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1.
Sci Rep ; 11(1): 22796, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34815498

RESUMO

The current severe situation of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has not been reversed and posed great threats to global health. Therefore, there is an urgent need to find out effective antiviral drugs. The 3-chymotrypsin-like protease (3CLpro) in SARS-CoV-2 serve as a promising anti-virus target due to its essential role in the regulation of virus reproduction. Here, we report an improved integrated approach to identify effective 3CLpro inhibitors from effective Chinese herbal formulas. With this approach, we identified the 5 natural products (NPs) including narcissoside, kaempferol-3-O-gentiobioside, rutin, vicenin-2 and isoschaftoside as potential anti-SARS-CoV-2 candidates. Subsequent molecular dynamics simulation additionally revealed that these molecules can be tightly bound to 3CLpro and confirmed effectiveness against COVID-19. Moreover, kaempferol-3-o-gentiobioside, vicenin-2 and isoschaftoside were first reported to have SARS-CoV-2 3CLpro inhibitory activity. In summary, this optimized integrated strategy for drug screening can be utilized in the discovery of antiviral drugs to achieve rapid acquisition of drugs with specific effects on antiviral targets.

2.
Mol Carcinog ; 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34622496

RESUMO

Enhancer RNAs (eRNAs) are a subclass of long noncoding RNAs (lncRNAs) that have a wide effect in human tumors. However, the systematic analysis of potential functions of eRNAs-related genes (eRGs) in colon cancer (CC) remains unexplored. In this study, a total of 8231 eRGs including 6236 protein-coding genes and 1995 lncRNAs were identified in CC based on the multiple resources. These eRGs showed higher expression level and stability compared to other genes. What's more, the functions of these eRGs were closely related to cancer. Then a prognostic prediction model with 12 eRGs signatures were obtained for colon adenocarcinoma (COAD) patients. ROC curves showed the AUCs were 0.81, 0.77, and 0.78 for 1-, 3-, and 5-year survival prediction, respectively. And the prognostic model also manifested good performance in the validation datasets. Besides, the expression levels of two prognostic signatures, TMEM220 and LRRN2, were verified to be significantly lower in CC tissues than in adjacent noncancerous tissues (p < .05). Finally, the distinct molecular features were characterized between the high- and low-risk group through multiomics analysis including DNA mutation and methylation. Our results show eRGs signatures based prognostic model has high accuracy and may provide innovative biomarkers in COAD.

3.
Environ Pollut ; 289: 117830, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34325095

RESUMO

Development of efficient, green and low-cost natural mineral-based reductive materials is promising to remediation of hexavalent chromium(Cr(VI))-contaminated soil. Considering the synergetic effect between pyrite and zerovalent iron (ZVI), an activated pyrite supported ZVI(ZVI/FeS2) with high reducing activity was developed by ball milling activation of natural pyrite and sulfidation of ZVI. The remediation property of ZVI/FeS2 for Cr(VI)-contaminated soil was evaluated with different ZVI/FeS2 dosage, soil-water ratio, initial pH, time and temperature, as well as the stability of Cr. The results showed that ZVI/FeS2 possessed high reduction activity with soil Cr(VI) removal rate up to 99 % even under alkaline condition, and soil with different pH values eventually converged to neutral after 90 days, indicating that ZVI/FeS2 has a good self-regulating alkaline ability. The reduction process conformed to Langmuir-Hinshelwood first-order kinetics and was a spontaneous and endothermic process. The lower activation energy of 17.97 kJ mol-1 (usually 60-250 kJ mol-1) indicated that the reduction reaction of Cr(VI) was particularly easy to occur. The speciation change of Cr in soil within 30 days demonstrated that the Cr in the soil was converted from a readily migratable state to a more stable state, where the Fe-Mn oxide bound fraction reached 85.03 % due to the generation of Cr(III)/Fe(III) co-precipitation. The results of long-term stability experiments showed that the leaching concentrations of Cr(VI) and total Cr decreased significantly after the ZVI/FeS2 treatment and remained stable at very low levels for 180 days. This study provided a sustainable way to fully utilize natural pyrite minerals to obtain iron-bearing reductive materials for feasible, effective and long-term stable immobilization of Cr(VI) in soil.


Assuntos
Poluentes do Solo , Cromo/análise , Compostos Férricos , Ferro , Cinética , Solo , Poluentes do Solo/análise , Sulfetos , Termodinâmica
4.
Sci Rep ; 11(1): 14508, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-34267294

RESUMO

For determine the optimum position of the roof low roadway, the optimal solution is derived according to the response surface methodology. The UDEC numerical simulation of the overburden gives the porosity distribution of the strike fractured zone, the upper limit heights of the caving zone and the fractured zone are obtained as 18 m and 65 m, respectively. Based on the porosity distribution, the FLUENT numerical models of the goaf zone, air inlet roadway, air return roadway, working face and roof low roadway were established to simulate the gas concentration in the upper corner and gas drainage volume in roof low roadway during mining. Using the vertical and horizontal distance of the roof low roadway as the influencing factors, the experimental scheme of the position of the roof low roadway was designed according to the response surface method, and the response values were obtained from the FLUENT simulation experiments, predicting that the vertical and horizontal distances of the roof low roadway were 7.7 m and 5.9 m respectively when the interaction between the gas concentration in the upper corner and gas drainage volume in roof low roadway was optimal. Field tests showed that the average gas concentration in the upper corner and the average gas drainage volume in roof low roadway were 0.432% and 40.861 m3/min respectively, both of which were less than 10% of the error from the simulations. The design of the roof low roadway has effectively managed the gas accumulation problem in the upper corner.

5.
Front Oncol ; 11: 685515, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34211853

RESUMO

Left-sided colon cancer (LCC) and right-sided colon cancer (RCC) have distinct characteristics in tumor immune microenvironment (TIME). Although existing studies have shown a strong association between gene mutations and TIME, whether the regulatory mechanisms between gene mutations and TIME are different between RCC and LCC is still unclear. In this study, we showed the fractions of CD8+ T cells were higher while those of regulatory T cells were lower in RCC. Besides, a stronger association between gene mutations and TIME was observed in RCC. Specifically, using multi-omics data, we demonstrated the mutations of most top mutated genes (TMGs) including BRAF, PCLO, MUC16, LRP2, ANK3, KMT2D, RYR2 made great contributions to elevated fraction of immune cells by up-regulating immune-related genes directly or indirectly through miRNA and DNA methylation, whereas the effects of APC, TP53 and KRAS mutations on TIME were reversed in RCC. Remarkably, we found the expression levels of several immune checkpoint molecules such as PD-1 and LAG3 were correlated with corresponding DNA methylation levels, which were associated with the mutations of TMGs in RCC. In contrast, the associations between gene mutations and TIME were less significant in LCC. Besides, survival analyses showed APC mutation had adverse impact on immunotherapy while patients with BRAF mutation were more suitable for immunotherapy in colon cancer. We hope that our results will provide a deeper insight into the sophisticated mechanism underlying the regulation between mutations and TIME, and thus boost the discovery of differential immunotherapeutic strategies for RCC and LCC.

6.
Infect Genet Evol ; 93: 104992, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34242773

RESUMO

Enterovirus D68 (EV-D68), as one of the major pathogens of paediatric respiratory disease, has been widely spread in the population in recent years. As the basis of virus antigenicity, antigenic epitopes are essential to monitoring the transformation of virus antigenicity. However, there is a lack of systematic studies on the antigenic epitopes of EV-D68. In this study, a bioinformatics-based prediction algorithm for human enteroviruses was used to predict the conformational epitopes of EV-D68. The prediction results showed that the conformational epitopes of EV-D68 were clustered into three sites: site 1, site 2, and site 3. Site 1 was located in the "north rim" region of the canyon near the fivefold axis; site 2 was located in the "puff" region near the twofold axis; and site 3 consisted of two parts, one in the "knob" region on the south rim of the canyon and the other in the threefold axis region. The predicted epitopes overlapped highly with the binding regions of four reported monoclonal antibodies (mAbs), indicating that the predictions were highly reliable. Phylogenetic analysis showed that amino acid mutations in the epitopes of the VP1 BC loop, DE loop, C-terminus, and VP2 EF loop played a crucial role in the evolutionary divergence of EV-D68 clades/subclades and epidemics. This finding indicated that the VP1 BC loop, DE loop, C-terminus, and VP2 EF loop were the most important epitopes of EV-D68. Research on the epitopes of EV-D68 will contribute to outbreak surveillance and to the development of diagnostic reagents and recombinant vaccines.

7.
Chemosphere ; 281: 130957, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34289619

RESUMO

It is a challenge to simultaneously treat the combined pollutants of chromium(VI) (Cr(VI)) and organics (such as phenol) in wastewater. Here, a stable and efficient redox system based on FeS2 sulfidated zero valent iron (FeS2/Fe0) and persulfate (PS) was developed to synchronously remove Cr(VI) and phenol. 100% of phenol (10 mg/L) was oxidized in 10 min and Cr(VI) (20 mg/L) was completely reduced to Cr(III) in 90 min in the FeS2/Fe0+PS system with a pH range of 3.0-9.0, respectively. phenol was selectively oxidized without re-oxidizing Cr(III) in such system. The surface-bound Fe2+ was the major reactive species to synchronously reduce Cr(VI) and oxidize phenol. The mechanisms were elucidated that the phenol degradation was accelerated by the generated Cr(III) complexing with its products, and that SO42-, whose production speed was accelerated by the PS activation to oxidize phenol and FeS2, was conductive to corrode Fe0 to regenerate the surface-bound Fe2+ for reducing Cr(VI) and oxidizing phenol. It is potential to develop a high-performance and large-scaled FeS2/Fe0-based redox platform to remediate the complex pollution of Cr(VI) and organics.


Assuntos
Cromo , Poluentes Químicos da Água , Cromo/análise , Oxirredução , Estresse Oxidativo , Fenóis , Poluentes Químicos da Água/análise
8.
Gynecol Endocrinol ; 37(7): 665-671, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34126831

RESUMO

OBJECTIVES: MicroRNAs (miRNAs) are potentially involved in the regulation of glucose and lipid metabolism. The aim of this study was to investigate potential miRNA regulators for serum lipids and blood glucose in gestational diabetes mellitus. METHODS: Plasma samples were obtained from 53 women with GDM and 46 normal pregnant women. Fasting blood glucose and a blood lipid profile were measured. Plasma miRNA expression profiles were analyzed using microarray. To verify the microarray data, the expression of miRNAs was evaluated by real-time PCR. Gene ontology (GO) and genes and genomics (KEGG) pathway enrichment of the predicted target genes of miRNAs were analyzed. RESULTS: The miRNA expression profiles of plasma samples from healthy and GDM women are distinct. We identified 93 differently expressed miRNAs. Compared with healthy pregnant women, 48 miRNAs including miR-574-5p and miR-3135b exhibited significantly lower expression in plasma samples from GDM patients. The expression of miR-574-5p was significantly correlated with levels of blood glucose and LDL-C; miR-3135b was significantly correlated with HDL-C. Some predicted common target genes of these two miRNAs are associated with the metabolism of glucose and lipids as well as the insulin signaling pathway. CONCLUSIONS: miR-574-5p and miR-3135b may serve as metabolic regulators of glucose and lipids for GDM.

9.
Nucleic Acids Res ; 49(W1): W459-W468, 2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-34050762

RESUMO

Increasing evidence proves the essential regulatory roles of non-coding RNAs (ncRNAs) in biological processes. However, characterizing the specific functions of ncRNAs remains a challenging task, owing to the intensive consumption of the experimental approaches. Here, we present an online platform ncFANs v2.0 that is a significantly enhanced version of our previous ncFANs to provide multiple computational methods for ncRNA functional annotation. Specifically, ncFANs v2.0 was updated to embed three functional modules, including ncFANs-NET, ncFANs-eLnc and ncFANs-CHIP. ncFANs-NET is a new module designed for data-free functional annotation based on four kinds of pre-built networks, including the co-expression network, co-methylation network, long non-coding RNA (lncRNA)-centric regulatory network and random forest-based network. ncFANs-eLnc enables the one-stop identification of enhancer-derived lncRNAs from the de novo assembled transcriptome based on the user-defined or our pre-annotated enhancers. Moreover, ncFANs-CHIP inherits the original functions for microarray data-based functional annotation and supports more chip types. We believe that our ncFANs v2.0 carries sufficient convenience and practicability for biological researchers and facilitates unraveling the regulatory mechanisms of ncRNAs. The ncFANs v2.0 server is freely available at http://bioinfo.org/ncfans or http://ncfans.gene.ac.


Assuntos
RNA não Traduzido/metabolismo , Software , Elementos Facilitadores Genéticos , Humanos , Metilação , Anotação de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo
10.
PLoS One ; 16(4): e0247423, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33793559

RESUMO

Human parechoviruses (HPeVs) are human pathogens that usually cause diseases ranging from rash to neonatal sepsis in young children. HPeV1 and HPeV3 are the most frequently reported genotypes and their three-dimensional structures have been determined. However, there is a lack of systematic research on the antigenic epitopes of HPeVs, which are useful for understanding virus-receptor interactions, developing antiviral agents or molecular diagnostic tools, and monitoring antigenic evolution. Thus, we systematically predicted and compared the conformational epitopes of HPeV1 and HPeV3 using bioinformatics methods in the study. The results showed that both epitopes clustered into three sites (sites 1, 2 and 3). Site 1 was located on the "northern rim" near the fivefold vertex; site 2 was on the "puff"; and site 3 was divided into two parts, of which one was located on the "knob" and the other was close to the threefold vertex. The predicted epitopes highly overlapped with the reported antigenic epitopes, which indicated that the prediction results were accurate. Although the distribution positions of the epitopes of HPeV1 and HPeV3 were highly consistent, the residues varied largely and determined the genotypes. Three amino acid residues, VP3-91N, -92H and VP0-257S, were the key residues for monoclonal antibody (mAb) AM28 binding to HPeV1 and were also of great significance in distinguishing HPeV1 and HPeV3. We also found that two residues, VP1-85N and -87D, might affect the capability of mAb AT12-015 to bind to HPeV3.


Assuntos
Epitopos/imunologia , Parechovirus/imunologia , Infecções por Picornaviridae/virologia , Anticorpos Monoclonais/química , Anticorpos Monoclonais/imunologia , Anticorpos Neutralizantes/química , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/química , Anticorpos Antivirais/imunologia , Pré-Escolar , Biologia Computacional/métodos , Epitopos/química , Humanos , Parechovirus/química , Conformação Proteica
11.
Int J Mol Sci ; 22(6)2021 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-33804745

RESUMO

Endothelial autocrine signaling is essential to maintain vascular homeostasis. There is limited information about the role of endothelial autocrine signaling in regulating severe pulmonary vascular remodeling during the onset of pulmonary arterial hypertension (PAH). In this study, we employed the first severe pulmonary hypertension (PH) mouse model, Egln1Tie2Cre (Tie2Cre-mediated disruption of Egln1) mice, to identify the novel autocrine signaling mediating the pulmonary vascular endothelial cell (PVEC) proliferation and the pathogenesis of PAH. PVECs isolated from Egln1Tie2Cre lung expressed upregulation of many growth factors or angiocrine factors such as CXCL12, and exhibited pro-proliferative phenotype coincident with the upregulation of proliferation-specific transcriptional factor FoxM1. Treatment of CXCL12 on PVECs increased FoxM1 expression, which was blocked by CXCL12 receptor CXCR4 antagonist AMD3100 in cultured human PVECs. The endothelial specific deletion of Cxcl12(Egln1/Cxcl12Tie2Cre) or AMD3100 treatment in Egln1Tie2Cre mice downregulated FoxM1 expression in vivo. We then generated and characterized a novel mouse model with endothelial specific FoxM1 deletion in Egln1Tie2Cre mice (Egln1/Foxm1Tie2Cre), and found that endothelial FoxM1 deletion reduced pulmonary vascular remodeling and right ventricular systolic pressure. Together, our study identified a novel mechanism of endothelial autocrine signaling in regulating PVEC proliferation and pulmonary vascular remodeling in PAH.


Assuntos
Comunicação Autócrina , Quimiocina CXCL12/metabolismo , Células Endoteliais/metabolismo , Proteína Forkhead Box M1/metabolismo , Hipertensão Arterial Pulmonar/etiologia , Hipertensão Arterial Pulmonar/metabolismo , Receptores CXCR4/metabolismo , Transdução de Sinais , Animais , Biomarcadores , Células Cultivadas , Modelos Animais de Doenças , Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Imunofluorescência , Humanos , Hipóxia/metabolismo , Imuno-Histoquímica , Camundongos , Camundongos Transgênicos , Hipertensão Arterial Pulmonar/diagnóstico , Índice de Gravidade de Doença , Remodelação Vascular
12.
Opt Express ; 29(6): 9030-9042, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33820341

RESUMO

This work theoretically investigates the frequency noise and spectral linewidth characteristics of mutually delay-coupled quantum cascade lasers, which are operated in the stable locking regime. We demonstrate that the mutual injection significantly reduces the frequency noise at proper coupling phases. However, the relative intensity noise is insensitive to the mutual injection. Influences of the pump current, the linewidth broadening factor, the coupling phase, and the delay time on the frequency noise are discussed as well. In addition, it is found that the appearance of multiple compound laser modes can deteriorate the frequency noise performance of the lasers.

13.
Sci Total Environ ; 783: 146906, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-33866177

RESUMO

As climate issues gradually attract public attention worldwide, the operation and construction of oil product pipelines have been attached with new energy-saving and emission-reduction targets. Though previous studies concerning Life Cycle Assessment of oil and gas pipelines have estimated the carbon footprint to some extent, there is a lack of researches that take the characteristics of oil products pipelines into consideration. Oil products pipelines undertake the task of delivering various products to downstream demand locations, which differs greatly from other pipeline transportation systems as back-to-back sequential delivery is adopted. In this paper, a detailed Life Cycle Assessment model is established to analyze carbon emissions of oil products pipeline system from construction to disposal as well as its impact on soil environment. Data from practical pipes is adopted as the case study to reflect emissions produced in different stages, and the amount of total and unified emissions of different pipes provided through the proposed model is within the range of 2.78 to 4.70 tCO2e/t·km. Then, sensitivity analysis is carried out to identify the driving factors of emissions. According to the calculation results, pipe length, diameter and throughput turn out to be the dominating factors, and an empirical formula is derived for future planned pipes. Relevant recommendations are put forward based on the results to help reduce emissions from oil product pipe transportation.

14.
Biochem Biophys Res Commun ; 554: 25-32, 2021 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-33774276

RESUMO

Osteosarcoma, a highly aggressive malignant tumor of the bone, usually occurs in children and young adults. However, although the considerable achievement in the clinical treatment of osteosarcoma recent years, the overall survival of osteosarcoma patients has not been obviously improved. Cancer cells preferentially use glycolysis instead of oxidative phosphorylation to meet their increased energetic and biosynthetic demands, a phenomenon known as the Warburg effect. Glycolysis is a driving factor in multiple cancers and is emerging as a new cancer target treatment. In the present study, we established a model to screen for glycolysis-associated genes in osteosarcoma. This risk score of the model were correlated with clinical characteristics osteosarcoma patients. Besides, a functional assay identified that STC2 enhanced the glycolysis of osteosarcoma cells. Modulation of STC2 changes glucose consumption and lactate production as well as GLUT1 expression in osteosarcoma. Furthermore, we identified that change in the expression levels of STC2 affected the proliferation, invasion, and migration of osteosarcoma cells. Our findings showed STC2 as a new tumor-promoting factor of osteosarcoma cells through enhancing glycolysis.


Assuntos
Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/patologia , Glucose/metabolismo , Glicoproteínas/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Ácido Láctico/metabolismo , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Neoplasias Ósseas/genética , Linhagem Celular Tumoral , Movimento Celular/fisiologia , Proliferação de Células/fisiologia , Biologia Computacional , Bases de Dados Genéticas , Glicólise , Glicoproteínas/genética , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Osteossarcoma/genética , Prognóstico , Taxa de Sobrevida
15.
Sci Rep ; 11(1): 5701, 2021 03 11.
Artigo em Inglês | MEDLINE | ID: mdl-33707530

RESUMO

Enterovirus A71 (EV-A71), Coxsackievirus A16 (CV-A16) and CV-A10 are the major causative agents of hand, foot and mouth disease (HFMD). The conformational epitopes play a vital role in monitoring the antigenic evolution, predicting dominant strains and preparing vaccines. In this study, we employed a Bioinformatics-based algorithm to predict the conformational epitopes of EV-A71 and CV-A16 and compared with that of CV-A10. Prediction results revealed that the distribution patterns of conformational epitopes of EV-A71 and CV-A16 were similar to that of CV-A10 and their epitopes likewise consisted of three sites: site 1 (on the "north rim" of the canyon around the fivefold vertex), site 2 (on the "puff") and site 3 (one part was in the "knob" and the other was near the threefold vertex). The reported epitopes highly overlapped with our predicted epitopes indicating the predicted results were reliable. These data suggested that three-site distribution pattern may be the basic distribution role of epitopes on the enteroviruses capsids. Our prediction results of EV-A71 and CV-A16 can provide essential information for monitoring the antigenic evolution of enterovirus.

16.
J Environ Sci (China) ; 103: 20-32, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33743902

RESUMO

The region along the Taihang Mountains in the North China Plain (NCP) is characterized by serious fine particle pollution. To clarify the formation mechanism and controlling factors, an observational study was conducted to investigate the physical and chemical properties of the fine particulate matter in Jiaozuo city, China. Mass concentrations of the water-soluble ions (WSIs) in PM2.5 and gaseous pollutant precursors were measured on an hourly basis from December 1, 2017, to February 27, 2018. The positive matrix factorization (PMF) method and the FLEXible PARTicle (FLEXPART) model were employed to identify the sources of PM2.5. The results showed that the average mass concentration of PM2.5 was 111 µg/m3 during the observation period. Among the major WSIs, sulfate, nitrate, and ammonium (SNA) constituted 62% of the total PM2.5 mass, and NO3- ranked the highest with an average contribution of 24.6%. NH4+ was abundant in most cases in Jiaozuo. According to chemical balance analysis, SO42-, NO3-, and Cl- might be present in the form of (NH4)2SO4, NH4NO3, NH4Cl, and KCl. The liquid-phase oxidation of SO2 and NO2 was severe during the haze period. The relative humidity and pH were the key factors influencing SO42- formation. We found that NO3- mainly stemmed from homogeneous gas-phase reactions in the daytime and originated from the hydrolysis of N2O5 in the nighttime, which was inconsistent with previous studies. The PMF model identified five sources of PM2.5: secondary origin (37.8%), vehicular emissions (34.7%), biomass burning (11.5%), coal combustion (9.4%), and crustal dust (6.6%).


Assuntos
Poluentes Atmosféricos , Aerossóis/análise , Poluentes Atmosféricos/análise , China , Cidades , Monitoramento Ambiental , Material Particulado/análise , Estações do Ano , Emissões de Veículos/análise
17.
Appl Energy ; 283: 116339, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33753961

RESUMO

With the coronavirus pandemic wreathing havoc around the world, power industry has been hit hard due to the proposal of lockdown policies. However, the impact of lockdowns and shutdowns on the power system in different regions as well as periods of the pandemic can hardly be reflected on the foundation of current studies. In this paper, a prediction-based analysis method is developed to point out the electricity consumption gap resulted from the pandemic situation. The core of this method is a novel optimized grey prediction model, namely Rolling IMSGM(1,1) (Rolling Mechanism combined with grey model with initial condition as Maclaurin series), which achieves better prediction results in the face of long-term emergencies. A novel initial condition is adopted to track data with various characteristics in the form of higher-order polynomials, which are then determined by intelligent algorithms to realize accurate fitting. Historical power consumption data in China are utilized to carry out the monthly forecasts during COVID-19. Compared with other competitive models' prediction results, the superiority of IMSGM(1,1) are demonstrated. Through analyzing the gap between predicted consumption values and the actual data, it can be found that the impact of the pandemic on electricity varies in different periods, which is related to its severity and the local lockdown policies. This study helps to understand the impact on power industry in the face of such an emergency intuitively so as to respond to possible future events.

18.
J Cell Mol Med ; 25(7): 3300-3311, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33611811

RESUMO

Ferroptosis is an iron-dependent mode of non-apoptotic cell death characterized by accumulation of lipid reactive oxygen species (ROS). As a regulator of ROS, cytoglobin (CYGB) plays an important role in oxygen homeostasis and acts as a tumour suppressor. However, the mechanism by which CYGB regulates cell death is largely unknown. Here, we show that CYGB overexpression increased ROS accumulation and disrupted mitochondrial function as determined by the oxygen consumption rate and membrane potential. Importantly, ferroptotic features with accumulated lipid ROS and malondialdehyde were observed in CYGB-overexpressing colorectal cancer cells. Moreover, CYGB significantly increased the sensitivity of cancer cells to RSL3- and erastin-induced ferroptotic cell death. Mechanically, both YAP1 and p53 were significantly increased based on the RNA sequencing. The knock-down of YAP1 alleviated production of lipid ROS and sensitivity to ferroptosis in CYGB overexpressed cells. Furthermore, YAP1 was identified to be inhibited by p53 knock-down. Finally, high expression level of CYGB had the close correlation with key genes YAP1 and ACSL4 in ferroptosis pathway in colon cancer based on analysis from TCGA data. Collectively, our results demonstrated a novel tumour suppressor role of CYGB through p53-YAP1 axis in regulating ferroptosis and suggested a potential therapeutic approach for colon cancer.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Neoplasias do Colo/metabolismo , Citoglobina/genética , Ferroptose , Fatores de Transcrição/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Carbolinas/toxicidade , Neoplasias do Colo/genética , Citoglobina/metabolismo , Células HCT116 , Humanos , Piperazinas/toxicidade , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Regulação para Cima
19.
Environ Technol ; : 1-8, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33577396

RESUMO

A previous isolated Gordonia sp. (Lff) was used to degrade di-n-octyl phthalate (DOP) contamination in both aqueous solution and soil. The influence of temperature, pH, inoculum size, salt content and initial concentration of DOP on DOP degradation by Lff were analysed. The response of soil bacterial community to DOP and Lff was also analysed by Illumina MiSeq sequence method. Results showed that the optimal temperature, pH, inoculum size and salt content were 35oC, 8.0, 5% and <5%, respectively. Under the optimal condition, more than 91.25% of DOP with different initial concentrations (100-2000 mg/L) could be degraded by Lff. Kinetics analysis indicated that biodegradation of DOP by Lff could be described by first-order kinetics (R2 > 0.917) with the half-life (t1/2) changing irregularly between 0.58 and 0.83 d. In addition, Lff enhanced the removal of DOP in soil and alleviated the toxicity of DOP on soil microorganisms. Furthermore, its influence on soil bacterial community is not obvious. These results suggested that Lff was effective in remediating DOP contamination in different environments.

20.
Sci Total Environ ; 773: 145654, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33582355

RESUMO

Chemical reduction of Cr(VI) to Cr(III) by reductive materials is the most widely used technology for the remediation of Cr(VI)-contaminated soil due to its high efficiency, adaptability and low cost. This paper reviews chromium chemistry and the materials that can effectively reduce Cr(VI) to Cr(III) for the remediation of Cr(VI)-contaminated soil, namely iron-bearing reductants, sulfur-based compounds and organic amendments. Moreover, we discuss the corresponding mechanisms involved in the process of immobilization of Cr(VI) in polluted soil, and emphasize the relationship between the materials remediation performance and soil environmental conditions. Besides, perspectives on the potential future researches of novel materials design and technological development in the remediation of Cr(VI) contaminated soil are also put forward.

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