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1.
ACS Appl Mater Interfaces ; 13(41): 49066-49075, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34613700

RESUMO

Enhancing the reverse intersystem crossing (RISC) process of thermally activated delayed fluorescent (TADF) emitters is an effective approach to realize efficient organic light-emitting diodes (OLEDs) with low efficiency roll-off. In this work, we designed two novel TADF emitters, SAT-DAC and SATX-DAC, via a spiro architecture. Efficient maximum external quantum efficiencies (EQEs) of 22.6 and 20.9% with reduced efficiency roll-off (EQEs of 17.9 and 17.0% at 1000 cd m-2) were achieved via a "two-RISC-channel" strategy. X-ray diffraction shows close donor (D)/acceptor (A) spacing and suitable D/A orientation in crystals of the two emitters favoring both intra- and intermolecular through-space charge transfer (TSCT) processes. Transient photoluminescence decay measurements show that both emitters have two RISC channels leading to kISCT exceeding 106 s-1. These results suggest that the "two-RISC-channel" design can be a novel approach for enhancing performance of TADF emitters, in particular at high excitation densities.

2.
Front Psychol ; 12: 729790, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621224

RESUMO

Exploring the social factors of mental health among older adults has become a hot topic. This study aimed to examine the relationships between internet use, social capital and depressive symptoms in older adults. Our data were derived from a sample of 6,840 respondents aged 60 and over in the 2018 wave of the China Family Panel Studies. The ordinary least square (OLS) regression results showed that both Internet use characteristics (including access, emotional activities, and online time) and social capital components (including contact with adult children and trust) were protective factors for the prevention of depressive symptoms among older adults. The generalized structural equation modeling (GSEM) results displayed that Internet use not only had a negatively direct effect on depressive symptoms, but also generated a negatively indirect effect on depressive symptoms by structural social capital (i.e., contact with adult children), suggesting that structural social capital mediated the above link. Conversely, the indirect effects of internet use on depressive symptoms via cognitive social capital (i.e., interpersonal trust and institutional trust) were significantly positive, indicating that the relationship between Internet use and depressive symptoms was suppressed by cognitive social capital. These findings address the gaps in previous research on older adults' mental health and have practical implications for policy makers.

3.
Oxid Med Cell Longev ; 2021: 3520034, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34659631

RESUMO

Inhibition of human ether-a-go-go-related gene (hERG) potassium channel is responsible for acquired long QT syndromes, which leads to life-threatening cardiac arrhythmia. A multikinase inhibitor, vandetanib, prolongs the progression-free survival time in advanced medullary thyroid cancer. However, vandetanib has been reported to induce significant QT interval prolongation, which limits its clinical application. Some studies have showed that ginsenoside Rg3 decelerated hERG K(+) channel tail current deactivation. Therefore, in this study, we aim to confirm whether ginsenoside Rg3 targeting hERG K(+) channel could be used to reverse the vandetanib-induced QT interval prolongation. Electrocardiogram (ECG) and monophasic action potential (MAP) were recorded using electrophysiology signal sampling and analysis system in Langendorff-perfused rabbit hearts. The current clamp mode of the patch-clamp technique was used to record transmembrane action potential. The whole-cell patch-clamp technique was used to record the hERG K+ current. In Langendorff-perfused hearts, vandetanib prolonged the QT interval in a concentration-dependent manner with an IC50 of 1.96 µmol/L. In human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), vandetanib significantly prolonged the action potential duration at 50%, 70%, and 90% repolarization (APD50, APD70, and APD90). In stable transfected human hERG gene HEK293 cells, vandetanib caused concentrate-dependent inhibition in the step and tail currents of hERG. As expected, ginsenoside Rg3 relieved vandetanib-induced QT interval prolongation in Langendorff-perfused heart and reversed vandetanib-induced APD prolongation in hiPSC-CMs. Furthermore, ginsenoside Rg3 alleviated vandetanib-induced hERG current inhibition and accelerated the process of the channel activation. Ginsenoside Rg3 may be a promising cardioprotective agent against vandetanib-induced QT interval prolongation through targeting hERG channel. These novel findings highlight the therapeutic potential of ginsenoside to prevent vandetanib-induced cardiac arrhythmia.

4.
Front Chem ; 9: 727631, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34422771

RESUMO

Fluorescent sensors that respond to environmental conditions (temperature, pressure, and pH) have attracted widespread attention in recent years. Generally, traditional solid-state fluorescent materials tend to suffer from aggregation-induced quenching (ACQ) and difficulty of film forming, limiting their extensive applications. Therefore, researchers are focusing more and more attention on fluorescent sensors with aggregation-induced emission (AIE) effects. Herein, the article reports an AIE molecule (TPEBZMZ) containing tetraphenylethylene (TPE) and benzimidazole fragments. The fluorescence properties of TPEBZMZ in solution and aggregation states have been investigated, and the luminescence performance and aggregation structures of solid-state TPEBZMZ after force and acid treatments have been explored. The results show obvious AIE and fluorescent sensing properties of TPEBZMZ, presenting force- and acid-induced discolorations. Moreover, the TPEBZMZ-based fluorescent nanofibrous film is fabricated by electrospinning the solution of TPEBZMZ blended with polylactic acid (PLA), which shows a good nanofiber film structure and exhibits reversible acid-induced discoloration property, even with only 0.5 wt% TPEBZMZ. This work provides a simple strategy to achieve stimulus-responsive fluorescent film.

5.
Org Lett ; 23(16): 6200-6205, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34339192

RESUMO

Herein, we report a highly efficient ruthenium-catalyzed peri-selective C(sp2)-H acylmethylation of 1-naphthols with α-carbonyl sulfoxonium ylides by utilizing hydroxyl as a weakly coordinating directing group. This new method imparts good reactivity, excellent chemo- and regioselectivity, and broad functional group tolerance and involves mild reaction conditions. The C-H acylmethylated products can be readily cyclized into fluorescent annulated pyrans by a one-pot process.

6.
Int J Biol Macromol ; 183: 1948-1958, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-34051256

RESUMO

Aflatoxin contamination is one of the most important factors jeopardizing the quality of traditional Chinese health food (TCHF) during storage. Based on our previous work, we investigated the stability of chitosan (CH) films containing turmeric essential oil (TEO) and employed CH-TEO films as inner pouches, then stored them with inoculated Coix seed, nutmeg, and Ziziphi Spinosae Semen (ZSS). We found that the stability of CH-TEO was most affected by high temperature, and these pouches dramatically decreased aflatoxin accumulation and maintained levels of marker components of each TCHF. We found that glycerol tristearat in Coix seed and jujuboside A and spinosin in ZSS were negatively correlated with aflatoxin accumulation. After three months of storage with a CH-TEO pouch, we found little change in marker components contents, but observed that Coix seed had the relative lower sensory characteristics score. In addition, acute and 90-day subchronic toxicity test in Coix seed stored with the largest amount of TEO showed no significant signs of toxicity or treatment-related changes in animals. The present study is the first report on the study of a green, efficient, and low toxicity solution for aflatoxic contamination in TCHF, and provides strong support for its future use.


Assuntos
Aflatoxinas/análise , Quitosana/química , Curcuma/química , Óleos Voláteis/química , Ziziphus/química , Animais , Coix/química , Feminino , Contaminação de Alimentos , Armazenamento de Alimentos , Temperatura Alta , Masculino , Camundongos , Myristica/química , Óleos Vegetais/química , Ratos , Testes de Toxicidade Aguda , Testes de Toxicidade Subcrônica , Triglicerídeos/química
7.
Environ Pollut ; 286: 117249, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33975215

RESUMO

The relationship between heavy metal(loid)s exposure and oxidative stress damage is a matter of research interest. Our study aimed to investigate the distribution patterns of the nine heavy metal(loid)s in blood of pregnant women, including four toxic heavy metal(loid)s [arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg)] and five typical heavy metal(loid)s [manganese (Mn), cobalt (Co), copper (Cu), zinc (Zn), and selenium (Se)] in blood. Blood samples of 348 women were collected and their concentrations in the serum (sr) and blood cells (bc) were measured, as well as serum heme oxygenase-1 (HO-1) (an oxidative stress marker). Total blood (tb) concentrations of these metal(loid)s and serum-to-blood cell concentration ratios (sr/bc) were further calculated. We found Cu mainly accumulated in the serum compared to the blood cells with Cusr/bc = 2.30, whereas Co, Se, and As evenly distributed between these two fractions. Other metal(loid)s mainly concentrated in the blood cells. Cosr, Cusr, Cubc, Mnbc, Znbc, Cdbc, Cotb, Cutb, Mntb, Zntb, Cdtb, and Cusr/bc were negatively associated with serum HO-1, whereas Assr, Asbc, Astb, Znsr/bc, Cdsr/bc, and Hgsr/bc were positively, indicating of their potential toxicity. We concluded that the distribution patterns of blood heavy metal(loid)s, in particular for Cd, Hg and Zn, which either increased in serum or decreased in blood cells, might be associated with elevated serum oxidative stress, should be considered in environmental health assessments.


Assuntos
Arsênio , Metaloides , Metais Pesados , Arsênio/análise , Monitoramento Ambiental , Feminino , Heme Oxigenase-1 , Humanos , Metais Pesados/análise , Gravidez , Gestantes
8.
J Phys Chem B ; 125(18): 4874-4882, 2021 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-33929854

RESUMO

Cetyltrimethyl ammonium bromide (CTAB) is used to decorate the SiC particle surface. The mechanism of the decoration process has been studied by simulation and experimental approaches. Molecular dynamics (MD) simulation finds a bilayer adsorbed structure of CTAB on the SiC particles, which is then verified by Fourier-transform infrared and thermal gravimetric analysis measurements. The MD simulation also finds that the decorative effects of CTAB on the SiC particle surface are related to the surface charge condition of the SiC particles and the concentration of CTAB. The measured zeta potential of the SiC particles shows dependence on the pH condition and the concentration of CTAB. The decorated SiC particles are used to produce composition by the co-deposition technology. With the help of CTAB, SiC particles are successfully incorporated in the deposited layer, where the content of SiC particles is dependent on the concentration of CTAB and the pH of the bath.

9.
Ecotoxicol Environ Saf ; 217: 112228, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33892343

RESUMO

The relationship between maternal mercury (Hg) intake and the risk of spontaneous preterm birth (SPB) remains unclear. We conducted a nested case-control study from a prospective cohort in Shanxi Province, China, to explore their associations. In total, 126 pregnant women with SPB (cases) and 348 controls with term delivery were included. We measured the Hg concentrations in their serum (Hgs) and blood cell (Hgc) fractions and calculated the concentration ratio of Hg in serum to Hg in blood cells (Hgs/c). We found that only the Hgs/c in the case group was slightly higher than that in control group. The OR of Hgs/c associated with SPB risk was 1.57 [95%CI: 0.99-2.46] with adjusting confounders. After stratification by sampling time, the association above was only statistically significant in the first trimester. High Hgs/c may increase the risk of SPB in the first trimester among women with relatively low Hg exposure.


Assuntos
Poluentes Ambientais/sangue , Exposição Materna/estatística & dados numéricos , Mercúrio/sangue , Nascimento Prematuro/sangue , Adulto , Células Sanguíneas , Estudos de Casos e Controles , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Recém-Nascido , Gravidez , Nascimento Prematuro/epidemiologia , Estudos Prospectivos
10.
BMC Chem ; 15(1): 21, 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33781331

RESUMO

Mulberry leaves are used in traditional Chinese medicine and contain numerous active substances that are known to be beneficial for human health. The aim of this study was to investigate the phenolic compositions and antioxidant activities of the leaves from 23 mulberry cultivars. Qualitative LC-ESI-QTOF analysis revealed the presence of 11 phenolic compounds in the free phenolic extracts and 10 phenolic compounds in the bound fractions. Chlorogenic acid and caffeic acid were the major components in the free and bound fractions, respectively. The results revealed that the changguosang cultivar from Taiwan contained the greatest content of phenolic compounds as well as the highest antioxidant activity among the 23 cultivars examined, as determined using three separate antioxidant assays. The isoquercitrin, chlorogenic acid, and rutin contents of the free phenolic extracts displayed significant correlations with the antioxidant activities, while syringic acid and rutin were the main contributors to the antioxidant activities of the bound phenolic fractions. The obtained results demonstrate that mulberry leaves contain a variety of beneficial phenolic substances and may be suitable for further development as a herbal medicine.

11.
J Vis Exp ; (169)2021 03 11.
Artigo em Inglês | MEDLINE | ID: mdl-33779592

RESUMO

An increasing amount of evidence shows that cognitive deficits and movement dysfunctions are not separated. Patients with mild cognitive impairment (MCI) can manifest fine motor disorders of the upper extremities. Handwriting is a complex and unique human activity involving both motor and cognitive coordination. Researchers from western countries have discovered that patients with MCI have abnormal handwriting features. However, no relevant studies have been conducted in the Chinese population. Owing to the cross-culture phenomenon of handwriting, the aim of this study is to find new handwriting tasks to demonstrate the differences in handwriting features between elderly patients with MCI and age-matched healthy individuals.


Assuntos
Transtornos Cognitivos/fisiopatologia , Disfunção Cognitiva/fisiopatologia , Escrita Manual , Destreza Motora/fisiologia , Idoso , Fenômenos Biomecânicos , China , Feminino , Humanos , Masculino , Movimento , Testes Neuropsicológicos
12.
J Obstet Gynaecol Res ; 47(6): 2157-2165, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33765692

RESUMO

AIM: To clarify the association of serum kisspeptin levels in women with polycystic ovary syndrome (PCOS) by meta-analysis. METHODS: Two English databases and two Chinese databases were searched for the relationship between kisspeptin and PCOS published from 2009. After the studies screening according to specific principles, we used STATA 12.0 for meta-analysis. Standardized mean difference (SMD) and its 95% confidence intervals (95% CIs) were used as the effect size and STATA 12.0 software was performed by this meta-analysis. RESULTS: Nine articles were included in the end, with a total of 1282 participants (699 patients and 583 controls). Heterogeneity between studies was statistically significant. Therefore, the random effects model was used to combine the effects. Meta-analysis showed statistically significant differences in serum kisspeptin levels between the PCOS patients and controls (SMD = 0.57, 95% CI [0.32, 0.82]), which indicated that there is a strong association between serum kisspeptin levels and PCOS. The source of high heterogeneity between the inclusion studies (I2  = 73.2%) might be due to the small sample size. The larger variation of kisspeptin concentration might be caused by different diagnosis criteria of PCOS and short half-time period of kisspeptin combined with nonstandard testing process. CONCLUSION: Serum kisspeptin levels in PCOS patients were higher than non-PCOS patients. It is a hint to indicate us that kisspeptin might be an independent biomarker of PCOS patients.


Assuntos
Kisspeptinas , Síndrome do Ovário Policístico , Biomarcadores , Feminino , Humanos
13.
Cell Death Discov ; 7(1): 31, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33568649

RESUMO

Intracellular ion channel inositol 1,4,5-triphosphate receptor (IP3R1) releases Ca2+ from endoplasmic reticulum. The disturbance of IP3R1 is related to several neurodegenerative diseases. This study investigated the mechanism of IP3R1 in myocardial ischemia/reperfusion (MI/R). After MI/R modeling, IP3R1 expression was silenced in myocardium of MI/R rats to explore its role in the concentration of myocardial enzymes, infarct area, Ca2+ level, NLRP3/Caspase-1, and pyroptosis markers and inflammatory factors. The adult rat cardiomyocytes were isolated and cultured to establish hypoxia/reperfusion (H/R) cell model. The expression of IP3R1 was downregulated or ERP44 was overexpressed in H/R-induced cells. Nifedipine D6 was added to H/R-induced cells to block Ca2+ channel or Nigericin was added to activate NLRP3. IP3R1 was highly expressed in myocardium of MI/R rats, and silencing IP3R1 alleviated MI/R injury, reduced Ca2+ overload, inflammation and pyroptosis in MI/R rats, and H/R-induced cells. The binding of ERP44 to IP3R1 inhibited Ca2+ overload, alleviated cardiomyocyte inflammation, and pyroptosis. The increase of intracellular Ca2+ level caused H/R-induced cardiomyocyte pyroptosis through the NLRP3/Caspase-1 pathway. Activation of NLRP3 pathway reversed the protection of IP3R1 inhibition/ERP44 overexpression/Nifedipine D6 on H/R-induced cells. Overall, ERP44 binding to IP3R1 inhibits Ca2+ overload, thus alleviating pyroptosis and MI/R injury.

14.
Ecotoxicol Environ Saf ; 208: 111632, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396152

RESUMO

Titanium (Ti) is commonly used in additives in the form of titanium dioxide (TiO2). However, our understanding of the effect of Ti on reproductive health remains limited. This nested case-control study, performed in a Ti mining exposure field, investigated the association between maternal blood Ti concentration and the risk of low birth weight (LBW), as well as the potential biological mechanism. A total of 45 women who delivered LBW infants (cases) and 352 women with normal birth weight infants (controls) were included. We collected maternal peripheral blood samples in the first or early second trimester to measure Ti concentration in serum (Tisr) and blood cells (Tibc), as well as inflammatory, lipid, and oxidative stress biomarkers thereof. The demographic characteristics of the women included in the study were also obtained. The results showed that the median total blood Ti concentration (Titb) in the case group was significantly higher than that in the control group (134 vs. 129 ng/mL, P = 0.039). A higher Titb level was associated with a greater risk of LBW [odds ratio = 2.62; 95% confidence interval (CI): 1.16-5.90], but no such association was observed for Tisr or Tibc after adjusting for potential confounders. The serum lipid biomarkers TC, TG, and total lipids (TL) were all negatively associated with Tisr and Titb. Serum 8-OHdG was positively associated with Tibc. We concluded that a high Titb during early pregnancy may increase the risk of LBW. Lipid metabolism and oxidative stress may play an important role in the adverse health effects associated with Ti exposure. Thus, our results merit more attention to the probable adverse effects of titanium exposure during pregnancy.


Assuntos
Recém-Nascido de Baixo Peso , Exposição Materna/efeitos adversos , Saúde Reprodutiva , Titânio/toxicidade , Adulto , Estudos de Casos e Controles , China , Feminino , Humanos , Recém-Nascido , Metabolismo dos Lipídeos/efeitos dos fármacos , Razão de Chances , Estresse Oxidativo/efeitos dos fármacos , Gravidez , Titânio/sangue
15.
Ecotoxicol Environ Saf ; 205: 111298, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32950806

RESUMO

Mulberry (Morus atropurpurea) is an economically important woody tree and has great potential for the remediation of heavy metals. To investigate how cadmium accumulates and its detoxification in mulberry, we assessed the physiological and transcriptomic effects of cadmium contamination and as well as its chemical forms and subcellular distribution. Cadmium significantly inhibited mulberry plant growth and primarily accumulated in mulberry roots. Antioxidant enzymes were induced by cadmium in all tissues of mulberry. Subcellular fractionation analyses of cadmium indicated that the majority was compartmentalized in soluble fraction in roots while it mainly located in cell wall in leaves and stems. The greatest amount of the cadmium was integrated with proteins and pectates in all mulberry tissues. RNA-seq transcriptomic analyses of mulberry roots revealed that various metabolic pathways involved in cadmium stress response such as RNA regulation, hormone metabolism, and response to stress, secondary metabolism, as well as signaling, protein metabolism, transport, and cell-wall metabolism. These results will increase our understanding of the molecular mechanisms of cadmium detoxification in mulberry and provide new insights into engineering woody plants for phytoremediation.


Assuntos
Bioacumulação , Cádmio/toxicidade , Morus/efeitos dos fármacos , Poluentes do Solo/toxicidade , Estresse Fisiológico/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos , Biodegradação Ambiental , Cádmio/metabolismo , Perfilação da Expressão Gênica , Morus/crescimento & desenvolvimento , Morus/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Poluentes do Solo/metabolismo , Estresse Fisiológico/genética
16.
Sci Total Environ ; 743: 140432, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32659548

RESUMO

Road dust, contains enhanced amounts of light rare earth elements (LREEs) in rare earth industrial cities, which poses a serious health risk particularly to children. Road dust samples were collected from Baotou, the largest rare earth industrial city in northern China, and sieved into six size fractions. The pollution characteristics of the LREEs (La, Ce, Pr, and Nd) in the size-fractioned road dust were investigated, and the LREEs exposure dose of children via ingestion of road dust was evaluated. The results showed that the finer size fractions of road dust had enhanced REEs concentrations, and the pollution level was found to be elevated with a decrease in particle size. Ce was the most abundant element, with concentrations ranging from 105 to 5420 mg·kg-1, followed by La, Nd and Pr. REEs mining activities had a major contribution to the LREEs in the road dust and crust, among which coal combustion emissions could also be a potential source. The average daily intake dose of LREEs through ingestion of road dust by children ranged from 4.27 × 10-4 to 2.63 × 10-2 mg·kg-1·day-1 and was significantly affected by particle size. Road dust exposure is notably affected by particle size, and there is a serious risk to children of LREEs uptake from fine particles (<100 µm) of road dust.


Assuntos
Poeira/análise , Metais Terras Raras/análise , Criança , China , Cidades , Monitoramento Ambiental , Humanos , Mineração
17.
Sci Rep ; 10(1): 9204, 2020 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-32514129

RESUMO

Through silicon via (TSV) is one of the most important technologies used in three dimension (3D) packaging. The void-free filling of TSV can be achieved by adding additives into the electrolyte bath during the electrodeposition process. This paper focuses on the effects of three types of commercial additives (the suppressor, the leveler and the accelerator) and analyses additives' interaction on electroplating through experimental investigations. The results showed that the suppressor, the leveler and the accelerator all have chemical behaviour of inhibition in different degrees to the copper electroplating. The interaction experiments of additives in pairs indicated that the suppressor absorbed on the cathode surface was gradually displaced by the accelerator as the concentration of the accelerator increased; the accelerator and the leveler presented a competitive adsorption relationship; the suppressor and the leveler had a synergistic effect for electroplating inhibition especially under high potential and low suppressor concentration. Experiments of micro via filling by electrodeposition have been conducted to investigated the effects of singular additive and multiple additives on the filling process of the micro vias.

18.
Sci Total Environ ; 740: 139759, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-32569908

RESUMO

Mining rare earth elements (REEs) can release large amounts of metal(loid)-rich dust, which can pose significant health risks to local residents. However, compared to other types of particulates, toxicity of mining dust has been largely overlooked. To provide experimental evidence on toxicity of REE mine dust, the study assessed the oxidative stress potential and genotoxicity of inhalable particles collected in a REE mining area, and associated toxicological response with source compositions. Both source types (i.e., mine and tailing area) and distances from source (i.e., industrial and residential areas) were considered when selecting the 44 sampling sites. The particle samples contained 2.3-3.5 folds higher concentrations of tested metal(loid)s than background concentrations in soil. Specially, elevated Fe, REEs, Cd, Pb were found. In spite of low cytotoxicity in lung epithelial A549 cells, there was increased cellular ROS production by of particle exposure. Samples with higher mining-originated source contributions (Provenance Index <0.3) had higher cellular ROS production (1.72 fold, 95%CI: 1.66-1.79 fold) than samples with lower mining contributions (1.58 fold, 95%CI: 1.52-1.65 fold). The factors soil (~46%), mine (~22%), and heavy metal (~20%) sources were recognized by source apportionment analysis as the main contributors to cellular ROS production; importantly, mine and heavy metal sources counted more in industrial samples. While samples generated genotoxicity, there were no differences in DNA damage between the location groups of sampling. Collectively, the results indicate that particles in mining areas may cause ROS production and DNA damage in lung cells depending on mine dust. Coupled with the long-range transportation potential of mine dust, safety measures on open pit and dust disposal sites should be adopted.


Assuntos
Metais Pesados/análise , Poluentes do Solo/análise , Dano ao DNA , Poeira/análise , Monitoramento Ambiental , Mineração , Saúde Pública , Espécies Reativas de Oxigênio
19.
Med Sci Monit ; 26: e924215, 2020 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-32470968

RESUMO

BACKGROUND Dysfunction of small conductance calcium activated potassium (SK) channels plays a vital role in atrial arrhythmogenesis. Amiodarone and dronedarone are the most effective class III antiarrhythmic drugs. It is unclear whether the antiarrhythmic effect of amiodarone and dronedarone is related to SK channel inhibition. MATERIAL AND METHODS Tissue samples were obtained from the right atria of 46 patients with normal sinus rhythm and 39 patients with chronic atrial fibrillation. Isolated atrial myocytes were obtained by enzymatic dissociation. KCNN2 (SK2) channels were transiently expressed in human embryonic kidney (HEK)-293 cells. SK currents were recorded using whole-cell conventional patch clamp techniques. RESULTS Amiodarone and dronedarone showed a concentration-dependent inhibitory effect on SK currents (IKAS) in atrial myocytes from normal sinus rhythm patients and chronic atrial fibrillation patients. The suppressed efficacy of dronedarone and amiodarone on IKAS was greater in atrial myocytes from chronic atrial fibrillation patients than that from normal sinus rhythm patients. Furthermore, in patients with chronic atrial fibrillation, the IC50 value was 2.42 µM with dronedarone and 8.03 µM with amiodarone. In HEK-293 cells with transiently transfected SK2 channels, both dronedarone and amiodarone had a dose-dependent inhibitory effect on IKAS. The IC50 value was 1.7 µM with dronedarone and 7.2 µM with amiodarone in cells from patients with chronic atrial fibrillation. Compared to amiodarone, dronedarone is more efficacy to inhibit IKAS and could be a potential intervention for patients with chronic atrial fibrillation. CONCLUSIONS Dronedarone provides a great degree of IKAS inhibition in atrial myocytes from chronic atrial fibrillation than amiodarone. IKAS might be a potential target of amiodarone and dronedarone for the management of chronic atrial fibrillation.


Assuntos
Fibrilação Atrial/tratamento farmacológico , Dronedarona/farmacologia , Canais de Potássio Ativados por Cálcio de Condutância Baixa/efeitos dos fármacos , Adulto , Amiodarona/metabolismo , Amiodarona/farmacologia , Antiarrítmicos/uso terapêutico , Fibrilação Atrial/metabolismo , Cálcio/metabolismo , Dronedarona/metabolismo , Feminino , Células HEK293 , Átrios do Coração/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Canais de Potássio/efeitos dos fármacos , Canais de Potássio/metabolismo , Canais de Potássio Ativados por Cálcio de Condutância Baixa/metabolismo
20.
Int Immunopharmacol ; 82: 106354, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32143008

RESUMO

The 5-hydroxytryptamine (5-HT) receptor is significant for the regulation of mood and memory. However, the role of 5-HT1AR in ß-Amyloid protein (Aß)-induced cognitive decline, neuroinflammation and the possible mechanism remains elusive. Thus, we aimed to evaluate the effects of 5-HT1AR on Aß-induced learning and memory decline and neuroinflammation in mice. Novel object recognition and Morris water maze tests were performed to observe learning and memory behavior in mice. Protein levels of Iba1, GFAP, MAP2, TNF-α, Tß4, C-fos, IKK-ß, IKB-α, NF-κBp65, phospho-NF-κBp65 in the hippocampus were examined by immunostaining or western blotting. Aß1-42-treatment inducing learning and memory decline was shown in novel object recognition and Morris water maze tests; neuroinflammation shown in immunostaining. Our study found out that 5-HT1AR inhibitor WAY100635 showed significant improvement in Aß-induced learning and memory decline. Moreover, WAY100635 decreases levels of Iba1, GFAP, and TNF-α in the hippocampus, which were related to neuroinflammation. While treatment with 5-HT1AR agonist 8-OH-DPAT or ERK inhibitor U0126 exerted no effects or even aggravated Aß-induced learning and memory decline. In addition, WAY100635 could downregulate phospho-NF-κB in the hippocampus of Aß1-42-injected mice. These results provide new insight into the mechanism, for 5-HT1AR in Aß-induced cognitive impairments through crosstalk with the NF-κB signaling pathway. Our data indicated that WAY100635 was involved in the protective effects against neuroinflammation and improvement of learning and memory in Alzheimer's disease.

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