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1.
Environ Int ; 169: 107522, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36137426

RESUMO

As new agrochemicals are continuously introduced into agricultural systems, it is essential to investigate their uptake and metabolism by plants to better evaluate their fate and accumulation in crops and the subsequent risks to human exposure. In this study, the uptake and elimination kinetics and transformation of a novel insecticide, cyetpyrafen, in two model crops (lettuce and rice) were first evaluated by hydroponic experiments. Cyetpyrafen was rapidly taken up by plant roots and reached a steady state within 24 h, and it was preferentially accumulated in root parts with root concentration factors up to 2670 mL/g. An uptake mechanism study suggested that root uptake of cyetpyrafen was likely to be dominated by passive diffusion and was difficult to transport via xylem and phloem. Ten phase I and three phase II metabolites of cyetpyrafen were tentatively identified in the hydroponic-plant system through a nontarget screening strategy. The structures of two main metabolites (M-309 and M-391) were confirmed by synthesized standards. The metabolic pathways were proposed including hydroxylation, hydrolysis, dehydrogenation, dehydration and conjugation, which were assumed to be regulated by cytochrome P450, carboxylesterase, glycosyltransferase, glutathione S-transferases and peroxidase. Cyetpyrafen and its main metabolites (M-409, M-309 and M-391) were estimated to be harmful/toxic toward nontarget organisms by theoretical calculation. The high bioaccumulation and extensive transformation of cyetpyrafen highlighted the necessity for systematically assessing the crop uptake and metabolism of new agrochemicals.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36141568

RESUMO

Since the 1970s, although the Chinese central government has constructed farmland rights confirmation policy to stimulate the vitality of rural land elements, it is rare to discuss the effectiveness of the policy from the perspective of farmers. This paper applies the deep interview and questionnaire to present an analysis framework consisting of "policy implementation-input behavior in agricultural production-farmland transfer" and testifies the framework with the mediation model. The findings show that, in general, the farmland rights confirmation policy (FRCP) has a significant negative impact on leasing out farmland and a significant positive impact on leasing in farmland. In particular, for farmland leasing out, the mediating effects of agricultural capital input and agricultural time input account for 15.504% and 14.536% of the total effect, respectively. In addition, for leasing farmland in, these two mediating effects accordingly account for 13.798% and 12.155% of the total effect. It is worth noting that in the future, we should understand FRCP based on the consideration of farmers' behavior in a given local context, while also focusing on policy implementation as well as policy design.

3.
Front Microbiol ; 13: 977187, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36060752

RESUMO

Type 2 diabetes mellitus (T2DM) and T2DM-related complications [such as retinopathy, nephropathy, and cardiovascular diseases (CVDs)] are the most prevalent metabolic diseases. Intriguingly, overwhelming findings have shown a strong association of the gut microbiome with the etiology of these diseases, including the role of aberrant gut bacterial metabolites, increased intestinal permeability, and pathogenic immune function affecting host metabolism. Thus, deciphering the specific microbiota, metabolites, and the related mechanisms to T2DM-related complications by combined analyses of metagenomics and metabolomics data can lead to an innovative strategy for the treatment of these diseases. Accordingly, this review highlights the advanced knowledge about the characteristics of the gut microbiota in T2DM-related complications and how it can be associated with the pathogenesis of these diseases. Also, recent studies providing a new perspective on microbiota-targeted therapies are included.

4.
Emerg Microbes Infect ; : 1-75, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069511

RESUMO

Knowing vaccine effectiveness (VE) against variants of concern (VOCs) in the real-world setting is essential for public health decision-making. A systematic landscape of the VE against a series of clinical outcomes caused by the VOCs in the real-world setting is needed. We systematically searched for studies that evaluated VE against VOCs in the real-world setting and collected individual data. We identified 113 studies meeting the eligibility criteria. We found full vaccination provided strong protection against each clinical outcome with summary VE ranging from 86.8% to 96.0% Alpha, moderate protection against infection caused by Beta, Gamma and Delta with summary VE ranging from 70.9% to 72.8%, strong protection against severe disease caused by Delta with summary VE ranging from 84.9% to 90.3%, limited protection with summary VE of 23.5% (95% CI, 17.0-29.5) against infection and moderate protection with summary VE ranging from 56.5% to 82.4% against severe diseases caused by Omicron. Booster vaccination can provide a substantial improvement in protection against Delta and Omicron, but not as much as the Delta. The meta-regression analysis showed that the VE against the Omicron wanned over time, and the VE against hospitalization declined relatively slowly compared to against infection. Those findings supported the need for public health measures, increasing booster vaccination coverage in response to current and new infectious waves driven by variants and developing broadly protective vaccines to confront virus evolution.

5.
Nanomaterials (Basel) ; 12(17)2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36080046

RESUMO

We theoretically propose and experimentally realize a new configuration of a photonic Chern topological insulator (PCTI) composed of a two-dimensional square-hexagon lattice gyromagnetic photonic crystal immersed in an external magnetic field. This PCTI possesses five distinct types of edges and all of them allowed the propagation of truly one-way edge states. We proceeded to utilize this special PCTI to design topological transmission lines of various configurations with sharp turns. Although the wave impedances of the edge states on both sides of the intersections in these transmission lines were very different, definitely no back reflection occurred and no mode-mixing problems and impedance-mismatching issues at the intersections were present, leading to topological resistance-free one-way transport in the whole transmission line network. Our results enrich the geometric and physical means and infrastructure to construct one-way transport and bring about novel platforms for developing topology-driven resistance-free photonic devices.

6.
Front Oncol ; 12: 940314, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35992785

RESUMO

Breast cancer (BC) remains the most prevalent malignancy due to its incidence rate, recurrence, and metastasis in women. Conventional strategies of cancer detection- mammography and tissue biopsy lack the capacity to detect the complete cancer genomic landscape. Besides, they often give false- positive or negative results. The presence of this and other disadvantages such as invasiveness, high-cost, and side effects necessitates developing new strategies to overcome the BC burden. Liquid biopsy (LB) has been brought to the fore owing to its early detection, screening, prognosis, simplicity of the technique, and efficient monitoring. Remarkably, microRNAs (miRNAs)- gene expression regulators seem to play a major role as biomarkers detected in the samples of LB. Particularly, miR-21 and miR-155 among other possible candidates seem to serve as favorable biomarkers in the diagnosis and prognosis of BC. Hence, this review will assess the potential utility of miRNAs as biomarkers and will highlight certain promising candidates for the LB approach in the diagnosis and management of BC that may optimize the patient outcome.

7.
Research (Wash D C) ; 2022: 9871729, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35935139

RESUMO

Intense ultrabroadband laser source of high pulse energy has attracted more and more attention in physics, chemistry, biology, material science, and other disciplines. We report design and realization of a chirped periodically poled lithium niobate nonlinear crystal that supports ultrabroadband second-harmonic generation covering 350-850 nm by implementing simultaneously up to 12 orders of quasiphase matching against ultrabroadband pump laser covering 700-1700 nm with an average high conversion efficiency of about 25.8%. We obtain a flat supercontinuum spectrum with a 10 dB bandwidth covering more than one octave (about 375-1200 nm) and 20 dB bandwidth covering more than two octaves (about 350-1500 nm) in the ultraviolet-visible-infrared regime and having intense energy as 0.17 mJ per pulse through synergic action of second-order and third-order nonlinearity under pump of 0.48 mJ per pulse Ti:sapphire femtosecond laser. This scheme would provide a promising method for the construction of supercontinuum laser source with extremely broad bandwidth, large pulse energy, and high peak power for a variety of basic science and high technology applications.

8.
Environ Pollut ; 311: 119782, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35934153

RESUMO

Air pollution and extreme heat have been responsible for more than a million deaths in China every year, especially in densely urbanized regions. While previous studies intensively evaluated air pollution episodes and extreme heat events, a limited number of studies comprehensively assessed atmospheric hot-and-polluted-episodes (HPE) - an episode with simultaneously high levels of air pollution and temperature - which have potential adverse synergic impacts on human health. This study focused on the Pearl River Delta (PRD) region of China due to its high temperature in summer and poor air quality throughout a year. We employed geostatistical downscaling to model meteorology at a spatial resolution of 1 km, and applied a machine learning algorithm (XGBoost) to estimate a high-resolution (1 km) daily concentration of particulate matter with an aerodynamic diameter ≤2.5 µm (PM2.5) and ozone (O3) for June to October over 20 years (2000-2019). Our results indicate an increasing trend (∼50%) in the frequency of HPE occurrence in the first decade (2000-2010). Conversely, the annual frequency of HPE occurrence reduced (16.7%), but its intensity increased during the second decade (2010-2019). The northern cities in the PRD region had higher levels of PM2.5 and O3 than their southern counterparts. During HPEs, regional daily PM2.5 exceeded the World Health Organization (WHO) and Chinese guideline levels by 75% and 25%, respectively, while the O3 exceeded the WHO O3 standard by up to 69%. Overall, 567,063 (95% confidence interval (CI): 510,357-623,770) and 52,231 (95%CI: 26,116-78,346) excessive deaths were respectively attributable to exposure to PM2.5 and O3 in the PRD region. Our findings imply the necessity and urgency to formulate co-benefit policies to mitigate the region's air pollution and heat problems.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Ozônio , Poluentes Atmosféricos/análise , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , China/epidemiologia , Cidades , Monitoramento Ambiental/métodos , Humanos , Ozônio/análise , Material Particulado/análise , Saúde Pública
9.
Expert Rev Med Devices ; 19(7): 549-560, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35993248

RESUMO

INTRODUCTION: With the widespread availability of portable electrocardiogram (ECG) devices, there will be a surge in ECG diagnoses. Traditional computer-aided diagnosis of arrhythmia mainly relies on the rules of medical knowledge, which are insufficient due to the limitations of data quality and human expert knowledge. The research of arrhythmia detection methods based on artificial intelligence (AI) techniques can assist physicians in high-precision arrhythmia diagnosis. AI algorithms can also be embedded in smart ECG devices to help more people perform early screening for arrhythmia. AREAS COVERED: The primary objective of this paper is to describe the application of AI methods in the process of arrhythmia detection. Meanwhile, the advantages and limitations of various approaches in different applications are summarized to provide guidance and reference for future research work. EXPERT OPINION: Machine learning (ML) and deep learning (DL) algorithms can be more effectively employed to handle ECG signal denoising and quality assessment, wave detection and delineation, and arrhythmia classification problems. The DL approach can automatically learn deep representation features and temporal features of the ECG signal for heartbeat or rhythm classification. The application of AI methods for arrhythmia detection systems will significantly relieve the pressure on physicians to analyze ECGs.


Assuntos
Inteligência Artificial , Eletrocardiografia , Algoritmos , Arritmias Cardíacas/diagnóstico , Frequência Cardíaca , Humanos
10.
Nanomaterials (Basel) ; 12(16)2022 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-36014742

RESUMO

Carbon nanotube-reinforced magnesium matrix (CNTs/Mg) composite has great application potential in the transportation industry, but the trade-off between strength and ductility inhibits its widespread application. In order to balance the strength and plasticity of the composite, in this work, on the basis of the AZ61 matrix composite homogeneously reinforced by Ni-coated CNTs (hard phase), 30 vol.% large-size AZ61 particles are introduced as an isolated soft phase to fabricate hierarchical CNTs/AZ61 composites. The compression tests show the fracture strain and compressive strength of this composite increases by 54% and 8%, respectively, compared with homogeneous CNTs/AZ61 composite. During deformation, the hard phase is mainly responsible for bearing the load and bringing high strength, due to the precipitation of the Mg17Al12 phase, uniformly dispersed CNT and strong interfacial bonding of the CNTs/Mg interface through nickel plating and interfacial chemical reaction. Furthermore, the toughening of the soft phase results in high ductility. With the increase in CNT content, the compressive strength of composites is nearly unchanged but the fracture strain gradually decreases due to the stress concentration of CNT and its agglomeration.

11.
Front Endocrinol (Lausanne) ; 13: 974042, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35992132

RESUMO

The objective of this systematic review is to synthesize the available evidence on the effectiveness of magnesium supplements on the markers of inflammation, oxidative stress (OS), and metabolism in PCOS patients and to provide a basis for its clinical treatment. Electronic databases (PubMed, Cochrane Library databases, Embase, Web of science, CMB, CNKI, VIP, Wan Fang and ClinicalTrials.gov) were searched from their inception until January 2022. Randomized controlled trials (RCTs) for PCOS undergoing therapy with magnesium supplementation alone or in combination with other agents. The primary outcomes were the markers of blood glucose and OS.363 patients from nine RCTs were included in the current systematic review. Four of the nine studies reported the effects of magnesium supplementation alone on OS or metabolic markers in women with PCOS. Whilemagnesium supplementation alone did not show any significant improvement in the markers of inflammation, OS or metabolism in PCOS, seven of the nine articles reported the effect of magnesium co-supplementation on OS or metabolic markers in PCOS patients. Magnesium combined with vitamin E or zinc-calcium-vitamin D significantly improved glucose and lipid metabolism in PCOS patients. Magnesium intake alone did not lead to a significant improvement in the markers of OS, blood glucose, or serum lipids in PCOS. However, magnesium combined with other supplements (vitamin E, zinc, zinc-calcium-vitamin D) significantly improved serum hs-CRP, insulin, HOMA-IR, TG, TC levels, and the improvement in OS markers was inconclusive. The effect of magnesium and melatonin supplementation on the markers of metabolism needs to be further verified. System Review Registration: PROSPERO https://www.crd.york.ac.uk/PROSPERO/#myprospero, CRD42022303410.


Assuntos
Síndrome do Ovário Policístico , Biomarcadores , Glicemia/metabolismo , Cálcio/metabolismo , Suplementos Nutricionais , Feminino , Humanos , Inflamação/tratamento farmacológico , Magnésio , Estresse Oxidativo , Vitamina D , Vitamina E/farmacologia , Vitamina E/uso terapêutico , Zinco
12.
Medicine (Baltimore) ; 101(31): e29561, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35945780

RESUMO

BACKGROUND: Renal cell carcinoma (RCC) is a malignant tumor of urinary system, and clear cell RCC (ccRCC) is the major pathological subtype. A high-frequency mutation in SETD2 gene is related to the occurrence, development, and poor prognosis of RCC. OBJECTIVE: The research of immune-related genes (IRGs) is important to the success of immunotherapy in RCC. The aim of this study was to develop SETD2-related immune prognostic signature (IPS) potentially useful in the prognosis prediction of ccRCC. METHODS: The expression profile, mutation profile, and clinical data related to ccRCC were obtained from the TCGA (Cancer Genome Atlas) and cBioPortal databases. The data of IRGs were downloaded from the ImmPort database. RESULTS: An IPS with 5 genes (PDIA2, PAEP, AMELX, GREM2, and INHA) was constructed by analyzing the correlation between prognosis data and IRGs associated with ccRCC patients with wild type and mutant SETD2 genes. The clinical utility of the IPS and its relationship with immune microenvironment were also studied. CONCLUSIONS: According to the results of this study, the IPS can be a promising biomarker of ccRCC to guide its prognosis and treatment.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Carcinoma de Células Renais/patologia , Humanos , Neoplasias Renais/patologia , Mutação , Prognóstico , Microambiente Tumoral
13.
Plant Foods Hum Nutr ; 2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-35986175

RESUMO

Recent epidemiologic studies have demonstrated a link between the consumption of daily functional fruits rich in phenols and the prevention of disease for neurodegenerative disorders. Hawthorn products are derived from the functional fruit hawthorn, which is rich in phenols and has been used around the world for centuries. In order to explore the phenolic components in hawthorn, the investigation of the ethanol extract led to the separation of five new phenol compounds (1a/1b, 2-4), including one pair of enantiomers (1a/1b), along with seven disclosed analogs (5-11). Their structures were elucidated based on extensive spectroscopic analyses and electronic circular dichroism (ECD). The compounds (1-11) were tested for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS), and ferric reducing antioxidant power (FRAP) methods. Apart from that, monomeric compounds 2, 4, and 6 exhibited more potent protective capabilities against H2O2 (hydrogen peroxide)-induced SH-SY5Y cells. Meanwhile, electronic analyses were performed using the highest occupied molecular orbital (HOMO), and the lowest unoccupied molecular orbital (LUMO) to analyze compounds 2, 4, and 6. Furthermore, compounds (1-11) measured acetylcholinesterase (AChE) inhibitory activities, and 2, 4, and 6 possessed greater AChE inhibitory activity than donepezil. At the same time, molecular docking was used to investigate the possible mechanism of the interaction between active compounds (2, 4, and 6) and AChE.

14.
Artigo em Inglês | MEDLINE | ID: mdl-35955021

RESUMO

In this study, the influence of rural labor transfer and its spatial spillover effect on the eco-efficiency of cultivated land use (ECLU) in different regions were investigated using the undesirable super-efficiency epsilon based measure (EBM) and spatial Durbin models and data of 31 Chinese provinces for the period 1990-2018. The results show that: (1) China's rural labor transfer rate increased; (2) in the east region, the ECLU has exceeded the national average level since 2001. In the west and northeast regions, the ECLU was higher, whereas it remained below the national average level in Central China; (3) in the whole country, west, and northeast regions, the effect of rural labor transfer on the ECLU was first negative and then positive, whereas it was insignificant in East and Central China. In Central, West, and Northeast China, the effect of the labor transfer on the ECLU had significant spatial spillover effects; (4) a significant U-shaped trend was observed between the local labor transfer and ECLU in the whole country, west, and northeast regions. A positive linear correlation was determined for Central China; labor transfer in other regions had significant indirect effects on the ECLU in Central and Northeastern China. In conclusion, China's rural labor transfer had a significant spatial spillover effect on the ECLU, and differences were observed between East, Central, West, and Northeast China.


Assuntos
Eficiência , População Rural , China , Desenvolvimento Econômico , Humanos
15.
Angew Chem Int Ed Engl ; : e202210988, 2022 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-35979672

RESUMO

Activation of the cyclic GMP-AMP synthase-stimulator of the interferon gene (cGAS-STING) pathway is a potent anticancer immunotherapeutic strategy, and the induction of pyroptosis is a feasible way to stimulate the anticancer immune responses. Herein, two PtII complexes (Pt1 and Pt2) were designed as photoactivators of the cGAS-STING pathway. In response to light irradiation, Pt1 and Pt2 could damage mitochondrial/nuclear DNA and the nuclear envelope to activate the cGAS-STING pathway, and concurrently induce pyroptosis in cancer cells, which evoked an intense anticancer immune response in vitro and in vivo. Overall, we present the first photoactivator of the cGAS-STING pathway, which may provide an innovative design strategy for anticancer immunotherapy.

16.
Nanomaterials (Basel) ; 12(14)2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35889655

RESUMO

Carbon nanotubes (CNTs) reinforced magnesium matrix composites have great application potential in the transportation industry, but the low absolute strength is the main obstacle to its application. In this paper, copper-coated CNTs and AZ61 powder were used as raw materials to prepare CNTs/refined-AZ61 composites with good interfacial bonding, uniformly dispersed CNTs and fine grains by the process of ball milling refinement of AZ61 powder, ball milling dispersion and hot-pressing sintering. When the volume fraction of CNTs is less than or equal to 1 vol.%, CNTs can be uniformly dispersed and the yield strength and compressive strength of composites increase with higher CNT content. When the volume fraction of CNTs is 1 vol.%, the compressive strength and yield strength of composites reach 439 MPa and 361 MPa, respectively, which are 14% and 9% higher than those of matrix composites with nearly the same value of fracture strain. When the volume fraction of CNTs is greater than 1 vol.%, with the increase in CNT content, CNT clustering becomes more and more serious, resulting in a decrease in the strength and fracture strain of composites.

17.
Nanomaterials (Basel) ; 12(14)2022 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-35889672

RESUMO

We have studied the transmission properties of odd/even one-way modes and their reversible conversion in a double-channel waveguide consisting of two magneto-optical photonic crystals (MOPCs) sandwiched with Al2O3 PC. There exist two pairs of even and odd modes, i.e., M1(even)/M2(odd) or M3(odd)/M4(even) modes, for the double-channel waveguides with one- or two-stranded coupling layer of Al2O3 rods, respectively. Among them, the M1, M2, and M3 modes are caused by the weak coupling strength of two sub-waveguides, while the M4 mode results from the strong coupling effect and supports dispersionless slow-light propagation. Furthermore, we realize the reversible conversion between odd and even modes (i.e., between M1 and M2 modes, or M3 and M4 modes) in the one- or two-stranded structure, respectively, by adjusting the length and position of the perfect electric conductor (PEC) defect properly to cause the desired significant phase delay along the upper and lower equivalent transmission paths. Additionally, we find that the robustness of the M1 even mode is poor because of extra excitations of counter-propagation modes near the right Brillouin boundary, while the other three modes have extremely strong robustness against PEC defects and their one-way transmittances are nearly 100%. These results hold promise for many fields, such as slow-light modulation and the design of topological devices.

18.
Int J Mol Sci ; 23(15)2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35897842

RESUMO

Fluoride is widely distributed, and excessive intake will lead to dental fluorosis. In this study, six offspring rats administrated 100 mg/L sodium fluoride were defined as the dental fluorosis group, and eight offspring rats who received pure water were defined as the control group. Differentially expressed proteins and metabolites extracted from peripheral blood were identified using the liquid chromatography tandem mass spectrometry and gas chromatography mass spectrometry, with the judgment criteria of fold change >1.2 or <0.83 and p < 0.05. A coexpression enrichment analysis using OmicsBean was conducted on the identified proteins and metabolites, and a false discovery rate (FDR) < 0.05 was considered significant. Human Protein Atlas was used to determine the subcellular distribution of hub proteins. The Gene Cards was used to verify results. A total of 123 up-regulated and 46 down-regulated proteins, and 12 up-regulated and 2 down-regulated metabolites were identified. The significant coexpression pathways were the HIF-1 (FDR = 1.86 × 10-3) and glycolysis/gluconeogenesis (FDR = 1.14 × 10-10). The results of validation analysis showed the proteins related to fluorine were mainly enriched in the cytoplasm and extrinsic component of the cytoplasmic side of the plasma membrane. The HIF-1 pathway (FDR = 1.01 × 10-7) was also identified. Therefore, the HIF-1 and glycolysis/gluconeogenesis pathways were significantly correlated with dental fluorosis.


Assuntos
Fluorose Dentária , Animais , Fluoretos , Fluorose Dentária/metabolismo , Gluconeogênese , Glicólise , Humanos , Proteômica/métodos , Ratos , Transdução de Sinais
19.
Cell Death Discov ; 8(1): 304, 2022 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-35781287

RESUMO

BACKGROUND: Non-pathological cognitive decline is a neurodegenerative condition associated with brain aging owing to epigenetic changes, telomere shortening, stem cells exhaustion, or altered differentiation. Human umbilical cord mesenchymal stem cells (hUCMSCs) have shown excellent therapeutic prospects on the hallmarks of aging. In this study, we aimed to elucidate the role of hUCMSCs with down-regulated miRNA-206 (hUCMSCs anti-miR-206) on cognitive decline and the underlying mechanism. METHODS: After daily subcutaneous injection of D-gal (500 mg/kg/d) for 8 weeks, 17-week-old male C57BL/6 J mice were stem cells transplanted by lateral ventricular localization injection. During the 10-day rest period, were tested the behavioral experiments applied to cognitive behavior in the hippocampus. And then, the mice were sacrificed for sampling to complete the molecular and morphological experiments. RESULTS: Our behavioral experiments of open field test (OFT), new object recognition test (NOR), and Y-maze revealed that D-galactose (D-gal)-induced aging mice treated with hUCMSCs anti-miR-206 had no obvious spontaneous activity disorder and had recovery in learning and spatial memory ability compared with the PBS-treated group. The hUCMSCs anti-miR-206 reconstituted neuronal physiological function in the hippocampal regions of the aging mice with an increase of Nissl bodies and the overexpression of Egr-1, BDNF, and PSD-95. CONCLUSION: This study first reports that hUCMSCs anti-miR-206 could provide a novel stem cell-based antiaging therapeutic approach.

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