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1.
Medicine (Baltimore) ; 100(18): e25782, 2021 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-33950973

RESUMO

RATIONALE: Spindle cell lipoma is a rare, uncommon type of benign lipomatous tumor, a distinct group of lipomas composed of mature adipocytes, uniform spindle cells, and multinucleated giant cells associated with ropey collagen. Immunohistochemically, spindle cell lipoma is characterized by the diffuse expression of CD34. PATIENT CONCERNS: We present a rare case of a 56-year-old man who complained of vomiting out of a smooth and giant mass in the oral cavity provoked by an intra-abdominal pressure increase. Oral examination revealed an elongated mass protruding from the mouth. Computed tomography of the patient showed a mass from left pyriform to oral cavity, with 2.38 × 2.78 × 16.86 cm in size. The flexible fiberscope showed that the pedicle of the elongated mass originated from the posterior wall of the hypopharynx, corresponding to the left pyriform fossa. DIAGNOSIS: Histopathologically, the tumor was mainly composed of hyperplastic adipocytes, admixed with small blood vessels, and scattered inside adipose tissue spindle cells. The immunohistochemical profile revealed positivity of spindle cells for CD34, negativity for S100, and low proliferation with Ki67, which confirmed the diagnosis of spindle cell lipoma and revealed its benign behavior. INTERVENTIONS: The patient underwent hypopharyngeal mass resection using transoral suspension laryngoscopy. OUTCOMES: No recurrence was found after 5 months of follow-up. LESSONS: Spindle cell lipoma is difficult to diagnose early because of slow growth and subtle symptomatology. This entity should be differentiated from several benign or malignant subtypes of lipomas, including liposarcomas. In this case, the spindle cell lipoma is large and originates from the hypopharynx, which is a rare entity and presents with atypical symptoms. This case gave rise to further studies on the clinical and pathologic characteristics of this tumor in the future.

3.
J Cardiothorac Surg ; 16(1): 5, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33583412

RESUMO

OBJECTIVE: To explore the clinical effect of 3D printing combined with framework internal fixation technology on the minimally invasive internal fixation of high complex rib fractures. METHODS: Total 16 patients with high complex rib fractures were included in the study. Before the procedure, the 3D rib model was reconstructed based on the thin-layer chest CT scan. According to the 3D model, the rib locking plate was pre-shaped, and the preoperative planning were made including the direction of the locking plate, the location of each nail hole and the length of the screw. During the operation, the locking plate was inserted from the sternum to the outermost fracture lines of ribs with screws at both ends. In addition, the locking plate was used as the frame to sequentially reduce the middle fracture segment and fix with screws or steel wires. Chest x-rays or chest CT scans after surgery were used to assess the ribs recovery. All patients were routinely given non-steroidal anti-inflammatory drugs (NSAIDS) for analgesia, and the pain level was evaluated using numerical rating scale (NRS). RESULTS: The preoperative planning according to the 3D printed rib model was accurate. The reduction and fixation of each fracture segment were successfully completed through the framework internal fixation technology. No cases of surgical death, and postoperative chest pain was significantly alleviated. Five to 10 months follow up demonstrated neither loosening of screws, nor displacement of fixtures among patients. The lungs of each patients were clear and in good shape. CONCLUSION: The application of 3D printing combined with framework internal fixation technology to the high complex rib fractures is beneficial for restoring the inherent shape of the thoracic cage, which can realize the accurate and individualized treatment as well as reduces the operation difficulty.

4.
Aging (Albany NY) ; 13(3): 4388-4408, 2021 01 20.
Artigo em Inglês | MEDLINE | ID: mdl-33493133

RESUMO

Lumican (LUM), a small leucine-rich proteoglycan, is a component of the extracellular matrix. Abnormal LUM expression is potentially associated with cancer progression. In the present study, we confirmed high LUM mRNA expression in colorectal adenocarcinoma (COAD) through the UALCAN database. The Kaplan-Meier method, univariate, and multivariate COX analysis showed that high LUM expression is an independent determinant of poor prognosis in COAD. A COX regression model was constructed based on clinical information and LUM expression. The receiver operating characteristic (ROC) curve indicated that this model was highly accurate in monitoring COAD prognosis. The co-expression network of LUM was determined by LinkedOmics, which showed that LUM expression was closely related to immune escape and the miR200 family. Furthermore, we studied the co-expression network of LUM and found that LUM could promote tumor metastasis and invasion. The Tumor Immune Estimation Resource website showed that LUM was closely related to immune infiltration and correlated with regulatory T cells, tumour-associated macrophages, and dendritic cells. We found that LUM cultivated cancer progression by targeting the miR200 family to promote epithelial-to-mesenchymal transition. These findings suggest that LUM is a potential target for inhibiting immune escape and carcinogenic pathways.

5.
Technol Cancer Res Treat ; 19: 1533033820985787, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33371795

RESUMO

OBJECTIVE: This study aims to construct a systematic mRNA-miRNA-lncRNA network to identify novel lncRNAs and miRNAs biomarkers for laryngeal squamous cell carcinoma (LSCC). METHODS: The mRNA, miRNA and lncRNA expression profiles of LSCC were obtained from Gene Expression Omnibus (GEO) database. The differentially expressed mRNAs, miRNAs and lncRNAs (DEmRNAs, DEmiRNAs and DElncRNAs) were screened between LSCC tissues and controls. Functional analysis of DEmRNAs, DEmRNAs targeted by DEmiRNAs and DEmRNAs targeted by DElncRNAs were respectively performed. The miRWalk, starbase and DIANA-LncBase were respectively used to predict DEmiRNAs-DEmRNAs, DElncRNAs-DEmRNAs and DElncRNAs-DEmiRNAs pairs. ceRNA network was built by DEmiRNAs-DEmRNAs and DElncRNAs-DEmiRNAs pairs. LncRNA subcellular localization was predicted using lncLocator. Using published The Cancer Genome Atlas (TCGA) and external datasets (GSE127165 and GSE133632), we also validated the expression of key DElncRNAs and DEmiRNAs in ceRNA network. The diagnostic and prognostic value of candidate genes was evaluated by ROC curve analysis and survival analysis, respectively. RESULTS: There were 5 mRNA datasets, 3 miRNA datasets and 2 lncRNA datasets in this study. Totally, 2957 DEmRNAs, 61 DElncRNAs and 23 DEmiRNAs were identified. Functional analysis of DEmRNAs shows that they were significantly enriched in cancer-related pathways, such as DNA replication and extracellular matrix organization. There were 11 DEmiRNAs, 17 DElncRNAs and 967 DEmRNAs in the ceRNA network. Notably, up-regulated lncRNA DGCR5-down-regulated has-miR-338-3p/has-miR-139-5p pairs in this network were experimentally validated. Moreover, down-regulated AL121839.2, down-regulated LINC02147, up-regulated AC079328.2, up-regulated AC004943.2 and up-regulated HMGA2-AS1 were located in the cytoplasm. AL121839.2 and LINC02147 interacted with has-miR-1246. AC004943.2, AC079328.2 and HMGA2-AS1 targeted has-miR-3185, has-miR-3137 and has-miR-582-5p, respectively. Based on the TCGA and external datasets (GSE127165 and GSE133632), DGCR5 and AC004943.2 were significantly up-regulated while AL121839.2 and LINC02147, has-miR-338-3p, has-miR-139-5p and has-miR-582-5p were significantly down-regulated, which were consistent with our integration analysis. DGCR5, AL121839.2, LINC02147, AC004943.2, has-miR-338-3p, has-miR-139-5p and has-miR-582-5p could predict the occurrence of LSCC. Survival analysis suggested that only, AL121839.2 has potential prognostic value for LSCC. CONCLUSION: This study provided novel insights into the ceRNA network and uncovered novel lncRNAs and miRNAs with diagnostic value in LSCC.

6.
World J Stem Cells ; 12(7): 676-687, 2020 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-32843921

RESUMO

BACKGROUND: Lymphedema is a chronic, debilitating and incurable disease that affects 0.13%-2% of the global population. Emerging evidence indicates that adipose-derived stem cells (ADSCs) might serve as suitable seed cells for lymphatic tissue engineering and lymphedema therapy. AIM: To summarize applications of ADSCs for treating lymphedema in both animal studies and clinical trials. METHODS: A systematic search was performed on four databases - PubMed, Clinicaltrials.gov, the evidence-based Cochrane Library, and OVID - using the following search string: ("lymphedema" or "lymphoedema" or "lymphangiogenesis") and ("adipose-derived stem cells" or "adipose-derived stromal cells" or "adipose-derived regenerative cells"). A manual search was performed by skimming the references of relevant studies. Animal studies and clinical trials using adipose-derived cells for the treatment of any kind of lymphedema were included. RESULTS: A total of eight research articles published before November 2019 were included for this analysis. Five articles focused on animal studies and another three focused on clinical trials. ADSC transplantation therapy was demonstrated to be effective against lymphedema in all studies. The animal studies found that coadministration of ADSCs and controlled-release vascular endothelial growth factor-C or platelet-rich plasma could improve the effectiveness of ADSC therapy. Three sequential clinical trials were conducted on breast cancer-related lymphedema patients, and all showed favorable results. CONCLUSION: ADSC-based therapy is a promising option for treating lymphedema. Large-scale, multicenter randomized controlled trials are needed to develop more effective and durable therapeutic strategies.

7.
Angew Chem Int Ed Engl ; 59(43): 19095-19101, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-32686265

RESUMO

In this work, an artificial electrode/electrolyte (E/E) interface, made by coating the electrode surface with a quaternary ammonium cation (R4 N+ ) surfactant, was successfully developed, leading to a change in the CO2 reduction reaction (CO2 RR) pathway. This artificial E/E interface, with high CO2 permeability, promotes CO2 transportation and hydrogenation, as well as suppresses the hydrogen evolution reaction (HER). Linear and branched surfactants facilitated formic acid and CO production, respectively. Molecular dynamics simulations show that the artificial interface provided a facile CO2 diffusion pathway. Moreover, density-functional theory (DFT) calculations revealed the stabilization of the key intermediate, OCHO*, through interactions with R4 N+ . This strategy might also be applicable to other electrocatalytic reactions where gas consumption is involved.

8.
Front Immunol ; 11: 1187, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32695099

RESUMO

The butyrophilins (BTNs) represent a unique family of immunoglobulin. They were considered to be involved in milk lactation after their discovery in 1981. With the development of research, an increasing number of research revealed that BTNs play important roles in immune regulation [1992-2019]. Our research aimed to summarize the BTN research status and their relationship with lung cancers and breast cancers by bibliometrics and bioinformatics methods. Our results indicate that the researches on immune-regulatory functions of BTNs gradually developed from 1992 to 2006, whereas they increased quickly after 2007. There are international cooperations among 56 countries, of which the United States is the most active one with the highest number of studies as well as highest citations. By coauthorship and cocitation analysis, we showed that Adrian Hayday, who is active in γδ T-cell field, was an active author in BTN publications with average year of 2015 and led a subfield. By keywords co-occurrence analysis, we found that γδ T cell, which is an important cancer immune regulator, is one important hotspot. Finally, we found that several BTN members' expression levels were significantly correlated with prognosis of lung cancer and breast cancer patients. Thus, these BTNs might play immune regulatory effects and could serve as potential biomarkers for cancer.

9.
Int J Mol Med ; 46(3): 936-948, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32705240

RESUMO

Cardiovascular diseases (CVDs), such as atherosclerosis, hypertension, myocardial infarction and diabetic heart disease, are associated with high morbidity and mortality rates worldwide, and may also induce multiple organ failure in their later stages, greatly reducing the long­term survival of the patients. There are several causes of CVDs, but after nearly a decade of investigation, researchers have found that CVDs are usually accompanied by an imbalance of gut microbiota and a decreased abundance of flora. More importantly, the metabolites produced by intestinal flora, such as trimethylamine and trimethylamine N­oxide, bile acids, short­chain fatty acids and aromatic amino acids, exert different effects on the occurrence and development of CVDs, as observed in the relevant pathways in the cells, which may either promote or protect against CVD occurrence. It is known that changes in the intestinal flora following antibiotic administration, diet supplementation with probiotics, or exercise, can interfere with the composition of the intestinal flora and may represent an effective approach to preventing or treating CVDs. The focus of this review was the analysis of gut microbiota metabolites to elucidate their effects on CVDs and to identify the most cost­effective and beneficial methods for treating CVDs with minimal side effects.

10.
Plant Cell ; 32(9): 2823-2841, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32699171

RESUMO

Zeins are the predominant storage proteins in maize (Zea mays) seeds, while Opaque2 (O2) is a master transcription factor for zein-encoding genes. How the activity of O2 is regulated and responds to external signals is yet largely unknown. Here, we show that the E3 ubiquitin ligase ZmRFWD3 interacts with O2 and positively regulates its activity by enhancing its nuclear localization. Ubiquitination of O2 enhances its interaction with maize importin1, the α-subunit of Importin-1 in maize, thus enhancing its nuclear localization ability. We further show that ZmRFWD3 can be phosphorylated by a Suc-responsive protein kinase, ZmSnRK1, which leads to its degradation. We demonstrated that the activity of O2 responds to Suc levels through the ZmSnRK1-ZmRFWD3-O2 signaling axis. Intriguingly, we found that Suc levels, as well as ZmRFWD3 levels and the cytonuclear distribution of O2, exhibit diurnal patterns in developing endosperm, leading to the diurnal transcription of O2-regulated zein genes. Loss of function in ZmRFWD3 disrupts the diurnal patterns of O2 cytonuclear distribution and zein biosynthesis, and consequently changes the C/N ratio in mature seeds. We therefore identify a SnRK1-ZmRFWD3-O2 signaling axis that transduces source-to-sink signals and coordinates C and N assimilation in developing maize seeds.

11.
Nanoscale Res Lett ; 15(1): 147, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32661746

RESUMO

With the demand for higher energy density and smaller size lithium-ion batteries (LIBs), the development of high specific capacity active materials and the reduction of the usage of inactive materials are the main directions. Herein, a universal method is developed for binder-free electrodes for excellent stable LIBs by rolling the electrospun membrane directly onto the commercial current collector. The rolling process only makes the fiber web denser without changing the fiber structure, and the fiber web still maintains a porous structure. This strategy significantly improves the structural stability of the membrane compared to the direct carbonized electrospun membrane. Moreover, this method is suitable for a variety of polymerizable adhesive polymers, and each polymer can be composited with different polymers, inorganic salts, etc. The electrode prepared by this method can be stably cycled for more than 2000 cycles at a current density of 2500 mA g-1. This study provides a cost-effective and versatile strategy to design the LIB electrode with high energy density and stability for experimental research and practical application.

12.
Medicine (Baltimore) ; 99(24): e20549, 2020 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-32541477

RESUMO

BACKGROUND: Despite rapid reports on the correlation between body mass index (BMI) and periprosthetic joint infection (PJI) after total joint arthroplasty, some have conducted regression tests or meta-analyses with controversial results. In this study, we systematically meta-analyzed relevant trials and carefully evaluated the correlation for verification. METHODS: Literature on the correlation between BMI and PJI following total joint arthroplasty was retrieved in PubMed, Embase and Cochrane Library due September 2019. Stata 13.0 software was adopted for data synthesis and analyses of publication bias and sensitivity. Random-effect models were used to summary the overall estimate of the multivariate adjusted odds ratio (OR)/hazard ratio/rate ratio with 95% confidence intervals (CIs). RESULTS: A total of 29 observational studies representing 3,204,887 patients were included. The meta-analysis revealed that the risk of postoperative PJI significantly increased by 1.51 times in the obese group (OR = 1.51; 95% CI = 1.30-1.74 for the obese group vs. the non-obese group), and by 3.27 times in the morbid obese group (OR = 3.27; 95% CI = 2.46-4.34 for the morbid obese group vs the non-morbid obese group). A significant association remained consistent, as indicated by subgroup analyses and sensitivity analyses. CONCLUSION: Our findings demonstrate that postoperative PJI is positively correlated with BMI, with obese patients showing a greater risk of developing PJI than non-obese patients. Similarly, morbid obese patients present a higher risk of PJI than non-morbid obese patients. However, this conclusion needs to be corroborated by more prospective studies.


Assuntos
Artrite Infecciosa/etiologia , Índice de Massa Corporal , Obesidade/complicações , Infecções Relacionadas à Prótese/etiologia , Humanos , Metanálise como Assunto , Revisões Sistemáticas como Assunto
13.
Sci Adv ; 6(21): eaay5098, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32494732

RESUMO

In lithium-sulfur (Li-S) chemistry, the electrically/ionically insulating nature of sulfur and Li2S leads to sluggish electron/ion transfer kinetics for sulfur species conversion. Sulfur and Li2S are recognized as solid at room temperature, and solid-liquid phase transitions are the limiting steps in Li-S batteries. Here, we visualize the distinct sulfur growth behaviors on Al, carbon, Ni current collectors and demonstrate that (i) liquid sulfur generated on Ni provides higher reversible capacity, faster kinetics, and better cycling life compared to solid sulfur; and (ii) Ni facilitates the phase transition (e.g., Li2S decomposition). Accordingly, light-weight, 3D Ni-based current collector is designed to control the deposition and catalytic conversion of sulfur species toward high-performance Li-S batteries. This work provides insights on the critical role of the current collector in determining the physical state of sulfur and elucidates the correlation between sulfur state and battery performance, which will advance electrode designs in high-energy Li-S batteries.

14.
J Fluoresc ; 30(4): 907-915, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32494937

RESUMO

Dendrobium officinale is a rare and endangered perennial herb in China, which have been used in preparing Chinese tonic medicine for hundreds of years. The severe shortage of this herb and high price have caused that many similar plants were processed as an adulterant and it became difficult to distinguish genuine D. officinale by traditional authentication methods. A sensitive, convenient, and specific method for rapid identification of D. officinale is urgently needed. In the present study, 3D front-face fluorescence technique merged with Independent Component Analysis was used to get the "pure" independent fluorescence signals. The overall 3D-FFF spectra were decomposed into seven independent components (IC). To distinguish D. officinale from other species, IC1 and IC4 were chosen as fluorescence markers and the fluorescence intensity (FI) value at 340 nm/ 442 nm (excitation /emission wavelength) of IC1and 315 nm/ 468 nm of IC4 were used to build a linear model for identifying D. officinale successfully. Compared with other Dendrobium species, D. officinale showed much higher FI1 and FI4 value which is a direct criterion for identification. Meanwhile, even though the FI values of D. officinale may fluctuate due to the difference of growing conditions, the relation between FI1 and FI4 amazingly always fit the linear model constructed (FI4 = 9.9046 + 0.6119FI1, R2 = 0.9811). The linear model is an important finding and specific for D. officinale. Based on the FI value and the goodness of fit in the linear model, D. officinale can be identified quickly.

15.
Nanoscale Res Lett ; 15(1): 107, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32405875

RESUMO

Lithium-ion batteries (LIBs) are currently the most important energy storage system. Separators in the battery play a critical role in terms of the rate capability, cycle life, and safe operation. However, commercial separators exhibit poor electrolyte wettability and limited safety. It is also extremely important to eliminate the hazardous small molecules (e.g., H2O and HF) inside the battery to enhance the service life. Herein, a functionalized poly(vinylidene fluoride-co-hexafluoropropylene)@polyacrylonitrile (PVDF-HFP@PAN) separator modified by 4-Å molecular sieves (MS) was fabricated by hydrothermal method for LIBs. MS@PVDF-HFP@PAN separator exhibits high thermal stability and carbonate electrolyte wettability. In addition, it can lower the moisture value in the battery system to 13 ppm, which significantly improves the electrolyte quality. When the current density increased from 0.2 to 5 C, the discharging capacity of the cell with MS@PVDF-HFP@PAN declines from 177.6 to 143.2 mAh g-1, demonstrating an excellent capacity retention of 80.6%. The discharge capacity retention of NMC622 half-cell with MS@PVDF-HFP@PAN after 100 cycles is 98.6% of its initial discharge capacity, which is higher than that of a cell with the Celgard 2400 separator (91.9%).

16.
Nanoscale Res Lett ; 15(1): 112, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32424777

RESUMO

Lithium-ion batteries (LIB) as energy supply and storage systems have been widely used in electronics, electric vehicles, and utility grids. However, there is an increasing demand to enhance the energy density of LIB. Therefore, the development of new electrode materials with high energy density becomes significant. Although many novel materials have been discovered, issues remain as (1) the weak interaction and interface problem between the binder and the active material (metal oxide, Si, Li, S, etc.), (2) large volume change, (3) low ion/electron conductivity, and (4) self-aggregation of active materials during charge and discharge processes. Currently, the binder-free electrode serves as a promising candidate to address the issues above. Firstly, the interface problem of the binder and active materials can be solved by fixing the active material directly to the conductive substrate. Secondly, the large volume expansion of active materials can be accommodated by the porosity of the binder-free electrode. Thirdly, the ion and electron conductivity can be enhanced by the close contact between the conductive substrate and the active material. Therefore, the binder-free electrode generally exhibits excellent electrochemical performances. The traditional manufacture process contains electrochemically inactive binders and conductive materials, which reduces the specific capacity and energy density of the active materials. When the binder and the conductive material are eliminated, the energy density of the battery can be largely improved. This review presents the preparation, application, and outlook of binder-free electrodes. First, different conductive substrates are introduced, which serve as carriers for the active materials. It is followed by the binder-free electrode fabrication method from the perspectives of chemistry, physics, and electricity. Subsequently, the application of the binder-free electrode in the field of the flexible battery is presented. Finally, the outlook in terms of these processing methods and the applications are provided.

17.
ACS Nano ; 14(4): 4559-4566, 2020 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-32271535

RESUMO

Performance of 2D photodetectors is often predominated by charge traps that offer an effective photogating effect. The device features an ultrahigh gain and responsivity, but at the cost of a retarded temporal response due to the nature of long-lived trap states. In this work, we devise a gain mechanism that originates from massive charge puddles formed in the type-II 2D lateral heterostructures. This concept is demonstrated using graphene-contacted WS2 photodetectors embedded with WSe2 nanodots. Upon light illumination, photoexcited carriers are separated by the built-in field at the WSe2/WS2 heterojunctions (HJs), with holes trapped in the WSe2 nanodots. The resulting WSe2 hole puddles provide a photoconductive gain, as electrons are recirculating during the lifetime of holes that remain trapped in the puddles. The WSe2/WS2 HJ photodetectors exhibit a responsivity of 3 × 102 A/W with a gain of 7 × 102 electrons per photon. Meanwhile, the zero-gate response time is reduced by 5 orders of magnitude as compared to the prior reports for the graphene-contacted pristine WS2 monolayer and WS2/MoS2 heterobilayer photodetectors due to the ultrafast intralayer excitonic dynamics in the WSe2/WS2 HJs.

18.
Anal Bioanal Chem ; 412(8): 1785-1794, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32052065

RESUMO

The prevalence of allergic rhinitis (AR) is increasing worldwide. However, the current systems used to measure levels of immunoglobulin E (IgE) in sera are associated with several disadvantages that limit their further application. Consequently, there is a need to develop novel highly sensitive strategies that can rapidly detect IgE in a quantitative manner. The development of such systems will significantly enhance our ability to diagnose, treat, and even prevent AR. Herein, we describe our experience of using quantum dot-based lateral flow immunoassay (QD-LFIA), combined with a portable fluorescence immunoassay chip detector (PFICD), to detect serum-specific IgE against Dermatophagoides pteronyssinus (Der-p) and Dermatophagoides farinae (Der-f), two common mite allergens in China. Our data showed that our system could detect serum-specific levels of IgE against Der-p and Der-f as low as 0.093 IU/mL and 0.087 IU/mL, respectively. We also established a standard curve to determine serum-specific IgE concentrations that correlated well with the clinical BioIC microfluidics system. The sensitivity of our assay was 96.7% for Der-p and 95.5% for Der-f, while the specificity was 87.2% for Der-p and 85.3% for Der-f. Collectively, our results demonstrate that QD-LFIA is a reliable system that could be applied to detect serum-specific IgE in accordance with clinical demands. This QD-LFIA strategy can be applied at home, in hospitals, and in pharmacies, with reduced costs and time requirements when compared with existing techniques. In the future, this system could be developed to detect other types of allergens and in different types of samples (for example, whole blood). Graphical abstract We describe our experiment using a quantum dot-based lateral flow immunoassay combined with a portable fluorescence immunoassay chip detector for both qualitative and quantitative detection of serum-specific IgE against two common mite allergens. This strategy can be applied at home, in hospitals, and in pharmacies, with reduced costs and time requirements. In the future, this system could be developed to detect other types of allergens and in different types of samples.


Assuntos
Alérgenos/sangue , Imunoensaio/métodos , Imunoglobulina E/sangue , Pontos Quânticos , Rinite Alérgica/sangue , Humanos , Limite de Detecção , Sistemas Automatizados de Assistência Junto ao Leito , Rinite Alérgica/imunologia
19.
ACS Nano ; 14(1): 985-992, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31904930

RESUMO

The most pressing barrier for the development of advanced electronics based on two-dimensional (2D) layered semiconductors stems from the lack of site-selective synthesis of complementary n- and p-channels with low contact resistance. Here, we report an in-plane epitaxial route for the growth of interlaced 2D semiconductor monolayers using chemical vapor deposition with a gas-confined scheme, in which patterned graphene (Gr) serves as a guiding template for site-selective growth of Gr-WS2-Gr and Gr-WSe2-Gr heterostructures. The Gr/2D semiconductor interface exhibits a transparent contact with a nearly ideal pinning factor of 0.95 for the n-channel WS2 and 0.92 for the p-channel WSe2. The effective depinning of the Fermi level gives an ultralow contact resistance of 0.75 and 1.20 kΩ·µm for WS2 and WSe2, respectively. Integrated logic circuits including inverter, NAND gate, static random access memory, and five-stage ring oscillator are constructed using the complementary Gr-WS2-Gr-WSe2-Gr heterojunctions as a fundamental building block, featuring the prominent performance metrics of high operation frequency (>0.2 GHz), low-power consumption, large noise margins, and high operational stability. The technology presented here provides a speculative look at the electronic circuitry built on atomic-scale semiconductors in the near future.

20.
Biomolecules ; 10(1)2020 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-31963204

RESUMO

Since the first discovery in 1961, more than 1300 ent-kaurane diterpenoids have been isolated and identified from different plant sources, mainly the genus Isodon. Chemically, they consist of a perhydrophenanthrene subunit and a cyclopentane ring. A large number of reports describe the anticancer potential and mechanism of action of ent-kaurane compounds in a series of cancer cell lines. Oridonin is one of the prime anticancer ent-kaurane diterpenoids that is currently in a phase-I clinical trial in China. In this review, we have extensively summarized the anticancer activities of ent-kaurane diterpenoids according to their plant sources, mechanistic pathways, and biological targets. Literature analysis found that anticancer effect of ent-kauranes are mainly mediated through regulation of apoptosis, cell cycle arrest, autophagy, and metastasis. Induction of apoptosis is associated with modulation of BCL-2, BAX, PARP, cytochrome c, and cleaved caspase-3, -8, and -9, while cell cycle arrest is controlled by cyclin D1, c-Myc, p21, p53, and CDK-2 and -4. The most common metastatic target proteins of ent-kauranes are MMP-2, MMP-9, VEGF, and VEGFR whereas LC-II and mTOR are key regulators to induce autophagy.

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