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1.
Food Chem Toxicol ; : 112696, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34822940

RESUMO

Di-2-ethylhexyl phthalate (DEHP) and Dioctyl phthalate (DnOP) are widely used as plasticizers in various industries for which the consequent health problems are of great concern. In this context, we treated HepG2 cells with DEHP or DnOP for 48 h. The results showed that DEHP and DnOP caused increase in oxygen species (ROS), malondialdehyde (MDA), Alanine aminotransferase (ALT) and Aspartate transaminase (AST). The proteins NF⁃E2-related factor 2 (Nrf2) and haemeoxygenase-1 (HO-1), were significantly down-regulated. Subsequently, the mitochondrial structure was disrupted, and the ATP content, the mitochondrial copy number as well as the expression of the corresponding mitochondrial genes were also reduced. The expression of sirtuin 1(SIRT1), PPAR gamma co-activator 1 alpha (PGC-1α), Nuclear respiratory factor 1(Nrf1), Mitochondrial transcription factor A (TFAM) on the SIRT1/PGC-1α pathway were significantly reduced. Finally, neither DEHP nor DnOP was found to induce apoptosis, but could significantly up-regulate Light chain 3 II (LC3II) levels. In conclusion, DEHP and DnOP could induce HepG2 cell damage via mitochondria, probably by causing oxidative stress, inhibiting the Nrf2 pathway and inhibiting the mitochondrial biogenesis pathway, which leads to excessive autophagy and cell death. DEHP and DnOP differ in the Nrf2 pathway, autophagic pathway and MAPK pathway, which may be structurally related.

2.
Micromachines (Basel) ; 12(10)2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34683300

RESUMO

Microrobots have received great attention due to their great potential in the biomedical field, and there has been extraordinary progress on them in many respects, making it possible to use them in vivo clinically. However, the most important question is how to get microrobots to a given position accurately. Therefore, autonomous actuation technology based on medical imaging has become the solution receiving the most attention considering its low precision and efficiency of manual control. This paper investigates key components of microrobot's autonomous actuation systems, including actuation systems, medical imaging systems, and control systems, hoping to help realize system integration of them. The hardware integration has two situations according to sharing the transmitting equipment or not, with the consideration of interference, efficiency, microrobot's material and structure. Furthermore, system integration of hybrid actuation and multimodal imaging can improve the navigation effect of the microrobot. The software integration needs to consider the characteristics and deficiencies of the existing actuation algorithms, imaging algorithms, and the complex 3D working environment in vivo. Additionally, considering the moving distance in the human body, the autonomous actuation system combined with rapid delivery methods can deliver microrobots to specify position rapidly and precisely.

3.
J Clin Lab Anal ; : e24031, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34716619

RESUMO

BACKGROUND: In this study, we aimed to screen methylation signatures associated with the prognosis of patients with clear cell renal cell carcinoma (ccRCC). METHODS: Gene expression and methylation profiles of ccRCC patients were downloaded from publicly available databases, and differentially expressed genes (DEGs)-differentially methylated genes (DMGs) were obtained. Subsequently, gene set enrichment and transcription factor (TF) regulatory network analyses were performed. In addition, a prognostic model was constructed and the relationship between disease progression and immunity was analyzed. RESULTS: A total of 23 common DEGs-DMGs were analyzed, among which 14 DEGs-DMGs were obtained with a cutoff value of PCC < 0 and p < 0.05. The enrichment analysis showed that the 14 DEGs-DMGs were enriched in three GO terms and three KEGG pathways. In addition, a total of six TFs were shown to be associated with the 14 DEGs-DMGs, including RP58, SOX9, NF-κB65, ATF6, OCT, and IK2. A prognostic model using five optimized DEGs-DMGs which efficiently predicted survival was constructed and validated using the GSE105288 dataset. Additionally, four types of immune cells (NK cells, macrophages, neutrophils, and cancer-associated fibroblasts), as well as ESTIMATE, immune, and stromal scores were found to be significantly correlated with ccRCC progression (normal, primary, and metastasis) in addition to the five optimized DEGs-DMGs. CONCLUSION: A five-gene methylation signature with the predictive ability for ccRCC prognosis was investigated in this study, consisting of CCNB2, CDKN1C, CTSH, E2F2, and ERMP1. In addition, potential targets for methylation-mediated immunotherapy were highlighted.

4.
Contrast Media Mol Imaging ; 2021: 6891120, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34671229

RESUMO

Background: Wrist joint injury refers to the injury of the wrist joint caused by excessive stretching of the ligaments and joint capsules around the joint caused by indirect violence. The tissue structure of the wrist joint is complex, and the clinical diagnosis effect is poor. Methods: The purpose of this study was to improve the diagnostic accuracy of wrist joint injuries and provide evidence for imaging analysis and automatic diagnosis of lesions in patients with wrist joint injuries. The Canny algorithm was adopted to extract the edge features of the patient's magnetic resonance imaging (MRI) image, and the particle swarm optimization-support vector machine (PSO-SVM) algorithm was applied to segment the lesion. The image processing effect of the algorithm was evaluated by taking peak signal to noise ratio (PSNR), mean square error (MSE), figure of merit (FOM), and structural similarity (SSIM) as indicators. The accuracy, sensitivity, specificity, and Dice similarity coefficient of the algorithm were analyzed to evaluate the diagnostic accuracy in WJI. Results: Compared with the Gradient Vector Flo (GVF) algorithm and the Elastic Automatic Region Growing (ERG) algorithm, the edge stability of the PSO-SVM algorithm was stable above 0.9. After the quality of images processed using different algorithms was analyzed, it was found that the PSNR of the PSO-SVM algorithm was 26.891 ± 5.331 dB, the MSE was 0.0014 ± 0.0003, the FOM was 0.8832 ± 0.0957, and the SSIM was 0.9032 ± 0.0807. The four indicators were all much better than those of the GVF algorithm and the EARG algorithm, showing statistically obvious differences (P < 0.05). Analysis on diagnostic accuracy of different algorithms for WJI suggested that the diagnostic accuracy of the PSO-SVM algorithm was 0.9413, the sensitivity was 0.9129, the specificity was 0.9088, and the Dice similarity coefficient was 0.8715. The four indicators all showed statistically great difference compared with those of the GVF algorithm and the EARG algorithm (P < 0.05). Conclusions: The PSO-SVM algorithm showed excellent edge detection performance and higher accuracy in the diagnosis of WJI, which can assist clinicians in the clinical auxiliary diagnosis of WJI.

5.
J Prosthodont Res ; 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34588399

RESUMO

PURPOSE: Although mock-ups have been widely used in dental esthetic rehabilitation, their accuracy has not been quantitatively evaluated, and the methods of fabricating mock-ups are various. This in vitro study investigated the thickness accuracy of mock-ups fabricated with different silicone matrices. METHODS: Mock-ups of maxillary anterior teeth were respectively fabricated by 72 silicone matrices that were equally divided into four groups (n=18 for each group) according to two variables of the silicone matrices: labial margin position (equigingival or cover labial gingiva for 1-2 mm) and palatal notches (with or without notches on the palatal side of silicone matrices). The thickness accuracy of the mock-ups was analyzed using 3D scanning and 3D deviation analysis techniques compared with diagnostic waxing. The thickness change ratios of the mock-ups were compared using a two-way analysis of variance (ANOVA). One-way ANOVA and Kruskal-Wallis tests were used to compare differences in thickness change ratios between different teeth in each group. RESULTS: The thickness accuracy of the mock-ups was significantly affected by the labial margin position and the palatal notches of the silicone matrices, respectively, in the labial area and the incisal area. The most accurate mock-ups were made using silicone matrices with equigingival labial margins and palatal notches. The thickness accuracy of the mock-ups was also inconsistent on different teeth. CONCLUSIONS: The mock-ups fabricated by silicone matrices were thicker than the diagnostic waxing. The application of silicone matrices to equigingival labial margins and palatal notches was beneficial to the thickness accuracy of mock-ups.

6.
Front Immunol ; 12: 673693, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34408744

RESUMO

Background: Thymosin alpha 1 (Tα1) is widely used to treat patients with COVID-19 in China; however, its efficacy remains unclear. This study aimed to explore the efficacy of Tα1 as a COVID-19 therapy. Methods: We performed a multicenter cohort study in five tertiary hospitals in the Hubei province of China between December 2019 and March 2020. The patient non-recovery rate was used as the primary outcome. Results: All crude outcomes, including non-recovery rate (65/306 vs. 290/1,976, p = 0.003), in-hospital mortality rate (62/306 vs. 271/1,976, p = 0.003), intubation rate (31/306 vs. 106/1,976, p = 0.001), acute respiratory distress syndrome (ARDS) incidence (104/306 vs. 499/1,976, p = 0.001), acute kidney injury (AKI) incidence (26/306 vs. 66/1,976, p < 0.001), and length of intensive care unit (ICU) stay (14.9 ± 12.7 vs. 8.7 ± 8.2 days, p < 0.001), were significantly higher in the Tα1 treatment group. After adjusting for confounding factors, Tα1 use was found to be significantly associated with a higher non-recovery rate than non-Tα1 use (OR 1.5, 95% CI 1.1-2.1, p = 0.028). An increased risk of non-recovery rate associated with Tα1 use was observed in the patient subgroups with maximum sequential organ failure assessment (SOFA) scores ≥2 (OR 2.0, 95%CI 1.4-2.9, p = 0.024), a record of ICU admission (OR 5.4, 95%CI 2.1-14.0, p < 0.001), and lower PaO2/FiO2 values (OR 1.9, 95%CI 1.1-3.4, p = 0.046). Furthermore, later initiation of Tα1 use was associated with a higher non-recovery rate. Conclusion: Tα1 use in COVID-19 patients was associated with an increased non-recovery rate, especially in those with greater disease severity.


Assuntos
COVID-19/tratamento farmacológico , Síndrome do Desconforto Respiratório/epidemiologia , Timalfasina/efeitos adversos , Adulto , Idoso , COVID-19/complicações , COVID-19/diagnóstico , COVID-19/mortalidade , Feminino , Mortalidade Hospitalar , Humanos , Unidades de Terapia Intensiva/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Escores de Disfunção Orgânica , Prognóstico , Síndrome do Desconforto Respiratório/etiologia , Síndrome do Desconforto Respiratório/prevenção & controle , Estudos Retrospectivos , Medição de Risco/estatística & dados numéricos , Timalfasina/administração & dosagem , Resultado do Tratamento
7.
Analyst ; 146(15): 4841-4847, 2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34223580

RESUMO

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers [concanavalin A (Con A)-Cu3(PO4)2] were used as the signal probe of the sandwich structure. The inorganic component, the copper ions of hybrid nanoflowers, was first used to amplify signal transduction for enzyme-free detection. Sodium ascorbate could dissolve Cu3(PO4)2 of the signal probe to produce Cu2+, which was subsequently converted to Cu+, triggering the Cu+-catalyzed alkyne-azide cycloaddition (CuAAC) reaction between azide-functionalized ssDNA (a fragment of the DNAzyme-containing sequence) and alkyne-functionalized ssDNA immobilized onto the electrode surface. As a result, the DNAzyme was immobilized onto the gold electrode, which produced a positive and stable electrical signal. An exceptional linear relationship was observed between the electrical signal and the concentration of Salmonella typhimurium (101-107 CFU mL-1) with a detection limit of 10 CFU mL-1. The developed electrochemical biosensor based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzymes exhibited good results in detecting S. typhimurium in milk samples.


Assuntos
Técnicas Biossensoriais , DNA Catalítico , Bactérias , Química Click , Cobre , Técnicas Eletroquímicas , Ouro , Limite de Detecção
8.
Anal Methods ; 13(30): 3379-3385, 2021 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-34235517

RESUMO

Here, we describe a simple, sensitive, and enzyme-free method for visual point-of-care detection of 16S rRNA of Escherichia coli O157:H7 based on an isothermal strand displacement-hybrid chain reaction (ISD-HCR) and lateral flow strip (LFS). In this study, the secondary structure of 16S rRNA of E. coli O157:H7 was unwound by two helper oligonucleotides to expose the single-strand-specific nucleic acid sequence. The free specific sequence promoted the toehold-mediated strand displacement reaction to output a large number of FITC-labeled single-stranded DNA probes (capture probe [CP]). The 3'-end sequence of the reporter probe propagated a chain reaction of hybridization events between the two hairpin probes modified with biotin to form long nicked DNA polymers with multiple biotins (RP-HCR complexes); the free CP and RP-HCR complexes then form CP/RP-HCR complexes. The biotin-labeled double-stranded DNA CP/RP-HCR polymers then introduced numerous streptavidin (SA)-labeled gold nanoparticles (AuNPs) on the LFS. The accumulation of AuNPs produced a characteristic red band, which enabled visual detection of changes in the signal of 16S rRNA of E. coli O157:H7. The current approach could detect E. coli O157:H7 at concentrations as low as 102 CFU mL-1 without instrumentation. This approach thus provides a simple, sensitive, and low-cost tool for point-of-care detection of pathogenic bacteria, especially in resource-limited countries.


Assuntos
Escherichia coli O157 , Nanopartículas Metálicas , Escherichia coli O157/genética , Ouro , RNA Ribossômico 16S/genética , Estreptavidina
9.
Foods ; 10(6)2021 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-34071051

RESUMO

Small quantities of lipids accumulate in the white rice grains. These are grouped into non-starch lipid and starch lipid fractions that affect starch properties through association with starch. Lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) are two major lipid classes in the two fractions. Using high-oleic rice grains, we investigated the fatty-acid composition in flour and starch by LC-MS and evaluated its impact on starch properties. In the wild-type grain, nearly 50% of fatty acids in LPC and LPE were palmitic acid (C16:0), over 20% linoleic acid (C18:2) and less than 10% oleic acid (C18:1). In the high-oleic rice grain, C18:1 increased at the expense of C18:2 and C16:0. The compositional changes in starch lipids suggest that LPC and LPE are transported to an amyloplast with an origin from endoplasmic reticulum-derived PC and PE during endosperm development. The high-dissociation temperature of the amylose-lipid complex (ALC) and restricted starch swelling power in the high-oleic rice starch indicates that the stability of the ALC involving C18:1 is higher than that of C18:2 and C16:0. This study provides insight into the lipid deposition and starch properties of rice grains with optimized fatty-acid composition.

10.
Clin Microbiol Infect ; 27(10): 1488-1493, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34020032

RESUMO

OBJECTIVES: Intravenous immunoglobulin (IVIG) is commonly used to treat severe COVID-19, although the clinical outcome of such treatment remains unclear. This study evaluated the effectiveness of IVIG treatment in severe COVID-19 patients. METHODS: This retrospective multicentre study evaluated 28-day mortality in severe COVID-19 patients with or without IVIG treatment. Each patient treated with IVIG was matched with one untreated patient. Logistic regression and inverse probability weighting (IPW) were used to control confounding factors. RESULTS: The study included 850 patients (421 IVIG-treated patients and 429 non-IVIG-treated patients). After matching, 406 patients per group remained. No significant difference in 28-day mortality was observed after IPW analysis (average treatment effect (ATE) = 0.008, 95% CI -0.081 to 0.097, p 0.863). There were no significant differences between the IVIG group and non-IVIG group for acute respiratory distress syndrome, diffuse intravascular coagulation, myocardial injury, acute hepatic injury, shock, acute kidney injury, non-invasive mechanical ventilation, invasive mechanical ventilation, continuous renal replacement therapy and extracorporeal membrane oxygenation except for prone position ventilation (ATE = -0.022, 95% CI -0.041 to -0.002, p 0.028). DISCUSSION: IVIG treatment was not associated with significant changes in 28-day mortality in severe COVID-19 patients. The effectiveness of IVIG in treating patients with severe COVID-19 needs to be further investigated through future studies.


Assuntos
COVID-19/terapia , Imunoglobulinas Intravenosas/uso terapêutico , Idoso , COVID-19/diagnóstico , COVID-19/mortalidade , Feminino , Mortalidade Hospitalar , Humanos , Imunização Passiva/mortalidade , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , SARS-CoV-2 , Índice de Gravidade de Doença , Resultado do Tratamento
11.
Chemosphere ; 279: 130605, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33894512

RESUMO

Rapid and highly efficient treatment of acid mine drainage (AMD) is still challenging due to the low pH and high metal concentrations in it. This research focuses on a novel treatment method of AMD using direct contact membrane distillation (DCMD) and photocatalysis to recover water and utilize iron. In the DCMD process without pretreatment, the flux decreased by 93.38%. If pretreated by adding sodium oxalate, scale formation potential was effectively mitigated due to the removal of calcium and complexing of iron. For the treatment of the pretreated AMD (PAMD), 60% of water was recovered in the DCMD process with the flux decrease of 22%. The concentrate obtained from the DCMD process demonstrated high photocatalytic activity in the methylene blue (MB) degradation in an aqueous solution. In addition, the Fe (III)-oxalate complexes in the concentrate were reduced to insoluble Fe (II)-oxalate with visible light irradiation, which could be separated by sedimentation and used as a Fenton catalyst. Hence, this novel method exhibits great advantages on effectively inhibiting DCMD membrane fouling during AMD treatment, producing high-quality distillate with low conductivity, and realizing near zero-discharge of AMD.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Destilação , Ferro , Membranas Artificiais , Mineração , Água , Poluentes Químicos da Água/análise
12.
Rev Sci Instrum ; 92(2): 024101, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33648137

RESUMO

In this paper, we present a cable-driven exoskeleton with variable stiffness for upper limb rehabilitation. Adjustable stiffness of the cable-driven exoskeleton is achieved by attaching a novel variable stiffness module (VSM) to each driving cable. The module is able to vary stiffness in a large range through changing cable tension. In this paper, a stiffness model is developed for a cable-driven exoskeleton to reveal the stiffness performance of the exoskeleton with the influence of VSMs. Based on the stiffness model, a controller with stiffness-oriented strategy is proposed to vary the stiffness of the exoskeleton. Experiments on a prototype of a cable-driven exoskeleton are conducted to validate the controller.


Assuntos
Exoesqueleto Energizado , Reabilitação/instrumentação , Extremidade Superior , Fenômenos Biomecânicos , Desenho de Equipamento , Robótica
13.
J Agric Food Chem ; 69(14): 4319-4330, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33788563

RESUMO

Human milk (HM) is the primary source of nutrients and bioactive components that supports the growth and development of infants. However, the proteins present in human milk may change depending on the period of lactation. In this light, the objective of the present study was to evaluate the effect of lactation period on HM utilizing a data-independent acquisition (DIA) approach to identify the differences in HM whey protein proteomes. As part of the study, whey proteins of January, February, and June in human milk were studied. The results identified a total of 1563 proteins in HM whey proteins of which 114 groups were subunits of differentially expressed proteins as revealed by cluster analysis. Protein expression was observed to be affected by the period of lactation with expression levels of plasminogen, thrombospondin-1, and tenascin higher during January, keratin, type I cytoskeletal 9 highest in February, and transcobalamin-1 highest in June. The results of this study contribute to expand our understanding of the human whey proteome but also provide strong evidence for the nutritional difference of HM during different lactation periods.


Assuntos
Leite Humano , Proteômica , Animais , Aleitamento Materno , Feminino , Humanos , Lactente , Lactação , Leite , Proteínas do Leite , Proteínas do Soro do Leite
14.
Mol Med Rep ; 23(3)2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33495837

RESUMO

Prostate cancer (PCa) is a leading cause for death in men and the most commonly diagnosed malignancy globally. MicroRNA (miR)­583 expression levels have been discovered to be downregulated in recurrent PCa samples compared with non­recurrent cases. However, the precise functions and pathogenic mechanism of miR­583 in the development of PCa are vague, thus the aim of the present study was to investigate these. The expression levels of miR­583 and Janus kinase 1 (JAK1) in PCa tissues and cell lines were analyzed using reverse transcription­quantitative PCR and western blotting. The protein expression levels of phosphorylated (p)­STAT3 and STAT3 in PCa cell lines were also analyzed using western blotting. The effects of miR­583 and JAK1 on the proliferation and invasion of PCa cell lines cell lines were determined using MTT and Transwell assays, respectively. The binding interaction between miR­583 and the 3'­untranslated region of JAK1 were predicted by TargetScan, and further validated using dual luciferase reporter assays in PCa cell lines. The results revealed that the expression levels of miR­583 were downregulated, while those of JAK1 were upregulated in PCa tissues and cell lines (DU145 and PC3). The transfection with the miR­583 mimic inhibited the proliferation and invasion, as well as downregulating JAK1 and p­STAT3 protein expression levels in DU145 and PC3 cell lines. These effects were partially abolished following the overexpression of JAK1. Moreover, JAK1 was identified to be a target gene for miR­583 in DU145 and PC3 cell lines and the expression levels of miR­583 were revealed to be negatively correlated with JAK1 expression levels in PCa tissues. In conclusion, the findings of the present study suggested that miR­583 may inhibit the proliferation and invasion of PCa cells by targeting JAK1, thus providing a novel therapeutic target for patients with PCa.


Assuntos
Proliferação de Células , Janus Quinase 1/metabolismo , MicroRNAs/metabolismo , Proteínas de Neoplasias/metabolismo , Neoplasias da Próstata/metabolismo , RNA Neoplásico/metabolismo , Humanos , Janus Quinase 1/genética , Masculino , MicroRNAs/genética , Invasividade Neoplásica , Proteínas de Neoplasias/genética , Células PC-3 , Neoplasias da Próstata/genética , Neoplasias da Próstata/patologia , RNA Neoplásico/genética
15.
J Agric Food Chem ; 69(4): 1206-1213, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33481586

RESUMO

Starch biosynthesis in cereal crops is a complex pathway regulated by multiple starch synthetic enzymes. Starch synthase IIa (SSIIa) is well-known to be one of the major starch synthases and is very important in amylopectin biosynthesis. It has significant effects on grain composition and kernel traits. However, there are few reports on the association of natural variation of SSIIa in barley and grain composition and characteristics. In this work, two SSIIa isoforms were first identified as SSIIaH and SSIIaL by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, mass spectrometry, and Western blotting. Sequence analysis of the SSIIa gene demonstrated that a 33 bp insertion coding a peptide of APPSSVVPAKK caused different SSIIa, e.g., SSIIaH and SSIIaL. Based on this molecular difference, a polymerase chain reaction marker was developed, which could be used to screen different SSIIa genotypes easily. Kernel hardness of SSIIaL genotypes was significantly higher than that of SSIIaH Chinese barley cultivars. The proportion of SSIIaL genotypes was extremely low in Australian barley cultivars (5/24) and much higher in Tibetan hull-less barley cultivars (46/74), consistent with the end-use requirements of barley grain. This study provided new information in barley endosperm starch synthesis and indicated that it is valuable for choosing the preferred SSIIa genotype according to the end-use requirements.


Assuntos
Hordeum/enzimologia , Proteínas de Plantas/metabolismo , Sementes/química , Sintase do Amido/metabolismo , Sequência de Aminoácidos , Amilopectina/química , Amilopectina/metabolismo , Austrália , Hordeum/química , Hordeum/genética , Proteínas de Plantas/genética , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Sementes/enzimologia , Sementes/genética , Amido/química , Amido/metabolismo , Sintase do Amido/genética
16.
Opt Lett ; 46(2): 286-289, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33449009

RESUMO

In this Letter, the broadband operation in wavelengths from 520 nm to 980 nm is demonstrated on silicon nitride nanophotonic phased arrays. The widest beam steering angle of 65° on a silicon nitride phased array is achieved. The optical radiation efficiency of the main grating lobe in a broad wavelength range is measured and analyzed theoretically. The optical spots radiated from the phased array chip are studied at different wavelengths of lasers. The nanophotonic phased array is excited by a supercontinuum laser source for a wide range of beam steering for the first time to the best of our knowledge. It paves the way to tune the wavelength from visible to near infrared range for silicon nitride nanophotonic phased arrays.

17.
Anal Sci ; 37(7): 941-947, 2021 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32893249

RESUMO

Recently, nanozymes have become a topic of particular interest due to their high activity level, stability and biocompatibility. In this study, a visual, sensitive and selective point-of-care immunosensor was established to test the pathogen Escherichia coli O157:H7 (E. coli O157:H7). Hemin and magainin I (MI) hybrid nanocomposites (Hemin@MI) with peroxidase-mimicking activities were synthesized via a "one-pot" method, involving the simple mixing of an antimicrobial peptide (MI) against E. coli O157:H7 and hemin in a copper sulfate sodium phosphate saline buffer. Hemin@MI nanocomposites integrating target recognition and signal amplification were developed as signal probes for the point-of-care colorimetric detection of pathogenic E. coli O157:H7. Hemin@MI nanocomposites exhibit excellent peroxidase activity for the chromogenic reaction of ABTS, which allows for the visual point-of-care testing of E. coli O157:H7 in the range of 102 to 108 CFU/mL, with a limit of detection of 85 CFU/mL. These data suggest this immunosensor provides accessible and portable assessments of pathogenic E. coli O157:H7 in real samples.

18.
J Prosthet Dent ; 126(4): 471-476, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32958302

RESUMO

Precise preparation of guiding planes is essential for removable partial dentures (RPDs). This report introduces a metal template fabricated by computer-aided design and computer-aided manufacturing (CAD-CAM) to help prepare guiding planes for RPDs.


Assuntos
Prótese Parcial Removível , Desenho Assistido por Computador , Metais
19.
Biotechnol Appl Biochem ; 68(3): 560-567, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32472699

RESUMO

To prevent foodborne diseases and minimize their impacts, it is extremely important to develop a cost-effective and efficient bacterial detection assay for diagnostics, particularly in resource-poor settings. In this study, 16S rRNA from foodborne Salmonella was coupled with multiple HCR (hybridization chain reaction) concatemers and functionalized in a signal structure for lateral flow nucleic acid biosensor (LFNAB) detection. The 16S rRNA was incubated with two specific capture probes and multiple helper probes carrying the same initiator, to unwind its secondary structure and form an "initiators-on-a-string" complex. Through use of the initiators, each target 16S rRNA yielded multiple HCR concatemers tethered to numerous biotins, and numerous streptavidin-labeled gold nanoparticles were introduced on the LFNAB. The limit of detection was 53.65 CFU/mL for Salmonella. Notably, this method has high specificity and applicability for the detection of Salmonella in food and water samples.


Assuntos
Técnicas Biossensoriais , Nanoestruturas/química , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Salmonella/isolamento & purificação , Doenças Transmitidas por Alimentos/diagnóstico , Ouro/química , Humanos , Nanopartículas Metálicas/química , Salmonella/genética
20.
Expert Rev Respir Med ; 15(5): 689-696, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33336607

RESUMO

Background: Due to its high mortality rate, immediate and reliable severity assessment and accurate prediction of prognosis at hospital admission is critical for the management of community-acquired pneumonia (CAP) patients.Methods: Consecutive patients with primary diagnosis of CAP and hospitalized at our hospital from January 2013 to December 2015 were screened for this retrospective study. Demographic information, clinical and laboratory examination, severity model scoring, and 90-day outcomes were studied. Area under the curve (AUC) of receiver operating characteristic curve (ROC) was analyzed to compare the predictive value of different prognostic scoring methods.Results: 2099 CAP patients with a median age of 60 (IQR 44.0-73.0) years-old were included in this study. Median length of stay was 10 days (IQR 8.0-13.0). The all-cause 90-day mortality was found in 2.19% (46/2099) of all patients. PCT was identified as an independent predictor for the prognosis of CAP patients. CURB-65 in combination with PCT outperformed other predictive methods in 90-day mortality with the optimal AUC of 0.900 and Youden's Index of 0.706.Conclusions: PCT is a good marker for the assessment of severity and 90-day mortality of CAP patients. The combination of PCT and CURB-65 was more accurate than other prognostic models in predicting 90-day mortality.


Assuntos
Infecções Comunitárias Adquiridas , Pneumonia , Adulto , Idoso , Infecções Comunitárias Adquiridas/diagnóstico , Humanos , Pessoa de Meia-Idade , Pneumonia/diagnóstico , Pró-Calcitonina , Estudos Retrospectivos , Índice de Gravidade de Doença
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