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1.
Dalton Trans ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32129387

RESUMO

A series of host-guest materials containing polyoxometalate anions and lanthanide-organic layers have been synthesized and structurally characterized. By anion-π interactions between the anions and the π-acidic naphthalenediimide moieties, the materials emit strong red room-temperature phosphorescence and exhibit reversible photochromism.

2.
Chem Commun (Camb) ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32003370

RESUMO

Anionic POMs prefer to bond with positive metal cations instead of neutral or negative organic ligands. Therefore, it is challenging to synthesize POM-based MOFs, let alone bi-POM-based host-guest MOFs. In this work, an unprecedented bi-POM-based host-guest MOF, Na[Ni(enMe)2]4[Ni(enMe)2(H2O)2]2{[Ni6(µ3-OH)3(enMe)3 (SIP)1.5(B-α-PW9O34)]2[H3PNiW11O40]}·5enMe·33H2O (1), with Ni6-capped [PW9O34] as the node of the host framework and Keggin-type [PNiW11O40] units as the guest was synthesized. 1 showed excellent chemical stability towards aqueous solutions of pH 2-12 at both ambient and boiling temperature, providing opportunities for its application in fresh water harvesting from air.

3.
Front Med ; 14(1): 43-50, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31907860

RESUMO

An effective eradication therapy of Helicobacter pylori (H. pylori) should be used for the first time. In this study, we assessed whether tailored therapy based on antibiotic susceptibility testing is more effective than traditional therapy. We also evaluated the factors that cause treatment failure in high-resistance areas. For this multicenter trial, we recruited 467 H. pylori-positive patients. The patients were randomly assigned to receive tailored triple therapy (TATT), tailored bismuth-containing quadruple therapy (TABQT), or traditional bismuth-containing quadruple therapy (TRBQT). For the TATT and TABQT groups, antibiotic selection proceeded via susceptibility testing using an agar-dilution test. The patients in the TRBQT group were given amoxicillin, clarithromycin, esomeprazole, and bismuth. Successful eradication was defined as a negative 13C-urea breath test at least eight weeks after the treatment ended. Susceptibility testing was conducted using an agar-dilution test. The eradication rate was examined via intention-to-treat (ITT) and per-protocol (PP) analyses. The clarithromycin, levofloxacin, and metronidazole resistance rates were 26.12%, 28.69%, and 96.79%, respectively. Resistance against amoxicillin and furazolidone was rare. The eradication rates for TATT, TRBQT, and TABQT were 67.32%, 63.69%, and 85.99% in the ITT analysis (P 0.001) and 74.64%, 68.49%, and 91.22% in the PP analysis (P 0.001), respectively. The efficacy of TABQT was affected by clarithromycin resistance, and bismuth exerted a direct influence on TATT failure. TABQT was the most efficacious regimen for use in high-resistance regions, especially among clarithromycin-susceptible patients.

4.
Sci Total Environ ; 708: 134839, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31785901

RESUMO

Microplastics (MPs) are widespread in marine and estuarine environments, but the contamination of MPs in mangrove wetlands is relatively unknown. Here, we quantify the presence of MPs in fish collected from Zhanjiang mangrove wetland, the largest mangrove in South China, which provide baseline data on MPs accumulation in fish in mangrove environment as the first evidence in China. MPs were found in 30 out of 32 fish species at an average abundance of 2.83 ±â€¯1.84 items individual-1, ranged from 0.6 to 8.0 items individual-1 in each species. MPs were detected in gills, stomach and intestine, and not found in muscles and livers. Positive relationship was found between MPs abundance and body length or weight of mangrove fish. The dominant polymers identified by micro-FTIR were polyethylene, polyethylene terephthalate, polypropylene and cellophane. MPs consisted primarily of fibers and with the prominent size range of 0.02-1 mm. The body sizes, living habitats and feeding habits of fish are important factors affecting MPs accumulation in different fish species. This study revealed the wide presences of MPs in fish species within a mangrove wetland.

5.
Environ Pollut ; 258: 113734, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31884260

RESUMO

Microplastics are widespread across the global oceans, yet the potential risks of the ubiquitous environmental contaminant to marine organisms has been less known. Accumulation of microplastics and associated contaminants in marine fish, may pose adverse impacts to human health via seafood consumption. This study evaluated microplastic contamination in 24 fish species collected from Beibu Gulf, one of the world's largest fishing grounds in South China Sea. Microplastics were detected in 12 fish species at an abundance of 0.027-1.000 items individual-1 and found in fish stomach, intestines and gills with the count percentage of 57.7%, 34.6% and 7.7%, respectively. Transparent fibers were observed as the predominant microplastics, which might be ingested accidently by fish or transferred through other animals at lower trophic levels. Majority of microplastics were identified as polyester (44%) and nylon (38%), whereas polypropylene (6%), polyethylene (6%), and acrylics (6%) were also found. Relatively, higher microplastic abundances were found in demersal fish compared to the pelagic species. Overall, the abundance of microplastics was documented as relatively low in the commercial fish collected from the open water of Beibu Gulf, South China Sea.

6.
Molecules ; 24(22)2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31731832

RESUMO

This research aimed to investigate the immunomodulatory effects of phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) in immunosuppressed mice, improving their cellular and humoral immune function. Our results showed that pRCPS increased serum immunoglobulin (IgG, IgA, IgM) concentrations significantly, enhanced splenocyte proliferation, and the thymus and spleen indices. pRCPS also promoted phagocytosis in peritoneal macrophages and enhanced cytokine (IFN-γ, IL-2, -4, -5, -6, and -10) serum levels. Importantly, pRCPS increased the proportions of selected T cell subpopulations (CD3+, CD4+, and the CD4+ to CD8+ ratio). Our results revealed that phosphorylation of the polysaccharides promoted their immune-enhancing effects. Thus, pRCPS can enhance cellular and humoral immunity and could be used as an immune-enhancing agent to overcome cyclophosphamide (CY)-induced immunosuppression.

7.
Environ Sci Technol ; 53(12): 6804-6813, 2019 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-31117546

RESUMO

Although the distribution of progestagens in aquatic environments has been widely reported, details on their uptake, elimination, and biotransformation in fish have received little attention. This study investigated the uptake, elimination, and biotransformation potential of a progestagen, cyproterone acetate (CPTA), in Nile tilapia ( Oreochromis niloticus) exposed to an environmentally relevant concentration under semistatic regimes. CPTA in tilapia tissues followed a similar pattern, reaching a concentration plateau within 4 days of exposure, and dropping to below limits of quantitation within 4 days of elimination. The calculated steady-state bioconcentration factors suggest a low bioconcentration potential of CPTA in juvenile tilapia. Results of enzymatic hydrolysis treatments revealed that no conjugates of CPTA were present in tissues, but conjugated biotransformation products of CPTA were found in bile, liver, and muscle. Most CPTA entered tissues and then was biotransformed into seven different products by phase I and phase II metabolism. The concentrations of endogenous cortisol were significantly influenced by CPTA in plasma and liver during the uptake period. These findings suggest that biotransformation products of CPTA should be considered for the assessment of the bioconcentration potential and ecological effects of progestagens.


Assuntos
Tilápia , Poluentes Químicos da Água , Animais , Biotransformação , Acetato de Ciproterona , Progestinas
8.
J Environ Manage ; 241: 1-11, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30978564

RESUMO

Refining is the major process of paper formation. This study focuses on the impact of a specific enzyme (Paenibacillus cellulase) for fibers on the surfaces, the roughness and height of the fibers are also investigated. Effects of enzyme dosages and the mechanical refining action (PFI revolution) on fiber physical properties were also analyzed. The fibers were observed by scanning electron microscopy (SEM), their roughness and height were analyzed by Atomic force microscopy (AFM). Results show that the Paenibacillus cellulase pretreatment increased the drainability of both kinds of pulp at the same level of refining energy. In other words, enzymatic treatment on pulp refining consumed less refining revolutions to reach the same drainability compared to the untreated pulp. Although the viscosity of both kinds of pulp was degraded with the enzymatic treatment, the physical properties of paper had no significantly negative influence on them. The results indicated the treatment with cellulase swelled the fibers in the absence of refining, and there were better fibrillation on the fibers treated with cellulase after refining. Furthermore, the statistical analysis of AFM suggested that both kinds of pulp treated with low cellulase dosage with PFI refining had higher roughness.


Assuntos
Celulase , Paenibacillus , Carboidratos , Fibras na Dieta , Papel
9.
Anim Biotechnol ; 30(4): 358-365, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30179066

RESUMO

The low expression of exogenous transferred gene limited the application of transgenic animal technology. Suppressor of variegation 3 ∼ 9 homolog 1(SUV39H1) gene plays a prominent role on repressive heterochromatin and transcription. To understand if exogenous transgenic gene expression was affected by SUV39H1 epigenetic modification, in this paper, the effective shRNA fragments targeting SUV39H1 gene were first screened, their roles on expression of exogenous transgenic genes were determined by using Bcap-37 cell line with stable expressing IFNα-2b gene as a model, the preliminary regulation mechanism of SUV39H1 gene was investigated. The results showed that the designed shRNA1/2 fragments of SUV39H1 gene had an obvious inhibition effect on the expression of SUV39H1 gene, reached 53.07 and 31.28%, respectively by qRT-PCR analysis. Compared with the control group, the expression of IFNα-2b gene in transgenic Bcap-37 cells infected with shRNA1 and 2 viruses significantly increased by 96.25 and 121.08%, respectively (p < 0.05). In addition, the expression of DNMT1, HDAC1 and G9a gene in the shRNA infected cells reduced significantly, and the expression of the HAT1 gene increased significantly (p < 0.05). The above results indicated that the expression of exogenous transgenic gene could be promoted by suppressing SUV39H1 gene at the cell level.


Assuntos
Histona-Lisina N-Metiltransferase/antagonistas & inibidores , Interferon alfa-2/metabolismo , RNA Interferente Pequeno , Transgenes , Animais , Búfalos , Bovinos , Linhagem Celular Tumoral , DNA (Citosina-5-)-Metiltransferase 1/metabolismo , Expressão Gênica , Células HEK293 , Histona Acetiltransferases/metabolismo , Histona Desacetilase 1/metabolismo , Histona-Lisina N-Metiltransferase/genética , Histona-Lisina N-Metiltransferase/metabolismo , Humanos
10.
Chemosphere ; 215: 25-32, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30300808

RESUMO

Biodegradable plastics, as alternatives to conventional plastics, are increasingly used, but their interactions with organic pollutants are still unknown. In this study, the sorption and desorption behaviors on a type of biodegradable plastic-poly(butylene adipate co-terephtalate) (PBAT) were investigated, and at the same time two types of conventional plastics-polyethylene (PEc and PEv) and polystyrene (PS) were used for comparison. Phenanthrene (PHEN) was chosen as one of representative organic pollutants. Results indicated that the sorption and desorption capacities of PBAT were not only higher than those of the other types of microplastics, but also higher than those of carbonaceous geosorbents. The surface area normalized results illustrated that sorption and desorption of the microplastics were positively correlated with their abundance of rubbery subfraction. The sorption kinetic results showed that the sorption rates of PBAT and PEc were higher than PEv and PS. The effects of water chemistry factors including salinity, dissolved organic matter and Cu2+ ion on the sorption process displayed the same trend, but the degrees of influence on the four microplastics differed. The degrees of influence were mainly dependent on the abundance of rubbery subfraction for microplastics. These findings indicate that the biodegradable poly(butylene adipate co-terephtalate) microplastics are actually stronger vectors than the conventional microplastics, and crystallization characteristics of the microplastics have great influences on the vector effect.


Assuntos
Fenantrenos/química , Fenantrenos/isolamento & purificação , Plásticos/química , Poliésteres/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação , Adsorção
11.
Sci Total Environ ; 644: 375-381, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-29981986

RESUMO

Microplastics, as emerging contaminants in the global environment, have become a cause for concern for both academics and the public. The present understanding of microplastic pollution is primarily focused on marine environments, and less attention has been given to freshwater environments, in particular, to urban rivers. In this study, microplastics were sampled from surface water and sediments in 14 sites located in the lower course of the Pearl River. These sampling sites are located along Guangzhou of South China, with built-up areas being the dominant land use. The abundances of microplastics in surface water and sediments ranged from 379 to 7924 items·m-3 and 80 to 9597 items·kg-1, respectively. Polyethylene and polypropylene were the common types of microplastics, together accounting for 64.3% and 73.8% of surface water and sediment samples, respectively. Fibers were the dominant microplastic shapes in both water and sediment samples. The abundances of microplastics varied in surface water and sediments with each site, which might be affected by multiple factors. Our results indicated that wastewater treatment plants (WWTP) could reduce microplastics from municipal sewage which was finally discharged into the Pearl River along Guangzhou.

12.
World J Clin Cases ; 6(4): 54-63, 2018 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-29670890

RESUMO

AIM: To analyze the bacterial community structure and distribution of intestinal microflora in people with and without metabolic syndrome and combined these data with clinical indicators to determine relationships between selected bacteria and metabolic diseases. METHODS: Faecal samples were collected from 20 patients with metabolic syndrome and 16 controls at Cangnan People's Hospital, Zhejiang Province, China. DNA was extracted and the V3-V4 regions of the 16S rRNA genes were amplified for high throughput sequencing. Clear reads were clustered at the 97% sequence similarity level. α and ß diversity were used to describe the bacterial community structure and distribution in patients. Combined with the clinical indicators, further analysis was performed. RESULTS: Bacteroidetes, Firmicutes, Actinobacteria, Proteobacteria, Fusobacteria were the dominant phyla, and Prevotella, Bacteroides and Faecalibacterium was the top three genera in faecal samples. α diversity analysis showed that the species richness of metabolic syndrome samples (group D) was significantly higher than the control (group C) (P < 0.05), and the microbial diversity of group C was greater than that of group D. According to the principal co-ordinates analysis, the samples of group C clustered more tightly, indicating that the distribution of bacteria in healthy patients was similar. The correlation analysis showed that alkaline phosphatase was negatively correlated with the abundance of Prevotella (P < 0.05). There was a negative correlation between low-density lipoprotein and the abundance of Ruminococcus (P < 0.05) and a positive correlation between the high-density lipoprotein and the abundance of Ruminococcus (P < 0.05). The total protein and the alanine aminotransferase was positively correlated with the abundance of Peptostreptococcus (P < 0.05). CONCLUSION: The changes microbial communities can be used as an indicator of metabolic syndrome, and Prevotella may be a target microorganism in patients with metabolic syndrome.

13.
Environ Pollut ; 236: 619-625, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29433102

RESUMO

As a transitional zone between riverine and marine environments, an estuary plays an important role for the sources, accumulation and transport of microplastics. Although estuarine environments are hotspots of microplastic pollution, the correlation between microplastic pollution and aquatic organisms is less known. Here we investigated microplastic pollution in wild oysters Saccostrea cucullata from 11 sampling sites along the Pearl River Estuary in South China. The microplastic abundances in oysters ranged from 1.4 to 7.0 items per individual or from 1.5 to 7.2 items per gram tissue wet weight, which were positively related to those in surrounding waters. The oysters near urban areas contained significantly more microplastics than those near rural areas. Fibers accounted for 69.4% of the total microplastics in oysters. Microplastic sizes varied from 20 to 5000 µm and 83.9% of which were less than 100 µm. Light color microplastics were significantly more common than dark color ones. Based on the results, oysters are recommended as a biomonitor for the microplastic pollution in estuaries.


Assuntos
Monitoramento Ambiental , Ostreidae/química , Plásticos/análise , Poluentes Químicos da Água/análise , Animais , Organismos Aquáticos , China , Estuários , Rios
14.
Inflamm Bowel Dis ; 23(9): 1592-1599, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28570428

RESUMO

BACKGROUND: NUDT15 c.415C>T was a novel genetic marker confirmed in our center for thiopurine-induced leukopenia in Chinese inflammatory bowel disease (IBD). For validation, a large cohort study is needed. Meanwhile, the newly discovered NUDT15 coding variants (c.36_37insGGAGTC and c.52 G>A) have not been studied in patients with IBD. We aimed to further confirm the influence of 3 NUDT15 variants (c.415C>T, c.36_37insGGAGTC, and c.52G>A) on thiopurine-induced leukopenia in Chinese patients with IBD. METHODS: Patients prescribed on thiopurines for at least 2 weeks were recruited from 4 tertiary hospitals. Clinical data were collected. NUDT15 genotypes were determined with polymerase chain reaction-RFLP and sequencing. The interactions between variants and leukopenia were analyzed. RESULTS: A total of 732 patients were included, 177 (24.3%) of whom developed leukopenia. There were strong associations of NUDT15 c.415C>T, c.36_37insGGAGTC, and c.52G>A with thiopurine-induced leukopenia (P = 1.81 × 10, P = 4.74 × 10 and P = 0.04, respectively), whereas there was no relevance for thiopurine S-methyltransferase genotypes (P = 0.25). The predictive sensitivity of NUDT15 c.415C>T was 49.2%, whereas it increased to 55.4% when combined analysis with c.36_37insGGAGTC and c.52G>A. Notably, not only the homozygotes with NUDT15 c.415C>T but also the heterozygotes both carrying c.415C>T and c.52G>A developed early leukopenia. The median dosage for NUDT15 c.415C>T carriers was significantly lower than that for wild-type (P < 0.001). CONCLUSIONS: We confirmed that NUDT15 c.415C>T, c.36_37insGGAGTC, and c.52G>A variants were risk factors for thiopurine-induced leukopenia. Combined detection of the 3 variants could increase the predictive sensitivity of thiopurine-induced leukopenia and help to distinguish early leukopenia in heterozygote of c.415C>T in Chinese patients with IBD. Treatment monitoring by NUDT15 variants may be promising in individualized therapy.


Assuntos
Imunossupressores/efeitos adversos , Doenças Inflamatórias Intestinais/genética , Leucopenia/genética , Metiltransferases/efeitos adversos , Pirofosfatases/genética , Adolescente , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático , Azatioprina/efeitos adversos , Criança , Pré-Escolar , China , Estudos de Coortes , Feminino , Frequência do Gene , Predisposição Genética para Doença , Variação Genética , Heterozigoto , Homozigoto , Humanos , Doenças Inflamatórias Intestinais/tratamento farmacológico , Leucopenia/induzido quimicamente , Masculino , Mercaptopurina/efeitos adversos , Pessoa de Meia-Idade , Fatores de Risco , Adulto Jovem
15.
Pediatr Dermatol ; 32(6): e277-82, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26459993

RESUMO

Congenital hemidysplasia with ichthyosiform nevus and limb defects (CHILD) syndrome is an X-linked autosomal dominant disorder characterized by unilateral congenital hemidysplasia with ichthyosiform erythroderma and ipsilateral limb defects caused by a mutation in the gene encoding NAD[P]H steroid dehydrogenase-like protein (NSDHL) at Xq28. The histopathologic hallmark of skin lesions in CHILD syndrome is psoriasiform epidermis with hyperkeratosis and parakeratosis, and its most striking feature affecting the upper dermis is filling of the papillary dermis with foam cells. Here we present the case of a 9-year-old Chinese girl born with the typical clinical features of CHILD syndrome. Histologic and immunohistochemical evaluation of the skin lesions confirmed the diagnosis and led to identification of a heterozygous point mutation in exon 8 of the NSDHL gene. In addition, we provide a literature review of 26 unrelated CHILD syndrome patients from different countries, caused by 20 unique gene mutations occurring throughout the entire NSDHL gene, to promote understanding and provide a more comprehensive description of this unusual disorder.


Assuntos
3-Hidroxiesteroide Desidrogenases/genética , Anormalidades Múltiplas/genética , Grupo com Ancestrais do Continente Asiático/genética , Doenças Genéticas Ligadas ao Cromossomo X/genética , Eritrodermia Ictiosiforme Congênita/genética , Deformidades Congênitas dos Membros/genética , Mutação Puntual , Anormalidades Múltiplas/diagnóstico , Criança , China/epidemiologia , Éxons/genética , Feminino , Doenças Genéticas Ligadas ao Cromossomo X/diagnóstico , Humanos , Eritrodermia Ictiosiforme Congênita/diagnóstico , Imuno-Histoquímica , Deformidades Congênitas dos Membros/diagnóstico , Reação em Cadeia da Polimerase , Pele/patologia
16.
J Chem Inf Model ; 55(8): 1616-27, 2015 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-26131601

RESUMO

Discovery of high-affinity and high-selectivity agonists of 5-HT1AR has become very attractive due to their potential therapeutic effects on multiple 5-HT1AR-related psychological and neurological problems. On the basis of our previously designed lead compound FW01 (Ki = 51.9 nM, denoted as 9a in the present study), we performed large-scale molecular dynamics simulations and molecular docking operations on 5-HT1AR-9a binding. We found the flip-packing events for the headgroup of 9a, and we also found that its tail group could bind flexibly at the agonist-binding site of 5-HT1AR. By finely tuning the flip-packing phenomenon of the 9a headgroup and tuning the binding flexibility of 9a tail group, we virtually designed a series of new 9a derivatives through molecular docking operations and first-principles calculations and predicted that these newly designed 9a derivatives should be higher-affinity agonists of 5-HT1AR. The computational predictions on the new 9a derivatives have been confirmed by our wet-experimental studies as chemical synthesis, binding affinity assays, and agonistic-function assays. The consistency between our computational design and wet-experimental measurements has led to our discovery of higher-affinity agonists of 5-HT1AR, with ∼50-fold increase in receptor-binding affinity and ∼25-fold improvements in agonistic function. In addition, our newly designed 5-HT1AR agonists showed very high selectivity of 5-HT1AR over subtype 5-HT2AR and also over three subtypes of dopamine receptors (D1, D2, and D3).


Assuntos
Desenho de Drogas , Receptor 5-HT1A de Serotonina/metabolismo , Agonistas do Receptor 5-HT1 de Serotonina/química , Agonistas do Receptor 5-HT1 de Serotonina/farmacologia , Sítios de Ligação , Humanos , Modelos Moleculares , Receptor 5-HT1A de Serotonina/química , Receptor 5-HT2A de Serotonina/química , Receptor 5-HT2A de Serotonina/metabolismo , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo
17.
World J Gastroenterol ; 21(3): 944-52, 2015 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-25624729

RESUMO

AIM: To evaluate the efficacy of centralized culture and possible influencing factors. METHODS: From January 2010 to July 2012, 66452 patients with suspected Helicobacter pylori (H. pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy. Gastric mucosal biopsies were taken from the antrum for culture. These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time: 5, 24 and 48 h. The culture results were reported after 72 h and the positive culture rates were analyzed by a χ (2) test. An additional 5736 biopsies from H. pylori-positive patients (5646 rapid urease test-positive and 90 (14)C-urease breath test-positive) were also cultured for quality control in the central laboratory setting. RESULTS: The positive culture rate was 31.66% (21036/66452) for the patient samples and 71.72% (4114/5736) for the H. pylori-positive quality control specimens. In the 5 h transport group, the positive culture rate was 30.99% (3865/12471), and 32.84% (14960/45553) in the 24 h transport group. In contrast, the positive culture rate declined significantly in the 48 h transport group (26.25%; P < 0.001). During transportation, the average natural temperature increased from 4.67 to 29.14 °C, while the positive culture rate declined from 36.67% (1462/3987) to 24.12% (1799/7459). When the temperature exceeded 24 °C, the positive culture rate decreased significantly, especially in the 48 h transport group (23.17%). CONCLUSION: Transportation of specimens within 24 h and below 24 °C is reasonable and acceptable for centralized culture of multicenter H. pylori samples.


Assuntos
Serviços Centralizados no Hospital , Mucosa Gástrica/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/isolamento & purificação , Testes de Sensibilidade Microbiana , Manejo de Espécimes/métodos , Transportes , Biópsia , Serviços Centralizados no Hospital/organização & administração , China , Endoscopia Gastrointestinal , Estudos de Viabilidade , Infecções por Helicobacter/diagnóstico , Humanos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Temperatura Ambiente , Fatores de Tempo
18.
Int J Clin Exp Med ; 8(10): 19304-10, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26770568

RESUMO

In this study, we determine the effects of leukoaraiosis and lacunes on the clinical outcome and relative complications of acute ischemic stroke patients. From January 2007 to June 2008, 323 consecutive patients with acute brain infarction were prospectively studied. Leukoaraiosis was defined as moderate or severe white matter hyperintensities, and lacunes were counted as follows: none (0 lacune), few (1-2 lacunes), and many (≥3 lacunes). During a three-month period after the onset of stroke, patient outcomes and the incidence of complications were assessed. Backward stepwise logistic regression was used to identify the independent predictors of adverse outcome and complications after stroke. During the follow-up, 128 patients (39.63%) experienced adverse outcomes (i.e., death or disability), and 69 patients (21.36%) developed chest infections. Leukoaraiosis was an independent predictor of adverse outcome and chest infection (adverse outcome: odds ratio, 3.971, 95% confidence interval, 1.954-8.073; chest infection: odds ratio, 1.809, 95% confidence interval, 1.220-2.681), whereas no associations were observed between lacunes and adverse outcomes or complications after stroke. In conclusion, Leukoaraiosis but not lacunes was an independent predictor of adverse outcomes and chest infection in acute ischemic stroke patients. The difference between the effects of lacunes and leukoaraiosis may reflect the heterogeneity of these two primary features of small vessel diseases.

19.
Inorg Chem ; 53(10): 4794-6, 2014 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-24779641

RESUMO

Through judicious choice of the ligands and patient regulation of the solvent conditions, three unique chiral coordination polymeric isomers have been synthesized. Their structures, gas adsorption, and structural interconversion have been studied. One of the isomers displays dynamic behavior, and its use in the enantioselective separation of chiral alcohols has also been reported.

20.
Opt Express ; 22(4): 4751-67, 2014 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-24663794

RESUMO

Designated eye position (DEP) and viewing zone (VZ) are important optical parameters for designing a two-view autostereoscopic display. Although much research has been done to date, little empirical evidence has been found to establish a direct relationship between design and measurement. More rigorous studies and verifications to investigate DEP and to ascertain the VZ criterion will be valuable. We propose evaluation metrics based on equivalent luminance (EL) and binocular luminance (BL) to figure out DEP and VZ for a two-view autostereoscopic display. Simulation and experimental results prove that our proposed evaluation metrics can be used to find the DEP and VZ accurately.

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