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1.
Artigo em Inglês | MEDLINE | ID: mdl-32030861

RESUMO

Low-dimensional Sn-based perovskites exhibit excellent oxidation and moisture resistance, which play an irreplaceable role in lead-free perovskite photovoltaic devices. Herein, 1,4-butanediamine (BEA) is incorporated into FASnI 3 (FA = formamidinium), developing a new series of low-dimensional Dion-Jacobson (LDDJ) phase perovskite of (BEA)FA n-1 Sn n I 3n+1 , which promote the expansion of new low-dimensional lead-free perovskite. The broadness of representative (BEA)FA 2 Sn 3 I 10 bandgap appears to be softly influenced by the structural distortion due to high symmetry. The introduction of BEA ligand stabilizes the low-dimensional perovskite structure (formation energy ≈ 10 6 j/mol), which inhibits the oxidation of Sn 2+ . In addition, the compact (BEA)FA 2 Sn 3 I 10 dominated film enables a weakened carrier localization mechanism with charge transfer time is only 0.36 ps among the quantum wells, which resulting in carrier diffusion length over 450 nm for electrons and 340 nm for holes, respectively. Solar cell fabrication with (BEA)FA 2 Sn 3 I 10 delivers a champion power conversion efficiency (PCE) of 6.43% with negligible hysteresis. Particularly, the devices can retain over 90% (< 60% for FASnI 3 devices) of their initial PCEs after 1000 h without encapsulation under N 2 environment and attenuate by 12% (vanish for FASnI 3 devices within 72 h) of their initial PCEs under ambient atmospheric condition for 200 h.

2.
Am J Mens Health ; 14(1): 1557988320903200, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32005088

RESUMO

Definitive diagnosis and selection of effective treatment for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) are frustrations encountered frequently by urology care providers in their practice. Knowledge of etiology and pathophysiology is not sufficient and therapeutic guidelines have not yielded acceptable outcomes and prognoses for both patients and care providers. The authors present updated perspectives on CP/CPPS, including definition, diagnosis, treatment, and prognosis, based on literature review and clinical experience. A key point is to shift the diagnostic and therapeutic focus from a single entity of disease toward associated symptoms of CP/CPPS. An individualized multimodal treatment approach to cope with the course of the disorder is proposed. Communications and personal/family/community supports are emphasized as an important component in the therapeutic regime and rehabilitation of patients with CP/CPPS. The purpose is to improve comprehension on CP/CPPS and to help care providers and patients to achieve the goal of medical intervention-relieving associated symptoms of CP/CPPS and improving the quality of life.

3.
J Mol Neurosci ; 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32036579

RESUMO

Focal cortical dysplasia (FCD) is the main cause of medically intractable pediatric epilepsy. Previous studies have suggested that alteration of cortical interneurons and abnormal cytoarchitecture have been linked to initiation and development for seizure. However, whether each individual subpopulation of cortical interneurons is linked to distinct FCD subtypes remains largely unknown. Here, we retrospectively analyzed both control samples and epileptic specimens pathologically diagnosed with FCD types Ia, IIa, or IIb. We quantified three major interneuron (IN) subpopulations, including parvalbumin (PV)-, somatostatin (Sst)-, and vasoactive intestinal peptide (Vip)-positive INs across all the subgroups. Additionally, we calculated the ratio of the subpopulations of INs to the major INs (mINs) by defining the total number of the PV-, Sst-, and Vip-INs as mINs. Compared with the control, the density of the PV-INs in FCD type IIb was significantly lower, and the ratio of PV/mINs was lower in the superficial part of the cortex of the FCD type Ia and IIb groups. Interestingly, we found a significant increase in the ratio of Vip/mINs only in FCD type IIb. Overall, these results suggest that in addition to a reduction in PV-INs, the increase in Vip/mINs may be related to the initiation of epilepsy in FCD type IIb. Furthermore, the increase in Vip/mINs in FCD type IIb may, from the IN development perspective, indicate that FCD type IIb forms during earlier stages of pregnancy than FCD type Ia.

4.
Pregnancy Hypertens ; 19: 170-176, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-32018223

RESUMO

BACKGROUND: A major cause of preeclampsia is the placental ischemia caused by insufficient trophoblast cells, invading into the spiral artery. Storkhead-box protein 1 (STOX1) is highly associated with preeclampsia. Meanwhile, low-dose aspirin for patients with preeclampsia is effective in reducing the incidence of preeclampsia. The aim of the present study was to explore the underlying mechanism, and the relationship between STOX1 and aspirin in preeclampsia. METHODS: The human choriocarcinoma cell line JEG-3 was employed to mimic trophoblast cells and establish a model for trophoblast cells overexpressing STOX1 and knockdown of JEG cell lines, which were treated with aspirin afterwards. Cell counting kit-8 (CCK-8) assay was utilized to estimate cell proliferation and optimal concentration of aspirin for further experiments. Meanwhile, transwell assay was used to detect migration, and flow cytometry was used to measure apoptosis. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting were applied to analyze the expression levels of STOX1 and related genes. RESULTS: Overexpression of STOX1 inhibited proliferation of JEG-3 cells through epidermal growth factor (EGF), vascular EGF (VEGF), and transforming growth factor beta 1 (TGF-ß1) proteins, while suppressed migration through MMP2, MMP9, and E-cadherin proteins. In contrast, apoptosis of JEG-3 cells was elevated by STOX1 through Bcl-2, Bax, and Cox-2 proteins. Furthermore, we found that aspirin modulated the expression level of STOX1 and reversed proliferation and migration of STOX1-induced insufficient trophoblast cells. CONCLUSION: The present study suggested that inhibition of the expression of STOX1 could promote the effects of aspirin in the treatment of preeclampsia.

6.
Chin J Traumatol ; 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-32081450

RESUMO

PURPOSE: The purpose of this study was to review the microsurgical anatomy and clipping of ruptured anterior communicating artery (AComA) aneurysms and to plan and avoid complications before operation. METHODS: A total of 523 cases of cerebral aneurysms admitted to the neurosurgery department of the Third Affiliated Hospital of Sun Yat-Sen University from September 2010 to October 2018 were analyzed retrospectively. Among them, 85 patients had ruptured AComA aneurysms. This study was limited to 85 of these cases, whose satisfactory preoperative angiographic diagnostic films can be retrieved from the hospital database system because of the need for detailed review. RESULTS: We performed supraorbital eyebrow keyhole approach (SOEK) craniotomy in 85 patients to clip 85 AComA aneurysms, in the setting of subarachnoid hemorrhage (SAH). Patients' mean age was 52.69 ± 9.94 years (range, 28-78 years). The proportions of small, medium and large aneurysms were 83.5%, 15.3%, and 1.2%, respectively. The average size of the aneurysms was 5.07 ± 2.36 mm. There were 77.8% of patients with inferior aneurysms and 81.3% of patients with superior aneurysms achieved good results. There was a significant correlation between A1 dominance and operation method (p ≤ 0.001). There was no significant relationship between surgical approach and aneurysm projection or A2 plane (p = 0.157 &p = 0.318). CONCLUSION: Regardless of whether the A2 plane is open or closed, the A1 dominant side is still a better choice for accessing AComA aneurysms to avoid dangerous premature bleeding.

7.
J Colloid Interface Sci ; 564: 143-154, 2020 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-31911220

RESUMO

Novel BiOCl/TiO2/clinoptilolite composite was prepared via a homogeneous precipitation and calcination-crystallization process in this study. Through structural design and spatial assembly, the ternary composite based on clinoptilolite layer as well as the TiO2 nano particles and BiOCl nano sheets was successfully synthesized. The prepared composite was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high resolution transmission electron microscope (HRTEM), X-ray photoelectron spectroscopy (XPS), N2 isothermal adsorption-desorption test and electrochemical measurement, respectively. Compared with bare photocatalysts (i.e. TiO2 and BiOCl), the composite exhibited enhanced photoactivity under visible light irradiation towards rhodamine B (RhB). Based on the pseudo-first-order kinetic constants, the photocatalytic performance of BiOCl/TiO2/clinoptilolite composite is around 48 and 11 times higher than that of pure TiO2 and BiOCl, respectively. The enhanced photocatalytic properties of the composite should be attributed to the improved adsorption ability and the formation of the n-p heterojunction between BiOCl and TiO2, which is beneficial to establish the rapid adsorption-desorption system and promote the efficient separation of the photogenerated electron-hole pairs. The excellent visible-light-driven performance makes the BiOCl/TiO2/clinoptilolite composite possible to be utilized in the field of industrial wastewater treatment.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31935966

RESUMO

Ground and environmental vibrations induced by high dam flood discharge from the Xiangjiaba hydropower station (XHS) has significant adverse effects on nearby building safety and the physical and mental health of surrounding residents. As an effective approach to simulate the flow-induced vibration of hydraulic structures, the hydro-elastic experiment approach has been extensively applied and researched by Chinese scholars, but the relevant systematic research is rarely reported in international journals. Firstly, the hydraulic and structural dynamic similarity conditions that should be satisfied by the hydro-elastic model are briefly reviewed and derived. A hydro-elastic model of the XHS was further constructed using self-developed high-density rubber, and the vibration isolation system (including open trenches and flexible connects) was applied to avoid the external disturbances of pump operation, vehicle vibration and other experiments in the laboratory. Based on the data of model and prototype dynamic tests, a back propagation (BP) neural network was established to map the acceleration of the physical model to the ground in the prototype. In order to reduce the ground vibration, experiments were carried out to meticulously evaluate the ground vibration intensity under more than 600 working conditions, and the optimal operation scheme under different discharge volumes is presented here in detail. According to the prototype test data in 2013, 2014, and 2015, ground vibrations were significantly reduced by applying the presented optimal operation principle which indicates that the presented hydro-elastic approach and the vibration attenuation operation scheme were effective and feasible.

9.
BJU Int ; 125(2): 314-321, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30924591

RESUMO

OBJECTIVE: To report our experience in the diagnosis, minimally invasive treatment, and composition of seminal vesicle calculi (SVC). PATIENTS AND METHODS: In the present study, we evaluated 20 patients who were admitted to our hospital from January 2013 to January 2018. All the patients were diagnosed with intractable haematospermia and SVC. The diagnosis was further confirmed by seminal vesiculoscopy. SVC were removed by basket extraction; with larger SVC fragmented by holmium laser before extraction. Scanning electron microscopy, X-ray diffraction, and infrared spectroscopy were used to determine the SVC composition. RESULTS: All operations were completed successfully without surgical complications. SVC were mostly composed of hydroxyapatite and protein, suggesting that they were produced by infections. CONCLUSIONS: Seminal vesiculoscopy is a simple, minimally invasive technique that can be used for diagnostic confirmation and treatment of seminal vesiculitis with SVC. This study improves our understanding of SVC and provides a theoretical basis for the prevention of postoperative recurrence of SVC.

10.
Opt Express ; 27(26): 37680-37694, 2019 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-31878545

RESUMO

Optically massive trapping of the moisture in the air into an adjacent surface is a potential technique in the fields of bacterial adhesion and microfluidic generation, which is quite important to the development of LN-based biological lab-on-chips. Here we demonstrate on a LiNbO3:Fe substrate the visible-light-assisted condensation of the water vapor in a flowing stream created by an ultrasonic atomizer. Through analyzing the dynamic processes of the visible-light-assisted water condensation at different illumination intensities, it is found that the extent of the water condensation, the bending angle of water vapor trails and the interaction range of the condensation effect are highly dependent on the illumination intensity. According to these findings and the simulated trajectories of the water vapor stream at different illumination intensities, we propose that this visible-light-assisted water condensation is an aggregation process of tiny water droplets driven by the dielectrophoretic interaction of inhomogeneous photovoltaic field and also an electrostatic screening course of photovoltaic charges through the charged evaporation of condensed water. The prolonged condensation of water vapor after a high-intensity illumination and that of oil vapor at a super-low evaporation rate are also studied, and the agreement between the simulation and experimental results reinforces the above mechanism. The reported technique, employing the inexpensive, safe-for-cell visible laser beam, is quite convenient for the controllable generation of various biological microdroplets, and thus it is promising for the microfluidic functionality integration of LN-based biological lab-on-chips.

11.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4249-4256, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872706

RESUMO

In this study,liquiritigenin sulfonation was characterized using recombinant human sulfotransferases( SULTs). The chemical structure of liquiritigenin sulfate was determined by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry( UPLC-Q-TOF-MS/MS). Then model fitting and parameter estimation were performed using the Graphpad Prism V5 software. Various SULT enzymes( SULT1 A1,1 A2,1 A3,1 B1,1 C2,1 C4,1 E1 and 2 A1) were able to catalyze the formation of liquiritigenin-7-O-sulfate. Sulfonation of liquiritigenin-7-hydroxy( 7-OH) by these eight SULT enzymes consistently displayed the classical Michaelis-Menten profile. According to the intrinsic clearance( CLint) value,the sulfonation rates of liquiritigenin-7-OH by expressed SULT enzymes followed the following rank order: SULT1 C4 > SULT1 A3 > SULT1 E1 > SULT1 A1 > SULT1 A2 > SULT1 B1 >SULT1 C2>SULT2 A1. Further,liquiritigenin-7-O-sulfonation was significantly correlated with the SULT1 A3 protein levels( P<0. 05).Then,human embryonic kidney( HEK) 293 cells over expressing SULT1 A3( named as HEK-SULT1 A3 cells) were conducted. As a result,liquiritigenin-7-O-sulfate( L-7-S) was rapidly generated upon incubation of the cells with liquiritigenin. Consistent with SULT1 A3,sulfonation of liquiritigenin-7-OH in HEK-SULT1 A3 cells also followed the Michaelis-Menten kinetics. The derived Vmaxvalues was( 0. 315±0. 009) µmol·min-1·g-1,Kmwas( 7. 04±0. 680) µmol·L-1,and CLintwas( 0. 045±0. 005) L·min-1·g-1. Moreover,the sulfonation characters of liquiritigenin( 7-OH) in SULT1 A3 were strongly correlated with that in HEK-SULT1 A3 cells( P<0. 001).The results indicated that HEK-SULT1 A3 cells have shown the catalytic function of SULT1 A3 enzymes. In conclusion,liquiritigenin was subjected to efficient sulfonation,and SULT1 A3 enzyme plays an important role in the sulfonation of liquiritigenin-7-OH. Significant sulfonation should be the main reason for the low bioavailability of liquiritigenin. In addition,HEK-SULT1 A3 cells were conducted and successfully used to evaluate liquiritigenin sulfonation,which will provide an appropriate tool to accurately depict the sulfonation disposition of liquiritigenin in vivo.


Assuntos
Flavanonas/metabolismo , Espectrometria de Massas em Tandem , Arilsulfotransferase , Humanos
12.
Microbiome ; 7(1): 150, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31757223

RESUMO

Following publication of the original article [1], the authors reported an error in the Additional file 1.

13.
Environ Sci Technol ; 53(23): 13878-13887, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31697480

RESUMO

The good performance of base metal catalysts for the electrooxidation of organic pollutants has attracted great attention. However, base metal phosphides for electrooxidation are seldom studied owing to the sluggish water adsorption and dissociation dynamics, which will hinder the production of the sorbed hydroxyl radicals (M(•OH)) and thus inhibit the electrooxidation of organic pollutants. Herein, we proposed a universal strategy to improve the electrooxidation capability of metal phosphides by modulating the surface electron densities. The electron interactions between cobalt (Co) and phosphorus (P) are modulated after iron doping, resulting in more positively charged Co and more negatively charged P, which can promote the adsorption and activation of water molecules and produce large quantities of M(•OH). Meanwhile, the experimental results show that the iron-modulated Fe0.53Co0.47P nanosheet arrays exhibit higher removal efficiency of tetracycline than the boron-doped diamond and Pt anode at low current intensity. Based on experimental results and density functional theory + U calculations (DFT + U), it is found that Fe0.53Co0.47P has lower barrier (0.45 eV) to form the sorbed hydroxyl radicals (M(•OH)) and higher overpotential to produce O2 than its counterparts, suggesting that Fe0.53Co0.47P can produce more M(•OH) instead of O2. The above results highlighted the feasibility of these base metal phosphides for electrooxidation for advanced water purification.


Assuntos
Poluentes Ambientais , Poluentes Químicos da Água , Cobalto , Elétrons , Oxirredução
14.
Fish Physiol Biochem ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31701282

RESUMO

An 8-week feeding trial was conducted to investigate the effects of enzyme-treated soy protein (ETSP) supplementation in low protein diets on growth performance as well as flesh sensory quality and healthiness in on-growing grass carp. A total of 540 on-growing grass carp (initial average weight 325.72 ± 0.60 g) were fed six diets, which included a normal protein diet (28% crude protein) and five low protein diets (26% crude protein) supplemented with graded levels of ETSP (0.0, 0.8, 1.2, 1.6, and 2.0%). The results showed that reducing dietary protein by 2% decreased percentage weight gain, feed intake, and flesh flavor (aspartic acid, glutamic acid, histidine, and 5'-inosinic acid contents) and healthiness-related indices (linolenic acid (LA) and docosahexaenoic acid (DHA) contents and polyunsaturated fatty acids to saturated fatty acids ratio). Under the condition of reducing dietary protein by 2%, 0.8-1.2% ETSP supplementation restored above parameters to levels equal or superior to those in 28% crude protein diet group. Although reducing dietary protein by 2% did not deteriorate flesh tenderness and juiciness, 0.8-1.2% ETSP supplementation in low protein diets also improved the two indices compared with 28% crude protein diet. Moreover, ETSP-improved flesh quality was partly related to increased muscle antioxidant enzymes activities and their mRNA levels. In addition, ESTP-enhanced antioxidant enzyme mRNA levels were partly associated with the upregulation of NF-E2-related factor 2 (Nrf2) and target of rapamycin (TOR) signaling. Collectively, 0.8-1.2% ETSP supplementation in low protein diets improved growth performance as well as flesh sensory quality and healthiness in on-growing grass carp.

15.
Small ; 15(50): e1905050, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31721434

RESUMO

Upconversion near-infrared (NIR) fluorescent carbon dots (CDs) are important for imaging applications. Herein, thermally activated upconversion photoluminescence (UCPL) in the NIR region, with an emission peak at 784 nm, which appears under 808 nm continuous-wave laser excitation, are realized in the NIR absorbing/emissive CDs (NIR-CDs). The NIR-CDs are synthesized by microwave-assisted exfoliation of red emissive CDs in dimethylformamide, and feature single or few-layered graphene-like cores. This structure provides an enhanced contact area of the graphene-like plates in the core with the electron-acceptor carbonyl groups in dimethylformamide, which contributes to the main NIR absorption band peaked at 724 nm and a tail band in 800-850 nm. Temperature-dependent photoluminescence spectra and transient absorption spectra confirm that the UCPL of NIR-CDs is due to the thermally activated electron transitions in the excited state, rather than the multiphoton absorption process. Temperature dependent upconversion NIR luminescence imaging is demonstrated for NIR-CDs embedded in a polyvinyl pyrrolidone film, and the NIR upconversion luminescence imaging in vivo using NIR-CDs in a mouse model is accomplished.

16.
Nat Chem ; 11(12): 1151-1157, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31712613

RESUMO

Semiconductor quantum-well structures and superlattices are key building blocks in modern optoelectronics, but it is difficult to simultaneously realize defect-free epitaxial growth while fine tuning the chemical composition, layer thickness and band structure of each layer to achieve the desired performance. Here we demonstrate the modulation of the electronic structure-and consequently the optical properties-of organic semiconducting building blocks that are incorporated between the layers of perovskites through a facile solution processing step. Self-aggregation of the conjugated organic molecules is suppressed by functionalization with sterically demanding groups and single crystalline organic-perovskite hybrid quantum wells (down to one-unit-cell thick) are obtained. The energy and charge transfers between adjacent organic and inorganic layers are shown to be fast and efficient, owing to the atomically flat interface and ultrasmall interlayer distance of the perovskite materials. The resulting two-dimensional hybrid perovskites are very stable due to protection given by the bulky hydrophobic organic groups.

17.
Epigenomics ; 11(15): 1693-1715, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31650864

RESUMO

Aim: To analyze the m6A methylome of osteosarcoma stem cells (OSCs). Materials & methods: Chemoresistant OSCs were enriched by doxorubicin treatment. Expression of m6A-related enzymes was detected by quantitative real-time-PCR and western blot. MeRIP-seq and RNA-seq were performed to identify differences in m6A methylation and gene expression. Data analysis was conducted to explore the modified genes and their clinical significance. Results: Three m6A-related enzymes were altered in OSCs. Differentially methylated genes were enriched in some pathways regulating pluripotency of stem cells. The expression of several candidate genes were found consistent with that in GSE33458 dataset, and associated with poor prognosis in osteosarcoma patients. Conclusion: m6A may play a role in the emergence and maintaining of OSCs and affect the prognosis.

18.
Fungal Genet Biol ; 133: 103282, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31629081

RESUMO

Candida albicans is an important opportunistic fungal pathogen, and hyphal polarized growth is critical for its invasive infection to the host. Both the vacuolar transient receptor potential (TRP) Ca2+ channel Yvc1 and the NADPH oxidase Fre8-governed reactive oxygen species (ROS) gradient are involved in hyphal development, but the relationship between Yvc1 and Fre8 during hyphal polarized growth remains to be investigated. Herein, we found that deletion of YVC1 led to dispersed distribution of ROS along the germ tube, while it was concentrated at the hyphal tip in WT cells. Moreover, Fre8 localization was altered as YVC1 was disrupted. Besides, similar to deletion of YVC1, addition of the Ca2+ chelating agent EGTA caused depolarization of Fre8-GFP in the wild-type cells, indicating the critical role of Yvc1-maintained Ca2+ gradient in polarized distribution of Fre8-GFP and consequent disruption of tip ROS gradient. By constructing a series of GFP-tagged polarized growth-related proteins, including Bud6, Exo70 and Lifeact, we found that these proteins, similar to Fre8 and ROS, had depolarized localization in yvc1Δ/Δ. Thus, our work provides a mechanic explanation of Yvc1-governed and ROS-related hyphal polarized growth, and shed a novel light on the role of Ca2+ signaling in maintenance of redox homeostasis and morphogenesis in the fungal pathogens.

19.
Nat Commun ; 10(1): 4579, 2019 10 08.
Artigo em Inglês | MEDLINE | ID: mdl-31594926

RESUMO

Rheumatoid arthritis (RA) is a chronic inflammatory disorder characterized by progressive bone erosion. Leflunomide is originally developed to suppress inflammation via its metabolite A77 1726 to attenuate bone erosion. However, distinctive responsiveness to Leflunomide is observed among RA individuals. Here we show that Leflunomide exerts immunosuppression but limited efficacy in RA individuals distinguished by higher serum C-reactive protein (CRPHigher, CRPH), whereas the others with satisfactory responsiveness to Leflunomide show lower CRP (CRPLower, CRPL). CRP inhibition decreases bone erosion in arthritic rats. Besides the immunomodulation via A77 1726, Leflunomide itself induces AHR-ARNT interaction to inhibit hepatic CRP production and attenuate bone erosion in CRPL arthritic rats. Nevertheless, high CRP in CRPH rats upregulates HIF1α, which competes with AHR for ARNT association and interferes Leflunomide-AHR-CRP signaling. Hepatocyte-specific HIF1α deletion or a HIF1α inhibitor Acriflavine re-activates Leflunomide-AHR-CRP signaling to inhibit bone erosion. This study presents a precision medicine-based therapeutic strategy for RA.

20.
Small ; 15(46): e1903628, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31577387

RESUMO

It is hard for current radionuclide therapy to render solid tumors desirable therapeutic efficacy owing to insufficient tumor-targeted delivery of radionuclides and severe tumor hypoxia. In this study, a biocompatible hybrid protein nanoreactor composed of human serum albumin (HSA) and catalase (CAT) molecules is constructed via glutaraldehyde-mediated crosslinking. The obtained HSA-CAT nanoreactors (NRs) show retained and well-protected enzyme stability in catalyzing the decomposition of H2 O2 and enable efficient labeling of therapeutic radionuclide iodine-131 (131 I). Then, it is uncovered that such HSA-CAT NRs after being intravenously injected into tumor-bearing mice exhibit efficient passive tumor accumulation as vividly visualized under the fluorescence imaging system and gamma camera. As the result, such HSA-CAT NRs upon tumor accumulation would significantly attenuate tumor hypoxia by decomposing endogenous H2 O2 produced by cancer cells to molecular oxygen, and thereby remarkably improve the therapeutic efficacy of radionuclide 131 I. This study highlights the concise preparation of biocompatible protein nanoreactors with efficient tumor homing and hypoxia attenuation capacities, thus enabling greatly improved tumor radionuclide therapy with promising potential for future clinical translation.

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