Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 67
Filtrar
1.
BMC Musculoskelet Disord ; 24(1): 8, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36604726

RESUMO

BACKGROUND: Bipolar clavicle injury is a rare injury involving any combination of dislocation and/or fracture at both ends of the clavicle. Most reports of bipolar clavicle injury have been based on a single case, and treatment of this injury remains controversial. The present study was performed to evaluate the efficacy of surgical management with internal plating for bipolar clavicle injuries. METHODS: We performed internal plating to treat seven consecutive bipolar clavicle injuries with different injury patterns from May 2013 to June 2021. A clavicle hook plate was used for five sternoclavicular joint injuries (including a revision surgery) and three acromioclavicular joint dislocations, a T plate was used for one sternoclavicular joint injury, and an anatomic plate was used for one distal clavicle fracture. At follow-up, radiographs were assessed for bone alignment, joint congruity, fracture union or malunion, and implant failure or migration. Clinical evaluation included determination of the Disability of the Arm, Shoulder, and Hand (DASH) score; Constant-Murley score; visual analog scale (VAS) score; and complications. RESULTS: The patients were regularly followed up after the operation, and functional parameters were assessed over time. At a mean follow-up of 28.1 ± 22.0 months, each fracture had solid bone union, and each dislocation showed no sign of recurrent instability. The mean shoulder forward flexion was 159.3° ± 7.9°, and the mean DASH score was 8.8 ± 5.1. The mean Constant-Murley score was 88.9 ± 7.9, with six cases assessed as excellent and one case assessed as good. The mean VAS score was 1.0 ± 1.5, and the mean patient satisfaction score was 9.3 ± 0.8. No complications occurred, and each patient was able to resume their preinjury daily activity and was highly satisfied with their treatment. CONCLUSIONS: In the present study, internal plating for bipolar clavicle injury allowed early mobilization and resulted in good joint function. We recommend fixation of the more severely affected side first because the other side may be passively reduced and acquire stability once the more severely affected side has been fixed. Internal fixation of the other end may therefore be unnecessary unless residual instability exists.


Assuntos
Fraturas Ósseas , Luxações Articulares , Luxação do Ombro , Humanos , Clavícula/diagnóstico por imagem , Clavícula/cirurgia , Clavícula/lesões , Fixação Interna de Fraturas/efeitos adversos , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/cirurgia , Luxações Articulares/diagnóstico por imagem , Luxações Articulares/cirurgia , Luxação do Ombro/cirurgia , Placas Ósseas , Resultado do Tratamento
2.
Brain Sci ; 12(12)2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36552103

RESUMO

Sleep-wake rhythm disturbances have a negative impact on attention. However, how it affects attention and whether the restoration of regular rhythms can restore attention are unclear. This study aims to explore the effects of sleep-wake rhythm disturbances on three subfunctions of attention (alertness, orientation, and executive control) and the restoration of regular rhythms on these functions. Twenty-one participants in the experimental group (who experienced sleep-wake rhythm disturbances for at least one month; aged 18-26) were required to sleep regularly following a sleep schedule, whereas 20 participants in the control group (who maintained regular sleep for at least three months; aged 19-22) received no manipulation of their sleep. All participants were assessed using the attention network test three times in six days. All of them wore spectrum activity monitors and kept sleep diaries every day. The results showed that the effects of alertness and executive control in the experimental group were significantly lower than those in the control group. After five days of regular sleep, the difference in the alertness effect between the two groups significantly decreased. These results suggested that under natural conditions, sleep-wake rhythm disturbances could negatively influence alertness and executive control, and a short period of restoring a regular rhythm has a recovery effect on alertness.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36576686

RESUMO

Gut microbiota has been identified as a unique endocrine organ linked to the development of cardiovascular disease and other illnesses, especially deteriorated in overweight and obese postmenopausal women. The object of this systematic review and meta-analysis aimed to assess the effects of oral supplementation with probiotics for overweight and obese postmenopausal women. We performed a systematic search for randomized controlled trials (RCTs) from inception to April 2022 in MEDLINE, Embase, Cochrane Library, Web of Science, and ClinicalTrials.gov. We also performed a hand search by reviewing reference lists to identify trials. The risk of bias in individual studies was assessed with the Cochrane risk of bias tool for randomized trials (RoB). Two reviewers independently selected studies and collected data. There were 6 studies from 5 RCTs with 281 participants in this systematic review. Compared with the placebo, the probiotics supplementation group had reductions in insulin (MD - 4.20 IU/L (95% CI - 8.11 to - 0.30 IU/L), I2 = 54%), HOMA-IR (MD - 1.25 (95% CI - 2.49 to - 0.01), I2 = 50%), and TNF-α (MD - 0.12 pg/mL (95% CI - 0.22 to - 0.01 pg/mL), I2 = 44%). Improvements were also shown in body adiposity and lipid profile, but these effects were nonsignificant. In addition to body adiposity and cardiovascular risk markers, one trial showed the administration of probiotics also had an effect on iron metabolism. In conclusion, probiotics have a potential benefit on glucose metabolism and inflammatory process in overweight and obese postmenopausal women, but this effect is mild. It demonstrates that oral probiotics supplementation can be a complementary treatment for improving the fitness of postmenopausal women with overweight and obesity.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36554593

RESUMO

The global demand for sand and gravel is at 50 billion tons per year, far exceeding global resource capacities. It reached 7.6 billion tons in 2021 in the Yangtze River Basin (YRB), China. However, production is severely limited in the YRB. Therefore, the incongruity between the supply and demand of river sand is prominent. Wise management of decreasing sand resources in the YRB has become critical since the Three Gorges Dam became operational in 2003. This study synthesized spatial and temporal changes in sand mining activities and quantities along the Yangtze River and its major tributaries from 2004 to 2020. Results from the study show that the mining amount during the period reached 76.2 million tons annually. At the same time, riverine suspended sediment discharge (SSD) downstream of the Three Gorges Dam decreased largely. SSD reduction leads to riverbed erosion, further limiting the riverine sand and gravel sources for mining. Thus, alternative sand and gravel resources, as well as optimizing supply/demand balance, are necessary for sustainable development. There is an urgent need to assess the relationship between river sand resources and exploitation in the YRB for creating a sand and gravel data management system in order to cope with the increasing incongruity between their supply and demand.


Assuntos
Monitoramento Ambiental , Areia , Rios , China
5.
Ecotoxicol Environ Saf ; 248: 114278, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36379071

RESUMO

Groundwater is a very important natural resource for humanity, however, the degradation of groundwater quality caused by anthropogenic activities may pose a serious hazard to human health and ecosystem. In this study, 143 groundwater samples were analyzed to investigate the spatiotemporal variations of hydrochemistry, groundwater quality and potential human health risk of groundwater in Jinghe River Basin, Chinese Loess Plateau. Based on the mean concentrations value (MCV) and over-standard rate (OSR), the groundwater in Jinghe River Basin were divided into three groups: Group Ⅰ (high MCV: greater than standard values and high OSR: ≥ 10 %) contains TDS (total dissolved solids), TH (total harness), SO42-, F- and Fe; Group Ⅱ (low MCV and high OSR) contains pH, Cl-, NO3-, Cr6+ and As, Group Ⅲ (low MCV and low OSR) contains NO2-, NH4+, Zn, Mn, Pb, I- and Cd. The results of set pair assessment indicated that the unsuitable and doubtful class of comprehensive groundwater quality are mainly distributed in the northern part of study area, accounting for 29 % and 13.6 % of the area in 2004 and 2015, respectively. Human health risk assessment based on the triangular fuzzy number suggested that the variation of risk levels in different age groups is Children > Adult Men > Adult Women. Average carcinogenic risks of As in groundwater for the three groups exceed the acceptable level, and non-carcinogenic risk of NO3- for Children is higher than the acceptable threshold. The findings of this study provide valuable insight into the spatiotemporal characteristics of groundwater quality and potential health risks of polluted groundwater by anthropogenic activities.


Assuntos
Água Potável , Água Subterrânea , Poluentes Químicos da Água , Criança , Adulto , Feminino , Humanos , Rios , Qualidade da Água , Ecossistema , Água Potável/análise , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Medição de Risco , China
6.
Nat Chem ; 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376388

RESUMO

Iodine is a reactive trace element in atmospheric chemistry that destroys ozone and nucleates particles. Iodine emissions have tripled since 1950 and are projected to keep increasing with rising O3 surface concentrations. Although iodic acid (HIO3) is widespread and forms particles more efficiently than sulfuric acid, its gas-phase formation mechanism remains unresolved. Here, in CLOUD atmospheric simulation chamber experiments that generate iodine radicals at atmospherically relevant rates, we show that iodooxy hypoiodite, IOIO, is efficiently converted into HIO3 via reactions (R1) IOIO + O3 → IOIO4 and (R2) IOIO4 + H2O → HIO3 + HOI + (1)O2. The laboratory-derived reaction rate coefficients are corroborated by theory and shown to explain field observations of daytime HIO3 in the remote lower free troposphere. The mechanism provides a missing link between iodine sources and particle formation. Because particulate iodate is readily reduced, recycling iodine back into the gas phase, our results suggest a catalytic role of iodine in aerosol formation.

7.
Artigo em Inglês | MEDLINE | ID: mdl-36360817

RESUMO

Using typical counties in the Yimeng Mountain area of northern China as an example, this paper analyzed the household and agricultural input characteristics of different types of peasant households using survey data from 262 farm households. The target minimization of the total absolute deviations (MOTAD) model was applied to determine the optimal combinations in the allocation of agricultural input factors and production for different types of at-risk peasant households to obtain the ideal agricultural income. The relevant results are twofold. (1) The agricultural input behaviors of different types of peasant households vary significantly. The highest levels of agricultural land, labor, and yield-increasing and labor-saving inputs included I part-time peasant households (I PTPH), followed by full-time peasant households (FTPH), while the input levels of II part-time peasant households (II PTPH) and non-agricultural peasant households (NAPH) with higher levels of non-agricultural employment gradually decreased. In general, an increase in peasant households' part-time employment revealed an inverted U-shaped trend in the agricultural input level, with a trajectory of I PTPH > FTPH > II PTPH > NAPH. (2) The current agricultural inputs and production combinations of different types of peasant households have room for improvement. It is necessary to adjust agricultural inputs and optimize production combinations to obtain target incomes. Overall, all types of peasant households must streamline labor inputs and increase capital inputs, except for I PTPH, for which capital inputs should be reduced. Following optimization, economic crops gradually replace grain crops, and the optimal agricultural incomes of peasant households will be improved. The study results provide practical policy insights for reducing agricultural production risks and improving agricultural production incomes.


Assuntos
Mão de Obra em Saúde , População Rural , Humanos , Demografia , Países em Desenvolvimento , Agricultura , China , Economia
8.
Artigo em Inglês | MEDLINE | ID: mdl-36429586

RESUMO

Analyzing cultivated land input behavior (CLIB) at the scale of rural households links with cultivated land-use efficiency (CLUE), this study examined the Yimeng Mountain area in northern China, supported by field survey data from 737 rural households. This research systematically analyzed the characteristics of CLIB of different types of rural households, measured the CLUE of different types of rural households by using a data envelopment analysis (DEA) model, and explored the influence of CLIB on CLUE based on the Tobit regression model. The results show (1) significant differences in the characteristics of the CLIB of different types of rural households in the Yimeng Mountain area. Among them, the highest land, labor, and capital inputs were I part-time rural households (I PTRH), followed by full-time rural households (FTRH). In contrast, II part-time rural households (II PTRH) and non-agricultural rural households (NARH) had higher levels of non-agricultural employment; however, their input levels gradually declined. (2) The CLUE of the sample rural households was generally low and had considerable potential for improvement. Regarding the types of rural households, as the degree of part-time employment increased, the CLUE showed an inverted U-shaped trend of first increased and then decreased, namely, I PTRH > FTRH > II PTRH > NARH. This finding indicates that appropriate part-time employment could help to promote investment in agricultural production and improve the CLUE. (3) The CLIB of rural households had significant effects on CLUE; the literacy of the agricultural labor force, yield-increasing input per unit area, per capita household income, share of agricultural income, operation scale of cultivated land, effective irrigation rate of cultivated land, and soil and water conservation rate of cultivated land had positive effects on improving CLUE. Even so, there was still significant heterogeneity in the degree of influence of different rural household types. The study concluded with some policy recommendations from the perspective of different rural household types to provide references for optimizing farming inputs and improving CLUE.


Assuntos
Agricultura , População Rural , Humanos , China , Agricultura/métodos , Fazendas , Renda
9.
Artigo em Inglês | MEDLINE | ID: mdl-36361352

RESUMO

Antibiotic resistance pollution in livestock manure is a persistent issue that has drawn public attention. Vermicomposting is an ecofriendly biological process that can render livestock manure harmless and resourceful. However, little is known about the impact of vermicomposting on antibiotic resistance in livestock manure under stress caused by potentially toxic arsenic levels. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift in antibiotic resistance genes (ARGs) and related microorganisms in fresh earthworm casts as well as vermicompost product health (i.e., nutrient availability and enzyme activity) when they were fed on arsenic-contaminated cow manure. The results showed that the earthworms' interaction with cow dung led to a significant reduction in ARG concentrations, especially for tetracycline ARGs (tet-ARGs), ß-lactam ARGs (bla-ARGs), and quinolone ARGs (qnr-ARGs). However, arsenic significantly enhanced ARG accumulation in earthworm casts in a dose-dependent manner. Moreover, vermicomposting increased the percentage of Bacteroidota in the converted products. Furthermore, arsenic exposure at low concentrations promoted the proliferation of Proteobacteria, whereas high concentrations had little effect on Proteobacteria. Our study provides valuable insight into the changes in the antibiotic resistome and related microorganisms during vermicomposting of arsenic-amended cow manure, and it is crucial to explain the environmental impact of earthworms and improve our understanding of the reciprocal benefits of soil invertebrates.


Assuntos
Arsênio , Oligoquetos , Animais , Feminino , Bovinos , Esterco/microbiologia , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos/genética , Oligoquetos/genética , Solo , Gado
10.
Front Cell Infect Microbiol ; 12: 997333, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36310858

RESUMO

Background: Imbalance of oral salivary microbiota has been linked to the pathogenesis of a variety of systemic diseases, and oral bacterial species have been shown to be useful biomarkers for systemic diseases.This study aimed to characterize the alterations of oral microbiota in patients with esophageal squamous cell carcinoma (ESCC) and to evaluate the diagnostic performance of oral microbial biomarkers for ESCC. Methods: The relative abundance of flora in saliva samples was analyzed by 16S rDNA sequencing, and differences in the species present in samples from ESCC patients and healthy controls (HCs) were identified by analyzing species diversity and performing LEfSe analysis. Receiver operating characteristic (ROC) curve analysis was applied to evaluate the diagnostic performance of the characteristic bacteria individually and in combination. Results: Differences in bacterial diversity indexes were observed for the saliva of ESCC patients versus HCs (P<0.05), but principal coordinate analysis did not detect a significant difference in the composition of oral microbiota between ESCC patients and HCs (P>0.05). LEfSe analysis showed that Leptotrichia, Porphyromonas (Pg), Streptococcus, Rothia, Lactobacillus and Peptostreptococcus were more abundant in ESCC patient saliva than in HC saliva, whereas Haemophilus, Alloprevotella (All), Prevotella_7, Prevotella (Pre), Prevotella_6, Pasteurellaceae and Pasteurellales were significantly less abundant in ESCC patient saliva (P<0.05). From ROC curve analysis, Pg could detect ESCC with an area under the ROC curve (AUC) of 0.599, sensitivity of 62.2%, and specificity of 70%, whereas the ratio of Pg/Pre had an AUC of 0.791, sensitivity of 93.3%, and specificity of 62.3%. Moreover, the combination of the Pg/Pre and Pg/All ratios showed further improved diagnostic performance for ESCC (AUC=0.826) and even good sensitivity and specificity for the diagnosis of early ESCC (68.2% and 86%, respectively; AUC=0.786). Conclusion: This study shows that Pg in saliva can be used as a characteristic marker of ESCC, and the ratios of Pg/Pre and Pg/All offered significantly improved diagnostic performance, especially for early ESCC.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Humanos , Carcinoma de Células Escamosas do Esôfago/diagnóstico , Neoplasias Esofágicas/diagnóstico , Neoplasias Esofágicas/patologia , Porphyromonas gingivalis/genética , Saliva/microbiologia , Prevotella , Carcinoma de Células Escamosas/diagnóstico , Biomarcadores
11.
Chem Commun (Camb) ; 58(89): 12418-12430, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36281644

RESUMO

Photoelectrochemical (PEC) biosensing as a promising and largely developing technique has been widely applied in biological analysis in recent years because of its low background signal and high sensitivity. By utilizing suitable PEC active materials to establish a photoelectric (PE) conversion system, selective and sensitive measurements can be achieved with the help of specific biological recognition elements. PEC biosensors rely on the change of photocurrent that depends on the electron transfer process of nanomaterials. Therefore, the electron manipulation of PEC active nanomaterials is crucial for PEC sensing. In this review, from the perspective of the electron transfer manipulation of PEC active nanomaterials, we summarize the principle of PEC biosensors in three parts, i.e., generation of excited electrons in PEC active materials, introduction of specific materials for the formation of new electron transfer pathways, and separation of excited electrons in semiconductors. For each part, typical PEC biosensors are displayed and compared to reveal the superiority of different principles. In addition, current challenges of PEC biosensors are discussed, and some insight is given into the development of PEC biosensors in the future.


Assuntos
Técnicas Biossensoriais , Nanoestruturas , Técnicas Eletroquímicas/métodos , Elétrons , Técnicas Biossensoriais/métodos , Semicondutores
12.
Artigo em Inglês | MEDLINE | ID: mdl-36136183

RESUMO

Nitrate pollution of groundwater has become a global concern as it can affect drinking water quality and human health. In this paper, an extensive hydrochemical investigation was performed to assess the spatial distribution, source identification, and health risk of groundwater nitrate pollution in the Subei Lake basin. The prevalent pollutant, nitrate (NO3-), was identified based on descriptive statistical method and box plots, and most of the other parameters of groundwater samples met water standards and can be used for drinking purpose. The results showed that nearly 23.53% of groundwater samples displays the NO3- concentrations higher than the limit of 50 mg/L recommended by the World Health Organization, and the highest nitrate content (199 mg/L) is mainly distributed around the Mukai Lake. Piper triangle diagram demonstrated that the dominated anions of hydrochemical types exhibit a gradual evolving trend from HCO3- to SO42- and Cl- with increasing nitrate concentration. The correspondence analysis suggested that agricultural activities are identified as the most possible source of nitrate contamination, while the higher content of other parameters in individual groundwater samples may be controlled by natural factors. The impacts of pollutant NO3- on human health were quantified using human health risk assessment method, and results showed that the order of non-carcinogenic health risk values through drinking water intake is Infants>Children>Adult males>Adult females, and 65%, 53%, 41%, and 35% of samples exceed the acceptable risk level (hazard quotient=1), respectively. The main findings obtained from this study can provide valuable insight on drinking water safety and groundwater pollution prevention.

13.
J Am Chem Soc ; 144(38): 17330-17335, 2022 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-36075049

RESUMO

Molecular resonance can be strengthened by charge transfer, profiting chemical mechanism (CM)-related surface-enhanced Raman scattering (SERS). Herein a supramolecular assembly enabled SERS system is established by functionalizing para-sulfonatocalix[4]arene (pSC4) onto Au3Cu nanocrystals (NCs). Due to the cooperation of Au and Cu, pSC4 is directionally assembled on the surface of Au3Cu NCs via van der Waals force, enabling photoinduced and hydrogen bond-induced charge transfer, which remarkably enhances the Raman scattering of methylene blue (MB) captured by pSC4. In particular, for the C-N and C-C stretching of MB, the contributions of resonance Raman scattering increase up to 80%. In addition, the SERS system is able to display affinities of different host-guest interactions, and further employed to evaluate effects of drugs for Alzheimer's disease. In this work, charge transfer is realized by performing supramolecular assembly on the surface of plasmonic nanomaterials, providing an avenue to design CM-related and reporter-tunable SERS systems.


Assuntos
Nanopartículas , Nanoestruturas , Ouro/química , Azul de Metileno , Nanoestruturas/química , Análise Espectral Raman
14.
Front Immunol ; 13: 935374, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35911702

RESUMO

Purpose: The present study sets out to evaluate the feasibility, safety, and effectiveness of conversion surgery following induction immunochemotherapy for patients with initially unresectable locally advanced esophageal squamous cell carcinoma (ESCC) in a real-world scenario. Materials and Methods: In this multi-center, real-world study (NCT04822103), patients who had unresectable ESCC disease were enrolled across eight medical centers in China. All patients received programmed death receptor-1 (PD-1) inhibitor plus chemotherapy every 3 weeks for at least two cycles. Patients with significant relief of cancer-related clinical symptoms and radiological responsive disease were deemed surgical candidates. Feasibility and safety profile of immunochemotherapy plus conversion surgery, radiological and pathological tumor responses, as well as short-term survival outcomes were evaluated. Moreover, data of an independent ESCC cohort receiving induction chemotherapy (iC) were compared. Results: One hundred and fifty-five patients were enrolled in the final analysis. Esophagectomy was offered to 116 patients, yielding a conversion rate of 74.8%. R0 resection rate was 94%. Among the 155 patients, 107 (69.0%) patients experienced at least one treatment-related adverse event (TRAE) and 45 (29.0%) patients reported grade 3 and above TRAEs. Significant differences in responsive disease rate were observed between iC cohort and induction immunochemotherapy (iIC) cohort [objective response rate: iIC: 63.2% vs. iC: 47.7%, p = 0.004; pathological complete response: iIC: 22.4% vs. iC: 6.7%, p = 0.001). Higher anastomosis fistula rate was observed in the iC group (19.2%) compared with the iIC group (4%). Furthermore, Significantly higher event-free survival was observed in those who underwent conversion surgery. Conclusion: Our results supported that conversion surgery following immunochemotherapy is feasible and safe for patients with initially unresectable locally advanced ESCC. Both radiological and pathological response rates were significantly higher in the iIC cohort compared with those in the traditional iC cohort.


Assuntos
Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Cisplatino/uso terapêutico , Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/terapia , Carcinoma de Células Escamosas do Esôfago/terapia , Humanos , Terapia Neoadjuvante/métodos , Resultado do Tratamento
15.
Thorac Cancer ; 13(19): 2786-2791, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-35997004

RESUMO

Immune checkpoint inhibitor (ICI)-based therapies have shown promising advances for the first-line treatment of advanced or metastatic esophageal cancer (EC). However, few studies concerning the identification of patients who achieve durable response from ICIs have been previously reported. In the present study, pre- and on-treatment plasma circulating tumor DNA (ctDNA) were analyzed in 10 patients with advanced esophageal squamous cell cancer (ESCC) receiving first-line chemoimmunotherapy. Patients with decreased molecular tumor burden index (mTBI) >7% experienced longer progression-free survival (PFS) and durable clinical benefit (DCB, PFS ≥ 6 months). In addition, five patients showed stable disease at first scan, all three patients with decreased mTBI > 7% achieved DCB, while two cases with decreased mTBI ≤ 7% experienced non-DCB. Our results demonstrate that ctDNA monitor might help identify which ESCC patients respond to chemoimmunotherapy.


Assuntos
DNA Tumoral Circulante , Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Neoplasias Pulmonares , Biomarcadores Tumorais/genética , DNA Tumoral Circulante/genética , Neoplasias Esofágicas/tratamento farmacológico , Neoplasias Esofágicas/genética , Carcinoma de Células Escamosas do Esôfago/tratamento farmacológico , Humanos , Inibidores de Checkpoint Imunológico , Neoplasias Pulmonares/patologia
16.
Artigo em Inglês | MEDLINE | ID: mdl-35886568

RESUMO

There are abundant mineral resources in plateau areas, but it is difficult to extract them safely because the problem of hypoxia in plateau mines seriously affects the life and health of workers. In order to address the problem of hypoxia in the blind heading of a plateau metal mine, a three-dimensional roadway model was established based on field data of the Pulang copper mine in Yunnan province, China. The computational fluid dynamics (CFD) method was used to explore the optimal type of oxygen supply duct outlet, and to reveal the oxygen diffusion law influencing different ventilation factors. Grey correlation analysis was used to study the correlation values of the ventilation factors on the oxygen-enrichment effect in blind headings, such as forcing duct position, exhausting duct position, and extraction pressure ratio. The results demonstrated that the oxygen-enrichment effect of a slit oxygen outlet was better than that of the traditional oxygen supply method. When the direction of the oxygen outlet hole was 30° and the height above the roadway floor was 1.95 m, the oxygen increase effect was better than other forms of oxygen supply duct outlets. Grey correlation analysis revealed that the major influencing factors of the oxygen-enrichment effect in the roadway of the plateau mine, were, in descending order, as follows: forcing duct position, extraction pressure ratio, and exhausting duct position. This study has a positive guiding significance for improving the respiration environment in blind headings of plateau mines.


Assuntos
Oxigênio , Ventilação , China , Humanos , Hipóxia , Respiração
17.
Genes Dis ; 9(4): 1143-1151, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35685473

RESUMO

The aim is to explore the predictive value of salivary bacteria for the presence of esophageal squamous cell carcinoma (ESCC). Saliva samples were obtained from 178 patients with ESCC and 101 healthy controls, and allocated to screening and verification cohorts, respectively. In the screening phase, after saliva DNA was extracted, 16S rRNA V4 regions of salivary bacteria were amplified by polymerase chain reaction (PCR) with high-throughput sequencing. Highly expressed target bacteria were screened by Operational Taxonomic Units clustering, species annotation and microbial diversity assessment. In the verification phase, the expression levels of target bacteria identified in the screening phase were verified by absolute quantitative PCR (Q-PCR). Receiver operating characteristic (ROC) curves were plotted to investigate the predictive value of target salivary bacteria. LEfSe analysis revealed higher proportions of Fusobacterium, Streptococcus and Porphyromonas, and Q-PCR assay showed significantly higher numbers of Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in patients with ESCC, when compared with healthy controls (all P < 0.05). The areas under the ROC curves for Streptococcus salivarius, Fusobacterium nucleatum, Porphyromonas gingivalis and the combination of the three bacteria for predicting patients with ESCC were 69%, 56.5%, 61.8% and 76.4%, respectively. The sensitivities corresponding to cutoff value were 69.3%, 22.7%, 35.2% and 86.4%, respectively, and the matched specificity were 78.4%, 96.1%, 90.2% and 58.8%, respectively. These highly expressed Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in the saliva, alone or in combination, indicate their predictive value for ESCC.

18.
Environ Sci Pollut Res Int ; 29(50): 75936-75954, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35665453

RESUMO

The water quality of Hong Kong's four water control zones (Tolo Harbour and Channel, Port Shelter, Victoria Harbour, and Junk Bay) is of vital importance and has attracted much attention. This study aims to more objectively and comprehensively assess the water quality and its health impact based on the four-year monitoring data of 21 parameters collected from four zones. First, physicochemical characteristics of the water system were investigated based on multivariate statistical approaches, including Kruskal-Wallis test, hierarchical cluster analysis, and Mann-Kendall test. Then, water quality levels over space and time and the element sources were analyzed using adaptive-weight water quality index (AWQI) method, and factor analysis, respectively. Finally, the potential harm of trace elements for humankind was identified based on the health risk assessment model. The results revealed that (1) the values of more than half of the water quality parameters exhibited significant interannual changes, and the values of all parameters distinctly varied over space; (2) The water quality status in four water control zones showed a steady and long-term improvement trend from 2016 to 2019; (3) The sources of pollution elements impacting water quality status were related to the comprehensive influence of human activities and natural processes; (4) The carcinogenic risks of all trace elements were negligible or acceptable, while Mn and As may cause noncarcinogenic harm to humankind.


Assuntos
Oligoelementos , Poluentes Químicos da Água , Monitoramento Ambiental/métodos , Hong Kong , Humanos , Medição de Risco , Rios , Oligoelementos/análise , Poluentes Químicos da Água/análise , Qualidade da Água
19.
Artigo em Inglês | MEDLINE | ID: mdl-35627573

RESUMO

The propagation of antibiotic resistance genes (ARGs) from domestic livestock manure is an unnegligible important environmental problem. There is an increasing need to understand the role of domestic livestock manure in causing antibiotic resistance in the environment to minimize risks to human health. Here, we targeted ß-lactam resistance genes (bla genes), primarily discovered in clinical settings, to compare the high-risk ARG profile and their main spreading vectors of 26 family livestock farms in China and analyze the effects of domestic livestock manure on their receiving farmland environments. Results showed that the high-risk bla genes and their spreading carriers were widely prevalent in livestock and poultry manure from family farms. The blaampC gene encoding extended-spectrum AmpC ß-lactamases, as well as its corresponding spreading carrier (class-1 integron), had the highest occurrence level. The bla gene abundance in family chicken farms was higher than that in family swine and cattle farms, while the bla gene contamination in the feces of laying hens or beef cattle was worse than that in corresponding broiler chickens or dairy cattle. Notably, the application from domestic livestock manure led to substantial emission of bla genes, which significantly increased the abundance of high-risk resistance genes in farmland soil by 12-46 times. This study demonstrated the prevalence and severity of high-risk resistance genes in domestic livestock and poultry manure; meanwhile, the discharge of bla genes also highlighted the need to mitigate the persistence and spread of these elevated high-risk genes in agricultural systems.


Assuntos
Gado , Esterco , Animais , Bovinos , Galinhas , Fazendas , Feminino , Genes Bacterianos , Aves Domésticas , Prevalência , Suínos , Resistência beta-Lactâmica
20.
Microb Pathog ; 167: 105561, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35526679

RESUMO

African swine fever (ASF) is a severe disease affecting pigs with high economic losses and endemicity in various parts of the world. So, it represents a serious threat to the global food safety. The disease was discovered in sub-Saharan Africa where still endemic, and first case was recorded in Kenya in 1921. It is now found all over the world; in Africa, Europe, Asia, and the Pacific it already affects more than 50 countries including Republic of Korea, China, Malaysia, Germany, Bhutan, and India. The P72 protein encoded by the B646L gene is the major protein that reveals high reactogenicity and antigenicity. While the P54 plays a significant role in virus pathogenesis especially cell apoptosis. Multiple virus proteins can suppress the apoptosis of the infected cell at an early stage. The disease spreads through contact with the diseased cases, contaminated fomites, and tick bites. Meanwhile, contaminated water sources might be an essential source of infection. The recovered animals have a significant role in disease persistence as silent carriers. Multiple factors might lead to the observed disease seasonality. Route of exposure, infectious dose, and herd immunity are the main determinants of disease severity and clinical signs. The several types of PCR are well-accepted standard tests for early diagnosis. Although commercial ELISAs were stipulated by OIE, it should be combined with some other virology inspections or serological assays. The ASFV-free countries should be protected against the virus entrance especially that all developed vaccines failed to provoke enough immunity status against the challenged virus. Moreover, it accelerates the speed of revealing clinical symptoms.


Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , África , Febre Suína Africana/epidemiologia , Febre Suína Africana/prevenção & controle , Vírus da Febre Suína Africana/genética , Animais , Europa (Continente) , Suínos , Proteínas Virais/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...