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1.
Int. j. morphol ; 37(3): 1085-1088, Sept. 2019. tab
Artigo em Inglês | LILACS-Express | ID: biblio-1012400

RESUMO

SUMMARY: With the accumulation of teaching experience and the summary of the teaching process in the teaching of medical colleges and universities, the course "Normal Human Morphology" has been basically on the right track in undergraduate education. However, most of the colleges and universities in China still use the traditional teaching mode, and the evaluation of students' learning effects and teacher teaching still follows the method of final evaluation. This method is not conducive to students' timely understanding of self-stage learning effects. It will affect the teacher's adjustment (or solution) to the specific links (or problems) that appear in the teaching process. The establishment of the mixed teaching model and formative evaluation system can solve the problems of the two to some extent.


RESUMEN: Con la mayor experiencia de los docentes y del proceso de aprendizaje en la enseñanza de facultades y universidades de medicina, el curso "Morfología Humana Normal" básicamente ha seguido una metodología correcta en la educación de pregrado. Sin embargo, la mayoría de los colegios y universidades en China aún utilizan el modelo de enseñanza tradicional, por lo cual, la evaluación de los efectos de aprendizaje de los estudiantes junto con la enseñanza docente, a la fecha, sigue el método de una evaluación final. Este método no es propicio para la comprensión oportuna por parte de los alumnos, en la etapa del auto-aprendizaje, ya que afecta la adaptación (o solución) del profesor a los enlaces (o problemas) específicos que aparecen en el proceso de enseñanza. El establecimiento de un modelo de enseñanza mixta y un sistema de evaluación formativa en cierta medida podrían resolver ambos problemas.

2.
Nan Fang Yi Ke Da Xue Xue Bao ; 39(3): 364-368, 2019 Mar 30.
Artigo em Chinês | MEDLINE | ID: mdl-31068313

RESUMO

OBJECTIVE: To analyze the clinical features of chronic myeloid leukemia (CML) with T315 I mutation (CML-T315I) and compare the effectiveness of different treatments. METHODS: We retrospectively analyzed the clinical data and outcomes of 19 patients with CML-T315I receiving different treatments. The T315 I mutations in these patients were detected by examination of BCR-ABL kinase domain (KD) mutation by RTQ-PCR and Sanger sequencing. The relapse following the treatments, defined as hematological, cytogenetic and molecular biological recurrences, were analyzed in these patients. RESULTS: Of the 19 patients with CML-T315I, 14 (73.7%) were in CML-CP stage at the initial diagnosis, and 13 (81.2%) were high-risk patients based on the Sokal scores. All the 19 patients were treated with TKI after the initial diagnosis, and during the treatment, 15 (78.9%) patients were found to have additional chromosomal aberrations, and 10 (52.6%) had multiple mutations; 13 (68.4%) of the patients experienced disease progression (accelerated phase/blast crisis) before the detection of T315I mutation, with a median time of 40 months (5-120 months) from the initial diagnosis to the mutation detection. After detection of the mutation, 12 patients were treated with ponatinib and 7 were managed with the conventional chemotherapy regimen, and their overall survival rates at 3 years were 83.3% and 14.2%, respectively (P < 0.001). CONCLUSIONS: CML patients resistant to TKI are more likely to have T315I mutations, whose detection rate is significantly higher in the progressive phase than in the chronic phase. These patients often have additional chromosomal aberrations and multiple gene mutations with poor prognoses and a high recurrence rate even after hematopoietic stem cell transplantation. Long-term maintenance therapy with ponatinib may improve the prognosis and prolong the survival time of the patients.


Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva , Resistencia a Medicamentos Antineoplásicos , Proteínas de Fusão bcr-abl , Humanos , Imidazóis , Mutação , Piridazinas , Estudos Retrospectivos
3.
Neurosci Res ; 2018 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-30452948

RESUMO

Accumulating evidence from preclinical and clinical studies indicates prenatal exposure to stress or excess glucocorticoids can affect offspring brain. HDAC2 is an important target of glucocorticoid. Here we detected HDAC2 expression in male offspring hippocampus from gestational restraint stressed rat during development and the relationship between HDAC2 expression and behaviors and neurogenesis in male offspring. Pregnant rats received restrained stress during the last week of pregnancy. Expressions of HDAC2 in offspring hippocampus were detected on postnatal 0 day (P0) and 60 days (P60). Neurogenesis was evaluated by Doublecortin (DCX) staining on P60. Anxiety-like behavior and cognition were detected in open field, elevated plus maze, novel object recognition test, and Barnes maze. We found that HDAC2 expression in the hippocampus of male prenatally stressed offspring (MPSO) was similar to the male control offspring on P0, but significantly lower on P60. Corresponding to the decreased expression of HDAC2 in MPSO hippocampus at P60, neurogenesis in the dentate gyrus of MPSO was significantly lower than the control male offspring. And MPSO also showed greater anxiety and poorer learning and memories abilities than control male offspring. These showed that HDAC2 could partly explain the effects of gestational stress on male offspring behaviors.

4.
PLoS One ; 12(9): e0185388, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28953973

RESUMO

Many studies on retinal injury and repair following elevated intraocular pressure suggest that the survival ratio of retinal neurons has been improved by various measures. However, the visual function recovery is far lower than expected. The homeostasis of retinal synapses in the visual signal pathway is the key structural basis for the delivery of visual signals. Our previous studies found that complicated changes in the synaptic structure between retinal neurons occurred much earlier than obvious degeneration of retinal ganglion cells in rat retinae. The lack of consideration of these earlier retinal synaptic changes in the rescue strategy may be partly responsible for the limited visual function recovery with the types of protective methods for retinal neurons used following elevated intraocular pressure. Thus, research on the modulatory mechanisms of the synaptic changes after elevated intraocular pressure injury may give new light to visual function rescue. In this study, we found that thrombospondin 2, an important regulator of synaptogenesis in central nervous system development, was distributed in retinal macroglia cells, and its receptor α2δ-1 was in retinal neurons. Cell cultures including mixed retinal macroglia cells/neuron cultures and retinal neuron cultures were exposed to elevated hydrostatic pressure for 2 h. The expression levels of glial fibrillary acidic protein (the marker of activated macroglia cells), thrombospondin 2, α2δ-1 and presynaptic proteins were increased following elevated hydrostatic pressure in mixed cultures, but the expression levels of postsynaptic proteins were not changed. SiRNA targeting thrombospondin 2 could decrease the upregulation of presynaptic proteins induced by the elevated hydrostatic pressure. However, in retinal neuron cultures, elevated hydrostatic pressure did not affect the expression of presynaptic or postsynaptic proteins. Rather, the retinal neuron cultures with added recombinant thrombospondin 2 protein upregulated the level of presynaptic proteins. Finally, gabapentin decreased the expression of presynaptic proteins in mixed cultures by blocking the interaction of thrombospondin 2 and α2δ-1. Taken together, these results indicate that activated macroglia cells may participate in alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure, and macroglia-derived thrombospondin 2 may modulate these changes via binding to its neuronal receptor α2δ-1.


Assuntos
Pressão Hidrostática , Neuroglia/metabolismo , Terminações Pré-Sinápticas/metabolismo , Neurônios Retinianos/metabolismo , Trombospondinas/metabolismo , Animais , Canais de Cálcio/metabolismo , Proteínas de Transporte/metabolismo , Células Cultivadas , Proteína 4 Homóloga a Disks-Large , Proteína Glial Fibrilar Ácida/metabolismo , Proteínas de Arcabouço Homer/metabolismo , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas de Membrana/metabolismo , Ratos Sprague-Dawley , Sinapsinas/metabolismo , Sinaptofisina/metabolismo
5.
Clin Lymphoma Myeloma Leuk ; 15(12): 790-6, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26482108

RESUMO

BACKGROUND: The monosomal karyotype (MK) is a well-known adverse prognostic factor and has been found to be related to poor outcome in patients with acute myeloid leukemia (AML). However, the outcome in MK-positive AML patients undergoing different therapies has not been well investigated. PATIENTS AND METHODS: We retrospectively analyzed clinical and laboratory features in 225 MK-positive AML patients. Clinical outcome of overall survival (OS) and disease-free survival (DFS) was evaluated in patients according to age group and in patients who received different therapy protocols. RESULTS: The proportion of MK-positive patients increased along with age. Also, patients who were treated with high-dose cytarabine (HD-Ara-C) as consolidation therapy followed by allogeneic hematopoietic stem cell transplantation (allo-HSCT) demonstrated longer OS and DFS compared to allo-HSCT or HD-Ara-C alone. Patients treated with allo-HSCT alone exhibited longer DFS compared to patients treated with HD-Ara-C alone. No difference in OS was discovered between these 2 single protocols. CONCLUSION: MK was associated with a lower complete remission rate. HD-Ara-C therapy followed by allo-HSCT could improve the prognosis of MK-positive AML patients.


Assuntos
Leucemia Mieloide Aguda/terapia , Adolescente , Adulto , Terapia Combinada , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Cariótipo , Leucemia Mieloide Aguda/genética , Leucemia Mieloide Aguda/mortalidade , Masculino , Pessoa de Meia-Idade , Monossomia , Prognóstico , Modelos de Riscos Proporcionais , Fatores de Risco , Resultado do Tratamento , Adulto Jovem
6.
Dis Markers ; 2015: 382186, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25944974

RESUMO

Mixed phenotype acute leukemia (MPAL) is a complex entity expressing both lymphoid and myeloid immunophenotyping. In the present study, 47 MPAL, 60 lymphoid antigen-positive acute myeloid leukemia (Ly(+)AML), and 90 acute myeloid leukemia with common myeloid immunophenotype (Ly(-)AML) patients were investigated. We found that, in MPAL patients, there were high proportions of blast cells in bone marrow and incidence of hepatosplenomegaly, lymphadenopathy, and Philadelphia chromosome. The overall survival (OS) and relapse-free survival (RFS) in MPAL patients were significantly shorter than those in Ly(+)AML and Ly(-)AML. With regard to the patients with normal karyotype only, the OS and RFS of MPAL were significantly lower than those of the Ly(+)AML and Ly(-)AML; but there were no significant differences in OS and RFS among the patients with complex karyotype. The OS rates of 3 groups with complex karyotype were lower than those of patients with normal karyotype. In Cox multivariate analysis, complex karyotype was an independent pejorative factor for both OS and RFS. Therefore, MPAL is confirmed to be a poor-risk disease while Ly(+)AML does not impact prognosis. Complex karyotype is an unfavorable prognosis factor in AML patients with different immunophenotype. Mixed immunophenotype and complex karyotype increase the adverse risk when they coexist.


Assuntos
Antígenos CD/imunologia , Antígenos Ly/imunologia , Cariótipo , Leucemia Aguda Bifenotípica/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Células da Medula Óssea/patologia , Criança , Pré-Escolar , China , Feminino , Humanos , Imunofenotipagem , Leucemia Aguda Bifenotípica/genética , Leucemia Aguda Bifenotípica/imunologia , Masculino , Pessoa de Meia-Idade , Análise de Sobrevida
7.
Zhonghua Xue Ye Xue Za Zhi ; 35(8): 703-7, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25152116

RESUMO

OBJECTIVE: To analyze the association of different types of ABL tyrosine point mutations and imatinib resistance to probe the relation between ABL tyrosine point mutations and the prognosis of patients with chronic myeloid leukemia (CML). METHODS: Nested reverse transcriptasepolym erase chain reaction was performed on samples from 70 patients to amplify the ABL kinase domain. Then, the amplified product was purified and sequenced in both direction. The homologous analysis was performed in combination of clinical data. RESULTS: The ABL domain point mutations were detected in 32 patients (45.7%) including 16 patients in chronic phase (CP), 6 patients in accelerated phase(AP)and 10 patients in blast phase (BP), which were detected as T315I, E255K, C475Y, Y253H, G321W, G250E, F317L, E258K, F359V, E459K and F311I, respectively. Sokal score with intermediate and high risk and Ph+ chromosome with complex karyotype were important risk factors for ABL domain point mutations. The 5-year overall survival (OS) was not significantly different between the patients with or without ABL domain point mutations (78.1% vs 84.2%, P=0.985), while the 5-year cumulative event-free survival (EFS) of two groups were 34.4% and 68.4% (P=0.034), respectively. The rate of complete cytogenetic response was higher in patients treated with allogenic hematopetic stem cell transplantation (allo-HSCT) compared with patients merely treated with second-generation tyrosine kinase inhibitors or chemotherapeutics (P=0.001). CONCLUSION: Patients with ABL domain point mutations had poor efficacy and prognosis compared to those without ABL domain point mutations. Detection of ABL domain point mutations in CML-CP was helpful for the adjustment of therapeutic options and improvement of prognosis. And allo-HSCT was a more effective therapy for patients with advanced phase.


Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva/genética , Mutação Puntual , Proteínas Proto-Oncogênicas c-abl/genética , Adolescente , Adulto , Idoso , Benzamidas/uso terapêutico , Criança , Resistencia a Medicamentos Antineoplásicos , Feminino , Humanos , Mesilato de Imatinib , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Piperazinas/uso terapêutico , Prognóstico , Pirimidinas/uso terapêutico , Adulto Jovem
8.
PLoS One ; 9(1): e84150, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24416201

RESUMO

Numerous factors impact on the prognosis of acute myeloid leukemia (AML), among which molecular genetic abnormalities are developed increasingly, however, accurate prediction for newly diagnosed AML patients remains unsatisfied. For further improving the prognosis evaluation system, we investigated the transcripts levels of PDCD7, FIS1, FAM3A, CA6, APP, KLRF1, ATCAY, GGT5 and Ang2 in 97 AML patients and 30 non-malignant controls, and validated using the published microarray data from 225 cytogenetically normal AML (CN-AML) patients treated according to the German AMLCG-1999 protocol. Real-time quantitative polymerase chain reaction and western blot were carried out, and clinical data were collected and analyzed. High Ang2 and FIS1 expression discriminated the CR rate of AML patients (62.5% versus 82.9% for Ang2, P = 0.011; 61.4% versus 82.2% for FIS1, P = 0.029). In CN-AML, patients with high FIS1 expression were more likely to be resistant to two courses of induction (P = 0.035). Overall survival (OS) and relapse-free survival (RFS) were shorter in CN-AML patients with high PDCD7 expression (P<0.001; P = 0.006), and PDCD7 was revealed to be an independent risk factor for OS in CN-AML (P = 0.004). In the analysis of published data from 225 CN-AML patients, PDCD7 remained independently predicting OS in CN-AML (P = 0.039). As a conclusion, Ang2 and FIS1 seem related to decreased CR rate of AML patients, and PDCD7 is associated with shorter OS and RFS in CN-AML. Hence, PDCD7, Ang2 and FIS1 may indicate a more aggressive form and poor prognosis of AML.


Assuntos
Proteínas Reguladoras de Apoptose/genética , Biomarcadores Tumorais/metabolismo , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/genética , Proteínas de Membrana/genética , Proteínas Mitocondriais/genética , Fatores de Transcrição/genética , Proteínas de Transporte Vesicular/genética , Adolescente , Adulto , Idoso , Proteínas Reguladoras de Apoptose/metabolismo , Estudos de Casos e Controles , Análise Citogenética , Feminino , Regulação Leucêmica da Expressão Gênica , Humanos , Immunoblotting , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Masculino , Proteínas de Membrana/metabolismo , Pessoa de Meia-Idade , Proteínas Mitocondriais/metabolismo , Análise Multivariada , Análise de Sequência com Séries de Oligonucleotídeos , Prognóstico , Receptores de Células Matadoras Naturais/genética , Receptores de Células Matadoras Naturais/metabolismo , Recidiva , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sobrevida , Fatores de Transcrição/metabolismo , Resultado do Tratamento , Proteínas de Transporte Vesicular/metabolismo , Adulto Jovem
9.
Wei Sheng Yan Jiu ; 43(6): 967-71, 2014 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-25603608

RESUMO

OBJECTIVE: To explore the gene expressions of endoplasmic reticulum stress and differentiation in osteoblast treated by excess fluoride. METHODS: Using primary cultured human osteoblasts for fluorosis model in vitro, apoptosis was inspected by flow cytometer, and RNA was extracted for examination of the unfolded protein response and bone differentiation genes. RESULTS: Fluoride could cause endoplasm reticulum stress in osteoblasts by 15 genes upregulated, 1 gene downregulated. These genes involved PERK, IRE1 and ATF6 signaling pathways of endoplasmic reticulum stress. Meanwhile 32 osteogenesis genes were upregulated, and 2 genes downregulated, involving collagen, matrix metalloproteinase, integrin, bone morphogenetic protein, vascular endothelial growth factor, and tumor necrosis factor gene. CONCLUSION: Excess fluoride can cause endoplasmic stress in osteoblast, while have an impact on the gene expression of osteogenesis.


Assuntos
Estresse do Retículo Endoplasmático/efeitos dos fármacos , Estresse do Retículo Endoplasmático/fisiologia , Fluoretos/farmacologia , Osteoblastos/efeitos dos fármacos , Osteogênese/fisiologia , Resposta a Proteínas não Dobradas/efeitos dos fármacos , Animais , Apoptose , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/genética , Relação Dose-Resposta a Droga , Estresse do Retículo Endoplasmático/genética , Intoxicação por Flúor , Expressão Gênica/efeitos dos fármacos , Humanos , Fosfatos , Transdução de Sinais , Resposta a Proteínas não Dobradas/genética , Fator A de Crescimento do Endotélio Vascular
10.
Microsc Res Tech ; 76(6): 598-605, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23495217

RESUMO

The morphology of the trigeminal ganglion in human fetus was investigated by means of the tract-tracing method using the lipophilic dye DiI-C18-(3) (1,1'-double octadecane 3,3,3'3'-tetramethyl indole carbonyl cyanine-perchlorate), hematoxylin-eosin (HE) stain, and three-dimensional computer reconstruction models. The trigeminal ganglion was flat in the dorsoventral direction, and DiI staining revealed that the trigeminal ganglion cells were somatotopically distributed in the ganglion in a way that reflected the mediolateral order of the three branches. Ganglion cells of the ophthalmic nerve were distributed in the anteromedial part of the trigeminal ganglion, those of the mandibular nerve were in the posterolateral part, and those of the maxillary nerve were localized in the intermediate part. DiI labeled both ganglion cells and nerve fibers in the trigeminal ganglion; the ganglion cells varied in size and appeared as round- or oval-shaped, the neurites connected the cell soma, and some bipolar neurons were also observed. The number of embryonic trigeminal ganglion cells did not significantly change with gestational age, but the cell diameter, area, and perimeter significantly increased. The motor root leaves the pons, runs along the sensory root, passes the ventral surface of the ganglion, and finally runs together with the mandibular nerve. The findings reported here elucidate the morphology, development, and somatotopic organization of the trigeminal ganglion and reveal the trigeminal nerve motor root pathway along the trigeminal ganglion and mandibular nerve in the human fetus.


Assuntos
Feto/anatomia & histologia , Gânglio Trigeminal/anatomia & histologia , Humanos , Imagem Tridimensional , Microscopia , Técnicas de Rastreamento Neuroanatômico , Coloração e Rotulagem
11.
Nan Fang Yi Ke Da Xue Xue Bao ; 32(10): 1457-60, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23076184

RESUMO

OBJECTIVE: To optimize pre-coated multiple-probe fluorescence in situ hybridization (FISH) to improve its efficiency in cytogenetic diagnosis of acute leukemia. METHODS: The original multiple-probe FISH techniques were optimized by adjusting the cell density and adding a process of protease digestion. Cytogenetic anomalies were detected in 141 patients with acute lymphocytic leukemia (ALL) or acute myeloid leukemia/ myelodysplastic syndromes (AML/MDS) using the modified technique, and 35 of the patients were also examined using the original technique. The successful detection rate and positive site detection rate were compared between the modified and original techniques. RESULTS: Modification of the pre-coated multiple-probe FISH technique resulted in an significant increase of the successful detection rate (from 85.3% to 100%) and the positive site detection rate (from 5.1% to 8.6%) in ALL patients; in AML/MDS patients, the successful detection rate was significantly improved from 67.4% to 99.8% and the positive site detection rate from 3.5% to 6.0% (P<0.01). CONCLUSION: The modified pre-coated multiple-probe FISH technique can significantly increase the diagnostic efficiency of cytogenetic abnormalities in leukemic patients.


Assuntos
Hibridização in Situ Fluorescente/métodos , Leucemia Mieloide Aguda/diagnóstico , Síndromes Mielodisplásicas/diagnóstico , Aberrações Cromossômicas , Humanos , Cariotipagem , Leucemia Mieloide Aguda/genética , Síndromes Mielodisplásicas/genética
12.
Nan Fang Yi Ke Da Xue Xue Bao ; 32(5): 707-9, 2012 May.
Artigo em Chinês | MEDLINE | ID: mdl-22588930

RESUMO

OBJECTIVE: To investigate the cytogenetic differences between children and adults with acute lymphoblastic leukemia (ALL) using eight-probe fluorescence in situ hybridization and karyotype analysis. METHODS: Eight-probe (MYC, P16, E2A, TEL/AML1, BCR/ABL , MLL , IGH, and hyperdiploidy) fluorescence in situ hybridization and karyotype analysis were performed for 86 adults and 39 children with acute lymphoblastic leukemia. RESULTS: Eight-probe fluorescence in situ hybridization showed significant differences in the positivity rate of TEL/AML1, BCR/ABL, and hyperdiploidy between adult patients and children with ALL. By karyotype analysis, the positivity rate of t(9;22) and hyperdiploidy differed significantly between the children and adult patients (P<0.05). CONCLUSION: Adults and children with ALL have different expression profiles of the fusion genes. Eight-probe fluorescence in situ hybridization is time-saving, accurate and efficient in detecting common genetic abnormalities in ALL patients, and can be well complementary to karyotype analysis in clinical diagnosis of ALL.


Assuntos
Hibridização in Situ Fluorescente/métodos , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Adolescente , Adulto , Criança , Pré-Escolar , Citogenética , Feminino , Humanos , Lactente , Cariótipo , Cariotipagem , Masculino , Pessoa de Meia-Idade , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Adulto Jovem
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