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1.
Food Chem ; 368: 130814, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34428689

RESUMO

Furfural compounds are produced during infant formula production when heat treatment is involved. In this study, a robust method was established for determining potential and free furfural compounds (furfural, 5-methyl-2-furfural, 2-acetylfuran and 5-hydroxymethyl-2-furfural) using a modified QuEChERS technique coupled with GC-MS/MS. Further, 36 samples of two batches, covering the whole infant formula production chain were analyzed by the method to investigate how furfurals evolved during process. Then risk assessment was conducted using the Toxtree and T.E.S.T. software and evaluated by hazard quotient. Results showed the contents of bound and free 5-hydroxymethyl-2-furfural demonstrated largest increase during spray-drying (6-11 times) and homogenization stages (12-33 times), respectively. As the sum up of bound and free 5-hydroxymethyl-2-furfural, potential 5-hydroxymethyl-2-furfural was found can cause safety risks in the production chain due to the high hazard quotient value (3.11), indicating it should be controlled in homogenization and spray-drying stages.


Assuntos
Furaldeído , Fórmulas Infantis , Furaldeído/análise , Humanos , Medição de Risco , Espectrometria de Massas em Tandem
2.
Transl Pediatr ; 10(4): 715-722, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34012821

RESUMO

Background: Mediastinal neuroblastoma (NB) can invade the spinal canal and result in spinal cord compression. Some patients go on to develop severe spinal deformities after decompression of the spinal cord. The optimal therapeutic strategy for mediastinal NB with intraspinal extension is still unclear. Our study is to assess the therapeutic strategies for such patients. Methods: A total of 77 patients suffered mediastinal tumors with intraspinal extension between March 2015 and Aug 2019 were enrolled in the study. According to the primary therapy, NB were classified into 4 groups: chemotherapy, video-assisted thoracoscopic surgery (VATS)/thoracotomy, neurosurgical decompression, and a combined thoracic-neurosurgical approach. Clinical features, including patient demographics, neurologic recovery and survival rate, were assessed. Results: Among the 77 patients suffered mediastinal tumors with intraspinal extension, neurological symptoms were present in 44 patients. Neurological deficits improved in 76.5% of patients who underwent neurosurgical intervention and 50% of the other patients (P=0.094). Compression manifestations of ≤4 weeks duration showed an improved outcome compared to a longer compression time, with complete recovery of neurological function in 60% of patients versus 28.6% for patients with a longer symptom duration (P=0.04). NB constituted 49.4% of the 77 patients. An overall survival rate of 90.0%±9.5% was achieved for patients in the combined thoracic-neurosurgical group, 59.5%±15.0% in the thoracotomy group, 40.0%±29.7% in laminectomy group, and 37.0%±20.2% in the chemotherapy group. Complete regression of the tumor was demonstrated in 80% of combined group, which was greater than that of patients in the other groups (P=0.001). Conclusions: Neurological recovery was correlated with the type of initial treatment and the duration of neurological symptoms. Mediastinal NB with intraspinal extension can be effectively managed with a combined neurosurgical and thoracic surgical approach.

3.
Bioengineered ; 12(1): 1299-1310, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-33904381

RESUMO

Differentiated thyroid carcinoma (DTC) is one of the most common malignant tumors. Increasing evidence indicates that centromere protein K(CENPK) may play a key role in promoting carcinogenesis. The expression, biological functions, and clinical significance of CENPK in DTC are still unclear. The CENPK expression in the DTC specimen was confirmed using quantitative real-time PCR and Western blot. The expression of CENPK was silenced and promoted by lentivirus-mediated transfection with shRNA sequences or CENPK plasmid targeting CENPK in TPC1 and FTC-133 cells, respectively. Colony formation, Cell Counting Kit-8 (CCK-8), Transwell invasion, and scratch assays were performed to assess the malignant biological properties of FTC-133 and TPC1 cells. Tumorigenicity assay was performed using C57BL/6 mice to explore the influence of CENPK on the growth of TPC1. The present work suggested that the expression of CENPK remarkably increased in follicular thyroid cancer and papillary thyroid cancer  tissue samples at the mRNA level. Immunohistochemical staining also showed consistent results at the protein level. In addition, CENPK mRNA expression level showed great value in diagnosis of DTC. Knockdown of CENPK significantly inhibited the invasion and migration of TPC1 and FTC-133 cells. In contrast, CENPK overexpression promoted invasion and migration of TPC1 and FTC-133 cells. Knockdown and overexpression of CENPK showed consistent effect on DTC tumor growth and expression of Ki-67 invivo. Our results indicated that CENPK was evidently upregulated in DTC. Knocking down CENPK suppressed TPC1 cell proliferation, invasion and migration. Targeting the CENPK may be anovel therapeutic method for DTC.


Assuntos
Movimento Celular/genética , Proteínas de Ligação a DNA/genética , Proteínas Nucleares/genética , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/patologia , Animais , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos Nus , Invasividade Neoplásica , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ensaio Tumoral de Célula-Tronco , Regulação para Cima/genética
4.
Food Res Int ; 140: 109899, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33648201

RESUMO

Peanuts in China are heavily contaminated with aflatoxin, which pose a threaten to human health. To compare the dietary exposure risk of aflatoxins (AFT) in peanuts and peanut oil in different areas of China, the spatial distribution of AFT contamination levels in peanuts and peanut oil from different areas was analyzed. The dietary exposure was calculated by simple distributed risk assessment method before characterizing the health risk using both the margin of exposure (MOE) approach proposed by the European Food Safety Authority (EFSA) and the quantitative liver cancer risk approach proposed by the Joint Food and Agricultural Organization/World Health Organization (FAO/WHO) Expert Committee on Food Additives (JECFA). The results showed that the AFT content in peanuts and peanut oil was high with agglomeration in several provinces of East and South China under a subtropical temperate monsoon climate, and the AFT contamination in peanut oil was more substantial than peanuts. On average, the estimated dietary exposure to AFT from the total of peanuts and peanut oil for Chinese general population ranged from 1.776 to 1.940 ng/kg bw/day (LB-UB), from which the MOE values of 88-96 (UB-LB) and liver cancer risk of 0.055-0.060 cases/100,000 persons/year (LB-UB) were calculated. As for different areas in China, the mean AFT exposure ranged between 0.000 and 17.270 ng/kg bw/day. Moreover, the corresponding health risk was estimated at 10-868759 MOE values and 0.000-0.851 liver cancer cases/100,000 persons/year. Guangdong, Fujian and Jiangxi provinces were at a higher risk rank. The liver cancer risk of AFT exposure from peanuts and peanut oil was far below all-cause liver cancer incidence (18.0 cases/100,000 persons/year) in China, but several areas with relatively high risk should be of concern. Compared with other age groups, children aged 2-6 years should be paid more attention because they have the highest AFT exposure level.


Assuntos
Aflatoxinas , Aflatoxina B1/análise , Aflatoxinas/análise , Arachis , Criança , Pré-Escolar , China/epidemiologia , Exposição Dietética , Contaminação de Alimentos/análise , Humanos , Óleo de Amendoim , Análise Espacial
5.
Zhongguo Zhong Yao Za Zhi ; 45(22): 5403-5411, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33350199

RESUMO

As a single-cell organism, Plasmodium has a large and complex metabolic network system. There is a close relationship between various metabolic pathways to maintain the transformation of Plasmodium's own energy and substances. Plasmodium energy metabolism pathways mainly include glycolysis and oxidative phosphorylation. Among them, Plasmodium at the erythrocytic stage takes glycolysis as the main energy supply method, and less energy is generated by oxidative phosphorylation. In addition, the two carbon metabolism pathways closely relating to energy metabolism are the tricarboxylic acid(TCA) cycle pathway and glutamate metabolism pathway. As the core of metabolism, the TCA cycle connects glycolysis and glutamate metabolism; glutamate metabolism, as the main carbon metabolism pathway, also participates in various metabolic pathways, such as pyrimidine metabolism, porphyrin metabolism, and protein biosynthesis. This article reviews the energy metabolism pathways of Plasmodium and carbon metabolism pathways that are closely related to energy metabolism, in order to deepen the understanding of the energy metabolism of Plasmodium at the erythrocytic stage, and then provide the theoretical basis and references for studying the mechanisms of action and the drug resistance of antimalarial drugs.


Assuntos
Metabolismo Energético , Plasmodium , Ciclo do Ácido Cítrico , Glicólise , Fosforilação Oxidativa
6.
Eur J Radiol ; 132: 109302, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33007518

RESUMO

PURPOSE: To compare virtual monoenergetic images (VMIs) with conventional polyenergetic images (PI) of Dual-layer spectral detector CT angiography (DLCTA) in plaque burden assessment and attenuation measurement of carotid atherosclerotic plaques. METHODS: Supra-aortic DLCTA imaging of thirty patients (8 female, mean ages 63.1 ±â€¯7.5 years) were respectively reviewed. Lumen area, wall area, and calcified area of plaques were outlined and recorded. Normalized wall index (NWI) was calculated for plaque burden and compared between PI and different VMIs. The attenuation of the non-calcified, calcified area of the plaques, sternocleidomastoid muscle (SCM), as well as Z effective values were measured and compared. RESULTS: Fifty carotid plaques (27 left, 23 right) of thirty patients were analyzed. The average values of lumen, wall, calcified areas and NWI on PI were 34.50 ±â€¯20.57mm2, 47.61 ±â€¯19.94 mm2, 5.25 mm2 (1.35- 51.86 mm2), and 0.59 ±â€¯0.16 respectively. No significant difference was found in the lumen area (p = 0.314), wall area (p = 0.600), and NWI (p = 0.980) between different VMIs and PI. A significant difference was found in the calcified area between VMIs and PI (p = 0.009). Attenuations of non-calcified and calcified components in carotid plaques were comparable to PI for 50-120 keV (all: p > 0.05) and 60-120 keVs (all p > 0.05), respectively. Z Effective values for non-calcified, calcified and SCM were 7.67 ±â€¯0.42, 11.70 ±â€¯1.22, and 7.45 ±â€¯0.12, respectively. CONCLUSIONS: Carotid plaque burden assessment was comparable between PI and VMIs at 40-120 keVs. Attenuations of non-calcified components in carotid plaques were comparable to PI for 50-120 keV VMIs of DLCTA. VMIs might provide more information on carotid plaque features.


Assuntos
Placa Aterosclerótica , Idoso , Artérias Carótidas/diagnóstico por imagem , Angiografia por Tomografia Computadorizada , Feminino , Humanos , Pessoa de Meia-Idade , Placa Aterosclerótica/diagnóstico por imagem , Estudos Retrospectivos
7.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 36(8): 693-698, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32958125

RESUMO

Objective To investigate the effects of isochlorogenic acid A on the proliferation, migration, invasion and apoptosis of MH7A cells. Methods Following by the induction of 20 µg/L TNF-α in vitro, different concentrations of isochlorogenic acid A (0, 0.04, 0.09, 0.13, 0.16, 0.20 µg/µL) were added into MH7A cells. The proliferation of MH7A cells was detected by CCK-8 assay, and the invasion and migration were observed by TranswellTM assay. The levels of cellular caspase-3, ROS and MMP3 in the MH7A cells were detected by corresponding kits. BAX and Bcl2 expression of MH7A cells were tested by immunofluorescence cytochemistry. Results The proliferation, invasion and migration activity were enhanced significantly and the apoptotic activity was reduced in MH7A cells induced by 20 µg/L TNF-α. Compared with TNF-α in vitro induction, isochlorogenic acid A significantly inhibited the proliferation, invasion, migration and MMP3 secretion of MH7A cells, increased ROS release and promoted MH7A apoptosis. Conclusion Isochlorogenic acid A can inhibit proliferation, invasion, migration and promoted apoptosis of MH7A cells.


Assuntos
Apoptose , Ácido Clorogênico/análogos & derivados , Fator de Necrose Tumoral alfa , Apoptose/efeitos dos fármacos , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ácido Clorogênico/farmacologia , Humanos , Fator de Necrose Tumoral alfa/imunologia
8.
Int Immunopharmacol ; 82: 106326, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32135490

RESUMO

OBJECTIVE: Pristimerin is known to have anti-cancer and anti-inflammatory activities; however, its therapeutic mechanism has not been described. In this study, to investigate the therapeutic mechanism of pristimerin, we examined the effect of pristimerin on TNF-α-induced endothelial inflammatory response both in vitro and in vivo. METHODS: Leukocyte-endothelium Adhesion Assay was use to evaluate the endothelial cell-monocyte interaction. Western blotting was used to confirm protein expression. NF-κB p65 nuclear translocation in endothelial cells was detected using immunofluorescent microscopy. In vivo leukocyte infiltration was evaluated using acute lung inflammation model. RESULTS: Pristimerin profoundly inhibited TNF-α-induced adhesion of monocytes to human endothelial cells and the leukocyte transmigration. Pristimerin dramatically inhibited the expression of TNF-α-induced endothelial adhesion molecules (intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1)) and the pro-inflammatory cytokine (IL-6, IL-8 and monocyte chemoattractant protein-1 (MCP-1)). Pristimerin suppressed the penetration of the leukocyte in the acute lung injury mice model. Furthermore, pristimerin also suppressed the TNF-α-activated Nuclear factor kappa B (NF-κB) activation. CONCLUSIONS: Pristimerin has the anti-inflammatory properties in endothelial cells, at least in part, through the suppression of NF-κB activation, which may have a potential therapeutic effects for inflammatory vascular diseases.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31947699

RESUMO

OBJECTIVE: This study was conducted to evaluate the acute and subchronic toxicity of gardenia yellow, a natural colorant widely used in China and other Asian countries. An acute toxicity test was performed in S-D rats of both genders and the lethal dose (LD50) of per oral gardenia yellow was estimated to be more than 15.0 g/kg·bw. In the subchronic study, gardenia yellow was orally administered to rats by gavage at doses of 0, 0.50, 1.50 and 4.50 g/kg·bw/day for 90 days followed by a recovery period of 28 days. No appreciable toxic-related changes were observed in the 0.50 g/kg·bw/day group. When the animals received gardenia yellow at 1.50 g/kg·bw/day or more, body weight loss was observed, and pigments began to deposit in several vital organs, resulting in significant changes of several hematological and biochemical indicators related to the nutritional status of the body, liver and kidney function, more severe in the high dose group. In the recovery period, the alterations of the clinical symptoms and parameters were relieved a lot. Based on the results of the current study, the no observed adverse effect level (NOAEL) of gardenia yellow E500 in rats was set to be 0.50 g/kg·bw/day.


Assuntos
Gardenia/toxicidade , Extratos Vegetais/toxicidade , Administração Oral , Animais , Peso Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Dose Letal Mediana , Fígado/efeitos dos fármacos , Masculino , Nível de Efeito Adverso não Observado , Ratos , Ratos Sprague-Dawley , Testes de Toxicidade Aguda , Testes de Toxicidade Subcrônica
10.
Artif Cells Nanomed Biotechnol ; 48(1): 8-14, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31852248

RESUMO

Osteoarthritis is a common type of degenerative joint disease. Inflammation-related chondrocyte senescence plays a major role in the pathogenesis of osteoarthritis. Omentin-1 is a newly identified anti-inflammatory adipokine involved in lipid metabolism. In this study, we examined the biological function of omentin-1 in cultured chondrocytes. The presence of omentin-1 potently suppresses IL-1ß-induced cellular senescence as revealed by staining with senescence-associated beta-galactosidase (SA-ß-Gal). At the cellular level, omentin-1 attenuates IL-1ß-induced G1 phase cell-cycle arrest. Mechanistically, we demonstrate that omentin-1 reduced IL-1ß-induced expression of senescent factors including caveolin-1, p21, and PAI-1 as well as p53 acetylation through ameliorating SIRT1 reduction. Notably, silencing of SIRT1 abolishes IL-1ß-induced senescence along with the induction of p21 and PAI-1, suggesting that the action of omentin-1 in chondrocytes is dependent on SIRT1. Collectively, our results revealed the molecular mechanism through which the adipokine omentin-1 exerts a beneficial effect, thereby protecting chondrocytes from senescence. Thus, omentin-1 could have clinical implication in the treatment of osteoarthritis.


Assuntos
Adipocinas/farmacologia , Senescência Celular/efeitos dos fármacos , Condrócitos/citologia , Condrócitos/efeitos dos fármacos , Interleucina-1beta/farmacologia , Caveolina 1/genética , Linhagem Celular Tumoral , Inibidor de Quinase Dependente de Ciclina p21/genética , Citoproteção/efeitos dos fármacos , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Humanos , Inibidor 1 de Ativador de Plasminogênio/genética , Sirtuína 1/metabolismo , Ativação Transcricional/efeitos dos fármacos
11.
Artigo em Inglês | MEDLINE | ID: mdl-31248171

RESUMO

Objective: To investigate the levels of a deoxynivalenol (DON) biomarker in the urine of subjects living in two China provinces with different geographic locations and dietary patterns, and estimate their dietary DON exposures and health risks. Methods: First morning urine samples were collected on three consecutive days from 599 healthy subjects-301 from Henan province and 298 from Sichuan province-to analyze the total DON concentrations (tDON) after ß-glucuronidase hydrolysis using a high-performance liquid chromatography tandem mass spectrometry-based method. The consumption of cereal foods in the previous 24 h before each urine collection was recorded using a duplicate diet method. DON exposure levels were estimated based on the urinary tDON concentrations. Results: Total DON were detected in 100% and 92% of the urine samples from Henan and Sichuan, respectively. Mean urinary tDON concentrations were 52.83 ng/mL in Henan subjects and 12.99 ng/mL in Sichuan subjects, respectively. The tDON levels were significantly higher in the urine of Henan subjects than that of the Sichuan subjects (p < 0.001). Urinary tDON levels were significantly different among age groups in both areas (Henan: p < 0.001; Sichuan: p = 0.026) and were highest in adolescents aged 13-17 years, followed by children aged 7-12 years. Based on the DON biomarker and exposure conversion reported by the European Food Safety Authority (EFSA), the mean estimated dietary intakes of DON were 1.82 µg/kg bw/day in Henan subjects and 0.45 µg/kg bw/day in Sichuan subjects. A total of 56% of Henan subjects and 12% of Sichuan subjects were estimated to exceed the PMTDI of 1 µg/kg bw/day. Consistent with urinary tDON levels, the highest estimated dietary DON intakes were also in children and adolescents aged 7-17 years. For all kinds of wheat-based foods except dumplings, the consumptions were significantly higher in Henan than those in Sichuan. The mean consumption of steamed buns was 8.4-fold higher in Henan (70.67 g/d) than that in Sichuan (8.45 g/d). The mean consumption of noodles in Henan (273.91 g/d) was 3.6-fold higher than that in Sichuan (75.87 g/d). Conclusions: The levels of urinary DON biomarker and the estimated dietary DON intakes in Henan province were high and concerning, especially for children and adolescents. The overall exposure level of Sichuan inhabitants was low.


Assuntos
Monitoramento Biológico/métodos , Biomarcadores/urina , Exposição Dietética/efeitos adversos , Grão Comestível/química , Contaminação de Alimentos/análise , Tricotecenos/análise , Tricotecenos/química , Adolescente , Fatores Etários , Criança , Pré-Escolar , China , Cromatografia Líquida de Alta Pressão , Feminino , Geografia , Humanos , Lactente , Masculino
12.
J Zhejiang Univ Sci B ; 20(6): 476-487, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31090273

RESUMO

Life may have begun in an RNA world, which is supported by increasing evidence of the vital role that RNAs perform in biological systems. In the human genome, most genes actually do not encode proteins; they are noncoding RNA genes. The largest class of noncoding genes is known as long noncoding RNAs (lncRNAs), which are transcripts greater in length than 200 nucleotides, but with no protein-coding capacity. While some lncRNAs have been demonstrated to be key regulators of gene expression and 3D genome organization, most lncRNAs are still uncharacterized. We thus propose several data mining and machine learning approaches for the functional annotation of human lncRNAs by leveraging the vast amount of data from genetic and genomic studies. Recent results from our studies and those of other groups indicate that genomic data mining can give insights into lncRNA functions and provide valuable information for experimental studies of candidate lncRNAs associated with human disease.


Assuntos
Mineração de Dados , Genômica , RNA Longo não Codificante/fisiologia , Transtorno do Espectro Autista/genética , Humanos , Aprendizado de Máquina , RNA Longo não Codificante/análise , Máquina de Vetores de Suporte
13.
Artigo em Inglês | MEDLINE | ID: mdl-31100047

RESUMO

The aim of the study was to assess the dietary intake of caramel colours and their by-products 4-methylimidazole (4-MEI) and 2-acetyl-4-tetrahydroxybutylimidazole (THI) for the Chinese population. Based on the typical and maximum reported use levels of caramel colours in 15 food categories, the dietary intakes of combined and single-class caramel colours of Classes I, III and IV were estimated with the food consumption data from the China National Nutrient and Health Survey. Using the mean values of 4-MEI and THI contents in Class III and Class IV Caramel colour samples, the exposures to 4-MEI and THI from dietary caramel colours were derived. The results showed that the combined and individual average dietary caramel colour intakes for the Chinese population of different age groups were estimated to be 232-60.3 mg kg-1 bw day-1 for combined caramels, 5.9-29.2 mg kg-1 bw day-1 for Class I, 7.7-29.6 mg kg-1 bw day-1 for Class III, 21.2-54.3 mg kg-1 bw day-1 for Class IV, which were far below the group acceptable daily intake (ADI) and respective ADIs. The combined intake of 4-MEI from Class III and IV caramel colours was estimated to be 3.8-5.2 µg kg-1 bw day-1 on average, and 12.9-27.1 µg kg-1 bw day-1 at 95th-97.5th percentile for the general population. The anticipated exposure to THI from Class III caramel colours was estimated to be 0.1-0.3 µg kg-1 bw day-1 on average and 0.5-1.7 µg kg-1 bw day-1 at 95th-97.5th percentile for the general population. The dietary caramel colours intakes and the exposures to 4-MEI and THI from dietary caramel colour for the Chinese population were considered to be of low health concern based on the present toxicological data. Soy sauce, vinegar and compound seasonings were found to be the main contributors to the dietary intake of caramel colours.


Assuntos
Carboidratos/análise , Exposição Dietética/análise , Corantes de Alimentos/análise , Imidazóis/análise , Adolescente , Adulto , Animais , Carboidratos/administração & dosagem , Criança , Pré-Escolar , Feminino , Análise de Alimentos , Corantes de Alimentos/administração & dosagem , Humanos , Imidazóis/administração & dosagem , Masculino , Pessoa de Meia-Idade , Ratos , Adulto Jovem
14.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(3): 193-198, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31030710

RESUMO

Objective To investigate the effect of Heiguteng Zhuifenghuoluo Capsule (HZC) on nuclear factor kappa B (NF-κB)-related protein in rheumatoid arthritis rats, and to analyze the expression of IκBα, NF-κBp65, phospho-nuclear factor kappa B p65 (p-NF-κBp65), receptor activator of nuclear factor κB ligand (RANKL), transforming growth factor activated kinase 1(TAK1), transforming growth factor ß activated kinase binding protein 1(TAB1), monocyte chemoattractant protein 1 (MCP-1), C-X-C motif chemokine ligand 1 (CXCL1), macrophage inflammatory protein-1 (MIP-1) of plasma in rheumatoid arthritis rats. Methods Male SD rats were randomly divided into control group, model group, HZC (0.315 g/kg) group, and HZC (0.315 g/kg) plus ammonium pyrrolidinedithiocarbamate (PDTC, 0.01 g/kg) group. The model was constructed in all groups except the control group. Five weeks after modeling, the plantar thickness and spleen organ coefficient (spleen/body weight ratio) were measured in all rats. The TAK1 and TAB1 proteins were detected by immunohistochemical method in the spleen. Western blot analysis was used to detect the expression of IκBα, NF-κBp65, p-NF-κBp65 and RANKL proteins in the synovium of joint and plantar tissue. MCP-1, CXCL1, MIP-1 expression in the plasma was detected by ELISA. Results Compared with the model group, the swelling degree of plantar and the spleen organ coefficient in the control group, HZC group and combined administration group decreased significantly. The immunohistochemistry showed that the expression of TAK1 and TAB1 proteins in the spleen were down-regulated in the control group, HZC group and combined administration group. Western blotting showed that the expression of IκBα protein was up-regulated in the synovium of joint tissue of the control group, HZC group and combined administration group, but the expression of NF-κBp65, p-NF-κBp65, RANKL proteins were down-regulated in the control group, HZC group and combined administration group. ELISA showed that the expression of plasma MCP-1, CXCL1, MIP-1 were down-regulated in the control group, HZC group and combined administration group. Conclusion HZC inhibits the inflammatory response of rheumatoid arthritis rats by regulating NF-κB-related proteins.


Assuntos
Artrite Experimental , Animais , Inflamação , Masculino , NF-kappa B , Ratos , Ratos Sprague-Dawley , Membrana Sinovial
15.
Artigo em Inglês | MEDLINE | ID: mdl-30865572

RESUMO

The aim of this study was to assess the Al dietary exposure of young Chinese children aged 0-3 years via formulae, complementary foods and wheat-based foods. Al residue data were obtained from the national food contamination monitoring programme from 2013 to 2016, encompassing 13,833 samples of 12 food items with a detection rate of 76.0%. Food consumption data were gathered from the China National Food Consumption Survey conducted in 2015, comprising 20,172 children aged 0-3 years old. The mean dietary exposure to Al for the general population of young Chinese children was estimated at 0.76 mg/kg bw/week, which does not exceed the PTWI. The 97.5th percentile intakes of Al reached 3.42 mg/kg bw/week, more than 1.7 times the PTWI. Wheat-based foods contributed 80.5% of the Al intake for the general population of young Chinese children, while formulae and complementary foods accounted for 19.5% of the total intake. The dietary intake of Al from formulae and complementary foods accounted for 6.0% and 1.6% of PTWI, respectively. These findings suggested that dietary exposure to Al among the general population of young Chinese children was lower than the PTWI and that there are no health concerns related to this level of Al intake. However, more attention should be placed on the health risks associated with Al exposure from wheat-based foods for young consumers with high food consumption in China (97.5th percentile).


Assuntos
Alumínio/análise , Exposição Dietética/análise , Análise de Alimentos , Contaminação de Alimentos/análise , Alumínio/administração & dosagem , Pré-Escolar , Humanos , Lactente , Recém-Nascido
16.
J Food Sci ; 84(3): 701-710, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30730583

RESUMO

An investigation of the naturally occurring aluminum contents in grains, fruits and vegetables locally planted in some areas of China was conducted, and the aluminum dietary intake from the investigated food was estimated. A total of 2,469 samples were collected during 2013 to 2014 and tested for aluminum content using ICP-MS method. The results showed that although 77.6% of the samples contained aluminum less than 5 mg/kg, significant variations of aluminum contents were observed in different food groups. Generally, the aluminum contents were found to be relatively high in dried grains and fresh vegetables, and low in fresh fruits. The mean value of aluminum contents in grains was 6.3 mg/kg, with wheat being the highest, followed by soybean and corn. The fresh vegetables had an average aluminum content of 4.7 mg/kg, with leafy vegetables being the highest, followed by bulb and stem vegetables. Most varieties of fresh fruits were low in aluminum, with the mean of 1.3 mg/kg. Based on the food consumption data from the China National Nutrient and Health Survey, the average weekly dietary intake of naturally occurring aluminum from the investigated foods was estimated to be 0.62 mg/kg bw for the general population and 0.55 to 1.00 mg/kg bw for different age groups. Grains and vegetables were the main contributors to the overall intake. Evaluated against the provisional tolerable weekly intake (PTWI) of 2 mg/kg bw, the dietary naturally occurring aluminum intake from the investigated foods was considered to be no safety concern.


Assuntos
Alumínio/química , Fabaceae/química , Frutas/química , Verduras/química , Adolescente , Idoso , China , Dieta , Análise de Alimentos , Humanos , Masculino , Avaliação Nutricional
17.
Artigo em Inglês | MEDLINE | ID: mdl-30650034

RESUMO

Morpholine is a common chemical used as emulsifier in the preparation of wax coatings for some fruit to help them remain fresh and protect against insects and fungal contamination. It has been reported that morpholine has acute toxic effects on rodents. In the present study, morpholine concentrations were analysed in fruits (citrus fruits, apples, strawberries and grapes) and juices (apple juice and orange juice) in order to determine dietary exposure among the Chinese population. A total of 732 fruit and juice samples were collected during 2015-2016, which covered major foods in China. Fruit and juice consumption data were taken from China National Nutrient and Health Survey (2002) and include data from 16,407 fruit or juice consumers. It was found that mean dietary exposure to morpholine residues from fruits and/or juices for general Chinese consumers and children 2-6 years old were 0.42 and 1.24 µg/kg bw/day, respectively. The 97.5% intake in general Chinese consumers and children 2-6 years old were 2.25 and 6.90 µg/kg bw/day, respectively. The primary food sources of the morpholine dietary intake of general Chinese consumers were citrus fruits (57.4%) and apples (40.8%). These findings suggested that dietary exposure to morpholine in the Chinese population was lower than the acceptable daily intake of morpholine, and there are no health concerns.


Assuntos
Dieta/estatística & dados numéricos , Exposição Dietética , Contaminação de Alimentos/análise , Sucos de Frutas e Vegetais/análise , Frutas/química , Morfolinas/análise , China
18.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 30(5): 559-562, 2018 May 22.
Artigo em Chinês | MEDLINE | ID: mdl-30567032

RESUMO

OBJECTIVE: To evaluate the effect of malaria elimination monitoring in Liyang City, so as to provide the evidence for formulating control strategies and measures of malaria elimination. METHODS: The monitoring data about the epidemic situation, blood tests of feverish patients and epidemiology investigation of individual malaria patients in Liyang City from 2010 to 2016 were collected and analyzed by the descriptive epidemiology method. RESULTS: From 2010 to 2016, there were 67 malaria cases in total. Totally 39 196 feverish patients had blood tests for Plasmodium, and 65 of them showed positive and the positive rate was 0.17%. The other 2 cases of microscopy negative were treated with anti-malarial drugs by themselves after the onset of fever, and no Plasmodium was detected in the microscopy, but the tests with malaria rapid diagnostic kit (RDTs) were positive. Among all the 67 cases, there were 49 falciparum malaria cases, 13 ovale malaria cases and 5 vivax malaria cases. All the 67 malaria cases were imported, and the number of cases from Africa was 63 (94.03%). Totally 97.01% (65/67) of the malaria patients were male and most of them were young adults. The patients aged 30 to 49 years accounted for 73.13% (49/67) and 80.60% (54/67) of them were farmers. There were malaria cases in all the 10 towns of the city, and the time of onset had no obvious seasonal characteristics. The timely rate of case report, timely rate of blood film review, standardized treatment rate, epidemiological case investigation rate, and epidemic focus investigation and disposal rate were all 100%. There were 18 076 people with the active case investigation, but no malaria parasite positive carriers were found. The mosquito vector monitoring was performed with the methods of mosquito trap lamp and human bait half night trap, and 187 and 78 Anopheles mosquitoes were captured respectively, and all the parasites were Anopheles sinensis. A total of 88 person-times were performed for the Plasmodium examinations with microscopy and RDTs (one blood sample, two detections) in Liyang City Center for Disease Prevention and Control from 2012 to 2016, and 35 person-times were positive, including 28 person-times of Plasmodium falciparum and 7 person-times of P. ovale, and there was no statistically significant difference between the detection rates of P. falciparum, and P. ovale (adjusted χ2 = 0.05, P > 0.05). There were 34 RDTs positive cases, including 14 cases of malignant malaria, and 17 cases of malignant malaria or mixed infections of P. falciparum with other three kinds of Plasmodium parasites, and 3 cases of single infection or mixed infections of other three kinds of Plasmodium parasites, and there was a statistically significant difference among them in the positive RDTs detection rates (adjusted χ2 = 13.75, P < 0.05). CONCLUSIONS: There are still imported malaria cases and there is the risk of malaria retransmission in Liyang City. Therefore, it is necessary to strengthen the malaria surveillance work and the management of infectious sources, so as to consolidate the achievements of malaria elimination in the future.


Assuntos
Erradicação de Doenças , Malária , Vigilância da População , Adulto , África , Animais , China/epidemiologia , Erradicação de Doenças/estatística & dados numéricos , Humanos , Malária/epidemiologia , Malária/prevenção & controle , Masculino , Pessoa de Meia-Idade , Adulto Jovem
19.
Cell Physiol Biochem ; 50(6): 2341-2364, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30423554

RESUMO

BACKGROUND/AIMS: Peperomin E (PepE), a natural secolignan isolated from the whole plant of Peperomia dindygulensis, has been reported by ourselves and others to display potent anti-cancer effects in many types cancer cells, especially gastric cancer. However, the effects of PepE on the metastasis of poorly-differentiated gastric cancer cells and the underlying molecular mechanisms have not been well elucidated. METHODS: We evaluated PepE effects on gastric cancer cell invasion and migration in vitro via wound healing and transwell assays and those on growth and metastasis in vivo using an orthotopic xenograft NOD-SCID mouse model. DNA methyltransferase (DNMT) activity was determined using a colorimetric DNMT activity/inhibition assay kit. PepE binding kinetics to DNMTs were determined using the bio-layer interferometry binding assay. Gene and protein levels of DNMTs, AMPKα-Sp1 signaling molecules, and metastatic-suppressor genes in PepE-treated gastric cancer cells were determined using quantitative reverse transcription-PCR arrays and western blotting. The effect of PepE on Sp1 binding to the DNMT promoter was determined by electrophoretic mobility-shift assay. Global DNA methylation levels were determined using liquid chromatography coupled with electrospray ionization tandem mass spectrometry. The methylation status of silenced metastatic-suppressor genes (MSGs) in gastric cancer cells was investigated by methylation-specific PCR. RESULTS: PepE can dose-dependently suppress invasion and migration of poorly-differentiated gastric cancer cells in vitro and in vivo with low toxicity against normal cells. Mechanistically, PepE not only covalently binds to the catalytic domain of DNMT1 and inhibits its activity (IC50 value 3.61 µM) but also down-regulates DNMT1, 3a, and 3b mRNA and protein expression in in gastric cancer cells, by disruption of the physical interaction of Sp1 with the DNMT1, 3a, and 3b promoter and mediation of the AMPKα-Sp1 signaling pathway. The dual inhibition activity of PepE toward DNMTs renders a relative global DNA hypomethylation, which induces MSG promoter hypomethylation (e.g., E-cadherin and TIMP3) and enhances their expression in gastric cancer cells. CONCLUSION: Collectively, our data indicated that PepE may represent a promising therapeutic lead compound for intervention in gastric cancer metastasis and may also exhibit potential as a DNA methylation inhibitor for use in epigenetic cancer therapy.


Assuntos
Benzodioxóis/farmacologia , DNA (Citosina-5-)-Metiltransferases/metabolismo , Metilação de DNA/efeitos dos fármacos , Proteínas Quinases Ativadas por AMP/química , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Benzodioxóis/química , Benzodioxóis/uso terapêutico , Sítios de Ligação , Movimento Celular/efeitos dos fármacos , DNA (Citosina-5-)-Metiltransferases/antagonistas & inibidores , DNA (Citosina-5-)-Metiltransferases/genética , Feminino , Humanos , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/secundário , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Simulação de Dinâmica Molecular , Regiões Promotoras Genéticas , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fator de Transcrição Sp1/química , Fator de Transcrição Sp1/metabolismo , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia , Inibidor Tecidual de Metaloproteinase-3/genética , Inibidor Tecidual de Metaloproteinase-3/metabolismo
20.
Biosci Rep ; 38(3)2018 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-29678897

RESUMO

Previous studies have demonstrated that polymorphisms in the AURKA gene are associated with various types of cancer. In neuroblastoma, AURKA protein product regulates N-myc protein levels and plays a critical role in tumorigenesis. To investigate the association between three AURKA polymorphisms (rs1047972 C>T, rs2273535 T>A, and rs8173 G>C) and neuroblastoma susceptibility in Chinese populations, we performed this two-center case-control study including 393 neuroblastoma cases and 812 controls. Two study populations were recruited from two different regions in China. No significant associations were identified amongst any of the three AURKA polymorphisms and the risk of neuroblastoma. Similar observations were found in the stratified analysis. In conclusion, our results indicate that none of the AURKA polymorphisms are associated with neuroblastoma susceptibility in two distinct Chinese populations. Further studies with larger sample sizes and different ethnicities are warranted to validate our results.


Assuntos
Aurora Quinase A/genética , Carcinogênese/genética , Predisposição Genética para Doença , Neuroblastoma/genética , Adolescente , Adulto , Feminino , Genes myc/genética , Estudos de Associação Genética , Humanos , Masculino , Pessoa de Meia-Idade , Neuroblastoma/patologia , Polimorfismo de Nucleotídeo Único , Adulto Jovem
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