Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 29
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Rev Sci Instrum ; 90(8): 084705, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31472637

RESUMO

A low-frequency superconducting cavity is needed in main accelerators for storage ring light sources with ultralow emittance. A compact 166.6 MHz superconducting proof-of-principle cavity was designed adopting a quarter-wave ß = 1 geometry for a High Energy Photon Source (HEPS). It is a 6 GeV diffraction-limited synchrotron light source currently being developed at the Institute of High Energy Physics. The cavity is exceedingly compact in size yet possessing a low resonant frequency. The nearest higher order mode is largely separated from the fundamental, making the cavity an attractive geometry for effective damping of these modes in high current accelerators such as HEPS. The achieved accelerating voltage of 3.0 MV is well beyond the designed 1.5 MV and required 1.2 MV for HEPS operation. High surface electromagnetic fields were reached with excellent rf and mechanical performances, and multipacting barriers were easily processed. This constitutes the first demonstration of a compact low-frequency ß = 1 superconducting cavity for HEPS. The design, fabrication, surface preparation, and cryogenic tests of the cavity are presented.

2.
Metab Brain Dis ; 34(5): 1431-1446, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31286327

RESUMO

The sedentary lifestyle is responsible for the high prevalence of diabetes which also impairs cognition including learning and memory. Various studies have highlighted the learning and memory impairments in rodent models but data regarding the timeline of their development and their correlation to biochemical parameters are scarce. So, the present study was designed to investigate the type of memory which is more susceptible to hyperglycemia and its correlation with biochemical parameters such as inflammatory cytokines, cAMP response element binding (CREB) and protein kinase B (Akt) activation. Hyperglycemia was induced using streptozotocin (STZ, 45 mg/kg i.p.) and confirmed by measuring fasting blood glucose levels after 1 week of STZ injection. Learning and memory deficits were evaluated using the Novel Object Recognition Test (NORT) and Morris water maze (MWM), and correlated with biochemical parameters (TNF-α, IL-1ß, and dopamine) at 3, 6 and 9 weeks. STZ-injected rats after 3 weeks of injection demonstrated moderate hyperglycemia (blood glucose = 7.99 ± 0.62 mM) with intact learning and reference memory; however, their working memory was impaired in MWM. Severe hyperglycemia (blood glucose = 11.51 ± 0.69 mM) accompanied by impaired short, long, and working memory was evident after 6 weeks whereas learning was intact. After 9 weeks of STZ injection, hyperglycemia was more pronounced (13.69 ± 1.43 mM) and accompanied by a learning deficit in addition to short, long, and working memory impairments. The extent of hyperglycemia either in terms of duration or severity resulted in enhanced inflammation, down-regulation of the level of dopamine, protein expression of AKT and CREB, which possibly affected learning and memory negatively.

3.
Sci Total Environ ; 686: 118-130, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31176811

RESUMO

Marine submerged aquatic angiosperms (seagrasses) are declining globally. The species Zostera japonica Asch. & Graebn. is endangered in its native range in Asia, but has been successfully introduced to North America. A large area (1031.8 ha) of Z. japonica meadow has recently been discovered in the intertidal zone of Yellow River Delta, China. This seagrass occurs along both sides of the river mouth, forming dense meadows in turbid water conditions. Seasonal investigations over two years were conducted to examine the distribution, biomass, seed reproduction, seed bank, and population recruitment of the seagrass meadows at three sites in the intertidal zone. The meadows generally showed relatively high coverage, biomass, reproductive effort, and seed production in August. The seed bank was found to be large and contributed to population recruitment. There were significant inter-annual variations overall, and at individual sites. These variations are likely due to winter temperatures, which determine the abundance of overwintering shoots and seedling success. Differences in micro-topography may also play a role in producing variations in seedling success between sites. Microsatellite analysis revealed a high genetic exchange between the two sides of the river mouth. The results indicate that the seagrass bed in the Yellow River Delta shallow waters is in good condition, which can be attributed to its location within a national nature reserve. Establishment of protected areas might act as an effective way to mitigate the anthropogenic disturbance, conserve the seagrass meadows, and then enhance critical ecosystem functions.


Assuntos
Ecossistema , Zosteraceae/crescimento & desenvolvimento , Biomassa , China , Pradaria , Rios
4.
J Thorac Oncol ; 14(3): 445-458, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30476574

RESUMO

INTRODUCTION: Electromagnetic navigation bronchoscopy (ENB) is a minimally invasive technology that guides endoscopic tools to pulmonary lesions. ENB has been evaluated primarily in small, single-center studies; thus, the diagnostic yield in a generalizable setting is unknown. METHODS: NAVIGATE is a prospective, multicenter, cohort study that evaluated ENB using the superDimension navigation system (Medtronic, Minneapolis, Minnesota). In this United States cohort analysis, 1215 consecutive subjects were enrolled at 29 academic and community sites from April 2015 to August 2016. RESULTS: The median lesion size was 20.0 mm. Fluoroscopy was used in 91% of cases (lesions visible in 60%) and radial endobronchial ultrasound in 57%. The median ENB planning time was 5 minutes; the ENB-specific procedure time was 25 minutes. Among 1157 subjects undergoing ENB-guided biopsy, 94% (1092 of 1157) had navigation completed and tissue obtained. Follow-up was completed in 99% of subjects at 1 month and 80% at 12 months. The 12-month diagnostic yield was 73%. Pathology results of the ENB-aided tissue samples showed malignancy in 44% (484 of 1092). Sensitivity, specificity, positive predictive value, and negative predictive value for malignancy were 69%, 100%, 100%, and 56%, respectively. ENB-related Common Terminology Criteria for Adverse Events grade 2 or higher pneumothoraces (requiring admission or chest tube placement) occurred in 2.9%. The ENB-related Common Terminology Criteria for Adverse Events grade 2 or higher bronchopulmonary hemorrhage and grade 4 or higher respiratory failure rates were 1.5% and 0.7%, respectively. CONCLUSIONS: NAVIGATE shows that an ENB-aided diagnosis can be obtained in approximately three-quarters of evaluable patients across a generalizable cohort based on prospective 12-month follow-up in a pragmatic setting with a low procedural complication rate.

5.
Front Plant Sci ; 9: 1545, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30405676

RESUMO

Healthy seagrasses are considered a prime indicator of estuarine and coastal ecosystem function; however, as the only group of flowering plants recolonizing the sea, seagrasses are frequently exposed to anthropogenic heavy metal pollutants, which are associated with high levels of molecular damage. To determine whether biologically relevant concentrations of heavy metals cause systematic alterations in RNA expression patterns, we performed a gene expression study using transcriptome analyses (RNA-seq). We exposed the typical intertidal seagrass Zostera japonica to 0 and 50 µM of copper (Cu), lead (Pb), and cadmium (Cd) under laboratory conditions. A total of 18,266 differentially expressed genes (DEGs) were identified, of which 2001 co-expressed genes directly related by Cu, Pb, and Cd stress. We also examined the effects of short-term heavy metal Cu, Pb, and Cd pulses on the accumulation of metals in Z. japonica and showed metal concentrations were higher in the shoots than in roots. Twelve differentially expressed genes were further analyzed for expression differences using real-time quantitative polymerase chain reaction (RT-qPCR). Our data suggest that as coastal seawater pollution worsens, the sensitive genes identified in this study may be useful biomarkers of sublethal effects and provide fundamental information for Z. japonica resistant gene engineering.

6.
Protein Expr Purif ; 151: 9-17, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29857035

RESUMO

The trans-translation system is recognized as an excellent target for developing new drugs to rapidly sterilize Mycobacterium tuberculosis (TB) infection and significantly shorten TB treatment duration. As a vital component of the trans-translation system for rescuing stalled ribosomes, the SmpB protein from Mycobacterium tuberculosis (MtbSmpB, 1-160 a. a.) mediates tmRNA binding to stalled ribosomes through forming a complex with tmRNA. So far, few works have been conducted to prepare, characterize biophysical properties and determine three-dimensional structure for the full-length MtbSmpB protein. In the present work, we successfully expressed and purified the His-tagged full-length MtbSmpB protein in Escherichia coli with a yield of 26.9 mg from 1 L of Luria Bertani medium. We also obtained MtbSmpB with a yield of 18.5 mg from 1 L of M9 minimal medium. The MtbSmpB protein showed a single band in SDS-PAGE with a molecular weight of ∼20 kDa consistent with the measurement from MALDI-TOF-mass spectrometry. The dynamic light scattering experiment indicated that MtbSmpB existed in a monomeric form. Moreover, both circular dichroism and nuclear magnetic resonance (NMR) experiments exhibited that MtbSmpB was well structured, suggesting that it could be feasible to determine its solution structure by NMR spectroscopy. NMR titration experiments showed that MtbSmpB specifically bound to tmRNA. This work lays the essential basis for further determining the solution structure and dynamics of the full-length MtbSmpB protein.


Assuntos
Proteínas de Bactérias/biossíntese , Mycobacterium tuberculosis/metabolismo , Proteínas de Ligação a RNA/biossíntese , Proteínas de Bactérias/isolamento & purificação , Escherichia coli/genética , Modelos Moleculares , Conformação Proteica , Proteínas de Ligação a RNA/isolamento & purificação
7.
Front Plant Sci ; 9: 221, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29628930

RESUMO

Seeds are important materials for the restoration of globally-threatened marine angiosperm (seagrass) populations. In this study, we investigated the differences between different Ruppia sinensis seed types and developed two feasible long-term R. sinensis seed storage methods. The ability of R. sinensis seeds to tolerate the short-term desiccation and extreme cold had been investigated. The tolerance of R. sinensis seeds to long-term exposure of high salinity, cold temperature, and desiccation had been considered as potential methods for long-term seed storage. Also, three morphological and nine physiological indices were measured and compared between two types of seeds: Shape L and Shape S. We found that: (1) wet storage at a salinity of 30-40 psu and 0°C were the optimal long-term storage conditions, and the proportion of viable seeds reached over 90% after a storage period of 11 months since the seeds were collected from the reproductive shoots; (2) dry condition was not the optimal choice for long-term storage of R. sinensis seeds; however, storing seeds in a dry condition at 5°C and 33 ± 10% relative humidity for 9 months had a relatively high percentage (74.44 ± 2.22%) of viable seeds, consequently desiccation exposure could also be an acceptable seed storage method; (3) R. sinensis seeds would lose vigor in the interaction of extreme cold (-27°C) and desiccation; (4) there were significant differences in seed weight, seed curvature, and endocarp thickness between the two types of seeds. These findings provided fundamental physiological information for R. sinensis seeds and supported the long-term storage of its seeds. Our results may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

8.
Mar Pollut Bull ; 134: 5-13, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29534833

RESUMO

Trace element accumulation is an anthropogenic threat to seagrass ecosystems, which in turn may affect the health of humans who depend on these ecosystems. Trace element accumulation in seagrass meadows may vary temporally due to, e.g., seasonal patterns in sediment discharge from upstream areas. In addition, when several trace elements are present in sufficiently high concentrations, the risk of seagrass loss due to the cumulative impact of these trace elements is increased. To assess the seasonal variation and cumulative risk of trace element contamination to seagrass meadows, trace element (As, Cd, Cr, Cu, Pb, Hg, Mn and Zn) levels in surface sediment and seagrass tissues were measured in the largest Chinese Zostera japonica habitat, located in the Yellow River Estuary, at three sites and three seasons (fall, spring and summer) in 2014-2015. In all three seasons, trace element accumulation in the sediment exceeded background levels for Cd and Hg. Cumulative risk to Z. japonica habitat in the Yellow River Estuary, from all trace elements together, was assessed as "moderate" in all three seasons examined. Bioaccumulation of trace elements by seagrass tissues was highly variable between seasons and between above-ground and below-ground biomass. The variation in trace element concentration of seagrass tissues was much higher than the variation in trace element concentration of the sediment. In addition, for trace elements which tended to accumulate more in above-ground biomass than below-ground biomass (Cd and Mn), the ratio of above-ground to below-ground trace element concentration peaked at times corresponding to high water discharge and high sediment loads in the Yellow River Estuary. Overall, our results suggest that trace element accumulation in the sediment may not vary between seasons, but bioaccumulation in seagrass tissues is highly variable and may respond directly to trace elements in the water column.


Assuntos
Metais/análise , Poluentes Químicos da Água/análise , Zosteraceae/química , Arsênico/análise , Biomassa , China , Ecossistema , Ecotoxicologia , Monitoramento Ambiental/métodos , Estuários , Sedimentos Geológicos/análise , Mercúrio/análise , Rios , Estações do Ano , Zosteraceae/efeitos dos fármacos
9.
Mar Pollut Bull ; 134: 177-185, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28823425

RESUMO

As typical submerged aquatic vegetation, Ruppia species are facing population reductions due to anthropogenic impacts. In this study, we investigated the effects of temperature and salinity on seed germination and seedling establishment of Ruppia sinensis seeds collected from northern China. The effects of seven salinities (0-50) and six water temperatures (0-30°C) on seed germination were investigated to identify the environmental conditions that could potentially limit survival and growth. We found that: 1) optimum seed germination was salinity 5 at 30°C; 2) high salinity (salinity 40-50) and low temperature (0°C) significantly inhibited seed germination; 3) seed germination with increasing temperature showed a bimodal pattern at suitable salinities (5-10); 4) storing seeds at high salinities (40-50) or low temperature (0°C) promoted germination after transferal to optimal germination conditions. These findings may serve as useful information for R. sinensis habitat establishment and restoration programs.


Assuntos
Alismatales/crescimento & desenvolvimento , Germinação , Plântula/crescimento & desenvolvimento , Alismatales/fisiologia , China , Estuários , Salinidade , Sementes/crescimento & desenvolvimento , Temperatura
10.
Sheng Li Xue Bao ; 69(2): 159-166, 2017 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-28435974

RESUMO

To investigate whether the extract of Dendrobium nobile Lindl (DNL) has an antidepressant effect on chronic unpredictable mild stress (CUMS)-induced depressive mice, 72 BALB/c male mice were randomly divided into the control group, the CUMS model group, the extract of DNL groups (50, 100 and 200 mg/kg DNL, i.g.) and the paroxetine group (10 mg/kg, i.g.). The different doses of DNL or the paroxetine was administered orally once daily to CUMS mice for 8 weeks (containing two-week preventive medication before the modeling). The same volume of distilled water was given to the control group and the CUMS group. Except for the control group, the other mice were exposed to chronic stress for 35 days. Behavioral tests were performed by using the sucrose preference test (SPT), the novelty-suppressed feeding (NSF) test, the tail suspension test (TST), and the forced swim test (FST). The levels of dopamine (DA) and 5-hydroxytryptamine (5-HT) were measured by the liquid chromatography-mass spectrometer (LC-MS)/MS. Compared with the control group, obvious behavioral changes were observed in the CUMS group after 5-week CUMS, including a decrease in the sucrose consumption, an increase in the latency to feeding in the NSF test and a prolongation of the immobility time in the TST. Compared with the CUMS group, the application of DNL resulted in a dose-dependent increase in sucrose consumption (P < 0.01) as paroxetine (10 mg/kg) did and a significant dose-dependent decrease in the latency to feeding in the NSF test (P < 0.05). In the TST, the application of paroxetine (10 mg/kg) and the high-dose DNL (200 mg/kg) obviously decreased the immobility time when compared with the CUMS group (P < 0.05). In the FST, compared with the CUMS group, all the groups had no significant differences in the immobility time (P > 0.05). In addition, in the hippocampus and cortex, the levels of 5-HT and DA were significantly decreased in the CUMS group compared with the control group (P < 0.05). In comparison with the CUMS group, paroxetine obviously increased the DA levels in the hippocampus and the cortex and the 5-HT level in the hippocampus (P < 0.05). DNL (50 and 200 mg/kg) significantly increased the DA level in cerebral cortex of the brain, and DNL (100 and 200 mg/kg) increased the DA level in the hippocampus. The 5-HT level in the 200 mg/kg DNL group was notably increased in both two brain regions (P < 0.05), but the 5-HT level in the 100 mg/kg DNL group was significantly increased only in the hippocampus (P < 0.01). These findings indicate that the extract of DNL has an antidepressant-like effect on CUMS-induced depressive mice and its mechanism may be related to the changes in DA and 5-HT in the hippocampus and cortex.


Assuntos
Antidepressivos/farmacologia , Dendrobium/química , Depressão/tratamento farmacológico , Extratos Vegetais/farmacologia , Estresse Psicológico/tratamento farmacológico , Animais , Modelos Animais de Doenças , Dopamina/sangue , Elevação dos Membros Posteriores , Hipocampo/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Serotonina/sangue , Sacarose , Natação
11.
BMC Med Genet ; 18(1): 2, 2017 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-28061824

RESUMO

BACKGROUND: Nance-Horan Syndrome (NHS) (OMIM: 302350) is a rare X-linked developmental disorder characterized by bilateral congenital cataracts, with occasional dental anomalies, characteristic dysmorphic features, brachymetacarpia and mental retardation. Carrier females exhibit similar manifestations that are less severe than in affected males. METHODS: Here, we report a four-generation Chinese family with multiple affected individuals presenting Nance-Horan Syndrome. Whole-exome sequencing combined with RT-PCR and Sanger sequencing was used to search for a genetic cause underlying the disease phenotype. RESULTS: Whole-exome sequencing identified in all affected individuals of the family a novel donor splicing site mutation (NM_198270: c.1045 + 2T > A) in intron 4 of the gene NHS, which maps to chromosome Xp22.13. The identified mutation results in an RNA processing defect causing a 416-nucleotide addition to exon 4 of the mRNA transcript, likely producing a truncated NHS protein. CONCLUSIONS: The donor splicing site mutation NM_198270: c.1045 + 2T > A of the NHS gene is the causative mutation in this Nance-Horan Syndrome family. This research broadens the spectrum of NHS gene mutations, contributing to our understanding of the molecular genetics of NHS.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Catarata/congênito , Doenças Genéticas Ligadas ao Cromossomo X/genética , Mutação , Proteínas Nucleares/genética , Anormalidades Dentárias/genética , Catarata/genética , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Linhagem , Sítios de Splice de RNA , Análise de Sequência de DNA/métodos
12.
Mar Pollut Bull ; 109(1): 325-333, 2016 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-27287861

RESUMO

To investigate the potential influences of anthropogenic pollutants, we evaluated the responses of the intertidal seagrass Zostera japonica to three heavy metals: copper (Cu), lead (Pb), and cadmium (Cd). Z. japonica was exposed to various concentrations of Cu, Pb, and Cd (0, 0.5, 5, 50µM) over seven days. The effects were then analyzed using the antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione peroxidase (GPX), and lipid peroxidation measured using malondialdehyde (MDA) as proxy. Metal accumulation in the above-ground tissues and phenotypic changes were also investigated. Our results revealed that heavy metal concentration increased in seagrass exposed to high levels of metals. Z. japonica has great potential for metal accumulation and a suitable candidate for the decontamination of moderately Cu contaminated bodies of water and can also potentially enhanced efforts of environmental decontamination, either through phytoextraction abilities or by functioning as an indicator for monitoring programs that use SOD, CAT, GPX, POD and MDA as biomarkers.


Assuntos
Monitoramento Ambiental/métodos , Metais Pesados/toxicidade , Zosteraceae/fisiologia , Antioxidantes , Biomarcadores/metabolismo , Cádmio/análise , Cádmio/toxicidade , Catalase/metabolismo , Cobre/análise , Cobre/toxicidade , Glutationa Peroxidase/metabolismo , Chumbo/análise , Chumbo/toxicidade , Peroxidação de Lipídeos/efeitos dos fármacos , Malondialdeído/metabolismo , Metais Pesados/análise , Estresse Oxidativo , Superóxido Dismutase/metabolismo
13.
Environ Pollut ; 214: 822-830, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27155100

RESUMO

Seawater flue gas desulfurization (SFGD) systems are commonly used to remove acidic SO2 from the flue gas with alkaline seawater in many coastal coal-fired power plants in China. However, large amount of mercury (Hg) originated from coal is also transferred into seawater during the desulfurization (De-SO2) process. This research investigated Hg isotopes in seawater discharged from a coastal plant equipped with a SFGD system for the first time. Suspended particles of inorganic minerals, carbon residuals and sulfides are enriched in heavy Hg isotopes during the De-SO2 process. δ(202)Hg of particulate mercury (PHg) gradually decreased from -0.30‰ to -1.53‰ in study sea area as the distance from the point of discharge increased. The results revealed that physical mixing of contaminated De-SO2 seawater and uncontaminated fresh seawater caused a change in isotopic composition of PHg isotopes in the discharging area; and suggested that both De-SO2 seawater and local background contributed to PHg. The impacted sea area predicted with isotopic tracing technique was much larger than that resulted from a simple comparison of pollutant concentration. It was the first attempt to apply mercury isotopic composition signatures with two-component mixing model to trace the mercury pollution and its influence in seawater. The results could be beneficial to the coal-fired plants with SFGD systems to assess and control Hg pollution in sea area.


Assuntos
Recuperação e Remediação Ambiental , Isótopos de Mercúrio/análise , Material Particulado/análise , Água do Mar/análise , Poluição Química da Água/prevenção & controle , China , Carvão Mineral , Monitoramento Ambiental , Gases/química , Resíduos Industriais , Centrais Elétricas , Dióxido de Enxofre/química , Águas Residuárias/análise
14.
Sci Total Environ ; 541: 435-443, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26410718

RESUMO

Globally, seagrass habitats are decreasing due to both natural and environmental contaminations by human activities, including heavy metal pollution. To expand the global seagrass detection network, this study reports the spatial distributions of Zostera japonica seagrass habitats in the Yellow River Estuary, China. In addition, heavy metal concentrations of Z. japonica tissue, sediment, and surface seawater were analyzed to determine the bioaccumulation and consequent ecological risk to Z. japonica habitats due to the effects of heavy metals. It was found that concentrations of heavy metals were 1.00-2.03 times higher in seagrass-rooted sediment than in adjacent non-seagrass sediment, except for Mn (with a factor of 0.99). Pb and Hg concentrations in sediments exceeded background values more than the other heavy metals, by factors of 1.74 and 1.24, respectively. Metal concentrations in the surrounding seawater were 2.60-4.63 times higher at seagrass sites than at non-seagrass sites, except for Hg (factor of 0.97). Metal concentrations were much higher in seagrass tissues than in the sediment (e.g., bioconcentration factor of Cd is 30.95). Pb concentrations in water may cause the greatest adverse reactions among aquatic organisms, while As, Cr, Hg, Mn and Cu in sediments may occasionally cause negative ecological effects. Z. japonica showed higher bioaccumulation of Cd and Pb in the above-ground tissues. Among other recent studies of seagrasses from other parts of the world, Cd concentrations are similar to the results of the present study, but Pb concentration in present study is higher than in other studies. In conclusion, Pb and As in the surrounding environment present potential risks to the seagrass habitats of the Yellow River Estuary, China.


Assuntos
Ecossistema , Monitoramento Ambiental , Estuários , Metais Pesados/análise , Poluentes Químicos da Água/análise , Zosteraceae/química , China , Medição de Risco
15.
Artigo em Chinês | MEDLINE | ID: mdl-26696471

RESUMO

OBJECTIVE: To explore the effect of chronological age and acoustic device in cognitive development of congenital hearing-impaired infants and toddlers, and analyze the correlation of abilities in cognitive development with other factors. METHODS: Depending on chronological age (1 year old group and 2 years old group) and acoustic device (hearing aids and cochlear implantation), locomotor, personal-social, hearing and speech, hand and eye co-ordination, performance tests in Griffith Cognitive Development Scale were used to assess the cognitive development of 80 hearing-impaired infants and toddlers aged 0-2 years, including before intervention (0 month), after intervention (6, 12 months). Datas were analyzed by Repeated Measurements and Pearson Correlation Test. RESULTS: During 1 year hearing intervention and rehabilitation, hearing and speech, performance and cognitive were extremely significant difference for each phase of early intervention (P < 0.01), the development of locomotor, personal-social, hand and eye co-ordination were no significant difference (P > 0.05). Personal-Social in 1 year old group with hearing impairment was much higher than 2 years old group P < 0.05). Hearing and speech in cochlear implanted group with hearing loss was much higher than hearing aids group. Cognitive development was positive correlation with various region development P < 0.01), and was negatively correlated with chronological age (P > 0.05). CONCLUSIONS: Cognitive development is proportional to recovery time. The chronological age of early intervention obviously affect deaf children's cognitive development. The ability of hearing and speech in cochlear implanted children is superior to children with hearing aids in severe and profound hearing impaired children.


Assuntos
Desenvolvimento Infantil , Cognição , Surdez/congênito , Pré-Escolar , Implante Coclear , Implantes Cocleares , Surdez/reabilitação , Audição , Auxiliares de Audição , Perda Auditiva/congênito , Perda Auditiva/reabilitação , Testes Auditivos , Humanos , Lactente , Estudos Longitudinais , Destreza Motora , Fala , Percepção da Fala
16.
J Nanosci Nanotechnol ; 15(6): 4596-603, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26369086

RESUMO

In this work, magnetic particles were firstly protected by oleic acid, and then polymers, the polymers was prepared with allyl-thiourea as the functional monomer, ethyleneglycol dimethacrylate as the cross-linking agent, 2,2-azobisisobutyronitrile as the initiator, and acetonitrile as the solvent. The magnetic polymers were analyzed by FT-IR, X-ray diffraction, and a vibrating sample magnetometer to obtain the morphological and magnetic properties. The adsorption of phosphate on the magnetic polymers was investigated, including pH effect, initial concentration, and temperature. The results proved that the adsorbent was paramagnetic and successfully loaded with the poly-thiourea group. The data was well fitted to the Langmuir adsorption isotherm, and the maximum adsorption capacity was 55.20 mg-P g(-1). Furthermore, desorption of phosphate from the adsorbent could be achieved efficiently by 0.5 mol L(-1) NaOH, reusability was studied by repeating adsorption-desorption cycles five times.

17.
Microb Pathog ; 83-84: 35-40, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25959527

RESUMO

The efforts were focused on exploring alternative pneumococcal vaccine strategies, aimed at addressing the shortcomings of existing formulations, without compromising efficacy. Our strategy involved the use of the carrier protein, pneumococcal surface protein A (PspA), conjugated with capsular polysaccharides (CPS), to provide effective and non-serotype-dependent protection. In this study, we generated a stable Escherichia coli construct expressing functional PspA from a capsular serotype 6B strain and confirmed it belonging to family 1, which was conjugated with CPS. The distribution of anti-CPS antibody response was almost completely of IgG2a subclass followed by IgG3 and low level of IgG1 subclass, but that of anti-PspA IgG subclass antibodies was almost equal IgG1 and IgG2a subclasses. Though PspA was less conspicuous on the surface of pneumococci than the capsule, the antibodies induced with CPS-rPspA conjugate possessed more accessibility to the surface of Streptococcus pneumoniae serotype 6B and 19F (the same family 1 PspA). By survival experiment, the result suggested that the level of cross-protection after immunized with the conjugate was more measurable within the same family 1. The CPS-rPspA conjugate not only induced CPS-specific protection but also provided PspA specific cross-protection.


Assuntos
Proteínas de Bactérias/imunologia , Infecções Pneumocócicas/prevenção & controle , Vacinas Pneumocócicas/imunologia , Polissacarídeos Bacterianos/imunologia , Streptococcus pneumoniae/imunologia , Animais , Anticorpos Antibacterianos/sangue , Proteínas de Bactérias/genética , Proteção Cruzada , Modelos Animais de Doenças , Escherichia coli/genética , Escherichia coli/metabolismo , Imunoglobulina G/sangue , Camundongos Endogâmicos BALB C , Infecções Pneumocócicas/imunologia , Infecções Pneumocócicas/microbiologia , Vacinas Pneumocócicas/administração & dosagem , Vacinas Pneumocócicas/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Análise de Sobrevida , Vacinas Conjugadas/administração & dosagem , Vacinas Conjugadas/imunologia , Vacinas Conjugadas/isolamento & purificação , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/isolamento & purificação
18.
Hemoglobin ; 39(1): 18-23, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25548039

RESUMO

We have developed a new method for non-invasive prenatal testing (NIPT) of paternally inherited fetal mutants for ß-thalassemia (ß-thal). Specially designed primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR) were used to detect four major mutations [IVS-II-654, HBB: c.316-197C > T; codon 17 (A > T), HBB: c.52A > T; -28 (A > G), HBB: c.-78A > G and codons 41/42 (-TTCT), HBB: c.126_129delCTTT] causing ß-thal in China. The PIRA-PCR assay was first tested in a series of mixed DNA with different concentrations and mixed proportions. Subsequently, this assay was further tested in 10 plasma DNA samples collected from pregnant women. In the DNA mixture simulation test, the PIRA-PCR assay was able to detect 3.0% target genomic DNA (gDNA) mixed in 97.0% wild-type gDNA isolated from whole blood. For plasma DNA testing, the results detected by PIRA-PCR assay achieved 100.0% consistency with those obtained from the amniocentesis analysis. This new method could potentially be used for NIPT of paternally inherited fetal mutants for ß-thal.


Assuntos
Mutação , Reação em Cadeia da Polimerase/métodos , Diagnóstico Pré-Natal/métodos , Globinas beta/genética , Talassemia beta/genética , Sequência de Bases , Análise Mutacional de DNA/métodos , Primers do DNA/genética , Feminino , Humanos , Masculino , Gravidez , Talassemia beta/diagnóstico
19.
Eur Spine J ; 23(12): 2711-7, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25169142

RESUMO

PURPOSE: The purpose of this study is to report a new technique and assess clinical outcome of compressive fractures with posterior vertebral defect treated by percutaneous vertebroplasty combined with the spinal external fixator. METHOD: 80 patients (32 males and 48 females), ranging from 62 to 88 years old with the mean age of 71.5 years, underwent surgery for the compressive fractures with posterior vertebral defect by percutaneous vertebroplasty combined with the spinal external fixator. All patients were diagnosed to have fresh compressive fractures with osteoporosis and posterior vertebral defect shown on roentgenograms, computed tomography scans or magnetic resonance imaging preoperatively. They underwent spinal external fixation firstly to be fixed and restored, then to be carried out percutaneous vertebroplasty. The mean follow-up was 24 months (16-42 months). Spinal canal encroachment, spinal cobb angle and vertebral body height loss were measured to assess clinical outcome before and after surgery, at the final follow-up. The Visual Analogue Scale and Oswestry Disability Index were used for pain and functional assessment. In all cases, preoperative and postoperative radiographs and magnetic resonance imaging were obtained. RESULTS: The average time of surgery was 88 min (75-115 min). The mean blood loss was 10 ml (6-12 ml) during surgery. The anterior height loss of vertebral body decreased significantly from 79.3 ± 11% before surgery to 8.0 ± 5.2% after surgery, and 7.6 ± 6.0% at the final follow-up. The spinal canal encroachment significantly reduced from 19.9 ± 2.6 % preoperatively to 4.0 ± 0.7% postoperatively, 4.1 ± 0.7% at the final follow-up. The Cobb angle was corrected from 25.8 ± 7.9° primarily to 8.2 ± 4.1° postoperatively, 7.8 ± 3.1° at the final follow-up. There were significant differences (p < 0.05) among them before and after the surgery. Postoperative VAS and Oswestry scores were both significantly different from the preoperative and follow-up (p < 0.05). CONCLUSION: The preliminary results are encouraging, showing that the spinal external fixator combined with percutaneous vertebroplasty was a safe and effective method to treat the osteoporotic compressive fractures with posterior vertebral defect.


Assuntos
Fixadores Externos , Fraturas por Compressão/cirurgia , Vértebras Lombares/lesões , Fraturas por Osteoporose/cirurgia , Fraturas da Coluna Vertebral/cirurgia , Vértebras Torácicas/lesões , Vertebroplastia/métodos , Idoso , Idoso de 80 Anos ou mais , Feminino , Fraturas por Compressão/diagnóstico , Humanos , Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/patologia , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Fraturas por Osteoporose/diagnóstico , Medição da Dor , Radiografia , Fraturas da Coluna Vertebral/diagnóstico , Vértebras Torácicas/diagnóstico por imagem , Vértebras Torácicas/patologia , Resultado do Tratamento
20.
PLoS One ; 8(5): e62328, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23658724

RESUMO

OBJECTIVE: To compare the measurements of glomerular filtration rate (GFR) determined by (99m)Tc-diethylene triamine pentaacetic acid ((99m)Tc-DTPA) renal dynamic imaging with those estimated by Chronic Kidney Disease Epidemiology Collaboration (CDK-EPI) equation and to identify a more accurate measurement of GFR of chronic kidney disease (CKD) patients in clinical practice. METHODS: The GFR was determined simultaneously by 3 methods: (a) dual plasma sample clearance method (tGFR); (b) renal dynamic imaging method (dGFR); (c) CDK-EPI equation (eGFR). The tGFR was employed as the reference method. The correlation, regression, and limit of agreement of dGFR and eGFR were used to demonstrate the validity of the two methods. The comparison of bias, precision, and accuracy between dGFR and eGFR was analyzed to identify the most suitable method. The analysis of bias, precision and accuracy was repeated after stratifying patients by a measured tGFR cutpoint of 60 ml·min(-1)·(1.73 m(2))(-1). RESULTS: A total of 149 patients were enrolled. Both dGFR and eGFR correlated well with tGFR and the regression equation of dGFR and eGFR against tGFR was respectively Y = -4.289+0.962X (r = 0.919; RMSE = 14.323 ml.min(-1). (1.73 m(2))(-1); P<0.001) and Y = 2.462+0.914X (r = 0.909; RMSE = 15.123 ml.min(-1). (1.73 m(2))(-1); P<0.001). In addition, Bland-Altman analysis showed preferable agreement between the two methods and the reference method. The comparison revealed that eGFR, compared with dGFR, showed better performance on bias and 50% accuracy and similar performance on other indexes in the whole cohort and the lower-GFR subgroup, whereas in the higher-GFR subgroup the difference of the two methods was not significant in all parameters. CONCLUSIONS: Although both CDK-EPI equation and renal dynamic imaging can be used to determine the GFR of CKD patients, CDK-EPI equation is more accurate than renal dynamic imaging. As a result, (99m)Tc-DTPA renal dynamic imaging may be unsuitable to be used as the reference method in investigating the validity of CDK-EPI equation.


Assuntos
Taxa de Filtração Glomerular , Compostos Radiofarmacêuticos , Insuficiência Renal Crônica/fisiopatologia , Pentetato de Tecnécio Tc 99m , Algoritmos , Feminino , Humanos , Rim/diagnóstico por imagem , Rim/fisiopatologia , Testes de Função Renal/métodos , Masculino , Pessoa de Meia-Idade , Cintilografia , Insuficiência Renal Crônica/diagnóstico por imagem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA