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1.
Br J Surg ; 2020 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-32246475

RESUMO

BACKGROUND: Hepatic vein tumour thrombus (HVTT) is a major determinant of survival outcomes for patients with hepatocellular carcinoma (HCC). An Eastern Hepatobiliary Surgery Hospital (EHBH)-HVTT model was established to predict the prognosis of patients with HCC and HVTT after liver resection, in order to identify optimal candidates for liver resection. METHODS: Patients with HCC and HVTT from 15 hospitals in China were included. The EHBH-HVTT model with contour plot was developed using a non-linear model in the training cohort, and subsequently validated in internal and external cohorts. RESULTS: Of 850 patients who met the inclusion criteria, there were 292 patients who had liver resection and 198 who did not in the training cohort, and 124 and 236 in the internal and external validation cohorts respectively. Contour plots for the EHBH-HVTT model were established to predict overall survival (OS) rates of patients visually, based on tumour diameter, number of tumours and portal vein tumour thrombus. This differentiated patients into low- and high-risk groups with distinct long-term prognoses in the liver resection cohort (median OS 34·7 versus 12·0 months; P < 0·001), internal validation cohort (32·8 versus 10·4 months; P = 0·002) and external validation cohort (15·2 versus 6·5 months; P = 0·006). On subgroup analysis, the model showed the same efficacy in differentiating patients with HVTT in peripheral and major hepatic veins, the inferior vena cava, or in patients with coexisting portal vein tumour thrombus. CONCLUSION: The EHBH-HVTT model was accurate in predicting prognosis in patients with HCC and HVTT after liver resection. It identified optimal candidates for liver resection among patients with HCC and HVTT, including tumour thrombus in the inferior vena cava, or coexisting portal vein tumour thrombus.

2.
Zhonghua Yi Xue Za Zhi ; 100(15): 1121-1122, 2020 Apr 21.
Artigo em Chinês | MEDLINE | ID: mdl-32311874
3.
J Colloid Interface Sci ; 560: 34-39, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31648084

RESUMO

Transition metal oxides show great potential as electrocatalysts, owing to the low cost and rich chemical states. However, the limited surface areas, low intrinsic activity and poor hydrogen evolution reaction (HER) activity greatly restrict the application for overall water splitting. Herein, we have constructed S doped NiCo2O4 nanosheet arrays by Ar plasma (Ar-NiCo2O4|S) to enhance active sites and boost catalytic kinetics. Consequently, the Ar-NiCo2O4|S shows the improved performances for HER and oxygen evolution reaction (OER). Further, as bifunctional electrocatalysts, Ar-NiCo2O4|S exhibit a voltage of 1.63 V at 10 mA cm-2, as well as good stability.

4.
Zhonghua Fu Chan Ke Za Zhi ; 54(12): 826-832, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31874472

RESUMO

Objective: To analyze risk factors, cardiovascular complications, time of death, gestational age of delivery and offspring outcomes in the maternal deaths with cardiovascular diseases (CVD). Methods: Totally 4 112 cases of pregnant women with CVD in Shanghai obstetric heart disease intensive care unit within 26 years (from January 1993 to December 2018) were collected, and 20 maternal deaths within these cases were analyzed retrospectively. Results: (1) Among the 20 deaths, structural heart diseases accounted for 90% (18/20), pregnancy induced heart diseases was 10% (2/20) while there was no dysfunctional heart disease. The mortality of pregnant women with CVD was 0.486% (20/4 112). (2) The following risk factors were common in these women, getting pregnant without counselling (95%, 19/20) , New York Heart Association classⅢ or Ⅳcardiac function (70%, 14/20), complicated with pulmonary hypertension (75%, 15/20) and prior heart events (60%, 12/20). And 85% (17/20) deaths occurred in puerperium, 15% (3/20) occurred before labor,while no death occurred during labor. And 65% (13/20) deaths died due to heart failure, 20% (4/20) deaths were due to pulmonary hypertension crisis, 5% (1/20) died on sudden cardiac arrest, rupture of aortic dissection and sudden death, respectively. Conclusions: Women with CVD should get pregnant after strict evaluation. Pulmonary hypertension is one of the most severe contraindications to pregnancy, especially in patients with moderate to severe pulmonary hypertension. The puerperium period is a critical period that threatens the safety of these patients. Since heart failure is the most common cause of death, it is necessary to prevent and treat heart failure and to monitor heart function dynamically, especially in those with structural abnormal heart diseases. Moreover, it is also of importance to standardize antenatal care and to identify the severity of heart diseases in time.


Assuntos
Doenças Cardiovasculares/mortalidade , Unidades de Terapia Intensiva/estatística & dados numéricos , Morte Materna , Mortalidade Materna , Complicações Cardiovasculares na Gravidez/etiologia , Adulto , Causas de Morte , China/epidemiologia , Feminino , Humanos , Serviços de Saúde Materna/organização & administração , Mortalidade Materna/tendências , Gravidez , Complicações Cardiovasculares na Gravidez/diagnóstico , Complicações Cardiovasculares na Gravidez/mortalidade , Resultado da Gravidez , Estudos Retrospectivos , Fatores de Risco
5.
Zhonghua Wai Ke Za Zhi ; 57(8): 596-600, 2019 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-31422629

RESUMO

Objective: To investigate the microbiological test, antibiotic sensitivity and surgical treatment of periprosthetic joint infection(PJI) cases in post total hip arthroplasty (THA) and total knee arthroplasty (TKA) patients. Methods: A retrospective cross-sectional survey was conducted on 318 patients who underwent THA or TKA in 9 clinical centers in Beijing from January 2014 to December 2016.The data of microbiology, antibiotic sensitivity and surgical treatment were collected.The average age of patients was (62.3±13.1) years old (range: 21-86 years old), including 145 males and 173 females.The body mass index was (25.6±3.8) kg/m (2) (range: 15.6-38.1 kg/m(2)). Results: In total, 318 patients had microorganisms detected by periprosthetic tissue culture or synovial fluid culture, 209 cases (65.7%) had Gram-positive bacteria, 29 cases (9.1%) had Gram-negative bacteria, 10 cases (3.1%) had fungi, 3 cases (0.9%) had non-tuberculous mycobacteria, 72 cases (22.6%) were negative, 69 cases (21.7%) had methicillin-resistant bacteria. The antibiotic sensitivity results showed that the overall resistance rate of penicillin, cefuroxime, amoxicillin+clavulanic acid was 79.9%, 69.9%, and 68.1%, respectively; meropenem, vancomycin, and linezolid resistance rate was 0. For the treatment methods of hip and knee PJI, two-stage revision surgery acounted for 72.9% (108/148) and 64.1% (109/170), respectively. One-stage revision surgery accounted for 21.6% (32/148) and 7.6% (13/170), and open debridement surgery accounted for 4.7%(7/148) and 26.4% (45/170). Conclusions: Gram-positive bacteria was still the main pathogen of PJI.The methicillin-resistant bacteria and rare bacteria should be payed attention to. The Majority of hip and knee PJI cases were treated by two-stage revision surgery.


Assuntos
Artroplastia de Quadril/efeitos adversos , Artroplastia do Joelho/efeitos adversos , Infecções Relacionadas à Prótese/microbiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Pequim , Estudos Transversais , Resistência Microbiana a Medicamentos , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Infecções Relacionadas à Prótese/tratamento farmacológico , Infecções Relacionadas à Prótese/etiologia , Infecções Relacionadas à Prótese/cirurgia , Reoperação , Estudos Retrospectivos , Adulto Jovem
6.
Eur Rev Med Pharmacol Sci ; 23(13): 5795-5801, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31298331

RESUMO

OBJECTIVE: Recently, long non-coding ribonucleic acids (lncRNAs) have attracted more attention for their roles in tumor progression. The aim of this study was to investigate the exact role of lncRNA MIAT in the progression of non-small cell lung cancer (NSCLC) and to explore the possible underlying mechanism. PATIENTS AND METHODS: MIAT expression in NSCLC tissue samples was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The association between the expression of MIAT and the prognosis of NSCLC patients were explored. Furthermore, the wound healing assay and the transwell assay were conducted in vitro. In addition, the luciferase assay and the RNA immunoprecipitation assay (RIP) were used to elucidate the underlying mechanism. RESULTS: The MIAT expression in NSCLC tissues was significantly higher than that of the corresponding normal tissues. Meanwhile, the MIAT expression was associated with the overall survival time of NSCLC patients. The migration and invasion of cells were significantly promoted after MIAT was over-expressed in vitro. Meanwhile, the cell migration and cell invasion were obviously remarkedly inhibited after MIAT knock-down in vitro. Bioinformatics analysis predicted that microRNA-1246 (miR-1246) was as a novel target for MIAT. The expression of miR-1246 was significantly down-regulated or up-regulated after the overexpression or down-expression of MIAT, respectively. Further mechanism assays showed that miR-1246 was a direct target of MIAT in NSCLC. CONCLUSIONS: MIAT enhanced the NSCLC cell migration and invasion via targeting miR-1246, which might be a potential biomarker in NSCLC.

7.
Zhonghua Wai Ke Za Zhi ; 57(1): 39-43, 2019 Jan 01.
Artigo em Chinês | MEDLINE | ID: mdl-30612393

RESUMO

At present, several clinical practice guidelines for osteoarthritis have been developed. Although contradictions about some recommendations are still in dispute, large number of clinical practice guidelines recommended core treatments, namely education, weight loss and exercise therapy. Thus, the diagnosis and treatment of primary osteoarthritis should focus on the above three treatments. However, we have to develop the clinical practice guidelines for osteoarthritis in primary hospital, based on the characteristics of osteoarthritis in China, the burden of disease, the health literacy of patients and the clinical decision-making of diagnosis and treatment of primary osteoarthritis in primary medical care.We suggest to formulate guidelines for the diagnosis and treatment of osteoarthritis in primary medical care to regulate primary interventions.


Assuntos
Osteoartrite , Atenção Primária à Saúde , China , Terapia por Exercício , Humanos , Osteoartrite/diagnóstico , Osteoartrite/terapia , Osteoartrite do Joelho , Perda de Peso
9.
Zhonghua Yi Xue Za Zhi ; 98(27): 2152-2156, 2018 Jul 17.
Artigo em Chinês | MEDLINE | ID: mdl-30032516

RESUMO

Objective: To evaluate the effect of patient education and tailored exercise in the knee osteoarthritis (OA) patients. Methods: One hundred and eighty-five patients with knee OA were enrolled in this ongoing prospective cohort study at Arthritis Clinic and Research Center, Peking University People's Hospital since May, 2016.The treatment consisted of two one-hour sessions of patient education and six weeks of supervised, in-group, tailored exercise.The contents of the patient education were evidence-based and its overall focus was to increase the knowledge of the participants regarding OA and how to treat it.The exercise program was a neuromuscular exercise based on biomechanical and neuromuscular principles that aimed at restoring a neutral functional alignment of the lower extremities by improving sensorimotor control and obtaining compensatory functional stability.The outcomes were evaluated at baseline, 3 and 6 months, which included pain on the visual analogue scale (VAS, 0-100), quality of life on the Euro-quality of life-5 Dimensional (EQ-5D) VAS form, Knee Injury and Osteoarthritis Outcome Score (KOOS), 30-second chair stand test, 40-meter walk test and use of analgesics.The participants were also asked to rate their opinion of this program on a five-point scale (very satisfactory, satisfactory, neither satisfactory nor dissatisfactory, dissatisfactory, very dissatisfactory). One way analysis of variance was applied to compare the outcomes at baseline and 3 and 6 months follow-up. Results: One hundred and forty-two participants completed the 6-month follow-up.There were significant improvements in pain VAS, EQ-5D-VAS, 30-second chair stand test and 40-meter walk test at the follow-up at 3 and 6 months when compared with those at baseline (F=22.9, 8.4, 3.4, 4.1, 3.8, all P<0.05). The rate of analgesics use in the past two week at baseline was 43.2%, and it decreased to 18.9% at 6-month follow-up (P<0.001). Most participants (71.1%, 101 out of 142) rated the program as 'Very Satisfactory' and the rest (28.9%, 41 out of 142) rated the program as 'Satisfactory'. Conclusion: This program comprising the group patient education and tailored exercise therapy is effective in relieving pain, improving the function and quality of life in patients with knee OA.


Assuntos
Terapia por Exercício , Osteoartrite do Joelho , Exercício Físico , Humanos , Joelho , Medição da Dor , Estudos Prospectivos , Qualidade de Vida
10.
Zhonghua Fu Chan Ke Za Zhi ; 53(4): 221-226, 2018 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-29747265

RESUMO

Objective: To analyze the effect of maternal age on pregnancy outcome in patients with structural heart disease. Methods: Totally 986 patients diagnosed with structural heart disease who had their delivery at Renji Hospital from January 1st, 2003 to December 31st, 2016 were divided into 5 groups according to maternal age. Sixteen (1.6%) were in age group <20 years, 204 (20.7%) were in age group 20-24 years, 461 (46.8%) were in age group 25-29 years, 222 (22.5%) was in age group 30-34 years and 83 (8.4%) was in age group ≥35 years. The incidence of cardiac, obstetric and fetal complications among these groups were analyzed. Results: Among the 986 patients with structural heart disease, 82 (8.3%) experienced adverse cardiac events (some patients had more than 1 complication) , including infective endocarditis (0.4%, 4/986) , malignant arrhythmia (3.3%, 33/986) , heart failure (5.3%, 52/986) , cardiac death (0.9%, 9/986) . The incidence of cardiac complications were respectively 12.5%,11.3%,4.8%,10.4% and 14.5% in each age group, with statistically significant differences when compared with one another (P=0.004) . There was no statistically significant difference in the incidence of obstetric complications (P>0.05) . The incidence of fetal complications in each group showed statistically significant differences (P=0.001) ; which was 25.0%,30.4%,24.1%,29.3% and 47.0% respectively. Conclusions: Age group 25- 29 years presented the lowest risk of cardiac complications and is potentially the optimal age for pregnancy and delivery. Age group <20 or ≥35 years is at higher risk for cardiac complications. The highest rate of fetal complications is in age group ≥35 years.


Assuntos
Idade Materna , Complicações Cardiovasculares na Gravidez/epidemiologia , Resultado da Gravidez/epidemiologia , China/epidemiologia , Endocardite/epidemiologia , Endocardite Bacteriana/epidemiologia , Feminino , Cardiopatias/epidemiologia , Humanos , Incidência , Gravidez
11.
Zhonghua Fu Chan Ke Za Zhi ; 53(2): 77-81, 2018 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-29534374

RESUMO

Objective: To observe and analyze the difference of serum immunoglobulin IgA, IgG, IgM, ß2-microglobulin and transferrin in pre-eclampsia (PE) and pregnancies complicated with chronic kidney disease. Methods: Totally 46(40.0%) pregnancies with PE (PE group), 36(31.3%) pregnancies with chronic kidney disease (chronic kidney disease group) and 33(28.7%) normal pregnancies with normal blood pressure and proteinuria without any complication (control group) delivered in Renji Hospital were recruicted in this study from February 2017 to July 2017. Serum IgA, IgG, IgM, ß2-microglobulin and transferrin levels were detected. Correlation tests were conducted between these indicators and blood pressure, 24 hours proteinuria value and delivery weeks. Results: (1) Comparison of general situation of pregnancies in the 3 groups: there were no significant difference in the age and child bearing history between the 3 groups (all P>0.05), while there was a significant difference in the blood pressure and deliver week (all P<0.01). There was no significant difference in 24 hours proteinuria values between PE group and chronic kidney disease group (Z=-0.187, P=0.852). (2) Comparison of serum immunoglobulin, ß2-microglobulin and transferrin levels in pregnant women with three groups: serum IgA level in chronic kidney disease group was significantly higher than those in PE and control groups [(2.4±0.9) vs (1.8±0.9) vs (1.6±0.6) g/L; F=9.959, P<0.01]. The serum IgG and IgM values had no significant difference between the 3 groups (all P>0.05). Serum ß2-microglobulin in chronic kidney disease group was significantly higher than those in PE and control groups [(4.0±2.6) vs (2.7±0.7) vs (2.0±0.5) mg/L; F=15.892, P<0.01]. Serum transferrin in chronic kidney disease group was significantly lower than those in PE and control groups [(3.0±0.8) vs (3.7±1.1) vs (3.6±0.6) g/L; F=6.284, P<0.01]. (3) The correlation between serum immunoglobulin, ß2-microglobulin, transferrin and blood pressure, proteinuria value and delivery weeks in PE group: the blood pressure level was not correlated with serum IgA, ß2-microglobulin and transferrin values in PE group (all P> 0.05). So, 24 hours proteinuria value was positively correlated with ß2-microglobulin (r=0.557, P<0.01), which was negatively correlated with transferrin (r=-0.442, P<0.01) and was not correlated with IgA(r=0.089, P=0.556). There was a negative correlation between delivery weeks and ß2-microglobulin (r=-0.328, P=0.026), and positive correlation with transferrin (r=0.315, P=0.035) and no correlation with IgA (r=-0.169, P=0.260). (4) The correlation between serum immunoglobulin, ß2-microglobulin, transferrin and blood pressure, proteinuria value and delivery weeks in chronic kidney disease group: the blood pressure level was positively correlated with ß2- microglobulin (systolic pressure: r=0.598, P<0.01; diastolic pressure:r=0.557, P<0.01), which was not correlated with IgA and transferrin in chronic kidney disease group (all P>0.05). So, 24 hours proteinuria value was positively correlated with ß2-microglobulin and IgA (r=0.568, r=0.330, both P<0.05), and not correlated with transferrin (r=0.255, P=0.133). Delivery weeks had a negative correlation with ß2-microglobulin (r=-0.574, P<0.01), while it had a positive correlation with transferrin (r=0.369, P=0.027). No correlation was found between delivery weeks and IgA values (r=-0.257, P=0.131). Conclusion: The serum levels of IgA, ß2-microglobulin and transferrin in PE and pregnancies with chronic kidney disease are significantly different, which may provide clinical value for the diagnosis of PE and pregnancies with chronic kidney disease in future.


Assuntos
Pressão Sanguínea/fisiologia , Imunoglobulinas/sangue , Pré-Eclâmpsia/sangue , Complicações Cardiovasculares na Gravidez/sangue , Insuficiência Renal Crônica/metabolismo , Transferrina/análise , Microglobulina beta-2/análise , Estudos de Casos e Controles , Feminino , Humanos , Hipertensão/sangue , Parto , Gravidez , Transferrina/metabolismo
12.
J Periodontal Res ; 53(1): 22-28, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28795395

RESUMO

BACKGROUND AND OBJECTIVE: This study aimed to develop pH-responsive polylactide-glycolic acid co-polymer and chitosan (PLGA/chitosan) nanosphere as an inflammation-responsive vehicle and evaluate the potential of the nanosphere encapsulating metronidazole, an antibiotic, and N-phenacylthiazolium bromide (PTB), a host modulator, for treating periodontitis. MATERIAL AND METHODS: PLGA/chitosan nanospheres were fabricated using oil-in-water emulsion method. Experimental periodontitis was induced on the rat maxillae, and the sites were randomly allocated to four treatment categories, including periodontitis alone (PR), periodontitis with nanospheres alone, nanospheres encapsulating metronidazole (MT) and nanospheres encapsulating PTB (PB). The ligature was retained until the animals were killed, and the treatment outcome was evaluated by the progression of periodontal bone loss (PPBL), inflammatory cell infiltration and collagen deposition. RESULTS: The encapsulated drug was released rapidly from the nanospheres without significant initial burst release at pH 5.5. Compared with group PR, PPBL was significantly reduced in groups MT and PB on day 4 (P<.05). On day 21, PPBL was significantly lower in group PB (P<.05). In groups MT and PB, inflammation was significantly reduced in groups MT and PB relative to groups PR and periodontitis with nanospheres alone (P<.05), and collagen deposition was significantly greater relative to group PR (P<.05). CONCLUSION: PLGA/chitosan nanospheres encapsulating metronidazole or PTB showed potential for modulating periodontitis progression.


Assuntos
Anti-Infecciosos/farmacologia , Metronidazol/farmacologia , Nanosferas , Periodontite/prevenção & controle , Tiazóis/farmacologia , Animais , Quitosana , Modelos Animais de Doenças , Progressão da Doença , Ácido Láctico , Ácido Poliglicólico , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Ratos Sprague-Dawley
13.
Braz J Med Biol Res ; 50(12): e6145, 2017 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-29069222

RESUMO

Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 µg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF). Cytotoxicity was assessed qualitatively by morphologic assessment and quantitatively by MTT assay. Expressions of proinflammatory mediators were assessed using quantitative real-time PCR and production of NO was quantified. Phosphorylation of AKT and nuclear localization of nuclear factor kappa B (NF-κB) were examined using western blotting. Exposure of HUVECs to RA-supplemented HDF had no influence on morphology and viability. Inhibition of proinflammatory mediator production in HUVECs by RA supplementation to HDF was significant in a dose-dependent manner. Exposure to RA-supplemented HDF resulted in a decrease in nitric oxide synthase expression and reduction of NO production in LPS-stimulated HUVECs. RA supplementation of HDF suppressed Akt activation in LPS-stimulated HUVECs. In addition, the level of cellular IκB was increased in parallel to a reduced nuclear translocation of NF-κB in LPS-induced endothelial cells. Our results suggest that RA-supplemented HDF is biocompatible and significantly suppressed inflammation induced in endothelial cells. In this respect, the use of HDF supplemented with RA could alleviate inflammation and improve long-term treatment of patients with renal failure on dialysis. Further clinical studies are required to confirm the effects.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Materiais Biocompatíveis/farmacologia , Cinamatos/farmacologia , Depsídeos/farmacologia , Soluções para Hemodiálise/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Inflamação/tratamento farmacológico , Análise de Variância , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Citocinas/análise , Citocinas/efeitos dos fármacos , Formazans , Soluções para Hemodiálise/química , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Immunoblotting , Inflamação/metabolismo , Lipopolissacarídeos , NF-kappa B/análise , Óxido Nítrico/análise , Fosforilação , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Sais de Tetrazólio
14.
Zhonghua Shao Shang Za Zhi ; 33(7): 431-436, 2017 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-28763910

RESUMO

Objective: To explore the influence of different inner dressings in negative-pressure wound therapy (NPWT) on escharectomy wound of full-thickness burn rabbits. Methods: Eighteen Japanese white rabbits were inflicted with full-thickness burn on unilateral back. They were divided into polymer dressing group (PD), biological dressing group (BD), and silver biological dressing group (SBD), according to the random number table, with 6 rabbits in each group. On 3 days post burn, the wounds were performed with escharectomy, and then wounds of rabbits in group PD were covered with polyurethane foam. Wounds of rabbits in group BD were covered with porcine acellular dermal matrix (ADM) and wounds of rabbits in group SBD were covered with silver porcine ADM. Then continuous NPWT was performed on rabbits of the three groups for 7 days. Immediately after surgery and on post surgery day (PSD) 7, general observation of wound was conducted and tissue around the wound was harvested for determination of dry to wet weight ratio. The content of bacteria was counted and the content of tumor necrosis factor α (TNF-α), interleukin-1ß (IL-1ß), and IL-6 in wound was determined by enzyme-linked immunosorbent assay. Fibroblasts in wound were counted after Masson staining and number of microvessels was counted after CD31 antibody immunohistochemical staining. Data were processed with analysis of variance for repeated measurement, LSD-t test, paired samples t test, and Bonferroni correction. Results: (1) Immediately after surgery, there was no granulation tissue in basal wound of rabbits in the three groups, with rich blood supply and obvious edema. On PSD 7, much granulation tissue was found in basal wound of rabbits in the three groups, with no or mild edema and no obvious redness and swelling in wound edge. (2) There were no significant differences in dry to wet weight ratios of tissue around the wound among and within the three groups immediately after surgery and on PSD 7 (with F values respectively 0.70 and 0.09, t values from 0.17 to 0.52, P values above 0.05). (3) Immediately after surgery, the content of bacteria in wounds of rabbits in groups PD, BD, and SBD was respectively (603.0±146.0) ×10(4,) (573.0±63.0) ×10(4,) and (590.0±100.0)×10(4) colony-forming unit (CFU)/g, with no significant difference among them (F=0.13, P>0.05). On PSD 7, the content of bacteria in wounds of rabbits in groups PD, BD, and SBD were respectively (5.4±0.8) ×10(4,) (4.6±0.9) ×10(4,) and (3.5±0.9)×10(4) CFU/g. Among them, the content of bacteria in wounds of rabbits in group SBD was lower than that in groups PD and BD, respectively (with t values respectively 3.78 and 2.29, P<0.05 or P<0.01). The content of bacteria in wounds of rabbits in the three groups on PSD 7 was decreased compared with that immediately after surgery (with t values from 10.05 to 21.81, P values below 0.01). (4) There was no significant difference in content of TNF-α, IL-1ß, and IL-6 in wounds of rabbits in the three groups immediately after surgery and on PSD 7 (with F values from 0.10 to 1.89, P values above 0.05). The content of TNF-α in wounds of rabbits in the three groups on PSD 7 was significantly higher than that immediately after surgery (with t values from 2.93 to 5.01, P<0.05 or P<0.01). (5) There was no significant difference in amount of fibroblasts in wounds of rabbits in the three groups immediately after surgery and on PSD 7 (with F values respectively 0.01 and 0.81, P values above 0.05). The amount of fibroblasts in wounds of rabbits in the three groups on PSD 7 was larger than that immediately after surgery (with t values from 4.78 to 11.58, P values below 0.01). (6) There was no significant difference in number of microvessels in wounds of rabbits in the three groups immediately after surgery and on PSD 7 (with F values respectively 2.42 and 2.49, P values above 0.05). The number of microvessels in wounds of rabbits in the three groups on PSD 7 was larger than that immediately after surgery (with t values from 7.17 to 11.14, P values below 0.01). Conclusions: SBD is better at inhibiting the growth of bacteria. PD, BD, and SBD have almost the same effects on reducing tissue edema and inflammatory reaction, and on promoting the accumulation of collagen fibers and tissue vascularization.


Assuntos
Derme Acelular , Bandagens , Queimaduras/terapia , Tratamento de Ferimentos com Pressão Negativa , Cicatrização , Animais , Curativos Biológicos , Ensaio de Imunoadsorção Enzimática , Tecido de Granulação , Inflamação , Interleucina-1beta , Interleucina-6 , Coelhos , Lesões dos Tecidos Moles , Resultado do Tratamento , Fator de Necrose Tumoral alfa
15.
Zhonghua Er Ke Za Zhi ; 55(6): 462-467, 2017 Jun 02.
Artigo em Chinês | MEDLINE | ID: mdl-28592016

RESUMO

Objective: To evaluate the efficacy and safety of oseltamivir in the treatment of suspected influenza in children. Method: A multicenter, randomized and open-label trial was conducted among 229 individuals with suspected influenza which were collected from the clinic of 5 hospitals in Guangdong province (Guangzhou Women and Children's Medical Center, Shenzhen Baoan District Maternity and Child Care Service Center, the Second Affiliated Hospital of Shantou University Medical College, Dongguan Maternity and Child Care Service Centre, Yuexiu District Children's Hospital of Guangzhou) from April to July 2015. They were randomized either to oseltamivir group (oseltamivir 30-75 mg, twice daily for 5 days) or control group who were given symptom relief medicines for 5 days. Result: No significant difference was found between two groups in influenza symptoms of the patients before the treatment(P>0.05). Altogether 229 individuals (114 in oseltamivir group, 115 in control group) were analyzed for efficacy, in which 73 individuals (42 oseltamivir, 31 control), 31.9%, were identified as influenza-infected through laboratory test. No significant difference was found between the two groups in the duration of fever although shortened. In the 229 individuals , the cumulative alleviation proportion between oseltamivir and control group was not significantly different (P>0.05): the median duration of illness was 69.9 hours (95% CI 65.3-91.5) in oseltamivir group and 75.4 hours (95%CI 63.9-91. 7) in control group; the median duration of fever was 40.4 hours (95%CI 31.5-53.4) in oseltamivir group and 44.0 hours (95%CI 33.2-50.0) in control group. In the 73 individuals, the cumulative alleviation proportion between oseltamivir and control group was significantly different (P<0.05). The median duration of illness was 61.2 hours (95%CI 48.0-121. 0) in oseltamivir group, being significantly shorter than that of 116.0 hours (95%CI 91.5-175.0) in control group. But it was not significantly different that the median duration of fever was 32.8 hours (95%CI 24.0-47.0 ) in oseltamivir group and 55.8 hours (95%CI 43.6-78.3 ) in control group (P>0.05). And the median duration of fever in 60 individuals (38 oseltamivir, 22 control) was significantly different between two groups(P<0.05), who had finished a course of taking oseltamivir in the 73 individuals, 34.8 hours (95%CI 24.0-48.5 ) in oseltamivir group being significantly shorter than that of 53.3 hours (95%CI 43.6-104.0 ) in control group. There was certain difference in side effects rate between the two groups (oseltamivir 10%, control 2%, P<0.05). The main side-effects were gastrointestinal symptoms (stomachache, diarrhea, poor appetite, vomiting). Conclusion: The duration of illness and fever in suspected influenza patients treated with oseltamivir was shorter than those in the patients treated with no oseltamivir, the difference was not statistically significant, when 31.9% was confirmed with positive result of virus test in suspected influenza in children. But in these patients with positive result of virus test, the duration of illness was significantly shortened with treatment with oseltamivir as compared with no treatment with oseltamivir, and it would be better if full oseltamivir course was completed for reducing the duration of fever. Oseltamivir treatment was safe with mild side effects.


Assuntos
Antivirais/uso terapêutico , Influenza Humana/tratamento farmacológico , Oseltamivir/uso terapêutico , Dor Abdominal , Antivirais/efeitos adversos , Criança , Diarreia , Esquema de Medicação , Feminino , Febre , Hospitais Pediátricos , Humanos , Masculino , Oseltamivir/efeitos adversos , Resultado do Tratamento , Vômito
16.
Zhonghua Wai Ke Za Zhi ; 55(6): 406-409, 2017 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-28592071

RESUMO

At present, several clinical practice guidelines for the treatment of osteoarthritis have been developed by institutes or societies. The ultimate purpose of developing clinical practice guidelines is to formulate the process in the treatment of osteoarthritis effectively. However, the methodologies used in developing clinical practice guidelines may place an influence on the transformation and application of that in treating osteoarthritis. The present study summarized the methodological features of individual clinical practice guideline and presented the tools for quality evaluation of clinical practice guideline. The limitations of current osteoarthritis guidelines of China are also indicated. The review article might help relevant institutions improve the quality in developing guide and clinical transformation.


Assuntos
Osteoartrite/terapia , Guias de Prática Clínica como Assunto , China , Humanos
17.
Insect Mol Biol ; 26(5): 564-573, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28621439

RESUMO

Antimicrobial peptides (AMPs) are small-molecule peptides that play crucial roles in insect innate immune responses. To better understand the function of AMPs in Plutella xylostella, one of the main pests of cruciferous vegetables, three full-length cDNAs encoding moricins were cloned from Pl. xylostella. Two variants of the moricin named PxMor2 and PxMor3 were heterologously expressed and purified. A secondary structure analysis using circular dichroism demonstrated that the two peptides adopted an α-helical structure in the membrane-like environment, but in aqueous solution, they were present in random coiled conformation. Antimicrobial activity assays demonstrated that PxMor2 exhibited high activity against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli; however, PxMor3 only demonstrated high activity against E. coli. Scanning electron microscopy and confocal laser-scanning microscopy analyses suggest that PxMors can lead to the disruption of bacterial membrane, which might be the mechanism by which PxMors inhibit bacterial growth. This study contributes to the understanding of Pl. xylostella AMPs and immune responses, and also enriches the knowledge of insect moricin.


Assuntos
Peptídeos Catiônicos Antimicrobianos/farmacologia , Membrana Celular/efeitos dos fármacos , Proteínas de Insetos/genética , Mariposas/genética , Animais , Peptídeos Catiônicos Antimicrobianos/genética , Peptídeos Catiônicos Antimicrobianos/isolamento & purificação , Dicroísmo Circular , Escherichia coli/efeitos dos fármacos , Proteínas de Insetos/isolamento & purificação , Proteínas de Insetos/metabolismo , Testes de Sensibilidade Microbiana , Mariposas/imunologia , Mariposas/metabolismo , Estrutura Secundária de Proteína , Análise de Sequência de DNA , Staphylococcus aureus/efeitos dos fármacos
18.
Hong Kong Med J ; 23(4): 333-9, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28278491

RESUMO

INTRODUCTION: The need for better durability and longevity in total hip arthroplasty for patients with various hip joint diseases remains a challenge. This study aimed to obtain medium-term results at a follow-up of >10 years for Zweymüller-Plus total hip arthroplasty with ceramic-on-polyethylene bearing. METHODS: A retrospective study was conducted to review the results after a minimum of 12.4 years of 207 consecutive total hip arthroplasties in 185 patients in Peking University People's Hospital in China using the Zweymüller SL-Plus stem in combination with the Bicon-Plus threaded cup and ceramic-on-polyethylene bearing between October 1994 and April 2000. RESULTS: During the study period, two patients (2 hips) died and 25 patients (28 hips) were lost to follow-up. Two hips were revised for aseptic loosening of the Bicon-Plus cup. The mean clinical and radiological follow-up was 14.1 years (range, 12.4-16.5 years) for the remaining 156 patients (175 hips). The mean (standard deviation) Harris Hip score for the 175 hips increased significantly from 39.3 (3.8) preoperatively to 94.1 (2.5) postoperatively at a mean follow-up of 14.1 years (P<0.05). Focal osteolysis was observed in seven (4.0%) of 175 stems and three (1.7%) of 175 cups. The Kaplan-Meier survival with revision for any reason as the end-point was 99.03% (95% confidence interval, 95%-100%). CONCLUSIONS: The high survival rate of the cementless Zweymüller-Plus system with ceramic-on-polyethylene bearing at mid-term follow-up makes this total hip arthroplasty system reliable for patients with various hip joint diseases.


Assuntos
Artroplastia de Quadril/instrumentação , Cerâmica , Prótese de Quadril/estatística & dados numéricos , Artropatias/cirurgia , Polietileno , Adulto , Idoso , Artroplastia de Quadril/métodos , China , Feminino , Seguimentos , Articulação do Quadril/fisiopatologia , Humanos , Artropatias/fisiopatologia , Perda de Seguimento , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Desenho de Prótese , Falha de Prótese , Amplitude de Movimento Articular , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
19.
J Anim Physiol Anim Nutr (Berl) ; 101(5): e342-e351, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28063238

RESUMO

The potential benefits of Aspergillus-fermented mung bean seed coats (FMSC) for weaned pigs remain unexplored. Both in vitro and in vivo experiments were employed to evaluate the potential of FMSC supplement on the growth, antioxidant and immune responses of weaned pigs. The total polyphenols and DPPH scavenging capability of ethanol extract of FMSC exhibited a greater (p < 0.01) increase than those of pre-fermentation. With the addition of the polyphenol of FMSC extract, an increase in phagocytosis by neutrophils and proliferation of peripheral blood mononuclear cells (PBMC) were found. However, these observations were significantly inhibited (p < 0.05) in those activated cells. Next, 96 weaned pigs were allotted with a randomized complete block design into four dietary treatments, including 0 (control), 600, 1200 or 1800 mg/kg FMSC in a corn-soya bean meal basal diet for a 35-day trial. The pigs were injected with swine enzootic pneumonia (SEP) vaccines at day 3 and day 21 respectively. The results showed that dietary treatment failed to affect growth performance or serum SEP titre. The diet supplemented with 600-1800 mg/kg FMSC decreased faecal lactoferrin on day 21 and increased plasma trolox equivalent antioxidant capacity (TEAC) and erythrocytes catalase activity, as well as decreased (p < 0.01) plasma malondialdehyde (MDA) concentration on day 35. Diet supplementation of 1800 mg/kg FMSC increased phagocytosis by neutrophils and PBMC proliferation induced by pokeweed mitogen (PWM). However, the polymorphonuclear leucocytes (PMN)-positive respiratory burst cells were decreased in the supplementation of 1200 or 1800 mg/kg FMSC respectively. In addition, the serum haptoglobin concentration was decreased in the supplementation with 1200 mg/kg FMSC. Taken together, FMSC enriches polyphenols with antioxidative and immune modulated properties. After feeding FMSC, an improvement in antioxidative capability and immunocompetence was found, implying that FMSC could provide as a feed additive at optimal level 1200 mg/kg for weaned pigs.


Assuntos
Antioxidantes/metabolismo , Aspergillus/metabolismo , Sementes/química , Suínos/metabolismo , Vigna/química , Animais , Anticorpos Antivirais/sangue , Vacinas Bacterianas/imunologia , Eritrócitos/enzimologia , Fezes/química , Fermentação , Manipulação de Alimentos , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Lactoferrina/química , Lactoferrina/metabolismo , Fagocitose , Pneumonia Suína Micoplasmática/prevenção & controle , Superóxido Dismutase-1/metabolismo , Suínos/imunologia
20.
Zhonghua Yi Xue Za Zhi ; 97(1): 29-32, 2017 Jan 03.
Artigo em Chinês | MEDLINE | ID: mdl-28056287

RESUMO

Objective: To assess the cost of illness, the current medication treatment status and health service utilization of patients with knee osteoarthritis (OA). Methods: From January 2014 to May 2015, 250 patients in Arthritis Clinic and Research Center, Peking University People's Hospital were recruited and followed up for 12 months. There were four visits during the follow-up, i. e. baseline, 1, 6 and 12 month. Data on sociodemographic variables, direct cost of illness, medication utilization and health service utilization were obtained by the questionnaire for the analyses. Results: Two hundred and twelve participants had accomplished the 12-month follow-up. The direct cost of knee OA was (8 858±5 120) yuan per year , median number was 7 020 yuan. Medical-related cost was (7 184±4 890) yuan per year , median number was 5 420 yuan; comprising 81.1% of the direct cost, whereas the non-medical related cost was( 1 674±924) yuan(RMB) per year , median number (1 239) yuan, comprising 18.9% of the direct cost. The medication cost was (5 484±3 510) yuan , median number was 4 046 yuan, accounting for the largest proportion (76.3%) of the medical-related cost. As for the medication utilization, glucosamine was most commonly prescribed (64.8%), followed by the Chinese herbals (61.2%) and NSAIDs (48.8%). The average amount of the hospital-visit per year was (11.8±4.1). Most knee OA patients (68.8%) took the tertiary care hospital as their first choice when they consulted orthopedists or physicians for OA. Conclusion: The direct cost of OA patients is considerable. OA patients may take medicine blindly and not follow the current guidelines. The prevention and treatment of OA needs to be further standardized in the future.


Assuntos
Osteoartrite do Joelho , Custos de Medicamentos , Serviços de Saúde , Humanos , Osteoartrite , Osteoartrite do Joelho/economia , Inquéritos e Questionários
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