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1.
J Mol Diagn ; 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32470556

RESUMO

Tuberculosis is one of the most serious infectious diseases resulting in lethal death worldwide. Traditional detection methods are still not enough to meet the clinical requirements of rapid diagnosis, high specificity and sensitivity. Fast, sensitive and accurate detection of mycobacterium tuberculosis (MTB) is an urgent need for the treatment and control of tuberculosis disease. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated proteins (Cas12a) exhibits strong nonspecific degradation ability of exogenous single-strand nucleic acids (trans-cleavage) after specific recognition of target sequence. We purified Cas12a protein and selected a proper guide RNA (gRNA) based on conserved sequences of MTB from designed gRNA library. Then, we proposed a novel detection method based on recombinase polymerase amplification (RPA) and CRISPR/Cas12a nuclease system for specific and sensitive detection of MTB DNA. The assay based on fluorescence detection showed 4.48 fM of limit of detection (LOD) and good linear correlation of concentration with fluorescence value (R2=0.9775). Also, it showed good performance in distinguishing other bacteria. Furthermore, its clinical performance was evaluated by 193 samples and showed sensitivity of 99.29% (139/140) and specificity of 100% (53/53) at 99% confidence interval, respectively, compared with culture method. Taken together, the CRISPR/Cas12a system showed good specificity, excellent sensitivity and accuracy for MTB detection, and it meets requirements of MTB detection in clinical samples and has great potential for clinical translation.

2.
Environ Sci Technol ; 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32453946

RESUMO

1,8-diazabicyclo[5.4.0]-undec-7-ene (DBU)-glycerol solution is employed as a promising CO2 absorbent. The regeneration of the CO2-loaded solution is of vital importance for its commercialization. It was investigated and compared with 30 wt.% monoethanolamine (MEA). Variables affecting the absorption and desorption processes were studied, including the solvent composition, reaction temperature and agitation. It shows that the absorption capacity for DBU-glycerol is comparable with 30 wt.% MEA, and the desorption ratio for CO2-loaded DBU-glycerol mixture can reach as high as 95 % in 60 min, 363 K at the 3:1 molar ratio of DBU to glycerol, while it is only 68 % even after 165 min for CO2-saturated 30 wt.% MEA. DBU-glycerol solution has higher cycling CO2 loading than 30 wt.% MEA. Its cyclic capacity could keep above 90 % after 10 cycles of absorption-desorption experiments. The desorption reaction is irreversible at the initial stage and the reaction rate is expected as a first-order reaction from 349 K to 377 K, and the apparent activation energy is 68.94 kJ/mol. Moreover, the heat duty of the reboiler during regeneration is estimated to be reduced by about 27 % when compared with 30 wt.% MEA.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32456116

RESUMO

Recently, with the rapid increase of urban population and industrial agglomeration, the price of construction land has increased, and construction land has become increasingly scarce. Therefore, how to improve the construction land use quality (CLUQ) becomes more and more important. The purpose of the study is to evaluate CLUQ in China's major cities and to analyze the dominant obstacle factors for quality improvement in order to provide policy advice for construction land management. This study adapts the data from 2014 to 2016 and constructs the evaluation framework of CLUQ involving economic quality, social quality, and ecological quality of construction land to evaluate and analyze CLUQ with the synthetic evaluation model, coupling evaluation model, and obstacle diagnosis model (ECO model). This study shows that the synthetic CLUQ of 23 cities out of 36 major cities in China shows a general increasing state. The economic quality of 26 cities out of 36 major cities in China has increased, while the social and ecological quality of 20 out of 36 major cities in China has decreased. In terms of spatial characteristics, the synthetic quality in the east and southwest of China is relatively high; in terms of spatial trend, the synthetic quality in longitude increases from west to east, and it shows an inverted U-shaped state in latitude. Moreover, economic development is the main obstacle factor for the improvement of CLUQ in Hohhot, Lanzhou, Urumqi, and Changchun. Social development results in the CLUQ lagging in Beijing, Guiyang, Shanghai, Xining, and Chongqing. Ecological development has a negative impact in that of Harbin, Qingdao, and Wuhan. Furthermore. The improvement of CLUQ lies in the coupling and coordinated development of economic, social, and ecological quality. For those with a low coupling degree, the targeted suggestions are given for different types based on city's quadrant distribution.

4.
Biochem Cell Biol ; 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32413267

RESUMO

It has been found that some circular RNAs (circRNA) are abnormally expressed in the process of myocardial fibrosis, but the mechanism of them is still unknown. In the process of inducing cardiac fibroblast (CF) activation with TGF-ß1 or Ang II, circRNA circ_BMP2K and miR-455-3p expression was significantly inhibited, and SUMO1 expression was promoted. Dual luciferase reporter gene assay, RIP and pull down assay showed that miR-455-3p bound with circ_BMP2K directly, and they promoted each other's expression. SUMO1 was a target gene of miR-455-3p, and circ_BMP2K enhanced the inhibition of miR-455-3p on SUMO1 expression. Further studies showed that both circ_BMP2K and miR-455-3p inhibited alpha-SMA, type I and type III collagen expression, while SUMO1 promoted their expression, and miR-455-3p inhibitors or SUMO1 overexpression reversed these regulatory effects of circ_BMP2K and miR-455-3p. Circ_BMP2K and miR-455-3p inhibited the proliferation and migration of CFs and promoted their apoptosis, but SUMO1 had the opposite effects; miR-455-3p inhibitors or SUMO1 overexpression reversed the regulation of circ_BMP2K and miR-455-3p on biological behaviors. Therefore, circ_BMP2K promotes miR-455-3p expression and then down-regulates SUMO1 expression, and finally inhibits the activation, growth and migration of CFs. These findings may provide important targets and theoretical basis for regulating the process of myocardial fibrosis.

5.
Environ Sci Technol ; 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32412239

RESUMO

The chlorinated nitroaromatic antibiotic chloramphenicol (CAP) is a refractory contaminant that is widely present in various environments. However, few CAP-mineralizing bacteria have been documented, and a complete CAP catabolism pathway has yet to be identified. In this study, the bacterial strain Sphingobium sp. CAP-1 was isolated from an activated sludge sample and was shown to be capable of aerobically subsisting on CAP as the sole carbon, nitrogen and energy source while simultaneously and efficiently degrading CAP. p-Nitrobenzoic acid (PNBA), p-nitrobenzaldehyde (PNBD), protocatechuate (PCA), and the novel side chain C3-hydroxy oxygenated product of CAP (O-CAP) were identified during CAP degradation. Strain CAP-1 was able to convert O-CAP to intermediate product PNBA. The putative functional genes associated with PNBA catabolism into the tricarboxylic acid cycle via PCA and floc formation were also identified by genome sequencing and comparative proteome analysis. A complete pathway for CAP catabolism was proposed. The discovery of a novel CAP oxidation/detoxification process and a complete pathway for CAP catabolism enriches the fundamental understanding of the bacterial catabolism of antibiotics, providing new insights into the microbial-mediated fate, transformation and resistance risk of CAP in the environment. The molecular basis of CAP catabolism and floc formation in strain CAP-1 also offers theoretical guidance for the enhanced bioremediation of CAP-containing environments.

6.
Chem Commun (Camb) ; 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32451518

RESUMO

The low-coordinated sites of electrocatalysts favour hydrogen evolution, while the edge sites are active for CO2 reduction. Oleylamine is used to stabilize nanoparticles by adsorbing on the low-coordinated sites. The hydrogen evolution reaction was dramatically suppressed and the FECO remained >93% from -0.4 to -0.8 V (vs. RHE) when oleylamine ligands existed on the surface of a gold catalyst. More H+ and electrons were involved in the CO evolution reaction, which changed the rate-limiting step from single-electron transfer to the chemical reaction step. The results establish that the surface-adsorbed surfactants during catalyst synthesis have an important effect on CO2 electrocatalytic reduction.

7.
Disabil Rehabil ; : 1-12, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32419508

RESUMO

Purpose: To determine whether shock wave therapy could improve symptoms and hand function in patients with carpal tunnel syndrome.Materials and methods: Medline, Embase, Physiotherapy Evidence Database, the Cochrane Central Register of Controlled Trials, OpenGrey, CNKI, VIP, Wang Fang databases, and China Biological Medicine were searched, from inception to December 2019.Results: Ten trials involving 433 patients (501 wrists) were included in this systematic review. The shock wave therapy was observed to have a significant effect on pain relief (MD: -0.60, 95% CI: -1.16 to -0.05, p = 0.03), syndrome alleviation (MD: -2.26, 95% CI: -3.24 to -1.27, p < 0.00001) and functional recovery (MD: -1.25 95% CI: -2.08 to -0.43, p = 0.003) among the carpal tunnel syndrome patients. As revealed by the subgroup analysis, radial shock wave therapy made a significant difference in pain relief, syndrome alleviation, and functional recovery (p < 0.05). Focused shock wave had no significant effect on pain relief, syndrome alleviation, and functional recovery (p > 0.05).Conclusion: Shock wave therapy could be conductive to improve syndrome and hand function for carpal tunnel syndrome patients.Implications for rehabilitationShock wave therapy is beneficial for alleviating syndrome and improving hand function of carpal tunnel syndrome patients.Radial shock wave therapy seems superior to focused shock wave therapy on syndrome alleviation and functional recovery of hand in carpal tunnel syndrome patients.

8.
J Med Internet Res ; 22(4): e17234, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32347802

RESUMO

BACKGROUND: Cancer has become the second leading cause of death globally. Most cancer cases are due to genetic mutations, which affect metabolism and result in facial changes. OBJECTIVE: In this study, we aimed to identify the facial features of patients with cancer using the deep learning technique. METHODS: Images of faces of patients with cancer were collected to build the cancer face image data set. A face image data set of people without cancer was built by randomly selecting images from the publicly available MegaAge data set according to the sex and age distribution of the cancer face image data set. Each face image was preprocessed to obtain an upright centered face chip, following which the background was filtered out to exclude the effects of nonrelative factors. A residual neural network was constructed to classify cancer and noncancer cases. Transfer learning, minibatches, few epochs, L2 regulation, and random dropout training strategies were used to prevent overfitting. Moreover, guided gradient-weighted class activation mapping was used to reveal the relevant features. RESULTS: A total of 8124 face images of patients with cancer (men: n=3851, 47.4%; women: n=4273, 52.6%) were collected from January 2018 to January 2019. The ages of the patients ranged from 1 year to 70 years (median age 52 years). The average faces of both male and female patients with cancer displayed more obvious facial adiposity than the average faces of people without cancer, which was supported by a landmark comparison. When testing the data set, the training process was terminated after 5 epochs. The area under the receiver operating characteristic curve was 0.94, and the accuracy rate was 0.82. The main relative feature of cancer cases was facial skin, while the relative features of noncancer cases were extracted from the complementary face region. CONCLUSIONS: In this study, we built a face data set of patients with cancer and constructed a deep learning model to classify the faces of people with and those without cancer. We found that facial skin and adiposity were closely related to the presence of cancer.

9.
Microb Cell Fact ; 19(1): 80, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32228591

RESUMO

BACKGROUND: The bioconversion of phytosterols into high value-added steroidal intermediates, including the 9α-hydroxy-4-androstene-3,17-dione (9-OHAD) and 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), is the cornerstone in steroid pharmaceutical industry. However, the low transportation efficiency of hydrophobic substrates into mycobacterial cells severely limits the transformation. In this study, a robust and stable modification of the cell wall in M. neoaurum strain strikingly enhanced the cell permeability for the high production of steroids. RESULTS: The deletion of the nonessential kasB, encoding a ß-ketoacyl-acyl carrier protein synthase, led to a disturbed proportion of mycolic acids (MAs), which is one of the most important components in the cell wall of Mycobacterium neoaurum ATCC 25795. The determination of cell permeability displayed about two times improvement in the kasB-deficient strain than that of the wild type M. neoaurum. Thus, the deficiency of kasB in the 9-OHAD-producing strain resulted in a significant increase of 137.7% in the yield of 9α-hydroxy-4-androstene-3,17-dione (9-OHAD). Ultimately, the 9-OHAD productivity in an industrial used resting cell system was reached 0.1135 g/L/h (10.9 g/L 9-OHAD from 20 g/L phytosterol) and the conversion time was shortened by 33%. In addition, a similar self-enhancement effect (34.5%) was realized in the 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) producing strain. CONCLUSIONS: The modification of kasB resulted in a meaningful change in the cell wall mycolic acids. Deletion of the kasB gene remarkably improved the cell permeability, leading to a self-enhancement of the steroidal intermediate conversion. The results showed a high efficiency and feasibility of this construction strategy.

10.
Pathol Res Pract ; : 152943, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32279917

RESUMO

BACKGROUND: Endometrial carcinoma (EC) is a genetic disease, normally accompanied by gene mutations or abnormal expression patterns. However, PTEN mutation and its prognostic value in EC remained debated. Meanwhile, the distribution of PTEN mutation, as well as its correlation with clinical characteristics and tumor immune infiltrating cells, is still poorly understood. METHODS: We conducted a comprehensive analysis of PTEN mutation based on The Cancer Genome Atlas (TCGA) database, including 525 uterine corpus endometrial carcinoma (UCEC) samples. We analyzed the frequency of PTEN mutation, distribution of PTEN mutation in different clinical characteristics, the prognostic value of PTEN mutation, and the correlation with tumor immune infiltrating cells in tumor microenvironment. RESULTS: PTEN mutation was detected in 65.5﹪of total EC samples. PTEN mutation was significantly associated with age, histological type, clinical stage, and grade. In addition, the patients with PTEN mutation showed a significantly prolonged overall survival (OS) time and disease free survival (DFS) time compared with EC patients without PTEN mutation in entire group, training group, and validation group. Multivariate Cox regression analyses suggested that PTEN mutation was an independent prognostic factor in DFS. Moreover, the percentages of Tregs (P = 0.014) and M1 macrophages (P = 0.013) were significantly different in PTEN mutation group and non-mutation group. CONCLUSION: PTEN mutation was correlated with favorable prognosis in EC patients. In addition, PTEN mutation was found to be associated with immune infiltrating cells in tumor microenvironment. Taken together, these findings suggested that PTEN could be regarded a potential predictive and therapeutic target for EC.

11.
Phys Med Biol ; 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32320959

RESUMO

PURPOSE: A convolutional neural network based tumor localization method with single x-ray projection was previously developed by us. One finding is that the intensity discrepancy between digitally reconstructed radiograph (DRR) of 3D-CT and measured x-ray projection has an impact on the performance. To address the issue, a patient-dependent intensity matching process for 3D-CT was performed using 3D-CBCT from the same patient, which was sometimes inefficient and may adversely affect the clinical implementation of the framework. To circumvent this, in this work, we propose and validate a patient-independent intensity matching method based on conditional Generative Adversarial Networks (cGAN). METHODS: A 3D cGAN was trained to approximate the mapping from 3D-CT to 3D-CBCT from previous patient data. By applying the trained network to new patient, synthetic 3D-CBCT could be generated without the need to perform actual CBCT scan on that patient. The DRR of synthetic 3D-CBCT was subsequently utilized in our CNN-based tumor localization scheme. The method was tested on 12 patient data with same imaging parameters. The resulting 3D-CBCT and DRR were compared with real ones to demonstrate the efficacy of the proposed method. The tumor localization errors were also analyzed. RESULTS: The difference between the synthetic and real 3D-CBCT had median value no more than 10 HU for all patients. The relative error between DRR and measured x-ray projection was less than 4.8%±2.0% for all patients. For the three patients with visible tumor in x-ray projections, the average tumor localization errors were below 1.7 mm and 0.9 mm in the superior-inferior (SI) and lateral directions. CONCLUSION: A patient-independent CT intensity matching method was developed, based on which accurate tumor localization can be achieved. It does not require actual CBCT scan to be performed before treatment for each patient, therefore making it more efficient in the clinical workflow.

12.
Sci Rep ; 10(1): 6699, 2020 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-32300123

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

13.
Medicine (Baltimore) ; 99(9): e19380, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32118785

RESUMO

INTRODUCTION: The "logsplitter injury" is a special type of ankle fractures that results from high energy violence with combined rotational forces and axial loads. So far, the diagnose and treatment of "logsplitter injury" remain largely unsettled and related literature is rare. PATIENT CONCERNS: An 18-year-old male fell from a fence and got his left ankle injured with severe malformation and swollen condition. No open wound was observed. DIAGNOSIS: Logsplitter injury, ankle fracture (AO/OTA classification 44C1.1, Lauge-Hansen classification: pronation-external rotation). INTERVENTIONS AND OUTCOMES: The patient was initially treated by internal fixation of fibular, repair of deltoid ligaments, and 1 syndesmotic screw fixation. When the X-ray applied after surgery, another 2 syndesmotic screws were performed to enhance stability. The syndesmotic screws were removed at 12-week and 16-week respectively. The patient was allowed for full weight-bearing immediately. However, the syndesmotic space was slightly increased compared to the contralateral side in CT views at 1-year follow-up, the function outcome was satisfied. CONCLUSION: The logsplitter injury is a high-energy ankle fracture that requires both axial and rotational load. It is categorized as 44B or 44C by the AO/OTA classification. In the classification scheme of Lauge-Hansen, our case is in line with the pronation-external rotation classification. Anatomic reduction and fixation of ankle syndesmotic injuries are required to restore the biomechanics of the ankle joint so that long-term complications can be prevented. How to fixation the syndesmosis, whether to reconstruct the deltoid ligament remains in debate in the treatment of logsplitter injury, whether and when to remove the syndesmotic screws were still debated. Correct surgical intervention is successful in the treatment of "logsplitter injury", however, the optimal fixation of syndesmosis and repair of deltoid ligaments need further investigate.


Assuntos
Fraturas do Tornozelo/cirurgia , Procedimentos Ortopédicos/normas , Acidentes por Quedas , Adolescente , Humanos , Fixadores Internos , Masculino , Procedimentos Ortopédicos/métodos , Procedimentos Ortopédicos/estatística & dados numéricos , Radiografia/métodos
14.
Chemosphere ; 252: 126478, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32197179

RESUMO

Biological denitrification is an environmentally sound pathway for the elimination of nitrogen pollution in wastewater treatment. Extreme environmental conditions, such as the co-existence of toxic organic pollutants, can affect biological denitrification. However, the potential underlying mechanism remains largely unexplored. Herein, the effect of a model pollutant, hydroxyethane-(1,1-bisphosphonic acid) (HEDP), a widely applied and consumed bisphosphonate, on microbial denitrification was investigated by exploring the metabolic and transcriptional responses of an isolated denitrifier, Pannonibacter sp. strain DN. Results showed that nitrate removal efficiency decreased from 85% to 50% with an increase in HEDP concentration from 0 to 3.5 mM, leading to nitrite accumulation of 204 mg L-1 in 3.5 mM HEDP. This result was due to the lower bacterial population count and reduction in the live cell percentage. Further investigation revealed that HEDP caused a decrease in membrane potential from 0.080 ±â€¯0.005 to 0.020 ±â€¯0.002 with the increase in HEDP from 0 to 3.5 mM. This hindered electron transfer, which is required for nitrate transformation into nitrogen gas. Moreover, transcriptional profiling indicated that HEDP enhanced the genes involved in ROS (O2-) scavenging, thus protecting cells against oxidative stress damage. However, the suppression of genes responsible for the production of NADH/FADH2 in tricarboxylic acid cycle (TCA), NADH catalyzation (NADH dehydrogenase) in (electron transport chain) ETC system and denitrifying genes, especially nor and nir, in response to 2.5 mM HEDP were identified as the key factor inhibiting transfer of electron from TCA cycle to denitrifying enzymes through ETC system.

15.
J Infect Dis ; 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32115640

RESUMO

BACKGROUND: Encephalitis in Hand, Foot and Mouth Disease is a serious threat to children's health and life. Toll-like receptor 3 is an innate immune-recognition receptor which can recognize virus and initiate innate immune responses. Emodin have the effects of anti-inflammatory and regulating immune function, but the mechanism is not very clear. METHODS: Cells and mice were pre-treated with CVB3 and treated with emodin. The mRNA and protein levels of TLR3 and downstream molecules were respectively detected by qT-PCR and Western-Blotting. TLR3 expression was also down-regulated by anti-TLR3Ab or siRNA. Pathological changes were assessed with H&E staining. Immunohistochemistry was used to examine the expression of TLR3 in brain tissues.The expression of IL-6, NF-κB and IFN-ß in serum were tested with ELISA. RESULTS: Emodin decreased the mRNA and protein levels of TLR3 and downstream molecules in vitro and in vivo. After down-regulating TLR3 using anti-TLR3Ab or siRNA, emodin could still decrease the mRNA and protein levels of TLR3 and downstream molecules. Emodin also displayed notable effects on pathology, TLR3 protein in brain tissues and expression of IL-6, NF-κB, IFN-ß in serum. CONCLUSIONS: Emodin exerts a protective effect in CVB3 mediated-encephalitis in HFMD by inhibiting the TLR3 pathway.

16.
J Phys Chem Lett ; 11(6): 2230-2240, 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32134665

RESUMO

An approach is reported for the exclusive production of CdTe magic-size clusters (MSCs) that exhibit an optical absorption doublet peaking at 385/427 nm, with an explanation of the synthesis procedure. The MSCs, defined as dMSC-427, were produced from the reaction of cadmium oleate (Cd(OA)2) and tri-n-octylphosphine telluride in octadecene at 100 °C, with the addition of acetic acid (HOAc) or acetate (M(OAc)2) during the prenucleation stage (40 °C). Without such an addition or when it was performed in the postnucleation stage (100 °C), quantum dots (QDs) developed. The production of dMSC-427 or QDs is hypothesized to be related to the solubility of the Cd precursor, such as Cd(OA)1(OAc)1 or Cd(OA)2, respectively. Also, the reactions that lead to Cd(OA)1(OAc)1 are proposed. The present study provides an in-depth understanding of the two-pathway model proposed for the prenucleation stage of binary colloidal QDs, as well as of the formation of MSCs and/or QDs.

17.
Adv Mater ; : e1907995, 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32187764

RESUMO

Metal-organic frameworks (MOFs) are promising materials for onboard hydrogen storage thanks to the tunable pore size, pore volume, and pore geometry. In consideration of pore structures, the correlation between the pore volume and hydrogen storage capacity is examined and two empirical equations are rationalized to predict the hydrogen storage capacity of MOFs with different pore geometries. The total hydrogen adsorption under 100 bar and 77 K is predicted as ntot = 0.085× Vp - 0.013× Vp 2 for cage-type MOFs and ntot = 0.076× Vp - 0.011× Vp 2 for channel-type MOFs, where Vp is the pore volume of corresponding MOFs. The predictions by these empirical equations are validated by several MOFs with an average deviation of 5.4%. Compared with a previous equation for activated carbon materials, the empirical equations demonstrate superior accuracy especially for MOFs with high surface area (i.e., SBET over ≈3000 m2 g-1 ). Guided by these empirical equations, a highly porous Zr-MOF NPF-200 (NPF: Nebraska Porous Framework) is examined to possess outstanding hydrogen total adsorption capacity (65.7 mmol g-1 ) at 77 K and record high volumetric working capacity of 37.2 g L-1 between 100 and 5 bar at 77 K.

18.
Biosci Rep ; 40(2)2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32095823

RESUMO

Tumor immune infiltration cells (TIICs) are highly heterogeneous, not only in different cancer subtypes but also within different cancer regions. We conducted the Cell-type Identification using Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) method. We assessed the relative proportions of 22 TIICs in HNSC using publicly available TCGA transcriptional datasets, analyzed the proportions of TIICs between HNSC tissues and normal tissues, along with accompanying clinicopathological data, and the impact of TIICs on clinical outcome. After the filter criteria, a total of 395 patients were included in the analysis. We found significant differences in naïve B cells, monocytes, resting mast cells, activated mast cells, CD8+ T cells, and M0 macrophages between HNSC tissues and adjacent non-cancer tissues. We also found that some TIIC subgroups were significantly associated with clinical parameters. Moreover, the patients with low Tregs fraction had worse OS and DFS than those with high Tregs fraction. However, low M0 macrophages fraction was associated with better OS and DFS in HNSC patients. Moreover, Tregs and M0 macrophages are likely to be important determinants of prognosis, which may serve as a potential immunotherapy target for HNSC. Then, we screened the immune-related differentially expressed genes (DEGs), performed the GO and KEGG enrichment analysis, constructed the protein-protein interaction network, and screened the prognosis-related hub genes in HNSC. However, further clinical investigation and basic experiments are needed to validate our results, and uncover the molecular mechanisms interlinking TIICs in HNSC and their roles in prognosis and therapy.

19.
J Cell Mol Med ; 24(6): 3560-3571, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32039564

RESUMO

Numerous studies have demonstrated that thioredoxin-interacting protein (TXNIP) expression of peripheral blood leucocytes is increased in coronary artery disease (CAD). However, the molecular mechanism of this phenomenon remained unclear. DNA methylation plays important roles in the regulation of gene expression. Therefore, we speculated there might be a close association between the expression of TXNIP and methylation. In this study, we found that compared with controls, DNA methylation at cg19693031 was decreased in CAD, while mRNA expressions of TXNIP and inflammatory factors, NLRP3, IL-1ß, IL-18, were increased. Methylation at cg19693031 was negatively associated with TXNIP expression in the cohort, THP-1 and macrophages/foam cells. Furthermore, Transwell assay and co-cultured adhesion assay were performed to investigate functions of TXNIP on the migration of THP-1 or the adhesion of THP-1 on the surface of endothelial cells, respectively. Notably, overexpressed TXNIP promoted the migration and adhesion of THP-1 cells and expressions of NLRP3, IL-18 and IL-1ß. Oppositely, knock-down TXNIP inhibited the migration and adhesion of THP-1 and expressions of NLRP3, IL-18. In conclusion, increased TXNIP expression, related to cg19693031 demethylation orientates monocytes towards an inflammatory status through the NLRP3 inflammasome pathway involved in the development of CAD.

20.
J Hazard Mater ; 392: 122321, 2020 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-32092653

RESUMO

The biological treatment of antibiotic-containing wastewater is a mainstream process, but the antibacterial activity from the persistence of antibiotics would inhibit the biological activity and function of wastewater treatment plants and lead to the risk of transmission of antibiotic resistant bacteria and genes. In this study, UV photolysis was selected as an appropriate pretreatment technology for antibiotic-containing wastewater. It could decompose many kinds of antibiotics and was not inhibited by the coexisting organics in wastewater. The antibacterial activities of five kinds of antibiotics, which were eliminated with UV irradiation, exhibited a significantly positive correlation with their parent compound concentrations. The photodecomposition of the main functional groups in antibiotics contributed to the elimination of antibacterial activity. Defluorination was the main pathway to eliminate the antibacterial activity of antibiotics containing a fluorine substituent (e.g., florfenicol and ofloxacin), while the photoinduced opening of the ß-lactam ring was the most efficient route to eliminate the antibacterial activity of ß-lactam antibiotics (e.g. cefalexin, amoxicillin and ampicillin). These results demonstrated that UV photolysis could be adopted as an efficient and promising pretreatment strategy for the source control of antibiotic antibacterial activity by the decomposition of antibiotic functional groups before the biological treatment unit.

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