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1.
Neuropsychiatr Dis Treat ; 18: 943-950, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35535212

RESUMO

Purpose: Stroke-associated infection (SAI) is one of the most common post-stroke complications, which may lead to a relatively poor prognosis. This study aims to explore the potential relationship between serum calcium levels and SAI. Patients and Methods: This is a cross-sectional study involving 395 participants. SAI is a term that covers stroke-associated pneumonia (SAP), urinary tract infection (UTI), as well as other infections diagnosed during the first week after a stroke. Serum calcium levels were measured within 24 hrs of admission. To determine the association of serum calcium levels with the SAI, logistic regression models were calculated. An adjusted spline regression model was used to further confirm the relationship between the two. Results: Among the total patients, SAI occurred in 107 (27.1%) patients. Serum calcium was associated with SAI and was independent of the SAI risk factors for age, admission NIHSS score, and intravascular therapy [adjusted OR 0.040 (95% CI = 0.005-0.291), p = 0.002]. Remarkably, this trend applies predominantly to serum calcium levels less than 2.25mmol/L (p= 0.005 for linearity). Conclusion: Serum calcium levels are associated with the risk of SAI, when serum calcium is below normal (2.25mmol/L), the risk of SAI increases as serum calcium levels decrease.

3.
Microbiol Spectr ; 10(2): e0206421, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35230154

RESUMO

Two tet(X4)-positive Enterobacter cloacae isolates TECL_1 and TECL_2 were isolated from pigs in China. S1-PFGE and Southern blotting showed that tet(X4) located on plasmids in the size of ∼290 kb and ∼190 kb in TECL_1 and TECL_2, respectively. Conjugation experiment demonstrated that the tet(X4)-harboring plasmid can transfer from the donor strain TECL_1 and TECL_2 to the recipient strain Escherichia coli J53, and the tigecycline resistance of transconjugants was increased by 128-fold and 64-fold compared with E. coli J53, respectively. We obtained the complete plasmid sequence of pTECL_2-190k-tetX4 (190,185 bp) from E. cloacae TECL_2 and found that the plasmid was a hybrid plasmid with replicon types of IncFIA, IncHI1A and IncHI1B. We further analyzed 85 tet(X4)-carrying plasmids in the public database and clarified that pTECL_2-190k-tetX4-like plasmid was widespread in multiple species of Enterobacteriaceae. IMPORTANCE We identified two tet(X4)-positive E. cloacae isolates, which has not been previously reported. We obtained the complete sequence of pTECL_2-190k-tetX4 and found that it was a hybrid plasmid with multiple replicon types, including IncFIA, IncHI1A and IncHI1B. By comparing all the known tet(X4)-carrying plasmids, we found that pTECL_2-190k-tetX4-like plasmid has been disseminated across various species in China. Our study expanded the identification of tet(X4)-positive species and emphasized that pTECL_2-190k-tetX4-like plasmid has spread widely in various species.


Assuntos
Enterobacter cloacae , Escherichia coli , Animais , Antibacterianos/farmacologia , China , Enterobacter cloacae/genética , Escherichia coli/genética , Testes de Sensibilidade Microbiana , Plasmídeos/genética , Suínos , Tigeciclina/farmacologia
4.
BMC Genomics ; 23(1): 20, 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-34996351

RESUMO

BACKGROUND: Carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) causes serious infections with significant morbidity and mortality. However, the epidemiology and transmission mechanisms of CR-hvKP and the corresponding carbapenem-resistant plasmids require further investigation. Herein, we have characterized an ST11 K. pneumoniae strain EBSI041 from the blood sample encoding both hypervirulence and carbapenem resistance phenotypes from a patient in Egypt. RESULTS: K. pneumoniae strain EBSI041 showed multidrug-resistance phenotypes, where it was highly resistant to almost all tested antibiotics including carbapenems. And hypervirulence phenotypes of EBSI041 was confirmed by the model of Galleria mellonella infection. Whole-genome sequencing analysis showed that the hybrid plasmid pEBSI041-1 carried a set of virulence factors rmpA, rmpA2, iucABCD and iutA, and six resistance genes aph(3')-VI, armA, msr(E), mph(E), qnrS, and sul2. Besides, blaOXA-48 and blaSHV-12 were harboured in a novel conjugative IncL-type plasmid pEBSI041-2. The blaKPC-2-carrying plasmid pEBSI041-3, a non-conjugative plasmid lacking the conjugative transfer genes, could be transferred with the help of pEBSI041-2, and the two plasmids could fuse into a new plasmid during co-transfer. Moreover, the emergence of the p16HN-263_KPC-like plasmids is likely due to the integration of pEBSI041-3 and pEBSI041-4 via IS26-mediated rearrangement. CONCLUSION: To the best of our knowledge, this is the first report on the complete genome sequence of KPC-2- and OXA-48-coproducing hypervirulent K. pneumoniae from Egypt. These results give new insights into the adaptation and evolution of K. pneumoniae during nosocomial infections.


Assuntos
Infecções por Klebsiella , Klebsiella pneumoniae , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Carbapenêmicos/farmacologia , Egito , Humanos , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/genética , Plasmídeos/genética , beta-Lactamases/genética
5.
Environ Int ; 158: 107007, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34991266

RESUMO

Driven by increasingly stringent regulations on the legacy poly/perfluoroalkyl (PFASs), a variety of fluorinated alternatives have emerged on the market. Tibetan Plateau (TP) plays an important role in accumulation of organic pollutants due to its high altitude and wet deposition. In this study, the occurrence, spatial distribution and sources of PFASs in the TP soils were investigated. The total concentrations of PFASs ranged from 0.814-4.51 ng/g in the TP soils, with the identification of a variety of novel PFASs, including fluorotelomer sulfonates (FTSs), chlorinated polyfluorinated ether sulfonic acid (Cl-PFESAs), and hexafluoropropylene oxide (HFPO) homologues. Generally, the PFAS concentrations exhibited an increase trend from the west to east, and gradually increased with the altitude increasing, suggesting the impacts of human activities and mountain cold-trapping. The PFASs decreased with the increase of soil depth, but at different extents, which were related to their occurrence time, interactions with organic matters, and microbial transformation in soil. Most of the PFASs were present as free fractions in soil, particularly for the short-chain perfluoroalkyl acids (PFAAs), implying that they were liable to be accumulated in organisms and transport to groundwater. Multiple source apportionment analyses indicated that PFASs in the soil of TP were not only derived from the local pollution, but also from the atmospheric migration influenced by Indian Monsoon and westerly winds.


Assuntos
Fluorcarbonetos , Poluentes Químicos da Água , Monitoramento Ambiental , Fluorcarbonetos/análise , Humanos , Solo , Tibet , Poluentes Químicos da Água/análise
6.
Front Cell Infect Microbiol ; 11: 684965, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34737971

RESUMO

Background: The diagnosis of bacterial pathogens in lower respiratory tract infections (LRI) using conventional culture methods remains challenging and time-consuming. Objectives: To evaluate the clinical performance of a rapid nanopore-sequencing based metagenomics test for diagnosis of bacterial pathogens in common LRIs through a large-scale prospective study. Methods: We enrolled 292 hospitalized patients suspected to have LRIs between November 2018 and June 2019 in a single-center, prospective cohort study. Rapid clinical metagenomics test was performed on-site, and the results were compared with those of routine microbiology tests. Results: 171 bronchoalveolar lavage fluid (BAL) and 121 sputum samples were collected from patients with six kinds of LRIs. The turnaround time (from sample registration to result) for the rapid metagenomics test was 6.4 ± 1.4 hours, compared to 94.8 ± 34.9 hours for routine culture. Compared with culture and real-time PCR validation tests, rapid metagenomics achieved 96.6% sensitivity and 88.0% specificity and identified pathogens in 63 out of 161 (39.1%) culture-negative samples. Correlation between enriched anaerobes and lung abscess was observed by Gene Set Enrichment Analysis. Moreover, 38 anaerobic species failed in culture was identified by metagenomics sequencing. The hypothetical impact of metagenomics test proposed antibiotic de-escalation in 34 patients compared to 1 using routine culture. Conclusions: Rapid clinical metagenomics test improved pathogen detection yield in the diagnosis of LRI. Empirical antimicrobial therapy could be de-escalated if rapid metagenomics test results were hypothetically applied to clinical management.


Assuntos
Nanoporos , Pneumonia Bacteriana , Bactérias/genética , Humanos , Metagenômica , Estudos Prospectivos , Sensibilidade e Especificidade
7.
FEBS Open Bio ; 11(7): 1953-1964, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33993653

RESUMO

Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) plays a crucial role in maintaining vascular homeostasis. As a hallmark of eNOS activation, phosphorylation of eNOS at Ser1177 induced by activated protein kinase B (PKB/Akt) is pivotal for NO production. The complete activation of Akt requires its phosphorylation of both Thr308 and Ser473. However, which site plays the main role in regulating phosphorylation of eNOS Ser1177 is still controversial. The purpose of the present study is to explore the specific regulatory mechanism of phosphorylated Akt in eNOS activation. Inhibition of Akt Thr308 phosphorylation by a specific inhibitor or by siRNA in vitro led to a decrease in eNOS phosphorylation at Ser1177 and to lower NO concentration in the cell culture medium of HUVECs. However, inhibiting p-Akt Ser473 had no effect on eNOS phosphorylation at Ser1177. Next, we administered mice with inhibitors to downregulate p-Akt Ser473 or Thr308 activity. Along with the inhibition of p-Akt Thr308, vascular p-eNOS Ser1177 protein was simultaneously downregulated in parallel with a decrease in plasma NO concentration. Additionally, we cultured HUVECs at various temperature conditions (37, 22, and 4 °C). The results showed that p-Akt Ser473 was gradually decreased in line with the reduction in temperature, accompanied by increased levels of p-Akt Thr308 and p-eNOS Ser1177. Taken together, our study indicates that the phosphorylation of Akt at Thr308, but not at Ser473, plays a more significant role in regulating p-eNOS Ser1177 levels under physiological conditions.


Assuntos
Óxido Nítrico Sintase Tipo III , Proteínas Proto-Oncogênicas c-akt , Animais , Camundongos , Óxido Nítrico/metabolismo , Óxido Nítrico/farmacologia , Óxido Nítrico Sintase Tipo III/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
8.
mSphere ; 6(3)2021 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-34011682

RESUMO

The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates in Egyptian hospitals has been reported. However, the genetic basis and analysis of the plasmids associated with carbapenem-resistant hypervirulent K. pneumoniae (CR-HvKP) in Egypt have not been presented. Therefore, we attempted to decipher the plasmid sequences that are responsible for transferring the determinants of carbapenem resistance, particularly bla NDM-1 and bla KPC-2 Out of 34 K. pneumoniae isolates collected from two tertiary hospitals in Egypt, 31 were CRKP. Whole-genome sequencing revealed that our isolates were related to 13 different sequence types (STs). The most prevalent ST was ST101, followed by ST383 and ST11. Among the CRKP isolates, one isolate named EBSI036 has been reassessed by Nanopore sequencing. Genetic environment analysis showed that EBSI036 carried 20 antibiotic resistance genes and was identified as a CR-HvKP strain: it harbored four plasmids, namely, pEBSI036-1-NDM-VIR, pEBSI036-2-KPC, pEBSI036-3, and pEBSI036-4. The two carbapenemase genes bla NDM-1 and bla KPC-2 were located on plasmids pEBSI036-1-NDM-VIR and pEBSI036-2-KPC, respectively. The IncFIB:IncHI1B hybrid plasmid pEBSI036-1-NDM-VIR also carried some virulence factors, including the regulator of the mucoid phenotype (rmpA), the regulator of mucoid phenotype 2 (rmpA2), and aerobactin (iucABCD and iutA). Thus, we set out in this study to analyze in depth the genetic basis of the pEBSI036-1-NDM-VIR and pEBSI036-2-KPC plasmids. We report a high-risk clone ST11 KL47 serotype of a CR-HvKP strain isolated from the blood of a 60-year-old hospitalized female patient from the intensive care unit (ICU) in a tertiary care hospital in Egypt, which showed the cohabitation of a novel hybrid plasmid coharboring the bla NDM-1 and virulence genes and a bla KPC-2-carrying plasmid.IMPORTANCE CRKP has been registered in the critical priority tier by the World Health Organization and has become a significant menace to public health. The emergence of CR-HvKP is of great concern in terms of both disease and treatment. In-depth analysis of the carbapenemase-encoding and virulence plasmids may provide insight into ongoing recombination and evolution of virulence and multidrug resistance in K. pneumoniae Thus, this study serves to alert contagious disease clinicians to the presence of hypervirulence in CRKP isolates in Egyptian hospitals.


Assuntos
Antibacterianos/farmacologia , Carbapenêmicos/farmacologia , Klebsiella pneumoniae/enzimologia , Klebsiella pneumoniae/patogenicidade , Plasmídeos/genética , Fatores de Virulência/genética , beta-Lactamases/genética , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Egito , Feminino , Humanos , Lactente , Recém-Nascido , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Centros de Atenção Terciária/estatística & dados numéricos , Adulto Jovem
9.
Emerg Microbes Infect ; 10(1): 700-709, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33739229

RESUMO

Bloodstream infections (BSIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are potentially life-threatening and an urgent threat to public health. The present study aims to clarify the characteristics of carbapenemase-encoding and virulent plasmids, and their interactions with the host bacterium. A total of 425 Kp isolates were collected from the blood of BSI patients from nine Chinese hospitals, between 2005 and 2019. Integrated epidemiological and genomic data showed that ST11 and ST307 Kp isolates were associated with nosocomial outbreak and transmission. Comparative analysis of 147 Kp genomes and 39 completely assembled chromosomes revealed extensive interruption of acrR by ISKpn26 in all Kp carbapenemase-2 (KPC-2)-producing ST11 Kp isolates, leading to activation of the AcrAB-Tolc multidrug efflux pump and a subsequent reduction in susceptibility to the last-resort antibiotic tigecycline and six other antibiotics. We described 29 KPC-2 plasmids showing diverse structures, two virulence plasmids in two KPC-2-producing Kp, and two novel multidrug-resistant (MDR)-virulent plasmids. This study revealed a multifactorial impact of KPC-2 plasmid on Kp, which may be associated with nosocomial dissemination of MDR isolates.


Assuntos
Antibacterianos/farmacologia , Carbapenêmicos/farmacologia , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/isolamento & purificação , Sepse/microbiologia , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , China/epidemiologia , Farmacorresistência Bacteriana , Humanos , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/classificação , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/patogenicidade , Testes de Sensibilidade Microbiana , Epidemiologia Molecular , Mariposas , Filogenia , Sepse/epidemiologia , Virulência , beta-Lactamases/genética , beta-Lactamases/metabolismo
10.
Int Immunopharmacol ; 93: 107388, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33529913

RESUMO

Endothelial dysfunction is a typical characteristic of sepsis. Endothelial nitric oxide synthase (eNOS) is important for maintaining endothelial function. Our previous study reported that the NLRP3 inflammasome promoted endothelial dysfunction by enhancing inflammation. However, the effects of NLRP3 on eNOS require further investigation. Therefore, the present study aimed to investigate the role of NLRP3 on eNOS expression levels in cecal ligation and puncture-induced impaired endothelium-dependent vascular relaxation and to determine the protective effects of melatonin. eNOS expression levels were discovered to be downregulated in the mesenteric arteries of sepsis model mice. Inhibiting NLRP3 with 10 mg/ kg MCC950 or inhibiting IL-1ß with 100 mg diacerein rescued the eNOS expression and improved endothelium-dependent vascular relaxation. In vitro, IL-1ß stimulation downregulated eNOS expression levels in human aortic endothelial cells (HAECs) in a concentration- and time-dependent manner, while pretreatment with 1 µM of the proteasome inhibitor MG132 reversed this effect. In addition, treatment with 10 mg/kg MG132 also prevented the proteolysis of eNOS and improved endothelium-dependent vascular relaxation in vivo. Notably, treatment with 30 mg/kg melatonin downregulated NLRP3 expression levels and decreased IL-1ß secretion, subsequently increasing the expression of eNOS and improving endothelium-dependent vascular relaxation. In conclusion, the findings of the present study indicated that the NLRP3/IL-1ß axis may impair vasodilation by promoting the proteolysis of eNOS and melatonin may protect against sepsis-induced endothelial relaxation dysfunction by inhibiting the NLRP3/IL-1ß axis, suggesting its pharmacological potential in sepsis.


Assuntos
Interleucina-1beta/fisiologia , Melatonina/uso terapêutico , Proteína 3 que Contém Domínio de Pirina da Família NLR/fisiologia , Óxido Nítrico Sintase Tipo III/metabolismo , Sepse/tratamento farmacológico , Vasodilatação/fisiologia , Animais , Aorta/citologia , Células Cultivadas , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/fisiologia , Humanos , Masculino , Melatonina/farmacologia , Artérias Mesentéricas/efeitos dos fármacos , Artérias Mesentéricas/fisiologia , Camundongos Endogâmicos C57BL , Proteólise , Sepse/metabolismo , Sepse/fisiopatologia , Vasodilatação/efeitos dos fármacos
11.
J Hazard Mater ; 404(Pt A): 124134, 2021 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-33022527

RESUMO

In this study, legacy and novel per and polyfluoroalkyl substances (PFASs) were measured in groundwater samples collected from the Loess Plateau of China to understand their occurrence, sources and health risks. The total concentration of PFASs ranged from 2.78 to 115 ng/L, with perfluorooctanoic acid (PFOA) as the dominant compound. Many emerging PFASs, including 6:2 fluorotelomer sulfonates (FTS), 6:2 chlorinated polyfluorinated ether sulfonic acid (Cl-PFESAs), ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA), and hexafluoropropylene oxide (HFPO) homologues were frequently detected in 96.7-100% of the samples. Multiple source apportionment analyses indicated that the PFASs in the groundwater mainly originated from industrial activities, but in rural areas, agricultural activities also contributed. The total oxidizable precursor (TOP) assay indicated that there were substantial unknown precursors of perfluoroalkyl acids (PFAAs) in the groundwater samples. The total concentration of PFASs decreased with the well depth, while the PFAA-precursors displayed contrasting vertical profile trends, which might be due to the suppressed microbial transformation in the groundwater. The potential human health risk caused by PFAS exposure via drinking groundwater in the Loess Plateau was low, except for one site that was close to the industry bases.


Assuntos
Fluorcarbonetos , Água Subterrânea , Poluentes Químicos da Água , Alcanossulfonatos , China , Monitoramento Ambiental , Fluorcarbonetos/análise , Humanos , Ácidos Sulfônicos , Poluentes Químicos da Água/análise
12.
Microvasc Res ; 133: 104075, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32950484

RESUMO

OBJECTIVE: Phosphorylation plays an essential role in the regulation of endothelial nitric oxide synthase (eNOS) activity. However, the phosphorylation of eNOS under hypoglycemia and whether hypoglycemia changes eNOS activity is unknown. This paper aims to clarify the regulation of eNOS phosphorylation and its activity change under hypoglycemia. METHODS: Bovine aortic endothelial cells (BAECs) and Sprague-Dawley rats were treated with hypoglycemia, and the phosphorylation of eNOS was subjected to western blot. Blood nitric oxide (NO) concentration was determined by NO kit and endothelial-dependent vasodilation was detected by multi-wire myograph. RESULTS: In both BAECs and rats' thoracic aorta, hypoglycemia induced eNOS phosphorylation decrease specifically on Threonine (Thr) 497. Inhibition of ubiquitination of protein kinase C α subunit (PKCα) reverses the decrease of eNOS phosphorylation in hypoglycemia. Ubiquitinated PKCα can be reversed by AMPK knockdown. In rats, insulin induced hypoglycemia increased the concentration of NO in arterial blood, and progressively enhanced the endothelium-dependent vasodilation of the thoracic and mesenteric aorta. CONCLUSIONS: In vitro, the activation of AMPK may lead to the expression of PKCα by regulating ubiquitination, resulting in a decrease in the level of P-eNOS Thr497 phosphorylation under hypoglycemia. In vivo, insulin-induced hypoglycemia produces a beneficial cardiovascular effect on rats.


Assuntos
Aorta Torácica/enzimologia , Células Endoteliais/enzimologia , Hipoglicemia/enzimologia , Artérias Mesentéricas/enzimologia , Óxido Nítrico Sintase Tipo III/metabolismo , Vasodilatação , Proteínas Quinases Ativadas por AMP/genética , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Aorta Torácica/fisiopatologia , Glicemia/metabolismo , Bovinos , Células Cultivadas , Modelos Animais de Doenças , Feminino , Hipoglicemia/fisiopatologia , Masculino , Artérias Mesentéricas/fisiopatologia , Óxido Nítrico/sangue , Fosforilação , Proteína Quinase C-alfa/genética , Proteína Quinase C-alfa/metabolismo , Ratos Sprague-Dawley , Transdução de Sinais
13.
Artigo em Inglês | MEDLINE | ID: mdl-33199390

RESUMO

Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1 The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.


Assuntos
Infecções por Klebsiella , Klebsiella pneumoniae , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , China , Humanos , Klebsiella , Infecções por Klebsiella/tratamento farmacológico , Klebsiella pneumoniae/genética , Testes de Sensibilidade Microbiana , Plasmídeos/genética
14.
Front Plant Sci ; 11: 304, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32265957

RESUMO

Cucurbits (Cucurbitaceae family) include many economically important fruit vegetable crops such as watermelon, pumpkin/squash, cucumber, and melon. Seed size (SS) is an important trait in cucurbits breeding, which is controlled by quantitative trait loci (QTL). Recent advances have deciphered several signaling pathways underlying seed size variation in model plants such as Arabidopsis and rice, but little is known on the genetic basis of SS variation in cucurbits. Here we conducted literature review on seed size QTL identified in watermelon, pumpkin/squash, cucumber and melon, and inferred 14, 9 and 13 consensus SS QTL based on their physical positions in respective draft genomes. Among them, four from watermelon (ClSS2.2, ClSS6.1, ClSS6.2, and ClSS8.2), two from cucumber (CsSS4.1 and CsSS5.1), and one from melon (CmSS11.1) were major-effect, stable QTL for seed size and weight. Whole genome sequence alignment revealed that these major-effect QTL were located in syntenic regions across different genomes suggesting possible structural and functional conservation of some important genes for seed size control in cucurbit crops. Annotation of genes in the four watermelon consensus SS QTL regions identified genes that are known to play important roles in seed size control including members of the zinc finger protein and the E3 ubiquitin-protein ligase families. The present work highlights the utility of comparative analysis in understanding the genetic basis of seed size variation, which may help future mapping and cloning of seed size QTL in cucurbits.

15.
Sci Total Environ ; 659: 188-199, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31096369

RESUMO

As part of the Energy Golden Triangle in northwest China and the largest coal-to-liquids industry in the world, the emission and contamination of fine particles in the Ningdong National Energy and Chemical Industrial Base (NECIB) are unknown. There are also large knowledge gaps in the association of air pollution with coal-to-liquids industry. This paper reports the chemical composition and source apportionment of PM1 and PM2.5 collected at two industrial sites Yinglite (YLT) and Baofeng (BF) from a field campaign during summer 2016 and winter 2017. Major chemical components in PM1 and PM2.5, including carbonaceous aerosols, water-soluble inorganic ions, and metal elements were analyzed. The Positive Matrix Factorization (PMF) model and the ISORROPIA II thermodynamic equilibrium model were used to track possible sources and contributions of these chemical components to the formation of the two fine particles. The results identified four primary sources of the fine particles, including vehicle emissions, biomass burning and waste incineration, the secondary aerosols and coal combustion, and soil dust. The PM1 and PM2.5 concentrations were higher in winter than summer. The summed secondary inorganic and carbonaceous aerosols accounted for 36.1-40.0% of PM2.5 mass. The total mass of chemical components identified in the source apportionment only explained about 64.2 to 72.4% of the PM2.5 mass. These results imply some missing sources in this large-scale coal chemical industry base. A coupled weather forecasting and atmospheric chemistry model WRF-Chem was employed to simulate the PM2.5 mass and concentrations of OC and EC, and to examine the origins of PM2.5 across the NECIB. The modeled concentrations of OC and EC were consistent with the sampled data, but the modeled mass of PM2.5 is lower considerably than the measurements, again suggesting unknown sources of fine particles in this energy industrial base.

16.
Liver Int ; 39(8): 1577-1586, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30963702

RESUMO

AIM: To evaluate individualized treatment strategy (ITS) and long-term outcomes of endovascular treatment of Budd-Chiari syndrome (BCS) with obstructed inferior vena cava (IVC) based on different degrees of hepatic vein (HV) involvement. METHODS: From January 2006 to June 2017, 456 consecutive patients with BCS with obstructed IVC underwent endovascular treatment with ITS. All patients received IVC recanalization. Then, 426 patients with at least one patent HV received no additional treatment. Twenty-fivepatients with membranous or segmental occlusion of HVs underwent HV recanalization and for the remaining five patients with diffuse HVs occlusion, a transjugular intrahepatic portosystemic shunt (TIPS) was performed. RESULTS: The endovascular treatment was technically successful in 455 of the 456 patients (99.8%). The complication rate was 5.0% (23/456), with major complications in 13 patients (2.8%) and minor complications in 10 patients (2.2%). Median follow-up time was 60.5 months (range, 4-120 months). The cumulative 1-, 2-, 5- and 10-year primary vessel patency rates were 93.6%, 89.9%, 80.5% and 74.3% respectively and the cumulative 1-, 2-, 5-, 10- year secondary patency rates were 99.8%, 99.8%, 98.2% and 97.2% respectively. The cumulative 1-, 2-, 5- and 10-year survival rates were 98.4%, 95.8%, 91.2% and 76.5% respectively. Illness duration and decreased serum albumin were independent predictors of survival. CONCLUSION: The ITS for Asian BCS with obstructed IVC and varying degrees of HV involvement appears to be effective and with good long-term outcomes.


Assuntos
Síndrome de Budd-Chiari/terapia , Adolescente , Adulto , Idoso , Síndrome de Budd-Chiari/mortalidade , Criança , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medicina de Precisão , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
17.
Environ Pollut ; 242(Pt B): 1353-1361, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30130716

RESUMO

This study conducted a field campaign to collect atmospheric deposition samples of heavy metals and arsenic, a metalloid element with typical chemical-physical characteristics (HMA), from 12 sampling sites and water samples from 37 rivers across the Bohai Sea (BS) and North Yellow Sea (NYS) in China. The HMA budgets in the BS and NYS were quantified by a budget model, which was developed based on the HMA inputs from atmospheric deposition and riverine discharge, sequestration to sediment, and interexchange among the BS's four subareas and the NYS. Statistical analyses of 76 deposition samples and 109 water concentration samples showed that atmospheric deposition was a main pathway of Pb entering the BS and NYS, whereas riverine discharge dominated the input of Cr, Cu, Zn, Cd, and As into the marine environment. Modeled results showed that the fractions of HMA in the water bodies compared with their total burdens were 86.6 ±â€¯4.55% in the Liaodong Bay, 60.5 ±â€¯10.5% in the Bohai Bay, 20.9 ±â€¯9.05% in the Laizhou Bay, 95.1 ±â€¯2.06% in the Central BS, and 94.3 ±â€¯1.93% in the NYS. The lowest fraction of HMA in the Laizhou Bay was attributed to high sedimentation rates and higher suspended particulate matter concentrations due to inputs from the Yellow River. The modeled 1-, 10- and 100- year mass budgets indicated that the Liaodong Bay in the north of the BS was a sink of HMA, the Bohai Bay and Laizhou Bay in the west and south of the BS acted as sources, and the Central BS and NYS were a transition area for most HMA.


Assuntos
Arsênio/análise , Metais Pesados/análise , Poluentes Químicos da Água/análise , Baías , China , Monitoramento Ambiental , Sedimentos Geológicos/análise , Modelos Teóricos , Material Particulado/análise , Rios
18.
Biochem Biophys Res Commun ; 499(3): 551-555, 2018 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-29596825

RESUMO

Circular RNA (circRNA), a family of RNA generated by RNA circularization, is ubiquitously expressed in tissues and possesses increasingly important biological functions. Hyperglycemia-induced endothelial dysfunction is an initiating event in the pathogenesis of diabetes-associated cardiovascular complications. How high glucose may affect circRNAs is unknown. To address this issue, human endothelial cells were exposed to high glucose treatment and the changes of circRNAs were measured by RNA sequencing. A total 3686 circRNAs, including 1040 previously unrecorded circRNAs, were detected; and 95 different expression (DE) circRNAs were observed. The host genes of these DE circRNAs were further studied by function enrichment analyses. These analyses revealed genes of phosphoproteins, transferases, and zine finger proteins. Since circRNAs can function as a microRNA (miRNA) sponge, circRNAs-miRNAs interaction networks were explored by bioinformatics. These analyses identified a number of miRNAs, which might interact with DE circRNAs and play roles in the actions of high glucose on endothelial cells. These results demonstrate that high glucose exposure profoundly changes circRNA expression in endothelial cells. Altered circRNA expression may contribute to the effects of high glucose on endothelial function in diabetes.


Assuntos
Células Endoteliais da Veia Umbilical Humana/metabolismo , Hiperglicemia/genética , Hiperglicemia/patologia , RNA/genética , Biologia Computacional , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , RNA/metabolismo , RNA Circular , Análise de Sequência de RNA
19.
Environ Pollut ; 233: 114-124, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29059626

RESUMO

Short-chain chlorinated paraffins (SCCPs) have been produced and emitted intensively around the Bohai Sea, potentially causing risks to this unique ecosystem and one of primary fishery resources in China and busiest seaways in the world. Little is known about fate, cycling, and sources of SCCPs in the Bohai Sea biotic and abiotic environment. In this study, we combined a marine food web model with a comprehensive atmospheric transport-multiple phase exchange model to quantify SCCPs in the biotic and abiotic environment in the Bohai Sea. We performed multiple modeling scenario investigations to examine SCCP levels in water, sediment, and phytoplankton. We assessed numerically dry and wet depositions, biomagnification and bioaccumulation of SCCPs in the Bohai Sea marine food web. Results showed declining SCCP levels in water and sediment with increasing distance from the coastline, and so do dry and wet depositions. The net deposition overwhelmed the water-air exchange of SCCPs due to their current use in China, though the diffusive gas deposition fluctuated monthly subject to mean wind speed and temperature. A risk assessment manifests that SCCPs levels in the Bohai Sea fish species are at present not posing risks to the residents in the Bohai Sea Rim region. We identified that the SCCP emission sources in the south of the Bohai Sea made a primary contribution to its loadings to the seawater and fish contamination associated with the East Asian summer monsoon. In contrast, the SCCP emissions from the north and northwest regions of the Bohai Sea were major sources contributing to their loading and contamination to Bohai Sea food web during the wintertime, potentially driven by the East Asian winter monsoon.


Assuntos
Monitoramento Ambiental , Parafina/análise , Poluentes Químicos da Água/análise , Animais , China , Ecossistema , Peixes , Cadeia Alimentar , Hidrocarbonetos Clorados/análise , Água do Mar
20.
Sci Total Environ ; 573: 1031-1040, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27607906

RESUMO

Daily PM2.5 samples were collected in 2014 at a suburban petrochemical industrial site and a downtown site in Lanzhou city, Northwest China. Major chemical components in PM2.5, including water-soluble ions, metal elements, and organic and elemental carbon (OC and EC) were determined. The chemical mass closure method and the ISORROPIA II thermodynamic equilibrium model were used to reconstruct PM2.5 mass and quantify the combinations of NH4+, SO42- and NO3- to PM2.5. Positive matrix factorization (PMF) model was employed to apportion potential sources of PM2.5. The annual average PM2.5 concentration was 93.7±49.6µgm-3 at the suburban petrochemical industrial site and 88.9±52.0µgm-3 at the urban site, with the highest seasonal average in winter and the lowest in summer at both sites. Mineral dust was identified as the highest contributor to PM2.5 in spring, while water-soluble inorganic ions and carbonaceous aerosols were the dominant chemical components in other seasons. The correlation relationships between OC and EC and between K+ and EC suggested that coal combustion and vehicle exhaust were the major sources of carbonaceous aerosols in Lanzhou. Six major sources were identified by the PMF model. Coal combustion, soil dust, traffic emissions, and secondary inorganic aerosols were the dominant contributors, together accounting for 82% of PM2.5 mass.


Assuntos
Poluentes Atmosféricos/análise , Poluentes Atmosféricos/química , Monitoramento Ambiental/métodos , Material Particulado/análise , Material Particulado/química , Aerossóis , Indústria Química , China , Clima Desértico , Poeira/análise , Incineração , Indústria de Petróleo e Gás , Tamanho da Partícula , Emissões de Veículos/análise
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