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1.
Zhongguo Zhong Yao Za Zhi ; 43(16): 3270-3278, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200729

RESUMO

The system model of Chinese herbal medicine process is the basis for improving the precision of pharmaceutical process and ensuring the stable transmission of quality between different sections of the process. By taking the wet granulation-tableting process as the research object and using the Panax notoginsengs sapions (PNS) as a model drug, multi-block partial least squares method (MBPLS) was used in this article to build the system model of Chinese herbal medicine process, analyze the mass transmission rules in the process and improve the understanding on pharmaceutical process system of Chinese medicine. According to the process flow, the quality attributes of raw materials, granules and tablets collected in the process were divided into different data blocks, and the MBPLS method was used to establish system model. The R²Y and Q²Y of MBPLS model were 89.1% and 65.5%, respectively, indicating that the performance of the MBPLS model was good. By analyzing the parameters of MBPLS model, it was determined that the physical quality attributes of PNS raw materials and quality attributes of PNS granules were the key factors to affect the quality of PNS tablets. The particle size, bulk density and tapped density of PNS raw materials were the critical quality attributes which caused the quality variations of PNS granules and PNS tablets. MBPLS can quantify the contributions of each unit to the product quality by dividing the quality attributes into different units. It was helpful to analyze the interactions between the process units of Chinese herbal medicine and it could be applied to build system model of other Chinese herbal preparations processes.


Assuntos
Medicamentos de Ervas Chinesas/normas , Análise dos Mínimos Quadrados , Panax notoginseng/química , Saponinas/química , Tamanho da Partícula , Controle de Qualidade , Comprimidos
2.
J Ethnopharmacol ; 193: 36-44, 2016 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-27396350

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese medicine (TCM) has been widely used in China and its surrounding countries in clinical treatments for centuries-long time. However, due to the complexity of TCM constituents, both action mechanism and material base of TCM remain nearly unknown. AIM OF THE STUDY: The present study was designed to uncover the action mechanism and material base of TCM in a low-cost manner. MATERIALS AND METHODS: Compound Danshen dripping pills (DSP) is a widely used TCM for treatment of atherosclerosis, and was researched here to demonstrate the effectiveness of our method. We constructed a heterogeneous network for DSP, identified the significant network module, and analyzed the primary pharmacological units by performing GO and pathways enrichment analysis. RESULTS: Two significant network modules were identified from the heterogeneous network of DSP, and three compounds out of four hub nodes in the network were found to intervene in the process of atherosclerosis. Moreover, 13 out of 20 enriched pathways that were ranked in top 10 corresponding to both the two pharmacological units were found to be involved in the process of atherosclerosis. CONCLUSIONS: Quercetin, luteolin and apigenin may be the main active compounds which modulate the signaling pathways, such as metabolism of xenobiotics by cytochrome P450, retinol metabolism, etc. The present method helps reveal the action mechanism and material base of DSP for treatment of atherosclerosis.


Assuntos
Aterosclerose/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Medicina Tradicional Chinesa
3.
J Pharm Biomed Anal ; 123: 63-73, 2016 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-26874256

RESUMO

Previously published reports have revealed the antidepressant-like effects of icariin in a chronic mild stress model of depression and in a social defeat stress model in mice. However, the therapeutic effect of icariin in an animal model of glucocorticoid-induced depression remains unclear. This study aimed to investigate antidepressant-like effect and the possible mechanisms of icariin in a rat model of corticosterone (CORT)-induced depression by using a combination of behavioral and biochemical assessments and NMR-based metabonomics. The depression model was established by subcutaneous injections of CORT for 21 consecutive days in rats, as evidenced by reduced sucrose intake and hippocampal brain-derived neurotrophic factor (BDNF) levels, together with an increase in immobility time in a forced swim test (FST). Icariin significantly increased sucrose intake and hippocampal BDNF level and decreased the immobility time in FST in CORT-induced depressive rats, suggesting its potent antidepressant activity. Moreover, metabonomic analysis identified eight, five and three potential biomarkers associated with depression in serum, urine and brain tissue extract, respectively. These biomarkers are primarily involved in energy metabolism, lipid metabolism, amino acid metabolism and gut microbe metabolism. Icariin reversed the pathological process of CORT-induced depression, partially via regulation of the disturbed metabolic pathways. These results provide important mechanistic insights into the protective effects of icariin against CORT-induced depression and metabolic dysfunction.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/metabolismo , Corticosterona/farmacologia , Depressão/induzido quimicamente , Depressão/tratamento farmacológico , Flavonoides/farmacologia , Hipocampo/efeitos dos fármacos , Redes e Vias Metabólicas/efeitos dos fármacos , Animais , Antidepressivos/farmacologia , Comportamento Animal/efeitos dos fármacos , Modelos Animais de Doenças , Hipocampo/metabolismo , Imageamento por Ressonância Magnética/métodos , Masculino , Metabolômica/métodos , Ratos , Ratos Sprague-Dawley , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/metabolismo , Natação/fisiologia
4.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(7): 2098-2103, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30035893

RESUMO

To establish a new identification method of Gypsum Fibrosum with FTIR and analyze different kinds of the sulfate mineral medicine with clustering method. With the use of FTIR, identify different kinds of sulfate mineral medicine, such as gypsum fibrosum, gypsum ustum and natrii sulfas , in addition, clustering method and first derivative spectra are used to analyze the intrinsic relationship in these kinds of sulfate mineral medicine. What's more, 24 batches of Gypsum Fibrosum samples which from different production are analyzed with FTIR; then the spectrum control fingerprint with pattern is established with common peaks; at last the similarity between the simple spectrum fingerprint and the spectrum control fingerprint is calculated with angle cosine value and correlation coefficient. There are distinctions about the wave number, the peak position, the spike and the peak intensity in the infrared spectroscopy and first derivative spectra. Therefore, the sulfate mineral medicine is divided into two main regions with the based on these clusters: put the gypsum fibrosum and gypsum ustum in the same class, the natrii sulfas in the other class. Between 400 and 4 000 cm-1, the experiment showed that 24 batches of samples were more than 0.980 0. Provide a new, rapid and specificity method for identification and standard of gypsum fibrosum and build a successful cluster analysis about the sulfate mineral medicine.

5.
Zhong Yao Cai ; 39(7): 1628-32, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-30204366

RESUMO

Objective: To investigate the active components and potential mechanism of Puerariae Radix in improving insulin resistance by using network pharmacological method. Methods: Key target proteins related with insulin resistance were selected based on molecular docking technology, and then took the selected components with 31 target proteins of four pathways for docking. Meanwhile, component-target proteins network was established to network analysis by software Cytoscape 3. 2. 1. Results: 19 compounds had close interactions with four pathways such as AMPK. There were 13 compositions can verify through literature, which revealing that active ingredients and potential molecular mechanism of Puerariae Radix in improving insulin resistance, preliminarily. Conclusion: The network pharmacological method is helpful to explore the possible active components in Puerariae Radix and elucidate the mechanism.


Assuntos
Resistência à Insulina , Pueraria , Medicamentos de Ervas Chinesas , Simulação de Acoplamento Molecular , Raízes de Plantas
6.
Zhongguo Zhong Yao Za Zhi ; 41(3): 377-382, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-28868850

RESUMO

In recent years, network pharmacology has been developed rapidly, and especially, the concept of ″network target″ has brought a new era in the field of traditional Chinese medicine (TCM). The integrity and systematicness emphasized in network pharmacology comply with the characteristics of holistic view and treatment in Chinese medicine. It can provide deeper insights into the underlying mechanisms of TCM theories, including the illustration on action mechanism of Chinese medicine, selection of pharmacodynamic materials and the combination principles of various Chinese herbs, etc. Therefore, this theory is more suitable for TCM academic characteristics and practical conditions. The key problem in network pharmacology is how to efficiently and quickly identify the interactions between large amounts of drugs and target proteins. As an efficient and high throughput way, drug-target prediction technology can reduce costs, quickly predict the component targets, and provide foundation for the application of TCM network pharmacology. In view of the large amount of compounds and target databases, different prediction methods and technologies have been developed, and used to predict the drug-target interactions. Many virtual screening technologies have been successfully applied to network pharmacology. Based on different prediction principles, drug-target prediction technology can be generally divided into four types: ligand-based prediction, receptor-based prediction, machine learning and combined prediction. In this paper, we are going to review the prediction methods of drug-target interactions and give acomprehensive elaboration of their application in network pharmacology of TCM, hoping to provide beneficial references for various Chinese medicine researchers.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Bases de Dados Factuais , Interações Medicamentosas , Tratamento Farmacológico , Medicamentos de Ervas Chinesas/química , Humanos , Medicina Tradicional Chinesa , Proteínas/antagonistas & inibidores , Proteínas/genética , Proteínas/metabolismo
7.
Zhongguo Zhong Yao Za Zhi ; 41(18): 3451-3456, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-28925131

RESUMO

To elucidate the intervention effects of Jiaotai pills(JTP) on p-chlorophenylalanine (PCPA)-induced insomnia in rats and its underlying mechanism, the insomnia model was established by single intraperitoneal injection with PCPA in rats. The locomotor activity of rats was observed, and the levels of nerve growth factor(NGF) in hypothalamus, hippocampus, prefrontal cortex and serum of rats were determined by using ELISA. Moreover, a proton nuclear magnetic resonance(¹H-NMR)-based metabonomic approach was developed to profile insomnia-related metabolites in rat serum and hippocampus and analyze the intervention effects of JTP on changes in underlying biomarkers related to locomotor activity, NGF and insomnia. According to the results, JTP could significantly suppress the locomotor activity of insomnia rats, and increase the NGF levels in hypothalamus, hippocampus, prefrontal cortex and serum of rats with insomnia. The disturbed metabolic state associated with PCPA-induced insomnia in rat serum and hippocampus could be intervened by JTP. Meanwhile, six and five potential biomarkers related to insomnia in rat serum and hippocampus were reversed by administration of JTP. In conclusion, the current study demonstrated that JTP had protective effects against PCPA-induced insomnia in rats, which was probably correlated with regulation of NGF level and metabolism of amino acids, lipids and choline.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Hipocampo/efeitos dos fármacos , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Animais , Fator de Crescimento Neural/metabolismo , Ratos , Distúrbios do Início e da Manutenção do Sono/induzido quimicamente
8.
Zhong Yao Cai ; 38(4): 781-5, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26672347

RESUMO

OBJECTIVE: To apply molecular docking technology for virtual screening of active molecules of Rhei Radix et Rhizoma, and to explore the effective substances. METHODS: 21 key targets proteins related with cerebral ischemia with 52 compounds of Rhei Radix et Rhizoma based on molecular docking technology were combined to select active small molecules. Meanwhile, multi-component protein target network was established by software Cytoscape 2. 8. 1. RESULTS: It was identified that 23 of those compounds had strong interactions with no less than 10 targets by virtual screening of molecular docking. CONCLUSION: The method of virtual screening based on molecular docking can be used to find the active components of Rhei Radix et Rhizoma in treatment of cerebral ischemia. It provides the reference for research on multi-targets of Chinese medicine compound.


Assuntos
Medicamentos de Ervas Chinesas/química , Simulação de Acoplamento Molecular , Raízes de Plantas/química , Rizoma/química , Acidente Vascular Cerebral/tratamento farmacológico , Isquemia Encefálica/tratamento farmacológico , Infarto Cerebral/tratamento farmacológico , Composição de Medicamentos , Humanos , Software
9.
Zhongguo Zhong Yao Za Zhi ; 40(11): 2195-8, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26552180

RESUMO

To explore the effective ingredients and mechanism of Ligusticum wallichii in treating brain ischemia. Four brain ischemia-related target proteins were selected in the joint screening for the 45 component in L. wallichii reported in literatures based on molecular docking by reference to the corresponding drugs in the market. According to the docking results, multiple components in L. wallichii, such as phthalides, were superior to the corresponding drugs in the market, suggesting that they may be the major effective components in L. wallichii for treating brain ischemia. The method can be used to study the material base and molecular mechanism of traditional Chinese medicines.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Ligusticum/química , Simulação de Acoplamento Molecular , Fitoterapia , Medicina Tradicional Chinesa , Óxido Nítrico Sintase Tipo II/antagonistas & inibidores , Peroxidase/antagonistas & inibidores , Fator de Necrose Tumoral alfa/antagonistas & inibidores
10.
Guang Pu Xue Yu Guang Pu Fen Xi ; 35(5): 1218-21, 2015 May.
Artigo em Chinês | MEDLINE | ID: mdl-26415431

RESUMO

To establish a phenylketonuria screening model by FTIR/ATR spectroscopy, and to compare the effects of different pretreatment methods, such as baseline correction, smoothing, derivation, Fourier deconvolution, on the model quality. A consensus partial least squares regression method (cPLS) was used to build the quantitative model of phenylalanine in dried blood spots. The effects of different pretreatment methods on the model performance were investigated, using the correlation coefficient (r), root mean square error of prediction (RMSEP), mean relative error (MRE) and predictive accuracy (Acc). The nine-point smoothing coupled with the first differential was found to perform the best. Compared with the model by the original spectra, its r, RMSEP, MRE and Acc were improved from 0. 822 7, 115. 8, 0. 395 and 94. 6 to 0. 889 9, 102. 2, 0. 286 and 100, respectively. With the advantages of fast speed, easy process, no reagents consumption and environmental protection, the present method is expected to become a simple and green technology for rapidly screening the neonatal phenylketonuria in a large population.


Assuntos
Fenilcetonúrias/diagnóstico , Espectroscopia de Infravermelho com Transformada de Fourier , Humanos , Recém-Nascido , Análise dos Mínimos Quadrados , Triagem Neonatal , Análise de Regressão
11.
J Asian Nat Prod Res ; 17(8): 823-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25908248

RESUMO

Two new triterpenoids, 3ß,6ß-dihydroxy-12-oxo-13Hα-olean-28,19ß-olide (1) and 3-oxo-olean-11,13(18)-dien-28,19ß-olide (2) were isolated from the resin of Styrax tonkinensis (Pier.) Craib. The structures of both triterpenoids were determined by physicochemical and spectroscopic methods. Compound 1 is the second triterpene found with cis-fused C/D ring from the resin, which is rarely observed in oleanane-type triterpenes.


Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Ácido Oleanólico/isolamento & purificação , Resinas Vegetais/química , Styrax/química , Medicamentos de Ervas Chinesas/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Caules de Planta/química
12.
Phytomedicine ; 22(3): 333-43, 2015 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-25837270

RESUMO

Puerarin possesses a wide spectrum of biological activities including ameliorating effects on blood stasis, but the definite mechanism of this effect is still not known. In this study, a (1)H NMR-based plasma and urinary metabonomic approach was applied to comprehensively and holistically investigate the therapeutic effects of puerarin on blood stasis and its underlying mechanisms. Puerarin was injected intraperitoneally once daily for consecutive 7 days. The blood stasis rat model was established by placing the rats in ice-cold water during the time interval between two injections of adrenaline. With pattern recognition analysis, a clear separation of blood stasis model group and healthy control group was achieved and puerarin pretreatment group was located much closer to the control group than the model group, which was consistent with results of hemorheology studies. 15 and 10 potential biomarkers associated with blood stasis in plasma and urine, respectively, which were mainly involved in energy metabolism, lipid and membrane metabolisms, amino acid metabolism and gut microbiota metabolism, were identified. Puerarin could prevent blood stasis through partially regulating the disturbed metabolic pathways. This work highlights that metabonomics is a valuable tool for studying the essence of blood stasis as well as evaluating the efficacy of the corresponding drug treatment.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Isoflavonas/farmacologia , Metabolômica , Animais , Viscosidade Sanguínea , Hematócrito , Hemorreologia/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Masculino , Medicina Tradicional Chinesa , Ratos , Ratos Sprague-Dawley
13.
Zhongguo Zhong Yao Za Zhi ; 40(16): 3308-13, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26790312

RESUMO

Action mechanism and material base of compound Danshen dripping pills in treatment of carotid atherosclerosis were discussed based on gene expression profile and molecular fingerprint in this paper. First, gene expression profiles of atherosclerotic carotid artery tissues and histologically normal tissues in human body were collected, and were screened using significance analysis of microarray (SAM) to screen out differential gene expressions; then differential genes were analyzed by Gene Ontology (GO) analysis and KEGG pathway analysis; to avoid some genes with non-outstanding differential expression but biologically importance, Gene Set Enrichment Analysis (GSEA) were performed, and 7 chemical ingredients with higher negative enrichment score were obtained by Cmap method, implying that they could reversely regulate the gene expression profiles of pathological tissues; and last, based on the hypotheses that similar structures have similar activities, 336 ingredients of compound Danshen dripping pills were compared with 7 drug molecules in 2D molecular fingerprints method. The results showed that 147 differential genes including 60 up-regulated genes and 87 down regulated genes were screened out by SAM. And in GO analysis, Biological Process ( BP) is mainly concerned with biological adhesion, response to wounding and inflammatory response; Cellular Component (CC) is mainly concerned with extracellular region, extracellular space and plasma membrane; while Molecular Function (MF) is mainly concerned with antigen binding, metalloendopeptidase activity and peptide binding. KEGG pathway analysis is mainly concerned with JAK-STAT, RIG-I like receptor and PPAR signaling pathway. There were 10 compounds, such as hexadecane, with Tanimoto coefficients greater than 0.85, which implied that they may be the active ingredients (AIs) of compound Danshen dripping pills in treatment of carotid atherosclerosis (CAs). The present method can be applied to the research on material base and molecular action mechanism of TCM.


Assuntos
Doenças das Artérias Carótidas/tratamento farmacológico , Doenças das Artérias Carótidas/genética , Medicamentos de Ervas Chinesas/administração & dosagem , Salvia miltiorrhiza/química , Doenças das Artérias Carótidas/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Transdução de Sinais/efeitos dos fármacos
14.
Zhongguo Zhong Yao Za Zhi ; 38(16): 2690-5, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24228588

RESUMO

A simple and reliable method of high-performance liquid chromatography with photodiode array detection (HPLC-DAD) was developed to evaluate the quality of a traditional Chinese medicine Sophora flavescens through establishing chromatographic fingerprint and simultaneous determination of five flavonoids, including trifolirhizin, maackiain, kushenol I, kurarinone and sophoraflavanone G. The optimal conditions of separation and detection were achieved on an ULTIMATE XB-C18 column (4.6 mm x 250 mm, 5 microm) with a gradient of acetonitrile and water, detected at 295 nm. In the chromatographic fingerprint, 13 peaks were selected as the characteristic peaks to assess the similarities of different samples collected from different origins in China according to similarity evaluation for chromatographic fingerprint of traditional chinese medicine (2004AB) and principal component analysis (PCA) were used in data analysis. There were significant differences in the fingerprint chromatograms between S. flavescens and S. tonkinensis. Principal component analysis showed that kurarinone and sophoraflavanone G were the most important component. In quantitative analysis, the five components showed good regression (R > 0.999) with linear ranges, and their recoveries were in the range of 96.3% - 102.3%. This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for S. flavescens and its related traditional Chinese medicinal preparations.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Sophora/química , Flavonoides/isolamento & purificação , Controle de Qualidade
15.
Zhongguo Zhong Yao Za Zhi ; 38(9): 1406-10, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-23944078

RESUMO

OBJECTIVE: To establish a new method for quality evaluation and validate its feasibilities by simultaneous quantitative assay of five alkaloids in Sophora flavescens. METHOD: The new quality evaluation method, quantitative analysis of multi-components by single marker (QAMS), was established and validated with S. flavescens. Five main alkaloids, oxymatrine, sophocarpine, matrine, oxysophocarpine and sophoridine, were selected as analytes to evaluate the quality of rhizome of S. flavescens, and the relative correction factor has good repeatibility. Their contents in 21 batches of samples, collected from different areas, were determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. RESULT: No significant differences were found in the quantitative results of five alkaloids in 21 batches of S. flavescens determined by external standard method and QAMS. CONCLUSION: It is feasible and suitable to evaluate the quality of rhizome of S. flavescens by QAMS.


Assuntos
Alcaloides/análise , Sophora/química , Quinolizinas/análise
16.
Yao Xue Xue Bao ; 48(11): 1733-7, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24475714

RESUMO

To investigate the intervention effects of Morinda officinalis How. on 'Kidney-yang deficiency syndrome' induced by hydrocortisone in rats, the metabolic profiles of rat urine were characterized using proton nuclear magnetic resonance and principal component analysis (PCA) was applied to study the trajectory of urinary metabolic phenotype of rats with 'Kidney-yang deficiency syndrome' under administration of M. officinalis at different time points. Meanwhile, the intervention effects of M. officinalis on urinary metabolic potential biomarkers associated with 'Kidney-yang deficiency syndrome' were also discussed. The experimental results showed that in accordance to the increased time of administration, an obvious tendency was observed that clustering of the treatment group moved gradually closed to that of the control group. Eight potential biomarkers including citrate, succinate, alpha-ketoglutarate, lactate, betaine, sarcosine, alanine and taurine were definitely up- or down-regulated. In conclusion, the effectiveness of M. oficinalis on 'Kidney-yang deficiency syndrome' is proved using the established metabonomic method and the regulated metabolic pathways involve energy metabolism, transmethylation and transportation of amine. Meanwhile, the administration of M. officinalis can alleviate the kidney impairment induced by 'Kidney-yang deficiency syndrome'.


Assuntos
Biomarcadores/urina , Medicamentos de Ervas Chinesas/farmacologia , Nefropatias/urina , Morinda/química , Deficiência da Energia Yang/urina , Alanina/urina , Animais , Betaína/urina , Ácido Cítrico/urina , Medicamentos de Ervas Chinesas/isolamento & purificação , Hidrocortisona , Ácidos Cetoglutáricos/urina , Nefropatias/induzido quimicamente , Ácido Láctico/urina , Espectroscopia de Ressonância Magnética , Masculino , Metabolômica/métodos , Plantas Medicinais/química , Análise de Componente Principal , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Sarcosina/urina , Ácido Succínico/urina , Taurina/urina , Deficiência da Energia Yang/induzido quimicamente
17.
J Ethnopharmacol ; 144(1): 208-12, 2012 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-22982395

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Naodesheng prescription is a widely used traditional Chinese medicine (TCM), and mainly applied in clinics to treat cerebral arteriosclerosis, ischemic stroke, sequelae of cerebral hemorrhage etc. AIM OF THE STUDY: The present study was designed to investigate the anti-thrombosis effects of this prescription, and to search its bioactive fraction. MATERIALS AND METHODS: We evaluated the anti-thrombotic effects by measuring platelet aggregation in rats, and the clotting time and protection rate in mice. The contents of total saponins and flavonoids in the bioactive fraction were determined by UV-vis spectrophotometry. RESULTS: The results showed that Naodesheng has significant anti-thrombotic and anticoagulation effects. The contents of total saponins and flavonoids in the bioactive fraction were 7.885% and 8.434%, respectively. CONCLUSIONS: Such study will contribute to further exploration of Naodesheng in the therapy of thrombosis diseases and to its re-development based on the bioactive fraction and ingredients.


Assuntos
Anticoagulantes/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Fibrinolíticos/farmacologia , Inibidores da Agregação Plaquetária/farmacologia , Animais , Coagulação Sanguínea/efeitos dos fármacos , Medicamentos de Ervas Chinesas/análise , Feminino , Flavonoides/análise , Masculino , Camundongos , Agregação Plaquetária/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Saponinas/análise , Trombose/prevenção & controle
18.
Yao Xue Xue Bao ; 47(4): 434-9, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22799023

RESUMO

Zebrafish has become an important model organism in many fields of biomedical studies and been increasingly used in Chinese materia medica studies in recent years. This article summarized the achievements and prospect for zebrafish as a pharmacological and toxicological tool in the study and development of Chinese materia medica.


Assuntos
Indutores da Angiogênese/farmacologia , Inibidores da Angiogênese/farmacologia , Modelos Animais de Doenças , Materia Medica , Medicina Tradicional Chinesa , Animais , Materia Medica/farmacologia , Materia Medica/uso terapêutico , Materia Medica/toxicidade , Transtornos da Memória/prevenção & controle , Neovascularização Fisiológica/efeitos dos fármacos , Peixe-Zebra
19.
Anal Chim Acta ; 713: 30-5, 2012 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-22200304

RESUMO

Herbal medicine has been successfully applied in clinical therapeutics throughout the world. In this paper, various information contents in addition to a best-first search strategy are proposed for improving prediction quality of quantitative profile-efficacy relationship (QPER). As a demonstration, a QPER model was constructed to predict the blood-clotting time increasing effect of Naodesheng prescription, a widely used herbal medicine in China. Evaluated by both the self-consistency test and the rigorous jackknife test, the predictive accuracy was increased by feeding the model with the merged information contents compared to only with the common chromatographic peak areas. The confirmation experiment exhibited that the predicted blood-clotting time were well consistent with the observed values. Furthermore, it has not escaped from our notice that the important potential application of the current QPER model is to be extended to help search active fractions, which has attracted more and more attention in the re-developments of herbal medicines based on the active compounds.


Assuntos
Modelos Teóricos , Relação Quantitativa Estrutura-Atividade , Animais , Testes de Coagulação Sanguínea , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Masculino , Camundongos
20.
Zhong Yao Cai ; 34(7): 1078-80, 2011 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22066403

RESUMO

OBJECTIVE: To screen the antithrombotic active fraction of Naodesheng. METHODS: The collagen and adrenaline-induced thrombosis by tail intravenous injection in mice was used. Based on orthogonal design, the support vector machine model was established for pharmacodynamics prediction of TCM prescriptions. RESULTS: The model established in this study could predict the drug actions of different combinations. CONCLUSION: The pharmacodynamic actions of several formulas are superior to that of the original formula and CD has the best effect.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Fibrinolíticos/química , Fibrinolíticos/farmacologia , Máquina de Vetores de Suporte , Trombose/tratamento farmacológico , Algoritmos , Animais , Fracionamento Químico , Simulação por Computador , Combinação de Medicamentos , Descoberta de Drogas/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Feminino , Fibrinolíticos/isolamento & purificação , Masculino , Camundongos , Camundongos Endogâmicos , Modelos Estatísticos , Plantas Medicinais/química , Distribuição Aleatória , Relação Estrutura-Atividade , Trombose/induzido quimicamente
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