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1.
Dalton Trans ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34812456

RESUMO

Aqueous zinc-ion batteries (ZIBs) are emerging as promising alternatives among various energy storage devices. However, the lack of research on cathode materials with both high capacity and electrochemical stability restricts widespread applications of ZIBs. Herein, surface chemical reconstruction and partial phosphorization strategies are employed to synthesize MOF-derived hierarchical CoO/Ni2P-Co2P nanosheet arrays on Ni foam substrates as cathodes for ZIBs. The unique hierarchical nanostructure and multiple components with exposed surfaces and rich oxygen vacancies accelerate charge transfer and ion diffusion, expose more active sites, and promote the accessibility between the active materials and electrolyte. The oxide/phosphide composites obtained by novel partial phosphorization achieve a common improvement of performance and stability. As expected, the CoO/Ni2P-Co2P electrode delivers a high specific capacity (370.4 mA h g-1 at 3 A g-1) and excellent rate performance (63.3% retention after a six-fold increase in the current density). Moreover, when employed as the cathode of the CoO/Ni2P-Co2P-30//Zn battery, the assembled battery exhibits a superior specific capacity (322.8 mA h g-1 at 2 A g-1), a long cycle life (104.9% retention after 6000 cycles), a favorable energy density (547.5 W h kg-1) and power density (9.7 kW kg-1). Therefore, this study provides a suitable candidate which meets the requirements of high-performance cathode materials for ZIBs.

2.
Dalton Trans ; 50(45): 16685-16693, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34758054

RESUMO

In this study, we reported the covalent post-synthetic modification (PSM) of a luminescent complex to achieve aggregation-induced emission (AIE), prepared using the Schiff base reaction of TPE-CHO and HLC-NH2, denoted by HLC-NH2-TPE. HLC-NH2 formed a 2D luminescent complex which was constructed using 4,4'-diamino-[1,1'-biphenyl]-2,2'-dicarboxylic acid and zinc ions via a solvothermal reaction. HLC-NH2-TPE inherited the luminescence properties of HLC-NH2 and exhibited noticeable AIE properties in response to environmental viscosities and temperature changes. Interestingly, HLC-NH2-TPE displayed a time-dependent luminescence conversion phenomenon in a mixed solution of DMF/H2O (v : v/1 : 9).

3.
Chem Sci ; 12(35): 11730-11734, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34659708

RESUMO

Molecular face-rotating polyhedra (FRP) exhibit complex stereochemistry, rendering it challenging to manipulate their assembly in a stereoselective manner. In our previous work, stereocontrolled FRP were gained at the cost of losing the confined inner space, which hampers their host-guest interactions and potential applications. Through a rational design approach, herein we demonstrate the successful construction of hollow FRP with high diastereoselectivity. Whereas the [4 + 4] imine condensation of meta-formyl substituted C 3h-symmetric TAT-m and C 3-symmetric Tri-NH2 led to the formation of all feasible FRP-12 diastereoisomers; the para-substituted constitutional isomer, TAT-p, exclusively assembled into a pair of homo-directional enantiomeric FRP-13-CCCC/AAAA with a cavity size larger than 600 Å3. Detailed structural characterizations and theoretical investigations revealed the thermodynamic landscape of FRP assembly can be effectively shaped by modulating the van der Waals repulsive forces among the facial building blocks. Our work provided a novel strategy towards stereospecific assembly of pure organic cages, opening up new opportunities for further applications of these chiral materials.

4.
Medicine (Baltimore) ; 100(40): e27442, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34622861

RESUMO

ABSTRACT: Ankle fractures are the most common intra-articular fractures. Osteoporosis is a common and frequent disease among the elderly with a poor prognosis and high risk of fractured ankles. However, the relationship between vitamin B6 and the incidence of fractured ankles in patients with osteoporosis is unclear.A total of 101 patients with osteoporosis were recruited. Clinical and followed-up information was recorded. And the vitamin B6, albumin, globulin, and hemoglobin in the blood were tested. Pearson's chi-squared and spearman test were performed to analyze the correlation between fractured ankles and relative parameters. Univariate and multivariate logistic regression, receiver operating characteristic curve analysis, univariate and multivariate Cox proportional hazards regression analysis, and Kaplan-Meier method were also performed.There exist strong relation between the expression level of vitamin B6 and fractured ankle (P < .001). The expression of vitamin B6 [Odd ratio (OR) = 12.071, 95% confidence interval (CI): 4.69-31.143, P < .001] has a clear correlation with whether the patients have fractured ankles via the univariate logistic regression analysis. In terms of multivariate logistic regression level, vitamin B6 (OR = 15.384, 95% CI:5.195-45.556, P < .001) was significantly associated with fractured ankle. In addition, expression level of vitamin B6 [hazard ratio (HR) = 11.684, 95% CI: 6.419-21.267, P < .001] was significantly associated with Maintenance time from recovery to recurrence (MRTT) of patients with osteoporosis.Enhanced vitamin B6 is significantly correlated with the poor prognosis of patients with osteoporosis and the increasing incidence of fractured ankles.


Assuntos
Fraturas do Tornozelo/sangue , Osteoporose/sangue , Vitamina B 6/sangue , Adulto , Idoso , Fraturas do Tornozelo/epidemiologia , Biomarcadores/sangue , Causalidade , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Osteoporose/epidemiologia , Estudos Prospectivos , Fatores de Risco
5.
J Am Chem Soc ; 143(39): 16256-16263, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34550674

RESUMO

Ultralong organic phosphorescence (UOP) has aroused enormous interest in recent years. UOP materials are mainly limited to crystals or rigid host-guest systems. Their poor processability and mechanical properties critically hamper practical applications. Here, we reported a series of ultralong phosphorescent foams with high mechanical strength. Phosphorescence lifetime of the foam can reach up to 485.8 ms at room temperature. Impressively, lightweight gelatin foam can bear a compressive pressure of 4.44 MPa. Moreover, phosphorescence emission of polymer foam can be tuned from blue to orange through varying the excitation wavelength. Experimental data and theoretical calculations revealed that ultralong phosphorescence was ascribed to the fixation of multiple hydrogen bonds to the clusters of carbonyl groups. These results will allow for expanding the scope of luminescent foams, providing an ideal platform for developing ultralong phosphorescent materials with high mechanical strength.

6.
Br J Nutr ; : 1-12, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34526168

RESUMO

Chronic inflammation exerts pleiotropic effects in the aetiology and progression of chronic obstructive pulmonary disease (COPD). Glucosamine is widely used in many countries and may have anti-inflammatory properties. We aimed to prospectively evaluate the association of regular glucosamine use with incident COPD risk and explore whether such association could be modified by smoking in the UK Biobank cohort, which recruited more than half a million participants aged 40-69 years from across the UK between 2006 and 2010. Cox proportional hazards models with adjustment for potential confounding factors were used to calculate hazard ratios (HR) as well as 95 % CI for the risk of incident COPD. During a median follow-up of 8·96 years (interquartile range 8·29-9·53 years), 9016 new-onset events of COPD were documented. We found that the regular use of glucosamine was associated with a significantly lower risk of incident COPD with multivariable adjusted HR of 0·80 (95 % CI, 0·75, 0·85; P < 0·001). When subgroup analyses were performed by smoking status, the adjusted HR for the association of regular glucosamine use with incident COPD were 0·84 (0·73, 0·96), 0·84 (0·77, 0·92) and 0·71 (0·62, 0·80) among never smokers, former smokers and current smokers, respectively. No significant interaction was observed between glucosamine use and smoking status (Pfor interaction = 0·078). Incident COPD could be reduced by 14 % to 84 % through a combination of regular glucosamine use and smoking cessation.

7.
Eur J Endocrinol ; 185(5): 765-774, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34524977

RESUMO

Objective: To examine the association of incident type 2 diabetes (T2D) risk with sleep factors, genetic risk, and their combination effects. Design: Large prospective population-based cohort study. Methods: This population-based prospective cohort study included 360 403 (mean (s.d.) age: 56.6 (8.0) years) participants without T2D at baseline from the UK Biobank. Genetic risk was categorised as high (highest quintile), intermediate (quintiles: 2-4), and low (lowest quintile) based on a polygenic risk score for T2D. Sleep scores, including long or short sleep duration, insomnia, snoring, late chronotype, and excessive daytime sleepiness, were categorized as an unfavourable, intermediate, or favourable sleep and circadian pattern. Results: During a median follow-up of 9.0 years, 13 120 incident T2D cases were recorded. Among the participants with an unfavourable sleep and circadian pattern, 6.96% (95% CI: 6.68-7.24%) developed T2D vs 2.37% (95% CI: 2.28-2.46%) of participants with a favourable sleep and circadian pattern (adjusted hazard ratio (HR): 1.53, 95% CI: 1.45-1.62). Of participants with a high genetic risk, 5.53% (95% CI: 5.36-5.69%) developed T2D vs 2.01% (95% CI: 1.91-2.11%) of participants with a low genetic risk (adjusted HR: 2.89, 95% CI: 2.72-3.07). The association with sleep and circadian patterns was independent of genetic risk strata. Participants in the lowest quintile with an unfavourable sleep and circadian pattern were 3.97-fold more likely to develop T2D than those in the lowest quintile with a favourable sleep and circadian pattern. Conclusions: Sleep and circadian patterns and genetic risk were independently associated with incident T2D. These results indicate the benefits of adhering to a healthy sleep and circadian pattern in entire populations, independent of genetic risk.


Assuntos
Ritmo Circadiano/fisiologia , Diabetes Mellitus Tipo 2/epidemiologia , Predisposição Genética para Doença/epidemiologia , Vigilância da População , Transtornos do Sono-Vigília/epidemiologia , Sono/fisiologia , Idoso , Estudos de Coortes , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/fisiopatologia , Feminino , Seguimentos , Predisposição Genética para Doença/genética , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Transtornos do Sono-Vigília/genética , Transtornos do Sono-Vigília/fisiopatologia
8.
Bioresour Technol ; 341: 125747, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34461406

RESUMO

Advanced nitrogen removal of effluent discharged from secondary treatment systems can avoid eutrophication. However, the lack of biodegradable organics limits biodenitrification. Packed bed reactors filled with carriers with different micromagnetic field (MMF) strengths were used to perform tertiary denitrification. The results showed that MMF significantly improved the denitrification performance, especially at low C/N ratios. Total nitrogen (TN) removal was increased by 4.12% with 0.6 mT MMF when C/N = 4 and increased by 7.06% and 8.06% with 0.3 mT and 0.9 mT MMFs when C/N = 3, respectively. Zooglea, Flavobacterium, and Denitratisoma contributed to the advanced denitrification performance under MMF. In addition, 0.6 mT MMF enhanced nitrogen metabolism and ABC transporter protein and two-component system activities of microorganisms under C/N = 4; 0.3 mT and 0.9 mT MMFs increased nitrogen, carbohydrate, and amino acid metabolism and ABC transporter protein activities under C/N = 3. These findings indicate that MMF has great potential for advanced denitrification from secondary effluent.


Assuntos
Carbono , Nitrogênio , Reatores Biológicos , Desnitrificação , Águas Residuárias/análise
9.
Ann Am Thorac Soc ; 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34242153

RESUMO

RATIONALE: Both genetic variants and chronic obstructive pulmonary disease (COPD) contribute to the risk of incident severe coronavirus disease 2019 (COVID-19). Whether genetic risk of incident severe COVID-19 is the same regardless of pre-existing COPD is unknown. OBJECTIVES: In this study, we aimed to investigate the potential interaction between genetic risk and COPD in relation to severe COVID-19. METHODS: We constructed a polygenic risk score (PRS) for severe COVID-19 by using 112 single-nucleotide polymorphisms in 430,582 participants from the UK Biobank study. We examined the associations of genetic risk and COPD with severe COVID-19 by using logistic regression models. RESULTS: Of 430,582 participants, 712 participants developed severe COVID-19 as of February 22, 2021, of whom 19.8% had pre-existing COPD. Compared with participants at low genetic risk, those at intermediate genetic risk (OR, 1.34; 95% CI, 1.09-1.66) and high genetic risk (OR, 1.50; 95% CI, 1.18-1.92) had higher risk of severe COVID-19 (P for trend = 0.001), and the association was independent of COPD (P for interaction = 0.76). COPD was associated with a higher risk of incident severe COVID-19 (OR, 1.37; 95% CI, 1.12-1.67; P = 0.002). Participants at high genetic risk and with COPD had a higher risk of severe COVID-19 (OR, 2.05; 95% CI, 1.35-3.04; P < 0.001) than those at low genetic risk and without COPD. CONCLUSIONS: The PRS which combines multiple risk alleles can be effectively used in screening for high-risk populations of severe COVID-19. High genetic risk correlates with a higher risk of severe COVID-19, regardless of pre-existing COPD.

10.
Front Oncol ; 11: 690275, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34222018

RESUMO

Cervical cancer is the fourth most common cancer worldwide, but its incidence varies greatly in different countries. Regardless of incidence or mortality, the burden of cervical cancer in China accounts for approximately 18% of the global burden. The Chinese Cervical Cancer Clinical Study is a hospital-based multicenter open cohort. The major aims of this study include (i) to explore the associations of therapeutic strategies with complications as well as mid- and long-term clinical outcomes; (ii) to widely assess the factors which may have an influence on the prognosis of cervical cancer and then guide the treatment options, and to estimate prognosis using a prediction model for precise post-treatment care and follow-up; (iii) to develop a knowledge base of cervical clinical auxiliary diagnosis and prognosis prediction using artificial intelligence and machine learning approaches; and (iv) to roughly map the burden of cervical cancer in different districts and monitoring the trend in incidence of cervical cancer to potentially inform prevention and control strategies. Patients eligible for inclusion were those diagnosed with cervical cancer, whether during an outpatient visit or hospital admission, at 47 different types of medical institutions in 19 cities of 11 provinces across mainland China between 2004 and 2018. In a total, 63 926 patients with cervical cancer were enrolled in the cohort. Since the project inception, a large number of standardized variables have been collected, including epidemiological characteristics, cervical cancer-related symptoms, physical examination results, laboratory testing results, imaging reports, tumor biomarkers, tumor staging, tumor characteristics, comorbidities, co-infections, treatment and short-term complications. Follow-up was performed at least once every 6 months within the first 5 years after receiving treatment and then annually thereafter. At present, we are developing a cervical cancer imaging database containing Dicom files with data of computed tomography/magnetic resonance imaging examination. Additionally, we are also collecting original pathological specimens of patients with cervical cancer. Potential collaborators are welcomed to contact the corresponding authors, and anyone can submit at least one specific study proposal describing the background, objectives and methods of the study.

11.
Front Med (Lausanne) ; 8: 684101, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34322501

RESUMO

Some patients retested positive for SARS-CoV-2 following negative testing results and discharge. However, the potential risk factors associated with redetectable positive testing results in a large sample of patients who recovered from COVID-19 have not been well-estimated. A total of 745 discharged patients were enrolled between January 30, 2020, and September 9, 2020, in Guangzhou, China. Data on the clinical characteristics, comorbidities, drug therapy, RT-PCR testing, and contact modes to close contacts were collected. Patients who tested positive for SARS-CoV-2 after discharge were confirmed by guidelines issued by China. The repositive rate in different settings was calculated. Among 745 discharged patients, 157 (21.1%; 95% CI, 18.2-24.0%) tested repositive and the repositive rate was 16.8% (95% CI, 14.1-24.0%) for nasopharyngeal swabs and 9.7% (95% CI, 7.0-12.5%) for anal swabs. Among them, 55 (35.0%) were asymptomatic, 15 (9.6%) had mild symptoms, 83 (52.9%) had moderate symptoms, and 4 (2.6%) had severe symptoms at the first admission. The days from discharge to repositivity was 8.0 (IQR, 8.0-14.0). Most repositive patients were without clinical symptoms, and lymphocyte cell counts were higher than before being discharged. The likelihood of repositive testing for SARS-CoV-2 RNA was significantly higher among patients who were of younger age (OR, 3.88; 95% CI, 1.74-8.66, 0-17 years old), had asymptomatic severity (OR, 4.36; 95% CI, 1.47-12.95), and did not have clinical symptoms (OR, 1.89; 95% CI, 1.32-2.70, without fever). No other positive patients emerged within the families or close contacts of repositive patients. Our findings support prolonged but intermittent viral shedding as the probable cause for this phenomenon; we need to familiarize with the possibility that the virus will remain endemic.

12.
Zhen Ci Yan Jiu ; 46(5): 375-9, 2021 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-34085459

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Ciliao" (BL32) and "Huiyang" (BL35) on the expression of extracellular signal-regulated kinase 1/2 (p-ERK1/2) and cellular oncogene fos (c-fos) phosphorylated of spinal dorsal horn in rats with interstitial cystitis (IC). METHODS: Eighteen female Wistar rats were randomly divided into control, model and EA groups, with 6 rats in each group. The IC model was established by intraperitoneal injection of cyclophosphamide (150 mg/kg). EA (30 Hz, 1 mA) was applied to bilateral BL32 and BL35 for 20 min, once daily for 3 consecutive days. The bladder pain was measured by using a Von Frey at 48 h after modeling and 24 h after EA. The expression levels of p-ERK1/2 and c-fos protein in L6-S1 segment of spinal cord were detected by Western blot, and the expression of p-ERK1/2 and c-fos in the right spinal dorsal horn were displayed by immunofluorescence staining. RESULTS: After modeling, the bladder mechanical pain threshold (PT) was significantly decreased (P<0.05), the protein expression of p-ERK1/2 and c-fos in the spinal cord was increased (P<0.05) and the immunofluorescence surface density of p-ERK1/2 and c-fos in the right dorsal horn of spinal cord was increased (P<0.05) in the model group relevant to the control group. After EA intervention, IC-induced reduction of PT, and increases of the expression of p-ERK1/2 and c-fos as well as immunofluorescence surface density of p-ERK1/2 and c-fos were reversed in the EA group relevant to the model group (P<0.05). CONCLUSION: EA at BL 32 and BL 35 has an analgesic effect in IC rats, which may be related to its effect in down-regulating the expression of p-ERK1/2 and c-fos in spinal dorsal horn.


Assuntos
Cistite Intersticial , Eletroacupuntura , Animais , Feminino , Sistema de Sinalização das MAP Quinases , Proteína Quinase 3 Ativada por Mitógeno/genética , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/metabolismo , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Medula Espinal/metabolismo , Corno Dorsal da Medula Espinal/metabolismo
13.
Eur Respir J ; 2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-34172472

RESUMO

BACKGROUND: Genetic and smoking contribute to chronic obstructive pulmonary disease (COPD), but whether a combined polygenic risk score (PRS) is associated with incident COPD and whether it has a synergistic effect on the smoking remains unclear. We aimed to investigate the association of PRS with COPD and explore whether smoking behaviors could modify such association. METHODS: Multivariable Cox proportional models were used to estimate hazard ratios (HRs) and 95% confidence intervals (95% CIs) for the association of the PRS and smoking with COPD. RESULTS: The study included 439 255 participants (mean age 56.5; 53.9% female), with a median follow-up of 9.0 years. The PRSlasso containing 2.5 million variants showed better discrimination and a stronger association for incident COPD than the PRS279 containing 279 genome-wide significance variants. Compared with the low genetic risk, the HRs of the medium and high genetic risk were 1.39 (95% CI, 1.31-1.48) and 2.40 (95% CI, 2.24-2.56), respectively. The HR of high genetic risk and current smoking was 11.62 (95% CI, 10.31-13.10) times of low genetic risk and never smoking. There were significant interactions between the PRSlasso and smoking status for incident COPD (p for interaction<0.001). From low genetic risk to high genetic risk, the HRs of current smoking increased from 4.32 (95% CI, 3.69-5.06) to 6.89 (95% CI, 6.21-7.64), and the population-attributable risks of smoking increased from 42.7% to 61.1%. CONCLUSION: PRS constructed from millions of variants below genome-wide significance showed significant associations with incident COPD. Participants with a high genetic risk may be more susceptible to developing COPD when exposed to smoking.

14.
Int J Occup Saf Ergon ; : 1-7, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34187328

RESUMO

Boredom is a common workplace problem. Previous research has suggested that repetitive and monotonous work tasks may lead to boredom. However, these tasks have been reduced due to increased workplace automation. Thus, the current cause of boredom may be due to low mental workload. This research developed a general boredom model and compared the effects of feedback type (performance and ranking) and intervention method (game and quiz) on boredom and task performance. Results revealed that the secondary task interventions can reduce boredom and feedback could shorten the response time. Gender also had a significant influence on response time. Notably, results revealed a 4% probability of task failure during the experiment, indicating other interventions are also required. This research indicates that interventions to reduce boredom caused by low mental workload should be designed differently from tasks that have been designed to combat the boredom caused by repetitive and monotonous work.

15.
Neuroimage ; 238: 118268, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34139359

RESUMO

Deception emerges in early childhood and prevails in adults. Activation patterns in previous adults' task-state functional magnetic resonance imaging (fMRI), though sensitive to state honesty on a specific decision, are less reliable reflecting trait honesty. Besides of state honesty, most previous neuroimaging studies about dishonesty suffer the generalization problem due to the major focus on adults with children unexplored. To investigate honesty associated functional brain networks variations, 98 healthy adults (Age: 18-28 y.o.; 49 males and 49 females) were invited to participate in a resting-state functional magnetic resonance imaging (rfMRI) study (Study 1). We investigated how functional connections between the caudate and the medial prefrontal cortex (mPFC) change among adults who differ in self-reported trait honesty. Results showed that adults with higher trait honesty have increased functional connectivity from the caudate to the mPFC, which is identified as an honesty-related hub region in global brain connectivity analysis and connects more tightly to a wide range of brain regions including the amygdala. Study 2 compared functional connectivity between children with high vs. low lying frequencies (Age: 6-16 y.o.; 61 males and 39 females) based on a publicly accessible database of rfMRI. Consistent with findings in adults, increased functional connectivity from the caudate to the mPFC was found in less frequently lying children. Despite different honesty indicators of self-reported honesty trait in adults and parent-reported lying patterns in children, consistent findings have been noted in the two samples with regards to functional connectivity variations between reward-related and self-related brain regions. These findings suggest functional connectivity alterations between the caudate and the mPFC contribute to honesty variations in both adults and children.


Assuntos
Núcleo Caudado/diagnóstico por imagem , Emoções/fisiologia , Córtex Pré-Frontal/diagnóstico por imagem , Adolescente , Adulto , Núcleo Caudado/fisiologia , Criança , Decepção , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Imageamento por Ressonância Magnética , Masculino , Vias Neurais/diagnóstico por imagem , Vias Neurais/fisiologia , Córtex Pré-Frontal/fisiologia , Adulto Jovem
16.
Food Chem ; 363: 130288, 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34120043

RESUMO

Table grape is a popular fruit worldwide. The quality of the appearance of table grapes directly affects their commercial value. Table grape bunches are usually carefully managed during production. At different developmental stages, a large number of grape berries are pruned as waste for commercial appearance, which leads to wasted resources. Ultraviolet-C (UV-C) can regulate the accumulation of secondary metabolites in fruits. In this study, metabolomic profile was combined with transcriptomic analysis technology to explore the value of UV-C in improving the utilization of waste grapes. The berries of the 'Jumeigui' grape were subjected to UV-C radiation treatment in the green-berry stage, veraison stage, and maturation stage. The results showed that UV-C could brown grape berries and decrease their sugar content at different developmental stages. Compared with other samples, those treated with UV-C in the veraison stage had the most upregulated metabolites, while samples in the green-berry stage had the most down-regulated metabolites. UV-C promoted the accumulation of stilbenes and some flavonoids in the berries at each developmental stage (especially at the green-berry and veraison stages). Compared with other stages, UV-C treatment during the veraison stage led to the highest number of upregulated genes related to transcription factors, protein modification, indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellin acid (GA), receptor kinases, and Ascorbic acid/Glutathione (Ascorb/Gluath). UV-C might promote the accumulation of phenolic components by upregulating the expression of their biosynthesis related genes. UV-C may be an effective in vitro approach for improving the application value of waste grape berries by enhancing the accumulation of the nutritious phenolic components.


Assuntos
Vitis , Ácido Abscísico , Frutas/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Transcriptoma , Vitis/genética
17.
Prep Biochem Biotechnol ; : 1-8, 2021 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-34010101

RESUMO

Microbial biomass and waste materials conversion for biochemicals production has been an alternative for energy conservation and emission reduction. While toxic substances in biomass materials and high osmotic pressure formed in fermentation-based systems block the bioconversion processes of microorganisms. In the present study, strain T4 that isolated from tobacco waste could resist toxic inhibitors such as nicotine and was suitable for generation of 2, 3-butanediol (2, 3-BD) with a high concentration of glucose (up to 20%). 30.06 and 1.54 g/L of 2, 3-BD was generated respectively from 50 g/L of tobacco waste with and without 200 g/L glucose after fermentation for 48 h. Besides, the results of biochemical tests showed that it was gram-positive and able to liquefy gelatin, hydrolyze starch and produce catalases. It could utilize glucose but not lactose as carbohydrates during fermentation. The 16S rRNA sequence and systematic analysis revealed that T4 was identified to be a Bacillus amyloliquefaciens (B. amyloliquefaciens). This work presents a promising model microorganism chassis to use the biomass waste for high value-added biochemicals production.

18.
Clin Nutr ; 40(5): 2620-2629, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33933728

RESUMO

BACKGROUND & AIMS: The association between dietary diversity (DD) changes and mortality remains unclear. We aimed to investigate the association between DD changes and all-cause mortality among older people. METHODS: A total of 17,959 participants with a mean age of 84.8 years old were enrolled at baseline. Food groups were collected at baseline and follow-up using simplified food frequency questionnaire (FFQ), and then overall, plant-based and animal-based dietary diversity score (DDS) were calculated. DDS changes were calculated using DDS at baseline and the first follow-up. The association between three DDS changes (overall, plant-based and animal-based DDS) and subsequent all-cause mortality were evaluated. Nonparametrically restricted cubic splines and a multivariable-adjusted Cox proportional hazards model were used to estimate HRs and 95% CIs. RESULTS: We documented 12,974 deaths over a 129,590 person-years of follow up. Compared with high-to-high DDS pattern, participants with lower overall DDS patterns had increased mortality risk with HRs (95%CI) of 1.39 (1.29-1.49), 1.53 (1.37-1.70), 1.38 (1.18-1.60) and 1.55 (1.31-1.83) for medium-to-medium, low-to-low, low-to-high and high-to-low patterns, respectively. And compared with high-to-high DDS pattern, the estimates were 1.34 (1.23-1.46), 1.49 (1.35-1.65), 1.43 (1.23-1.67) and 1.62 (1.40-1.88) for plant-based DDS, and 1.23 (1.15-1.31), 1.29 (1.20-1.40), 1.24 (1.12-1.37) and 1.28 (1.15-1.44) for animal-based DDS for medium-to-medium, low-to-low, low-to-high and high-to-low patterns, respectively. There was a U-shaped association between DDS change scores and mortality, and compared with participants with whose DDS remained stable, those with extreme declines and extreme improvements had higher risks of mortality with HRs (95% CI) of 1.15 (1.09-1.22) and 1.11 (1.04-1.17). CONCLUSIONS: Maintaining a lower DDS, extreme declines and extreme improvements in DDS were all associated with an increased risk of all-cause mortality.


Assuntos
Dieta/classificação , Idoso , Idoso de 80 Anos ou mais , Causas de Morte , Estudos de Coortes , Inquéritos sobre Dietas , Comportamento Alimentar , Feminino , Humanos , Masculino , Estado Nutricional , Estudos Prospectivos
19.
Angew Chem Int Ed Engl ; 60(32): 17564-17569, 2021 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-34050591

RESUMO

Facet-selective nanostructures in living systems usually exhibit outstanding optical and enzymatic properties, playing important roles in photonics, matter exchange, and biocatalysis. Bioinspired construction of facet-selective nanostructures offers great opportunities for sophisticated nanomaterials, but remains a formidable task. We have developed a macromolecule-mediated strategy for the assembly of upconversion nanoparticles (UCNPs)/two-dimensional metal-organic frameworks (2DMOFs) heterostructures with facet selectivity. Both experimental and theoretical results demonstrate that polyvinylpyrrolidone (PVP) can be utilized as an interface-selective mediator to further promote the facet-selective assembly of MOFs onto the surface of UCNPs. The UCNPs/2DMOFs nanostructures with facet selectivity display specific optical properties and show great advantages in anti-counterfeiting. Our demonstration of UCNPs/2DMOFs provides a vivid example for the controlled fabrication of facet-selective nanostructures and can promote the development of advanced functional materials for applications in biosensing, energy conversion, and information assurance.

20.
Mol Med Rep ; 24(1)2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33955500

RESUMO

The acupuncture penetrating line of Baihui (GV20) to Qubin (GB7) spans the parietal, frontal and temporal lobes. The present study aimed to elucidate the mechanism by which electroacupuncture (EA) at GV20­GB7 regulates mitophagy in intracerebral hemorrhage (ICH) and whether it serves a neuroprotective role. A whole blood­induced ICH model was used. Mitophagy­regulating proteins, including BCL/adenovirus E1B 19 kDa­interacting protein 3 (BNIP3), PTEN­induced putative kinase 1 (PINK1), Parkin and apoptosis­associated proteins were detected by western blotting; autophagy following ICH was evaluated by immunofluorescent techniques; morphological characteristics of mitophagy were observed using transmission electron microscopy; and TUNEL assay was performed to determine the number of apoptotic cells. Immunohistochemistry was used to detect p53 expression. The protective role of EA (GV20­GB7) via enhanced mitophagy and suppressed apoptosis in ICH was further confirmed by decreased modified neurological severity score. The results showed that EA (GV20­GB7) treatment upregulated mitochondrial autophagy following ICH and inhibited apoptotic cell death. The mechanism underlying EA (GV20­GB7) treatment may involve inhibition of p53, an overlapping protein of autophagy and apoptosis. EA (GV20­GB7) treatment decreased neurobehavioral deficits following ICH but pretreatment with 3­methyladenine counteracted the beneficial effects of EA (GV20­GB7) treatment. In conclusion, EA (GV20­GB7) improved recovery from ICH by regulating the balance between mitophagy and apoptosis.


Assuntos
Hemorragia Cerebral/terapia , Eletroacupuntura/métodos , Pontos de Acupuntura , Animais , Apoptose/fisiologia , Autofagia/fisiologia , Caspase 3/metabolismo , Hemorragia Cerebral/patologia , Modelos Animais de Doenças , Masculino , Proteínas de Membrana/metabolismo , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Mitofagia/fisiologia , Proteínas Quinases/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos Sprague-Dawley , Índice de Gravidade de Doença , Proteína Supressora de Tumor p53/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Proteína X Associada a bcl-2/metabolismo
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