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1.
Emerg Infect Dis ; 26(1): 164-166, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31855545

RESUMO

We investigated the epidemiology of cholera in Taiwan during 2002-2018. Vibrio cholerae sequence type (ST) 75 clone emerged in 2009 and has since become more prevalent than the ST69 clone from a previous pandemic. Closely related ST75 strains have emerged in 4 countries and may now be widespread in Asia.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31383653

RESUMO

Salmonella enterica serovar Goldcoast infection was rare in Taiwan; it was not detected in routine surveillance from 2004 to 2013. This serovar was first identified in 2014, but the frequency of infection remained low until 2017. From 2014 to 2016, all but one isolate was pan-susceptible. S Goldcoast infections abruptly increased in 2018, and all isolates were multidrug-resistant (MDR). All MDR isolates harbored an IncHI2 plasmid, and the majority carried 14 antimicrobial resistance genes, aac(3)-IId, aadA22, aph(3')-Ia, aph(6)-Id, bla TEM-1B, bla CTX-M-55, lnu(F), floR, qnrS13, arr-2, sul2, sul3, tet(A), and dfrA14. S Goldcoast strains recovered in Taiwan and 96 of 99 strains from Germany, the Netherlands, the United Kingdom, and the United States belonged to sequence type 358 (ST358). Whole-genome single-nucleotide polymorphism and core genome multilocus sequence type analyses revealed that all strains of the ST358 clone shared a high degree of genetic relatedness. The present study highlighted that a dramatic increase in S Goldcoast infections followed the emergence of MDR strains and indicated that a genetically closely related S Goldcoast ST358 clone may have widespread significance internationally.

3.
Emerg Infect Dis ; 25(1): 144-147, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30561315

RESUMO

In 2011, a Salmonella enterica serovar Anatum clone emerged in Taiwan. During 2016-2017, infections increased dramatically, strongly associated with emergence and spread of multidrug-resistant strains with a plasmid carrying 11 resistance genes, including blaDHA-1. Because these resistant strains infect humans and food animals, control measures are urgently needed.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Plasmídeos/genética , Infecções por Salmonella/microbiologia , Salmonella enterica/genética , Humanos , Epidemiologia Molecular , Infecções por Salmonella/epidemiologia , Salmonella enterica/efeitos dos fármacos , Taiwan/epidemiologia
4.
Artigo em Inglês | MEDLINE | ID: mdl-29581119

RESUMO

We identified 20 to 22 resistance genes, carried in four incompatibility groups of plasmids, in each of five genetically closely related Salmonella enterica serovar Typhimurium strains recovered from humans, pigs, and chickens. The genes conferred resistance to aminoglycosides, chloramphenicol, sulfonamides, trimethoprim, tetracycline, fluoroquinolones, extended-spectrum cephalosporins and cefoxitin, and azithromycin. This study demonstrates the transmission of multidrug-resistant Salmonella strains among humans and food animals and may be the first identification of mphA in azithromycin-resistant Salmonella strains in Taiwan.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Plasmídeos/genética , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética , Animais , Azitromicina/farmacologia , Galinhas , Humanos , Testes de Sensibilidade Microbiana , Família Multigênica/genética , Salmonelose Animal , Salmonella typhimurium/isolamento & purificação , Suínos , Taiwan , Sequenciamento Completo do Genoma
5.
Artigo em Inglês | MEDLINE | ID: mdl-28416545

RESUMO

We detected the colistin resistance gene mcr-1 in four Salmonella serovars isolated from humans and animals with diarrhea. The resistance gene was carried on different plasmids. One mcr-1-carrying conjugative plasmid, a variant of pHNSHP45, was disseminated among Salmonella isolates recovered from humans, pigs, and chickens.


Assuntos
Antibacterianos/farmacologia , Plasmídeos/genética , Salmonella/efeitos dos fármacos , Animais , Galinhas , Diarreia/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Suínos
6.
Emerg Infect Dis ; 23(2): 345-346, 2016 02.
Artigo em Inglês | MEDLINE | ID: mdl-28098533

RESUMO

We report an outbreak of azithromycin-nonsusceptible Shigella flexneri 3a infection in Taiwan associated with men who have sex with men. The bacterial strains belonged to the sublineage A of a recently reported outbreak lineage associated with men who have sex with men, characterized by reduced azithromycin susceptibility and circulation in shigellosis low-risk regions.


Assuntos
Antibacterianos/farmacologia , Azitromicina/farmacologia , Disenteria Bacilar/epidemiologia , Homossexualidade Masculina , Doenças Sexualmente Transmissíveis/epidemiologia , Shigella flexneri/isolamento & purificação , Adulto , Surtos de Doenças , Farmacorresistência Bacteriana , Disenteria Bacilar/microbiologia , Disenteria Bacilar/prevenção & controle , Humanos , Masculino , Doenças Sexualmente Transmissíveis/microbiologia , Doenças Sexualmente Transmissíveis/prevenção & controle , Shigella flexneri/efeitos dos fármacos , Shigella flexneri/genética , Taiwan/epidemiologia , Adulto Jovem
7.
Int J Food Microbiol ; 214: 1-3, 2015 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-26208096

RESUMO

We created a database consisting of a large number of Salmonella pulsed-field gel electrophoresis (PFGE) profiles covering a wide range of different serovars. This database was used for the prediction of the serovars based on the PFGE profiles for isolates from Taiwan and Denmark. The PFGE profiles proved very useful in the determination of a serovar although serovar prediction was more efficient for local isolates than those from a distant geographic area. To use a highly stringent band matching tolerance in the BioNumerics software is also important for the grouping of serovars.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Técnicas de Tipagem Bacteriana/normas , Eletroforese em Gel de Campo Pulsado , Salmonella/classificação , Salmonella/genética , Dinamarca , Sorogrupo , Taiwan
8.
PLoS One ; 9(4): e95772, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24760078

RESUMO

We collected 110 Salmonella enterica isolates from sick pigs and determined their serotypes, genotypes using pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility to 12 antimicrobials and compared the data with a collection of 18,280 isolates obtained from humans. The pig isolates fell into 12 common serovars for human salmonellosis in Taiwan; S. Typhimurium, S. Choleraesuis, S. Derby, S. Livingstone, and S. Schwarzengrund were the 5 most common serovars and accounted for a total of 84% of the collection. Of the 110 isolates, 106 (96%) were multidrug resistant (MDR) and 48 (44%) had PFGE patterns found in human isolates. S. Typhimurium, S. Choleraesuis, and S. Schwarzengrund were among the most highly resistant serovars. The majority of the 3 serovars were resistant to 8-11 of the tested antimicrobials. The isolates from pigs and humans sharing a common PFGE pattern displayed identical or very similar resistance patterns and Salmonella strains that caused severe infection in pigs were also capable of causing infections in humans. The results indicate that pigs are one of the major reservoirs to human salmonellosis in Taiwan. Almost all of the pig isolates were MDR, which highlights the necessity of strictly regulating the use of antimicrobials in the agriculture sector in Taiwan.


Assuntos
Salmonella/efeitos dos fármacos , Animais , Anti-Infecciosos/farmacologia , Farmacorresistência Bacteriana Múltipla , Eletroforese em Gel de Campo Pulsado , Humanos , Testes de Sensibilidade Microbiana , Suínos , Taiwan
9.
PLoS One ; 9(1): e85354, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24454847

RESUMO

Infections caused by enterohemorrhagic Escherichia coli (EHEC) can lead to diarrhea with abdominal cramps and sometimes are complicated by severe hemolytic uremic syndrome. EHEC secretes effector proteins into host cells through a type III secretion system that is composed of proteins encoded by a chromosomal island, locus for the enterocyte effacement (LEE). EspA is the major component of the filamentous structure connecting the bacteria and the host's cells. Synthesis and secretion of EspA must be carefully controlled since the protein is prone to polymerize. CesAB, CesA2, and EscL have been identified as being able to interact with EspA. Furthermore, the intracellular level of EspA declines when cesAB, cesA2, and escL are individually deleted. Here, we report a LEE gene named l0033, which also affects the intracellular level of EspA. We renamed l0033 as escA since its counterpart in enteropathogenic E. coli has been recently described. Similar to CesAB, EscL, and CesA2, EscA interacts with EspA and enhances the protein stability of EspA. However, EscA is also able to interact with inner membrane-associated EscL, CesA2, and EscN, but not with cytoplasmic CesAB. In terms of gene organizations, escA locates in LEE3. Expression of EscA is faithfully regulated via Mpc, the first gene product of LEE3. Since Mpc is tightly regulated to low level, we suggest that EscA is highly synchronized and critical to the process of escorting EspA to its final destination.


Assuntos
Escherichia coli O157/metabolismo , Proteínas de Escherichia coli/metabolismo , Mapas de Interação de Proteínas , Sistemas de Secreção Bacterianos , Cromatografia de Afinidade , Deleção de Genes , Modelos Biológicos , Ligação Proteica , Estabilidade Proteica
10.
Foodborne Pathog Dis ; 11(3): 230-3, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24313786

RESUMO

Food sold over the internet is an emerging business that also presents a concern with regard to food safety. A nationwide foodborne disease outbreak associated with sandwiches purchased from an online shop in July 2010 is reported. Consumers were telephone interviewed with a structured questionnaire and specimens were collected for etiological examination. A total of 886 consumers were successfully contacted and completed the questionnaires; 36.6% had become ill, with a median incubation period of 18 h (range, 6-66 h). The major symptoms included diarrhea (89.2%), abdominal pain (69.8%), fever (47.5%), headache (32.7%), and vomiting (17.3%). Microbiological laboratories isolated Salmonella enterica serovar Enteritidis, Salmonella Virchow, Staphylococcus aureus, Bacillus cereus, and enterotoxigenic Escherichia coli from the contaminated sandwiches, Salmonella Enteritidis and Salmonella Virchow from the patients, and Salmonella Enteritidis and Staphylococcus aureus from food handlers. Pulsed-field gel electrophoresis genotyping suggested a common origin of Salmonella bacteria recovered from the patients, food, and a food handler. Among the pathogens detected, the symptoms and incubation period indicated that Salmonella, likely of egg origin, was the probable causative agent of the outbreak. This outbreak illustrates the importance of meticulous hygiene practices during food preparation and temperature control during food shipment and the food safety challenges posed by online food-shopping services.


Assuntos
Surtos de Doenças , Ovos/microbiologia , Microbiologia de Alimentos , Serviços de Alimentação , Intoxicação Alimentar por Salmonella/epidemiologia , Salmonella/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Bacillus cereus/genética , Bacillus cereus/isolamento & purificação , Criança , Pré-Escolar , Escherichia coli Enterotoxigênica/genética , Escherichia coli Enterotoxigênica/isolamento & purificação , Feminino , Serviços de Alimentação/normas , Serviços de Alimentação/tendências , Doenças Transmitidas por Alimentos/epidemiologia , Doenças Transmitidas por Alimentos/microbiologia , Genótipo , Humanos , Lactente , Internet , Masculino , Pessoa de Meia-Idade , Salmonella/genética , Intoxicação Alimentar por Salmonella/microbiologia , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificação , Taiwan/epidemiologia , Adulto Jovem
11.
J Clin Microbiol ; 51(2): 669-72, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23241373

RESUMO

Long-term typhoid carriers can simultaneously excrete Salmonella enterica serovar Typhi variants with considerable genetic differences, a situation that complicates the interpretation of the subtyping data used in outbreak investigations and disease surveillance.


Assuntos
Portador Sadio , Variação Genética , Salmonella typhi/genética , Febre Tifoide/microbiologia , Idoso de 80 Anos ou mais , Análise por Conglomerados , Surtos de Doenças , Feminino , Genótipo , Humanos , Repetições de Microssatélites , Tipagem Molecular , Salmonella typhi/classificação , Febre Tifoide/epidemiologia
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