%, P = 0.003). Kaplan-Meier survival curves showed that the median survival time in the stent group was significantly longer than that in the emergency group (53 vs. 41 months, P = 0.034). In subgroup analysis of stent group, the stent laparoscopy group had significantly decreased postoperative complications (P = 0.025), and similar long-term survival (P = 0.81). CONCLUSIONS: Stent placement as a bridge to surgery is a safe and feasible procedure and provides significant advantages in terms of short-term outcomes and favorable prognoses for patients with AMCOs. Laparoscopic surgery could be considered as an optimal treatment after stent placement.


Assuntos
Neoplasias Colorretais/complicações , Neoplasias Colorretais/cirurgia , Procedimentos Cirúrgicos Eletivos , Emergências , Obstrução Intestinal/complicações , Obstrução Intestinal/cirurgia , Stents Metálicos Autoexpansíveis , Adulto , Idoso , Procedimentos Cirúrgicos Eletivos/efeitos adversos , Feminino , Humanos , Estimativa de Kaplan-Meier , Laparoscopia/efeitos adversos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Stents Metálicos Autoexpansíveis/efeitos adversos , Resultado do Tratamento , Adulto Jovem
3.
Nat Prod Res ; 28(18): 1446-53, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24749724

RESUMO

Timosaponin B-III (TB-III) is a steroidal saponin isolated from the rhizome of Anemarrhenae asphodeloides (Liliaceae). The biotransformation of TB-III by ß-glucosidase was investigated. Three biotransformation products were isolated and their structures were identified as timosaponin B-III-a (M1), (20R,25S)-5ß-spirostane-3ß-ol-3-O-ß-D-glucopyranosyl-(1 → 2)-ß-D-galacopyanoside (M2) and timosaponin AIII (M3). Then the four compounds were evaluated for their anti-depressive activity in mice by the open field test, tail suspension test and forced swimming test. As a result, TB-III, M1 and M3 exhibited modest anti-depressive activity. Structure-activity relationships were investigated and the preliminary conclusions are summarised as follows: the glycosyl at C-3 and C-26 can increase the activity, the double bond between C-20 and C-22 might be important for the anti-depressive activity, the R-configuration at C-22 and S-configuration at C-20 are necessary for its anti-depressive activity.


Assuntos
Antidepressivos/farmacologia , Liliaceae/química , Saponinas/farmacologia , Esteroides/farmacologia , Animais , Antidepressivos/química , Antidepressivos/isolamento & purificação , Biotransformação , Camundongos , Estrutura Molecular , Rizoma/química , Saponinas/química , Saponinas/isolamento & purificação , Esteroides/química , Esteroides/isolamento & purificação , Relação Estrutura-Atividade
4.
Gene ; 535(1): 17-23, 2014 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-24279998

RESUMO

The cysteine and glycine-rich protein 3 (CSRP3) plays an important role in the myofiber differentiation. Here, we identified five SNVs in all exon and intron regions of the CSRP3 gene using DNA sequencing, PCR-RFLP and forced-PCR-RFLP methods in 554 cattle. Four of the five SNVs were significantly associated with growth performance and carcass traits of the cattle. In addition, we evaluated haplotype frequency and linkage disequilibrium coefficient of five sequence variants. The result of haplotype analysis demonstrated 28 haplotypes present in Qinchuan and two haplotypes in Chinese Holstein. Only haplotypes 1 and 8 were being shared by two populations, haplotype 14 had the highest haplotype frequency in Qinchuan (17.4%) and haplotype 8 had the highest haplotype frequency in Chinese Holstein (94.4%). Statistical analyses of combined genotypes indicated that some combined genotypes were significantly or highly significantly associated with growth and carcass traits in the Qinchuan cattle population. qPCR analyses also showed that bovine CSRP3 gene was exclusively expressed in longissimus dorsi muscle and heart tissues. The data support the high potential of the CSRP3 as a marker gene for the improvement of growth performance and carcass traits in selection programs.


Assuntos
Bovinos/genética , Genótipo , Proteínas com Domínio LIM/genética , Desequilíbrio de Ligação , Proteínas Musculares/genética , Músculos/metabolismo , Polimorfismo de Nucleotídeo Único , Animais , Sequência de Bases , China , Primers do DNA , Éxons , Haplótipos , Íntrons , Reação em Cadeia da Polimerase
5.
J BUON ; 18(3): 689-94, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24065484

RESUMO

PURPOSE: To explore the feasibility and short-term effect of laparoscopy-assisted D2 radical gastrectomy for advanced gastric cancer. METHODS: A total of 239 patients with advanced gastric cancer underwent D2 radical gastrectomy between March 2009 and June 2011, from which 106 patients underwent laparoscopic surgery (laparoscopy group) and 133 patients underwent open surgery (open surgery group). The intraoperative and postoperative condition, number of lymph node removed, complications and mortality rates between the two groups were compared. RESULTS: The operation time (268±51 min) and the number of lymph node removed (29.1+6.1) in the laparoscopy group were comparable with the operation time (268±49 min) and the number of lymph node removed (30.2±7.0) in the open surgery group, while there were significant differences in the intraoperative bleeding (134±66 vs 289±139 ml), intraoperative blood infusion cases (5 vs 19), time to first postoperative flatus (3.4±0.9 vs 5.0±1.4 days), time to first taking liquid food (7.3±1.3 vs 8.1±1.4 days) and postoperative hospital stay (12.8±2.6 vs 14.5±3.1 days) between the two groups (p<0.05). These results favored the laparoscopy group. The incidence of postoperative complications in the laparoscopy and open surgery group were 14.1 and 24.8, respectively (p<0.05). Compared with the open surgery, the laparoscopic surgery significantly reduced the incidence of pulmonary infection (p<0.05). There was no significant difference in the postoperative short-term survival rate between the two groups (p>0.05). CONCLUSION: Laparoscopy-assisted D2 gastrectomy for advanced gastric cancer is advantageous in terms of safety and feasibility, rapid postoperative recovery and few complications. Both groups gave comparable results in terms of lymph node dissection and short-term survival.


Assuntos
Gastrectomia , Laparoscopia , Excisão de Linfonodo , Complicações Pós-Operatórias , Neoplasias Gástricas/cirurgia , Estudos de Viabilidade , Feminino , Seguimentos , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Neoplasias Gástricas/patologia
6.
Gene ; 522(2): 206-13, 2013 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-23537997

RESUMO

The cysteine and glycine-rich protein 1 and 2 genes (CSRP1 and CSRP2) are an effective growth factor in promoting skeletal muscle growth in vitro and vivo. However, in cattle, the information on the CSRP1 and CSRP2 genes is very limited. The aim of this study was to examine the association of the CSRP1 and CSRP2 variants with growth and carcass traits in cattle breeds. Three single nucleotide variants (SNVs) were identified within the bovine CSRP1 gene, whereas CSRP2 gene has not detected any SNVs, using DNA pooled sequencing, PCR-RFLP, and forced PCR-RFLP methods. These SNVs include g. 801T>C (Intron 2), g. 46T>C (Exon 3) and g. 99C>G (Intron 3). Besides, we also investigated haplotype frequencies and linkage disequilibrium (LD) coefficients for three SNVs in all study populations. LD and haplotype structure of CSRP1 were different between breeds. The result of haplotype analysis demonstrated eight haplotype present in QC (Qinchuan) and one haplotype in CH (Chinese Holstein). Only haplotype 1 (TTC), shared by all two populations, comprised 10.74% and 100.00%, of all haplotypes observed in QC and CH, respectively. Haplotype 5 (CTC) had the highest haplotype frequencies in QC (30.98%) and haplotype 1 had the highest haplotype frequencies in CH (100.00%). The statistical analyses indicated that one single SNV and 19 combined haplotypes were significantly or highly significantly associated with growth and carcass traits in the QC cattle population (P<0.05 or P<0.01). Quantitative real-time PCR (qRT-PCR) analyses showed that the bovine CSRP1 and CSRP2 genes were widely expressed in many tissues. The results of this study suggest that the CSRP1 gene possibly is a strong candidate gene that affects growth and carcass traits in the Chinese beef cattle breeding.


Assuntos
Composição Corporal/genética , Bovinos/genética , Proteínas com Domínio LIM/genética , Proteínas Musculares/genética , Proteínas Nucleares/genética , Polimorfismo de Nucleotídeo Único , Criação de Animais Domésticos , Animais , Sequência de Bases , Pesos e Medidas Corporais , Bovinos/crescimento & desenvolvimento , Expressão Gênica , Frequência do Gene , Variação Genética , Genótipo , Haplótipos , Desequilíbrio de Ligação , Fenótipo , Característica Quantitativa Herdável , Análise de Sequência de DNA
7.
Mol Biol Rep ; 40(3): 2335-46, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23196708

RESUMO

The apolipoprotein E (ApoE) gene is an important component of plasma lipoprotein, and Fas apoptosis inhibitory molecule (FAIM) is a novel anti-apoptotic gene. In this study, we researched and discussed seven genes in eight different tissues in Qinchuan cattle by quantitative Real-time PCR. The result of analysis showed that ApoE and FAIM 2 genes had a correlation with muscle and fat. PCR-RFLP was applied to analyze the genetic variations of the ApoE and FAIM 2 genes and verify the effect on growth and carcass traits in a total of 365 Qinchuan cattles. The result of haplotype analysis showed that nine different haplotypes were identified among the four SNPs in ApoE and FAIM 2 genes. The statistical analyses indicated that the four SNPs were significant association with growth and carcass traits (P < 0.05, N = 365); and the four SNPs were significant association between nine combined genotypes of candidate genes and growth and carcass traits. Taken together, our results provide the evidence that polymorphisms in candidate genes are associated with growth and carcass traits in Qinchuan cattle, and may be used as a possible candidate for marker-assisted selection and management in beef cattle breeding program.


Assuntos
Perfilação da Expressão Gênica , Genótipo , Polimorfismo de Nucleotídeo Único , Característica Quantitativa Herdável , Alelos , Animais , Bovinos , Regulação da Expressão Gênica , Frequência do Gene , Haplótipos , Desequilíbrio de Ligação , Especificidade de Órgãos/genética
8.
Anal Chim Acta ; 734: 93-8, 2012 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-22704477

RESUMO

A novel immunoassay for the determination of tumor markers in human serum was established by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation. Based on a sandwich-type immunoassay format, analytes in samples were captured by magnetic beads coated with one monoclonal antibody and "sandwiched" by another monoclonal antibody labeled with europium chelates. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; fluorescence was then measured according to the number of europium ions dissociated. Levels of the model analyte, carcinoembryonic antigen (CEA), were determined in a linear range (1-1000 ng mL(-1)) with a limit of detection of 0.5 ng mL(-1) under optimal conditions. The reproducibility, recovery, and specificity of the immunoassay were demonstrated to be acceptable. To evaluate this novel assay for clinical applications, 239 serum samples were evaluated. Compared with the conventional TRFIA and chemiluminescence immunoassay (CLIA), the correlation coefficients of the developed immunoassay were 0.985 and 0.975, respectively. These results showed good correlation and confirmed that our method is feasible and could be used for the clinical determination of CEA (or other tumor antigens) in human serum.


Assuntos
Biomarcadores Tumorais/sangue , Antígeno Carcinoembrionário/sangue , Fluorimunoensaio/métodos , Separação Imunomagnética , Humanos , Modelos Biológicos , Reprodutibilidade dos Testes , Fatores de Tempo
9.
J Biol Chem ; 287(17): 13899-910, 2012 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-22378788

RESUMO

The establishment of endometrial receptivity is a prerequisite for successful pregnancy, which is controlled by a complex mechanism. MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression. However, the contribution of miRNAs in endometrial receptivity is still unknown. Here we used rhesus monkey as an animal model and compared the endometrial miRNA expression profiles during early-secretory (pre-receptive) phase and mid-secretory (receptive) phase by deep sequencing. A set of differentially expressed miRNAs were identified, 8 of which were selected and validated using quantitative RT-PCR. To facilitate the prediction of their target genes, the 3'-UTRome was also determined using tag sequencing of mRNA 3'-termini. Surprisingly, about 50% of the 10,677 genes expressed in the rhesus monkey endometrium exhibited alternative 3'-UTRs. Of special interest, the progesterone receptor (PGR) gene, which is necessary for endometrial receptivity, processes an ultra long 3'-UTR (~10 kb) along with a short variant (~2.5 kb). Evolutionary analysis showed that the 3'-UTR sequences of PGR are poorly conserved between primates and rodents, suggesting a species-biased miRNA binding pattern. We further demonstrated that PGR is a valid target of miR-96 in rhesus monkey and human but not in rodents, whereas the regulation of PGR by miR-375 is rhesus monkey-specific. Additionally, we found that miR-219-5p regulates PGR expression through a primate-specific long non-coding RNA immediately downstream of the PGR locus. Our study provides new insights into the molecular mechanisms underlying endometrial receptivity and presents intriguing species-specific regulatory roles of miRNAs.


Assuntos
Endométrio/metabolismo , Regulação da Expressão Gênica , Receptores de Progesterona/biossíntese , Regiões 3' não Traduzidas , Animais , Sequência de Bases , Sítios de Ligação , Implantação do Embrião , Feminino , Macaca mulatta , Camundongos , MicroRNAs/metabolismo , Dados de Sequência Molecular , Gravidez , Prenhez , Útero/metabolismo
10.
Planta Med ; 78(1): 18-23, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22002851

RESUMO

In the present study, the anti-diabetic effects of a traditional Chinese medicinal formula extract, TongGuanWan, were investigated in type 2 diabetic animals. It was orally administered to C57BL/KsJ-db/db mice once a day for 4 weeks at the doses of 62, 125, and 250 mg/kg body weight. TongGuanWan significantly lowered the blood glucose and glycosylated haemoglobin levels as well as improved the glucose tolerance in db/db mice. The serum triglyceride levels in the db/db mice were significantly decreased, whereas the high-density lipoprotein cholesterol levels were significantly increased, after treatment with this herbal formula. TongGuanWan also markedly decreased the animals' body weights compared to those of the control db/db group but did not alter food intake. The effects of TongGuanWan were compared to those of the drug rosiglitazone. In addition, five main constituents of TongGuanWan, mangiferin, berberine, cinnamic aldehyde, timosaponin BII, and timosaponin AIII, were quantified using high performance liquid chromatography coupled with a diode array and an evaporative light scattering detector (HPLC-DAD-ELSD). These results suggest that TongGuanWan may be useful for the treatment of type 2 diabetes.


Assuntos
Glicemia/metabolismo , Peso Corporal/efeitos dos fármacos , Diabetes Mellitus Tipo 2/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Hemoglobina A Glicada/metabolismo , Hipoglicemiantes/uso terapêutico , Lipídeos/sangue , Acroleína/análogos & derivados , Acroleína/farmacologia , Acroleína/uso terapêutico , Administração Oral , Animais , Berberina/farmacologia , Berberina/uso terapêutico , HDL-Colesterol/sangue , Cromatografia Líquida de Alta Pressão , Diabetes Mellitus Tipo 2/sangue , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Intolerância à Glucose/sangue , Intolerância à Glucose/tratamento farmacológico , Hipoglicemiantes/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NOD , Camundongos Knockout , Fitoterapia , Rosiglitazona , Saponinas/farmacologia , Saponinas/uso terapêutico , Tiazolidinedionas/farmacologia , Triglicerídeos/sangue , Xantonas/farmacologia , Xantonas/uso terapêutico
11.
Chem Biodivers ; 7(12): 2917-30, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21162005

RESUMO

A rapid, sensitive, and specific method by high-performance liquid chromatography (HPLC) coupled to diode-array detection (DAD) and tandem mass spectrometry (MS) techniques was developed for the identification of absorbed constituents and their metabolites in rats after the oral administration of a Chai-Huang decoction (CHD), which consists of Bupleurum chinense and Scutellaria baicalensis in the proportion 1 : 1 (w/w). By comparing their retention times and MS data with those of authentic compounds and published data, a total of 14 compounds were identified in the CHD samples. In addition, eleven and seven compounds were characterized in the urine and serum samples of the rats, respectively. The results indicated that the main absorbed constituents were chrysin-6-C-arabinosyl-8-C-glucoside, chrysin-6-C-glucosyl-8-C-arabinoside, baicalin, wogonin-5-O-glucoside, oroxylin A-7-O-glucuronide, wogonoside, saikosaponin A, saikosaponin C, saikosaponin D, baicalein, and wogonin. These compounds might be responsible for the curative effects of the CHD. The findings demonstrated that the proposed method could be used to rapidly and simultaneously analyze and screen the multiple absorbed bioactive constituents in a formula of traditional Chinese medicines (TCM). This is very important not only for the pharmaceutical discovery process and the quality control of crude drugs but also to explain the mechanisms of action of TCM.


Assuntos
Bupleurum/química , Medicamentos de Ervas Chinesas/química , Scutellaria baicalensis/química , Administração Oral , Animais , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/administração & dosagem , Flavanonas/urina , Flavonoides/urina , Glucosídeos/urina , Glucuronídeos/urina , Medicina Tradicional Chinesa , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/urina , Ratos , Saponinas/urina , Espectrometria de Massas por Ionização por Electrospray
12.
Biomed Chromatogr ; 24(11): 1147-51, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20120039

RESUMO

To profile the anti-Coxsackie virus B3 constituents of Radix Astragali, an HPLC-DAD-MS(n) analytical method, combined with an in vivo test, has been developed to identify the constituents of the active part, which has been demonstrated to have potency to inhibit the proliferation of virus in cardiac muscle, alleviate infraction in heart and elevate the survival rate of the animal. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 19 compounds, including 11 isoflavonoids and eight saponins, were identified, among which one pterocarpane glucoside was reported for the first time. The present study provides an approach to rapidly screening bioactive constituents in traditional Chinese medicines.


Assuntos
Antivirais/análise , Astrágalo (Planta)/química , Cromatografia Líquida de Alta Pressão/métodos , Extratos Vegetais/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , Antivirais/farmacologia , Infecções por Coxsackievirus/tratamento farmacológico , Infecções por Coxsackievirus/virologia , Enterovirus Humano B/efeitos dos fármacos , Células Hep G2 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Extratos Vegetais/farmacologia
13.
J Pharm Biomed Anal ; 45(5): 793-8, 2007 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-17723287

RESUMO

Four metabolites of mangiferin were firstly isolated and identified from rat urine. The structures of the four metabolites were determined to be 1,3,7-trihydroxyxanthone (M-1), 1,3,6,7-tetrahydroxyxanthone (M-2), 1,3,6-trihydroxy-7-methoxyxanthone (M-3) and 1,7-dihydroxyxanthone (M-4), respectively. A simple and specific analytical method for determination of the four metabolites in rat urine was developed by high performance liquid chromatography (HPLC). Quercetin was employed as an internal standard. The correlation coefficients of the calibration curves were higher than 0.997, both intra- and inter-day precision of four metabolites were determined and their R.S.D. did not exceed 10%. The accuracy and linear range had been investigated in detail. The cumulative urinary excretions of the four metabolites were measured and the possible metabolic pathway of the metabolites was discussed.


Assuntos
Xantonas/isolamento & purificação , Xantonas/urina , Administração Oral , Anemarrhena/anatomia & histologia , Animais , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Estabilidade de Medicamentos , Masculino , Medicina Tradicional Chinesa , Estrutura Molecular , Raízes de Plantas/química , Quercetina/química , Ratos , Ratos Sprague-Dawley , Padrões de Referência , Reprodutibilidade dos Testes , Temperatura , Fatores de Tempo , Xantonas/administração & dosagem , Xantonas/química , Xantonas/metabolismo
14.
Biomed Chromatogr ; 21(6): 655-60, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17370298

RESUMO

Anti-DHBV (duck hepatitis B virus) activity was found in the aqueous extracts of Sophora flavescens Ait. in vivo. Liquid chromatography/electrospray ionization ion trap mass spectrometry was applied to characterize the components in duck serum after oral administration of S. flavescens extract. Oxymatrine (1), sophoranol (2), sophoridine (3) and matrine (4) were identified in the serum. Further research on the four compounds was evaluated for their antiviral activity against HBV (hepatitis B virus) in cell culture. The results suggested that oxymatrine, sophoranol and matrine were the efficacy substances for anti-HBV activity in aqueous extracts of S. flavescens Ait.


Assuntos
Antivirais/administração & dosagem , Cromatografia Líquida de Alta Pressão/métodos , Infecções por Hepadnaviridae/tratamento farmacológico , Vírus da Hepatite B do Pato/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Espectrometria de Massas por Ionização por Electrospray/métodos , Replicação Viral/efeitos dos fármacos , Alcaloides/sangue , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Patos , Flavonoides/sangue , Formazans/química , Formazans/metabolismo , Infecções por Hepadnaviridae/virologia , Vírus da Hepatite B do Pato/isolamento & purificação , Vírus da Hepatite B do Pato/fisiologia , Estrutura Molecular , Quinolizinas/sangue , Sophora/química , Espectrofotometria Ultravioleta/métodos , Sais de Tetrazólio/química , Sais de Tetrazólio/metabolismo
15.
Biomed Chromatogr ; 21(7): 762-7, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17385799

RESUMO

A reversed-phase high-performance liquid chromatographic (HPLC) assay for calycosin-7-O-beta-D-glucopyranoside in rat plasma and urine with solid-phase extraction (SPE) was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile-water (16:84, v/v) at a flow rate of 1.0 mL/min. Detection was set at 280 nm. The limit of quantitation of calycosin-7-O-beta-D-glucopyranoside was 0.2 microg/mL in both plasma and urine. The standard curve was linear from 0.2 to 10.0 microg/mL in plasma, and 0.2 to 5.0 microg/mL in urine. Both intra- and inter-day precision of the calycosin-7-O-beta-d-glucopyranoside were determined and their RSD did not exceed 10%. The method was successfully applied to the analysis of samples obtained from a basic pharmacokinetic study, in which calycosin-7-O-beta-d-glucopyranoside was administered orally to rats.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Glucosídeos/farmacocinética , Isoflavonas/farmacocinética , Animais , Glucosídeos/sangue , Glucosídeos/urina , Isoflavonas/sangue , Isoflavonas/urina , Masculino , Ratos , Ratos Sprague-Dawley , Padrões de Referência , Sensibilidade e Especificidade
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