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1.
Exp Eye Res ; 212: 108786, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34624334

RESUMO

PURPOSE: The purpose of this study is to examine the effect of short-wavelength blue light (SWBL) on cultured human lens epithelial cells (hLECs). The pathogenesis of cataracts after SWBL exposure is discussed. METHODS: HLE-B3 hLECs were randomly divided into 3 groups: the NC group, which was grown in a dark incubator; the acetyl (Ac)-Tyr-Val-Ala-Asp-chloromethyl ketone (AC-YVAD-CMK) treatment group; and the SWBL exposure group. After SWBL (2500 lux) irradiation (for 8, 16, 24, and 32 h), caspase-1 and gasdermin D (GSDMD) expression levels in HLE-B3 hLECs were examined using ELISA, immunofluorescence staining, and Western blotting analyses. Double-positive staining of hLECs for activated and inhibited caspase-1 was used to determine pyroptosis in HLE-B3 hLECs. RESULTS: SWBL led to hLEC death, but a caspase-1 inhibitor suppressed cell death. The flow cytometry results also confirmed the dose-dependent effect of SWBL irradiation on the pyroptotic death of hLECs. Caspase-1 and GSDMD expression levels in all hLEC groups changed with blue light exposure times (8, 16, 24, and 32 h) and were higher in the AC-YVAD-CMK and SWBL exposure groups than in the NC group. The immunofluorescence results revealed higher GSDMD-N expression in the cell membrane of both the AC-YVAD-CMK and SWBL exposure groups than in the NC group. CONCLUSIONS: Based on the data, SWBL induces pyroptotic programmed cell death by activating the GSDMD signalling axis in HLE-B3 hLECs. These results provide new insights into the exploitation of new candidates for the prevention of cataracts.

2.
J Hazard Mater ; 424(Pt A): 127311, 2021 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-34600390

RESUMO

Heavy metal emissions from non-ferrous smelting plants have been a rising concern. However, their emission characteristics were still unclear. In this study, the concentrations and gas-particle partition of five major heavy metals (Cu, Pb, As, Cr and Cd) in the flue gas from a typical copper smelting plant were measured. The bi-modal distribution of both particulate matter and heavy metals indicated that the particles in super-micron mode was caused by the mechanical crushing and escaping of raw materials, whereas the formation of submicron mode was due to the evaporation and subsequent condensation of volatile substances. The excellent performance of existing air pollution control devices in the studied smelter could substantially reduce the particulate matter and heavy metal concentrations in the extraction and smelting stages by 99.2%-99.9%. The emission factors of PM2.5, Cu, Pb, As, Cr, and Cd were only 283, 2.49, 0.97, 5.92, 0.28, and 0.06 g/t, mostly as the fugitive emission (84.2% on average). In addition, the 'unfilterable' phase of the heavy metals, including the gaseous species and solutes in the filter-penetrated droplet, accounted for averagely 45.8% of the total emissions at the outlet, which indicates the huge underestimation by particle collection only.

3.
Nat Med ; 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34663987

RESUMO

Genes involved in distinct diabetes types suggest shared disease mechanisms. Here we show that One Cut Homeobox 1 (ONECUT1) mutations cause monogenic recessive syndromic diabetes in two unrelated patients, characterized by intrauterine growth retardation, pancreas hypoplasia and gallbladder agenesis/hypoplasia, and early-onset diabetes in heterozygous relatives. Heterozygous carriers of rare coding variants of ONECUT1 define a distinctive subgroup of diabetic patients with early-onset, nonautoimmune diabetes, who respond well to diabetes treatment. In addition, common regulatory ONECUT1 variants are associated with multifactorial type 2 diabetes. Directed differentiation of human pluripotent stem cells revealed that loss of ONECUT1 impairs pancreatic progenitor formation and a subsequent endocrine program. Loss of ONECUT1 altered transcription factor binding and enhancer activity and NKX2.2/NKX6.1 expression in pancreatic progenitor cells. Collectively, we demonstrate that ONECUT1 controls a transcriptional and epigenetic machinery regulating endocrine development, involved in a spectrum of diabetes, encompassing monogenic (recessive and dominant) as well as multifactorial inheritance. Our findings highlight the broad contribution of ONECUT1 in diabetes pathogenesis, marking an important step toward precision diabetes medicine.

4.
Environ Sci Technol ; 55(20): 13687-13696, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34618434

RESUMO

Coal preparation is effective in controlling primary mercury emissions in coal combustion systems; however, the combustion of coal preparation byproducts may cause secondary emissions. The inconsistent coal preparation statistics, unclear mercury distribution characteristics during coal preparation, and limited information regarding the byproduct utilization pathways lead to great uncertainty in the evaluation of the effect of coal preparation in China. This study elucidated the mercury distribution in coal preparation based on the activity levels of 2886 coal preparation plants, coal mercury content database, tested mercury distribution factors of typical plants, and then traced the mercury flows and emissions in the downstream sectors using a cross-industry mercury flow model. We found that coal preparation altered the mercury flows by reducing 68 tonnes of mercury to sectors such as coking and increasing the flows to byproduct utilization sectors. Combusting cleaned coal rather than raw coal reduced the mercury emissions by 47 tonnes; however, this was offset by secondary mercury emissions. Coal gangue spontaneous combustion and the cement kiln coprocessing process were dominant secondary emitters. Our results highlight the necessity of whole-process emission control of atmospheric mercury based on flow maps. Future comprehensive utilization of wastes in China should fully evaluate the potential secondary mercury emissions.

5.
J Clin Lab Anal ; : e23995, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34498313

RESUMO

BACKGROUND: Renal biopsy remains the golden standard for diagnosing and monitoring IgA nephropathy (IgAN). Vascular endothelial growth factor A (VEGFA) was crucial for the survival of glomerular cells. Our aim was to screen the expression pattern of urinary, circulating and renal VEGFA in IgAN patients to reveal their relationship with renal pathology and outcomes. METHODS: Baseline VEGFA levels were determined with ELISA, real-time PCR and immunohistochemistry. Associations between VEGFA expression and clinical-pathological parameters, and renal outcomes were evaluated. RESULTS: Compared with healthy controls, urinary VEGFA level was obviously elevated in IgAN patients (76.19 ± 63.67 pg/mg Cr vs 146.67 ± 232.71 pg/mg Cr, p = 0.0291) and not correlated with serum VEGFA level. Baseline urinary VEGFA was significantly associated with gender and tubular atrophy/interstitial fibrosis by stepwise multivariate regression analysis. Urinary VEGFA was higher in male patients accompanied with higher serum creatinine, larger proportion of hypertension and recurrent hematuria than in female patients. In the kidney of IgAN patients, VEGFA were robustly expressed in the parietal epithelial cells, podocytes, mesangial cells and tubular epithelial cells. After a follow-up duration of 38.53 ± 27.14 months, IgAN patients with higher urinary VEGFA level were found to have a poorer renal outcome of renal replacement therapy (HR = 1.027, p = 0.037) or composite outcome (HR = 1.023, p = 0.039) after adjusting for confounders. CONCLUSIONS: Increased urinary VEGFA might reflect certain renal pathology and, although not fully specific, still could be served as a valuable noninvasive indicator in predicting renal progression of IgAN.

6.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120362, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34509887

RESUMO

Glutathione (GSH) as an essential biothiol maintains redox homeostasis in human body, the aberrant level of it has been related to various diseases. In this work, we constructed a facile and environment-friendly strategy by using Ce based metal-organic frameworks and gold nanoparticles (AuNPs) for detection of GSH. The fluorescence intensity of the Ce-MOF was quenched by AuNPs, which is ascribed to the existence of fluorescence resonance energy transfer (FRET) and electrostatic interaction between Ce-MOFs and AuNPS. Because of the formation of Au-SH between AuNPs and GSH, the addition GSH induced the Ce-MOF/AuNPs and prevented the occurrence of FRET and electrostatic interaction between Ce-MOFs and AuNPS, which futher recovered the fluorescence of Ce-MOF. Under the optimized conditions, this "turn-on" sensing process revealed a high selectivity toward GSH and displayed good linearity in range of 0.2-32.5 µM with low detection limit of 58 nM. In addition, the practicability of the strategy was testified through analyzing GSH in real human serum samples.

7.
Environ Sci Technol ; 55(20): 13860-13868, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34590832

RESUMO

This study developed an up-to-date and point-source-based inventory of mercury (Hg) releases to water in China by applying probabilistic release factors that combined industry removal efficiencies, reuse of reclaimed water, and receiving water types. In 2017, the national mercury release to water was estimated to be 50 (35-66) tons, in which 47%, 8%, 7%, and 25% were from nonferrous metal smelting, vinyl chloride monomer (VCM) production, coal-fired boilers, and domestic sewage, respectively. Approximately 95% of mercury was released to inland rivers, and the rest was discharged to lakes or coastal water. The significant sources were identified based on their mercury releases to water. The control of mercury release to water in China shall focus on zinc smelting plants, municipal sewage treatment plants, and the VCM production process. For zinc smelting plants, China can tighten the limit of mercury concentration in discharged wastewater and combine Hg-catcher device in traditional integrated treatment. For municipal sewage treatment plants and the VCM production process, promoting processes of Hg-free production can reduce mercury inputs at the source. Our study provides insights for other parties to identify the relevant sources of mercury release to water and to conduct control measures, so as to promote the global convention implementation.

8.
Talanta ; 234: 122612, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364422

RESUMO

In this work, a ratiometric fluorometric method based on luminol-Europium complex (luminol-Eu) was constructed for the detection of tetracycline (TC). Luminol-Eu, synthesized by self-assembly reaction, displayed a strongly emission peak at 453 nm under excitation at 360 nm which was derived from the aggregation-induced emission (AIE) of the luminol-Eu. In the present of TC, the fluorescence of luminol-Eu at 453 nm was quenched based on inner filter effect (IFE). Meantime, the characteristic emission peak of Eu3+ at 626 nm can be observed thank to antenna effect (AE). Therefore, we proposed a ratiometric fluorometric method for detection of TC, which allowed detection of TC from 0.5 to 80 µM with the detection limit of 39 nM. In addition, the luminol-Eu-based test paper was prepared for visual semi-quantitative detection of TC in real samples based on the color of luminol-Eu change from blue to red under 365 nm ultraviolet light. All of those results indicated that the ratiometric fluorometric strategy was fast, sensitive, and visual for detection of TC.


Assuntos
Corantes Fluorescentes , Luminol , Humanos , Limite de Detecção , Espectrometria de Fluorescência , Tetraciclina
9.
Sci Total Environ ; 798: 149116, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34333439

RESUMO

The ultra-low emission retrofitting (ULE) in China's coal-fired power plants (CFPPs) enhances removal efficiencies of trace elements, which may increase their contents in fly ash and gypsum. However, their potential environmental risks in these wastes have been scarcely evaluated. Experiments indicated that the trace elements in fly ash and gypsum accounted for approximately 92.9-98.2% of the total outputs. Most trace elements in these wastes existed mainly as mobile/leachable forms, except for the Hg in fly ash (residual form). We comprehensively evaluated the potential environmental risks of trace elements in fly ash and gypsum from ULE CFPPs in China using a modified risk assessment approach that integrates a trace element enrichment model for waste, and chemical speciation datasets. We found that nationally, trace elements in gypsum represented low levels of potential risk, even after ULE. However, the potential moderate environmental risk of fly ash has aroused attention because of trace element pollution, where Hg and Cd contributed the major risks. The relatively high risks from fly ash are mainly distributed in Guangxi, Hunan and Hebei provinces. The disposal of fly ash in these areas should be given special attention in the future.


Assuntos
Cinza de Carvão , Oligoelementos , Sulfato de Cálcio , China , Carvão Mineral/análise , Cinza de Carvão/análise , Monitoramento Ambiental , Centrais Elétricas , Oligoelementos/análise
10.
BMC Infect Dis ; 21(1): 774, 2021 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-34372792

RESUMO

BACKGROUND: The severity of COVID-19 associates with the clinical decision making and the prognosis of COVID-19 patients, therefore, early identification of patients who are likely to develop severe or critical COVID-19 is critical in clinical practice. The aim of this study was to screen severity-associated markers and construct an assessment model for predicting the severity of COVID-19. METHODS: 172 confirmed COVID-19 patients were enrolled from two designated hospitals in Hangzhou, China. Ordinal logistic regression was used to screen severity-associated markers. Least Absolute Shrinkage and Selection Operator (LASSO) regression was performed for further feature selection. Assessment models were constructed using logistic regression, ridge regression, support vector machine and random forest. The area under the receiver operator characteristic curve (AUROC) was used to evaluate the performance of different models. Internal validation was performed by using bootstrap with 500 re-sampling in the training set, and external validation was performed in the validation set for the four models, respectively. RESULTS: Age, comorbidity, fever, and 18 laboratory markers were associated with the severity of COVID-19 (all P values < 0.05). By LASSO regression, eight markers were included for the assessment model construction. The ridge regression model had the best performance with AUROCs of 0.930 (95% CI, 0.914-0.943) and 0.827 (95% CI, 0.716-0.921) in the internal and external validations, respectively. A risk score, established based on the ridge regression model, had good discrimination in all patients with an AUROC of 0.897 (95% CI 0.845-0.940), and a well-fitted calibration curve. Using the optimal cutoff value of 71, the sensitivity and specificity were 87.1% and 78.1%, respectively. A web-based assessment system was developed based on the risk score. CONCLUSIONS: Eight clinical markers of lactate dehydrogenase, C-reactive protein, albumin, comorbidity, electrolyte disturbance, coagulation function, eosinophil and lymphocyte counts were associated with the severity of COVID-19. An assessment model constructed with these eight markers would help the clinician to evaluate the likelihood of developing severity of COVID-19 at admission and early take measures on clinical treatment.


Assuntos
COVID-19 , Biomarcadores , China/epidemiologia , Humanos , Estudos Retrospectivos , Medição de Risco , SARS-CoV-2
11.
Mol Syst Biol ; 17(6): e10207, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34096681

RESUMO

The ability to switch a gene from off to on and monitor dynamic changes provides a powerful approach for probing gene function and elucidating causal regulatory relationships. Here, we developed and characterized YETI (Yeast Estradiol strains with Titratable Induction), a collection in which > 5,600 yeast genes are engineered for transcriptional inducibility with single-gene precision at their native loci and without plasmids. Each strain contains SGA screening markers and a unique barcode, enabling high-throughput genetics. We characterized YETI using growth phenotyping and BAR-seq screens, and we used a YETI allele to identify the regulon of Rof1, showing that it acts to repress transcription. We observed that strains with inducible essential genes that have low native expression can often grow without inducer. Analysis of data from eukaryotic and prokaryotic systems shows that native expression is a variable that can bias promoter-perturbing screens, including CRISPRi. We engineered a second expression system, Z3 EB42, that gives lower expression than Z3 EV, a feature enabling conditional activation and repression of lowly expressed essential genes that grow without inducer in the YETI library.

12.
Complement Ther Med ; 60: 102756, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34175408

RESUMO

OBJECTIVES: This study aims to investigate the effects of prenatal music therapy on fetal and neonatal status. DESIGN AND SETTING: A systematic review and meta-analysis of randomized controlled trials. INTERVENTIONS: Ovid MEDLINE, Embase, Web of Science, Cochrane Library, and Chinese Biomedical Literature Database were searched for studies investigating the effects of prenatal music therapy. Two independent investigators carried out the literature selection, data analysis, and evidence quality assessment. Eligible studies were qualitatively described and synthesized using meta-analyses. MAIN OUTCOME MEASURES: The outcomes included fetal or neonatal status. RESULTS: After screening the 821 records yielded by the systematic search, we identified nine eligible studies involving 1419 pregnant women. Eight studies were included in the meta-analysis, and three outcomes were synthesized. Compared with no music therapy, prenatal music therapy did not change fetal heart rate (mean difference [95 % CI]: -0.28 [-3.75-3.20] beat/min, P = 0.88, moderate quality), number of fetal movements (mean difference [95 % CI]: 0.50 [-0.79-1.78] time/min, P = 0.45, low quality), or number of accelerations (mean difference [95 % CI]: 0.16 [-0.87-1.19] time/min, P = 0.76, low quality). This result did not change when two studies with a high risk of bias were excluded. Subgroup analysis showed that prenatal music therapy did not change fetal heart rate, number of fetal movements, or number of accelerations in different intervention phases. CONCLUSIONS: Prenatal music therapy might not change fetal and neonatal status. However, more systematic strategies of prenatal music therapy deserve further exploration.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34118005

RESUMO

Natural biocompatible material is promising candidate for selenite sorption from water since it allows to reuse selenium while dispose of waste materials. In this study, innovative wheat bran-hydroxyapatite (HA) composite was prepared by in situ precipitation method. Scanning electronic microscopy (SEM) and X-ray diffraction (XRD) revealed that the hydroxyapatite aggregated in the fiber matrix of the wheat bran. The results of batch sorption experiments including sorption kinetics, isotherms, and the effect of solution pH showed that the sorption of Se(IV) on the wheat bran-HA adsorbent was fast, endothermic, and pH-independent in the range from 5.0 to 7.0. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis suggested that interaction occurred between Se(IV) and the hydroxyl groups on the composite. Column studies showed that wheat bran-HA composite was suitable to be utilized for continuous Se(IV) removal. The wheat bran-HA composite has a potential application for Se(IV) removal in water treatment.

14.
Nutr Metab Cardiovasc Dis ; 31(7): 2081-2088, 2021 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-34039506

RESUMO

BACKGROUND AND AIMS: Previous studies had demonstrated that elevated monocyte count to high-density lipoprotein cholesterol ratio (MHR), a novel marker of inflammation, was associated with higher cardiovascular events and mortality in patients with pre-dialysis chronic kidney disease, diabetes, and coronary heart disease. However, the association between MHR and mortality in patients undergoing peritoneal dialysis (PD) has received little attention. The aim of this study was to investigate the association between MHR and all-cause and cardiovascular mortality in PD patients. METHODS AND RESULTS: In this single center retrospective cohort study, PD patients who had catheter insertion in our PD center from January 1, 2006 to December 31, 2016 were enrolled. All patients were divided into three groups according to the tertiles of baseline MHR levels and followed up until December 31, 2018. The associations of MHR levels with all-cause and cardiovascular mortality were assessed by using Cox proportional hazards models. Of 1584 patients, mean age was 46.02 ± 14.65 years, 60.1% were male, and 24.2% had diabetes. The mean MHR level was 0.39 ± 0.23. During a median follow up time of 45.6 (24.6-71.8) months, 349 patients died, and 181 deaths were caused by cardiovascular disease. After adjusting for confounders, the highest MHR tertile was significantly associated with all-cause and cardiovascular mortality with a hazard ratio of 1.43 (95%CI = 1.06-1.93, P = 0.019), 1.54 (95%CI = 1.01-2.35, P = 0.046), respectively. CONCLUSION: Higher MHR level was an independent risk factor for all-cause and cardiovascular mortality in PD patients.


Assuntos
HDL-Colesterol/sangue , Nefropatias/terapia , Monócitos , Diálise Peritoneal Ambulatorial Contínua/mortalidade , Adulto , Biomarcadores/sangue , Causas de Morte , Feminino , Humanos , Nefropatias/sangue , Nefropatias/diagnóstico , Nefropatias/mortalidade , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Valor Preditivo dos Testes , Estudos Retrospectivos , Medição de Risco , Fatores de Risco , Fatores de Tempo , Resultado do Tratamento
15.
J Clin Lab Anal ; 35(5): e23754, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33813769

RESUMO

BACKGROUND: Muscle-invasive bladder cancer (MIBC) is a heterogeneous disease with varying clinical courses and responses to treatment. To improve the prognosis of patients, it is necessary to understand such heterogeneity. METHODS: We used single-sample gene set enrichment analysis to classify 35 MIBC cases into immunity-high and immunity-low groups. Bioinformatics analyses were conducted to compare the differences between these groups. Eventually, single-cell mass cytometry (CyTOF) was used to compare the characteristics of the immune microenvironment between the patients in the two groups. RESULTS: Compared with patients in the immunity-low group, patients in the immunity-high group had a higher number of tumor-infiltrating immune cells and greater enrichment of gene sets associated with antitumor immune activity. Furthermore, positive immune response-related pathways were more enriched in the immunity-high group. We identified 26 immune cell subsets, including cytotoxic T cells (Tcs), helper T cells (Ths), regulatory T cells (Tregs), B cells, macrophages, natural killer (NK) cells, and dendritic cells (DCs) using CyTOF. Furthermore, there was a higher proportion of CD45+ lymphocytes and enrichment of one Tc subset in the immunity-high group. Additionally, M2 macrophages were highly enriched in the immunity-low group. Finally, there was higher expression of PD-1 and Tim-3 on Tregs as well as a higher proportion of PD-1+ Tregs in the immunity-low group than in the immunity-high group. CONCLUSION: In summary, the immune microenvironments of the immunity-high and immunity-low groups of patients with MIBC are heterogeneous. Specifically, immune suppression was observed in the immune microenvironment of the patients in the immunity-low group.

16.
Int J Food Microbiol ; 346: 109168, 2021 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-33773355

RESUMO

Knowledge of the effects of various strains of acetic acid bacteria (AAB) on sourdough remains limited. In this study, the diversity of microbial taxa in sourdoughs fermented by different starters was assessed and their functional capacity was evaluated via high-throughput metagenomics sequencing. Results showed that Erwinia (29.43%), Pantoea (45.89%), and Enterobacter (9.16%) were predominant in the blank CK treatment. Lactobacillus (91.40%), Saccharomyces (6.13%), as well as the AAB genus Acetobacter (0.61%) were the dominant microbial genera in the sourdoughs started by yeast and a strain of lactic acid bacteria (YL treatment). By contrast, the dominant genera in the sourdoughs started by yeasts and various LAB and AAB strains (YLA treatment) were Komagataeibacter (0.39%) except for the inoculated Lactobacillus (68.37%), Acetobacter (20.17%), and Saccharomyces (8.31%) species. Functional prediction of these changes in microbial community and diversity revealed that various metabolism-related pathways, including alanine, aspartate, and glutamate metabolism (21.95%), as well as amino acid biosynthesis (19.14%), were predominant in the sourdoughs started by yeast and an AAB strain (YA treatment). Moreover, arginine biosynthesis (11.65%) were the dominant pathways in the YL treatment. The fermented dough added with sourdoughs started with yeast + AAB and yeast + AAB + LAB strains had substantially higher contents (more than 48.58% in total) of essential amino acids than the dough added with sourdoughs started with yeast + LAB strain. These results demonstrated that amino acid biosynthesis has a beneficial effect on sourdoughs inoculated with an AAB strain.


Assuntos
Ácido Acético/metabolismo , Bactérias/isolamento & purificação , Biodiversidade , Pão/microbiologia , Lactobacillales/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Fermentação , Microbiologia de Alimentos , Sequenciamento de Nucleotídeos em Larga Escala , Lactobacillales/classificação , Lactobacillales/genética , Lactobacillales/metabolismo , Metagenômica , Triticum/metabolismo , Triticum/microbiologia , Leveduras/classificação , Leveduras/genética , Leveduras/isolamento & purificação
17.
Anal Bioanal Chem ; 413(12): 3281-3290, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33693975

RESUMO

Phosphate (PO43-) plays a major role in aquatic ecosystems and biosystems. Developing a highly sensitive and selective ratiometric fluorescence probe for detection of PO43- is of great significance to the ecological environment and human health. In this work, a novel dual lanthanide metal organic framework was synthesized via hydrothermal reaction based on Tb3+ and Ce3+ as the center metal ions and terephthalic acid as the organic ligand (designated as Tb-Ce-MOFs). The fluorescence of Tb-Ce-MOFs shows emission at 375 nm. In the presence of PO43-, with increased concentration of PO43-, the fluorescence intensity of Tb-Ce-MOFs at 500 nm and 550 nm increased, while the intensity at 375 nm was reduced. Hence, ratiometric fluorescence detecting of PO43- can be achieved by measuring the ratio of fluorescence at 550 nm (FL550) to 375 nm (FL375) in the fluorescent spectra of the Tb-Ce-MOFs. In this sensing approach, the Tb-Ce-MOFs probe exhibits highly sensitive and selective for detection of PO43-. The limit of detection is calculated to be 28 nM and the detection range is 0.1 to 10 µM. In addition, the Tb-Ce-MOFs were used in the detection of PO43- in real samples. We design and synthesize a mixed lanthanide metal organic framework fluorescence probe (Tb-Ce-MOFs) for ratiometric fluorescence for the detection of PO43- based on Tb3+ and Ce3+ as the center metal ions and terephthalic acid as the organic ligand.

18.
BMC Cardiovasc Disord ; 21(1): 97, 2021 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-33593274

RESUMO

BACKGROUND: To investigate the ability of body shape index (ABSI), body roundness index (BRI), waist circumference (WC), body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), and body adiposity index (BAI) for predicting non-adipose cardio-metabolic risk. METHODS: A total of 17,360 Chinese subjects aged 18-95 years old who escaped cardiovascular disease (CVD) or diabetes were recruited in the cross-sectional study. Anthropometric and biochemical parameters were assessed. Receiver operating characteristic curve (ROC) and multinomial logistic regression were conducted to examine the association of anthropometric indicators with cardio-metabolic risk factors. RESULTS: The mean age of subjects were 53.7(13.1) years, 41.6 % were males. The areas under the curve (AUC) demonstrated that WC, BMI, WHR, WHtR and BRI were able to predict high cardio-metabolic risk (AUC > 0.70). Meanwhile, multinomial logistic regression showed BRI was significantly associated with high cardio-metabolic risk (OR 3.27, 95% CI 3.01-3.55). The optimal cut-off values of BRI for high cardio-metabolic risk were (< 60 y: 3.49 vs. ≥60 y: 3.46) in males and (< 60 y: 3.47 vs. ≥60 y: 3.60) in females. CONCLUSIONS: WC, BMI WHR, and WHtR were potential obesity indicators in discriminating high cardio-metabolic risk, while BAI or ABSI was not. Moreover, BRI revealed superior predictive capacity and significant association with accumulated cardio-metabolic risk factors.


Assuntos
Adiposidade , Antropometria , Síndrome Metabólica/epidemiologia , Obesidade/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Fatores de Risco Cardiometabólico , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Síndrome Metabólica/diagnóstico , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Obesidade/diagnóstico , Obesidade/fisiopatologia , Valor Preditivo dos Testes , Medição de Risco , Adulto Jovem
19.
Hum Immunol ; 82(3): 186-192, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33597097

RESUMO

BACKGROUND: Fc receptor-like (FCRL) molecules were considered to play a role in the pathogenesis of certain autoimmune diseases. Nonetheless, the clinical significance of FCRLs in IgA nephropathy (IgAN) remains unclear. OBJECTIVE: This study is aimed at investigating the expression levels of FCRLs molecules in IgAN patients and determining its relevance to disease activity. METHODS: The mRNA expression levels of FCRLs were determined in peripheral blood mononuclear cells (PBMCs) of 42 IgAN patients and 48 healthy controls by quantitative real-time PCR (qRT-PCR). FCRLs proteins expression in B cells of 25 IgAN patients, 14 patients with non-IgAN glomerulonephritis, and 29 healthy controls were detected by Flow cytometry. The Spearman correlation test was used to assess the correlation of FCRLs expression with clinical parameters of IgAN patients. RESULTS: Our results indicated significant down-regulation of FCRL2 and FCRL3 mRNA levels in IgAN patients compared to healthy subjects. Surface protein expression of FCRLs molecules confirmed the qRT-PCR results. But FCRL2 and FCRL3 protein levels did not correlate with clinicopathologic phenotypes of IgAN patients. However, we found a significant positively correlation of FCRL2 and FCRL3 mRNA expression with the core 1 ß1,3-galactosyltransferase (C1GALT1) and its molecular chaperone (Cosmc) mRNA levels in IgAN patients. CONCLUSIONS: FCRL2 and FCRL3 expression levels in IgAN patients are significantly decreased and correlated with CIGALT1 and Cosmc mRNA expression.


Assuntos
Glomerulonefrite por IGA/metabolismo , Leucócitos Mononucleares/metabolismo , Proteínas de Membrana/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores Imunológicos/metabolismo , Adulto , Células Cultivadas , Regulação para Baixo , Feminino , Galactosiltransferases/genética , Galactosiltransferases/metabolismo , Glomerulonefrite por IGA/genética , Humanos , Leucócitos Mononucleares/patologia , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Receptores de Superfície Celular/genética , Receptores Imunológicos/genética , Transcriptoma
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 252: 119493, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33556795

RESUMO

The sensing of phosphate anion (PO43-) is an important subject for human health and environmental monitoring. Herein, a unique ratiometric fluorescent nanoprobe based on postsynthetic modification of metal-organic frameworks (MOF) UiO-66-(COOH)2 with Eu3+ and Ce3+ ions toward PO43- was proposed (designated as Eu/Ce/Uio-66-(COOH)2). The Eu/Ce/Uio-66-(COOH)2 nanoprobe exhibits three emission peaks at 377 nm, 509 nm, and 621 nm with the single excitation wavelength at 250 nm, respectively. The strong coordinating interaction between Ce3+ and O atoms in the PO43- group can result in the fluorescence quenching at 377 nm, while the fluorescence of 621 nm almost remains unchanged. Such a useful phenomenon is exploited for the construction of a ratiometric fluorescence platform for the detection of PO43-. The assay exhibited a good linear response in the 0.3-20 µM concentration range with the detection limit of 0.247 µM. In addition, this ratiometric fluorescent sensing method not only can be applied to read out PO43- concentration in real water samples, but also shows higher sensitivity, easier preparation and sensing procedures than other detection strategies.

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