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Biosci Biotechnol Biochem ; 83(10): 1955-1963, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31200628


A metabolomics method was established to analyze changes of intracellular metabolites and study the mechanism for enhancing polyhydroxyalkanoates production by halotolerant bacteria, Bacillus cereus strain HY-3, using acetic acid as carbon source. Maximum poly(3-hydroxybutyrate) (PHB) contents for the medium with 0.5 g/L and 5.0 g/L of acetic acid were 41.0 ± 0.415% and 49.2 ± 1.21%. Principal components analysis revealed clear metabolic differences in different growth stages and different concentrations of carbon source. According to statistical analysis, 3-hydroxybutyrate (3-HB), serine, threonine, malate, and pyruvate were determined as potential biomarkers for PHB production. Moreover, metabolic pathways analysis indicated that high level of 3-HB in death phase was due to the limitation of carbon source. Metabolism of glycine, serine, and threonine was influential pathway for PHB production among amino acid metabolisms. High levels of organic acids from the TCA cycle could stimulate the carbon source flux into PHB biosynthetic pathway.

Sichuan Da Xue Xue Bao Yi Xue Ban ; 49(3): 380-383, 2018 May.
Artigo em Chinês | MEDLINE | ID: mdl-30014638


OBJECTIVE: To explore the effects of calcium pump inhibitor thapsigargin (TG) on intracellular Ca2+ and the expression of caspase-3 protein in mouse lung fibroblast. METHODS: Mice lung fibroblast cells were divided into three groups: the blank control group,transforming growth factor-ß1 (TGF-ß1) group and TG treated group. The cells were induced by 5 ng/mL TGF-ß1 for 24 h in TGF-ß1 group and TG group,4 µmol/L TG was added in TG group for 24 h. After 48 h,the cells were collected,and the cell structure was observed by transmission electron microscope,intracellular Ca2+ level was detected with laser confocal microscope,the protein expression of caspase-3 was examined using immunohistochemistry method. RESULTS: Transmission electron microscopy showed that the cells in the blank control group had obvious nucleolus,complete organelles and less apoptosis. In TGF-ß1 group,the cell morphology was intact,chromatin was evenly distributed,and no apoptotic cells were found. In TG group,there were a large number of apoptosis of fibroblasts,chromatin clumps in nuclei and a small amount of collagen fibers. The level of Ca2+ in TGF-ß1 group was significantly lower than that in control group (P<0.05),and which in TG group was significantly higher (P<0.05 ). The protein expression of caspase-3 in TGF-ß1 group were significantly lower than that in blank control group (P<0.05),which in TG group increased obviously (P<0.05). CONCLUSION: TG could cause intracellular calcium dysregulation in mouse lung fibroblasts,increase caspase-3 protein expression and promote cell apoptosis.

Anal Chem ; 88(18): 8997-9003, 2016 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-27558867


Highly effective targeted tumor recognition via vectors is crucial for cancer detection. In contrast to antibodies and proteins, peptides are direct targeting ligands with a low molecular weight. In the present study, a peptide magnetic nanovector platform containing a lipid bilayer was designed using a peptide amphiphile (PA) as a skeleton material in a controlled manner without surface modification. Fluorescein isothiocyanate-labeled epidermal growth factor receptor (EGFR) peptide nanoparticles (NPs) could specifically bind to EGFR-positive liver tumor cells. EGFR peptide magnetic vesicles (EPMVs) could efficiently recognize and separate hepatoma carcinoma cells from cell solutions and treated blood samples (ratio of magnetic EPMVs versus anti-EpCAM NPs: 3.5 ± 0.29). Analysis of the circulating tumor cell (CTC) count in blood samples from 32 patients with liver cancer showed that EPMVs could be effectively applied for CTC capture. Thus, this nanoscale, targeted cargo-packaging technology may be useful for designing cancer diagnostic systems.

Separação Celular/métodos , Receptores ErbB/metabolismo , Neoplasias Hepáticas/patologia , Células Neoplásicas Circulantes/patologia , Peptídeos/metabolismo , Linhagem Celular Tumoral , Humanos , Bicamadas Lipídicas/metabolismo , Neoplasias Hepáticas/sangue , Imãs/química , Nanocápsulas/química , Células Neoplásicas Circulantes/metabolismo
Sichuan Da Xue Xue Bao Yi Xue Ban ; 46(1): 62-4, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-25807798


OBJECTIVE: To observe the effects of different concentration of 17P-estradiol (17p-E2) on the expression of Caveolin-1 and type 11 collagen in the mouse lung fibroblast stimulated by SiO2. METHODS: Fibroblast cells were devided into five groups: blank control group, Si02 (100 mg/L) group and SiOz (100 mg/L)+ different concentration of 17beta-E2 (10(-8),10(-6),10(-6) mol/L) groups. After treated with different concentration of 17beta-E2, for 48 h, the cells were collected, then the expression of Caveolin-1 and of type III collagen were examined with immunohistochemistry method. RESULTS: Compared with the blank control group, the expression of Caveolin-1 in mouse lung fibroblasts treated by SiO2 significantly decreased (P<0.05), While which in the SiO2 +17P-E2 group significantly increased as 17beta-E2 dose increased (P<0.05); There were significantly different in the expression of type III collagen among different groups (P<0.05), which in SiO2 group were evidently higher than that in the blank control group, while which in 17p-E2 groups decreased significantly, when compared with SiO2 group (P< 0.05); 17beta-E2 increased the expression of Caveolin-1 and decreased the expression of type 1f collagen in the dose dependent manner. Correlation analysis showed that 17p-E2 was positive correlated with the expression of Caveolin-1 (r=0.926, P<0.05), and negative correlated with the express of type Ill collagen (r = -0.914, P<0.05), and the expression of Caveolin-1 and the expression of type III collagen was negatively correlation (r = -0.887, P<0.05). CONCLUSION: 17beta-E2 may inhibit the expression of type III collagen by up-regulating the expression of Caveolin-1 in mouse lung fibroblast cell to play a role in the resistance of the lung fibrosis.

Caveolina 1/metabolismo , Colágeno Tipo III/metabolismo , Estradiol/farmacologia , Fibroblastos/efeitos dos fármacos , Animais , Células Cultivadas , Fibroblastos/metabolismo , Pulmão/citologia , Camundongos , Fibrose Pulmonar , Dióxido de Silício/farmacologia , Regulação para Cima
Zhonghua Yi Xue Za Zhi ; 91(3): 193-7, 2011 Jan 18.
Artigo em Chinês | MEDLINE | ID: mdl-21418902


OBJECTIVE: To evaluate the ability of a kind of novel magnetic liposomes modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) to cross the blood spinal cord barrier (BSCB) so as to demonstrate whether or not they can accumulate at the lesions of injured spinal cord. METHODS: The novel liposomes were made through reverse-phase evaporation method modified with polyethylene glycol (PEG) and transactivating-transduction protein (TAT) with an iron core. Thirty-six Wistar rats subject to spinal cord injury (SCI) at T10 were randomly divided into three groups (Groups I, II and III). The rats of Group III were injected with TAT-PEG loaded magnetic liposomes (4.55 mg/kg). The rats of GroupII received an injection of the equivalent PEG loaded magnetic liposomes while those of control group (GroupI) the equivalent normal saline. The accumulation of liposomes was observed by MRI (magnetic resonance imaging), Prussian blue staining, electron microscope and flame atomic absorption spectrophotometer. RESULTS: This kind of TAT-PEG loaded magnetic liposomes could cross the BSCB and enter into the cells around the injured tissue. A low signal of T2WI on MRI could also be found in Group III. The results of flame atomic absorption spectrophotometer showed that the iron content accumulated around the lesion site in Group III was obviously higher than the other two groups (P < 0.05). CONCLUSION: The TAT-PEG loaded magnetic liposomes may be employed as one kind of novel drug carrier to cross the BSCB and accumulate at tissue cells of spinal cord. It is likely to become a new therapy for SCI.

Portadores de Fármacos , Traumatismos da Medula Espinal/sangue , Medula Espinal/irrigação sanguínea , Animais , Modelos Animais de Doenças , Produtos do Gene tat/administração & dosagem , Produtos do Gene tat/farmacocinética , Lipossomos , Magnetismo , Masculino , Nanoestruturas , Polietilenoglicóis/administração & dosagem , Polietilenoglicóis/farmacocinética , Ratos , Ratos Wistar , Traumatismos da Medula Espinal/metabolismo